Agilent DNF-915 Quick Start Guide
Analytical specifications
dsDNA 915 assay
DNA Sizing Range
35 bp – 5,000 bp
DNA Sizing Accuracy1
+ 5% or better
DNA Fragment Concentration Range1
DNA Sizing Precision1
2% CV
Physical Specifications
Total electrophoresis run time
22 cm2: 22 minutes, 33 cm: 45 minutes, 55 cm: 70 minutes
Samples per run
12, 48 or 96; depending on the instrument type
Sample volume required
2 µL (adjustable depending on sample concentration)
Kit stability
4 months
Agilent DNF-915 Reagent Kit
(35 – 5000 bp) Quick Guide for
Fragment Analyzer Systems
The Agilent Fragment Analyzer systems are automated capillary electrophoresis platforms for scalable, flexible, fast,
and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The dsDNA 915
Reagent kit from Agilent is for the analysis of dsDNA fragments between 35 and 5,000 bp. Sizing and relative quantification
between samples can be obtained using this kit. Example applications include general PCR fragment sizing and QC, and
genotyping.
Specifications
0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution sample)
Separation Resolution
1
Results using DNA Ladder of DNA Fragment standards initially prepared in 1x TE buffer.
2
The 22 cm effective, 47 cm total length capillary is only available for 12-capillary Fragment Analyzer instruments
35 –100 bp ≤ 10%; 100 –700 bp ≤ 5%; 700 -5,000 bp ≤ 10% (22-47 Array)
35 –100 bp ≤ 10%; 100 – 1,000 bp ≤ 5%; 1,000 – 5,000 bp ≤10% (33-55 Array)
35 –1,200 bp ≤ 5%; 1,200 –5,000 bp ≤ 6% (55-80 Array)
1
DNF-915 dsDNA Reagent Kit (35-5000 bp) Quick Guide for Fragment Analyzer
1
WARNING
•
•
Kit Components – 500 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6595*
DNF-810-0240 dsDNA 810 Gel, 240 mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125 mL
DNF-495-0060 Dilution Buffer 1X TE, 60mL
DNF-915-FR*
DNF-600-U030
FS-SLR915-0001
FS-SMK480-0003
5191-6614* Qualitative DNA, 500, RT
FS-SMO15 Mineral Oil Dropper Bottle, 15mL 1
DNF-475-0050 5x Capillary Conditioning Soln, 50 mL 1
dsDNA 910/915, 500, 4oC
• Dilute with sub-micron filtered water prior
to use
dsDNA 915 (35-5000), FR
Intercalating Dye, 30 μL
100bp DNA Plus Ladder, 1mL
Markers, 35bp & 5000bp, 3.2 mL
1
1
1
1
*not orderable
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
2
DNF-915 dsDNA Reagent Kit (35-5000 bp) Quick Guide for Fragment Analyzer
1
WARNING
•
•
Kit Components – 1000 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6596*
DNF-810-0500 dsDNA 910 Gel, 500 mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300 mL
DNF-495-0125 Dilution Buffer 1X TE, 125mL
DNF-915-FR*
DNF-600-U030
FS-SLR915-0001
FS-SMK480-0003
5191-6615* Qualitative DNA, 1000-5000, RT
FS-SMO15 Mineral Oil Dropper Bottle, 15mL 1
DNF-475-0100 5x Capillary Conditioning Soln, 100 mL 1
*not orderable
dsDNA 910/915, 1000, 4oC
• Dilute with sub-micron filtered water prior
to use
dsDNA 915 (35bp-5000bp), FR
Intercalating Dye, 30 μL
100bp DNA Plus Ladder, 1mL
Markers, 35bp & 5000bp, 3.2 mL
1
2
2
2
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
3
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