Benchmark Scientific Benchmark Scientific MR9610 Manual

Operation Manual

Version 2.0

MR9610,

SmartReader™ UV-Vis

Microplate Reader

This instrument is for research use only

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III

Foreword

Thank you for purchasing our MR9610, SmartReader™ UV-Vis Microplate
Reader. This user manual describes how the instrument works and includes an
operation guide, please read it carefully before operation and keep for future
reference.

Initial check

Please check the instruments as well as all accessories with the packing list when
first received and unpackaged. If you find any missing, please contact your
distributor or Accuris Instruments.

Safety Warning and Guidelines

1. Important information for safe use

Please read the manual carefully and operate safely according to all

guidelines.

This instrument is intended for Research Use Only

Users should have a clear understanding on use this instrument before
operation. Please read this user manual carefully.

IV

2. Safety Tips

The operation, maintenance and repair of the Instrument should comply with the
basic guidelines and the warnings below.

Indoor use only.

Warning: Biological contamination hazard. All samples for testing,
quality control, and calibration should be regarded as infectious, and
any parts that come into contact with samples should also be treated
as infectious. Please wear gloves and appropriate safety gear when
operating this device.

Before using the device, read the operation manual carefully. These
instruments are designed for use in a laboratory environment. The
device must be operated by skilled laboratory personnel with the
appropriate training.

Warning: Avoid injury. Keep your body or any part of body at a safe
distance (1 foot or more) from the instrument when running.

The operator should not open or perform any repairs on the
Instrument without specific instructions from Accuris Instruments or
an authorized agent of Accuris. Performing any unauthorized
service repairs will result in loss of warranty coverage. Contact
Accuris Instruments or an authorized distributor or agent in the event
of any service issues.

Before powering on, confirm that the voltage used meets the
electrical requirements of the instrument as stated on the rating plate.
If the electric cord is damaged, replace it with the same type.

Hold the socket firmly when you pull the plug from an outlet, and don’t

pull the electric line only.

V

The Instrument should be installed in an environment of standard
room temperature, low dust, low humidity and away from direct
sunlight. Also make sure there is adequate ventilation, no corrosive
gas or strong magnetic field.

Always power off the instrument when you are finished using it.
Unplug the power cord and cover the instrument with a cloth or plastic
sheet to prevent excessing dust from entering the housing.

Pull the connector plug from the electrical outlet immediately and
contact the vendor in the case of:

⚫ Liquid entering the housing.
⚫ Abnormal operation: such as any abnormal sound or smell.
⚫ The instrument is dropped or there is any damage to the housing.

⚫ Any malfunction

Contents

Foreword ................................................................................................................................................ III

Initial check ........................................................................................................................................... III

Safety Warning and Guidelines .............................................................................................................. III

1. Important information for safe use .................................................................................... III

2. Safety Tips ............................................................................................................................ IV

Chapter 1 Introduction .................................................................................................................... 1

Chapter 2 Features ................................................................................................................................ 2

Chapter 3 Instrument Overview .................................................................................................... 4

Chapter 4 Installation ............................................................................................................................ 5

1. Initial check ................................................................................................................................ 5

2. Installation location and environment..................................................................................... 5

3. Installation steps ....................................................................................................................... 5

Chapter 5 Operation Guide ...................................................................................................................... 7

1. Initial self-check ......................................................................................................................... 7

2. System settings ..................................................................................................................... 8

3. Protocol Management ........................................................................................................... 9

4. Reading a Microplate .......................................................................................................... 12

5. Result processing ................................................................................................................ 18

6. Report exporting .................................................................................................................. 26

7. Power off ............................................................................................................................... 27

Chapter 6 Maintenance, storage, transportation ......................................................................... 28

1. Maintenance ......................................................................................................................... 28

2. Storage and transportation ................................................................................................ 28

Chapter 7 Trouble shooting ................................................................................................................. 29

Chapter 8 Accessories ............................................................................................................................ 31

MR9610 Microplate Reader Operation Manual Chapter 1 Brief Introduction

─ 1 ─

Chapter 1 Introduction

The SmartReader™ UV-Vis is an automated microplate reader for ELISA
assays. Through absorbance readings, it can determine the concentration, as
well as positive or negative detection of antibody and antigen in samples when
used with ELISA kits and solutions. The instrument can also be used to test for
purity and quantitate nucleic acids and proteins.

Key Features:

1) 10-inch color touch screen.

2) Operating system allows acquisition, editing and saving of data.

3) Stand-alone system incorporates built-in processing, it can be optionally
connected to a PC with the use of SmartReader software.

4) Graphical interface displays a representative plate for easy setting of well
parameters: blank, sample, standards, positive/negative, quality control
and multi-value comparisons.

5) Dual optical channels, one used for sample detection, and one used for
reference.

6) End-point method, kinetic readings, and spectral scanning can be
performed.

7) Accepts plates with or without lids. Top heating prevents condensation.

8) Xenon lamp offers long life: 109 flashes (1,000,000,000), equivalent to
approximately 1,300,000 plate reads.

9) Plate incubation feature allows accurate and uniform temperature control
(≤0.5°C deviation between wells).

10) Top reading optical path with continual self-checking and automatic
calibration option for monochromator wavelength and plate positioning.

11) Shaking function, time and speed are adjustable.

12) USB flash drive data export, for fast and easy data transfer.

13) Multi-user operation, data management for separate users.

MR9610 Microplate Reader Operation Manual Chapter 2 Features

─ 2 ─

Chapter 2 Features

Working conditions：

Ambient temperature: 10C~40C
Relative humidity: 30%~80% (Non-condensing environment)
Power: AC 100-240V 50-60Hz 2A

Parameters:

Model
Parameter

MR9610

Light source

Xenon flash lamp >109 flashes

Wavelength

200～1000nm

Bandwidth

< 2.5nm

Wavelength accuracy

±2nm

Wavelength
repeatability

0.2nm

Read-out range

0.0-4.0 OD

Linearity@450nm

R2 ≥ 0.999, [0.0 – 3.0]

Accuracy@450nm

1.0% + 0.003 Abs (0 - 2.0]

2.0% (2.0 - 2.5 Abs]
Precision@450nm

CV < 0.5% or SD<0.003 (Precision mode); CV < 1.0%
(Fast mode)

Stability@450nm

< 0.005A , (0.0 - 2.0]
< 2% , (2.0 - 2.5]

Measurement speed

< 8 seconds in Fast mode (96-well plate)
< 28 seconds in Precision mode (96-well plate)

< 74 seconds in Precision mode (384-well plate)

Plate shaking

Linear (Slow: 16.8Hz, 1mm; Medium: 6.68Hz, 3mm;
High: 2.44Hz, 15mm)

Incubation range

RT+ 5°C to 45°C

Temp. uniformity

± 0.5°C @ 37°C

MR9610 Microplate Reader Operation Manual Chapter 2 Features

─ 3 ─

Connections

1 B-type USB port for PC connection
1 Ethernet port
2 A-type of USB ports for mouse and printer connections

Power requirements

Included power adapter: DC24V 6.67A 160W,
AC 100V – 240V

Dimension (W×D×H)

300×500×260mm / 11.8 x 19.7 x 10.25 inch

Weight (kg)

15.5kg / 34 Lbs

MR9610 Microplate Reader Operation Manual Chapter 3 Instrument Structure

─ 4 ─

Chapter 3 Instrument Overview

Structure

Rear cover

Touch screen

Housing

External Buttons

Plate holder

Front door

Extension module

USB Ports

LAN Port

Power Switch

DC Power Inlet

B-type
USB Port

Air outlet

MR9610 Microplate Reader Operation Manual Chapter 4 Installation

─ 5 ─

Chapter 4 Installation

1. Initial check

Each MR9610 is thoroughly tested before shipping, but please check it again when you
receive the instrument and contact your local distributor or Accuris Instruments if:

• The outer package is damaged

• The outer package has any obvious moisture stains

• The outer package has marks of impact

After opening, please check the instrument and box contents.

Confirm that all ordered accessories have been included.
Check the instrument’s appearance for any damage.

2. Installation location and environment

 Installation location: locate the instrument on a flat, dry, clean and stable surface, allow
enough room on the front side so there is space for plate holder drawer to move in and out,
and also keep 15cm / 6 inches of space behind and on the sides for any wires and air flow.
 Environment:

a. The environment for installation should not be excessively humid or dusty.
b. Temperature should be within the range of +10°C ~ +40°C.
c. Relative humidity should be within the range of 30% ~ 80% to avoid condensation.

Note: KEEP THE INSTRUMENT AWAY FROM CORROSIVE GASSES AND
LIQUIDS.

3. Installation steps

① Place the instrument on a stable and level surface.

Note: DO NOT loosen any screws or parts without first consulting with the
Accuris Instruments Service Department or your authorized distributor. Doing
so can cause damage and void the warranty.

② Connect the instrument to an appropriate power outlet using the provided power

cord.

Fig. 1

MR9610 Microplate Reader Operation Manual Chapter 4 Installation

─ 6 ─

Note: One cord coming from the power adapter will fit to the input jack on the
back of the instrument. Make sure to connect it according to the above picture.
The other cord will connect to a standard electrical outlet (100VAC to 230VAC)

③ After connecting the power cords, switch the power button on the rear of the

instrument to the ON position, the front panel will cycle through a start-up and
self-test screen.

Warning: Do not connect the instrument to a power outlet that is not properly
grounded.