Agilent Genomic DNA ScreenTape Assay Quick Start Guide
Genomic DNA ScreenTape Assay for TapeStation Systems
Quick Guide
The Agilent 4150 (G2992AA) and 4200 (G2991AA and G2991BA) TapeStation systems are automated platforms for
scalable, flexible, fast, and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 4150 and 4200 TapeStation systems only. A Quick Guide specific
for use with the Agilent 2200 TapeStation system is available online.
The Genomic DNA ScreenTape assay is designed for assessing integrity of genomic DNA samples and analyzing
double-stranded DNA molecules from 200 to > 60000 basepairs.
Specifications
Analytical specifications Genomic DNA ScreenTape assay
Sizing range 200 to >60000 bp
Sensitivity
Sizing precision
Sizing accuracy
Quantitative precision 15 % CV
Quantitative accuracy ±20 %
Quantitative range 10 – 100 ng/µL
DIN functional range
Maximum buffer concentration in
sample
Physical specifications
Analysis time 15 samples: <25 min, 96 samples: <140 min
Samples per consumable 15
Sample volume required 1 µL
Kit stability 4 months
Kit size 105 samples
1
2
3
1
2
2
3
Signal-to-noise >3 (single peak)
Determined using the Genomic DNA Ladder as sample
DIN - DNA Integrity Number
0.5 ng/µL
200 – 15000 bp: 15 % CV
200 – 15000 bp: ±15 %
5 – 300 ng/µL
10 mM MgCl
1 µg/µL glycogen
, 50 mM NaCl, 10 mM NaOAc, 10 % ethanol, 10 % 2-propanol,
2
Agilent Genomic DNA ScreenTape Quick Guide for TapeStation Systems
Genomic DNA ScreenTape Assay for TapeStation Systems
Storage Conditions
• Reagent vials and ScreenTape devices: 2 – 8 °C (36 – 46 °F).
• Store partially used ScreenTape devices upright at 2 – 8 °C (36 – 46 °F) for a maximum of 2 weeks.
• Never freeze ScreenTape devices. Discard any accidentally frozen ScreenTape devices.
Kit Components
Part Number Name Color Amount
5067-5365 Genomic DNA ScreenTape 7 ScreenTape devices
5067-5366 Genomic DNA Reagents
• Genomic DNA Ladder
• Genomic DNA Sample Buffer
2 vials
25 µL
1350 µL
Limited Use Label License
Some products within this assay contain SYBR™ Green I, which is licensed from Life Technologies Corporation for use
in research and development only. SYBR™ is a registered trademark of Life Technologies Corporation.
For Research Use Only
Not for use in Diagnostic Procedures.
Additional Material Required for Analysis with the TapeStation Systems
• Loading tips (5067-5598, 1 pk or 5067-5599, 10 pk)
• Optical Tube 8x Strip (401428) and Optical Tube Cap 8x Strip (401425)
• Vortex mixer IKA MS3 with 96-well sample plate adapter
• 96-well sample plates (5042-8502) and 96-well Plate Foil Seal (5067-5154) (4200 TapeStation systems only)
Additional Equipment Required (Not Supplied)
• Volumetric micropipettes for handling volumes from 1 to 15 µL
• Centrifuges for tube strips and 96-well sample plates
WARN IN G
CAU T IO N
2
Toxic agents
Refer to product material safety datasheets for further information.
When working with the ScreenTape assay follow the appropriate safety procedures such as
wearing safety goggles, laboratory gloves and protective clothing.
Damage to the TapeStation systems
Only use the recommended consumables and reagents with the TapeStation systems.
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