ROXY
Installation Guide
210.7011, Edition 5, 2018
T +31 71 5813333 | info@AntecScientific.com | www.AntecScientific.com
Antec Scientific
Industrieweg 12
2382 NV Zoeterwoude
The Netherlands
Copyright ©2018, Antec Scientific, The Netherlands. Contents of this publication may not be reproduced in any form or
by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement
and written consent from the copyright of the owner. Antec reserves the right to make changes to the design and specifications of the instrument and this manual without prior notice.
ROXY, ALEXYS, DECADE, DECADE II, DECADE Elite, DECADE Lite, INTRO, Flexcell, ReactorCell, µ-PrepCell,
ISAAC, HyREF, SenCell and SynthesisCell are trademarks of Antec. Whatman™ (word and device) and Whatrnan™
(word only) are trademarks of Whatman lnternational Ltd. SOLVENT IFD™ and AQUEOUS IFD™ are trademarks of
Arbor Technologies, Inc. Clarity®, DataApex® are trademarks of DataApex Ltd. Microsoft® and Windows™ are trade-
marks of Microsoft Corporation. Excel is a registered trademark of the Microsoft Corporation. All other trademarks are
the property of their respective owners.
The software and the information provided herein is believed to be reliable. Antec shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, performance, or use of software or this manual. All use of the software shall be entirely at the user’s own risk
Installation guide 3 (of 33)
Table of contents
Warning Symbols .................................................................................... 4
Intended use ........................................................................................... 6
WEEE directive ....................................................................................... 7
Warranty, spare parts and service .......................................................... 8
Safety Instructions ................................................................................... 8
Introduction .............................................................................................. 10
Preparing for installation ........................................................................ 11
Pre-installation preparations ................................................................. 11
IQ and OQ ............................................................................................. 11
PQ ......................................................................................................... 11
Relevant documents for installation ...................................................... 13
Software download ................................................................................ 13
Contents of ROXY ................................................................................. 14
Installation ................................................................................................ 15
Electrical connections ........................................................................... 16
Software installation .............................................................................. 18
Installation of a flow cell ........................................................................ 20
Installation of grounding kit ................................................................... 22
Performing an experiment ..................................................................... 22
PQ Oxidative mode (metabolite synthesis) .......................................... 25
PQ procedure ........................................................................................ 25
Results .................................................................................................. 26
PQ Reductive mode (disulfide bond reduction) ................................... 27
PQ procedure TiBlue electrode (DC mode) .......................................... 27
PQ procedure Ti electrode (Pulse mode) ............................................. 28
Results .................................................................................................. 29
Trouble shooting ..................................................................................... 30
Dialogue Elite ........................................................................................... 32
Hands-on training .................................................................................. 32
4 (of 33) ROXY installation guide, ed. 4
Warning Symbols
The following symbols are used in this guide:
This sign warns about the risk of electric shock. It calls
attention to a procedure or practice which, if not adhered
to, could result personal injury or even loss of life by electrocution. Do not proceed beyond a danger sign until the
indicated conditions are fully understood and met.
The warning sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could result in severe injury, loss of life or damage or destruction
of parts or all of the equipment. Do not proceed beyond a
warning sign until the indicated conditions are fully understood and met.
The caution sign denotes a hazard. It calls attention to a
procedure or practice which, if not adhered to, could result in damage or destruction of parts or all of the equipment and/or erratic results. Do not proceed beyond a
cautions sign until the indicated conditions are fully understood and met.
The biohazard sign draws attention to the fact that use
of biological materials, viral samples may carry a significant health risk.
The toxic hazard sign draws attention to the fact that use
of toxic solvents or samples may carry a significant
health risk.
Installation guide 5 (of 33)
The attention sign signals relevant information. Read this
information.
The note sign signals additional information. It provides
advice or a suggestion that may support you in using the
equipment.
6 (of 33) ROXY installation guide, ed. 4
Intended use
The ROXY EC(/LC) system or ROXY potentiostat for controlled REDOX
reactions up-front Mass Spectrometric detection. It can be used in a wide
range of application, for example:
• Fast synthesis of metabolites (µ-preparative)
• Rapid risk assessments of drug-protein binding
• Signal enhancement in MS
• Electrochemical cleavage of proteins/peptides
• Reduction of disulfide bonds in proteins/peptides
• Oxidative stress/damage of proteins, DNA, lipids, etc.
Operation of the ROXY EC(/LC) system can involve the use of hazardous
materials including corrosive fluids and flammable liquids. The system
should only be operated by users with the following expertise:
• Completed degree as chemical laboratory technician or
comparable vocational training.
• Fundamental knowledge of liquid chromatography & mass
spectrometry
• Participation in an installation of the system performed by the
manufacturer or a company authorized by the manufacturer
and suitable training on the system, flow cell and control software.
• Knowledge and experience in the safe handling of toxic and
corrosive chemicals and knowledge of the application of fire
prevention measures prescribed for laboratories.
Information on safety practices is provided with your equipment operation
manuals. Before using your equipment or accessories, you must thoroughly read these safety practices. This manual is written for laboratory
technicians and scientists skilled in the art.
For research purposes only. While clinical applications may be shown,
this instrument is not tested by the manufacturer to comply with the In
Vitro Diagnostics Directive.
Installation guide 7 (of 33)
The ROXY EC system is ROHS compliant and in conformity with Directive
2011/65/EU Restricted use of Hazardous Substances in electrical and
electronic Equipment (ROHS).
Antec Leyden B.V. is an ISO 9001 certified company.
WEEE directive
All equipment of Antec Leyden which are subjected to the WEEE directive
shipped after August 13, 2005 are compliant with the WEEE marking re-
quirements. Such products are labelled with the “crossed out wheelie”, de-
picted on the left site.
The symbol on the product indicates that the product must not be
disposed as unsorted municipality waste. Please take care of proper
disposal and recycling.
If needed Antec offers the possibility for recycling. In that case
please ship the instrument back to the manufacturer together with a
decontamination form (Antec Leyden, the Netherlands). They will take
care of the proper disposal and recycling of the instrument.
Manufacturer shipping address for the end-of-life products:
Antec Leyden B.V.
Industrieweg 12
2382NV Zoeterwoude
The Netherlands
In case of questions, or if further information is required about the collection & recycling procedure, please contact your local distributor.
Unskilled, improper, or careless use of this equipment can create fire
hazards, or other hazards which can cause death, serious injury to
personnel, or severe damage to equipment and property. Observe all
relevant safety practices at all times. Only use the device for
applications that fall within the scope of the specified intended use.
Else the protective and safety equipment of the device could fail.
8 (of 33) ROXY installation guide, ed. 4
Warranty, spare parts and service
The warranty period of this ROXY EC system is 1 year on workmanship,
wear and tear parts are excluded. Manufacturer provides operational spare
parts of the instrument and current accessories for a period of five years
after shipment of the final production run of the flow cell. Spare parts will
be available after this five years period on an ‘as available’ basis.
Manufacturer provides a variety of services to support her customers after
warranty expiration. Repair service can be provided on a time and material
basis. Contact your local supplier for servicing. Technical support and
training can be provided by qualified chemists on both contractual and asneeded basis.
Safety Instructions
Adhere to the following guidelines when using the ROXY EC system. The
safety practices are intended to ensure safe operation of the flow cell.
Working environment & safety
The intended use of the ROXY EC system is to perform controlled REDOX
reactions of target compounds (in a suitable liquid electrolyte medium) upfront Mass Spectrometric detection. Operators using the system should
have the appropriate education an extensive understanding of GLP rules
and be skilled in the art. Use this system ONLY for the intended use. Use
of the system for any other purpose might cause unsafe situations.
Operation
To assure optimal performance it is recommend that the ROXY EC system
is checked regularly and maintenance procedures are carried out. Preventive maintenance contracts are available for that Purpose. Please contact
your local dealer or the nearest sales office for more information.
Solvents
The solvents used may be flammable, toxic or corrosive. The room in
which the system is installed should be well ventilated to prevent that solvent vapors cause poisoning or ignite and cause a fire. Use of open fire in
the vicinity of this system must be strictly prohibited. Do not install the system in the same room with any other equipment that emits or could potentially emit sparks. Provide protective equipment near the instrument, when
solvent gets into the eyes or on the skin, it must be flushed away immediately. Provide equipment, such eye wash stations and safety showers, as
close to system as possible. Use proper eye and skin protection when
working with solvents. Additional safety requirements or protection may be
Installation guide 9 (of 33)
necessary depending on the chemicals used in combination with this
equipment. Make sure that you understand the hazards associated with
the chemicals used and take appropriate measures with regards to safety
and protection. Sample containers (vials) should be sealed to minimize any
risks related to solvent vapor.
Biological Hazard
When you analyze biological fluids you need to take the necessary precautions and treat all specimens as potentially infectious. Always wear protective and gloves when handling toxic or biologically infectious samples to
prevent biohazards or hazards while working with the system. When the
system or parts of it needs to be shipped to Antec for service/repair or disposal, it has to be accompanied with a decontamination form which should
be completely filled in and signed by the customer. Without this decontamination form the system or parts of it will not be processed by Antec (either
repaired or disposed).
Waste disposal
Perform periodic leak checks on LC tubing and connections. Do not close
or block the drain in the oven compartment. Do not allow flammable and/or
toxic solvents to accumulate. Follow a regulated, approved waste disposal
program. Never dispose of flammable and/ toxic solvents through the municipal sewage system.
Using the ROXY EC system in other ways than indicated in the manual
might result in erratic or unsafe operation.
10 (of 33) ROXY installation guide, ed. 4
C H A P T E R 1
Introduction
To assure an efficient and successful onsite installation of the ROXY EC
system, we have written this guide. This documents describes all the steps
that should be taken. It contains references to relevant user manuals and
qualification documents.
There are a few different configurations applied for electrochemistry coupled to MS (EC/MS). The EC reactor can be used for continuous flow experiment, or flow injection analysis both are not using chromatography.
Sometimes a secondary solvent is added to study post EC reaction conjugation with glutathione for example. In combination with HPLC the reactor
can be placed prior to the HPLC injector (EC/HPLC/MS) or post column
(HPLC/EC/MS).
This document primarily outlines the ROXY EC/MS installation, for the
other configurations most of the described procedure is the same. Except
that for installation of a autosampler or pump the corresponding user manuals should be used.
Fig. 1. ROXY EC/MS with syringe pump for continuous flow experiments.
This document is intended as a general check list to guide you through
the installation of a ROXY EC system and by no means a replacement
of the installation sections in the individual user manuals supplied with
the system.
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