OPERATING MANUAL
1
TION
Table of Contents
NO. CONTENTS PAGE
1 SAFETY AND GENERAL PRECAU
2 FEATURES AND SPECIFICATIONS 5
3 CONTROL PANEL 6
4 INSTALLATION 7
5 CO2 INCUBATOR OPERATION 8-11
6 ORBITAL SHAKER OPERATION 11-13
3-4
2
1. General Information and Precautions
Safety Symbols:
1.1. Precautions related to the power cable
Always al low at least 3cm between the power cable and the back wall to prevent the back wall from causing
pressure on the power cable.
Always use the electrical plug that was supplied with this instrument.
Never touch the power cord with wet hands. (This can result in electric shock.)
Never use a damaged power cord or power outlet.
In the event of smoke or a burning smell, immediately remove the power cord from the outlet.
1.3. Precaution for use
Do not attempt to disassemble this instrument. If service is required, please contact your local
representative.
Never operate a flammable spray near this instrument. (This can result in a fire hazard.)
Always use caution when using flammable substances such as benzene, alcohol
and LP gas. (Failure to do so can result in a fire hazard.)
Prevent foreign substance s from contacting the door seal. (The inflow of outside air can negatively
impact the temperature in chamber.)
Permissible ambient temperature range for transport: -10℃ to 60℃.
3
ALWAYS ensure that the instrument is connected to an outlet with specifciations that match those of the serial number label.
(Over-v oltage or under-voltage can damage the product and result in poor performance.)
When installing the instrument, always allow a di stance of at least 30cm from the back wall.
Install the unit in the way that the power plug is easily accessible
and can be easily pulled in case of danger.
Install the instrument on a flat and stable s urface, free from vibration and in a well-ventilated location. (If the surface is not level, it can cause an excessive vibration of the product.)
Excessive CO2 can be harmful when in high concentrations.
Any excess amount of CO2 should be properly ventilated or by connection to a suitable
exhaust system.
4
2. FEATURES AND SPECIFICATIONS:
2.1 Features
1. Excellent temp erature uniformity of Temperature and CO
with a built in shaker
2
2. 6 Side direct heat for temperature uniformity and fast recovery
3. IR CO₂ Sensor detects precise density of CO₂
4. Outer heated door prevents condensation on glass viewing door.
5. Microprocessor PID control for Temperature, CO₂ and shaking speed
6. Natural Humidification System by water tray and circulation fan.
7. Simple, user-friendly operation for shaker apparatus.
2.2 Specification
Items NB203QMS
Temperature
Range
Accuracy ±0.5℃ at 37℃
Controller Microprocessor On Off Control
Humidity 70% ~ 80% at 37℃
Ambient +5℃ to 60℃ *
CO2
Range 0% to 20%
Accuracy ±0.1% at 5% at 37℃ setting
CO2 Increment 0.1%
CO2 Sensor IR CO2 Sensor
Shaker
RPM 30 to 300 rpm
RPM Accuracy ±1%
Increment 1 RPM
Orbital throw 19mm
Motor BLDC (Plate Type Brushless DC Motor)
TIME Range Continuous or up to 47hrs 59min
Platform 250(W) x 310(D)mm with Silicon Rubber Pad
Outer door Silicon Magnet Packing Door
Inner door Tempered Glass Door
Jacket type Dry wall type (6 sides direct heating type)
Chamber material STAINLESS STEEL
Weight
Capacity / Shelve 47Liter / 1 shelf
Chamber dimension 335(W) x 361(D) x 392(H)mm
Overall dimension 420(W) x 465(D) x 553(H)mm
Power 110/220V, 50/60Hz (AC 230V : 350W , AC 115V : 340W)
*The minimum temperature specification may vary depending on the room temperature and shaking speed.
5
3.Control Panel
1 2 3 4 5 6
7 8 9 10 11 12 13 14 15 16 17
1. SAFETY activation indicator: If the over-temperature safety is activated, this LED will be ON
2. Temperature display LED window
3. CO₂ Gas % display window
4. CO
5. RPM display : RPM value display
6. Shaker Status Pilot Lamp : When Shaker is running, this lamp is flashing.
7. Alarm On/Off Switch
8. Alarm Mute Button : When pressed, provides a10 minutes audible alarm delay.
9. Heating Signal: Shows the status of each heating at the 3 sensor locations. (Glass door = heater around
10. Temperature Setting Button.
11. Adjustment button
12. CO₂ Gas Setting Button
13. Calibration Mode button
14. Display Mode Selection Button either RPM or TIME
15. RPM setting Button
16. Adjustment button
17. Shaker Start/Stop Button
Supply Status Pilot Lamp : Pilot lamp ON means inflow of gas
2
glass door.)
6
4. Installation
Before beginning the installation, always:
Inspect the packaging for damage. When the instrument is received, please inspect the item carefully to check for any
potential transit damage. In the event of dam age, always report the damage to t he shipping carrier and your local
representative imm ediately.
Included in the package:
1. Stainless Steel Perforated shelf – 1 Pcs
2. Power Cord – 1 Pcs
3. Stainless Steel Water Container for humidity- 1Pc
4. CO₂ Gas Tubing with in-line CO₂ Gas filter – 1Pcs
5. Operating Manual- 1Pcs Above parts are packed inside incubator . When received, open the door of incubator and remove all parts to check
confirm receipt.
Cleaning before use
Before conductin g cell culture in this m ini incubator, It is recom mended to clean up entire cham ber and shelves and
water container by using soft cloth with at least 70% Ethanol mixed of 30% distilled water.
Installation Procedure:
4.1 Place the incubator at the desired location.
Always avoid placing the incubator in:
- An area near equipment generating heat or cold air
- Direct sunlight
- An uneven surface or table.
- A place with heavy vibration
- A place with little air ventilation space behind the incubator.
4.2 Place the shelves and water container as shown in chapter “CONFIGURATION”. If desired, place the stainless
steel water container at bottom of chamber toward the back of incubator (in order to get it to be close to circulation fan).
This increases the humidity.
Distilled water is recommended in order to avoid contamination and corrosion.
If possible, use warm (~37℃) distilled water for immediate humidification.
4.3. Connect the CO₂ Gas supply
The Gas tub es pro vide d is a 6mm (dia) tu be. Ins ert th e e dge of tube to gas in let port and conn ect it to a gas regu lator
which should be installed on the gas cylinder. Or, install to the gas line in your lab.
-Turn on the gas supply with the pressure set to 1 Bar (or 14.5 Ps i)
NOTE! :
water to each fitting and check if an y bubbles are gene r ated. If so, readjust the fitting.
4. Connect the power cord.
-Plug the power cord onto the power inlet on the back side of the incubator (refer to CONFIGURATION)
-Plug the power plug into an outlet of the appropriate voltage.
To confirm that there are no le aks in the CO₂ connections, a “bubble check” is recommended. Ap ply soapy
7
5. CO₂ Incubator Operation
5.1 Power switch
Turn on the power switch. The digital LED will display current temperature and CO₂% in the chamber.
5.2 Setting temperature
a. Press the "TEMP SET" key, then, the LED screen will flash and displa y current pr ogrammed temperature.
b. Set up the desired temperature by pressing UP (▲) or DOWN (▼).
c. Press "TEMP/SET" key after adjustment. The “SAVE” message is shown on the display.
Note: Always press the “SET/TEMP” key after adjusting to the desired value. Otherwise, the value will not be saved.
5.3 Setting CO2
a. Press "CO2 SET" key. Then, the LED screen will begin flashing.
b. Input the desired value of Co2 density by adjusting UP (▲) or DOWN (▼) key
c. Press "SET" key again after inputting the value. “SAVE” is shown up on LED scr een as below.
Note: Always press the “SET/CO2” key after adjusting to the desired value. Otherwise, the value will not be saved.
5.4 Calibration for temperature and CO2
In the event of a discrepancy, please follow the below procedure for adjusting the calibration.
Measure CO2 density and Temperature after incubator is stabilized. (Allow 2 hours for stabilization after the
desired setting has been reached.
Please note that a very low deviation range such as ±0.1 ~ 0.3% could be difficult to correct precisely by this
calibration.
No. DISPLAY FUNCTION
1
Chamber Temperature
2
3
4
5
Door Heater Temperature
Glass Door Heater Temperature
CO2 Calibration
Heating control
8
6
CO2 gas supply control
7
To apply a new value
a. Press and hold “CAL/SET” for 10 seconds. Then, LED will be flickering as below.
Channel 1 is for adjusting the calibration of the main chamber temperature.
Press UP (▲) by the difference measured from the set value and the value measured by precision thermometer or
thermocouple. OR
Press DOWN (▼) by the difference measured from the set value and the value measured by precision thermometer
or thermocouple
Ex.) If measured temperature is 38℃ and Display shows 37℃ , then press up 1℃ .
Note
* Temperature Calibration range is ±5℃
th
* To proceed to next calibration channel, press “CAL/SET” button. After 5
channel, the LED is back to
temperature display.
b. Second Calibration Setting: Outer Door Temperature
The purpose of this heater is to prevent water condensation from forming on the glass door, caused by a large
temperature difference between chamber and outside air. If water is condensing on the front door, we
recommend adjusting this channel.
Note: Other than water condensing on the glass door, the calibration of channel 2 and 3 would not be recommended.
c. Third Calibration Setting: Door Frame
The purpose of this heater is to prevent water condensation from forming on the glass door, caused by a large
temperature difference between chamber and outside air. This setting should be adjusted if making a chage
to setting 2 has not resulted in preventing this condensation.
d. Fourth Calibration Setting: CO2 density calibration
9
Channel 4 is for adjusting the CO2 densit y.
Press UP (▲) by the difference measured from the set value and the value measured by precision analyzer.
Press DOWN (▼)by the difference measured from the set value and the value measured by precision analyzer.
Ex.) If the measured CO2 value is 5% and Display shows 4%, then press up to set to 1%.
e. Fifth Calibration Setting: Heating control
NOTE
Channel 5 is to set heating control point and is designed to pr event a significant temperature o vershoot. This
mode is preprogrammed in the factory and should NOT be adjusted by the user.
f. Sixth Calibration Setting: CO2 gas supply control.
NOTE
Channel 6 is to set the values assoc iated with the CO2 rele ase solenoid. This m ode is preprogrammed in the
factory and should NOT be adjusted by the user.
5.5 Alarm
10
Three m inutes after the temperature and the CO2 de nsity have reac hed the des ired setting, all alarm systems are
activated. (If the temperature or CO2 does not reach the set point, the alarm system will not be activated.) If the user
would like to disable these alarms, the alarm can be set to the “OFF” position.
Alarm Activation
Temperature: ±1℃ from set point
CO2: ± 1% from set point
Door Open: 1 minute after door opening.
*When pressing the alarm mute button on the control panel will stop the alarm from activating.
If the alarm was stopped by mute button, the alarm will be re-activated after 3 minutes.
5.6 Temperature Thermostat
In the event of a m alfunction, this thermostat k nob is designed to prevent the heat er
from overheating.
■ Always set the Safety knob to a HIGHER value than the set temperature.
■ The Safety knob has a fairly wide deviation
■ Safety knob is the safety device designed to prevent the heater from
overheating when the temperature control is malfunctioning.
This is the safety device for preventing temperature from rising caused by any circumstances.
So set the temperature at 5℃ higher than operating temperature or desired point.
■ If the “SAFET Y” LED on the front panel is illum inated, this switch has been act ivated and is limiting the
temperature.
6. Shaker Operation
6.1 T o set the desired time, Press and hold the "RPM/TIME" key for approximately 1 secon d, the LED displays "t”
followed by the current set time. Press the "SET" key locate d underneath the time/speed displa y. The set time is
displayed and will blink continuously. It can be adjusted by using the UP (▲) and DOWN (▼) keys. Once the desired
time is displayed, press the “SET” key to confirm.
* If you do not press th e "SET" key to store the d esired time, the new tim e will not be saved an d the set time will
return to the prior setting.
6.2 To set the desired mixing sp eed, Press and hol d the "RPM/TIME" key for ap proximately 1 secon d until LED
displays “r” followed by the current set mixing speed. Press the "SET" key located underneath the time/speed display.
The set speed is displayed and will blink continuously. It can be adjusted by using the UP (▲) and DOWN (▼) keys.
Once the desired speed is displayed, press the “SET” key to confirm.
* If you do not press the "SET" key to store the desired speed the new speed will not be saved and the set speed will
return to the prior setting.
11
6.3 Platform Installation
The Orbi-Shaker includes a f lat non-slip platform for lo w speed operat ion with m any comm on vessels, i ncluding tra ys
flasks and dishes. For installation of an op tional platf orm, such as the optional MAGic Clam p platform, please follow
the instructions below:
1.) Unscrew the four m etal thum b screws from the cor ners of the intern al (included) platform and remove the
green rubber mat.
2.) Place the MAGic Clamp platform (with mat) on top of the included platform and retighten the four (previously
removed) thumb screws.
6.4 Shaker Error Message
1) This message “rErrL” represents a low speed speed error
- Check the platform to ensure that nothing is obstructing it’s movement
- Check the set speed to make sure that it is within the acceptable range (30 to 300rpm)
Solution : Use your hand t o physically move platf orm. W hile the platform is still moving press and ho ld the "STOP"
and "SET" keys sim ultaneously for two seco nds. The s peed display will blink and can now be adj usted to a higher
speed.
2) This message “rErrH” represents a high speed error.
- Remove the loaded vessels.
Solution : If a vessel has been removed, replace the vessel or restart the machine.
3) This message, “Error” represents an electrical error.
- Machine has lost power during the run.
12
Solution : Check that the p o wer c ord is properly ins tal l ed a nd r estar t or chec k that the circuit has not been int err upted
and restart.
4) Display will not illuminate
(1) Electrical connection - Please disconnect main power cord and connect again.
(2) Voltage supply - Please check to confirm the correct voltage.
(3) Check the fuse
(4) Verify voltage on the serial number label of the machine
(5) Defective power switch (check voltage at switch)
6.5 Disconnecting inside Shaker
Connector of the orbital shaker is located at upper right back wall of chamber (see below). Turn the connector counterclockwise to disconnect the shaker. Before disconnecting, ensure that incubator is powered off.
Service and Contact
Service on the instrument should only be performed by qualified service personnel.
To request service or technical support, please contact Benchmark Scientific or your
local Benchmark Scientific representative.
13
Loading...