biochrom EZ Read 400 User Manual

Biochrom EZ Read 400 User’s Manual Version 2.1 1

Biochrom EZ Read 400

Microplate Reader

User’s Manual

Biochrom Ltd

22 Cambridge Science Park,

Milton Rd, Cambridge CB4 0FJ UK

Tel: [phone]

Fax: [phone]

Email: [e-mail]

Biochrom US

84 October Hill Road,

Holliston, MA 01746-1388 USA

Tel: (Toll free): 877-BIO-CHROM (877-246-2476)

Fax: [phone]

Email: [e-mail]

Biochrom EZ Read 400 User’s Manual Version 2.1 2

Table of Contents

Table of Contents ............................................................................................................. 2

1 Declaration of Conformity for Biochrom Manufactured Products ............................... 4

2 Introduction and Intended Use .................................................................................. 5

2.1 What to expect from the user’s manual ..................................................................... 5

2.2 Instrument Use ............................................................................................................ 5

3 Safety Information ..................................................................................................... 6

3.1 Electrical Safety ........................................................................................................... 6

3.2 Chemical and Biological Safety ................................................................................... 6

3.3 Safety issues regarding the use of a PC with the instrument: .................................... 7

3.4 Safety concerns regarding moving parts: ................................................................... 7

3.5 Instrument Inputs and Outputs .................................................................................. 8

3.6 Instrument Validation ................................................................................................. 9

3.7 Table 1: Rated Operating Conditions .......................................................................... 9

3.8 Unpacking the EZ Read 400 ....................................................................................... 10

3.9 Powering up the EZ Read 400 Microplate Reader .................................................... 10

3.10 Cleaning and Disinfecting the Instrument ................................................................ 11

3.11 Emergency Instructions ............................................... Error! Bookmark not defined.

3.12 Safe Disposal of the Instrument ................................................................................ 11

3.13 Regular Maintenance ................................................................................................ 12

4 Software Installation and Use .................................................................................. 13

4.1 Software Installation ................................................................................................. 13

Biochrom EZ Read 400 User’s Manual Version 2.1 3

4.2 Endpoint Measurements ........................................................................................... 14

4.3 Kinetic Measurements .............................................................................................. 14

4.4 Multiwavelength Measurements .............................................................................. 15

4.5 Additional Features ................................................................................................... 15

4.6 Calibrating the Instrument ........................................................................................ 16

5 General features ...................................................................................................... 17

5.1 Measurement modes ................................................................................................ 17

5.2 Integrated quality control ......................................................................................... 17

5.3 Scope of supply ......................................................................................................... 17

5.4 Liability ...................................................................................................................... 17

6 Ordering Information and Accessories ..................................................................... 18

7 Contact Information ................................................................................................ 19

7.1 Table 3 Contact Information ..................................................................................... 19

8 Appendix: ................................................................................................................ 20

8.1 Helpful Hints for Successful Microplate Assays ........................................................ 20

8.2 Quick Start Guide ...................................................................................................... 21

8.3 Troubleshooting and Frequently Asked Questions ................................................... 23

Biochrom EZ Read 400 User’s Manual Version 2.1 4

1 Declaration of Conformity for Biochrom Manufactured Products

This is to certify that the following Biochrom manufactured products conform to the
requirements of the following Directives:

EZ Read 400 Elisa (80-4001-40), EZ Read 400 Research (80-4001-41) and EZ Read 400 Flexi
(80-4001-42)

2006/95/EC Low voltage equipment safety directive
2004/108/EC EMC directive
2002/96/EC & 2003/108/EC EC Directive on Waste Electrical and Electronic Equipment
(WEEE)
2002/95/EC ROHS directive
98/79/EC In Vitro Diagnostic Medical Devices Directive

Standards to which conformity is declared, where relevant, are as follows

EN61010-1:2010 Safety requirements for electrical equipment for

measurement, control and laboratory use. General
requirements

EN61326-1:2006 Electrical equipment for measurement, control and

laboratory use -EMC Requirements

EN 12100-1,2:2003 (+A1:2009) Safety of machinery – Basic concepts, general principles for

design

EN 14121-1:2007 Safety of machinery, Risk assessment

For further information, including unpacking, positioning and installation of the products,
please refer to the user manual.

Signed:

Brian Clarkstone

Technical Director

Biochrom Ltd

Biochrom EZ Read 400 User’s Manual Version 2.1 5

2 Introduction and Intended Use

2.1 What to expect from the user’s manual

This manual will allow the user to:

 Understand who should operate the instrument and why would be used.
 Understand all the important safety information when operating the instrument.
 How to safely unpack and install the instrument.
 Scope of supply
 Technical specifications
 A diagram of the outside of the instrument.
 A technical explanation of the workings of the instrument.
 How to use the instrument and the reader control software to perform basic

measurements.

 Optional software and accessories that may be used with the instrument.
 Helpful hints for obtaining the best measurements using the instrument.

2.2 Instrument Use

The Biochrom EZ Read 400 Microplate Reader is used for microplate-based applications
requiring endpoint or kinetic absorbance measurements from 400-750 nm in optically clear,
flat-bottomed 96-well plates.

Only trained laboratory personnel should operate the Biochrom EZ Read 400 Microplate
Reader. The Biochrom EZ Read 400 Microplate Reader is intended for general laboratory and
research use only.

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3 Safety Information

This symbol indicates that a potential for safety risk. Please carefully review
the safety information provided below. If the instrument is used in a manner
not specified by the manufacturer, the protection provided by the equipment
may be impaired.

3.1 Electrical Safety

To prevent electrically related injuries and property damage, properly inspect all electrical
equipment prior to use and immediately report any electrical deficiencies. Contact a Biochrom
service representative for instrument service requiring the removal of covers or panels.

3.1.1 Please note these particular electrical safety concerns:

 Voltages dangerous to human life are present in this device. Before removing any

covers or panels disconnect the power supply from the power source.

 Ensure that only the power supply supplied with the unit is used.
 The power supply may only be inserted in a socket outlet provided with a protective

ground (earth) contact. Use only the supplied mains lead cords with the instrument.

 The mains cord should be positioned in a way that enables disconnection in the

event of an emergency

 Extensions cords should not be used with the instruments.
 Only a qualified service engineer should adjust or repair the instrument.
 Use the equipment only in the intended manner.

3.2 Chemical and Biological Safety

Normal operation of the Biochrom EZ Read 400 Microplate Reader may involve the use of
materials that are toxic, flammable, infectious or otherwise biologically harmful. This general
purpose laboratory equipment could be used with IVD samples and as such can be considered
to be IVD medical equipment.

This symbol denotes the potential for bio-hazardous material on or around
the instrument. When using such materials, observe the following
precautions:

Biochrom EZ Read 400 User’s Manual Version 2.1 7

 Handle infectious samples or dangerous materials in accordance with good

laboratory practices.

 Wear protective clothing when using the instrument including but not limited to

laboratory gloves, laboratory coat and safety glasses

 Observe all cautionary information printed on the original solutions containers prior

to their use.

 Dispose of all waste solutions safely and in accordance with the policies and

practices of your facility.

 Operate the Biochrom Anthos Microplate Reader in accordance with the instructions

outlined in this manual.

 Use appropriate precautions when using pathological, toxic, or radioactive materials.

Consult the MSDS of all materials used in the laboratory.

 Use an appropriately contained environment when using hazardous materials.
 Wash your hands thoroughly after handling test fluids. If equipment has been in

contact with hazardous substances, it must be disinfected prior to shipment in
accordance with the effective provisions. (See 6.4 Decontamination Instructions).

3.3 Safety issues regarding the use of a PC with the instrument:

This equipment may be connected and controlled from a PC. To preserve the integrity of the
measuring equipment it is essential that the attached PC itself conforms to basic safety and

EMC standards and is set up in accordance with the manufacturers’ instructions. If in doubt

consult the information that came with your PC. In common with all computer operation the
following safety precautions are advised.

 To reduce the chance of eye strain, set up the PC display with the correct viewing

position, free from glare and with appropriate brightness and contrast settings.

 To reduce the chance of cross contamination from biological samples, use

appropriate personnel protection measures and disinfectant wipes on keyboard and
mouse.

3.4 Safety concerns regarding moving parts:

 Do not touch the plate or the instrument during movement of the plate transport

(risk of injury to the user or damage to the instrument).

 Keep the area around the microplate reader clear of clutter

Biochrom EZ Read 400 User’s Manual Version 2.1 8

Safety Symbols

Place plate here prior to measurement.

Power Supply Input

Safety Symbols

3.5 Instrument Inputs and Outputs

Note the presence of Biohazardous and General safety signs present on the instrument.
Review the safety information available in sections 2.1 and 2.2.

USB A to B cable Input

Figure 1 Front of the EZRead 400 Microplate Reader

Figure 2 Back of the EZREAD 400 Microplate Reader

On/Off Switch