Agilent D5000 Quick Start Guide
D5000 ScreenTape Assay for TapeStation Systems
Quick Guide
The Agilent 4150 (G2992AA) and 4200 (G2991AA and G2991BA) TapeStation systems are automated platforms for
scalable, flexible, fast, and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 4150 and 4200 TapeStation systems only. A Quick Guide specific
for use with the Agilent 2200 TapeStation system is available online.
The D5000 ScreenTape assay is designed for analyzing double-stranded DNA molecules from 100 to 5000 basepairs.
Specifications
Analytical specifications D5000 ScreenTape assay
Sizing range 100 – 5000 bp
Typical resolution 400 – 5000 bp: 15 %
Sensitivity
Sizing precision
Sizing accuracy
Quantitative precision 0.1 – 1 ng/µL: 15 % CV, 1 – 50 ng/µL: 10 % CV
Quantitative accuracy ±20 %
Quantitative range 0.1 – 50 ng/µL
Maximum buffer concentration in
sample
Physical specifications
Analysis time 15 samples: <25 min, 96 samples: <135 min
Samples per consumable 15
Sample volume required 1 µL
Kit stability 4 months
Kit size 105 samples
1
2
1
2
2
Signal-to-noise >3 (single peak)
Determined using D5000 Ladder as sample
0.1 ng/µL
5 % CV
±10 %
250 mM KCl, 250 mM Tris-HCl, 125 mM NaCl, 50 mM NaOAc, 25 mM MgCl
25 mg/mL BSA, 25 mM guanidine-HCl
,
2
Agilent D5000 ScreenTape Quick Guide for TapeStation Systems
D5000 ScreenTape Assay for TapeStation Systems
Storage Conditions
• Reagent vials and ScreenTape devices: 2 – 8 °C (36 – 46 °F).
• Store partially used ScreenTape devices upright at 2 – 8 °C (36 – 46 °F) for a maximum of 2 weeks.
• Never freeze ScreenTape devices. Discard any accidentally frozen ScreenTape devices.
Kit Components
Part Number Name Color Amount
5067-5588 D5000 ScreenTape 7 ScreenTape devices
5067-5589 D5000 Reagents
• D5000 Ladder
• D5000 Sample Buffer
5067-5590 D5000 Ladder 1 vial, 10 µL
2 vials
10 µL
1300 µL
Limited Use Label License
Some products within this assay contain SYBR™ Green I, which is licensed from Life Technologies Corporation for use
in research and development only. SYBR™ is a registered trademark of Life Technologies Corporation.
For Research Use Only
Not for use in Diagnostic Procedures.
Additional Material Required for Analysis with the TapeStation Systems
• Loading tips (5067-5598, 1 pk or 5067-5599, 10 pk)
• Optical Tube 8x Strip (401428) and Optical Tube Cap 8x Strip (401425)
• Vortex mixer IKA MS3 with 96-well sample plate adapter
• 96-well sample plates (5042-8502) and 96-well Plate Foil Seal (5067-5154) (4200 TapeStation systems only)
Additional Equipment Required (Not Supplied)
• Volumetric micropipettes for handling volumes from 1 to 15 µL
• Centrifuges for tube strips and 96-well sample plates
WAR NI NG
CA UTI ON
2
Toxic agents
Refer to product material safety datasheets for further information.
When working with the ScreenTape assay follow the appropriate safety procedures such as
wearing safety goggles, laboratory gloves and protective clothing.
Damage to the TapeStation systems
Only use the recommended consumables and reagents with the TapeStation systems.
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