Agilent DNF-905 Quick Start Guide
Analytical specifications
dsDNA 905 assay
DNA Sizing Range
35 bp - 500 bp
DNA Sizing Accuracy1
+ 5% or better
DNA Fragment Concentration Range1
DNA Sizing Precision1
2% CV
Physical Specifications
Total electrophoresis run time
22cm2: 28 minutes, 33cm: 60 minutes, 55cm: 80 minutes
Samples per run
12, 48 or 96; depending on the instrument type
Sample volume required
2 µL (adjustable depending on sample concentration)
Kit stability
4 months
Agilent DNF-905 dsDNA Kit
(1-500 bp) Quick Guide for
Fragment Analyzer Systems
The Agilent Fragment Analyzer systems are automated capillary electrophoresis platforms for scalable, flexible, fast,
and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only. The DNF-905
reagent kit from Agilent is for the analysis of dsDNA fragments between 35 bp and 500 bp. Sizing and relative quantification
between samples can be obtained using this kit. Example applications include general PCR fragment sizing and QC,
microsatellite/SSR analysis, and genotyping.
Specifications
0.5 ng/µL – 50 ng/µL input DNA (adjustable by dilution sample)
Separation Resolution
1
Results using DNA Ladder of DNA Fragment standards initially prepared in 1x TE buffer.
2
The 22 cm effective, 47 cm total length capillary is only available for 12-capillary Fragment Analyzer instruments
8 – 10 bp @ 300 bp (22-47 array)
5 bp @ 300 bp (33-55 array)
3 – 5 bp @ 300 bp (55-80 array)
1
DNF-905 dsDNA Reagent Kit (1-500 bp) Quick Guide for Fragment Analyzer
1
WARNING
•
•
Kit Components – 500 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6593*
DNF-905-0240 dsDNA 905 Gel, 240 mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125 mL
DNF-495-0060 Dilution Buffer 1X TE, 60mL
DNF-905-FR*
DNF-600-U030
FS-SLR905-0001
FS-MRK900F-0003
5191-6614* Qualitative DNA, 500, RT
FS-SMO15 Mineral Oil Dropper Bottle, 15mL 1
DNF-475-0050 5x Capillary Conditioning Soln, 50 mL 1
dsDNA 905 (1-500bp), 500, 4oC
• Dilute with sub-micron filtered water prior
to use
dsDNA 905 (1-500 bp), FR
Intercalating Dye, 30 μL
35-400bp DNA Ladder, 1mL
Markers, 1bp & 500bp, 3.2 mL
*not orderable
1
1
1
1
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
2
DNF-905 dsDNA Reagent Kit (1-500 bp) Quick Guide for Fragment Analyzer
1
WARNING
•
•
Kit Components – 1000 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6594*
DNF-905-0500 dsDNA 905 Gel, 500 mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300 mL
DNF-495-0125 Dilution Buffer 1X TE, 125mL
DNF-905-FR*
DNF-600-U030
FS-SLR905-0001
FS-MRK900F-0003
5191-6614* Qualitative DNA, 1000/5000
FS-SMO15 Mineral Oil Dropper Bottle, 15mL 1
DNF-475-0100 5x Capillary Conditioning Soln, 100 mL 1
*not orderable
dsDNA 905 (1-500bp), 1000, 4oC
• Dilute with sub-micron filtered water prior
to use
dsDNA 905 (1-500 bp), FR
Intercalating Dye, 30 μL
35-400bp DNA Ladder, 1mL
Markers, 1bp & 500bp, 3.2 mL
, RT
1
2
2
2
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
3
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