Agilent BenchCel Microplate Handler Original Instructions

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BenchCel Microplate Handler

User Guide

Original Instructions

Notices

WARNING

CAUTION

Manual Part Number

G5580-90000 Revision B

November 2020

No part of this manual may be reproduced in any form or by any means (including electronic storage and retrieval or translation into a foreign language) without prior agreement and written consent from Agilent Technologies, Inc. as governed by United States and international copyright laws.

Contact Information

Agilent Technologies Inc. Automation Solutions 5301 Stevens Creek Blvd. Santa Clara, CA 95051 USA

Web:

https://www.agilent.com

Contact page:

https://www.agilent.com/en/contactus/page

Documentation feedback:

documentation.automation@agilent.com

Acknowledgments

Microsoft® and Windows® are either registered trademarks or trademarks of the Microsoft Corporation in the United States and other countries.

Warranty

The material contained in this document is provided “as is,” and is subject to being changed, without notice, in future editions. Further, to the maximum extent permitted by applicable law, Agilent disclaims all warranties, either express or implied, with regard to this manual and any information contained herein, including but not limited to the implied warranties of merchantability and fitness for a particular purpose. Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing, use, or performance of this document or of any information contained herein. Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms, the warranty terms in the separate agreement shall control.

Technology Licenses

The hardware and/or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license.

Restricted Rights Legend

If software is for use in the performance of a U.S. Government prime contract or subcontract, Software is delivered and licensed as “Commercial computer software” as defined in DFAR 252.227-7014 (June 1995), or as a “commercial item” as defined in FAR 2.101(a) or as “Restricted computer software” as defined in FAR

52.227-19 (June 1987) or any equivalent agency regulation or contract clause. Use, duplication or disclosure of Software is subject to Agilent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as defined in FAR 52.22719(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 (June1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any technical data.

Safety Notices

A WARNING notice denotes a hazard. It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in personal injury or death. Do not proceed beyond a

WARNING notice until the indicated

conditions are fully understood and met.

CAUTION notice denotes a hazard.

A It calls attention to an operating procedure, practice, or the like that, if not correctly performed or adhered to, could result in damage to the product or loss of important data. Do not proceed beyond a the indicated conditions are fully understood and met.

CAUTION notice until

Contents

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v

About this guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi

Accessing product user information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . viii

1. Safety guidelines. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1

General safety information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2

Safety and regulatory compliance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3

Emergency stops . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Safety equipment and features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Potential safety hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

2. Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

BenchCel Microplate Handler description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

Hardware overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15

Integration options for workstations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Labware considerations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Software description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29

3. Installing BenchCel Microplate Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35

Installation workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Verifying laboratory requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

Unpacking BenchCel Microplate Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

Mounting the robot . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

About integrating devices in a BenchCel Workstation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47

Connecting the power source. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50

Connecting the pendant directly to the BenchCel device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51

Connecting the pendant through the Robot Disable Hub . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53

Connecting and disconnecting the air source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55

Connecting the computer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57

Installing the shield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61

4. Setting up the BenchCel device. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Setup workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64

Starting up and shutting down . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66

Creating BenchCel device in the VWorks software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68

Opening BenchCel Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73

Creating profiles . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

Initializing the device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82

Setting and managing teachpoints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84

iiiBenchCel Microplate Handler User Guide

Contents

5. Setting sensor thresholds. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99

Overview for setting sensor thresholds . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100

Calculating the Plate presence threshold. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102

Determining the optimum Orientation sensor offset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107

Calculating the Orientation threshold . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111

Worksheet for setting sensor thresholds. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113

6. Preparing for a run. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 115

Workflow for operating the BenchCel Microplate Handler . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116

Handling the labware racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117

Filling and emptying the labware racks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119

Installing and uninstalling the labware racks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123

Performing pre-run checks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128

7. Maintenance and troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129

Routine maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 130

Cleaning up after a protocol run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132

Replacing the fuse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 134

Hardware problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137

Software error messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 139

Diagnostic tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146

Adjusting the stacker gripper pressure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158

Reporting problems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162

A. Quick reference. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165

Rack-release button indicator light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166

BenchCel Diagnostics - Controls tab. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167

BenchCel Diagnostics - Jog/Teach tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 170

BenchCel Diagnostics - Labware tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173

BenchCel Diagnostics - General Settings tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 178

BenchCel Diagnostics - Profiles tab . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179

Teachpoint Details dialog box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182

iviv

BenchCel Microplate Handler User Guide

Preface

This preface contains the following topics:

• “About this guide” on page vi

• “Accessing product user information” on page viii

Preface

About this guide

Who should read this guide

This user guide is for people with the following job roles:

Job role Responsibilities

Installer Unpacks, installs, and tests the device before it is used.

Integrator Configures hardware and writes software.

Lab manager, administrator, or technician

Operator Performs the daily production work on the device and solves

What this guide covers

This guide describes the G5580A BenchCel Microplate Handler and earlier R-Series models. This guide covers installation, setup, operation, and maintenance.

This guide does not provide instructions for the following:

• Agilent devices that are integrated with the BenchCel Microplate Handler in a

workstation configuration, such as the Bravo Platform, PlateLoc Thermal Microplate Sealer, Microplate Labeler, and Labware MiniHub

• Third-party devices

For more information about these topics, see the relevant user guides for these products.

• Manages the automation system that contains the device

• Develops the applications that are run on the system

• Develops training materials and standard operating

procedures for operators

routine problems.

Software version

This guide is applicable for the following software:

• BenchCel Diagnostics 21.3.0, which is included in VWorks software 14.0 and later.

• BenchCel Diagnostics versions included in VWorks software 12.3 – 13.1.x.

What is new in this edition

and

Description See…

Added descriptions for VWorks software

14.0.

“Software description” on page 29

BenchCel Microplate Handler User Guide

Description See…

Preface

About this guide

Related guides

Updated the Robot Disable Hub descriptions to specify the 3-Port Robot Disable Hub.

Updated the instructions for creating the device file to include how to select the teachpoint for an integrated device.

Updated the profile and teachpoint descriptions and procedures to reflect changes introduced in VWorks software

14.0.

Updated the fuse replacement procedure to make the description consistent for both 115 V and 230 V power.

Added a description of the indicators on the Controls tab.

• “Safety equipment and features” on

page 8

• “Connecting the pendant through

the Robot Disable Hub” on page 53

“Specifying BenchCel accessibility at other devices” on page 72

• “Creating profiles” on page 76

• “Setting and managing teachpoints”

on page 84

• “BenchCel Diagnostics - Profiles

tab” on page 179

“Replacing the fuse” on page 134

“BenchCel Diagnostics - Controls tab” on page 167

You should use this guide in conjunction with the following guides:

• Automation Solutions Products General Safety Guide. Provides general safety

information and describes potential safety hazards that you might encounter when using Automation Solutions products. A copy of this safety guide is included with your shipment.

• VWorks software guides: – VWorks Automation Control Setup Guide. Explains how to define labware and

labware classes, liquid classes, and pipetting techniques. The VWorks 14.0 version also explains the VWorks compliance features and

how to set up an experiments databases.

– VWorks Automation Control User Guide. Explains how to create protocols, and

set task parameters for each device in the system.

• BenchCel Microplate Handler Quick Guide. The quick guide summarizes the

instructions in this user guide.

• Agilent Technologies device user guides. These guides explain how to set up and

use the devices that you integrate with the BenchCel Microplate Handler, such as the Bravo Platform User Guide.

• Third-party device user documents, if applicable. These guides explain how to set

up and use the third-party devices.

BenchCel Microplate Handler User Guide

vii

Preface

Accessing product user information

About this topic

This topic describes the different formats of user information and explains how to access it for the Agilent Automation Solutions products.

Where to find user information

The user information is available in the following locations:

• Knowledge base. The help system for the Automation Solutions products is

available from:

– Help menu within the VWorks software: Select Help > Knowledge Base or press

F1.

– From the Windows desktop: Select Start ( ) > All Apps > Agilent

Technologies > VWorks Knowledge Base.

For guidelines on using the VWorks context-sensitive help and knowledge base features, see Using the knowledge base, below.

• PDF files. The PDF files of the user guides are installed with the VWorks software

(C:\Program Files (x86)\Agilent Technologies\VWorks\UserGuides) and are available in the VWorks Knowledge Base.

• Website. You can search the online VWorks Knowledge Base or download the latest

version of any PDF file from the Agilent website at www.agilent.com/chem/askb.

Accessing safety information

Safety information for the Agilent Automation Solutions devices appears in the

Automation Solutions Products General Safety Guide and in the corresponding device

safety guide or user guide.

You can also search the knowledge base or the PDF files for safety information.

Using the knowledge base

Knowledge base topics are displayed using web browser software such as Microsoft Edge.

Note: If you want to use Internet Explorer to display the topics, you might have to allow local files to run active content (scripts and ActiveX controls). To do this, in Internet Explorer, open the Internet Options dialog box. Click the Advanced tab, locate the Security section, and select Allow active content to run in files on my computer.

viii

BenchCel Microplate Handler User Guide

Accessing product user information

Opening the help topic for an area in the VWorks window

Preface

To access the context-sensitive help feature:

1 In the main window of the VWorks software, click the help button . The pointer

changes to . Notice that the different icons or areas are highlighted as you move the pointer over them.

2 Click an icon or area of interest. The relevant topic or document opens.

BenchCel Microplate Handler User Guide

Preface

Accessing product user information

Features in the Knowledge Base window

Step For this task...

1 Contents pane. Lists all the books and the table of contents of the books.

2 Search. Allows you to search the Knowledge Base (all products or selected products) using

keywords.

3 Topic area. Displays the selected online help topic.

4 Navigation buttons. Enable you to navigate through the next or previous topics listed in the Contents

tab.

5 Toolbar buttons: Enable you to:

• Expand or collapse all the sections in a topic that has drop-down headings.

• Print the topic.

• Send feedback by email for a given topic.

BenchCel Microplate Handler User Guide

1 Safety guidelines

This chapter contains the following topics for the BenchCel Microplate Handler:

• “General safety information” on page 2

• “Safety and regulatory compliance” on page 3

• “Emergency stops” on page 5

• “Safety equipment and features” on page 8

• “Potential safety hazards” on page 10

1 Safety guidelines

WARNING

General safety information

Before installing and using the device

Before using the BenchCel Microplate Handler, make sure that you are properly trained in:

• General laboratory safety

• The correct and safe operation of the BenchCel Microplate Handler

• The correct and safe operation of devices used in combination with the BenchCel

Microplate Handler

Changing or modifying the BenchCel safety equipment may prevent the safe operation of the BenchCel Microplate Handler, invalidate its safety compliance, and lead to personal injury or property damage. Any customer who does not use the supplied safety equipment or who modifies the supplied safety equipment assumes full responsibility for providing an appropriate level of safety for its operators and for providing the applicable safety compliance marking and documentation.

General safety precautions

For the general safety precautions, the intended product use statement, and the list of safety labels, see the Automation Solutions Products General Safety Guide.

The following figure shows the location of the safety label on the BenchCel Microplate Handler.

Figure

Safety label location (front view and side view)

BenchCel Microplate Handler User Guide

Related information

For information about... See...

1 Safety guidelines

Safety and regulatory compliance

General precautions, intended product use statement, and list of safety labels

Regulatory compliance “Safety and regulatory compliance” on

Stopping in an emergency “Emergency stops” on page 5

Safety equipment “Safety equipment and features” on page 8

Potential hazards specific to the BenchCel Microplate Handler

Safety and regulatory compliance

About this topic

This topic lists the compliance regulations and standards for the BenchCel Microplate Handler.

Note: Earlier models of the BenchCel Microplate Handler may not comply with all the standards listed in this topic.

Automation Solutions Products General Safety Guide

page 3

“Potential safety hazards” on page 10

Compliance information

The BenchCel Microplate Handler is considered partly completed machinery as defined by the Machinery Directive of the European Union. Therefore, the BenchCel Microplate Handler is provided with a Declaration of Incorporation and no CE marking. The BenchCel Microplate Handler is intended to be integrated into a conforming completed machine (workstation). A customer in the European Union (EU) that acquires a standalone BenchCel Microplate Handler is responsible for this integration and for CE marking the completed workstation. The BenchCel Microplate Handler is designed to comply with the standards listed in the table below.

Unless specifically requested by the customer, BenchCel Workstations provided by Agilent Technologies are configured to conform to the Machinery Directive and bear the CE marking. The Declaration of Conformity shipped with the BenchCel Workstation describes the specific configuration that has been evaluated for conformity. Any deviations from the specified configuration may invalidate the Declaration of Conformity. See the Declaration of Conformity for details.

An EU customer that requests Agilent Technologies to provide a nonconforming BenchCel Workstation must assume responsibility as the manufacturer for completing the workstation in conformity with the applicable directives, including the Machinery Directive. In the case of a nonconforming BenchCel Workstation, each component that Agilent Technologies supplies will carry a Declaration of Conformity or Declaration of Incorporation, as appropriate, to be used by the manufacturer to compile the technical construction file.

BenchCel Microplate Handler User Guide

1 Safety guidelines

Safety and regulatory compliance

Regulatory Compliance Standard

EMC

European Union EMC Directive 2014/30/EU

Canada ICES/NMB-001:2006

Australia/New Zealand AS/NZS CISPR 11:2011

RoHS

European Union RoHS Directive 2011/65/EU

Safety

European Union Machinery Directive 2006/42/EC

IEC 61326-1:2012 / EN 61326-1:2013

Low Voltage Directive 2014/35/EU

IEC 61010-1:2001 / EN61010-1:2001

IEC 61010-2-081:2001+A1:2003 /

EN 61010-2-081:2002+A1:2003

Canada CAN/CSA-C22.2 No. 61010-1-04

CAN/CSA-C22.2 No. 61010-2-081-04

USA ANSI/UL 61010-1:2004

Electromagnetic compatibility

If the BenchCel Microplate Handler causes interference with radio or television reception, which can be determined by turning the device off and on, try one or more of the following measures:

• Relocate the radio or television antenna.

• Move the device away from the radio or television.

• Plug the device into a different electrical outlet, so that the device and the radio or

television are on separate electrical circuits.

• Make sure that all peripheral devices are also certified.

• Make sure that appropriate cables are used to connect the device to peripheral

equipment.

• Consult your equipment dealer, Agilent Technologies, or an experienced technician

for assistance.

Changes or modifications not expressly approved by Agilent Technologies could void the user's authority to operate the equipment.

Sound emission declaration

Sound pressure: Lp < 70 dB according to EN ISO 779:2010.

BenchCel Microplate Handler User Guide

Schalldruckpegel: Lp < 70 dB nach EN ISO 779:2010.

WARNING

CAUTION

WARNING

Related information

For more information about… See…

General safety and labels “General safety information” on page 2

Safety hazards “Potential safety hazards” on page 10

Stopping in an emergency “Emergency stops” on page 5

Safety equipment “Safety equipment and features” on page 8

Emergency stops

The BenchCel Microplate Handler is equipped with an interlock circuit and emergencystop pendant.

1 Safety guidelines

Emergency stops

To avoid potential injury, Agilent recommends that you install a main emergency stop button (Robot Disable Hub) that will stop the BenchCel robot and all other devices on the safety interlock circuit simultaneously. In addition, all operators must be instructed in the emergency stop procedure.

You might not be able to resume a protocol after an emergency stop. Do not use an emergency stop to pause a run. To pause and continue a run, use the appropriate commands in the automation software.

Stopping in an emergency

After pressing the emergency-stop button, the robot arm might have momentum and continue to move until it comes to the end of its travel in the x-axis, z-axis, or thetaaxis or until it bumps into an obstacle. Stay clear of the robot until it stops moving.

To perform an emergency stop:

Press the red button on the pendant. The safety interlock circuit is interrupted, disabling the robot motors. The BenchCel Microplate Handler operation stops.

BenchCel Microplate Handler User Guide

1 Safety guidelines

Emergency stops

Recovering from an emergency stop

After you press the button on the emergency-stop pendant, the robot stops. One of the following occurs:

• If you stopped a protocol run, a dialog box opens in the VWorks software.

• If you stopped the robot while diagnosing problems in BenchCel Diagnostics, a

motor-disable message appears on the screen.

Use the following procedure to recover in either case.

To recover the BenchCel device after an emergency stop:

1 If the robot dropped labware before or during the emergency stop, remove labware

that was dropped. Also remove labware at teachpoints or other locations.

2 If the BenchCel robot attempted to place labware at a location that was not free, a

collision might have occurred resulting in misalignment of the robot gripper. Check the robot gripper alignment:

a Move the robot arms so that they are perpendicular to the x-axis. b Make sure the bottom of the robot gripper pads are perpendicular to the robot

arms. If they are not, contact Agilent Technical Support.

Figure

Gripper alignment: (A) correct alignment, and (B) incorrect alignment

A) Correct alignment

B) Incorrect - misaligned

3 To re-enable the motors:

a At the emergency-stop pendant, turn the button clockwise to release it.

BenchCel Microplate Handler User Guide

1 Safety guidelines

Emergency stops

b In the VWorks software message dialog box, select an appropriate option to

continue:

Selection Description

Diagnostics Opens the BenchCel Diagnostics dialog box. See step 4.

Note: This selection is available only when you are in the middle of a protocol run and not while you are in BenchCel Diagnostics.

Retry Attempts to restart the current command or task in the

run.

Ignore and continue

Abort Aborts the current command or task in the run. Select

Ignores the current command or task and continues to the next command or task in the protocol sequence.

Abort if you have determined that the protocol run is not recoverable.

4 Optional. In BenchCel Diagnostics, use the available commands to manually move

If a physical crash occurred, always start BenchCel Diagnostics to home the robot and verify teachpoints.

Related information

For information about… See…

Pausing and resuming protocol runs VWorks Automation Control User Guide

Using commands in BenchCel Diagnostics

Complete list of available commands in BenchCel Diagnostics

For a full description of the selections, see the VWorks Automation Control User

Guide.

the robot or other components, including:

• Release the microplate that the robot is currently holding.

• Upstack the microplate that the robot is currently holding.

• Replace the lid on the microplate.

• Home the robot.

• Verify teachpoints.

For details, see “Diagnostic tools” on page 146.

“Diagnostic tools” on page 146

“Quick reference” on page 165

Reporting a problem “Reporting problems” on page 162

BenchCel Microplate Handler User Guide

1 Safety guidelines

WARNING

Safety equipment and features

About this topic

This topic describes the safety equipment and features of the BenchCel Microplate Handler that are required to protect operators from moving-parts hazards.

Shields

Make sure the BenchCel device is enclosed in the supplied shield. The shield restricts access to the BenchCel robot while the BenchCel Microplate Handler is operating.

Figure

BenchCel front shield

Additional shields may be required on devices that are integrated with the BenchCel Microplate Handler, such as the Bravo Platform.

In addition, a light curtain connected to the safety interlock circuit may be required.

BenchCel Microplate Handler User Guide

WARNING

To reduce the risk of injury from moving parts, ensure that the BenchCel shield and

WARNING

the shields for any integrated devices are installed on the workstation.

Operating the Bravo Platform or Microplate Centrifuge with Loader without a safety shield or a light curtain connected to the safety interlock circuit increases the risk of injury.

Safety interlock circuit

The BenchCel Microplate Handler has a safety interlock circuit that is designed to protect operators from moving-parts hazards. The safety interlock circuit must be closed for the device to operate. The pendant connects to the safety interlock circuit. Pressing the red button on the pendant trips the interlock circuit and causes the motion of the BenchCel operation to stop.

1 Safety guidelines

Safety equipment and features

Figure

When the BenchCel device is integrated with another device that has a safety interlock circuit, such as the Bravo Platform, Agilent Technologies uses the Robot Disable Hub to connect all the devices on the interlock circuit. Pressing the red button on a pendant or interrupting a light curtain connected to the Robot Disable Hub will trip the safety interlock circuit and stop all the devices connected to the circuit.

Figure

Related information

For information about… See…

Emergency-stop pendant

Robot Disable Hub

Pendants Power

Devices

Light Curtain

BenchCel Microplate Handler User Guide

Stopping in an emergency “Emergency stops” on page 5

Product compliance “Safety and regulatory compliance” on

page 3

1 Safety guidelines

WARNING

Potential safety hazards

For information about… See…

Moving parts and other hazards “Potential safety hazards” on page 10

Safety labels and general information “General safety information” on page 2

Reporting a problem “Reporting problems” on page 162

Potential safety hazards

About this topic

This topic contains precautions about potential hazards that are specific to the BenchCel Microplate Handler. In addition to the following potential hazards, make sure that you understand how to avoid the general hazards described in the Automation

Solutions Products General Safety Guide.

The potential hazards that you can encounter when using the BenchCel Microplate Handler include the following:

• “Moving parts” on page 10

• “Sharp edges and pinch hazards” on page 11

• “Infrared LED injury hazard” on page 11

• “Static electricity” on page 12

• “Improper access or use” on page 12

• “Lifting hazard” on page 12

Moving parts

To minimize potential injury, the BenchCel device is designed to stop immediately if the robot head hits an obstacle while it is in operation. However, be aware that the robot moves with considerable force in the vertical or z-axis direction and could pierce your skin with one of its grippers.

Not all circumstances can be foreseen and serious injury is possible. It is the responsibility of every operator to follow warnings and safety labels and keep out of the robot’s workspace whenever it is likely to move.

The BenchCel Microplate Handler has moving parts that can injure you if you deviate from the procedures given in this guide. Keep your fingers, hair, clothing, and jewelry away from the BenchCel Microplate Handler while it is in motion.

To avoid potential injury, do not touch the BenchCel Microplate Handler as you start the software. The robot head moves when the device initializes.

BenchCel Microplate Handler User Guide

WARNING

Never touch any of the moving parts or attempt to remove or add labware while the

WARNING

BenchCel Microplate Handler is in operation. The robot head moves with considerable force and can cause pinching, piercing, or bruising injury if you are in the path of the robot head or gripper.

Sharp edges and pinch hazards

Pinch hazard! Keep your fingers out of the path of the labware racks when you mount the racks on the BenchCel device.

Use the rack handle to carry the labware racks. Do not hold a rack by the interior edges. The interior edges might have sharp surfaces that could cause cuts if handled improperly.

Figure Sharp surfaces on labware rack

1 Safety guidelines

Potential safety hazards

Infrared LED injury hazard

BenchCel Microplate Handler User Guide

Do not stare directly at the light-emitting diodes (LEDs) inside the stacker heads when the device is on. Such exposure to the LEDs can cause eye injury.

Each BenchCel stacker head contains seven infrared LEDs that detect the presence of a labware rack, microplates, and microplate notches. The LEDs are capable of dissipating 100 mW of power. Do not stare directly at the LEDs when the BenchCel Microplate Handler is turned on.

1 Safety guidelines

CAUTION

WARNING

CAUTION

Potential safety hazards

Static electricity

Improper access or use

The rack-release button at the top of the stacker head on the BenchCel device is sensitive to static electricity. Static electricity can cause a potential loss of motor control and loss of communication with the BenchCel device. Ensure that you discharge any potential static electricity before touching the rack-release button. To discharge static electricity, you can touch the white painted or metal surface of the BenchCel device before touching the rack-release button.

Improper use by an untrained user could damage the BenchCel Microplate Handler. For example, the robot gripper could collide with a stacker if a teachpoint is not defined properly.

Ensure that only fully trained BenchCel administrators have access to the user account passwords.

Lifting hazard

The BenchCel device without labware racks weighs 28 – 51 kg (61.7 – 112.4 lb), depending on the configuration. Attempting to lift or move the BenchCel device without assistance could cause personal injury. Request assistance and use proper lifting techniques when lifting or moving the device.

If the BenchCel Microplate Handler is incorrectly packaged and moved by someone other than an Agilent employee, the device may be damaged. Such damage is not covered by the warranty.

Before moving a BenchCel Microplate Handler, ensure the new location meets the laboratory requirements.

Related information

For more information about… See…

General safety “General safety information” on page 2

Stopping in an emergency “Emergency stops” on page 5

Shields, pendants, and safety interlock circuit

“Safety equipment and features” on page 8

Locations of hardware components “Hardware overview” on page 15

Site laboratory requirements “Verifying laboratory requirements” on

page 37

Reporting problems with the BenchCel Microplate Handler

“Reporting problems” on page 162

BenchCel Microplate Handler User Guide

2Introduction

This chapter contains the following topics:

• “BenchCel Microplate Handler description” on page 14

• “Hardware overview” on page 15

• “Integration options for workstations” on page 25

• “Labware considerations” on page 27

• “Software description” on page 29

2 Introduction

BenchCel Microplate Handler description

Description

The BenchCel Microplate Handler is a microplate-processing automation device that:

• Stores stacks of labware (microplates, tip boxes, and tube racks) to be processed

during a protocol run.

• Moves labware to and from integrated devices such as the Bravo Platform,

PlateLoc Thermal Microplate Sealer, or Microplate Labeler for processing.

Figure

BenchCel Microplate Handler with safety shield and two labware racks

Configurations

The BenchCel Microplate Handler is available in three options:

• BenchCel 2R has two stackers that support two labware racks

• BenchCel 4R has four stackers that support four labware racks

• BenchCel 6R has six stackers that support six labware racks

The BenchCel Microplate Handler is included in the following standard Agilent workstations:

• G5590A BenchCel Workstation

• G5591A Bravo BenchCel Workstation

BenchCel Microplate Handler User Guide

Related information

WARNING

For more information about… See…

2 Introduction

Hardware overview

Agilent devices that you can integrate in a BenchCel Workstation

Hardware component description “Hardware overview” on page 15

Automation-ready labware “Labware considerations” on page 27

Safety information “Safety guidelines” on page 1

Hardware overview

About this topic

This topic describes the hardware features of the BenchCel device. Note that the figures in this topic show a BenchCel 2R device with two stackers that support two labware racks. All the major components and functions are the same for the BenchCel 4R (four stackers) and BenchCel 6R (six stackers).

Do not operate the BenchCel Microplate Handler without the safety shield. Doing so increases the risk of injury.

“Integration options for workstations” on page 25

BenchCel Microplate Handler User Guide

2 Introduction

Hardware overview

Front view

Item Feature Description

1 Labware

rack

2 Stacker

head

The accessory that stores labware to be processed in a run. For details, see “Labware racks” on page 24.

The structure at which:

• A labware rack is loaded. Two sensors inside of each

stacker head detect the presence of the racks.

• A microplate is checked for type and orientation using a

plate-presence sensor and four plate-orientation sensors.

• A microplate is lowered into the stacker grippers to begin

a run.

See “Stacker head” on page 20 for the location and detailed descriptions of the sensors.

3 Air

pressure regulator

The knob that you turn to adjust the air pressure inside the device. Compressed air is used to move components inside the stacker heads. Each regulator controls the air pressure to the two adjacent stacker heads. For details, see “Adjusting the

stacker gripper pressure” on page 158.

4 Safety

shield

The clear panel that is installed on the front of the BenchCel device to prevent access while it is in operation.

BenchCel Microplate Handler User Guide

2 Introduction

theta-axis

Hardware overview

Item Feature Description

5 Pendant The component that is part of the safety interlock circuit,

which must be closed for the BenchCel device to operate. Pressing the raised button on the pendant interrupts the safety circuit and disables the robot motors. Use this method of stopping the robot for emergencies only.

6 Rack-

release button

7 Robot

head

The button that unlocks the rack for removal. The rack-release button at the top of each stacker head displays different colors to indicate the state of the stacker head:

• Green. The labware rack is installed correctly on the

BenchCel device and the microplates are unloaded. The stack of microplates are ready for processing or you can unlock and remove the labware rack.

• Flashing green. The labware rack is unlocked and can be

removed.

• Blue. The stack of microplates is loaded. You cannot

unlock and remove the labware rack.

• Red. The clamps are open without a rack installed. Do not

install a rack until the clamps are closed.

The component that moves horizontally along the x-axis and vertically along the z-axis.

z-axis

x-axis

8 Robot

arms

Two parallel structures that are attached to and rotate about the robot head along the theta-axis.

BenchCel Microplate Handler User Guide

2 Introduction

Hardware overview

Item Feature Description

Back view

9 Robot

grippers

The structures inside the robot arms that close and open to hold and release a microplate. Using the provided software, you can adjust the distance between the grippers to hold a microplate loosely or tightly.

Robot grippers

2 3

BenchCel Microplate Handler User Guide

Item Feature Description

2 Introduction

Hardware overview

1 Air-input

fitting

2 Power

Connects the air tubing to the BenchCel device. Compressed air is used to actuate components inside the stacker head.

Turns on or off the power to the BenchCel device.

switch

3 AC power

Connects the power cord to the BenchCel device.

entry

4 Ethernet

port

Connects the Ethernet cable from the controlling computer to the BenchCel device to allow communication between the computer and the device. Use this port as an alternative to the serial connection.

5 Serial port Connects the serial cable from the controlling computer to the

BenchCel device to allow communication between the computer and the device. Use this port as an alternative to the Ethernet connection.

6 Pendant

port

Connects the emergency-stop pendant to the safety interlock circuit. The connection is made either directly or through an emergency-stop control, such as the Robot Disable Hub.

IMPORTANT The safety interlock circuit must be closed for

the BenchCel Microplate Handler to operate.

BenchCel Microplate Handler User Guide

2 Introduction

Hardware overview

Stacker head

At the top of the BenchCel device are stacker heads that contain infrared sensors and mechanical components that load and unload microplates during operation.

The following table lists and describes the various components inside the stacker head.

BenchCel Microplate Handler User Guide

Feature Description

Plate-presence sensor

Rack-presence sensors

2 Introduction

Hardware overview

Plate-presence sensor

Rack-presence sensors

Detects the presence of a microplate in the stack. One platepresence sensor is on the back wall of each stacker head.

Detect the presence of labware racks. Two rack sensors are on the back wall of each stacker head.

BenchCel Microplate Handler User Guide

2 Introduction

A B C D E F G H I J K L M N O P

1234

5678

9 101112131415161718192021222324

Notches

Plate-orientation sensor

A B C D E F G H I J K L M N O P

1234

5678

9 101112131415161718192021222324

Notches

Clamps

Hardware overview

Feature Description

Plate-orientation sensors

Detect the presence of notches in microplates. Four sensors are in the inside corners of each stacker head. For details of how the sensors work, see “Setting sensor thresholds” on page 99.

Clamps Close and open the grippers at the bottom of the labware rack to

hold and release microplates during loading, unloading, downstacking, and upstacking processes. Two clamps are inside each stacker head. Compressed air is used to open and close the clamps.

BenchCel Microplate Handler User Guide

2 Introduction

Shelves

Hardware overview

Feature Description

Shelves Provide leveling surfaces for the microplates, thus ensuring

accurate robot gripping, during the downstacking process. Two shelves (four leveling surfaces) are inside each stacker head. Compressed air is used to move the shelves.

BenchCel Microplate Handler User Guide

2 Introduction

Hardware overview

Labware racks

The labware racks are available in two models:

• Front-load rack in 250-mm and 660-mm sizes

• Top-load rack in 660-mm and 860-mm sizes

Both rack models have the following basic components.

Figure

Front-load rack components

Item Name Description

1 Carrying handle The top-load and front-load racks have fold-down

carrying handles.

2 Tabs A pair of vertical tabs are located at the bottom on the

rack sides. The tabs insert into slots on the device when you mount the rack onto the device.

3 Stacker grippers A gripper is located on the interior bottom of each tab.

The pair of grippers hold a microplate during the labware loading, unloading, downstacking, and upstacking processes. A clamp in the device opens and closes the grippers.

BenchCel Microplate Handler User Guide

2 Introduction

Microplate

Integration options for workstations

The following figure shows a closeup view of a labware rack, with the stacker grippers holding a microplate. The front wall of the rack is not shown to reveal the stacker grippers that are hidden from view.

Figure

Related information

For more information about… See…

Automation-ready labware “Labware considerations” on page 27

Software that controls the BenchCel Microplate Handler

Safety information “Safety guidelines” on page 1

Installation requirements “Verifying laboratory requirements” on

Labware rack closeup view showing microplate held by the stacker grippers

“Software description” on page 29

page 37

Integration options for workstations

About this topic

You can integrate Agilent devices with the BenchCel Microplate Handler to create a BenchCel Workstation. The BenchCel robot can move labware to and from these devices as specified by the protocol you create.

This topic lists some of the devices that can be integrated with the BenchCel Microplate Handler.

Agilent devices

The following figure shows an example of a BenchCel Workstation.

BenchCel Microplate Handler User Guide

2 Introduction

Integration options for workstations

Figure

six stackers, and (

Example of BenchCel Workstation: (1) computer, (2) Bravo Platform, (3) BenchCel with

4) Labware MiniHub

The following Agilent devices can be integrated with the BenchCel Microplate Handler.

Item Device Description

Related information

For more information about… See…

BenchCel Microplate Handler features “Hardware overview” on page 15

Safety information “Safety guidelines” on page 1

Installation requirements “Installing BenchCel Microplate Handler” on

1 Bravo Platform Dispenses liquids.

2 Labware MiniHub Stores labware.

3 Microplate Labeler Prints barcodes and applies the

barcode labels to microplates.

4 Microplate Centrifuge with

Centrifuges microplates.

Automated Loader

5 PlateLoc Thermal Microplate

Applies seal on microplates.

Sealer

page 35

BenchCel Microplate Handler User Guide

Labware considerations

Microplate

About this topic

This topic provides guidelines for selecting automation-ready labware for use in the BenchCel Microplate Handler.

Acceptable microplates

The BenchCel device is designed to handle labware that comply with the standards ANSI/SLAS 1-2004 (R2012) through ANSI/SLAS 4-2004 (R2012). For use of nonconforming labware, contact Agilent Technical Support.

The BenchCel device uses gripping mechanisms to hold microplates securely and repeatably in the labware rack and in the robot arms. The BenchCel device typically holds the microplates halfway between the top of the microplate and the top of the microplate skirt (5 to 10 mm above the bottom of the microplate).

In the following figure, notice the gripper-microplate contact point.

2 Introduction

Labware considerations

Figure

Lidded microplates

Microplates that do not have lids or have shallow lids (lids that do not reach the microplate skirt) provide enough clearance to allow secure and repeatable gripping. Microplates with deeper lids can be more challenging, because the microplate must be held by the skirt. If the skirt is too flexible, the stacker grippers will bend the skirt. The bent skirt can grip the microplate lid stacked beneath, inadvertently removing the lid.

Note: Some labware vendors might offer alternative lids that are shallower. Contact the vendor for details.

Labware rack closeup view showing microplate held by the stacker grippers

BenchCel Microplate Handler User Guide

2 Introduction

Labware considerations

Figure

Lidded microplate examples

Challenging microplate characteristics

Microplates that have the following characteristics might require additional setup time to ensure repeatable performance for the BenchCel device:

• Microplate material. Although you can adjust the robot grip distance to compensate

for a microplate’s flexibility, some microplates are too soft and tend to bend in the robot grippers or become warped after thermal cycling. (for example, low-profile polypropylene PCR microplates).

• Manufacturing variance. Gross variations in microplate dimensions can reduce

repeatability of secure gripping. In addition, because the BenchCel device uses reflected light to sense microplate presence and orientation, variations in the reflective properties of the microplates can affect optimal operation.

• Microplate design. Some microplates have special features specifically designed

for particular instruments but are not optimized for the BenchCel device.

• Thermal cycling effects. Microplates that have been through thermal cycling might

become warped.

• Tall labware. Especially tall tube racks and tip boxes that are taller than 65 mm

might pose challenges in the BenchCel device. Contact Agilent Technical Support about acceptable tall labware.

• Extra long lid. Some microplates that have lids that extend past the microplate skirt

tend to pose challenges for the BenchCel device. Contact Agilent Technical Support for guidance.

No lid: excellent gripper clearance

Shallow lid: good gripper clearance

Deep lid: no gripper clearance, must be held by the skirt

Related information

For more information about… See…

Defining labware in the software VWorks Automation Control Setup Guide

Location of plate-orientation sensors “Stacker head” on page 20

How the plate-orientation sensors work

“Setting sensor thresholds” on page 99

BenchCel Microplate Handler User Guide

For more information about… See…

BenchCel Microplate Handler features “Hardware overview” on page 15

Safety information “Safety guidelines” on page 1

Installation requirements “Installing BenchCel Microplate Handler” on

Software description

About this topic

This topic describes the software that you can use to set up, control, and troubleshoot the BenchCel Microplate Handler.

VWorks software 13.1.x

2 Introduction

Software description

page 35

VWorks 13.1.x enables you to:

• Set up user accounts and privileges

• Set up your devices

• Define labware, liquid classes, and pipetting techniques

• Manage labware inventory

• Create protocols and forms, and run the protocols

VWorks 13.1.x uses the Windows Registry to store records, such as labware definitions and device profiles, and uses the local file system to store VWorks files, such as device files and protocols.

BenchCel Microplate Handler User Guide

2 Introduction

Software description

VWorks software 14.0 and later versions

VWorks 14.0 and later versions are available as VWorks Plus and VWorks Standard editions. Both editions consist of Agilent OpenLab components and VWorks software. VWorks Plus includes features that support compliance with Part 11 of Title 21 of the Code of Federal Regulations (21 CFR Part 11). You use the VWorks software to control your automation devices

OpenLab components

• Control Panel and Shared Services. Control Panel is the user interface for Shared

Services, which are set of administrative services. An administrator uses Control Panel to configure VWorks user access, software licenses, and storage.

• Storage types. Shared Services uses a different storage type for each VWorks

edition:

– VWorks Plus. Uses Content Management to store VWorks files, records of

interest, and audit trails. The files are stored in a predefined VWorks Project folder in the Content Management repository. A system administrator can use the Content Browser to view and edit the project structure and contents.

– VWorks Standard. Uses the local file system. Shared Services stores the files in

a predefined VWorks Project folder.

/VWorks Projects/VWorks

Note: Unlike earlier versions, the Windows Registry is not used for storing records, such as labware definitions and device profiles.

VWorks software

You use the VWorks software to do the following:

• VWorks Plus only. Log audit trails and manage record states

• Define labware, liquid classes, and pipetting techniques

• Manage labware inventory (storage devices only)

• Track experiments, if applicable

• Set up devices

• Create protocols and forms, and run the protocols

BenchCel Microplate Handler User Guide

2 Introduction

Software description

Figure

VWorks software window

BenchCel ActiveX control

You can use the BenchCel ActiveX control instead of the VWorks software to control the BenchCel Microplate Handler. The BenchCel ActiveX control enables the BenchCel device to interact with a third-party lab automation system.

BenchCel Diagnostics software

Accessed through the VWorks software or the BenchCel ActiveX control, Diagnostics enables you to do the following:

• Create and manage profiles. The software uses the information in the profile to

communicate between the BenchCel device and the controlling computer. You create profiles using the Profiles tab in BenchCel Diagnostics when you set up the BenchCel Microplate Handler.

BenchCel Microplate Handler User Guide

2 Introduction

Software description

Figure

BenchCel Diagnostics Profiles tab

Note: In VWorks v14.0 and later versions, BenchCel Diagnostics includes an activity log for the current session in a box at the bottom of the window. The Profiles tab also includes a table that displays variables that were modified since the last was last saved. The VWorks software records these events and stores the information in the Main log. For details on the logs, see the VWorks

Automation Control User Guide.

• Set and edit teachpoints. Teachpoints are locations that the BenchCel robot will go

to and from during a protocol run. You set teachpoints using the Diagnostics Controls tab when you set up the BenchCel Microplate Handler.

Figure

BenchCel Diagnostics Controls tab

BenchCel Microplate Handler User Guide

2 Introduction

Software description

• Diagnose problems. You can use the Jog/Teach tab on the Diagnostics Controls

page to move and adjust individual hardware components. These controls are useful for diagnosing and troubleshooting problems.

Figure

BenchCel Diagnostics Jog/Teach tab

While testing new or troubleshooting labware definitions, you can change parameters to refine the labware definition. Diagnostics includes a Labware tab on the Controls page, which enables you to adjust the labware definitions. Alternatively, you can use the Labware Editor to update the labware definitions.

Figure

BenchCel Diagnostics Labware tab

BenchCel Microplate Handler User Guide

2 Introduction

Software description

• Change general device settings. After diagnosing problems, you can change some

of the device settings to repair problems or to optimize operation.

Related information

For more information about... See...

Creating and running protocols in VWorks

Figure

BenchCel Diagnostics General Settings tab

VWorks Automation Control User Guide

VWorks setup instructions VWorks Automation Control Setup Guide

Setting up profiles and teachpoints “Setup workflow” on page 64

Using Diagnostics to troubleshoot problems

BenchCel ActiveX control BenchCel ActiveX User Guide

“Diagnostic tools” on page 146

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

This chapter contains the following topics:

• “Installation workflow” on page 36

• “Verifying laboratory requirements” on page 37

• “Unpacking BenchCel Microplate Handler” on page 42

• “Mounting the robot” on page 44

• “About integrating devices in a BenchCel Workstation” on page 47

• “Connecting the power source” on page 50

• “Connecting the pendant directly to the BenchCel device” on page 51

• “Connecting the pendant through the Robot Disable Hub” on page 53

• “Connecting and disconnecting the air source” on page 55

• “Connecting the computer” on page 57

• “Installing the shield” on page 61

3 Installing BenchCel Microplate Handler

WARNING

Installation workflow

About this topic.

This topic presents the sequence of installation procedures for the BenchCel Microplate Handler. Typically, an integrated BenchCel Workstation will be installed for you.

Changing the configuration or components of the installed workstation might invalidate the safety compliance and lead to personal injury or equipment damage.

Workflow

Step For this task… See…

1 Verify that the installation

location meets the site requirements.

2 Unpack the computer and

connect the monitor, power, mouse, and keyboard.

3 Install the VWorks

software.

4 Unpack the BenchCel

device.

5 Install the BenchCel risers,

if applicable.

6 Mount the robot head on

the BenchCel device.

7 Integrate external devices. “About integrating devices in a BenchCel

8 Connect the BenchCel

Microplate Handler:

Power

“Verifying laboratory requirements” on page 37

Manufacturer’s instructions

• VWorks Automation Control Installation

Guide (VWorks 14.0 and later)

• Software release notes (VWorks 13.1.x)

“Unpacking BenchCel Microplate Handler” on page 42

Agilent Technical Support

“Mounting the robot” on page 44

Workstation” on page 47

“Connecting the power source” on page 50

Pendant • “Connecting the pendant directly to the

BenchCel device” on page 51

• “Connecting the pendant through the

Robot Disable Hub” on page 53

Air supply “Connecting and disconnecting the air

source” on page 55

Computer “Connecting the computer” on page 57

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Verifying laboratory requirements

Step For this task… See…

9 Install the safety shield. “Installing the shield” on page 61

10 Install the labware racks. “Installing and uninstalling the labware

racks” on page 123

11 Install the integrated

devices.

12 Set up the BenchCel device

profile and set the teachpoints.

Related information

For information about… See…

Installing external devices External device user documentation

Setting up the BenchCel Microplate Handler

Defining labware and writing protocols VWorks Automation Control Setup Guide

Verifying laboratory requirements

Installation guide for the device.

“Setup workflow” on page 64

“Setting up the BenchCel device” on page 63

Laboratory space

Laboratory table or bench requirements

Make sure the table for the BenchCel Microplate Handler has the following:

• Proximity to power and air sources

• Enough space to accommodate the complete configuration of the BenchCel

Microplate Handler, computer, monitor, pendant, and integrated devices.

• Enough strength to support the weight of the BenchCel Microplate Handler and

integrated devices without excessive shaking or movement.

• Be fixed in place, for example, casters that lock.

• Sufficient clearance on the back side of the BenchCel Microplate Handler to access

power, communication, and air tubing connections and for maintenance tasks.

• Proper height for any operator to comfortably operate the BenchCel Microplate

Handler

The table must be level in the direction of the width and the depth of the platform. Using a traditional bubble level, the table should be leveled such that the bubble is centered between the two limit lines of the level.

The table surface must have a thickness relative to the material that will prevent warping when the BenchCel Microplate Handler and computer are set upon the table.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Verifying laboratory requirements

The table surface must be attached to the table frame.

The table frame must have:

• A leveling mechanism in the feet or casters.

• Dimensions that enable support of the table surface without overhang.

Space requirements

The minimum space requirements for your BenchCel Microplate Handler depends on its configuration and labware rack size. The following table lists dimensions for a BenchCel Microplate Handler in a two-, four-, or six-stack configuration.

Dimension Two stacks Four stacks Six stacks

Height, device without Risers 46.3 cm 46.3 cm 46.3 cm

Height, device with Risers (146-mm) 60.8 cm 60.8 cm 60.8 cm

Labware rack height* (250-mm, 660-mm, 860-mm)

Width 43.2 cm 86.4 cm 129.5 cm

Depth 20.3 cm 20.3 cm 20.3 cm

Weight, approximate**

without rack with rack (660 mm, front-loading)

*Approximately 30-mm of the rack height sits down inside the BenchCel stacker head. The overall height of a BenchCel device on risers with racks installed is as follows:

82.8 cm (250-mm rack)

126.0 cm (660-mm rack) 1438 cm (860-mm rack)

See the following figure.

**The rack weight is for the front-loading style rack and does not include liquid-filled microplates. The weight differs slightly between the top-loading and front-loading rack types.

———

28 kg

32.5 kg

39.5 kg

48.6 kg

51 kg

64.6 kg

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

250 mm

828 mm

1438 mm

1260 mm

660 mm

860 mm

Verifying laboratory requirements

Figure Example of BenchCel on risers with six stacks showing height requirements of 250-mm, 660-mm, and 860-mm racks

Addition of devices

If you are integrating an Agilent device or third-party device with your BenchCel Microplate Handler, make sure you include adequate space to accommodate these devices. See the device user documentation for space requirement information.

Electrical requirements

The BenchCel Microplate Handler has the following power requirements. For power requirements of other devices in an integrated workstation, see the applicable device user documentation.

Utility Requirement

Electrical 100–240 V~, 50/60 Hz

Operating AC current 5 A at 120 V~, 2.5 A at 240 V~

Fuse

Compressed air requirements

The BenchCel Microplate Handler requires the use of clean, dry, compressed air to move pneumatic components inside the device. The compressed air can be from the following sources:

• Centralized source (house)

• Compressed-air cylinders

• Portable pumps

5 A, 250 V, 5

× 20 mm, fast acting

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

CAUTION

Verifying laboratory requirements

Using oil compressors can cause oil to leak into the BenchCel Microplate Handler and void your warranty.

Air pressure greater than 0.69 MPa (100 psi) can damage the BenchCel Microplate Handler.

To maintain the desired air supply in the device, the BenchCel Microplate Handler requires a source of air as follows:

Requirement Value

Quality Clean, dry, compressed

Flow rate 34.0 Lpm (1.2 cfm)

Pressure 0.65–0.69 MPa (95–100 psi)

Environmental requirements

The lab must meet the following environmental requirements.

Requirement Value

Ambient temperature

Humidity condition

Elevation 1–2000 m

Make sure the BenchCel Microplate Handler is located away from the following:

• Heat and air conditioning ducts.

• Direct sunlight.

Computer requirements

The requirements of the controlling computer depend on the lab automation software you are using.

• VWorks 14.0 or later, see the installation guide for the software.

• VWorks 13.1.x, see the software release notes.

For third-party automation software, see the user documentation supplied with the product.

5–40

°C

10–90%

RH, non-condensing

Networking considerations

The supplied computer comes with a serial port and two Ethernet ports. You can connect the computer to the BenchCel Microplate Handler using either the serial port or one of the Ethernet ports. You can use the second Ethernet port to connect the

BenchCel Microplate Handler User Guide

computer to your local area network (LAN). You must provide an Ethernet cable for the

WARNING

LAN connection and make sure the lab has the proper network hookups for the connection.

If you are supplying your own computer, consider whether you will:

• Connect the computer to the BenchCel Microplate Handler using a serial or

• Connect the computer to your LAN.

Connecting the BenchCel Microplate Handler to a company or general network can potentially cause injury. Remote computer operators might accidentally initiate an operation that causes the robot to move unexpectedly, possibly injuring nearby lab personnel.

If you plan to connect the computer to the BenchCel Microplate Handler and to your company’s LAN, the computer might require two Ethernet cards. Two Ethernet cards allow the BenchCel Microplate Handler to operate on an isolated network.

Related information

3 Installing BenchCel Microplate Handler

Verifying laboratory requirements

Ethernet connection.

For information about… See…

Lab requirements for external devices External device user documentation

Contacting technical support “Reporting problems” on page 162

Installing the BenchCel Microplate Handler

“Installation workflow” on page 36

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

WARNING

Unpacking BenchCel Microplate Handler

About this topic

This topic describes how to unpack the BenchCel Microplate Handler from the shipping containers.

Shipping containers

The shipping containers include:

• BenchCel crate containing the BenchCel device.

• Peripherals box containing the following packages: – BenchCel utility kit – Pendant – BenchCel robot head – Safety shield with hardware – Labware racks

• Computer box

Depending on the configuration ordered, additional packages or items can be included.

Before you start

Verify the following:

1 BenchCel utility kit contents. At a minimum, the kit contains the following:

• Power cord

• Serial and Ethernet cables, and Ethernet switch

• Tubing for air line

• Software CD-ROM

2 Site specifications. Ensure the installation site meets the requirements. See

“Verifying laboratory requirements” on page 37.

Note the dimensions of the shipping container before moving it to make sure you have adequate clearance through doorways and passages.

3 Tools and equipment requirements. Obtain the following:

• Large screwdriver to open the shipping crate

• Hex wrenches: 5-mm and 2-mm for mounting the robot head

• Cart for moving the BenchCel device

4 Personnel requirements. Make sure two people are available to lift the BenchCel

device from the crate.

BenchCel Microplate Handler User Guide

Procedure

CAUTION

The packing materials and shipping container were designed to protect the device. Packing the BenchCel device using other materials might damage the device and void your warranty. Save the packing materials and shipping container in case you are required to move or ship the BenchCel device.

To unpack the BenchCel Microplate Handler:

1 Use a large screwdriver to open the BenchCel device shipping container. 2 Lift the contents out of the container and set each item carefully on the lab bench

or final location where you want to install the device.

3 Remove the packing foam from the device. 4 Remove components from the plastic bags or other packing material. 5 Inspect the unpacked items.

About installing risers

The 146-mm tall risers can be installed to the underside of the BenchCel device at each end to lift the device above the table surface. The risers are required for integrating the BenchCel device with certain devices in a workstation. In you are integrating a BenchCel Microplate Handler on risers, the Agilent installer will install the BenchCel risers before mounting the robot head on the BenchCel device.

3 Installing BenchCel Microplate Handler

Unpacking BenchCel Microplate Handler

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

CAUTION

Mounting the robot

About this topic

Typically, the BenchCel Microplate Handler will be installed for you. However, this topic provides a reference for how to mount the robot on the BenchCel device.

Procedure

Do not use the robot arms to lift the robot. Doing so could damage the arms. Ensure that you lift the robot by the base and the head. Dropping or bumping the robot can damage the electronics. Handle the robot carefully.

Figure Correct and incorrect lift points on the robot head

To mount the robot on the BenchCel device:

1 Remove the shipping bracket from the BenchCel x-axis carriage as follows:

a Refer to the following figure to locate the BenchCel x-axis carriage. b Loosen the two large black bracket screws that prevent the x-axis carriage

from moving during shipping.

c Use a 5-mm hex wrench to remove the four screws that secure the shipping

bracket to the x-axis carriage.

d Carefully lift the shipping bracket straight up, ensuring that you do not scratch

the electronics.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Stacker head

x-axis carriage

x-axis carriage (closeup view)

Alignment pins

Mounting the robot

Figure

BenchCel device with shipping bracket removed from the x-axis carriage

2 Slide the x-axis carriage into position directly under a stacker head opening. 3 Inspect the x-axis carriage and ensure any cables are recessed. 4 Using the correct lift points, grasp the robot head and, carefully, lower it onto the

x-axis carriage, aligning the two holes on the robot z-column bottom with the dowel pins on the x-axis carriage.

5 Gently slide the robot downwards. When the robot is in position, push down on it to

ensure the electronics are fully connected.

6 Gently rotate the robot arms to one side to expose the two screw holes on the

robot base. Insert the two 35-mm M6 screws and tighten to secure the robot to the base.

Figure

BenchCel robot with rotated arms to expose screw holes in the base

7 Lift the robot head from the base to expose the remaining two screw holes in the

base. While firmly supporting the robot head, install the two 45-mm M6 screws to finish securing the robot base to the x-axis carriage.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

WARNING

Mounting the robot

Figure

The robot head may not remain in the up position. The robot head can drop, potentially pinching your fingers. Ensure that you support the weight of the robot head while it is in the up position.

BenchCel robot head in up position: (1) mounting screw holes and (2) front panel

8 Gently lower the robot head onto the base. 9 To secure the front panel to the robot base, use a 2-mm hex wrench to install the

Related information

For more information about… See…

Installation requirements “Verifying laboratory requirements” on

Installing the BenchCel Microplate Handler

two screws.

page 37

“Installation workflow” on page 36

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

WARNING

IMPORTANT

About integrating devices in a BenchCel Workstation

About this topic

Typically, the BenchCel Microplate Handler, any supplied devices, and the safety equipment in a workstation will be installed for you.

This topic provides basic integration concepts: how integration plates are used and how to adjust device positions. The workstation layout and the device integration procedures can vary, depending on the combination of devices you are integrating and the desired configuration.

Before you start

Changing the configuration or components of the installed workstation might invalidate the safety compliance and lead to personal injury or equipment damage.

Contact Agilent Technical Support if you want to install the BenchCel device on risers or integrate a new device.

Make sure you have the following:

• The external devices you want to integrate

• Two integration plates for the BenchCel device

• Integration plate for each external device

• 4-mm hex wrench

• 3-mm hex wrench

Installing the BenchCel device integration plate

The BenchCel device integration plates have locking mechanisms that keep the BenchCel device and any external device in position during a run. The integration plates must be installed under the BenchCel device.

To install the BenchCel device integration plate:

1 Position the integration plates on the benchtop. 2 If risers are required in the integration, install the risers on the integration plates. 3 Place the BenchCel device on the integration plates or risers. 4 Tighten the screws to secure the device on the integration plates.

Installing the external device integration plate

BenchCel Microplate Handler User Guide

You can integrate an external device on the left or right side of the BenchCel device. For each external device, you must install the integration plate that is specific to that device.

3 Installing BenchCel Microplate Handler

Clamp screw

Integration plate BenchCel device

About integrating devices in a BenchCel Workstation

To install the external device and integration plate:

1 Depending on whether you are integrating a device to the left or right of the

BenchCel device, loosen the left or right clamp screw using the 3-mm hex wrench. See the following figure.

As you loosen the screw, the left or right clamp opens.

Figure

Clamp screw (left side) on BenchCel device

2 Mount the external device on its integration plate. Tighten the bolts or screws to

secure the device on the integration plate.

3 Place the external device and integration plate next to the BenchCel device.

Figure

External device and integration plate next to the BenchCel device

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Tighten slightly so that the plate can still move.

About integrating devices in a BenchCel Workstation

4 Use the 3-mm hex wrench to slightly tighten the BenchCel device integration plate

clamp screw. You will not tighten the screw all the way until the devices are correctly aligned.

Figure

Integration plate clamp screw

5 Visually inspect the position of the integrated device relative to the BenchCel

device. The two devices should be aligned along the y-axis (front-to-back direction) so that the BenchCel robot can place a microplate on the plate stage accurately. If the devices are not properly aligned, slide the devices along the y-axis until they appear to be correctly aligned.

Note: You will verify and refine the alignment when you set up the teachpoints for the external devices.

6 Repeat the procedure for each external device.

Related information

For more information about… See…

Integration options “Integration options for workstations” on

BenchCel Microplate Handler installation requirements

External device installation requirements

Installing the BenchCel Microplate Handler

Setting up the BenchCel Microplate Handler

BenchCel Microplate Handler User Guide

page 25

“Verifying laboratory requirements” on page 37

External device user documentation

“Installation workflow” on page 36

“Setup workflow” on page 64

3 Installing BenchCel Microplate Handler

WARNING

Connecting the power source

About this topic

This topic explains how to connect the BenchCel Microplate Handler to a grounded power source.

Before you start

Make sure you have the supplied power cord.

Ensure that the power cords are in good condition and are not frayed. Use of frayed or damaged power cords can cause injury. Use of incorrect power cords can cause damage to the device.

Connecting the power source

To connect the BenchCel device to a grounded power source:

1 Plug one end of the power cord into the AC power entry located on the back of the

BenchCel Microplate Handler.

2 Plug the other end of the cord into an AC outlet with grounded circuit.

Related information

For more information about… See…

Figure

Connectors on the back of the BenchCel Microplate Handler

Electrical requirements “Verifying laboratory requirements” on

page 37

Installing the BenchCel Microplate Handler

“Installation workflow” on page 36

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

IMPORTANT

Connecting the pendant directly to the BenchCel device

For more information about… See…

Setting up the BenchCel Microplate Handler

Operating the BenchCel Microplate Handler

“Setup workflow” on page 64

“Workflow for operating the BenchCel Microplate Handler” on page 116

Connecting the pendant directly to the BenchCel device

About this topic

If you are integrating the BenchCel Microplate Handler with devices that are not equipped with safety interlock circuitry, for example, the PlateLoc Sealer or the Microplate Labeler, use the procedure in this topic to connect the emergency-stop pendant.

Figure

Emergency-stop pendant

The pendant must be connected to close the safety interlock circuit. The circuit must be closed for the device to operate.

If the BenchCel Microplate Handler is integrated with devices that are equipped with safety interlock circuitry, such as the Bravo Platform, you connect the pendant through a main emergency-stop control, such as the Robot Disable Hub. See “Connecting the

pendant through the Robot Disable Hub” on page 53.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Connecting the pendant directly to the BenchCel device

Procedure

To connect the pendant directly to the BenchCel device:

Connect the pendant cable to the pendant port on the back of the BenchCel device.

Related information

For more information about… See…

Stopping in an emergency “Emergency stops” on page 5

Safety interlock circuit “Safety interlock circuit” on page 9

Installing the BenchCel Microplate Handler

“Installation workflow” on page 36

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Connecting the pendant through the Robot Disable Hub

About this topic

Read this topic if you are integrating the BenchCel Microplate Handler with other devices that are equipped with safety interlock circuitry, such as the Bravo Platform. This topic describes how to connect the emergency-stop pendants for the integrated devices through the Robot Disable Hub, which provides a main emergency-stop control for the workstation.

Figure

Figure Robot Disable Hub, 3-Port

Emergency-stop pendant

Robot Disable Hub overview

The Robot Disable Hub can connect the interlock circuits of up to three devices and a light curtain. Pressing the red button on a pendant connected through the Robot Disable Hub will trip the interlock circuit, causing all the devices on the circuit to stop.

The Robot Disable Hub has the following connectors, controls, and indicators:

Figure

• Pendants connectors accommodate the BenchCel pendant and up to two

• Devices connectors accommodate the BenchCel device and up to two other

• Light Curtain connectors connect to the corresponding light curtain receiver (Rx)

• Power status indicator lights green when the unit is connected to power.

BenchCel Microplate Handler User Guide

Robot Disable Hub connections (front and back views)

additional pendants.

devices with interlock circuits.

and transceiver (Tx) cables.

3 Installing BenchCel Microplate Handler

IMPORTANT

Devices

Pendants Power

Light Curtain

00220 Bravo Back

00220c

6 7

Connecting the pendant through the Robot Disable Hub

• Power entry port connects to the supplied 24W power adapter, which in turn

connects to the power cord.

• Light Curtain Enable/Bypass switch enables or disables the light curtain. You set the

switch to Bypass during the installation. After installing the light curtain and connecting all the devices, you set the switch to Enable.

Any unused Pendants connectors require E-stop jumpers to close the interlock circuit.

Example of connections using Robot Disable Hub

The following figure and table provide an example of connecting the BenchCel device and the Bravo Platform through the Robot Disable Hub.

Item Description Connects from Connects to Robot

1 Extension cable Bravo pendant

port

Disable Hub

Devices connector

2 Pendant cable Pendant 1 Pendants connector

3 Pendant cable Pendant 2 Pendants connector

4 Extension cable BenchCel pendant

port

Devices connector

5 Jumper Jumper Pendants connector

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

WARNING

Connecting and disconnecting the air source

Item Description Connects from Connects to Robot

6,7Light curtain transmitter and

receiver cables

(not shown)

Bravo Light Curtain

Related information

For more information about… See…

Stopping in an emergency “Emergency stops” on page 5

Safety interlock circuit “Safety interlock circuit” on page 9

Installing the BenchCel Microplate Handler

“Installation workflow” on page 36

Connecting and disconnecting the air source

Disable Hub

Light Curtain connectors

About this topic

Compressed air is used to move parts inside the BenchCel Microplate Handler. This topic explains how to connect the BenchCel Microplate Handler to the air source and check the connections for leaks before use.

Before you start

Make sure you have the supplied air tubing.

Working with open, charged air lines can result in injury. Turn off the compressed air line when disconnecting or reconnecting devices that use compressed air. Contact your facilities department or Agilent Technical Support with questions about setting up the air line.

Connecting the air source

To connect the BenchCel device to the air source:

1 Turn off the air at the source (house, cylinder, or pump). 2 Connect one end of the air tubing to the air source (house, cylinder, or pump), and

then connect the free end of the tubing to the quick disconnect fitting at the airinput port.

To connect the tubing, push the end of the tubing into the quick disconnect fitting at the air source and on the back of the BenchCel Microplate Handler.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

CAUTION

Connecting and disconnecting the air source

Figure

Air tubing connection on the back of the BenchCel Microplate Handler

Checking the air connections

To check the air connections:

1 With the air source turned off, gently tug the air tubing at each connection.

If you feel resistance at the connection, the tubing has been properly installed.

2 Turn on the air at the source (house, cylinder, or pump). 3 Listen near each connection for hissing sounds that might indicate a leak.

If you hear hissing sounds, turn off the air at the source, check and tighten the connections, and then turn on the air again. If the problem persists, contact your facilities department or Agilent Technical Support.

To air source (house, cylinder, or pump)

Disconnecting the air source

Before moving or shipping the BenchCel Microplate Handler and before performing maintenance or cleaning, disconnect the air tubing.

Do not pull the tubing out of the orange quick-disconnect fitting. Doing so can damage the fitting.

To disconnect the air tubing from the BenchCel device

1 Turn off the air at the source (house, cylinder, or pump). 2 Push and hold the locking collar against the fitting, and then gently pull the air

tubing out. Note: Alternatively, you can use the SMC Pneumatics tool (TG-2) to aid in this

task. See the manufacturer’s documentation for use instructions. Contact your local SMC parts supplier for ordering details.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Push and hold the locking collar.

Gently pull out the tubing.

Connecting the computer

Related information

For more information about... See...

Air-source requirements “Verifying laboratory requirements” on

How to set up the BenchCel Microplate Handler

How to operate the BenchCel Microplate Handler

Figure

Quick-disconnect fitting (close-up view)

page 37

“Setup workflow” on page 64

“Workflow for operating the BenchCel Microplate Handler” on page 116

Connecting the computer

About this topic

This topic explains how to connect the controlling computer to the BenchCel Microplate Handler.

Before you start

Make sure you have the following:

• Ethernet cable (supplied) or RS-232 DB9 serial cable

• Ethernet switch (supplied) for Ethernet connections only

Connecting the controlling computer

You can connect the computer to the BenchCel Microplate Handler in one of the following ways:

• Serial connection. You can use the supplied RS-232 DB9 serial cable to connect the

computer to the BenchCel Microplate Handler.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Connecting the computer

• Ethernet connection. You can use the supplied Ethernet cable to connect the

computer to the BenchCel Microplate Handler. The Agilent configured computer has two Ethernet ports. You can use one port to connect to the BenchCel Microplate Handler and the other to connect to your company network.

To connect the controlling computer to the BenchCel device:

1 Turn off the computer. 2 Serial connection only. Do the following:

a Connect the female end of the serial cable to a COM port on the controlling

computer. Note the number of the COM port. You will provide this number in the software.

b Connect the male end of the serial cable to the serial port on the back of the

BenchCel Microplate Handler.

Figure

Serial connector on the BenchCel Microplate Handler (back view)

3 Ethernet connection only. Do the following:

a Connect one end of the Ethernet cable to the back of the BenchCel device. b Connect the free end of the Ethernet cable to any available port on the Ethernet

switch.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

WARNING

Connecting the computer

Figure

Ethernet connector on the BenchCel Microplate Handler (back view)

Configuring the computer network card (Ethernet connection only)

The computer configured by Agilent Technologies is already set up to communicate with the BenchCel Microplate Handler. No change to the network card IP address is required.

If you are using a computer that was not configured by Agilent Technologies, make sure the value of the network card IP address and subnet mask are as follows:

• IP address: 192.168.0.1

• Subnet mask: 255.255.255.0

If your computer will be connected to your LAN, make sure the computer has a second network card. The second network card can have a dynamic IP address.

Connecting the computer to external devices (Ethernet connection only)

To connect the external devices to the controlling computer:

1 Connect one end of the Ethernet cable to the Ethernet port on the external device.

See the device user documentation for details.

2 Connect the free end of the Ethernet cable to any available port on the Ethernet

switch.

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Connecting the computer

Related information

For more information about… See…

The controlling computer requirements “Verifying laboratory requirements” on

The Ethernet switch The Ethernet switch user documentation

IP addresses The Microsoft Windows user

page 37

documentation

Installing the BenchCel Microplate Handler

Setting up the BenchCel Microplate Handler

Operating the BenchCel Microplate Handler

“Installation workflow” on page 36

“Setup workflow” on page 64

“Workflow for operating the BenchCel Microplate Handler” on page 116

BenchCel Microplate Handler User Guide

Installing the shield

WARNING

About this topic

This topic explains how to install the safety shield on the front of the BenchCel Microplate Handler. Additional shields may be required depending on the workstation configuration. Typically, the BenchCel Microplate Handler and workstation safety equipment will be installed for you.

Changing the configuration or components of the installed workstation might invalidate the safety compliance and lead to personal injury or equipment damage.

Before you start

Make sure you have the following:

• 3-mm hex wrench

• Low-head M5 screws (supplied)

• 1 shield for each pair of stackers

For example, a BenchCel Microplate Handler with six stackers requires three shields, as the following figure shows.

3 Installing BenchCel Microplate Handler

Installing the shield

BenchCel Microplate Handler User Guide

3 Installing BenchCel Microplate Handler

Top view - back

Insert screws

Installing the shield

Procedure

To install the safety shield:

1 Place the safety shield on the front of the BenchCel Microplate Handler centered

between the pair of stackers. The ledge of the shield should sit on the top of the device.

2 Insert the four M5 screws into the shield.

Figure

Safety shield on BenchCel Microplate Handler with two stackers (top view)

3 Tighten the screws using the M3 hex wrench. 4 Repeat steps 1 to 3 for each pair of stackers on the BenchCel Microplate Handler.

Related information

For more information about… See…

Safety shield description “Hardware overview” on page 15

Safety information “Safety guidelines” on page 1

Installing the BenchCel Microplate Handler

Setting up the BenchCel Microplate Handler

Operating the BenchCel Microplate Handler

“Installation workflow” on page 36

“Setup workflow” on page 64

“Workflow for operating the BenchCel Microplate Handler” on page 116

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

This chapter explains how to set up the BenchCel Microplate Handler for operation. This chapter contains the following topics:

• “Setup workflow” on page 64

• “Starting up and shutting down” on page 66

• “Creating BenchCel device in the VWorks software” on page 68

• “Opening BenchCel Diagnostics” on page 73

• “Creating profiles” on page 76

• “Initializing the device” on page 82

• “Setting and managing teachpoints” on page 84

4 Setting up the BenchCel device

Setup workflow

About this topic

This topic presents the workflow for setting up the BenchCel Microplate Handler for operation.

Before you start

Before setting up the BenchCel Microplate Handler, you should already have definitions for the labware you want to use. For instructions on how to define labware in the VWorks software, see the VWorks Automation Control Setup Guide.

Before setting teachpoints (step 7 in the setup workflow), you should also adjust the BenchCel sensor thresholds for each type of labware that you use. For details, see

“Setting sensor thresholds” on page 99.

Workflow

The following table presents the steps for setting up the BenchCel Microplate Handler. After setting up the BenchCel Microplate Handler for the first time, you will not likely change any of the settings in the procedure unless you add a device, replace a device, or move the BenchCel Microplate Handler.

BenchCel Microplate Handler User Guide

Step For this task… See…

Set and edit teachpoints

for external devices.

Write protocols.

Create a profile for

each external device.

Create a profile.

Create a device file and add the

BenchCel device in VWorks.

Add external devices

to the device file.

Start up the workstation.

Set the sensor thresholds

for your labware.

4 Setting up the BenchCel device

Setup workflow

“Starting up and shutting down” on page 66

2 “Creating BenchCel device in the VWorks software” on

page 68

3 “Creating profiles” on page 76

4 User documentation for the devices

5 User documentation for the devices

6 “Setting sensor thresholds” on page 99

7 “Setting and managing teachpoints” on page 84

8 VWorks Automation Control User Guide

Related information

BenchCel Microplate Handler User Guide

For more information about… See…

Installation procedures “Installation workflow” on page 36

Operating the BenchCel Microplate Handler

“Workflow for operating the BenchCel Microplate Handler” on page 116

Troubleshooting problems “Maintenance and troubleshooting” on

page 129

4 Setting up the BenchCel device

WARNING

IMPORTANT

Starting up and shutting down

About this topic

This topic explains how to start up and shutdown the BenchCel Microplate Handler.

Starting up the device

To avoid potential injury do not touch the BenchCel Microplate Handler as you start the software. The robot head moves when the device initializes.

The BenchCel safety interlock circuit must be closed for the device to operate.

To start up the BenchCel Microplate Handler:

1 On the back of the BenchCel Microplate Handler, press the power switch to the on

(I) position.

Figure

Every time you turn on the BenchCel Microplate Handler, the robot homes (the robot is sent to the factory-defined home position for each axis of motion). If the BenchCel robot does not home, make sure power to the robot has been restored. (See “Recovering from an emergency stop” on page 6 to reset the emergency-stop button.) If the button has been reset and the robot still does not home, turn off the device, check the connections, and turn it on again.

Power switch on the BenchCel Microplate Handler (back)

2 Turn on all other devices integrated with the BenchCel Microplate Handler. See the

device user documentation for instructions.

3 Turn on the compressed air supply to the BenchCel Microplate Handler.

BenchCel Microplate Handler User Guide

4 Turn on the controlling computer. See the user documentation from the computer

manufacturer.

5 To start the VWorks software, do one of the following:

• Double-click the shortcut icon on the Windows desktop

• Go to the Windows start menu () > All Apps > Agilent Technologies >

VWorks.

Shutting down the BenchCel Microplate Handler

Shut down the BenchCel Microplate Handler if you intend to:

• Leave it unused for a long period of time.

• Service the device.

• Move it to another location.

To shut down the BenchCel Microplate Handler:

1 Follow the post-run clean-up procedure after the last protocol run. See “Cleaning up

after a protocol run” on page 132.

2 Exit the VWorks software. 3 If you use devices that require a vacuum pump, turn off power at the pump if the

pump module has an on/off switch.

4 Turn off the compressed air to the BenchCel Microplate Handler and other devices. 5 Turn off the BenchCel Microplate Handler. To do this, on the back of the device,

press the power switch to the off (O) position.

4 Setting up the BenchCel device

Starting up and shutting down

Figure

Power switch on the BenchCel Microplate Handler (back)

6 Turn off other devices in the BenchCel Microplate Handler.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Creating BenchCel device in the VWorks software

Related information

For information about… See…

Setting up the BenchCel Microplate Handler

Cleaning up after a protocol run “Cleaning up after a protocol run” on

Troubleshooting startup or shutdown problems

“Setup workflow” on page 64

page 132

“Maintenance and troubleshooting” on page 129

Creating BenchCel device in the VWorks software

About this topic

Read this topic if you are an administrator responsible for managing Agilent devices that are controlled by the VWorks software. This topic describes how to add and delete new BenchCel devices in the VWorks software.

The VWorks software uses the information in a device file to communicate with and operate devices within the lab automation system.

• If your computer was configured by Agilent Technologies. The correct device

configuration is already set up for communication with the BenchCel Microplate Handler. You are not required to create a new BenchCel device in the software unless you want to reference different profiles, integrate additional devices with the workstation, or change the rack size. To establish communication, you must initialize the device.

• If you configured your own computer. You must add a device in the VWorks

software for the BenchCel Microplate Handler. You must also add devices in the software for any devices that are physically integrated with the workstation.

For detailed information about device files and associations with profiles, teachpoints, and labware definitions, see the VWorks Automation Control User Guide. For instructions on how to add third-party devices, see the Device Driver User Guide.

Devices and device files defined

A device is an item in your lab automation system that has an entry in a VWorks device file. A device can be a robot, an instrument, or a location in a lab automation system that can hold a piece of labware.

The device file (*.dev) stores information for all the devices in an integrated system, including:

• Type of device (for example, BenchCel device)

• Device configuration information (for example, approach height, allowed or

prohibited labware, and so on)

• Profile to use

BenchCel Microplate Handler User Guide

Creating a device file

If you are setting up the BenchCel Microplate Handler for the first time, you will create a device file. You add the BenchCel device and the external devices to the device file.

To create a device file:

1 Log in to the VWorks software as an Administrator. 2 In the VWorks window, choose File > New > Device.

3 Choose File > Save. In the Save File As dialog box, type a file name (*.dev), select the

4 Setting up the BenchCel device

Creating BenchCel device in the VWorks software

A Device File tab appears in the VWorks window.

storage location, and click Save:

4 VWorks Plus only. If an audit trail is being logged for records in the development

state, the Audit Comment dialog box opens. Select or type the audit comment, and then click OK.

Adding the device to a device file

Before you begin:

• Ensure that any devices are physically networked to the computer.

• Turn on the devices.

To add devices to a device file:

1 In the VWorks window, verify that the correct device file is open.

To open a device file, choose File > Open > Device. In the Open File dialog box, locate and select your device file (*.dev), and then click Open.

2 In the Available Devices area, double-click the BenchCel icon. Or, drag the BenchCel

icon to the Device File tab.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

IMPORTANT

Creating BenchCel device in the VWorks software

Note: To show or hide the list of available devices, choose View > Available Devices.

3 In the Device File tab, select the BenchCel-n icon. 4 Under BenchCel Properties, type a Name for the device. By default, the software

assigns BenchCel-n, and increments the number for each BenchCel device that you add.

To identify the specific BenchCel, the device name should include the device serial number.

5 In the Profile list, select a profile for the device.

If the Profile list is empty, open BenchCel Diagnostics and create a profile. Then return to the Profile list under BenchCel Properties and select the new profile.

To use different configurations of the same device in different protocols, you can save time by creating a different device file for each configuration. For example, if you change a pipette head on a pipettor, you can simply open the device file that contains the device with the appropriate profile instead of editing the profile selection in the device file.

6 On the Device File tab, expand the BenchCel device icon to show the list of stackers,

and then click the Stacker 1 icon. The corresponding stacker properties appear.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

IMPORTANT

Creating BenchCel device in the VWorks software

Set the desired values for the following properties. Use the default values for the remaining properties.

Property Description

Allowed/prohibited labware

Optional. Click if you want to specify labware restrictions for this location. The Allowed/prohibited labware dialog box appears. For details on the labware classes, see the VWorks Automation Control Setup Guide.

Note: If the button is not visible, click the empty field.

Stack Height Type the maximum height (mm) of the rack you are

using at this location. For example, type 660 if you are using a 660-mm tall rack.

Do not change the default value of the Teachpoint for robot BenchCel property. For example, the stacker 1 teachpoint identity is 1, the identity of stacker 2 is 2, and so forth.

Repeat this step for each stacker.

7 Select File > Save.

If you are creating a new device file, the Save File As dialog box appears so that you can specify a name and location for your device file (*.dev).

Alternatively, you can select File > Save All to save the device file and the current protocol file at the same time.

8 VWorks Plus only. If an audit trail is being logged, the Audit Comment dialog box

opens. Select or type the audit comment, and then click OK.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Creating BenchCel device in the VWorks software

Specifying BenchCel accessibility at other devices

For any device in the workstation that the BenchCel robot will access, use the following procedure to set the Teachpoint for robot Agilent BenchCel property in the device file after you set the BenchCel teachpoint at the given device.

To set the Teachpoint for robot Agilent BenchCel property at a device:

1 In the VWorks window, open the device file that contains the BenchCel robot. 2 In the Devices area, click the location in the device that the BenchCel robot will

access. For example, if the device file contains the BenchCel and Bravo devices, click the

Bravo deck location that the BenchCel robot will access.

3 In the Location Properties for the selected location, click the setting for the

Teachpoint for robot Agilent BenchCel property.

In the following example, the BenchCel robot will transfer labware to and from Bravo deck location 3. In the Bravo Location Properties for deck location 3, the Bravo 3 teachpoint is selected. The teachpoint name, Bravo 3, is assigned when this teachpoint is set in BenchCel Diagnostics.

4 Select File > Save to save the change to the device file. 5 VWorks Plus only. If an audit trail is being logged, the Audit Comment dialog box

opens. Select or type the audit comment, and then click OK.

BenchCel Microplate Handler User Guide

Related information

For information about… See…

VWorks software VWorks Automation Control User Guide

4 Setting up the BenchCel device

Opening BenchCel Diagnostics

Setting up BenchCel Microplate Handler

Profiles “Creating profiles” on page 76

Teachpoints “Setting and managing teachpoints” on

Opening BenchCel Diagnostics

About this topic

To establish communication between the host computer and the device, you initialize the device. You can initialize the device in the following ways:

• Diagnostics. To control the device using the Diagnostics software.

• VWorks window. To use VWorks to control the device, for example, to run a

protocol, you initialize the devices in the VWorks window.

This topic describes what happens during initialization.

“Setup workflow” on page 64

page 84

Procedure

To open Diagnostics:

1 In the VWorks window, ensure that the correct device file is open.

To open a device file, choose File > Open > Device, and then select the appropriate device file (*.dev) in the Open File dialog box.

2 In the Devices area of the opened device file tab, highlight the device icon, and then

click Device diagnostics. Alternatively, you can double-click the device icon.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Opening BenchCel Diagnostics

The device’s diagnostics dialog box opens.

Figure BenchCel Diagnostics window with Profiles page displayed

Note: For VWorks 14.0 and later versions, the Profiles page contains log areas.

BenchCel Microplate Handler User Guide

Related information

For information about… See…

VWorks software VWorks Automation Control User Guide

4 Setting up the BenchCel device

Opening BenchCel Diagnostics

Setting up BenchCel Microplate Handler

Profiles “Creating profiles” on page 76

Teachpoints “Setting and managing teachpoints” on

BenchCel Diagnostics features “Diagnostic tools” on page 146

“Setup workflow” on page 64

page 84

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

IMPORTANT

CAUTION

Creating profiles

About this topic

The computer configured by Agilent Technologies already has the correct profile to communicate with the BenchCel Microplate Handler. A new BenchCel profile is not required unless you want to set up unique communication settings or use a different teachpoint file.

If you are using a computer other than one configured by Agilent Technologies, you must create a BenchCel profile. This topic describes how to create BenchCel profiles.

You must also create profiles for devices that are integrated with the BenchCel Microplate Handler. For instructions on how to create the profiles for other Agilent devices, see the corresponding device user documentation. For instructions on how to create profiles for a third-party device, see the applicable device driver user guide.

Profiles

Each BenchCel Microplate Handler you install requires a unique profile, which enables unique communication settings.

A profile is a collection of settings that manages how you connect to devices. The software stores each profile in a device profile file (.xml).

Note: VWorks software version 13.1.x and earlier versions use the Windows Registry to store the device profile.

A BenchCel profile does the following:

• Specifies the port used to establish communication between the BenchCel

Microplate Handler and the controlling computer.

• References a teachpoint file. For a description of teachpoint files, see “Setting and

managing teachpoints” on page 84.

• Specifies the number of stackers for the BenchCel Microplate Handler.

You use the BenchCel Diagnostics software to create and manage profiles.

Note: The profile is referenced by a device file. For information about device files, see

“Creating BenchCel device in the VWorks software” on page 68. For a detailed

description of the relationships between the device file, profile, and teachpoint file, see the VWorks Automation Control User Guide.

Creating BenchCel profiles

Each profile can be used by multiple protocols. Deleting, renaming, or changing the parameters for a profile based on one protocol can invalidate other protocols that use the same profile.

Note: Unless you login to the VWorks software as an Administrator or Technician, only the Profile list and the Initialize this profile button are available in the Profile Management area of the Profiles page.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Creating profiles

To create a BenchCel profile:

1 Open BenchCel Diagnostics. 2 On the Profiles page, click Create a new profile.

3 In the Create Profile dialog box, type a name, and click OK. Use a profile name that

identifies the specific configuration, for example, you can use the device serial number.

The change appears in the log area at the bottom of the page. For a description of this area, see “About the profile logs” on page 80.

VWorks Plus only. If an audit trail is being logged for records in the development state, the Audit Comment dialog box opens. Select or type the audit comment, and then click OK.

4 In the Settings area, select one of the following:

• Ethernet connection. Select This BenchCel is connected via ethernet, and

proceed to step 5.

• Serial connection. Select This BenchCel is connected via serial, and go to step 6.

5 Ethernet connection only. Click Find available device to select the device to

associate with the profile. In the Discovered BioNet Devices dialog box that opens:

a Select the correct Ethernet adapter for the device connection. A list of devices

appear.

b In the list of devices that appear, select the BenchCel device. If you have

multiple BenchCel devices on the network, use the MAC Address to identify the BenchCel device you want. To successfully communicate with the BenchCel device, the Status column must display New or Matched for the device.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

IMPORTANT

Creating profiles

Note: To determine the MAC address, you can turn off all other BenchCel devices so that only one appears in the Discovered BioNet Devices dialog box.

c Click OK.

6 Serial connection only.

a Select the COM port number on the controlling computer. b Select Use flow control to stop the flow of data from the computer before it

overruns the device communication buffer. This option helps to optimize communication over the serial connection.

7 If you want to create a profile for a BenchCel Microplate Handler that has a different

number of stacks than the one connected to the controlling computer, set the Number of stacks value.

During initialization, the software automatically detects the actual number of stacks on the BenchCel Microplate Handler. If this number differs from the number specified by the profile, an error message appears and gives you the option to Abort, Retry, or Ignore. If you click Ignore, the software overwrites the Number of stacks value with the actual number of stacks.

8 Make a backup copy of the teachpoint file. If the original teachpoint file becomes

lost or damaged, you can use the backup copy instead of resetting all the teachpoints and creating a new file.

a In the Teachpoint File area, click New. b In the Save the Teachpoint File dialog box (VWorks 14.0 or later) or the Save As

dialog box (VWorks 13.1.x), locate the teachpoint file for your profile.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

CAUTION

Creating profiles

Note: The default teachpoint file name is Teachpoints_<profilename>.xml, where <profilename> is the name of the profile.

c Append the teachpoint file name with the word backup, and then click Save.

Note: The Teachpoint File area displays the file storage location.

9 In the Teachpoint File area, click , and in the Select a Teachpoint File dialog

If the teachpoint file was copied from another computer, you must verify the teachpoints for the new profile before using the profile.

BenchCel Microplate Handler User Guide

VWorks Plus only. If an audit trail is being logged for records in the development state, the Audit Comment dialog box opens. Select or type the audit comment, and then click OK.

box, locate and select your original teachpoint file, and then click Open. You will edit the teachpoints in this file in another procedure.

4 Setting up the BenchCel device

CAUTION

Creating profiles

10 Click Update this profile to save the changes.

VWorks Plus only. If an audit trail is being logged, the Audit Comment dialog box opens. Select or type the audit comment, and then click OK.

11 To establish communication with the BenchCel Microplate Handler, click Initialize

this profile.

To prevent a potential hardware collision, make sure you have verified the teachpoints before using a profile.

If you are setting up the BenchCel Microplate Handler for the first time, return to

“Creating BenchCel device in the VWorks software” on page 68 and select the profile

name (step 5).

About the profile logs

VWorks 14.0 and later versions only. The Profiles page contains two log areas:

• Table of variables that have been modified since the last time the profile was

• Text box that contains a running log of updates to profiles for the current

updated. The table lists the old and new values for each setting you change. The software clears the table when you click Update this profile, and logs the information in the text box at the bottom of the page.

diagnostics session. This profile log activity is included in the VWorks Main Log. For details on the Main Log, see the VWorks Automation Control User Guide.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Creating profiles

Figure

Log area in Profiles tab

Related information

For information about See...

Adding a device in VWorks software “Creating BenchCel device in the VWorks

Setting teachpoints “Setting and managing teachpoints” on

BenchCel Microplate Handler User Guide

software” on page 68

page 84

4 Setting up the BenchCel device

Initializing the device

About this topic

To establish communication between the computer and the BenchCel Microplate Handler, you initialize the BenchCel device. You can initialize the BenchCel Microplate Handler in the following ways:

• BenchCel Diagnostics. To control the BenchCel Microplate Handler using BenchCel

Diagnostics, initialize the profile in BenchCel Diagnostics.

• VWorks window. To control the BenchCel Microplate Handler from the VWorks

window, for example, to run a protocol, initialize the device in the VWorks window.

Before you start

Ensure that you have added the BenchCel device to a VWorks device file and that you have a valid BenchCel profile.

Initializing the device in Diagnostics

To initialize a profile in BenchCel Diagnostics:

1 In the VWorks window, open the device file for the profile that you want to initialize.

To open a device file, choose File > Open > .Device. In the Open File dialog box, select the file, and then click Open.

2 Click No if the Would you like to initialize devices now? message appears. 3 In the device file tab, select the device, and then click Device diagnostics to open the

BenchCel Diagnostics dialog box.

BenchCel Microplate Handler User Guide

4 In the Profiles tab, ensure that the correct profile is selected in the Profile name list,

and then click Initialize this profile to start initializing the selected profile.

Initializing the device in the VWorks software

To initialize the device from the VWorks window:

1 In the VWorks window, open the device file.

To open a device file, choose File > Open > .Device. In the Open File dialog box, select the file, and then click Open.

2 In the Devices area, highlight the device that you want to establish communication

with, and verify that the correct profile is selected in the BenchCel Properties area.

4 Setting up the BenchCel device

Initializing the device

3 Click Initialize selected devices or Initialize all devices.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Setting and managing teachpoints

Related information

For information about See...

Adding a device in VWorks software “Creating BenchCel device in the VWorks

Creating a profile “Creating profiles” on page 76

Setting and managing teachpoints

About this topic

This topic describes teachpoints and how to set them.

Teachpoints defined

software” on page 68

A teachpoint is a set of coordinates that define where the robot can pick up or place labware. The location can be on an external device or a platepad. You set, edit, and save

teachpoints in BenchCel Diagnostics. The teachpoints are displayed as plus signs ( ) in the graphical display area in the BenchCel Diagnostics dialog box.

Note: The graphical display area also shows teachpoints ( ) at the stacks. These teachpoints are preset at the factory and should not be changed.

Figure

Example teachpoints for labware pickup and placement location at integrated devices

Teachpoint files

The teachpoints you set are saved in the XML format in a teachpoint file. The default teachpoint file name is Teachpoints_<profilename>.xml, where <profilename> is the name of the profile. The Teachpoint File area in the Profiles page displays the file path.

BenchCel Microplate Handler User Guide

You must use one teachpoint file for each BenchCel device. If you integrate a new

IMPORTANT

Verify the teachpoint.

Lock the device positions.

Determine the

teachpoint coordinates.

Record the teachpoint.

Edit the teachpoint if required.

device with the BenchCel Microplate Handler, you can add the new teachpoints to the existing file. If you have multiple BenchCel devices in a workstation, you must create a teachpoint file for each BenchCel device in the workstation.

The teachpoint file is referenced by a profile. For information about profiles, see

“Creating profiles” on page 76.

Workflow for setting teachpoints

Step For this task… See…

4 Setting up the BenchCel device

Setting and managing teachpoints

“Determining the teachpoint coordinates” on page 86

2 “Locking the device positions” on page 90

3 “Recording the teachpoint coordinates” on page 91

4 “Verifying the teachpoints” on page 96

5 “Editing existing teachpoints” on page 97

6 Set the Teachpoint for robot Agilent

BenchCel property in the device file.

“Specifying BenchCel accessibility at other devices” on page 72

Before you start

BenchCel Microplate Handler User Guide

Before you start changing teachpoints, ensure that you have a backup copy of the teachpoint file. If the original teachpoint file becomes lost or damaged, you can use the backup copy instead of resetting all the teachpoints and creating a new file.

• Verify that the labware you want to use has a corresponding labware entry in the

software. To view the labware entries, click Tools > Labware Editor in the VWorks window.

• Initialize the correct profile in BenchCel Diagnostics. For details, see “Initializing the

device” on page 82.

4 Setting up the BenchCel device

WARNING

Setting and managing teachpoints

Determining the teachpoint coordinates

To prevent potential injury, make sure no one else can issue commands at the controlling computer while you are manually determining the teachpoint coordinates.

To determine the coordinates of the pickup and placement location:

1 Place two or three spare microplates in a labware rack, and then install the rack on

the BenchCel Microplate Handler. For instructions, see “Filling and emptying the

labware racks” on page 119 and “Installing and uninstalling the labware racks” on page 123.

2 Open BenchCel Diagnostics for the BenchCel device that you are setting up. 3 On the Profiles page, look in the Teachpoint File area to make sure the correct

teachpoint file is loaded. You will be adding new teachpoints to this file.

4 In the Controls tab, select the labware definition from the Labware list.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Setting and managing teachpoints

5 In the graphical display area, click Stacker at the top of the rack that contains the

spare microplates. In the menu that appears, click Load plates.

The BenchCel device moves the stack down and holds the stack in the stacker grippers, or supports the stack on the stacker shelf, as specified in the labware definition.

6 In the graphical display area, click the plus sign ( ) at the stacker head that

contains the spare microplates. In the menu that appears, click Downstack from Stacker.

The robot head moves under the stacker and holds the first spare microplate in its grippers.

7 On the Controls page, click the Jog/Teach tab.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Setting and managing teachpoints

8 Click Go Home. The robot head moves to its home position at the center of the

BenchCel Microplate Handler. Note: The microplate is still in the robot grippers from step 6.

9 In the Motors Enabled area, clear the check boxes for X and Theta to disable the

x-axis and theta-axis motors. The z-axis motor is still enabled (the Z box is selected).

10 With the robot grippers holding the spare microplate, gently push or pull the robot

head and arms close to the teachpoint location. Position the robot head and arms so that the microplate rests in the target microplate location.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

Click to select jog increment.

Setting and managing teachpoints

11 To fine-tune the teachpoint until you are able to place the microplate in the target

location, use the following methods:

• Visually inspect the position of the microplate relative to the plate stage. If the

robot is not aligned with the plate stage along the y-axis (front-to-back direction), move the robot into the safe zone, physically slide the external device and its integration plate along the y-axis, move the robot back and see if the robot arms are aligned with the plate stage. You might have to loosen the clamp screw to slide the device (see “About integrating devices in a BenchCel

Workstation” on page 47).

• To move the robot head up or down in small, precise increments, open

BenchCel Diagnostics. On the Controls page Jog/Teach tab, select the jog increment from the corresponding list, and then click Up () or Down ().

• With the x-axis and theta-axis motors disabled, gently push or pull the robot

side to side so that the robot arms are able to place the microplate on the plate stage.

The microplate should sit level on the target location. In addition, the robot arms should not come in contact with any part of the target platepad, plate stage, or other surfaces.

12 Visually inspect the position of the microplate on the target location. It should be

centered on the location. If it is not, ensure that the x-axis and theta-axis motors are disabled on the Jog/Teach tab, and then manually move the robot head to the microplate at the location.

BenchCel Microplate Handler User Guide

4 Setting up the BenchCel device

CAUTION

Tighten the screw to lock the devices in position.

Setting and managing teachpoints

Locking the device positions

Tightening the clamp screw can cause the teachpoint to shift. Make sure you verify the teachpoint coordinates after locking the device position.

Before you record the teachpoint, lock the devices in their positions.

To lock the devices in their positions:

1 Tighten the BenchCel integration plate clamp screw using the 3-mm hex wrench. 2 Check the teachpoint coordinates again. See “Determining the teachpoint

coordinates” on page 86.

BenchCel Microplate Handler User Guide

Agilent BenchCel Microplate Handler Original Instructions

Specifications and Main Features

Frequently Asked Questions

User Manual

Preface

About this guide

Accessing product user information

1 Safety guidelines

General safety information

Safety and regulatory compliance

Emergency stops

Safety equipment and features

Potential safety hazards

BenchCel Microplate Handler description

Hardware overview

Integration options for workstations

Labware considerations

Software description

3 Installing BenchCel Microplate Handler

Installation workflow

Verifying laboratory requirements

Unpacking BenchCel Microplate Handler

Mounting the robot

About integrating devices in a BenchCel Workstation

Connecting the power source

Connecting the pendant directly to the BenchCel device

Connecting the pendant through the Robot Disable Hub

Connecting and disconnecting the air source

Connecting the computer

Installing the shield

4 Setting up the BenchCel device

Setup workflow

Starting up and shutting down

Creating BenchCel device in the VWorks software

Opening BenchCel Diagnostics

Creating profiles

Initializing the device

Setting and managing teachpoints