Agilent High Sensitivity RNA ScreenTape Assay Quick Start Guide
High Sensitivity RNA ScreenTape Assay for TapeStation
Systems
Quick Guide
The Agilent 4150 (G2992AA) and 4200 (G2991AA and G2991BA) TapeStation systems are automated platforms for
scalable, flexible, fast, and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 4150 and 4200 TapeStation systems only. A Quick Guide specific
for use with the Agilent 2200 TapeStation system is available online.
The High Sensitivity RNA ScreenTape assay is designed for analyzing and assessing integrity of eukaryotic and
prokaryotic total RNA.
Specifications
Analytical specifications High Sensitivity RNA ScreenTape assay
Sensitivity
Quantitative precision 15 % CV
Quantitative accuracy ±30 %
Quantitative range 500 – 10000 pg/µL
RIN
Maximum buffer concentration in
sample
Physical specifications
Analysis time 16 samples: <35 min, 96 samples: <180 min
Samples per consumable 16
Sample volume required 2 µL
Kit stability 4 months
Kit size 112 samples
1
2
1
e
functional range
Signal-to-noise >3 (single peak)
RINe – RNA integrity number equivalent
2
100 pg/µL
1000 – 25000 pg/µL
10 mM Tris, 1 mM EDTA
Agilent High Sensitivity RNA ScreenTape Quick Guide for TapeStation Systems
High Sensitivity RNA ScreenTape Assay for TapeStation Systems
Storage Conditions
• Store High Sensitivity RNA sample buffer and ScreenTape devices: 2 – 8 °C (36 – 46 °F).
• Store High Sensitivity RNA ladder at -20 °C to -5 °C (-4 °F to 23 °F).
• Store partially used ScreenTape devices upright at 2 – 8 °C (36 – 46 °F) for a maximum of 2 weeks.
• Never freeze ScreenTape devices. Discard any accidentally frozen ScreenTape devices.
Kit Components
Part Number Name Color Amount
5067-5579 High Sensitivity RNA ScreenTape 7 ScreenTape devices
5067-5580 High Sensitivity RNA ScreenTape Sample Buffer 1 vial, 250 µL
5067-5581 High Sensitivity RNA ScreenTape Ladder 1 vial, 10 µL
Limited Use Label License
Some products within this assay contain SYBR™ Gold, which is licensed from Life Technologies Corporation for use in
research and development only. SYBR™ is a registered trademark of Life Technologies Corporation.
For Research Use Only
Not for use in Diagnostic Procedures.
Additional Material Required for Analysis with the TapeStation Systems
• Loading tips (5067-5598, 1 pk or 5067-5599, 10 pk)
• Optical Tube 8x Strip (401428) and Optical Tube Cap 8x Strip (401425)
• Vortex mixer IKA MS3 with 96-well sample plate adapter
• 96-well sample plates (5042-8502) and 96-well Plate Foil Seal (5067-5154) (4200 TapeStation systems only)
Additional Equipment Required (Not Supplied)
• Volumetric micropipettes for handling volumes from 1 to 15 µL
• Centrifuges for tube strips and 96-well sample plates
• Heating block or PCR cycler compatible with 200 µL tube strip vials and full skirted 96-well sample plates
WARN I NG
CA UTI ON
2
Toxic agents
Refer to product material safety datasheets for further information.
When working with the ScreenTape assay follow the appropriate safety procedures such as
wearing safety goggles, laboratory gloves and protective clothing.
Damage to the TapeStation systems
Only use the recommended consumables and reagents with the TapeStation systems.
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