Agilent DNF-935 Quick Start Guide
Analytical specifications
dsDNA 935 assay
DNA Sizing Range
100 bp – 1,500 bp
DNA Sizing Accuracy1
+ 15% or better
DNA Fragment Concentration Range1
DNA Sizing Precision1
2% CV
Physical Specifications
Total electrophoresis run time
22cm2: 15 minutes, 33cm: 20 minutes, 55cm: 35 minutes
Samples per run
12, 48 or 96; depending on the instrument type
Sample volume required
2 µL (adjustable depending on sample concentration)
Kit stability
4 months
Agilent DNF-935 Reagent Kit
(1 – 1500 bp) Quick Guide for
Fragment Analyzer Systems
The Agilent Fragment Analyzer systems are automated capillary electrophoresis platforms for scalable, flexible, fast,
and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-935
Reagent kit from Agilent is for the fast analysis of dsDNA fragments between 100 bp and 1,500 bp. Sizing and relative
quantification between samples can be obtained using this kit. Example applications include general PCR fragment sizing and
QC, and genotyping.
Specifications
0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution sample)
Separation Resolution
1
Results using DNA Ladder of DNA Fragment standards initially prepared in 1x TE buffer.
2
The 22 cm effective, 47 cm total length capillary is only available for 12-capillary Fragment Analyzer instruments
1 bp – 100 bp ≤ 15%; 100 bp – 1,500 bp ≤ 10% (22-47 Array)
1 bp – 100 bp ≤ 15%; 100 bp – 1,500 bp ≤ 10% (33-55 Array)
1 bp – 1,500 bp ≤ 5% (55-80 Array)
1
DNF-935 dsDNA Reagent Kit (1-1500 bp) Quick Guide for Fragment Analyzer
1
WARNING
•
•
Kit Components – 500 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6599*
DNF-930-0240 dsDNA 930 Gel, 240 mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125 mL
DNF-495-0060 Dilution Buffer 1X TE, 60mL
DNF-935-FR*
DNF-600-U030
FS-SLR910-0001
FA-MRK910F-0003
5191-6614* Qualitative DNA, 500, RT
FS-SMO15 Mineral Oil Dropper Bottle, 15mL 1
DNF-475-0050 5x Capillary Conditioning Soln, 50 mL 1
dsDNA 930/935, 500, 4oC
• Dilute with sub-micron filtered water prior
to use
dsDNA 935, FR
Intercalating Dye, 30 μL
100bp DNA Ladder, 100 µL
Markers, 1bp & 1500kb, 3.2 mL
1
1
1
1
*not orderable
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
2
DNF-935 dsDNA Reagent Kit (1-1500 bp) Quick Guide for Fragment Analyzer
1
WARNING
•
•
Kit Components – 1000 Sample Kit
Kit Component
Number
5191-6600*
DNF-930-FR*
5191-6615* Qualitative DNA, 1000/5000, RT
FS-SMO15 Mineral Oil Dropper Bottle, 15mL 1
DNF-475-0100 5x Capillary Conditioning Soln, 100 mL 1
Part Number
(Re-order Number)
DNF-930-0500 dsDNA 930 Gel, 500 mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300 mL
DNF-495-0125 Dilution Buffer 1X TE, 125 mL
DNF-600-U030
FS-SLR910-0001
FA-MRK910F-0003
Description Quantity Per Kit
dsDNA 930/935, 1000, 4oC
• Dilute with sub-micron filtered water prior
to use
dsDNA 930, FR
Intercalating Dye, 30 μL
100bp DNA Ladder, 100 µL
Markers, 1bp & 1500kb, 3.2 mL
1
2
2
2
*not orderable
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
3
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