Azure c150, c200, c300, c400, c500 User Manual

Azure

cSeries

User Manual

for the c150, c200, c300, c400, c500 and c600

™

NIR RGB CHEMI

Rev 20150428

Azure™ cSeries User Manual Page 1

BLUE

LIGHT

WHITE

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UV

Safety and Regulatory Compliance

Important Safety Information

Please read these instructions before operating the Azure™ cSeries imaging system.

UV Safety Precautions

Because U.V. radiation can cause serious damage

UV

CAUTION

The Azure™ cSeries system comes with a built-in Ultra-Violet (UV 200-400nm) Transilluminator inside
the system. Exposure to UV radiation can cause permanent damage to the eyes and skin. The system

enclosure connes the radiation within the system and shields the user from exposure. The system
is also equipped with a two-way safety interlock switch which automatically cuts o the power to the

transilluminator when the door is open during normal use.

The Azure cSeries imaging systems belongs to Class A equipment, and fullls the limit values of table

3 but not table 4 of EN 61326:1997+A1+A2+A3. It may become necessary to defeat the safety lock or
operate the transilluminator outside the system for service. In this case, be sure to use the following
safety precautions:

• Always wear UV-protected eyewear that is specied by the manufacturer as providing

protection at the wavelength(s) used, making sure that the eyewear protects any areas where
radiation may come through (UV sunglasses may not prevent UV radiation from coming in
through the sides or around the lenses).

to unprotected eys and skin, we recommend to
wear U.V. protection glasses or face shield.

• Always cover all skin that may be exposed to UV light, especially the face, neck, hands, and

arms.

• Always make sure that any UV protection devices (such as the safety switch on the light

cabinet apparatus) are working properly. If not, discontinue use until the device(s) are properly
repaired.

• Please use only UV lamps in the transilluminator.

Electrical Safety Precautions

Be sure to take proper precautions when handling any electrical equipment. NEVER work on any live

circuit, xture, receptacle, or switch. Safety rules you should follow whenever working with any electrical

appliance include:

• Always shut o power at the main disconnect before changing a fuse.

• Always shut o power to the circuit before repairing or replacing a switch, receptacle, or xture.

• Always tape over the main switch, empty fuse socket, or circuit breaker you are working on.

• Always check that the circuit is dead before beginning work on it. Using a circuit tester or

voltmeter can help you determine this.

• Always unplug any appliance before repairing it.

Protective earth terminal

Azure™ cSeries User Manual Page 1

The earth terminal, intended for connection to an external protective conductor for protection against
electric shock in case of a fault, is located on the inside of the back panel.

Hot surface warning

Under normal conditions, the temperature of glass surface of UV transilluminator is below

50 °C and safe to touch. However, if the system malfunctions, it is possible that the
glass surface temperature exceeds 80°C. Please exercise caution when touch the glass
surface with hand when this occurs.

Laser Safety

General Information

Azure Biosystems imaging system models c600 and c500 both include a laser illumination system that
provides 660nm and 785nm Narrow band excitation for NIR applications. The imaging systems are

certied to comply with CE, UL and CSA safety standards and consists of two fully enclosed electronic

modules located on the right and left interior sides of the imaging chamber. This laser system is by its

appropriate classication and denition a non-removable laser system as it is not operable when the

laser modules themselves are removed from within the system.

Safety Features

Azure Biosystems imaging systems are designed to prevent direct and collateral human exposure to
laser radiation by means of a safety interlock switch located on the right front side of the imaging
chamber. The safety interlock reacts to “door open” and “door closed” states and defeats all power

to internal light sources when the door is in the “open position”. Lasers and other internal system

light sources will not power on unless the door is fully closed. If the access door is opened during

imaging, all light sources will immediately power o to prevent human exposure to internal illumination

sources. Right and left laser modules located in the c500 and c600 imaging chamber are installed in a

highly diused protective metal enclosure preventing exposure to full laser radiation power. In addition,

the entire laser system is fully enclosed within the system enclosure and there are no viewing ports,

windows, or openings to facilitate viewing of, or exposure to laser radiation elds from direct impact,
reection, or leakage.

Maintenance

Azure Biosystems c500 and c600 laser systems do not require regular, periodic, or preventive maintenance
in the form of adjustments, calibrations, cleaning, or other standard maintenance procedures to maintain
optimal performance, thereby removing the need for users or their service technicians to initiate any
actions where exposure to laser radiation would occur.

Serviceability

Replacement of faulty laser modules is a manufacturer-only repair action and not a customer-service

action. Laser repair or replacement may be performed in the eld by Azure Biosystems authorized

service technicians, or by return of the entire system to Azure Biosystems, or its authorized service

location(s) for laser repair or replacement. Lasers are deemed to be faulty or defective if users discover
images that show evidence of output signal level loss in either the left or right laser module, a signicant
dierence between the output signal levels between the left and right lasers modules, or complete loss

of output signal level in either or both laser modules. Users or their service technicians should make no
attempt to determine the cause of faulty laser operation, and should promptly contact Azure Biosystems
at [e-mail] or their nearest Azure Biosystems authorized service location.

Azure™ cSeries User Manual Page 2

Caution

Azure Biosystems c500 and c600 systems contain a defeatable safety interlock system. It is not
recommend or advised by Azure Biosystems, under any circumstances, for users to defeat the interlock
system and perform laser imaging, or imaging with any c500 or c600 light source with the access door
open. The access door must be fully closed.

For Research Use Only

This instrument is suitable for research use only. It must be used, therefore, only by personnel who know
the health risks associated with the reagents that are normally used with this instrument.

Warranty

The Azure cSeries products are warranted against defects in materials and workmanship for one year
unless otherwise outlined on your sales order or www.azurebiosystems.com/warranty. If any defect
occurs in the instrument during this warranty period, Azure Biosystems, Inc. will repair or replace the

defective parts at its discretion without charge. The following defects, however, are specically excluded:

• Defects caused by improper operation.

• Repair or modication done by anyone other than Azure Biosystems or the company’s

authorized agent.

• Use of spare parts supplied by anyone other than Azure Biosystems.

• Damage caused by accident or misuse.

• Damage caused by disaster.

• Corrosion caused by improper solvents or samples.

Voltage Setting Information

The Azure cSeries imaging system has a power supply that automatically chooses the correct voltage
for your country or region.

CE Conformity

The following Azure™ Imaging Systems, models: c150, c200, c300, c400, c500, c600 are in conformity

with the provisions of the following EC Directives, including all amendments, and national legislation

implementing these directives:

• Low Voltage Directive 2006/95/EC

• EMC Directive 2004/108/EC

And that the following harmonized standards have been applied:

• EN61010-1: 2001

• EN61326-1: 1997+A1:1998+A2:2001+A3:2003 Class A

• EN61000-4-2, EN61000-4-3, EN61000-4-4, EN61000-4-5, EN61000-4-6, EN61000-4-11

Protection category: IP20 according IEC 60529

Contact

Azure Biosystems, Inc.

6773 Sierra Court, Unit B • Dublin, CA 94568 • USA
[e-mail] • [phone] • Fax [phone]

Azure™ cSeries User Manual Page 3

Table of Contents

1. Introduction 5

Table of Specications 6

2. Installation and Setup 6

2.1 System Placement 6

2.2 Connecting to Power 6

2.3 Power On/O the System 6

2.4 Software Installation 7

2.5 Attach Other USB Input Devices to the System 7

3. cSeries Capture Software Overview 7

3.1 Gallery 7

3.2 Gel Applications 9

3.2

3.2

3.3 Chemiluminescent Applications (c300 systems and above) 10

3.3

3.4 RGB Applications (c400 and c600) 11

3.5 NIR Applications (c500 and c600) 12

3.6 Custom Applications 13

3.7 Save Acquired Image 13

A Imaging Workow 9

B Band Excision and Light Safety Switch 10

A Imaging Workow for Chemiluminescent Applications 11

4. Settings 14

5. Help 14

6. Quit 14

7. Tablet and Windows® Tools 14

Tablet Specications 14

7.1 How to Power O/Restart the Tablet 15

7.2 How to Access the Keypad 15

Appendix A: Installing a New Version of the cSeries Capture Software 16

Azure™ cSeries User Manual Page 4

1. Introduction

The Azure cSeries imaging systems oer

high performance imaging for a wide
variety of applications. The cSeries family
of instruments includes the follow products:
c150, c200, c300, c400, c500 and c600.

c600

c500

Select which one ts your applications now,

and learn more about upgrading later when
your needs change.

c400

c300

Each Azure cSeries imaging workstation

can be upgraded in the eld to a higher

model with a wider range of applications,
with the exception of the c150 which
is not upgradeable. Contact us at

[e-mail] to learn more.

The Azure cSeries system includes the following key components:

• Cabinet – The cabinet is a light tight image station.

• Camera – High resolution camera, preinstalled in the system. A motorized lens is included on

the c300 systems and above.

• UV Transilluminator – The UV illuminator provides 302nm and 365nm trans illumination. It is
mounted on a pull out tray.

• EPI Blue and White LEDs – The cabinet also contains EPI white and blue light panels on each
side of the cabinet interior walls.

c200

c150

• EPI RGB LEDs – High intensity LED illuminators (c400 and c600).

• EPI 660nm & 785nm Laser Diodes (c500 and c600).

• Filters – For each light source, appropriate lters are also included in the motorized lter wheel.

• Tablet PC – The tablet PC is a self contained touch screen PC for system control and image

acquisition.

• Orange Tray – For Visible dyes, the orange tray converts UV light into Visible light.

• Blot Tray – Stored in the cSeries door, the Blot Tray is required for imaging all blots. For
uorescent blots, it is placed on the UV transilluminator. For Chemi blots, it can be placed on

the UV transilluminator, or on the chemi shelf in the cabinet.

• USB Memory Stick – The Azure Biosystems memory stick contains les relevant to your
imaging system.

• AzureSpot Analysis Software – Please contact our technical support to receive your
electronical license at [e-mail].

Azure™ cSeries User Manual Page 5