Agilent Cell-free DNA ScreenTape Assay Quick Start Guide
Cell-free DNA ScreenTape Assay for TapeStation Systems
Quick Guide
The Agilent 4150 (G2992AA) and 4200 (G2991AA and G2991BA) TapeStation systems are automated platforms
for scalable, flexible, fast, and reliable electrophoresis of nucleic acids.
This Quick Guide is intended for use with the Agilent 4150 and 4200 TapeStation systems only. The Agilent Cell-free
DNA ScreenTape assay is exclusively available for use with the Agilent 4150 or 4200 TapeStation systems.
The Cell-free DNA ScreenTape assay is designed for analyzing cell-free DNA samples and double-stranded DNA
molecules from 50 to 800 basepairs.
Specifications
Analytical specifications Cell-free DNA ScreenTape assay
Sizing range 50 – 800 bp
Sensitivity
Sizing precision
Sizing accuracy
Quantitative precision
Quantitative accuracy
Quantitative range 100 – 4000 pg/µL
%cfDNA functional range 100 – 5000 pg/µL
Maximum buffer concentration in
sample
Physical specifications
Analysis time 16 samples: <25 min, 96 samples: <150 min
Samples per consumable 16
Sample volume required 2 µL
Kit stability 6 months
Kit size 112 samples
1
2
3
1
2
2,3
2
2
Signal-to-noise >3 (single peak)
Determined using the Cell-free DNA Ladder as sample
Sizing Accuracy for analysis with electronic ladder: ±20 %
20 pg/µL
10 % CV
±15 %
15 % CV
±20 %
25 mM NaCl, 25 mM KCl, 3 mM EDTA, 0.1 % NaN
ethanol, 10 % 2-propanol
, 5 mM phosphate buffer, 10 %
3
Agilent Cell-free DNA ScreenTape Quick Guide for TapeStation Systems
Cell-free DNA ScreenTape Assay for TapeStation Systems
Storage Conditions
• Reagent vials and ScreenTape devices: 2 – 8 °C (36 – 46 °F).
• Store partially used ScreenTape devices upright at 2 – 8 °C (36 – 46 °F) for a maximum of 2 weeks.
• Never freeze ScreenTape devices. Discard any accidentally frozen ScreenTape devices.
Kit Components
Part Number Name Color Amount
5067-5630 Cell-free DNA ScreenTape 7 ScreenTape devices
5067-5631 Cell-free DNA Reagents
• Cell-free DNA Ladder
• Cell-free DNA Sample Buffer
5067-5632 Cell-free DNA Ladder 1 vial, 50 µL
5067-5633 Cell-free DNA Sample Buffer 1 vial, 300 µL
2 vials
50 µL
300 µL
Limited Use Label License
Some products within this assay contain SYBR™ Green I, which is licensed from Life Technologies Corporation for use
in research and development only. SYBR™ is a registered trademark of Life Technologies Corporation.
For Research Use Only
Not for use in Diagnostic Procedures.
Additional Material Required for Analysis with the TapeStation Systems
• Loading tips (5067-5598, 1 pk or 5067-5599, 10 pk)
• Optical Tube 8x Strip (401428) and Optical Tube Cap 8x Strip (401425)
• Vortex mixer IKA MS3 with 96-well sample plate adapter
• 96-well sample plates (5042-8502) and 96-well Plate Foil Seal (5067-5154) (4200 TapeStation systems only)
Additional Equipment Required (Not Supplied)
• Volumetric micropipettes for handling volumes from 1 to 15 µL
• Centrifuges for tube strips and 96-well sample plates
WAR NI NG
CA UTI ON
2
Toxic agents
Refer to product material safety datasheets for further information.
When working with the ScreenTape assay follow the appropriate safety procedures such as
wearing safety goggles, laboratory gloves and protective clothing.
Damage to the TapeStation systems
Only use the recommended consumables and reagents with the TapeStation systems.
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