biochrom Libra S6 User Manual

Libra S6

User Manual

English

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Declaration of Conformity

This is to certify that the Libra S6 and S6H Visible Spectrophotometers
Part number 80-5000-10 and 80-5000-11

Serial number 88000 onwards

manufactured by Biochrom Ltd. conform to the requirements of the following
Directives-: 73/23/EEC & 89/336/EEC

Standards to which conformity is declared

EN 61 010-1: 2001
Safety requirements for electrical equipment for measurement, control and

laboratory use.

EN 61326: 1998
Electrical equipment for measurement, control and laboratory use – EMC

requirements

Signed: Dated: 26

th

Oct 2004

David Parr
Managin g Direc tor
Biochrom Ltd

Postal address Telephone Telefax
Biochrom Ltd [phone] [phone]

22 Cambridge Science Park
Milton Road e mail: [e-mail] website: http://www.biochrom.co.uk
Cambridge CB4 0FJ
England

Registered in England No: 974213
Registered Office: 22 Cambridge Scie nce Park, Milton Road, Cambridge CB4 4FJ, England

.

Biochrom Ltd

Certificate No. 890333

CONTENTS

Unpacking, Positioning and Installation 1
Essential Safety Notes 1

OPERATION 2

Introduction 2
Using the Instrument 3
Absorbance and % Transmission 4
Absorbance Ratio 5
Cell Density 5
Scan 6
Factor Concentration 7
Standard Curve 8
Kinetics 10
To recall a saved method 11

SET UP 12
ACCESSORIES 13
ERROR MESSAGES 13
OUTPUT OF RESULTS 13

Use with serial printer 13
Use with chart recorder 13

USE WITH PC AND THE GRAFICO PC UTILITY
SOFTWARE 14

Installation 14
Introduction 14
Menu Descriptions 15
Practical Aspects 16

MAINTENANCE 17

After Sales Support 17
Lamp Replacement 17
Cleaning and gen eral care of th e instru men t 18
Changing cell holder or removal for cleaning 18
Changing the brightness of the display 18

SPECIFICATION AND WARRANTY 19

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Issue 01 - 12/2004 Libra S6, English 1

Unpacking, Positioning and Installation

• Inspect the instru ment for any signs of damage caused in tran si t. If any damage is

discovered, inform your supp lier immediately. Check the position of the metal lamp
bracket insid e the lamp access area.

• Ensure your proposed installation site conforms to the environmental conditions for safe

operation:

Indoor use only
Temperature 5°C to 35 °C. No te that if you use the instr ument in a room sub ject to
extremes of temperature change du r ing the day, it may be necessary to recalibrate (b y
switching off and then on again) once thermal equilibrium has been established (2-3
hours).
Maximum relative humidity of 80 % up to 31°C decreasing linearly to 50 % at 40°C

• The instrument must be placed on a hard, flat bench or table that can take its weight (<2

kg) such that air is allowed to circulate freely around the inst rument.

• This equipment must be connected to the power supply with the power cord supplied. It

can be used on 90 - 240V supplies.

• Switch on the instrument via the display after it has been plugged in. The instrument

performs a series of self-diagnostic checks for lamp performanc e, wavelength calibration
and diode array pixels; press F2 to proceed.

If the instru ment h as ju s t b een unpacked or h as be en stored in a co ld environmen t, i t should
be allowed to come to thermal equilibrium for 2-3 hours in the laboratory before switching o n
to prevent calibration failure as a result of internal condensation.

• The cell holder supplied with the instrument accepts standard 10mm pathlength glass or

plastic cells (adap t er s are avai lable to convert it to accept 10, 12 and 16mm diameter test
tubes). It can be removed for cleaning if spillages occur by undoing the screws that hold
it or it can be flushed through with water in situ.

• If the instrument has a heated cell holder option and it is on, allow 10 minutes for it to

come to thermal equilibrium. This cell holder cannot be removed.

If this equipment is used in a manner not specified or in environmental conditions not
appropriate for safe operation, the protection provided by the equipment may be impaired and
instrument w a rranty withdrawn.

Essential Safety Notes

There are a number of warning labels and symbols on your instrument. These are
there to inform you where potential danger exists or particular caution is required.
Before commencing installation, please take time to familiarise yourself with these
symbols and their meaning.

Caution (refer to accompanying documents).
Background colour yellow, symbol and outline black.

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2 Libra S6, English Issue 01 – 12/2004

OPERATION

Introduction

Your spectrophotometer is a simple-to-use, microprocessor controlled instrument. It is a
diode array product (1024 pixels), has no moving parts and scans very quickly.

After switch on, calibrati on and press in g F2 to proceed the home page is shown offer ing the
choice of

• Repeat last op er ation

• Make a measurement

• Set up instrume nt

“Repeat last operation” returns the user to the last screen displayed when the instrument was
switched off, and provides a short cut to the last test that was performed.

Within “Make a measurement” your spectrophotometer has facilities for:

• measurement of absorba n ce, % transmission, r atio and conce n tr at ion values

• cell culture optical density measurements at 60 0nm

• entry of a multi point stan dard curve in memory

• output of wavelength scan to display

• output of kinetics assay to display

• application of a factor to an absorbance change over a specified time interval for an

enzymatic determination (reaction rate)

• storage of up to 99 user defined methods

Within “Set up instrument” your spectrophotometer can be set up to

• select the display language option (English, French, German, Spanish, Italian)

• link via a serial lead to either a serial printer for hardcopy ou tput or to a PC for

download of results to spreadsheet

• link via a converte r lead to chart record e r

• set the da te for print outs

The instrument is supplied with Grafico PC utility - on the accompanying CD - and a serial
lead. These provide the user with th e means to cap t ure, print an d s tore data from the
instrument to a PC. Specifically it

• produces a printable graphical plot of the scan, in Abs

• logs date, time and serial number with any output from the instrument

• produces a results log in order to store, tabulate and subsequently print output from

the instrument

• enables expor t of the outpu t from the instrument to Excel as a text file

A tutorial on UV/V isib l e spe ctrophotometry is included as part of the Grafico software.

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Issue 01 - 12/2004 Libra S6, English 3

Using the Instrument

The back-lit liquid crystal display is very easy to navigate around using the function / select
and arrow keys on the hard wearing, spill proof membrane keypad.

Keypad

F1, F2, F3, F4

The function select / entry soft keys on the keypad are situated next to
the corresponding option on the display, and are used to select an
appropriate mode

3456

When a parameter within a mode needs selecting or changing (as
indicated by highlighted text on the display), the four arrow ke ys
(3456) are used in conjunction with the function keys to make that
selection or chan ge. Use F4 to implement change, followed by 34 to
choose between options indicated, and 56 to enter alphanumeric
characters (for example in th e selection of a wavelength o r en try of a
method title). Then u se F 4 to acce p t th e ch an ge made.

to escape or stop making measurements

0A/100%T to set reference to 0.000AU or 100%T on a reference solution at the

current wavelength in the mode selected , or to do a referen c e scan if in
scan mode

to start making measurements

Display

The following symbols will appear in bottom right hand corner and mean

the following:
◊ Use 3456 to select option
R T Ready to set reference or r u n sample

Note that the light beam shines from LEFT to RIGHT through the cell chamber; ensure the
cell is inserted in the corre ct ali gn ment. The optical height is 15mm, and the minimum
volume that can be used is approx. 700µl in a semi-micro cell.