Agilent Quantitative Screening of Multiresidue Application Note

Application Note

Food Testing and

Agriculture

Quantitative Screening of Multiresidue
Veterinary Drugs in Milk and Egg
Using the Agilent 6495C Triple
Quadrupole LC/MS

Authors

Siji Joseph, Aimei Zou,

LimianZhao, Patrick Batoon,

and Chee Sian Gan
Agilent Technologies, Inc.

Abstract

This application note demonstrates the use of the Agilent Comprehensive

Veterinary Drug dMRM Solution for the screening of 210 target residues in milk and
eggmatrices. The workflow specifies conditions for chromatographic separation,

MS detection, and data processing, using a slightly modified sample preparation

procedure. Workflow performance was assessed based on limit of detection (LOD),
limit of quantitation (LOQ), calibration curve linearity, accuracy, precision, recovery,
and repeatability. Over 93% of veterinary drugs showed LOD of ≤1 μg/kg in milk
samples. Calibration curves for all targets ranged from the LOQ to 100 μg/kg with
a coefficient of correlation (R2) ≥0.99. Target peak area response (%RSD) was
<15%, and retention time (RT) %RSD was <0.5%. Method accuracy values, based on
matrix-matched calibration were within 87 to 117%. The average recovery of 95%
of targets was within 60 to 120%, with repeatability %RSD of ≤15%. Both milk and
egg matrices showed similar quantitative results. Injection-to-injection robustness
results demonstrated excellent target peak area and RT reproducibility across
400injections, confirming the workflow capability for routine multiresidue screening
with large-scale sample sets.

Introduction

The Agilent Comprehensive Veterinary
Drug dMRM Solution is an end-to-end

workflow solution for targeted screening

or quantitation of 210 veterinary drug
residues in animal origin matrices,

which accelerates and simplifies routine
laboratory testing. The solution includes

comprehensive sample preparation,
chromatographic separations,
optimized MS detection method
conditions, data analysis methods, and
reporting templates for 210 veterinary
drugs in various food matrices. The
Comprehensive Veterinary Drug dMRM
Solution minimizes method development

time and combines multiple food
matrix analyses into one easy-to-follow

protocol. Agilent MassHunter data

acquisition software, together with
the dMRM database offers easy
customization of dMRM submethods
based on preferred target list or
regulation, as determined by the user.
The solution is available and has been
verified with two mass spectrometers
(Agilent 6470 triple quadrupole LC/MS
and the Agilent 6495C triple quadrupole
LC/MS) to address diverse sensitivity
demands based on the choice of sample

matrix and specific regulations that

varyglobally.

The solution was originally developed

for the quantitative screening of

210multiclass veterinary drugs in
chick, beef, and pork.1 It was then
demonstrated to be effective for

seafood using salmon and shrimp as
example matrices.2 This study further

demonstrates the applicability for
milk and eggs using the 6495C triple
quadrupole LC/MS. For the 210 target
analytes screened in this study, 103

of them had maximum residue limits

(MRL) established in milk regulated
by the AOAC3—with an additional
16targets regulated by US FDA-CFR4,

US FSIS5, or EU6 regulations/guidelines.

The MRL values are typically lower in
milk compared to meat and seafood,

thus requiring a higher MS detection
sensitivity. Additionally, the high fat

and protein content in milk demands

effective sample preparation and a
sensitive detector to monitor trace levels

of drug residues. Compared to milk, the
number of MRL-established targets for
the egg matrix is fewer and the residue

limits are more relaxed.

Experimental

Standards and reagents

Veterinary drug standards were
purchased from Sigma-Aldrich (St. Louis,
MO, USA), Toronto Research Chemicals
(Ontario, Canada), and Alta Scientific
(Tianjin, China). Agilent LC/MS-grade
acetonitrile (ACN, partnumber
5191-4496), methanol (MeOH,
partnumber 5191-4497), and water
(part number 5191-4498) were used

for the study. All other solvents used

were HPLC-grade from Sigma-Aldrich.
LC/MS additives for mobile phases were

also purchased from Sigma-Aldrich.

Individual stock solutions of veterinary
drugs were prepared from powdered or

liquid veterinary drug standards at 1,000

or 2,000 µg/mL using an appropriate
solvent (MeOH, dimethyl sulfoxide
(DMSO), ACN, or water or solvent
mixture). A few stock standard solutions
(100 µg/mL) were obtained from the
suppliers listed above.

A comprehensive standard mix

(1µg/mL of each target analyte in
50/50 ACN/water) was prepared from
individual stock solutions and used for

this experiment.

Sample preparation

Milk and egg samples were purchased

from a local grocery. For the analysis

of milk, a 2.0 ±0.1 mL portion of milk
was transferred in a 50 mL conical
polypropylene tube. For the analysis

of egg, a 2.0 ±0.1 g portion of the
homogenized sample was weighed in a
50 mL conical polypropylene tube. If not
analyzed immediately, the samples were

stored at –20 °C.

Sample preparation was performed

as per the procedure defined in the
Comprehensive Veterinary Drug

dMRM Solution (G5368AA) using
solvent extraction followed by
Agilent Captiva EMR—Lipid cleanup
(partnumber5190-1003), aided by

the Agilent positive pressure manifold

processor (PPM-48, part number
5191-4101).7 The sample preparation
procedure is summarized in Figure1. The
aqueous extraction step was modified

to adjust the target dilution due to

increased water content in milk andegg.

The following deviations from the

protocol defined in the Comprehensive
Veterinary Drug dMRM Solution
are recommended for the aqueous
extraction step:

– Milk: Concentration of EDTA solution:

1 M, volume added: 200 µL.

– Egg: Concentration of EDTA solution:

0.1M (same as workflow guide),

volume added: 1 mL

Matrix-spiked (pre-extraction) QC
samples were prepared by spiking the

appropriate veterinary standard solution

into the milk and egg matrices at various
levels: 1 μg/kg for low-range QC (LQC),
10 μg/kg for mid-range QC (MQC), and
25 μg/kg for high-range QC (HQC),
respectively. An additional QC level lower
than the LQC of 0.1 μg/kg (LLQC) was
included in the milk analysis to verify
the analytical characteristics of a few
targets, and to meet the very low MRL
requirement. After spiking standards, the
samples were vortexed for 30seconds,
then equilibrated for 15 to 20 minutes
to allow the spiked standards to
infiltrate the sample matrix before
sampleextraction.

2

Matrix-matched calibration standards

Two 50 mL
tubes 2.0 g
sample

for QC samples

Blank matrix eluent

Matrix-matched (postextraction)
calibration standards were prepared
as per the workflow protocol by
spiking appropriate standards into the
blank matrix extract.7 The targeted

concentrations of matrix-matched

calibration levels were 0.1, 0.25, 0.5, 1.0,

2.5, 5.0, 10.0, 25.0, 50.0, and 100.0 μg/kg
(10 levels). An additional matrix-matched
calibration level of 0.05μg/kg was
added for milk analysis for similar
consideration of few targets with very
low MRL requirement. Considering the

10x dilution factor introduced during

sample preparation, the actual spiking

concentrations of postextraction

calibration standards were 0.005, 0.01,

0.025, 0.05, 0.10, 0.25, 0.5, 1.0, 2.5, 5.0,

and 10.0 μg/L (ppb) in the milk blank

matrix extract.

Neat standards at 2.5 μg/L in a 50/50
ratio of ACN/water was used to
evaluate matrix effects by comparing

the responses in the corresponding

postextraction-spiked calibration

standards.

Chromatographic separation was
performed using an Agilent InfinityLab
Poroshell 120 EC-C18 column
(partnumber 695575-302) installed on
an Agilent 1290 Infinity II LC, including

Agilent 1290 Infinity II flexible pump
(G7104C), Agilent 1290 Infinity II
multisampler (G7167A), and Agilent1290

Infinity II multicolumn thermostat

(G7116A).

Mobile phase A was water with 4.5 mM

ammonium formate, 0.5 mM ammonium

fluoride, and 0.1% formic acid; mobile
phase B was 50/50 ACN/MeOH with

4.5 mM ammonium formate, 0.5 mM

ammonium fluoride, and 0.1% formic
acid. The LC system was equipped with
a 20 µL injection loop and multiwash
capability. Please see the workflow guide
included with the Agilent Comprehensive

Veterinary Drug dMRM Solution

(G5368AA) for additional details.

7

The “6495 Veterinary Drug

Comprehensive” method included in the
Comprehensive Veterinary Drug dMRM

Solution for the 6495C triple quadrupole
LC/MS (G6495C) was used directly for
acquisition. The 6495C LC/MS triple
quadrupole with an Agilent Jet Stream
(AJS) ion source was operated in
dynamic MRM (dMRM) mode. Autotune
was performed in unit resolution with
report m/z below 100 mode enabled.
MassHunter acquisition software version

10.0 was used for data acquisition,

and MassHunter quantitative analysis

software version 10.0 was used to

process the data.

Results and discussion

Workflow performance in milk

Chromatographic separation using the

Agilent InfinityLab Poroshell EC-C18

column resulted in good separation and

RT distribution of 210 veterinary drugs
within a 13-minute elution window.

Target-specific MRM transitions
included in the dynamic MRM method
helped to meet regulatory requirements
for compound identification and
confirmation. The default dynamic
MRM method utilized a cycle time of

750ms, and dwell times for each dMRM
transition ranged from 7 to 370ms,

offering more than 10 data points

across any given peaks. Figure2 shows

a representative MRM chromatogram
for all 210 veterinary drug targets,

postextraction spiked at 1.0 μg/L in the
milk blank matrix extract. Considering

the dilution factor during sample

preparation was 10x, this 1.0 μg/L
postextraction spike was equivalent to a
10 μg/kg spike in milk. The symmetrically
sharp peaks demonstrate the efficient

chromatographic separation of targets

within the elution window. Table 1 lists
the name, chemical class, CAS number,

and RT of all 210 targets covered in

thiswork.

1

2

1

Figure 1. Sample extraction procedure using solvent extraction followed with Agilent Captiva EMR—Lipid cleanup.

Blank matrix

(no spike)

2

Pre-extraction spike

2-Step solvent

extraction

Centrifuge

Sample cleanup using

Agilent Captiva EMR—Lipid 3 mL

cartridges on Agilent PPM-48

Homogenized

eluents

for matrix blank

and post-extraction

spike calibration levels

LC/MS analysis using

Agilent 6495C TQ

Pre-extraction spike

eluents as QC samples

3

×10

5

Acquisition time (min)

Counts

Acquisition time (min)

Counts

2.0

1.5

1.0

0.5

0

1 2 3 4 5 6 7 8 9 10 11 12

Figure 2. Representative MRM chromatogram of 210 veterinary drug targets postextraction spiked at 1.0 μg/L in the milk
blank matrix extract using the Agilent 6495C triple quadrupole LC/MS).

From the AOAC MRL established

list, the early eluted analytes,

including amoxicillin, baquiloprim,
deacetylcefapirin, diminazene, imidocarb,

norgestomet, sulfaguanidine, and

tilmicosin showed split peaks due

to solvent effects. The “spectrum

summation” integrator algorithm was
used to reliably and automatically

integrate these targets for consistent RT,
and thus eliminated the need for manual
reintegrations.8 The peak shape for these

targets can be improved by converting

samples into a higher aqueous mixture

prior to LC/TQ injection.

LOD, LOQ and calibration

curvelinearity

LOD and LOQ were established using
various low level matrix-matched
calibration standards.

1,2

The

signal-to-noise ratio (S/N) was calculated
using the peak height for signal and an

auto-RMS algorithm for noise, included
in the MassHunter quantitative analysis

software. The method sensitivity using
the 6495C LC/TQ system offered a
LOD ≤1 μg/kg for over 93% of analytes
tested in both milk and egg. The low
detection limits achieved allowed the

high sensitivity demand for screening
trace level veterinary drug residues in

milk. As an example, AOAC regulated

MRL of 0.05μg/kg for clenbuterol in milk.
The 6495C TQ-based workflow provided
a clean, symmetrical peak with S/N of

calibration level, thus enabling confident

target identification and quantitation

(Figure 3).

32 at the 0.05 μg/kg matrix-matched

2

×10

RT: 5.42 (min)

5

4

3

2

1

0

5.1 5.2 5.3 5.4 5.5 5.6 5.7

Figure 3. MRM chromatogram of clenbuterol (MRM 277.1 & 202.9)
postextraction-spiked at 0.005 μg/L (black trace) and 0.01 μg/L (blue trace) in
the milk matrix extract, overlaid with matrix blank (red trace). The defined LOD
of clenbuterol is 0.05 μg/kg (S/N: 32) and LOQ is 0.1 μg/kg (S/N: 76).

4

A calibration curve for each target

Acquisition time (min)

Counts

was generated using matrix-matched
calibration standards at levels
ranging from the defined LOQ to
the highest-spiked level. The linear
regression was used with ignored
origin and 1/x or 1/x2 weight. All targets
met the calibration curve linearity

requirement of R2 ≥0.99. The LOD, LOQ,

and calibration curve data of all targets in
the milk are shown in Table1.

Instrument method precision

andaccuracy

Precision was determined by calculating
the %RSD of the target response and

RT using triplicate injections of the

matrix-matched calibration levels.

The average accuracy value for each

matrix-matched calibration level was also
calculated from the triplicateinjections.

Good precision and accuracy values

were obtained for all targets in milk.
Target response %RSD for all targets in
the milk matrix at 10 μg/kg was <15%,
and the RT %RSD of all targets were
within 0.5%.9 The accuracy values of
all targets at 10 µg/kg within a range
of 87 to 117%. These results confirm
the reproducibility of chromatographic

separation and MS detection.

average recovery was calculated from

duplicate injections of four technical

preparations. The intrabatch recovery
repeatability was measured as %RSD

of recovery, calculated using four

technical preparations of matrix-spiked
QCsamples.

The results showed that recoveries of
about 93% of MRL-established targets
reached the acceptable range of 60
to 120% with an excellent intrabatch
RSD ≤20%.9 Recoveries of the
remaining seven targets, baquiloprim,

chlortetracycline, deacetylcefapirin,

diclofenac, imidocarb, oxytetracycline,
and trichlorfon [DEP], were within
a range of 30 to <60% or >120 to
124%. However, for these targets, the
workflow still provided good recovery
repeatability values within a %RSD of

2

×10

7

6

5

RT: 5.40 (min)

9%, demonstrating consistent extraction
behavior. These results confirmed
the entire workflow reproducibility
using Captiva EMR—Lipid sample

extraction and cleanup protocol in the

6495-TQ-based instrument detection.
The recovery and repeatability results
of all 210 targets are included in Table 1
(see Appendix).

The workflow performance combined
with the 6495C LC/TQ detection helped
confident recovery and repeatability
assessment at trace levels in milk.
Figure 4 shows an example of workflow
recovery and repeatability for clenbuterol
at 0.1 μg/kg in milk. The average
recovery of this target using the LLQC
sample is 118% with good recovery
repeatability of %RSD <5%.

Target recovery and
intrabatchrepeatability

The impact of sample preparation on

target recovery was assessed using
matrix-spiked QC samples. Each QC
level was prepared with four technical
preparations and was injected for

instrument analysis in duplicates. An

appropriate level of matrix-spiked QC
sample based on MRL was selected

to evaluate target-specific recovery

and repeatability. Recovery was

calculated using target response in

matrix-spiked QCs, and measured

response using matrix-matched

calibration curve equations. The

4

3

2

1

0

5.1 5.2 5.3 5.4 5.5 5.6 5.7

Figure 4. MRM chromatograms of clenbuterol (MRM 277.1 & 202.9) using
four technical preparations of LLQC samples in milk (green traces) overlaid
with matrix blank (red trace).

5

Matrix effect assessment

Matrix effect (ME) is an important

parameter for method sensitivity and

reliability assessments. ME is defined as
the ratio of analyte area response (I) in
matrix-matched samples with those in
the corresponding neat standards (see
Equation1). The closer the ME value is to
100%, the less the matrix effect presents;
results lower than 100% indicate matrix
suppression, while results >100%

indicate potential enhancement.

Equation 1.

I

matrix

I

solvent

× 100

ME =

In this study, ME was investigated

using target response from

postextraction-spiked calibration
levels at 2.5 μg/L in blank matrix
extract, compared with corresponding
neatstandards.

In the milk matrix, within a total of
103 MRL established analytes, >95%
of targets showed an ME of >75%,
indicating negligible matrix suppression.
Four targets (amoxicillin, cefalonium,
nafcillin, and sulfamethizole) resulted in
an ME of 50 to 75%, indicating moderate

ion suppression. Target deacetylcefapirin

showed an ME of 48%, indicating

significant ion suppression.

Method verification in egg matrix

The method sensitivity in the egg was
similar to that of the milk matrix. Linear
matrix-matched calibration curves
ranging from LOQ to 100 μg/kg were
demonstrated with R2 ≥ 0.99. Instrument
method precision (%RSD) for target
responses and RTs were <15% and
<0.5%, respectively. Instrument method

accuracy for the matrix-matched

calibration level at 10.0 μg/kg were within
80 to 113% (n = 3). Recoveries of over
94% targets in the egg matrix were within
60 to 120% acceptance criteria, and
recovery repeatability with %RSD values
≤15%. Targets amprolium, baquiloprim,

chlortetracycline, deacetylcefapirin,

doxycycline, erythromycin,
oxytetracycline, tetracycline, and

rafoxanide showed <60% recoveries, but
recovery repeatability values were within
an acceptable limit of <15%RSD. Severe

ion suppression and poor recovery

(<20%) was observed for the dipyrone
hydrate-metabolite, however, no MRL is
established for this veterinary drug in
theegg.

AOAC MRL-based residue screening
in milk and egg

The MRL values of 103 AOAC-listed

targets range from 0.05 to

200μg/kg in milk.3 The method
sensitivity in milk using the 6495C
LC/TQ enabled confident screening of

all targets, except for diclofenac and

norgestomet. Table 1 summarizes the
MRL requirement and observed results

for all targets. Among the comprehensive

target list, 22 have MRL established for
egg under AOAC guidelines, and the
values range from 0.7 to 4,000μg/kg.3

The method sensitivity easily met
the screening requirement for all

MRL-established targets in egg per the
AOAC guidelines.

High blank contribution was observed

for the analysis of chlorhexidine,
clindamycin, progesterone, and

gonadotropin in both milk and egg,

indicating the potential positive
incurrence in the used sample

matrix. Trace residues of ethopabate,
oxibendazole, piperonyl butoxide

ammonia, and tripelennamine

affected the LOQ determination in
milk. Alternatively, the residues from
imidocarb, oxyphenbutazone, piperonyl
butoxide ammonia, and testosterone
affected the LOQ determination in egg.

Method robustness

The method robustness was assessed
by 400 continuous injections of
AgilentVeterinary Drug System Suitability
test mix (part number5799-0015)
postspiked in milk matrix. Peak

responses and RT consistency were

monitored for all 25 targets over time.
The 25 veterinary drug targets are from

10 different chemical classes, with a
broad range of molecular weight, eluted
evenly across the elution window, and
cover both positive and negative polarity
ionization. The dMRM peak area %RSD
and RT %RSD of all 25 targets were

calculated from the 400 injections of

1.0 μg/L postspiked milk blank matrix
extract. The data acquisition was
continuous, and the instrument was
operated without readjusting any tune

parameters. The entire run lasted for

>120 hours.

The elution profile using the InfinityLab
Poroshell column was extremely

consistent over 400 injections. A good

response reproducibility with %RSD
<4.0% and RT %RSD of <0.2% were
observed for all 25 targets. The response
reproducibility of all 25 standards

over 400 injections is summarized in
Figure 5, and an overlay of five total

ion chromatograms (TIC) of Agilent
Veterinary Drug System Suitability test
mix MRM (spread across 400injections)
are shown in Figure 6. The innovative ion
transfer optics design of Agilenttriple

quadrupole mass spectrometers
minimizes the source contamination

from the matrix, thus providing a robust

analytical platform for the confident
analysis of trace veterinary drug residues

(Figure 7). The sample preparation

procedure here provided efficient
sample matrix cleanup, greatly reduced
the matrix residue accumulation on
the ion source interface, and provided
extended column lifetime and detection

consistency. The method robustness,

calculated from a 5-day continuous data

acquisition, confirmed the sustainable
performance of the LC/TQ workflow for

day-to-day operations.

6

500,000

600,000

400

MRM peak area

Injection number

Acquisition time (min)

Counts

400,000

300,000

200,000

100,000

0

050 100 150 200 250 300 350

Figure 5. The response reproducibility of 25 targets included in the Agilent Veterinary Drug System Suitability test mix over 400 continuous injections.
Concentration: postspiked at 1.0 μg/L in milk blank matrix extract (equivalent to a 10 μg/kg matrix spike in milk). Please refer to Table 1 for the list of 25 targets in
the Veterinary Drug System Suitability test mix.

5

×10

2.4

2.2

2.0

1.8

1.6

1.4

1.2

1.0

0.8

0.6

0.4

0.2

0

TIC MRM Injection 400
TIC MRM Injection 300
TIC MRM Injection 200
TIC MRM Injection 100
TIC MRM Injection 001

3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 10.0

Figure 6. Overlay of five selected system suitability mix TIC MRM chromatograms, spread across 400 continuous replicate injections demonstrating the target
elution consistency. Concentration: postextraction spiked at 1.0 μg/L in milk blank matrix extract, LC separation column: Agilent InfinityLab Poroshell 120 EC-C18
(part number 695575-302). (offset X, Y-axis values, injections: 1, 100, 200, 300, and 400).

7

Conclusion

A

B

This study demonstrates the applicability

of the Agilent Comprehensive Veterinary
Drug dMRM Solution for the screening
and quantitation of 210 multiclass

veterinary drug residues in milk and egg
matrices. The workflow-recommended

sample preparation protocol, using

solvent extraction followed by Agilent
Captiva EMR—Lipid cleanup, was shown
to be efficient for target extraction
and matrix cleanup from milk and
egg. The workflow performance was
characterized by good results in terms

of linearity, accuracy, recovery, and

repeatability, allowing sensitive detection

of multiclass veterinary drug residues.

The Agilent 6495C triple quadrupole
LC/MS-based workflow provided
sub-1µg/kg (ppb) LODs for most

analytes, and exceeded the sensitivity

requirements set by global regulatory

Figure 7. The Agilent Jet Stream technology ion source (AJS) of
the Agilent6495C triple quadrupole LC/MS before (A) and after
(B)400continuous injections of milk matrix.

agencies for screening trace veterinary

residues in complex matrices like milk

and eggs. The results demonstrated the

method reliability for routine screening
of over 98% of AOAC-listed veterinary
drug targets from the milk matrix, and
100% of AOAC-listed targets from
the egg matrix. The robustness of
400continuous injections confirmed the
method consistency and reliability, with

minimized sample residue accumulation
on the ion source interface.

8

References

1. Siji Joseph et al. An End-To-End
Workflow for Quantitative Screening

of Multiclass, Multiresidue Veterinary
Drugs in Meat Using the Agilent

6470 Triple Quadrupole LC/MS,

Agilent Technologies application note,

publication number 5994-1932EN.

2. Siji Joseph et al. Quantitative
Screening of Multiresidue Veterinary
Drugs in Seafood Using the Agilent

6470 Triple Quadrupole LC/MS,

Agilent Technologies application note,

publication number 5994-2832EN.

3. Screening and identification method

for regulated veterinary drug residues
in food, AOAC guidelines, Version 7,
June 20, 2018.

4. The United States, Code of Federal

Regulations (CFR) - Title 21,
Tolerance of Residues in New Animal

Drugs in Food, Part 556, volume 6,
April 1, 2019.

5. The United States, Chemical

contaminants of public health
concern used by the Food Safety and
Inspection Service (FSIS), 2017.

6. Pharmacologically active substances

and their classification regarding

maximum residue limits (MRL),

Official Journal of the European
Union, Commission Regulation (EU)

No 37/2010.

7. G5368AA Comprehensive Veterinary

Drug dMRM Solution, Agilent
Technologies workflow guide,

D0002979.

8. Steven J. Lehotay, Utility of the
Summation Chromatographic Peak

Integration Function to Avoid Manual
Reintegrations in the Analysis of
Targeted Analytes, LCGC North

America June 2017, 35(6), 391.

9. Guidelines for Standard Method
Performance Requirements, AOAC
Official Methods of Analysis (2016)

Appendix F.

Appendix

Table 1. Target screening results using milk matrix based on AOAC guidelines. The results were generated based on the Agilent 1290 Infinity II LC and

Agilent 6495C triple quadrupole LC/MS systems. Note that these compounds may be obtained from Agilent, and those highlighted in bold are included
in the Agilent Veterinary Drug System Suitability test mix (part number 5799-0015).

Linear

No. Compound Name

2, 4, 6-triamino-pyrimidine-5-carbonitrile 1.62 Insecticide 465531-97-9 N/A 2.5 5 to 100 105 9

1

2,4-DMA [Amitraz Metabolite] 4.45 Insecticide 33089-74-6 10 0.5 1 to 100 108 3

2

2-Quinoxalinecarboxylic acid [QCA] 4.20 Quinoxalines 879-65-2 N/A 2.5 5 to 100 108 4

3

4-epi-oxytetracycline 4.36 Antibiotic/Tetracycline 14206-58-7 100 0.25 0.5 to 100 84 5

4

4-epi-tetracycline 4.28 Antibiotic/Tetracycline 79-85-6 100 0.25 0.5 to 100 82 4

5

5-Hydroxy thiabendazole 3.59 Anthelmintic/Benzimidazoles 948-71-0 50 0.1 0.25 to 100 110 2

6

5-Hydroxyflunixin 8.36 NSAIDs 75369-61-8 2 0.05 0.1 to 100 112* 10*

7

Acepromazine 7.44 Tranquilizer 61-00-7 N/A 0.05 0.1 to 100 100 2

8

Acetyl isovaleryl tylosin [Tylvalosin] 8.80 Antibiotic/Macrolides 63409-12-1 N/A 0.5 1 to 100 106 5

9

Albendazole 8.09 Anthelmintic/Benzimidazoles 54965-21-8 100 0.05 0.1 to 100 110 2

10

Albendazole sulfone 6.22 Anthelmintic/Benzimidazoles 75184-71-3 100 0.1 0.25 to 100 118 2

11

Albendazole sulfoxide 5.62 Anthelmintic/Benzimidazoles 54029-12-8 100 0.1 0.25 to 100 115 3

12

Albendazole-2-aminosulfone 3.81 Anthelmintic/Benzimidazoles 80983-34-2 100 0.25 0.5 to 100 105 2

13

Alpha Zearalanol 8.33 Hormones 26538-44-3 N/A 1 2.5 to 100 110 4

14

Altrenogest 9.05 Hormones 850-52-2 N/A 0.1 0.25 to 100 112 2

15

Aminoflubendazole 6.19 Anthelmintic/Benzimidazoles 82050-13-3 10 0.05 0.1 to 100 112 2

16

Amoxicillin 2.76 Antibiotic/Beta-lactam 26787-78-0 4 1 2.5 to 100 110* 7*

17

Ampicillin 4.00 Antibiotic/Beta-lactam 69-53-4 4 0.5 1 to 100 115* 7*

18

Amprolium 1.22 Antimicrobial 13082-85-4 N/A 1 2.5 to 100 9 4

19

Azaperone 5.87 Tranquilizer 1649-18-9 N/A 0.1 0.25 to 100 96 3

20

RT

(min)

Functional Use/
Chemical Class CAS Number

AOAC3

MRL

(µg/kg)

(µg/kg)

LOD

Calibration

Curve Range

(μg/kg) with

R2 >0.99

MQC

Recovery

(%)

MQC

RSD

(%)

9

Linear

Calibration

No. Compound Name

Azithromycin 6.27 Antibiotic/Macrolides 83905-01-5 N/A 0.1 0.25 to 100 76 3

21

Baquiloprim 2.74 Antimicrobial 102280-35-3 30 0.25 0.5 to 100 33 4

22

Betamethasone 7.83 Growth promoters/Corticosteroids 378-44-9 0.3 0.25 0.5 to 100 119* 3*

23

Cabergoline 4.72 Dopamine receptor 81409-90-7 0.1 0.05 0.1 to 100 111• 15•

24

Carazolol 6.16 Tranquilizer 57775-29-8 1 0.05 0.1 to 100 119* 5*

25

Carbadox 4.47 Antimicrobial 6804-07-5 N/A 0.25 0.5 to 100 113 3

26

Carprofen 9.08 NSAIDs 53716-49-7 N/A 1 2.5 to 100 106 3

27

Cefalexin 4.00 Antibiotic/Beta-lactam 15686-71-2 100 2.5 5 to 100 87 8

28

Cefalonium 3.98 Antibiotic/Beta-lactam 5575-21-3 20 1 2.5 to 100 73 8

29

Cefapirin 3.28 Antibiotic/Beta-lactam 21593-23-7 60 0.1 0.25 to 100 81 4

30

Cefazolin 4.39 Antibiotic/Beta-lactam 25953-19-9 50 1 2.5 to 100 112 2

31

Cefoperazone 5.21 Antibiotic/Beta-lactam 62893-19-0 50 1 2.5 to 100 114 8

32

Cefquinome 3.75 Antibiotic/Beta-lactam 84957-30-2 20 0.5 1 to 100 63 4

33

Ceftiofur 6.35 Antibiotic/Beta-lactam 80370-57-6 100 0.5 1 to 100 117 3

34

Cefuroxime 4.47 Antibiotic/Beta-lactam 55268-75-2 N/A 2.5 5 to 100 112 6

35

Chloramphenicol 6.34 Antibiotic/Amphenicols 56-75-7 N/A 0.5 1 to 100 113 5

36

Chlorhexidine 7.20 Antimicrobial 55-56-1 N/A 5 10 to 100 95 4

37

Chlormadinone 9.51 Hormones 1961-77-9 2.5 0.5 1 to 100 114* 6*

38

Chlorpromazine 8.16 Tranquilizer 50-53-3 N/A 0.05 0.1 to 100 97 2

39

Chlortetracycline 6.04 Antibiotic/Tetracycline 57-62-5 100 0.25 0.5 to 100 42 9

40

Ciprofloxacin 4.52 Antibiotic/Quinolones 85721-33-1 N/A 0.1 0.25 to 100 95 3

41

Clenbuterol 5.41 Growth promoters/Beta-agonists 37148-27-9 0.05 0.05 0.1 to 100 118• 5•

42

Clindamycin 6.55 Antibiotic/Macrolides 18323-44-9 N/A 10 25 to 100 106# 1#

43

Clopidol 3.61 Coccidiostats 2971-90-6 20 0.25 0.5 to 100 113 2

44

Closantel 10.60 Anthelmintic 57808-65-8 45 0.25 0.5 to 100 93 3

45

Colchicine 6.78 NSAIDs 64-86-8 N/A 0.1 0.25 to 100 105 4

46

Cotinine 2.23 Insecticide 486-56-6 N/A 0.1 0.25 to 100 90 3

47

Coumaphos 9.64 Anthelmintic 56-72-4 N/A 0.5 1 to 100 108 5

48

Cyromazine 2.52 Anthelmintic 66215-27-8 N/A 1 2.5 to 100 94 7

49

Danofloxacin 4.73 Antibiotic/Quinolones 112398-08-0 30 0.05 0.1 to 100 95 2

50

Dapson 4.76 Antibiotic/Sulfonamides 80-08-0 N/A 0.05 0.1 to 100 117 2

51

Dapson N-Acetyl 5.51 Antibiotic/Sulfonamides 565-20-8 N/A 0.25 0.5 to 100 115 3

52

Deacetylcefapirin 2.37 Antibiotic/Beta-lactam 104557-24-6 60 1 2.5 to 100 41 8

53

Diaveridine 3.83 Antimicrobial 5355-16-8 N/A 0.05 0.1 to 100 111 2

54

Diazinon 9.71 Insecticide 333-41-5 20 0.1 0.25 to 100 108 2

55

Diclofenac 9.21 NSAIDs 15307-86-5 0.1 0.25 0.5 to 100 123* 8*

56

Dicloxacillin 8.18 Antibiotic/Beta-lactam 3116-76-5 30 2.5 5 to 100 118 7

57

Dicyclanil 2.98 Insecticide 112636-83-6 N/A 0.25 0.5 to 100 108 1

58

Difloxacin 5.39 Antibiotic/Quinolones 98106-17-3 N/A 0.1 0.25 to 100 106 4

59

Diflubenzuron 9.18 Insecticide 35367-38-5 N/A 0.5 1 to 100 108 5

60

Dimetridazole 3.74 Coccidiostats 551-92-8 N/A 2.5 5 to 100 108 2

61

Diminazene 3.06 Coccidiostats 536-71-0 150 2.5 5 to 100 60 4

62

Dinitolmide [Zoalene] 5.66 Coccidiostats 148-01-6 N/A 0.5 1 to 100 115 3

63

Dipyrone hydrate- metabolite

64

[4-Methylaminoantipyrine]

(min)

3.40 NSAIDs 519-98-2 N/A 0.25 0.5 to 100 54 7

RT

Functional Use/
Chemical Class CAS Number

MRL

(µg/kg)

(µg/kg)

AOAC3

LOD

Curve Range

(μg/kg) with

R2 >0.99

MQC

Recovery

(%)

MQC

RSD

(%)

10

Linear

Calibration

No. Compound Name

Doxycycline 6.36 Antibiotic/Tetracycline 564-25-0 N/A 0.25 0.5 to 100 32 4

65

Emamectin B1a benzoate 10.18 Anthelmintic/Avermectins 121124-29-6 N/A 0.25 0.5 to 100 89 3

66

Emamectin B1b benzoate 9.99 Anthelmintic/Avermectins 121424-52-0 N/A 0.5 1 to 100 95 5

67

Enrofloxacin 4.85 Antibiotic/Quinolones 93106-60-6 100 0.05 0.1 to 100 102 2

68

Erythromycin 7.52 Antibiotic/Macrolides 114-07-8 N/A 0.5 1 to 100 97 13

69

Ethopabate 6.68 Coccidiostats 59-06-3 N/A 0.1 0.25 to 100 115 3

70

Famphur 8.25 Insecticide 52-85-7 N/A 0.25 0.5 to 100 116 7

71

Febantel 9.22 Anthelmintic/Benzimidazoles 58306-30-2 10 0.1 0.25 to 100 115 4

72

Fenbendazole 8.67 Anthelmintic/Benzimidazoles 43210-67-9 10 0.05 0.1 to 100 112 2

73

Fenbendazole Sulfoxide [Oxfendazole] 6.53 Anthelmintic/Benzimidazoles 53716-50-0 10 0.1 0.25 to 100 118 3

74

Firocoxib 8.04 NSAIDs 189954-96-9 N/A 2.5 5 to 100 111 14

75

Florfenicol 5.64 Antibiotic/Amphenicols 73231-34-2 N/A 0.25 0.5 to 100 115 4

76

Fluazuron 10.24 Insecticide 86811-58-7 N/A 0.25 0.5 to 100 108 6

77

Flubendazole 7.80 Anthelmintic/Benzimidazoles 31430-15-6 10 0.05 0.1 to 100 115 2

78

Flugestone acetate 8.42 Hormones 2529-45-5 1 0.5 1 to 100 120* 4*

79

Flumequine 7.47 Antibiotic/Quinolones 42835-25-6 50 0.05 0.1 to 100 119 2

80

Flunixin 8.83 NSAID’s 38677-85-9 N/A 0.05 0.1 to 100 117 2

81

Fluralaner 9.95 Insecticide 864731-61-3 N/A 0.5 1 to 100 111 6

82

Furazolidone 4.77 Antimicrobial/Furans 67-45-8 N/A 1 2.5 to 100 118 5

83

Gamithromycin 6.56 Antibiotic/Aminoglycosides 145435-72-9 N/A 0.1 0.25 to 100 76 4

84

Gonadotropin 7.65 Hormones 33515-09-2 N/A 5 10 to 100 116 7

85

Halofuginone 6.55 Coccidiostats 55837-20-2 N/A 0.25 0.5 to 100 102 5

86

Haloperidol 7.21 Tranquilizer 52-86-8 N/A 0.05 0.1 to 100 116 2

87

Haloxon 8.65 Anthelmintic 321-55-1 N/A 2.5 5 to 100 112 12

88

Imidocarb 3.32 Coccidiostats 27885-92-3 50 1 2.5 to 100 55 5

89

Ipronidazole 6.13 Anthelmintic/Nitroimidazoles 14885-29-1 N/A 1 2.5 to 100 115 3

90

Ipronidazole-OH 4.93 Anthelmintic/Nitroimidazoles 35175-14-5 N/A 0.25 0.5 to 100 114 3

91

Isometamidium 6.09 Anthelmintic 20438-03-3 100 1 2.5 to 100 73 4

92

Josamycin 8.32 Antibiotic/Macrolides 16846-24-5 N/A 0.25 0.5 to 100 110 4

93

Ketamine 4.86 Anesthetic 6740-88-1 N/A 0.05 0.1 to 100 107 2

94

Ketoprofen 8.28 NSAIDs 22071-15-4 50 0.5 1 to 100 118 5

95

Kitasamycin A5 [Leucomycin A5] 7.79 Antibiotic/Aminoglycosides 18361-45-0 N/A 0.25 0.5 to 100 108 4

96

Lasalocid A 11.13 Coccidiostats 25999-31-9 N/A 0.05 0.1 to 100 106 2

97

Leuco Crystal violet 10.44 Fungicides and Dyes 603-48-5 N/A 0.25 0.5 to 100 79 3

98

Leucomalachite green 10.55 Fungicides and Dyes 129-73-7 N/A 0.05 0.1 to 100 96 2

99

Levamisole 3.67 Anthelmintic 14769-73-4 N/A 0.1 0.25 to 100 106 2

100

Lincomycin 3.81 Antibiotic/Aminoglycosides 154-21-2 150 0.05 0.1 to 100 79 3

101

Lufenuron 10.16 Insecticide 103055-07-8 N/A 1 2.5 to 100 113 5

102

Maduramicin Ammonium 11.69 Coccidiostats 79356-08-4 N/A 0.1 0.25 to 100 84 2

103

Malachite green 8.31 Fungicides and Dyes 10309-95-2 N/A 0.05 0.1 to 100 81 2

104

Malathion 9.00 Insecticide 121-75-5 N/A 0.1 0.25 to 100 117 3

105

Marbofloxacin 4.10 Antibiotic/Quinolones 115550-35-1 75 0.1 0.25 to 100 97 2

106

Mebendazole 7.55 Anthelmintic/Benzimidazoles 31431-39-7 N/A 0.05 0.1 to 100 114 2

107

Mefenamic acid 9.75 Anti-inflammatory 61-68-7 N/A 0.1 0.25 to 100 120 3

108

(min)

RT

Functional Use/
Chemical Class CAS Number

MRL

(µg/kg)

(µg/kg)

AOAC3

LOD

Curve Range

(μg/kg) with

R2 >0.99

MQC

Recovery

(%)

MQC

RSD

(%)

11

Linear

Calibration

No. Compound Name

Megestrol acetate 9.49 Hormones 595-33-5 N/A 0.1 0.25 to 100 111 3

109

Melengestrol acetate 9.61 Hormones 2919-66-6 N/A 0.1 0.25 to 100 111 4

110

Meloxicam 8.17 NSAIDs 71125-38-7 15 0.05 0.1 to 100 120 2

111

Methylprednisolone 7.86 Growth promoters/Corticosteroids 83-43-2 2 0.25 0.5 to 100 117* 4*

112

Metoserpate 6.66 Tranquilizer 1178-28-5 N/A 0.1 0.25 to 100 113 3

113

Metronidazole 3.28 Anthelmintic/Nitroimidazoles 443-48-1 N/A 0.1 0.25 to 100 116 2

114

Metronidazole-OH 2.84 Anthelmintic/Nitroimidazoles 4812-40-2 N/A 0.25 0.5 to 100 118 2

115

Monensin 11.30 Coccidiostats 17090-79-8 2 0.1 0.25 to 100 98* 5*

116

Monepantel 9.52 Anthelmintic 851976-50-6 N/A 0.1 0.25 to 100 120 3

117

Morantel tartrate 5.39 Anthelmintic 20574-50-9 50 0.1 0.25 to 100 107 2

118

Moxidectin 11.09 Anthelmintic/Avermectins 113507-06-5 40 0.25 0.5 to 100 115 5

119

Nafcillin 8.10 Antibiotic/Beta-lactam 147-52-4 30 0.25 0.5 to 100 100 4

120

Nalidixic acid 7.29 Antibiotic 389-08-2 N/A 0.05 0.1 to 100 117 2

121

Narasin 11.80 Coccidiostats 55134-13-9 N/A 0.1 0.25 to 100 69 7

122

Neo-Spiramycin 5.75 Antibiotic/Macrolides 70253-62-2 200 0.25 0.5 to 100 70 2

123

Nequinate 9.43 Anthelmintic 13997-19-8 N/A 0.05 0.1 to 100 112 3

124

Netobimin 7.11 Anthelmintic 88255-01-0 100 1 2.5 to 100 108 7

125

Nicarbazine 8.84 Coccidiostats 587-90-6 N/A 0.1 0.25 to 100 116 3

126

Nicotine 1.54 Anti-herbivore 54-11-5 N/A 5 10 to 100 67 8

127

Niflumic Acid 9.14 Anti-inflammatory 4394-00-7 N/A 0.05 0.1 to 100 117 2

128

Nitroxynil 6.77 Anthelmintic 1689-89-0 N/A 0.5 1 to 100 114 5

129

Norfloxacin 4.38 Antibiotic/Quinolones 70458-96-7 N/A 0.1 0.25 to 100 95 2

130

Norgestomet 9.44 Hormones 472-54-8 0.12 1 2.5 to 100 117 3

131

Novobiocin 9.82 Antibiotic 303-81-1 50 0.25 0.5 to 100 120 4

132

Olaquindox 3.03 Growth promoters/Anabolic steroids 23696-28-8 N/A 0.25 0.5 to 100 104 2

133

Oleandomycin 7.13 Antibiotic/Aminoglycosides 3922-90-5 50 0.1 0.25 to 100 112 3

134

Orbifloxacin 5.07 Antibiotic/Quinolones 113617-63-3 20 0.1 0.25 to 100 115 3

135

Ormetoprim 4.49 Antibiotic 6981-18-6 N/A 0.1 0.25 to 100 112 2

136

Oxacillin 7.56 Antibiotic/Beta-lactam 66-79-5 30 1 2.5 to 100 114 9

137

Oxibendazole 6.89 Anthelmintic/Benzimidazoles 20559-55-1 50 0.1 0.25 to 100 116 2

138

Oxolinic acid 6.37 Antibiotic/Quinolones 14698-29-4 N/A 0.25 0.5 to 100 112 2

139

Oxyclozanide 9.56 Anthelmintic 2277-92-1 10 0.25 0.5 to 100 112 4

140

Oxyphenbutazone 8.16 NSAIDs 129-20-4 N/A 0.25 0.5 to 100 105 6

141

Oxytetracycline 4.54 Antibiotic/Tetracycline 79-57-2 100 0.25 0.5 to 100 46 9

142

Penicillin G 7.00 Antibiotic/Beta-lactam 61-33-6 N/A 0.5 1 to 100 90 7

143

Penicillin V [Phenoxymethylpenicillin] 7.41 Antibiotic/Beta-lactam 87-08-1 N/A 1 2.5 to 100 117 2

144

Phenylbutazone 9.09 NSAIDs 50-33-9 N/A 0.25 0.5 to 100 106 3

145

Phosalone 9.77 Insecticide 2310-17-0 N/A 0.5 1 to 100 113 9

146

Phoxim 9.70 Insecticide 14816-18-3 N/A 1 2.5 to 100 110 10

147

Piperonyl butoxide Ammonia 10.31 Insecticide 51-03-6 50 0.1 0.25 to 100 116 1

148

Pirlimycin 6.10 Antibiotic/Aminoglycosides 79548-73-5 100 1 2.5 to 100 96 4

149

Praziquantel 8.57 Anthelmintic 55268-74-1 N/A 0.25 0.5 to 100 116 2

150

Prednisolone 7.29 Growth promoters/Corticosteroids 50-24-8 6 0.1 0.25 to 100 120* 2*

151

Prednisone 7.13 Growth promoters/Corticosteroids 53-03-2 N/A 0.25 0.5 to 100 117 3

152

(min)

RT

Functional Use/
Chemical Class CAS Number

MRL

(µg/kg)

(µg/kg)

AOAC3

LOD

Curve Range

(μg/kg) with

R2 >0.99

MQC

Recovery

(%)

MQC

RSD

(%)

12

Linear

Calibration

No. Compound Name

Progesterone 9.60 Hormones 57-83-0 N/A 10 25 to 100 117# 4#

153

Propionylpromazin 8.00 Antiemetic 3568-24-9 N/A 0.05 0.1 to 100 98 2

154

Propyphenazone 7.68 NSAIDs 479-92-5 N/A 0.05 0.1 to 100 118 3

155

Pyrantel 4.29 Anthelmintic 15686-83-6 N/A 0.1 0.25 to 100 105 2

156

Pyrimethamine 6.31 Antimicrobial 58-14-0 N/A 0.05 0.1 to 100 112 2

157

Ractopamine 4.66 Growth promoters/Beta-agonists 97825-25-7 N/A 0.1 0.25 to 100 117 1

158

Rafoxanide 11.11 Anthelmintic 22662-39-1 10 0.25 0.5 to 100 70 4

159

Rifaximin 9.07 Antibiotic 80621-81-4 60 0.1 0.25 to 100 104 4

160

Robenidine 8.58 Coccidiostats 25875-51-8 N/A 0.25 0.5 to 100 101 4

161

Ronidazole 3.40 Anthelmintic/Nitroimidazoles 7681-76-7 N/A 0.1 0.25 to 100 119 2

162

Salbutamol [Albuterol] 3.03 Growth promoters/Beta-agonists 18559-94-9 N/A 0.05 0.1 to 100 105 2

163

Salinomycin 11.62 Coccidiostats 53003-10-4 N/A 0.1 0.25 to 100 77 6

164

Sarafloxacin 5.39 Antibiotic/Quinolones 98105-99-8 N/A 0.1 0.25 to 100 107 3

165

Spiramycin I 6.13 Antibiotic/Macrolides 24916-50-5 200 0.25 0.5 to 100 82 2

166

Sulfabenzamide 6.07 Antibiotic/Sulfonamides 127-71-9 100 0.05 0.1 to 100 120 2

167

Sulfacetamide 3.13 Antibiotic/Sulfonamides 144-80-9 100 0.1 0.25 to 100 115 2

168

Sulfachloropyridazine 5.25 Antibiotic/Sulfonamides 80-32-0 100 0.1 0.25 to 100 116 2

169

Sulfaclozine 6.30 Antibiotic/Sulfonamides 102-65-8 100 0.25 0.5 to 100 116 4

170

Sulfadiazine [Silvadene] 3.42 Antibiotic/Sulfonamides 68-35-9 100 0.1 0.25 to 100 120 2

171

Sulfadimethoxine 6.44 Antibiotic/Sulfonamides 122-11-2 100 0.05 0.1 to 100 119 2

172

Sulfadimidine [Sulfamethazine] 4.62 Antibiotic/Sulfonamides 57-68-1 100 0.1 0.25 to 100 114 2

173

Sulfadoxine 5.58 Antibiotic/Sulfonamides 2447-57-6 100 0.05 0.1 to 100 119 2

174

Sulfaethoxypyridazine 5.93 Antibiotic/Sulfonamides 963-14-4 100 0.05 0.1 to 100 117 1

175

Sulfaguanidine 1.72 Antibiotic/Sulfonamides 57-67-0 100 0.25 0.5 to 100 107 1

176

Sulfamerazine 4.02 Antibiotic/Sulfonamides 127-79-7 100 0.1 0.25 to 100 118 1

177

Sulfameter [sulfamethoxydiazine] 4.48 Antibiotic/Sulfonamides 651-06-9 100 0.1 0.25 to 100 113 2

178

Sulfamethizole 4.50 Antibiotic/Sulfonamides 144-82-1 100 0.1 0.25 to 100 115 3

179

Sulfamethoxazole 5.47 Antibiotic/Sulfonamides 723-46-6 100 0.1 0.25 to 100 116 2

180

Sulfamethoxypyridazine 4.68 Antibiotic/Sulfonamides 80-35-3 100 0.1 0.25 to 100 114 1

181

Sulfamonomethoxine 5.23 Antibiotic/Sulfonamides 1220-83-3 100 0.1 0.25 to 100 118 2

182

Sulfamoxole 4.31 Antibiotic/Sulfonamides 729-99-7 100 0.05 0.1 to 100 112 2

183

Sulfanitran 7.33 Antibiotic/Sulfonamides 122-16-7 100 1 2.5 to 100 115 7

184

Sulfaphenazole 6.34 Antibiotic/Sulfonamides 526-08-9 100 0.1 0.25 to 100 115 3

185

Sulfapyridine 3.83 Antibiotic/Sulfonamides 144-83-2 100 0.1 0.25 to 100 115 1

186

Sulfaquinoxaline 6.51 Antibiotic/Sulfonamides 59-40-5 100 0.1 0.25 to 100 119 3

187

Sulfathiazole 3.62 Antibiotic/Sulfonamides 72-14-0 100 0.1 0.25 to 100 115 2

188

Sulfisomidine 3.34 Antibiotic/Sulfonamides 515-64-0 100 0.05 0.1 to 100 111 1

189

Sulfisoxazole 5.76 Antibiotic/Sulfonamides 127-69-5 100 0.1 0.25 to 100 113 3

190

Sulindac 8.03 Antibiotic/Sulfonamides 38194-50-2 100 0.1 0.25 to 100 117 3

191

Teflubenzuron 10.08 Insecticide 83121-18-0 N/A 0.5 1 to 100 117 3

192

Testosterone 8.56 Growth promoters/Anabolic steroids 58-22-0 N/A 0.1 0.25 to 100 119 4

193

Tetracycline 4.78 Antibiotic/Tetracycline 60-54-8 100 0.25 0.5 to 100 64 4

194

Thiabendazole 4.34 Anthelmintic/Benzimidazoles 148-79-8 50 0.05 0.1 to 100 112 2

195

Thiamphenicol 4.33 Antibiotic/Amphenicols 15318-45-3 50 0.25 0.5 to 100 120 3

196

(min)

RT

Functional Use/
Chemical Class CAS Number

MRL

(µg/kg)

(µg/kg)

AOAC3

LOD

Curve Range

(μg/kg) with

R2 >0.99

MQC

Recovery

(%)

MQC

RSD

(%)

13

Linear

Calibration

No. Compound Name

Tiamulin 7.68 Antibiotic 55297-95-5 N/A 0.05 0.1 to 100 114 2

197

Tilmicosin 6.87 Antibiotic/Macrolides 108050-54-0 50 1 2.5 to 100 90 7

198

Tolfenamic acid 9.94 NSAIDs 13710-19-5 50 1 2.5 to 100 114 8

199

Trenbolone 7.98 Growth promoters/Anabolic steroids 10161-33-8 N/A 0.25 0.5 to 100 111 4

200

Trichlorfon [DEP] 5.29 Tranquilizer 52-68-6 50 0.5 1 to 100 124 3

201

Triclabendazole 9.74 Anthelmintic/Benzimidazoles 68786-66-3 10 0.1 0.25 to 100 113 2

202

Trimethoprim 4.12 Antibiotic 738-70-5 50 0.1 0.25 to 100 108 3

203

Tripelennamine 6.39 Anthelmintic 91-81-6 20 0.05 0.1 to 100 105 2

204

Tylosin 7.64 Antibiotic/Macrolides 1401-69-0 50 0.5 1 to 100 109 5

205

Valnemulin 8.39 Antibiotic 101312-92-9 N/A 0.1 0.25 to 100 113 3

206

Vedaprofen 9.14 NSAIDs 71109-09-6 N/A 0.1 0.25 to 100 114 2

207

Virginiamycin M1 8.21 Antibiotic/Macrolides 21411-53-0 N/A 0.1 0.25 to 100 114 5

208

Xylazine 5.24 Tranquilizer 7361-61-7 N/A 0.1 0.25 to 100 109 2

209

Zilpaterol 2.98 Growth promoters/Beta-agonists 119520-05-7 N/A 0.1 0.25 to 100 96 3

210

(min)

RT

Functional Use/
Chemical Class CAS Number

MRL

(µg/kg)

(µg/kg)

AOAC3

LOD

Curve Range

(μg/kg) with

R2 >0.99

• Data using LLQC, * Data using LQC, # Data using HQC

MQC

Recovery

(%)

MQC

RSD

(%)

www.agilent.com/chem

DE44270.4828125

This information is subject to change without notice.

© Agilent Technologies, Inc. 2021
Printed in the USA, March 15, 2021
5994-3124EN