BINDER KB 23 User Manual

Model

Art. No.

Operating Manual

APT.line™ KB (E3.1), KB (E5.1)
Cooling Incubators

with compressor technology and program control

KB 23 (E3.1) 9020-0112, 9120-0112
KB 23-UL (E3.1) 9020-0113, 9120-0113
KB 53 (E3.1) 9020-0243, 9120-0243
KB 53-UL (E3.1) 9020-0252, 9120-0252
KB 115 (E3.1) 9020-0242, 9120-0242
KB 115-UL (E3.1) 9020-0253, 9120-0253
KB 240 (E5.1) 9020-0241, 9120-0241
KB 240-UL (E5.1) 9020-0254, 9120-0254
KB 400 (E5.1) 9020-0178, 9120-0178
KB 400-UL (E5.1) 9020-0179, 9120-0179
KB 720 (E5.1) 9020-0111, 9120-0111
KB 720-UL (E5.1) 9020-0167, 9120-0167

BINDER GmbH

Address Post office box 102
D-78502 Tuttlingen
Tel. [phone]
Fax [phone]
Internet http://www.binder-world.com
E-mail [e-mail]
Service Hotline [phone]
Service Fax [phone]
Service E-Mail [e-mail]
Service Hotline USA [phone] or [phone] x3
Service Hotline Asia Pacific [phone] or [phone]
Service Hotline Russia and CIS [phone]

Issue 11/2014 Art. No. 7001-0177

EG – KONFORMITÄTSERKLÄRUNG

EC – declaration of conformity

EC - DECLARATION OF CONFORMITY
CE - DECLARATION DE CONFORMITE

Anbieter / Supplier / Fournisseur:

Anschrift / Address / Adresse:

Produkt / Product / Produit:

Typenbezeichnung / Type / Type:

Die oben beschriebenen Produkte sind konform mit folgenden EG-Richtlinien:
The products described above are in conformity with the following EC guidelines:
Les produits décrits ci-dessus sont conformes aux directives CE suivantes:

Niederspannungsrichtlinie
2006/95/EG

Low voltage directive
2006/95/EC

Directive basse tension
2006/95/CE

BINDER GmbH

Im Mittleren Ösch 5, D-78532 Tuttlingen

Kühlinkubatoren mit Kompressortechnologie
Cooling incubators with compressor technology
Incubateurs réfrigérés avec technologie de compresseur

KB 23, KB 53, KB 115, KB 240, KB 400, KB 720

Richtlinie 2006/95/EG des Europäischen Parlaments und des
Rates vom 12. Dezember 2006 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten betreffend elektrische
Betriebsmittel zur Verwendung innerhalb bestimmter
Spannungsgrenzen

Council Directive 2006/95/EC of 12 December 2006 on the
harmonization of the laws of Member States relating to electrical
equipment designed for use within certain voltage limits

Directive 2006/95/CE du Parlement Européen et du Conseil du 12
décembre 2006 concernant le rapprochement des législations des
États membres relatives au matériel électrique destiné à être
employé dans certaines limites de tension

EMV-Richtlinie
2004/108/EG

EMC Directive
2004/108/EC

Directive CEM
2004/108/CE

Die oben beschriebenen Produkte tragen entsprechend die Kennzeichnung CE.
The products described above, corresponding to this, bear the CE-mark.
Les produits décrits ci-dessus, en correspondance, portent l’indication CE.

Richtlinie 2004/108/EG des Europäischen Parlaments und des
Rates vom 15. Dezember 2004 zur Angleichung der
Rechtsvorschriften der Mitgliedstaaten über die
elektromagnetische Verträglichkeit und zur Aufhebung der
Richtlinie 89/336/EWG.

Directive 2004/108/EC of the European Parliament and of the
Council of 15 December 2004 on the approximation of the laws of
the Member States relating to electromagnetic compatibility and
repealing Directive 98/336/EEC.

Directive 2004/108/CE du Parlement Européen et du Conseil du
15 décembre 2004 relative au rapprochement des législations des
États membres concernant la compatibilité électromagnétique et
abrogeant le directive 98/336/CEE.

1 / 3

KB (E3.1 + E5.1) 11/2014 page 2/100

Die oben beschriebenen Produkte sind konform mit folgenden harmonisierten Normen:
The products described above are in conformity with the following harmonized standards:
Les produits décrits ci-dessus sont conformes aux normes harmonisées suivantes:

Sicherheit / safety / sécurité:

EN 61010-1:2010 Sicherheitsbestimmungen für elektrische Mess-, Steuer-, Regel- und

Laborgeräte – Teil 1: Allgemeine Anforderungen (DIN EN 61010-1:2011,
VDE 411-1:2011)

Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 1: General requirements (IEC 61010-1:2010,
BS EN 61010-1:2010)

Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 1: Prescriptions générales (CEI
61010-1:2010, NF EN 61010:2011)

EN 61010-2-010:2003 Sicherheitsbestimmungen für elektrische Meß-, Steuer-, Regel- und

Laborgeräte – Teil 2-010: Besondere Anforderungen an Laborgeräte für
das Erhitzen von Stoffen (DIN EN 61010-2-010:2004)

Safety requirements for electrical equipment for measurement, control,
and laboratory use – Part 2-010: Particular requirements for laboratory
equipment for the heating of materials (IEC 61010-2-10:2005, BS EN
61010-2-10:2003)

Règles de sécurité pour appareils électriques de mesurage, de
régulation et de laboratoire – Partie 2-010 : Prescriptions particulières
pour appareils de laboratoire utilisés pour l’échauffement des matières
(CEI 61010-2-10:2003, NF EN 61010-2-10:2005)

EMV / EMC / CEM:

EN 61326-1:2013 Elektrische Mess-, Steuer-, Regel- und Laborgeräte - EMV-

Anforderungen - Teil 1: Allgemeine Anforderungen (DIN EN 61326­1:2013, VDE 0813-20-1:2013)

Electrical equipment for measurement, control and laboratory use ­EMC requirements - Part 1: General requirements (IEC 61326-1:2012,
BS EN 61326-1:2013)

Matériel électrique de mesure, de commande et de laboratoire ­Exigences relatives à la CEM - Partie 1: Exigences générales (CEI
61326-1:2012, NF EN 61326-1:2013)

D-78532 Tuttlingen, 06.10.2014
BINDER GmbH

P. M. Binder
Geschäftsführender Gesellschafter

Managing Director
Directeur général

J. Bollaender
Leiter F & E

Director R & D
Chef de service R&D

2 / 2

KB (E3.1 + E5.1) 11/2014 page 3/100

Product registration

KB (E3.1 + E5.1) 11/2014 page 4/100

Contents

EC – declaration of conformity ...................................................................................................................... 2

Product registration ....................................................................................................................................... 4

1. SAFETY .................................................................................................................. 7

1.1 Legal considerations ........................................................................................................................... 7

1.2 Structure of the safety instructions ...................................................................................................... 7

1.2.1 Signal word panel ...................................................................................................................... 7

1.2.2 Safety alert symbol .................................................................................................................... 8

1.2.3 Pictograms ................................................................................................................................ 8

1.2.4 Word message panel structure ................................................................................................. 9

1.3 Localization / position of safety labels on the unit ............................................................................... 9

1.4 Type plate .......................................................................................................................................... 10

1.5 General safety instructions on installing and operating the cooling incubator ................................... 12

1.6 Intended use ...................................................................................................................................... 14

2. UNIT DESCRIPTION ............................................................................................ 15

2.1 Unit overview ..................................................................................................................................... 16

2.2 Control panel ..................................................................................................................................... 17

2.3 KB (E5.1) 240 / 400 / 720: Lateral control panel at the right side (option) ........................................ 18

2.4 KB (E5.1) 240 / 400 / 720: Lateral control panel at the left side (option) ........................................... 19

2.5 Unit rear ............................................................................................................................................. 20

3. COMPLETENESS OF DELIVERY, TRANSPORTATION, STORAGE, AND

INSTALLATION .................................................................................................... 20

3.1 Unpacking, and checking equipment and completeness of delivery................................................. 20

3.2 Guidelines for safe lifting and transportation ..................................................................................... 21

3.3 Storage .............................................................................................................................................. 21

3.4 Location of installation and ambient conditions ................................................................................. 22

4. INSTALLATION OF THE EQUIPMENT ............................................................... 23

4.1 Spacers for wall distance (KB 240, 400, 720) ................................................................................... 23

4.2 Electrical connection ......................................................................................................................... 24

5. START UP ............................................................................................................ 25

5.1 Settings at the RD3 program controller ............................................................................................. 25

5.2 General indications ............................................................................................................................ 26

6. FIXED VALUE ENTRY MODE ............................................................................. 27

7. WEEK PROGRAM EDITOR ................................................................................. 29

7.1 Program table template for Week program Editor ............................................................................ 31

8. PROGRAM EDITOR ............................................................................................. 32

8.1 Selecting between set-point ramp and set-point step ....................................................................... 32

8.1.1 Programming with setting “Ramp” (default setting) ................................................................ 32

8.1.2 Programming with setting “step” ............................................................................................. 34

8.1.3 General notes on programming of temperature transitions .................................................... 35

8.2 Set-point entry for program operation ............................................................................................... 35

8.3 Program table template for Program Editor ...................................................................................... 39

8.4 Deleting a program section ............................................................................................................... 40

9. PROGRAM START LEVEL .................................................................................. 41

10. USER LEVEL ....................................................................................................... 44

11. PROGRAMMING EXAMPLE OF THE WEEK PROGRAM EDITOR .................... 51

11.1 Desired time function ........................................................................................................................ 51

11.2 Proceeding overview ......................................................................................................................... 51

KB (E3.1 + E5.1) 11/2014 page 5/100

11.3 Proceeding in detail ........................................................................................................................... 52

12. PROGRAMMING EXAMPLE OF THE PROGRAM EDITOR ................................ 58

12.1 Desired time function ........................................................................................................................ 58

12.2 Proceeding overview ......................................................................................................................... 58

12.3 Proceeding in detail ........................................................................................................................... 59

13. PERFORMANCE IN CASE OF FAILURES .......................................................... 65

13.1 Performance after a power failure ..................................................................................................... 65

13.2 Alarm messages ............................................................................................................................... 65

14. TEMPERATURE SAFETY DEVICES ................................................................... 65

14.1 Over temperature protective device (class 1) ................................................................................... 65

14.2 Safety controller (temperature safety device class 3.1) .................................................................... 65

14.3 Temperature safety device class 3.3 (option) ................................................................................... 66

14.3.1 Temperature safety device class 3.1 ...................................................................................... 68

14.3.2 Temperature safety device class 3.2 ...................................................................................... 69

15. DEFROSTING DURING REFRIGERATING OPERATION ................................... 70

16. OPTIONS .............................................................................................................. 71

16.1 Communication software APT-COM™ 3 DataControlSystem (option) ............................................. 71

16.2 Ethernet interface (option for KB 240, 400, 720) ............................................................................... 71

16.3 Data logger kit (option) ...................................................................................................................... 71

16.4 Analog output for temperature (option) ............................................................................................. 72

16.5 Water protected internal socket (option from size 53 on – not for KB-UL) ....................................... 72

16.6 Zero-voltage relay outputs via operation lines (option from size 53 on) ............................................ 73

16.7 Additional flexible Pt 100 temperature sensor (option for KB 53, 115) ............................................. 74

16.8 Additional flexible Pt 100 temperature sensor (option for KB 240, 400, 720) ................................... 74

17. MAINTENANCE, CLEANING, AND SERVICE ..................................................... 75

17.1 Maintenance intervals, service .......................................................................................................... 75

17.2 Cleaning and decontamination .......................................................................................................... 76

17.2.1 Cleaning .................................................................................................................................. 76

17.2.2 Decontamination ..................................................................................................................... 77

17.3 Sending the unit back to BINDER GmbH .......................................................................................... 78

18. DISPOSAL ............................................................................................................ 79

18.1 Disposal of the transport packing ...................................................................................................... 79

18.2 Decommissioning .............................................................................................................................. 79

18.3 Disposal of the unit in the Federal Republic of Germany .................................................................. 79

18.4 Disposal of the unit in the member states of the EC except for the Federal Republic of Germany .. 80

18.5 Disposal of the unit in non-member states of the EC ........................................................................ 82

19. TROUBLESHOOTING ......................................................................................... 83

20. TECHNICAL DESCRIPTION ................................................................................ 85

20.1 Factory calibration and adjustment ................................................................................................... 85

20.2 Over current protection...................................................................................................................... 85

20.3 Definition of usable volume ............................................................................................................... 85

20.4 KB (E3.1) technical data .................................................................................................................... 86

20.5 KB (E5.1) technical data .................................................................................................................... 88

20.6 Equipment and Options ..................................................................................................................... 90

20.7 Spare parts and accessories ............................................................................................................. 91

20.8 Dimensions KB 240 ........................................................................................................................... 92

20.9 Dimensions KB 400 ........................................................................................................................... 93

20.10 Dimensions KB 720 ........................................................................................................................... 94

21. CONTAMINATION CLEARANCE CERTIFICATE ................................................ 95

21.1 For units located outside North America and Central America ......................................................... 95

21.2 For units in North America and Central America ............................................................................... 98

KB (E3.1 + E5.1) 11/2014 page 6/100

Dear customer,

For the correct operation of the cooling incubator KB, it is important that you read this operating manual
completely and carefully and observe all instructions as indicated. Observe the national regulations on
minimum age of laboratory personnel. Failure to read, understand and follow the instructions may result in
personal injury. It can also lead to damage to the unit and/or poor equipment performance.

1. Safety

This operating manual is part of the components of delivery. Always keep it handy for reference. The
device should only be operated by laboratory personnel especially trained for this purpose and familiar
with all precautionary measures required for working in a laboratory. To avoid injuries and damage
observe the safety instructions of the operating manual.

WARNING

Failure to observe the safety instructions.

Serious injuries and unit damage.

 Observe the safety instructions in this operating manual.
 Carefully read the complete operating instructions of the cooling incubator KB.

1.1 Legal considerations

This operating manual is for informational purposes only. It contains information for installing, start-up,
operation and maintenance of the product. Note: the contents and the product described are subject to
change without notice.

Understanding and observing the instructions in this operating manual are prerequisites for hazard-free
use and safety during operation and maintenance. In no event shall BINDER be held liable for any
damages, direct or incidental arising out of or related to the use of this manual.

This operating manual cannot cover all conceivable applications. If you would like additional information,
or if special problems arise that are not sufficiently addressed in this manual, please ask your dealer or
contact us directly by phone at the number located on page one of this manual

Furthermore, we emphasize that the contents of this operating manual are not part of an earlier or existing
agreement, description, or legal relationship, nor do they modify such a relationship. All obligations on the
part of BINDER derive from the respective purchase contract, which also contains the entire and
exclusively valid statement of warranty administration. The statements in this manual neither augment nor
restrict the contractual warranty provisions.

1.2 Structure of the safety instructions

In this operating manual, the following safety definitions and symbols indicate dangerous situations
following the harmonization of ISO 3864-2 and ANSI Z535.6.

1.2.1 Signal word panel

Depending on the probability of serious consequences, potential dangers are identified with a signal word,
the corresponding safety color, and if appropriate, the safety alert symbol.

DANGER

Indicates an imminently hazardous situation that, if not avoided, will result in death or serious
(irreversible) injury.

KB (E3.1 + E5.1) 11/2014 page 7/100

Warning signs

Electrical hazard

Hot surface

Explosive atmosphere

Stability hazard

or chemical burns

Pollution Hazard

Mandatory action signs

plug

assistance

WARNING

Indicates a potentially hazardous situation which, if not avoided, could result in death or serious
(irreversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in moderate or minor
(reversible) injury.

CAUTION

Indicates a potentially hazardous situation which, if not avoided, may result in damage to the product
and/or its functions or of a property in its proximity.

1.2.2 Safety alert symbol

Use of the safety alert symbol indicates a risk of injury.
Observe all measures that are marked with the safety alert symbol in order to avoid death or

injury.

1.2.3 Pictograms

Lifting hazard

Risk of corrosion and /

Harmful substances

Biohazard

Mandatory regulation

Lift with mechanical

KB (E3.1 + E5.1) 11/2014 page 8/100

Read operating

instructions

Environment protection

Disconnect the power

Wear protective gloves

Lift with several persons

Wear safety goggles

Prohibition signs

water

Pictograms (Warning signs)

Service label

KB with optional interior socket: below the

Do NOT touch

Do NOT spray with

Information to be observed in order to ensure optimum function of the product.

Do NOT climb

1.2.4 Word message panel structure

Type / cause of hazard.

Possible consequences.

∅ Instruction how to avoid the hazard: prohibition

 Instruction how to avoid the hazard: mandatory action

Observe all other notes and information not necessarily emphasized in the same way, in order to avoid
disruptions that could result in direct or indirect injury or property damage.

1.3 Localization / position of safety labels on the unit

The following labels are located on the unit:

Hot surface

• Inner glass door above the glass door
handle

• Outer unit door (KB-UL only)

• On unit rear next to the exhaust duct

Read operating manual

• UL units: outer unit door

•

interior socket

KB (E3.1 + E5.1) 11/2014 page 9/100

Unit front KB

Unit front KB-UL

Nominal temperature

100°C

1,20 kW

Max. operating pressure 15 bar

212°F

200-240 V 1 N ~

R 134A - 0,35 kg

Temp. safety device

IP 20
DIN 12880

50 Hz

gases

Class

3.1 covered by the Kyoto Protocol

Art. No.

9020-0241

US PATS 4585923 / 5222612 / 5309981

Built

2014

Cooling incubator

D 78532 Tuttlingen / Germany

Internet: www.binder-world.com

KB 240 Serial No. 00-00000

Figure 1: Position of labels on the unit (example)

Keep safety labels complete and legible.

Replace safety labels that are no longer legible. Contact BINDER Service for these replacements.

1.4 Type plate

The type plate of KB 23, 53, 115 (E3.1) is located behind the outer door, bottom left-hand.
The type plate of KB 240, 400, 720 (E5.1) sticks to the left side of the unit, bottom right-hand.

Enclosure protection

Project No.

5,2 A

5405194 / 5601143 / 5773287 / 6079403

Contains fluorinated greenhouse

KB (E3.1 + E5.1) 11/2014 page 10/100

Tel. + 49 (0) 7462/ 2005-0

E5.1 Made in Germany

Figure 2: Type plate (example of KB 240 regular unit)

Indications of the type plate

Information

BINDER

Manufacturer: BINDER GmbH

KB 240

Model designation

Cooling incubator

Device name

Serial No.

00-00000

Serial no. of the unit

Built

2014

Year of construction

100 °C
212°F

Enclosure protection

IP 20

IP type of protection acc. to EN 60529

Temp. safety device

DIN 12880

Temperature safety device acc. to standard DIN 12880

Class

3.1

Class of temperature safety device

Art. No.

9020-0241

Art. no. of the unit

Project No.

---

Optional: Special application acc. to project no.

1,20 kW

Nominal power

200-240 V 1 N ~

Nominal voltage +/-10%, phase indication

5,2 A

Nominal current

50 Hz

Power frequency

Max. operating pressure 15 bar

Max. operating pressure in the refrigerating system

R 134A - 0,35 kg

Refrigerant type and max. filling weight

Contains fluorinated greenhouse
gases covered by the Kyoto Protocol

Contains fluorinated greenhouse gases covered by the Kyoto
Protocol

Symbol on the type plate

Information

LABORATORY EQUIPMENT
43KM

Nominal temperature

Nominal temperature

CE conformity marking

Electrical and electronic equipment manufactured / placed on the market
in the EC after 13 August 2005 and to be disposed of in separate
collection according to directive 2002/96/EC on waste electrical and
electronic equipment (WEEE).

(KB-UL only)

The equipment is certified in the GOST R certification system of
GOSTSTANDARD Russia.

The equipment is certified by Underwriters Laboratories Inc.® according
to standards CAN/CSA-C22.2 No. 61010-1, 2nd Edition, 2004-07
(Electrical Equipment for Measurement, Control, and Laboratory Use;
Part 1: General Requirements); UL 61010-1, 2nd Edition, 2005-07-22
(Electrical Equipment for Measurement, Control, and Laboratory Use;
Part 1: General Requirements); IEC 61010-1:2001, 2nd Edition and IEC
61010-2-10 (Particular Requirements for Laboratory Equipment for the
heating of materials).

KB (E3.1 + E5.1) 11/2014 page 11/100

1.5 General safety instructions on installing and operating the cooling incubator

With regard to operating the cooling incubator KB and to the installation location, please observe the
guideline BGI/GUV-I 850-0 on safe working in laboratories (formerly BGR/GUV-R 120 or ZH 1/119
laboratory guidelines issued by the employers’ liability insurance association) (for Germany.

BINDER GmbH is only responsible for the safety features of the unit provided skilled electricians or
qualified personnel authorized by BINDER perform all maintenance and repair, and if components relating
to chamber safety are replaced in the event of failure with original spare parts.

To operate the unit, use only original BINDER accessories or accessories from third-party suppliers
authorized by BINDER. The user is responsible for any risk caused by using unauthorized accessories.

CAUTION

Danger of overheating.

Damage to the unit.

∅ Do NOT install the unit in unventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

Do not operate the cooling incubator KB in hazardous locations.

DANGER

Explosion hazard.

Danger of death.

∅ Do NOT operate the unit in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the unit.

The cooling incubator KB does not dispose of any measures of explosion protection.

DANGER

Explosion hazard.

Danger of death.

∅ Do NOT introduce any substance into the cooling incubator which is combustible or

explosive at working temperature.

∅ NO explosive dust or air-solvent mixture in the inner chamber.

Any solvent contained in the charging material must not be explosive or inflammable. I.e., irrespective of
the solvent concentration in the steam room, NO explosive mixture with air must form. The temperature
inside the chamber must lie below the flash point or below the sublimation point of the charging material.
Familiarize yourself with the physical and chemical properties of the charging material, as well as the
contained moisture constituent and its behavior with the addition of heat energy.

Familiarize yourself with any potential health risks caused by the charging material, the contained moisture
constituent or by reaction products which may arise during the temperature process. Take adequate
measures to exclude such risks prior to putting the cooling incubator into operation.

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DANGER

Electrical hazard.

Danger of death.

∅ The unit must NOT become wet during operation or maintenance.

The cooling incubators were produced in accordance with VDE regulations and were routinely tested in
accordance to VDE 0411-1 (IEC 61010-1).

During and shortly after operation, the temperature of the inner surfaces almost equals the set-point.

CAUTION

The glass doors, the glass door handles, and the inner chamber will become hot
during operation.

Danger of burning.

∅ Do NOT touch the glass doors, the glass door handles, the inner surfaces or the

charging material during operation.

WARNING

Stability hazard.

Danger of injury.

Damage to the unit and the charging material.

Housing cover breakaway.

∅ Do NOT climb on the lower housing cover.

∅ Do NOT load the lower housing cover with heavy objects while the unit door is open.

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1.6 Intended use

Cooling incubators KB are suitable for exact conditioning of harmless materials. Because of their precise
temperature accuracy these devices are especially useful for cultivation of microorganisms with a narrow
temperature optimum in a range of 4 °C / 39.2°F to 37 °C / 98.6°F. Main fields of application are tests of
long-term storage (e.g. at 4 °C / 39.2°F), refrigerated incubation between 20 °C / 68°F and 25 °C / 77°F
and incubation at 37 °C / 98.6°F (also with additional introduction of heat) or with alternating temperatures
(e.g. 37 °C / 98.6°F and 4 °C / 39.2°F).

A mixture of any component of the charging material with air must NOT be explosive. The operating
temperature must lie below the flash point or below the sublimation point of the charging material. Any
component of the charging material must NOT be able to release toxic gases.

Other applications are not approved.

Cooling incubators KB are not classified as medical devices as defined by the Medical Device
Directive 93/42/EEC.

Following the instructions in this operating manual and conducting regular maintenance work
(chap. 17.1) are part of the intended use.

DANGER

Explosion or implosion hazard.

Danger of poisoning.

Danger of death.

∅ Do NOT introduce any substance combustible or explosive at working temperature into

the constant climate chamber.

∅ NO explosive dust or air-solvent mixture in the inner chamber.

∅ Do NOT introduce any substance which could lead to release of toxic gases.

The charging material shall not contain any corrosive ingredients that may damage the
machine components made of stainless steel, aluminum, and copper. Such ingredients
include in particular acids and halides. Any corrosive damage caused by such ingredients is
excluded from liability by BINDER GmbH.

WARNING: If customer should use a BINDER chamber running in non-supervised continuous
operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples
to split such specimen or samples and store them in at least two chambers, if this is feasible.

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2. Unit description

A high level of precision, reliability, and safety for all growth parameters ensures optimum incubation
conditions. Moreover, the KB cooling incubator is designed for maximum usability – even in continuous
operation year after year. It fulfills all technical and application-specific requirements arising in
experimentation such as in the areas of biotechnology, medicine, the nutrition industry, pharmaceutical
and cosmetics industries, botany, and zoology.

Two important temperature technologies have been combined to achieve perfect temperature control. The
specially developed DCT™ refrigerating system, a direct refrigerating process, in conjunction with the
APT.line™ preheating chamber technology, satisfies the unique prerequisites for attaining highly-precise
temperature control and particularly short recovery times after opening the door.

The refrigerating system is distinguished by direct, precise, and rapid temperature conduction. Large-area
labyrinth evaporator plates directly conduct the cold to the atmosphere of the working space.

The APT.line™ preheating chamber system ensures high level of spatial and time-based temperature
precision, thanks to the direct and distributed air circulation into the interior. This is especially important for
maintaining temperatures – especially with full chambers – and for rapid restoration of optimum growth
conditions after opening the door. The inner glass door ensures that the temperature remains constant
when observing the incubation process. The fan supports exact attainment and maintenance of the
desired temperature accuracy. The fan speed is digitally adjustable. The heating and refrigerating systems
are microprocessor regulated to a tenth of a degree. In addition, the cooling incubator provides almost
unlimited possibilities for adaptation to individual customer requirements based upon extensive
programming options and on the week program timer and real time clock of the controller.

All unit functions are easy and comfortable to use thanks to their clear arrangement. Major features are
easy cleaning of all unit parts and avoidance of undesired contamination.

The inner chamber, the pre-heating chamber and the interior side of the doors are all made of stainless
steel V2A (German material no. 1.4301, US equivalent AISI 304). The housing is RAL 7035 powder­coated. All corners and edges are also completely coated.

The cooling incubator KB comes equipped with a serial interface RS 422 for computer communication,
e.g. via the communication software APT-COM™ 3 DataControlSystem (option, chap. 16.1). For further
options, see chap. 20.5.

The models KB 240, KB 400, and KB 720 are equipped with
locked via the attached brakes.

Temperature range at ambient temperature of 25 °C / 77 °F: -5 °C / 23 °F up to +100 °C / 212 °F

four castors. Both front castors can be easily

KB (E3.1 + E5.1) 11/2014 page 15/100

(3)

(2)

(1)
(4)

(5)

(2)

(1)
(4)

(3)
(5)

B NE

I

2.1 Unit overview

Figure 3: Cooling incubator KB (examples)

(1) Main power switch on/off
(2) Temperature controller RD3
(3) Instrument box
(4) Unit door
(5) Refrigerating module

KB (E3.1 + E5.1) 11/2014 page 16/100

2.2 Control panel

(2) (6) (1)

Figure 4: Control panel

(1) Main power switch on/off
(2) Program controller RD3

(12) (2) (6) (11) (1)

Figure 5: Control panel KB 23 / 53 / 115 (E3.1)

with option temperature safety device class 3.3 and interior illumination

(1) Main power switch on/off
(2) Program controller RD3
(6) Switch for interior illumination (option)
(11) Temperature safety device class 3.1 (part of option safety device class 3.3)
(12) Temperature safety device class 3.2 (part of option safety device class 3.3)

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2.3 KB (E5.1) 240 / 400 / 720: Lateral control panel at the right side (option)

(8)

(7)

(9)

(10)

Figure 6: Lateral control panel at the right side of the refrigerating machine

with options analog output, zero-voltage relay outputs via operation lines,

Ethernet interface, and additional Pt 100 temperature sensor

(7) DIN socket for analog output 4-20 mA (option)
(8) DIN-socket for zero-voltage relay outputs via operation lines (option)
(9) DIN-socket for additional Pt 100 temperature sensor (option
(10) Ethernet interface and MAC address for computer communication (option)

KB (E3.1 + E5.1) 11/2014 page 18/100

2.4 KB (E5.1) 240 / 400 / 720: Lateral control panel at the left side (option)

(11)

(12)

(11) Temperature safety device class 3.1 (part of option safety device class 3.3)
(12) Temperature safety device class 3.2 (part of option safety device class 3.3)

Figure 7: Lateral control panel (option) at the left side of the refrigerating machine

with option temperature safety device class 3.3

KB (E3.1 + E5.1) 11/2014 page 19/100

2.5 Unit rear

(13)

(14)

Figure 8: Unit rear with position of RS 422 interface

(13) Interface RS 422 for computer communication
(14) Power cable

3. Completeness of delivery, transportation, storage, and installation

3.1 Unpacking, and checking equipment and completeness of delivery

After unpacking, please check the unit and its optional accessories, if any, based on the delivery receipt
for completeness and for transportation damage. Inform the carrier immediately if transportation damage
has occurred.

The final tests of the manufacturer may have caused traces of the shelves on the inner surfaces. This has
no impact on the function and performance of the unit.

Please remove any transportation protection devices and adhesives in/on the unit and on the doors and
remove the operating manuals and accessory equipment.

CAUTION

Sliding or tilting of the unit.

Damage to the unit.

Risk of injury by lifting heavy loads.

∅ Do NOT lift or transport the unit using the door, the handle, or the lower housing.

∅ Do NOT lift units size 720 by hand.

 Lift units size 23. 53 und 115 from the pallet with the aid of four people in the area of all

KB (E3.1 + E5.1) 11/2014 page 20/100

4 unit feet.

 Lift units size 240 from the pallet with the aid of six people in the area of all 4 unit feet

or use a fork lifter. Set the fork lifter only from the front or rear in the middle of the unit.

 Lift units size 720 using technical devices (fork lifter) from the pallet. Set the fork lifter

only from the front or rear in the middle of the unit.

∅ Do NOT set the fork lifter from the unit side.

Set the fork lifter only

If you need to return the unit, please use the original packing and observe the guidelines for safe lifting
and transportation (chap. 3.2).

For disposal of the transport packing, see chap. 18.1.
Note on second-hand units (Ex-Demo-Units):
Second-hand units are units that were used for a short time for tests or exhibitions. They are thoroughly

tested before resale. BINDER ensures that the chamber is technically sound and will work flawlessly.
Second-hand units are marked with a sticker on the unit door. Please remove the sticker before

commissioning the unit.

3.2 Guidelines for safe lifting and transportation

The front castors of units size 240, 400, and 720 can be blocked by brakes. Please move the units with
castors only when empty and on an even surface, otherwise the castors may be damaged. After
operation, please observe the guidelines for temporarily decommissioning the unit (chap. 18.2).

CAUTION

Sliding or tilting of the unit.

Damage to the unit.

Risk of injury by lifting heavy loads.

∅ Transport the unit in its original packaging only.

∅ For moving or shipping, secure the unit with transport straps.

∅ Do NOT lift or transport the unit using the door, the handle, or the lower housing.

∅ Do NOT lift units size 720 by hand.

 Lift units size 23. 53 und 115 with the aid of four people in the area of all 4 unit feet.
 Lift units size 240 with the aid of 6 people or with a fork lifter.

from the front or rear in the middle of the unit.

 Lift units size 720 using technical devices (fork lifter). Set the fork lifter only from the

front or rear in the middle of the unit.

∅ Do NOT set the fork lifter from the unit side.

• Permissible ambient temperature range during transport: -10 °C / 14°F to +60 °C / 140°F.

You can order transport packing for moving or shipping purposes from BINDER service.

3.3 Storage

Intermediate storage of the unit is possible in a closed and dry room. Observe the guidelines for
temporary decommissioning (chap. 18.2).

• Permissible ambient temperature range during storage: -10 °C / 14°F to +60 °C / 140°F.

• Permissible ambient humidity: max. 70 % r.H., non-condensing

When after storage in a cold location you transfer the unit to its warmer installation site, condensation may
form. Before start-up, wait at least one hour until the chamber has attained ambient temperature and is
completely dry.

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3.4 Location of installation and ambient conditions

Set up the cooling incubator KB on a flat, even surface, free from vibration, in a well-ventilated, dry
location and align it using a spirit level. The site of installation must be capable of supporting the unit’s
weight (see technical data, chap. 20.4). The chambers are designed for setting up inside a building
(indoor use).

CAUTION

Danger of overheating.

Damage to the unit.

∅ Do NOT set up units in non-ventilated recesses.

 Ensure sufficient ventilation for dispersal of the heat.

• Permissible ambient temperature range during operation: +18 °C / 64.4°F to +32 °C / 89.6°F. At

elevated ambient temperature values, fluctuations in temperature can occur.

The ambient temperature should not be substantially higher than the indicated ambient
temperature of +25 °C / 77°F to which the specified technical data relates. Deviations from the
indicated data are possible for other ambient conditions.

With each degree of ambient temperature > +25 °C / 77°F, the refrigeration power decreases
by 1.5 K.

• Permissible ambient humidity: 70 % r.H. max., non-condensing.

When operating the chamber at temperature set-points below ambient temperature, high ambient
humidity may lead to condensation on the unit.

• Installation height: max. 2000 m / 6.6 ft. above sea level.

When placing several units of the same size side by side, maintain a minimum distance of 250 mm / 9.84
in between each unit. Wall distances: rear 100 mm / 3.94 in, sides 160 mm / 6.29 in. Spacing above the
unit of at least 100 mm / 3.94 in must also be maintained.

Two KB (E3.1) units size 23, 53 or 115 of the same size can be piled on top of each other. For this
purpose place rubber pads under every foot of the upper unit to prevent the device from slipping.

CAUTION

Sliding or tilting of the upper unit.

Damage to the units.

 When stacking, place rubber pads under every foot of the upper unit.
 Stack only units of the same size..

KB (E5.1) units size 240, 400 und 720 must NOT be stacked.

CAUTION

Danger by stacking.

Damage to the units.

∅ Do NOT place the cooling incubators on top of each other.

To completely separate the unit from the power supply, you must disconnect the power plug. Install the
unit in a way that the power plug is easily accessible and can be easily pulled in case of danger.

For the user there is no risk of temporary overvoltages in the sense of EN 61010-1:2010.

KB (E3.1 + E5.1) 11/2014 page 22/100

With an increased amount of dust in the ambient air, clean the condenser fan (by suction or blowing)
several times a year.

Avoid any conductive dust in the ambiance according to the unit layout complying with pollution degree 2
(IEC 61010-1).

Do not install or operate the unit in potentially explosive areas.

DANGER

Explosion hazard.

Danger of death.

∅ Do NOT operate the unit in potentially explosive areas.

 KEEP explosive dust or air-solvent mixtures AWAY from the vicinity of the unit.

4. Installation of the equipment

4.1 Spacers for wall distance (KB 240, 400, 720)

Please fix both spacers with the supplied screws at the unit rear. This serves to ensure the prescribed
minimum distance to the wall of 100 mm / 3.94 in.

Figure 9: Spacer for rear wall distance

Figure 10: Rear KB (E5.1) with mounted spacers

KB (E3.1 + E5.1) 11/2014 page 23/100

4.2 Electrical connection

The cooling incubators KB are supplied ready for connection.

• The cooling incubator KB (E3.1) comes with a fixed power connection cable that has a length of 1800

mm / 5.9 ft and is equipped with a semi time-lag fine-wire fuse.

• The cooling incubator KB (E5.1) comes with a fixed power connection cable that has a length of 1800

mm / 5.9 ft.

Model Power plug

KB 23 (E3.1)

KB 53 (E3.1)

KB 115 (E3.1)

KB 23-UL (E3.1)

KB 53-UL (E3.1)

KB 115-UL (E3.1)

KB 240 (E5.1)

KB 400 (E5.1)

KB 720 (E5.1)

KB 240-UL (E5.1)

KB 400-UL (E5.1)

KB 720-UL (E5.1)

• The socket must also provide a protective conductor.

• Prior to connection and start-up, check the power supply voltage. Compare the values to the specified

data located on the unit’s type plate (left unit side, bottom right-hand, see chap. 1.4).

Shock-proof plug 230 V 1N~ 50/60 Hz 10 Amp

Shock-proof plug 230 V 1N~ 50 Hz 10 Amp

NEMA 5-15 115 V 1N~ 60 Hz 12,5 Amp

Shock-proof plug 200 V to 240 V 1N~ 50 Hz 16 Amp

Shock-proof plug 200 V to 240 V 1N~ 50/60 Hz 16 Amp

NEMA 5-20P 100 V to 120 V 1N~ 60 Hz 16 Amp

NEMA 5-20P 100 V to 120 V 1N~ 50/60 Hz 16 Amp

NEMA 6-20P 200 V to 240 V 2 ~ 50/60 Hz 16 Amp

Voltage
+ /-10 %

Current

type

Power

frequency

Unit fuse

• When connecting, please observe the regulations specified by the local electricity supply company as

well as the VDE directives (for Germany). We recommend the use of a residual current circuit breaker.

• Pollution degree (acc. to IEC 61010-1): 2

• Over-voltage category (acc. to IEC 61010-1): II

CAUTION

Danger of incorrect power supply voltage.

Damage to the equipment.

 Check the power supply voltage before connection and start-up.
 Compare the power supply voltage with the data indicated on the type plate.

See also electrical data (chap. 20.4).

To completely separate the unit from the power supply, you must disconnect the power plug.
Install the unit in a way that the power plug is easily accessible and can be easily pulled in case
of danger.

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(3a) LED Heating active

lluminates: program

Display 1

Display 2

5. Start up

After connecting the electrical supply (chap. 4.1), turn on the unit by the main power switch (1).
Warming chambers may release odors in the first few days after commissioning. This is not a quality

defect. To reduce odors quickly we recommend heating up the chamber to its nominal temperature for
one day and in a well-ventilated location.

WARNING: If customer should use a BINDER chamber running in non-supervised continuous
operation, we strongly recommend in case of inclusion of irrecoverable specimen or samples
to split such specimen or samples and store them in at least two chambers, if this is feasible.

5.1 Settings at the RD3 program controller

After turning the unit on with the main power switch (1) the controller is in Normal Display / Fixed value
operation mode.

Depending on the temperature value entered before, LED (3a) is lit if the heating is active, or LED (3b) if
the refrigeration is active, or no LED if the actual temperature is equal to the set-point.

In Display 1 of the controller the actual temperature value is shown.

• With inactive week program timer:

In Display 2 of the controller the actual date and time are displayed. Example:

• With active week program timer:

In Display 2 of the controller the actual date and time and the states of the week program timer
channels are displayed. Examples:

15.05.14 13:52 - - 15.05.14 13:52 - �

Channel 1 Off,
Channel 2: Off

15.05.14 13:52

Channel 1: Off,

Channel 2: On

15.05.14 13:52 � -

Channel 1: On,

Channel 2: Off

15.05.14 13:52 � �

Channel 1 On,

Channel 2: On

(3b) LED Refrigeration active

(no function)

(3d)
LED i

operation
LED flashes: exceeding of the

tolerance limits in Fixed value
entry mode or in Program
operation. In program operation:
program interruption.

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Figure 11: RD3 program controller

The program controller RD3 permits programming of temperature cycles. For each program section also
the fan speed can be regulated.

Two programs with up to 10 sections each or one program with up to 20 sections can be entered (setting
in the user level, chap. 10).

When changing from 2 programs to 1 program or vice-versa, existing programs are deleted

The maximum length of an individual program section can be set to either 99 hs 59 min or to 999 hs 59
min (setting in the user level, chap. 10). This setting is then valid for all program sections.

Programming can be done directly via the controller keyboard or graphically at the computer using the
communication software APT-COM™ 3 DataControlSystem (option, chap. 16.1) specially developed by
BINDER.

5.2 General indications

The program controller RD3 offers several functional levels:

Normal Display / fixed value operation:

• Display of the actual value of temperature (display 1) and of the actual date and time (display 2).

• The chamber is in fixed value operating mode, equilibrating to the entered set-points.

Fixed value entry mode (chap. 6)

• Entry of set-points for temperature, fan speed, and the safety controller for fixed value operating mode

• Entry of temperature set-points SP 1 and SP 2 for week program operation

Program editor (chap. 8)

• Two programs with up to 10 sections each or one program with up to 20 sections can be entered

(selection in the user level, chap. 10). Entry of set-points for temperature and fan speed in all program
sections (chap. 8.2).

• Deleting a program section (chap. 8.4)

Program start level (chap. 9)

• Selection of an entered program

• Entry of settings affecting the program course, as “start delay time” or “number of program cycles”

• Program start

Week program editor (chap.7)

• Setting the shift points

User level (chap. 10)

• User specific controller settings

• Setting the real time clock

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Menu visible only if week program

timer is activated in the user level

Display 1 shows

e.g. 19.8

(actual temperature value)

in the user level, chap. 10)

Display 1 shows

e.g. 10.0

(actual temperature set-point 1)

Display 2 shows

SP1 TEMPERATURE

(variable: temperature in °C)

Normal Display / Fixed value operation

5 seconds

Fixed value entry mode

If no button is touched within more than 120 sec. the controller returns from the current level to Normal
Display.

Program editor

5 seconds

Week program editor

5 seconds

User level

Program start level

6. Fixed value entry mode

If you do not want to use the week program timer, deactivate it (factory setting, setting in the
user level, chap. 10) before entering any set-points. Any setting of the operation lines in Fixed
value entry mode is ineffective with an active week program timer.

Basic entry principle: Access the individual parameters with button X/W one after the other. Enter the
values with the arrow keys. A value flashing once after 2 seconds indicates that it has been applied by the
controller.

Normal Display

(actual date and time)

Display 2 shows e.g. 15.05.06 13:52 - -

Press key

Enter temperature set-point in °C using

arrow keys

(actual switching state of week program timer channel 1: Off,
channel 2: Off; visible only if week program timer is activated

Value is shown in display 1.

KB (E3.1 + E5.1) 11/2014 page 27/100

in the user level, chap. 10)

Display 2 shows

SP2 TEMPERATURE

(variable: temperature in °C)

Display 1 shows

e.g. 100

(actual fan speed set-point)

Display 2 shows

SP FAN SPEED

(variable: fan speed in %)

Display 1 shows

e.g. 000

(actual switching state of operation lines)

Display 2 shows

OPERATION LINE

(variable: switching state of operation lines)

Display 1 shows

e.g. 40

(actually set safety controller set-point)

Display 2 shows

SP SAFETY CONTR.

(variable: safety controller set-point)

Press key

Display 1 shows e.g. 37.0

Enter temperature set-point in °C using

arrow keys

Press key

Enter fan speed set-point in % using arrow

Press key

Only with option zero-voltage relay outputs via operation lines (chap. 16.4):

Enter switching state using arrow keys

(actual temperature set-point 2)
(visible only if week program timer is activated

Value is shown in display 1.

Value is shown in display 1.

keys

Set safety controller class 3.1 set-point in °C

using arrow keys

Note setting “limit” or “offset” selected at the
user level (chap. 10)!
Value is shown in display 1.

Press key

If no button is pressed within more than 120 sec, or if the EXIT button is pressed, the controller changes
to Normal Display.

When changing the set-point, check the safety controller setting in the user level (chap. 10) if
the safety controller has been set to “limit” mode.

If the fan is operated with less than 100 % speed, the temperature performance and the spatial
exactitude of the temperature can differ from the manufacturer’s specifications. Do reduce the
fan speed only if absolutely necessary due to special requirements.

KB 23: Cooling power decreases when the fan speed is reduced to 20% or below.

The values entered in fixed-value entry mode remain valid after program run-off and are then
equilibrated.

If the week program timer is active, depending on the running week program another set-point (SP2) may
be targeted. Too high or too low temperatures can occur which exceed the permissible temperature of the
charging material. Deactivate the week program timer if you do not use it (default setting, setting in the
User level, chap. 10).

KB (E3.1 + E5.1) 11/2014 page 28/100

CAUTION

Display 1 shows

e.g. 19.8

(actual temperature value)

(actual date and time, actual state of week program timer

channel 1: Off, channel 2: Off)

Display 1 shows

e.g. 0000

Display 2 shows

PROGRAM EDITOR

(you are in the program editor)

Menu visible only if week program timer is activated

in the user level (chap. 10)

Display 2 shows

WEEK PROG. EDITOR

(you are in the week program editor)

Display 1 shows

0000

Display 2 shows

UserCod?

0000

(enter user code, display flashes)

Display 1 shows

0000

(selection of day of the week)

(actual selection: Monday)

Display 1 shows

0000

Display 2 shows

Shiftpt.

(no function)

Too high or too low temperature after the program ends.

Damage to the charging material.

 Deactivate the week program timer if you do not use it.

7. Week program editor

The Week program editor permits defining up to 4 shift point for each week day. A shift point defines a
moment and the switching state ON or OFF of the channels that become active in this instance.

Channel function:

• Channel 1 On = Set-point 2 is equilibrated.

• Channel 1 Off = Set-point 1 is equilibrated

• Channel 2 = reserve

The week program timer is initially set to inactive (factory setting). Therefore, you need to
activate the week program timer in the user level (chap. 10).

Normal Display

Display 2 shows e.g. 15.05.06 13:52 - -

Press down key

Press down key

Display 1 shows 0000

Press program key

Enter the user code using arrow keys

Automatically forward after 2 sec

Display 2 shows Monday

for 5 sec

for 5 sec

e.g. 0001 (basic setting, adjustable in the
user level, chap. 10).
Value is shown in both displays.

Select the day of the week (Monday up to

Sunday) with key

Press program key

Press program key

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Day of the week is shown in display 2.

Display 1 shows

0000

(selection of the shift point)

(actual shift point: 1)

Display 1 shows

e.g. --.--

(time of the selected shift point)

(actual selection of the shift point: S1)

(actual setting: shift point not programmed)

Display 1 shows

--.--

(time of the selected shift point)

(entry of the time of the selected shift point)

(actual setting: shift point not programmed)

Display 1 shows

0000

(entry of the state of channel 1)

(actual setting: Off)

Display 1 shows

0000

(entry of the state of channel 2) (no function)

(actual setting: Off)

Display 1 shows

e.g., 08.30

(time of the selected shift point)

(actual selection of the shift point: S1)

(actual setting: time 08.30, channels Off)

Press key EXIT

Press program key

Display 2 shows Shiftpt. 1

Select the shift point (1 up to 4) with key

Press program key

Display 2 shows S1: --:--

Press program key

Display 2 shows Time --:--

Enter the time (hh:mm) using arrow keys

Press key

Display 2 shows Ch1 = SP2: Off

Enter the state of channel 1

(On or Off) using arrow keys

Value is shown in display 2.

Value is shown in display 2.

Setting is shown in display 2.

Press key

Display 2 shows Channel 2: Off

Enter the state of channel 2

(On or Off) using arrow keys

Press key

Display 2 shows S1: 08:30 - -

Select the next shift point Select the next day of the week

Setting is shown in display 2.

Press key EXIT

Press key EXIT twice

To exit the menu, press the EXIT key several times or wait for 120 seconds. The controller returns to
normal display.

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