biochrom Libra S21, Libra S22 User Manual

Libra S21 and S22

User Manual

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Declaration of Conformity

This is to certify that the Libra S21 Visible and Libra 22 UV/Visible

Spectrophotometers
Part number 80-2115-25 and 80-2115-20
Serial number 81000 onwards

manufactured by Biochrom Ltd. conforms to the requirements of the following
Directives-: 73/23/EEC & 89/336/EEC

Standards to which conformity is declared

EN 61 010-1: 2001
Safety requirements for electrical equipment for measurement, control and

laboratory use.

EN 61326: 1998
Electrical equipment for measu rement, control and laboratory use – EMC

requirements

Signed: Dated: 23

nd

October 2002

David Parr
Managi ng Direct or
Biochrom Ltd

Postal address Telephone Telefax
Biochrom Ltd [phone] [phone]

22 Cambridge Science Park
Milton Road e mail: enquiries@bio chr om.co.uk website: http://www.biochrom.co.uk
Cambridge CB4 0FJ
England

Registered in England No: 3526954
Registered Office: 22 Cambridge Science Park, Milton Road, Cambridge CB4 0FJ, England

.

Biochrom Ltd

Certificat e No. 890333

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Issue 02 - 04/2004 Libra S21/S22, English 1

CONTENTS
UNPACKING, POSITIONING AND INSTALLATION 2

Essential Safety Notes 3

OPERATION 4

Introduction 4
Keypad and display 5
Basic Modes (1) 6

Absorbance (1.1) 6
% Transmission (1.2) 6
Factor Concentration (1.3) 6
Ratio (1.4) 7

Applications (2) 8

Wavescan (2.1) 8
Simple Kinetics (2.2) 9
Reaction Rate (2.3) 10
Standard Curve (2.4) 11
Multiwave and Equation Entry (2.5) 12

Methods A (4), B (5) and C (6) 13
System Utilities 14
Output to Printer 16

Seiko DPU-414 (1) 16
Epson FX-80+ / Epson 9 pin (2) 16
Text printer (no graphics) (3) 16
HP PCL 3 (4) 16
Epson 24 pin (ESC P) (5) 16

Download to Spreadsheet 17
Messages 17

ACCESSORIES 18

Multiple Cell Holder Accessories 18
Single Cell Holder Accessories 19
Other Accessories, consumables etc 20
Acquire Applications Software 21

MAINTENANCE 22

After Sales Support 22
Fuse Replacement 22
Cleaning and General Care 23

APPENDIX 24

Text entry 24
Good Labor a to r y Pr ac ti ce 25
Least squares regression analysis and linearity 26

SPECIFICATION AND WARRANTY 27

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2 Libra S21/S22, English Issue 02 - 04/2004

Unpacking, Positioning and Installation

• Inspect the instrument for any signs of damage caused in transit. If any damage

is discovered, inform your supplier immediately.

• Ensure your proposed installation site conforms to the environmental conditions

for safe operation:

Indoor use only
Temperature 10°C to 40°C
Maximum relative humidity of 80 % up to 31°C decreasing linearly to 50 %
at 40°C

• The instrument must be pl aced on a hard flat surface, fo r example a laboratory

bench or table, which can take its weight (13 kg) such that air is allowed to
circulate freely around the instrument.

• Ensure that the cooling fan inlets and outlets are not obstructed; position at least

2 inches from the wall.

• This equipment must be connected to the power supply with the power cord

supplied and must be earthed (grounded)

. It can be used on 90 - 240V supplies.

• Switch on the instrument and check that the display works (see Operation).

• To enter laboratory name, operator name, instrument asset number details, and

current date/time, refer to System Utilities.

If this equipment is used in a manner not specified or in environmental conditions
not appropriate for safe operation, the protection provided by the equipment may be
impaired and instrument warranty withdrawn.

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Issue 02 - 04/2004 Libra S21/S22, English 3

Essential Safety Notes

There are a number of warning labels and s ymbols on your instrument. These are
there to inform you where potential danger exists or particular caution is required.
Before commencing installation, please take time to familiarise yourself with these
symbols and their meaning.

Caution (refer to accompanying documents).
Background colour is yellow, symbol and outline are black.

UV RADIATION UV RADIATION IS HARMFUL TO YOUR EYES
HOT If power is restored with this cover removed,

eye protection must be worn

Accessories

• Care should be taken when handling all heated accessories.

• Ensure that the cell compartment lid is closed when operating cell changers and

the sipper.

• It is essential that the baseplate plug supplied with single cell accessories is

fitted to optimise air flow and to prevent light ingress.

WARNING

WARNING

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4 Libra S21/S22, English Issue 02 - 04/2004

OPERATION
Introduction

Your spectrophotometer is a stand alone, simple-to-use instrument with a high­resolution liquid cr ystal displa y (LCD), and a compr ehensive ra nge of
spectrophotometry measurements can be undertaken.

It works on the basis of light from the xenon lamp being directed by a fixed mirror
through the monochromator inlet slit. This passes through one of several (dependent
on wavelength selected) filters mounted on filter quadrant: the filtered light is then
directed onto the holographic grating which produces light of the selected
wavelength. The light then leaves the monochromator via the exit slit, and mirrors
focus and direct the light into the sample compartment. This passes through your
cell, containing the sample of interest, and then a defocusing lens to a solid state
detector unit. The resulting signal is then filtered and displayed.

Your spectrophotometer has the following capabilities:

• Basic Modes for

- Absorbance

- % Transmission

- Factor Concentrat ion

- Absorbance Ratio

• Application Mod e s for

- Wavescan (Wavelength Scanning )

- Simple Kinetics

- Reaction Rate

- Standard Curve

- Multiple Wavelength (Multi Wavelength Equation Entry)

• 18 user defined me thods, in 3 groups of 6

- Methods A, B, C

• Print results from the instrument display in graphical format

• Download of results directly to Excel for manipulation and archiving, via a

serial interface lead to a PC

• Self test diagnostics for GLP purposes

A range of accessories further enhances the capability of the instrument.
The home page provides access to user modes, system utilities and accessory
identification and set-up.

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Issue 02 - 04/2004 Libra S21/S22, English 5

Keypad and display

Press the soft key on the keypad directly below the corresponding option on the
display (F1, F2 and F3) to select that option. For example, on the home page
(above):

• press F1 to take you to System Utilities

• press F2 to identify the type of cell changer / holder that has been fitted

• press F3 to toggle the display back-light on/off (display contrast can be

changed within System (F1)

Press:

• to print result if auto-print is off, or to r e -print result if auto-print is on

← to back space in order to correct text and characters in appropriate boxes

to start making measurements and print results (green run key)

to stop making measurements or entering parameters and return to the home

page; use as an “escape mechanism” (red stop key)

Press the corresponding number on the keypad to enter the user mode choices; for
example 1 followed by 1 is Absorbance mode, whereas 2 followed by 4 is Standard
Curve Mode.

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6 Libra S21/S22, English Issue 02 - 04/2004

Basic Modes (1)

Absorbance (1.1 )

Absorbance mode measures the amount of light that has passed through a sample
relative to a blank (this can be air). The procedure is as follows:

• Enter approp ria te wavelength and press OK (F3)

• Insert reference and press green run key. The cell changer, if fitted,

automatically moves to position 2 and displays the result for the reference
measurement (0.000)

• Xenon lamp based instruments are “press to read”, whereas deuterium /

tungsten lamp instruments measure continuously. Thus to monitor sample
stabilisation, the simple kinetics mode must be used

• This reference value is used for subsequent samples until changed

• Insert samples as required and press (repeat as necessary)

• To go back and change the wavelength press Method (F1)

% Transmission (1.2)

Transmission mode measures the amount of light that has passed through a sample
relative to a blank (this can be air), but displays the result as a percentage. The
procedure is as follows:

• Enter approp ria te wavelength and press OK (F3)

• Insert reference and press green run key

• This reference value is used for subsequent samples until changed

• Insert samples as required and press (repeat as necessary)

• To go back and change the wavelength press Method (F1)

Factor Concentration (1.3)

Concentration mode is used when a conversion factor is known, and converts the
absorbance measurement for a sample at a specific wavelength into a concentration,
by a simple multiplication of absorbance x factor. The procedure is as follows:

• Enter approp ria te wavelength and press OK (F3)

• Enter known factor (range 0.01-9999) and press OK (F3)

• To enter a negative factor press – (F1); the reference should have a higher

absorbance than the samples

• Insert reference and press green run key

• This reference value is used for subsequent samples until changed

• Insert samples as required and press (repeat as necessary)

• To go back and change the wavelength or factor press Method (F1)