Agilent DNF-474 HS NGS Quick Start Guide
Analytical specifications
HS NGS Fragment assay
Sizing Range
100 bp – 6,000 bp
Sizing Accuracy2
+ 5% or better
Sizing Precision2
2% CV
Fragment Concentration Range2
5 pg/µL – 500 pg/µL input DNA
Smear Concentration Range
50 pg/µL – 5,000 pg/µL input DNA
Quantification Accuracy
+ 25%
Quantification Precision
15% CV
Quantitative Range
Fragment3:5 - 500 pg/µL; Smear3:50 – 5,000 pg/µL
Physical Specifications
Total electrophoresis run time
22cm1: 25 minutes, 33cm: 50 minutes, 55cm: 80 minutes
Samples per run
12, 48 or 96; depending on the instrument type
Sample volume required
2 µL
Kit stability
4 months
Agilent DNF-474 HS NGS
Fragment Kit Quick Guide for
Fragment Analyzer Systems
The Agilent Fragment Analyzer systems are automated capillary electrophoresis platforms for scalable,
flexible, fast, and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 5200, 5300, and 5400 Fragment Analyzer systems only.
The DNF-474 HS NGS Fragment assay is designed for the quantitative and qualitative analysis of NGS libraries
and their intermediates from 100 to 6,000 bp.
Specifications
1,2
Separation Resolution
2,3
2,3
1
The FA 12-Capillary Array Ultrashort, 22 cm is only available for 5200 Fragment Analyzer system.
2
Results using DNA ladder in 1X TE buffer.
3
Results using DNA samples in 1X TE buffer.
100 bp – 2,000 bp < 5%
2,000 bp – 5,000 bp < 10%
1
DNF-474 HS NGS Fragment Quick Guide for Fragment Analyzer Systems
•
•
Kit Components – 500 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-6578*
DNF-240-0240 NGS Separation Gel, 240 mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0125 5x 930 dsDNA Inlet Buffer, 125 mL
DNF-497-0125 0.25x TE Rinse Buffer, 125 mL 1
DNF-474-FR*
DNF-600-U030
DNF-373-0003
DNF-396-U100 HS NGS DNA Ladder, 100 μL 1
DNF-475-0050 DNF-475-0050 5x Capillary Conditioning Soln, RT
*Not orderable.
HS NGS Fragment (1-6000bp), 500, 4oC
• Dilute with sub-micron filtered water prior
to use
HS NGS Frag (1-6000bp), -20oC
Intercalating Dye, 30 μL
HS NGS Diluent Marker (1-6000bp), 2.4 mL
• Dilute with sub-micron filtered water prior
to use
1
1
5
1
WARNING
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
2
DNF-474 HS NGS Fragment Quick Guide for Fragment Analyzer Systems
•
•
Kit Components – 1000 Sample Kit
Kit Component
Number
Part Number
(Re-order Number)
Description Quantity Per Kit
5191-5679*
DNF-240-0500 NGS Separation Gel, 500 mL 1
DNF-300-0008 BF-25 Blank Solution, 8mL 1
DNF-355-0300 5x 930 dsDNA Inlet Buffer, 300 mL
DNF-497-0125 0.25x TE Rinse Buffer, 125 mL 1
DNF-476-FR*
DNF-600-U030
DNF-362-0003
DNF-363-U100 HS NGS DNA Ladder, 100 μL 2
DNF-475-0100 DNF-475-0100 5x Capillary Conditioning Soln, RT
*Not orderable.
HS NGS Fragment (1-6000bp), 1000, 4oC
• Dilute with sub-micron filtered water prior
to use
HS NGS Frag (1-6000bp), -20oC
Intercalating Dye, 30 μL
HS NGS Diluent Marker (1-6000bp), 2.4 mL
• Lower marker (set to 1 bp) and 6,000 bp
upper marker
• Fragments from 100 bp – 3,000 bp;
1 ng/µL total DNA concentration
• Dilute with sub-micron filtered water prior
to use
1
2
10
1
WARNING
Refer to product safety data sheets for further information
When working with the Fragment Analyzer kit components follow the appropriate safety
procedures such as wearing goggles, safety gloves and protective clothing.
3
Loading...