Agilent DNF-488 Quick Start Guide
Analytical specifications
HS Genomic DNA assay
Sizing Range
50 bp – 20,000 bp
gDNA Concentration Range2
50 pg/µL – 5 ng/µL input gDNA
gDNA Quantification Precision2
gDNA Quantification Accuracy2
+ 25%
Physical Specifications3
Total electrophoresis run time
22cm1: 30 minutes, 33cm: 50 minutes, 55cm: 80 minutes
Samples per run
12, 48 or 96; depending on the instrument type
Sample volume required
2 µL
Kit stability
4 months
Agilent DNF-488 HS Genomic DNA
Kit Quick Guide for Fragment
Analyzer Systems
The Agilent Fragment Analyzer systems are automated capillary electrophoresis platforms for scalable,
flexible, fast, and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only.
Specifications
15% CV
Maximum gDNA Concentration
1
The FA 12-Capillary Array Ultrashort, 22 cm is only available for 5200 Fragment Analyzer system.
2
Results using human genomic DNA as standards initially prepared in 1x TE buffer.
5 ng/µL
1
DNF-488 HS Genomic DNA Quick Guide for Fragment Analyzer Systems
•
•
Kit Components – 500 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6585*
DNF-270-0240 HS Genomic DNA Separation Gel, 240 mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125 mL
DNF-497-0125 0.25x TE Rinse Buffer, 125 mL 1
DNF-488-FR*
DNF-600-U030
DNF-375-0003
DNF-377-U100 HS Genomic DNA Ladder, 100 μL 1
DNF-475-0050 DNF-475-0050 5x Capillary Conditioning Soln, RT
*Not orderable.
HS Genomic DNA 500, 4oC
• Dilute with sub-micron filtered water prior
to use
HS Genomic DNA, FR
Intercalating Dye, 30 μL
HS Genomic DNA Diluent Marker, 2.4 mL
• Dilute with sub-micron filtered water prior
to use
1
1
5
1
WARNING
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
2
DNF-488 HS Genomic DNA Quick Guide for Fragment Analyzer Systems
•
•
Kit Components – 1000 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6586*
DNF-270-0500 HS Genomic DNA Separation Gel, 500 mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300 mL
DNF-497-0125 0.25x TE Rinse Buffer, 125 mL 1
DNF-488-FR*
DNF-600-U030
DNF-375-0003
DNF-377-U100 HS Genomic DNA Ladder, 100 μL 2
DNF-475-0100 DNF-475-0100 5x Capillary Conditioning Soln, RT
*Not orderable.
HS Genomic DNA, 1000, 4oC
• Dilute with sub-micron filtered water prior
to use
HS Genomic DNA, FR
Intercalating Dye, 30 μL
HS Genomic DNA Diluent Marker, 2.4 mL
• Dilute with sub-micron filtered water prior
to use
1
2
10
1
WARNING
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
3
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