biochrom Novaspec III Plus User Manual

5061-068 Rev 1.0

Novaspec III

+

Novaspec III+80-2120-40

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Table of Contents

Essential Safety Notes .....................................................................................................................................................4

Unpacking, Positioning, Installation ..............................................................................................................................4

Warranty and Repair ........................................................................................................................................................5

Technical Specifications ……………………………………………………………………………………………………………….6
Operation..……………………………………………………………………………………………………………………………….7

Introduction ………………………………………………………………………………………………………………….………7
Sample Handling Tips ..…………………………………………………………………………….………………………………7
Keypad and Display ..………………………………………………………………………………………………………………8
Software Style …………..………………………………………………………..…………………………………………………9

Parameters …….. …………………………………………………………………………………………………………………….10

Absorbance ………………..….……………………………..…………………………………………………………………….10
Transmission ……………………..………………………………………………………………………………………………..13
Concentration with a Factor …….………………………………………………………………………………………………..16
Concentration with a Standard .………………..………………………………………………………………………………..19

Rate …………………………………. ...…………………………………………………………………………………………..22

Methods Folder ………………………………………………………………………………………………………………………..25

Saving a Method …………………………………………….…………………………………………………………………….25
Folder Names……………………………………………………………………….……………………………………………..26
Lock Folder……………………………………………………………………….………………………………………………..27
Unlock Folder……………………………………………………………………….……………………………………………..27
Delete a Method ……………………………………………………………………….………………………………………….28
Lock Method……………………………………………………………………….………………………………………………28
Unlock Method……………………………………………………………………….……………………………………………29
When saving to a USB Memory Stick …………………………..………………………...…………………………………...30

Utilities Folder ………………………………………………………………………….………………………………….………….32

Date and Time …………………………………………………………………………………………………………………….33
Regional ……………………………………………………………………………………………………………………………33
Export Data …………………………………………………………………………..……………………………………………34
Preference …………………………………………………………………………………………………………………………34
Contrast ……………………………………………………………………….……………………………………………………35
About ………………………………………………………………………………………….…………………………………….35

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Print Via Computer ………………………………………………………...………………………………………………………….36

Installation ………………………………………………………………………………………………………………………….36
Test Tube and Heated Cell Holder Installation……………………………………………………………………………………..37
Accessories ……………………...…………………………………………………………………………………………………….38
Cleaning and General Care of the Instrument ……….……………..…..…………………………………………………………38

External cleaning ……………………………….……………………………………………………………...………………….38

Changing cell holder or removal for cleaning ……………………………………….……………………..…………………..38
Icon Glossary ………………………………………………………………………………………………………………………….39

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Essential Safety Notes

There are a number of warning labels and symbols on your instrument. These are there to inform you where potential
danger exists or particular caution is required. Before commencing installation, please take time to familiarize yourself
with these symbols and their meaning.

Caution (refer to accompanying documents).

Unpacking, Positioning and Installation

 Check the contents of the package against the packing list. If any shortages are discovered, inform your supplier

immediately.

 Inspect the instrument for any signs of damage caused in transit. If any damage is discovered, inform your supplier

immediately.

 Ensure your proposed installation site conforms to the environmental conditions for safe operation:

 Indoor use only.
 Temperature range 5°C to 35°C. Note that if you use the instrument in a room subjected to extremes of

temperature change during the day, it may be necessary to recalibrate (by switching off and then on again).

 Maximum relative humidity of 80% up to 31°C decreasing linearly to 50% at 40°C.

 The instrument must be placed on a stable, level bench or table that can take its weight (1.55 kg) to allow air to

circulate freely around the instrument.

 This equipment must be connected to the power supply with the power cord supplied. It can be used on 90 – 240 V~,

50-60 Hz supplies.

 If the instrument has just been unpacked or has been stored in a cold environment, it should be allowed to come to

thermal equilibrium for 2-3 hours in the laboratory before turning the instrument on. This will prevent calibration failure
as a result of internal condensation.

 Switch on the instrument via the keypad ( ) after it has been plugged in. The instrument will perform a series of

self-diagnostic checks.

 Please read through this user manual prior to use.
 Please contact your original supplier in the first instance if you experience technical or sample handling difficulties.

If this equipment is used in a manner not specified or in environmental conditions not appropriate for safe operation, the
protection provided by the equipment may be impaired and instrument warranty withdrawn.

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Warranty and Repair

Biochrom warrants this instrument for a period of 12 months (1 year) from the date of purchase.Where appropriate,
Biochrom will repair or replace the unit for defects of workmanship or materials. This warranty does not extend to damage
resulting from misuse, neglect, or abuse, normal wear and tear, or accidental damage. This warranty extends only to the
original consumer purchaser.

Products failing within the first 30 days of end user operation are considered dead on arrival (DOA) and where appropriate
a replacement will be given if a repair is not possible. In the instance of a DOA; Biochrom will incur shipping charges of
returning the unit back to the facility.

IN NO EVENT SHALL BIOCHROM BE LIABLE FOR INCIDENTAL OR CONSEQUENTAL DAMAGES. Some states do
not allow the exclusion or limitation of incidental or consequential damages so the above limitation to exclusion may not
apply to you. THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR USE,
OR OF ANY OTHER NATURE. Some states do not allow this limitation on an implied warranty, so the above limitation
may not apply to you.

If any defect arises within or outside the warranty period, please contact:

US Office Technical Support

Email [e-mail]

Telephone (Toll Free) 1-800-272-2775

Telephone (Outside the US) 1-508-893-8999

Address 84 October Hill Road

Holliston MA 01746

USA

UK Office Technical Support

Email [e-mail]

Telephone [phone]

Address 1020 Cambourne Business

Park
Cambourne
Cambridge UK
CB23 6DW

Goods will not be accepted for return unless a RMA (Returns Materials Authorization) number has been issued by our
Returns-Repairs Department. The customer is responsible for shipping charges. Please allow a reasonable period of time
for completion of repairs or replacement.

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Technical Specifications

Wavelength range

325nm to 1100nm

Monochromator

Flat grating

Wavelength calibration

Automatic upon switch on

Spectral bandwidth

<7nm

Wavelength accuracy

± 2nm

Wavelength reproducibility

± 1nm

Light sources

Pulsed Tungsten halogen

Detector

CMOS array

Photometric range

- 0.300 to 2.500A, 0.3 to 199%T

Photometric linearity

± 2.0 % or ± 0.010A to 1.000A at 546nm,
whichever is the greater

Photometric reproducibility

< 0.002 A at 0A and 500nm

Stray Light

< 1%T 340nm

Stability

± 0.005A/h at 0A and 546nm after warm-up

Noise

± 0.002A near 0A and ± 0.020A near 2A at
600nm

Digital output

USB memory stick data storage
USB PC connection with PVC software

Data Export

PC via USB cable
USB memory stick

Method Storage

90 with PIN number protection

Graphical Display

Yes, zoom and track function

Sample ID

Yes

Languages

English, German, French, Spanish, Italian,
Japanese, Chinese

Dimensions

120 x 230 x 320 mm

Weight

1.55 kg

Power input

18 Vdc at max 18VA from a supplied 100 to
240V~, 50/60 Hz Mains Power Adapter

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Operation

Introduction

Your spectrophotometer is a simple-to-use visible instrument. It has no moving parts, which is the basis of the rapid
scanning operating system. It has been designed to meet the routine spectroscopy needs of customers requiring a
compact instrument that is easy to use. The product is reliable and requires low maintenance.

After it is turned on and calibrated the Novaspec III+will show an absorbance measurement display.

To select different measurement modes, change parameters or change other settings, click on the menu key and
select the required menu item by clicking on the associated menu number using the keypad.

Measurement Modes Absorbance, Transmission, Concentration with a Factor, Concentration with a Standard,

Rate (Kinetics)

Methods Contains eight folders that can store less frequently used configured methods (eight

methods per folder)

Utilities Instrument set up (date, time, language, data output, preferences and display settings)

The instrument is supplied with a program, PVC (Print via Computer) on the accompanying CD. When used with a USB
cable to connect to a PC onto which the software has been installed, it enables the user to “print through” the PC directly
to the printer that is connected to it. The data may also be stored as an Excel spreadsheet, as an EMF graphics file, a
comma delimited (csv) data file, a tab delimited (txt) data file or in native PVC format for later access.

The instrument has a USB connector located at the front of the unit which allows data to be stored on a USB memory
stick. This data can be stored in a PVC format which can be opened using PVC software, a comma separated variable
(CSV) data file or a tab separated variable format which can be imported into Excel.

Sample Handling Tips

 The light beam is directed from RIGHT to LEFT through the cell chamber; therefore please ensure the cell is inserted

with the correct alignment.

 The cell holder supplied with the instrument accepts standard 10 mm path length quartz, glass or plastic cells.
 The optical height is 15 mm

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Keypadand Display

The back-lit liquid crystal display is very easy to navigate around using the alphanumeric entry and navigation arrow keys
on the hard wearing, spill proof membrane keypad.

Key Action

On/Standby
Key

Turns the instrument on or back to standby.

Arrow Keys

Use the four arrow keys to navigate around the display and select the required
setting for the active (highlighted) option.

View Options

Used to view current options.

Alphanumeric
Keys

Used to select items in a menu or to enter parameters and text descriptions
where. Repeated key presses can be used to cycle through lower case, upper
case, and numbers. Wait 1 second before entering the next character. Use the
C key to backspace and the 1 key to enter a space.

Escape/
Cancel

Escape from a selection and return to the previous screen.

OK

Confirm parameters/selection.

Set
Reference

Set reference to 0.000A or 100%T on a reference solution at the current
wavelength in the measurement mode selected. When in scan mode, do a
reference scan.

Take a
Measurement

Take a measurement.

On/Standby

Key

Escape/Cancel

OK Key

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Software Style

The user interface is built around the options key . The instrument will open automatically to an
application and you will use the options key to select different parameters.

Keypad Number Description

1 Parameter

Allows you to run in different modes: Absorbance, Transmission,
Concentration with Factor, Concentration with Standard, Rate
(Kinetics)

2 Print Export results via the selected method.

3 Graph

Allows you to toggle back and forth between how the results are
given, digital versus a graph.

4 Run Standard

Allows you to measure your standard when in Concentration
Standard measurement mode.

5 Utilities

Instrument set up (date, time, language, data output,
preferences and display settings).

6 Methods

Sub folder selection or user selected and configured method (8
sub folders each containing a maximum of 9 methods). To
return to the previous measurement mode, select the methods
followed by “Esc” button.

7 Sample Number Allows you to name your sample manually or can be prompted.

8 Save Method

Use the left and right arrow key to select a folder to store in and
using the alphanumeric keypad to name your method.

9 Auto-Print Toggles auto-print on/off.

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Step 1

Press the Options Key .

Step 2

Press 1 to set parameters.

Step 3

If not already in Absorbance mode, use left or right
arrow keys to select Absorbance mode.
Press the down arrow key.

Step 4

Use the left and right arrow keys to change the
wavelength, or use the number keypad.
Press the down arrow key.

Step 5

If required, enter a sample name. This name
followed by an incrementing sample number will be
used for naming subsequent results.
Press the down arrow key.

Step 6

Set this option to yes, if you need to measure many
different samples and assign different sample
names. The prompt will, by default, contain the
sample name last entered.
Press the down arrow key.

Step 7

Press ok when all parameters have been
entered.

Parameters

Absorbance

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Step

8

Insert reference sample, press .

Step 9

Insert sample, press .
Repeat Step 9 for all samples.
Step 10

Press to see options

For example: Press 3 to see a graph of the result,
press 3 to return to the digital display.

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Options

(select using key pad numbers)

1. Return to parameters screen (Step 2 above).

2. Export result via selected method.

3. Toggle between digital results screen and
graph results screen.

4. Run standard – unavailable under this mode.

5. Go to Utilities Folder

6. Go to Methods Folder

7. Save method – use the left and right arrows to
select a folder to store in (Favourites/Methods
1-9), press the down arrow and enter name.

8. Sample number – add a prefix to the sample
number and reset the incrementing number to
the desired value, toggle the sample prompt.

9. Auto-print – toggles auto-print on/off.

Exit options by pressing .

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Step 1

Press the Options Key .

Step 2

Press 1 to set parameters.

Step 3

If not already in Transmission mode, use the left
and right arrow keys to choose Transmission
mode.
Press the down arrow key.

Step 4

Use the left and right arrow keys to change the
wavelength, or use the number keypad.
Press the down arrow key.

Step 5

If required enter a sample name. This name
followed by an incrementing sample number will
be used for naming subsequent results.
Press the down arrow key.

Step 6

Set this option to yes, if you need to measure
many different samples and assign different
sample names. The prompt will, by default,
contain the sample name last entered.
Press the down arrow key.

Step 7

Press ok when all parameters have been
entered.

Transmission