User Manual
V1.0
Nucleic Acid
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IsoPure 96
Purification System
I
Thank you for purchasing our IsoPure 96 Nucleic Acid Purification System. This user
BENCHMARK SCIENTIFIC
PO Box 709
Edison, NJ 08818
USA
Phone: 908-769-5555
Website: www.benchmarkscientific.com
/ www.accuris-usa.com
Email: info@benchmarkscientific.com /
info@accuris-usa.com
manual describes the instrument’s features, specifications, as well as complete operating
instructions, please read carefully before operation. Keep this user manual for later use.
Initial Inspection
Please check the instrument as well as all included accessories when you first open the
packaging. If you find anything damaged or missing, please contact Benchmark Scientific
or your local distributor immediately.
Version No.: 1st, November 2018
Test Equipment Depot - 800.517.8431 - 5 Commonwealth Ave, Woburn, MA 01801 TestEquipmentDepot.com
II
Safety Warnings and Guidelines
1. Important information for safe use
Users should have a clear understanding of how to use this instrument
before operation, please read this manual carefully prior to operation.
Any improper operation may cause injured or electric shock.
Please read the manual carefully and operate safely according
to the guidelines.
2. Security
The operation and maintenance and of the instrument should comply with
the basic guidelines and warnings below. Incorrect operation or
maintenance will have effect on using life, performance, and safety
features of the instrument.
The instrument is normal indoor instrument which conforms to
classⅠof GB 4793.1 standard.
Please read this manual carefully before operation. The
device must be used by experienced personnel with
appropriate training.
The operator should not repair the instrument in case any
injury or out-of warranty. If service required, please contact
Benchmark Scientific / Accuris Instruments or your local
distributor for repair.
Before powering on, please make sure the voltage of the power
supply is consistent with the required voltage. And make sure
the rated load of the power outlet is not less than required by
the instrument.
If the power cord is damaged, replace it with the same type and
specification power cord. Do not cover anything on the
instruments when using. Insert and pull the power line with hand
gently and make sure the plug completely insert to the jack.
1
The temperature of the heating block is high, please do not
touch it during the operation in case any injury.
The instrument should be kept in an area with minimal dust,
away from wet areas and direct sunlight. In additional the
installation location should have sufficient ventilation, but away
from electromagnetic interference and heat sources. The vent
on this instrument are designed for ventilation. Do not cover
them in case overheat. When many instruments are used at the
same time, the distance between each instrument should be
more than 100cm.
Power off when not in use. If the instrument will not be used
for a long period of time, cover it with a cloth or plastic to
protect it from dust.
Disconnect the power cord from the jack at once in the
following cases, and contact your local distributor or Benchmark
Scientific / Accuris Instruments:
⚫ Liquid enters into the Instrument;
⚫ Instrument was rained or watered.
⚫ Abnormal operation: such as abnormal sound or smell.
⚫ Instrument dropping or outer shell damaged.
⚫ The function has obviously changed.
3. Maintenance
The instrument should be cleaned regularly using a soft cloth damp with
small amount of alcohol. If any stain on the surface of the instrument, wipe
it with soft cloth damp with cleansing cream.
4. Transportation and storage
requirements Ambient temperature: 10℃ ~
35℃ Relative humidity: ≤70%
Atmosphere pressure range: 500 ~ 1060hpa
Locate it in a well-ventilated room, away from corrosive gas.
2
Contents
Chapter 1 Introduction.................................................................................................................. 1
1. Application .......................................................................................................................................... 1
2. Contraindication .............................................................................................................................. 1
3. Service Life .......................................................................................................................................... 1
Chapter 2 Specifications ............................................................................................................... 2
1. Working Conditions ....................................................................................................................... 2
2. Basic Parameters ............................................................................................................................. 2
3. Overall Dimensions ....................................................................................................................... 3
Chapter 3 Basic Operating Instructions .............................................................................. 4
1. Structures............................................................................................................................................. 4
2. Touch Screen ..................................................................................................................................... 6
Chapter 4 Operations ..................................................................................................................... 7
1. Power Connection .......................................................................................................................... 7
2. Kits Installation ................................................................................................................................ 7
3. Detailed Operations ...................................................................................................................... 8
3.1. Start-up Interface ....................................................................................................................... 8
3.2. Run Program Interface ............................................................................................................ 8
3.3. Manage Program ................................................................................................ ...................... 15
3.4. System Settings..........................................................................................................................19
3.5. UV Sterilization ................................................................................................ .......................... 23
3.6. Help ...................................................................................................................................................24
Chapter 5 Trouble Shooting .....................................................................................................25
1. Troubleshooting ............................................................................................................................25
2. Software Error Alarm List ........................................................................................................26
Chapter 6 Accessory ......................................................................................................................27
Chapter 7 Abbreviations and Tags .......................................................................................28
1. Abbreviations ..................................................................................................................................28
2. Tags ........................................................................................................................................................29
3
IsoPure 96 Operation Manual Chapter 1 Introduction
Chapter 1 Introduction
The IsoPure 96 nucleic acid purification instrument is a newly launched
automatic extraction and purification system for DNA/RNA, proteins and
cells. It can absorb, transfer and release magnetic beads by magnetic rod
and magnetic rod sleeve to separate magnetic beads and samples. The
operation is automatic, fast and simple. Users can extract 1~24, 1~48 or
1~96 samples simultaneously with special kits. Auto pure series can extract
samples of animal/plant tissue, blood and body fluids, etc with different
kinds of magnetic bead nucleic acid extraction reagents. It is mainly used
for the extraction and purification of nucleic acid from human body
samples.
1. Application
This instrument is suitable for the extraction and purification of nucleic
acids in animal and plant tissues, blood and body fluids and other
samples(mainly used in human body samples).
2. Contraindication
No contraindication.
3. Service Life
Service life of the instrument is five years.
For production date, please see the label on back of the instrument.
1
IsoPure 96 Operation Manual Chapter 2 Specifications
Chapter 2 Specifications
1. Working Conditions
Environmental Temperature: 10 C～35 C
Relative Humidity: ≤70%
Input Voltage: AC 100～240V，50Hz/60Hz
2. Basic Parameters
Model
IsoPure Mini
IsoPure 96
Parameters
Principle
Magnetic Particle Method, Magnet type
Sample Volume
200μL-10000μL
50μL—1000μL
Throughput
24
96
Stability
CV≤5%
Extraction time
10 ~ 60min/time
Temperature
Room temperature to 120℃ for lysis and elution
control module
Temp. Accuracy
±1
℃
Vibrateand mix
10 different speeds for option
Operation
7 inch color touch screen, mouse can be connected
Programs
8 groups of programs can be preset, and can store 100
groups of programs
Program
Including create, edit, delete and protocol mode
management
Extension
With USB port and Ethernet port
interface
Network
Extended Ethernet remote control, WiFi function.
Power Supply
AC100-240V, 50Hz/60Hz, 250VA
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IsoPure 96 Operation Manual Chapter 2 Specifications
3. Overall Dimensions
Unit: mm
Fig 1
3
IsoPure 96 Operation Manual Chapter 3 Basic Operating Instructions
Chapter 3 Basic Operating Instructions
This chapter mainly introduces structures, basic operation keys,
displays, as well as preparations before starting up. Please read this
chapter carefully before using this instrument.
1. Structures
1.1. Front
Display
Shortcut keys
and indicator
Cabin door
Fig 2
1.2. Back
Power Switch
USB Port
Screw to remove
Fan
transparent cover
Screw to remove
panel
Power
Socket Fuse
Fig 3
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IsoPure 96 Operation Manual Chapter 3 Basic Operating Instructions
1.3. Cabin Door
The cabin door of IsoPure 96 can be opened which is convenient for
cleaning and maintenance.
Cabin door
Magnetic rod
Magnetic rod’s tip
Heating plate
Fig 4
1.4. Transparent Cover
The transparent cover is on the right side of the instrument which is
for placing or taking out kits.
The cover can be removed which makes it convenient matching with
automatic liquid transfer wrokstation.
Screw for quick removel
Transparent cover
Fig 5
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IsoPure 96 Operation Manual Chapter 3 Basic Operating Instructions
2. Touch Screen
Indicator light
TAB
RUN
Display screen
STOP
Fig 6
Display screen: Touch screen, mouse also can be connected for operation.
TAB: Select shortcut program.
RUN: Start the shortcut program and run the instrument.
STOP: Stop the operation.
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IsoPure 96 Operation Manual Chapter 4 Operations
Chapter 4 Operations
1. Power Connection
AC 100 ~ 240V
2. Kits Installation
Open the cabin door, put kits on the plate position of the rotary table,
press position button to turn the rotary table and place all the kits in turn.
The IsoPure 96 is suitable for 96-well kits.
Position button
Fig 7
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IsoPure 96 Operation Manual Chapter 4 Operations
3. Detailed Operations
3.1. Start-up Interface
Turn on the instrument and make sure the door is closed before start,
start-up interface will comes up.
Fig 8
Then, it will enter into “Run Prog.” interface.
3.2. Run Program Interface
This interface including two modes: “shortcut” mode and “list
mode”, as shown in below Fig 9 and Fig 10.
Fig 9
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 10
In the “List mode” interface, if one program selected/activated in
“Shortcut” column, the icon of the program can be displayed on
shortcut interface. 8pcs of programs can be activated in maximum at
the same time.
“SN”, “Name”, “Modify time” and “Lock”are non-editable options.
3.2.1. Run Interface
In “List mode” or “Shortcut” mode, select required program and
click “Run” to enter into run interface.
When running the program, the instrument will first detect the
presence of the kit on the rotary table. If no kit is found on the board of
the setup program, the program will prompt to confirm whether the
following steps can be continued, as shown in the figure below.
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Test Equipment Depot - 800.517.8431 - 5 Commonwealth Ave, Woburn, MA 01801 TestEquipmentDepot.com
IsoPure 96 Operation Manual Chapter 4 Operations
Fig 11
Fig 12
The instrument will install magnetic rod sleeve automatically. If rod
sleeves are already installed on the current magnetic rod sleeve rack, "Sleeve
loaded, continue?” will pop up. If no magnetic rod sleeve is detected after
installing the magnetic rod sleeve, "No sleeve, continue?" will appear.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 13
Fig 14
After the magnetic rod sleeve is successfully installed, the instrument
automatically performs the following steps, please see Fig 15.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 15
In the running interface, users can stop, pause, continue or run the
program again. The plate with dark blue color, number 1, is the working
plate, the red corner marks on it means the plate is running or already
finished running, while blue corner mark means the plate which is ready
to run, one corner mark means one running and two means two runs. A
corner mark represents the plate position used once in the whole
program.
After the completion of the operation, the No. 8 plate position will
be automatically pushed to the transparent cover on the right side.
3.2.2. View
In the list mode or shortcut mode, select the required program, and
click “View” button to enter the view interface (See Fig 16). Users can
view each parameter settings of the program.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 16
Users can click button in the upper right corner to switch to
the graphic display. Highlight displays the plate position which
corresponding to the selected step, please see Fig 17 as below.
Fig 17
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IsoPure 96 Operation Manual Chapter 4 Operations
Then click button to magnetic parameter absorption interface
which displays magnetic parameters of selected step as shown in Fig 18.
“Steps Run”: run the program starts from currently selected step.
“Option”: view settings of the program, please see Fig 19.
Fig 18
Fig 19
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IsoPure 96 Operation Manual Chapter 4 Operations
3.3. Manage Program
Users can manage all programs in “Manage Prog.” interface.
Fig 20
3.3.1. Management Interface
Management interface is similar to list interface in program
operation, except that locking column is non-operable option in program
run interface while it’s an operable option in management interface. Click
the lock icon to switch lock and unlock. Programs cannot be edited, saved
or deleted if in lock state, please make the change in unlock state.
3.3.2. New/Edit interface
When the users click the “New” or “Edit” button, interface of Fig 23
will appear, the main difference between “New” interface and “Edit”
interface is whether the program name exists or not, other operations
are similar. This interface mainly includes five buttons: “Insert”,
“Delete”, “Option”, “Save” and “Back”.
Insert: click “Insert” to add a new program with default parameters
next to the current selected program, the new program should be with a
valid name.
Delete: delete the selected program.
Option: Option is the high-level parameter setting which applies to
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IsoPure 96 Operation Manual Chapter 4 Operations
the entire program scope.
Save: save the program file, please note a valid program name is
necessary.
Fig 21
“Insert” interface as Fig 22.
Fig 22
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IsoPure 96 Operation Manual Chapter 4 Operations
Plate: select a plate position for the coming operation
Name: set a name of the step
Mix time: the mixing time for selected plate.
Mix amp: mix amplitude, the range is from 1 to 100%.
Wait time: interval time between two steps.
Volume: The volume is automatically converted to the amplitude of
mixing according to the formula.
Mix speed: 10 kinds of mix speeds from 1 to 10. The higher the value is,
the faster the mixing speed will be.
Temp.: The temperature can be set according to actual requirements,
only No.2 and 8 wells can be set.
Click “ ” to enter parameter settings of magnetic absorption, see
below picture please.
Fig 23
Segments: setting range is 0 ~ 5, it can stop to do magnetic absorption
for each segment, magnetization function will be closed if set it to 0.
Cycle times: repeat magnetic absorption times.
Mag.speed: It’s magnetic absorption speed when magnetic rod moves
under the liquid level. 1 is the slowest while 10 the fastest.
Lip-lvl: the standing time when magnetic rods closing to liquid level after
finishing magnetic absorption which is for magnetic beads gathering in
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IsoPure 96 Operation Manual Chapter 4 Operations
case beads falling off due to liquid surface tension. Anti-splash: the
standing time when magnetic rods pulling away from liquid level after
finishing magnetic absorption, in case cross contamination which caused
by liquid splashing due to some special sample tissues falling off.
1-5 Segment time: independent magnetic absorption time of each
segment, the maximum time can reach to 999 seconds.
Estimated: The estimated magnetic absorption time of the software.It
can only be displayed on the next entry after exiting the interface.
3.3.3. Option
In program new or edit interface, click the “Option” to enter the
option interface. The parameters in the option are applied to the whole
program as shown in the figure below.
Fig 24
Confirm: Save all settings and exit.
Back: Not save all settings and exit.
Heating Setup: It is used to set the heating type.
➢ Heating synchronization: It indicates that the heating and
magnetic rod sleeve action are synchronous.
➢ Preheating: It indicates that the heating board will rise to the set
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IsoPure 96 Operation Manual Chapter 4 Operations
temperature first, and then the magnetic rod sleeve frame starts to work.
➢ Start when: It indicates that the magnetic rod sleeve frame starts
to work when the temperature rised to the set temperature which is
lower than the target temperature.
Cooling Setup: It is used to set the cooling
type. 3.3.4. Save As/Delete
In the “Manage prog” interface, click the save as button to save the
file, and click the delete button to delete the file.
3.4. System Settings
In system setting interface, “Instrument”, “Date&time”, “Language”,
“Air ejector fan”, “Im.&export” and “Upgrade” can be modified.
Fig 25
3.4.1. System Time
Click “Date & time” button to enter modification interface, as
shown in the figure below.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 26
The date and time can be adjusted by “+” or “-” buttons.
3.4.2. Language Settings
Two options: Chinese and English.
Fig 27
Select the language , press “Ok “ to save the modification.
3.4.3. Fan
Click “Air Ejector Fan” to choose “On” or “Off”.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 28
3.4.4. Import and Export
Click the “Im.&export” to below interface.
Fig 29
Press the "Import " to enter U disk directory and then select the
program needed, press the "Ok" to import.
Press the "export" button to enter the system directory, select
programs and then "Ok" to export files to the U disk.
3.4.5. Software Upgrade
Click "Upgrade" to upgrade interface, see Fig 30 please.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 30
Insert the U disk with the latest software in, and then upgrade the
interface software or control software of the instrument.
3.4.6. Operation Record
Each run of the program automatically generates a running record.
Fig 31
Users can trace records by “Search” button, see Fig 32 please.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 32
Log exports can be done through the export key.
3.4.7. Lighting
At the rith bottom of the screen, if icon “ ” appears, it means the
lighting is on while lighting is off if the icon displays “ ”. Users can click
the icon to switch between on and off.
3.5. UV Sterilization
The UV disinfection interface is mainly used for the opening and
closing of the UV lamp. The time can be set by pressing “+” or “ -”
button. The program can automatically determine half of the set time to
sterilize the half circle of the rotary table, with a minimum of 2min, as
shown in the figure below.
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IsoPure 96 Operation Manual Chapter 4 Operations
Fig 33
3.6. Help
Help interface displays help information and version as shown in
the figure below.
Fig 34
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IsoPure 96 Operation Manual Chapter 5 Trouble Shooting
Chapter 5
Trouble Shooting
1. Troubleshootings
No.
Symptom
Causes Analysis
Method
Power not connected
Check power
1
No display after switch on
Switch failure
Replace switch
Fuse failure
Replace fuse (5X20 250V 8A)
Others
Contact with Distributor
2
No UV light
UV light failure
Replace light tube
Contact with distributor
3
No light
Light failure
Replace light tube
Contact with distributor
Can not stop
4
automatically after
Sensor failure
Contact with distributor
opening the door.
Big variance between
5
actual and display
Sensor failure
Contact with distributor
temperature
6
No heating for
heating
Sensor failure
Contact with distributor
strip
Heater failure
7
Instrument can’t run
Controller failure
Contact with distributor
Motor failure
Guide rail installed
Abnormal sound
during
incorrect
8
Motor failure
Contact with distributor
working
Synchronous belt
abrasion
9
Press button not working
Press button failure
Contact with distributor
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25
IsoPure 96 Operation Manual Chapter 5 Trouble Shooting
2. Software Error Alarm List
Fault type
Fault name
Error
Temperature
T1 Overheat
E011
(code: 0)
T1 Open circuit
E015
T1 Short circuit
E016
Baffle motor sensor
E404
Rotary motor sensor damaged
E405
Electric machinery
Lifting platform motor sensor damaged
E406
stroke position
(code:4)
Push rod motor sensor damaged
E407
Motor position sensor of magnetic rod sleeve
E425
damaged
Magnetic rod motor position sensor damaged
E415
The clock crystal fault
E702
LCD, Crystal
Memory chip E2P damaged Setting parameters lost
oscillator, Storage
New instrument, instrument type hasn’t been set
(code: 7)
E703
Zero has not been calibrated, the instrument zero
calibration is not in the 3 well will lead to the
program does not working
Communication
Moving parts online failure
E801
(code: 8)
Rotary parts online failure
E802
26
IsoPure 96 Operation Manual
Chapter 6
Accessory
Chapter 6
Accessory
No.
Name
Specs.
Unit
Qty.
Remark
1
Power cord
Pc
1
1
Mouse
Logitech
Pc
1
27
IsoPure 96 Operation Manual
Chapter 7 Abbreviations and
Tags
Chapter 7 Abbreviations and Tags
1. Abbreviations
The following Abbreviations are for reference and will appear in this operation manual.
A
ampere
AC
alternating current
V
volt
Hz
Hertz
W
watt
USB
universal serial bus
SD
secure digital card
WiFi
wireless Fidelity
Kg
kilogram
mm
millimeter
μL
microliter
hpa
hectopascal
℃
degree centigrade
CV
stability
TAB
tab
RUN
run
STOP
stop
28
IsoPure 96 Operation Manual Chapter 7 Abbreviations and
Tags
2. Tags
Following marks appear on the instrument
Warning label
Heating label
CONFORMITE EUROPEENNE
Be careful of hands
29
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Notes
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