The AriaDx Real-Time PCR Dx instrument is a fully integrated quantitative PCR amplification,
detection, and data analysis system for nucleic acids samples.
The instrument is to be used only by operators trained in laboratory techniques and procedures.
The customer is responsible for validation of assays and compliance with regulatory
requirements that pertain to their procedures and uses of the instrument.
Notice
Each laboratory must validate their own assays for use on the AriaDx Real-Time PCR System.
Assays must include controls designed to detect inconsistencies in instrument performance due
to, for example, changes in optical performance or thermal block uniformity.
Symbol Table
European ConformityCaution
In Vitro Diagnostic Medical DeviceCatalog/code number
ManufacturerConsult Instructions for Use
Authorized representative in the
European Community
Temperature limitation
AriaDx Real-Time PCR System Setup and User Guide2
Content
Contents
1Before You Begin
Overview of the AriaDx Real-Time PCR System 7
Materials provided with the AriaDx Real-Time PCR System 7
Hardware specifications 8
Recommended plasticware 8
Safety precautions 9
Indicator LED light 11
Agilent Technical Support 12
2Installing and Setting Up the AriaDx Instrument
Installing the AriaDx Instrument 14
Step 1. Select a location for the instrument 14
Step 2. Unpack the shipping containers 14
Step 3. Install the optical modules 16
Step 4. Clean the thermal block 19
Step 5. Connect the instrument to a power supply 20
Step 6. Connect a keyboard or mouse to the instrument (optional) 20
Step 7. Connect the instrument to a network or directly to a PC 20
Setting Up the AriaDx Instrument 23
Step 1. Turn on the instrument and calibrate the background for the optical modules 23
Step 2. Set the instrument date and time 23
Step 3. Run a diagnostic check 24
Logging In and Creating User Accounts 25
Step 1. Log in to the instrument using an Administrator account 25
Step 2. Add user accounts 26
Step 3. Log in to your personal user account 26
Customizing Instrument Settings 27
3Installing the Aria Software
Minimum requirements for running the Aria software 29
Installing the Standard Aria Software 30
Download the Aria software 30
Install the standard Aria software 30
Launch the Aria software 34
Installing the Aria ET (Electronic Tracking) Software 35
Download the Aria software and Microsoft SQL Server 2012 35
AriaDx Real-Time PCR System Setup and User Guide3
Install Microsoft SQL Server 2012 35
Install the Aria ET software 39
Configure and start Microsoft Distributed Transaction Coordinator (MSDTC) service 44
Launch the Aria ET software 47
Installing Microsoft .NET Framework 4.0 49
Download Microsoft .NET Framework 4.0 49
Install Microsoft .NET Framework 4.0 49
4Running Experiments
Preparing and Loading PCR Samples 53
Prepare samples 53
Load samples 53
Setting Up and Running Experiments 54
Use the touchscreen to set up and run an experiment 54
Use the PC software to set up and run an experiment 54
Managing Saved Experiments 56
Locate saved experiment files 56
Create a subfolder 56
Copy and paste experiments 57
Transfer experiments to a USB drive 57
5Calibrating the AriaDx System
Calibrating the Instrument Motor 59
Calibrate the motor 59
Check the motor calibration date 59
Calibrating the Background 60
Calibrate the background 60
Check the background calibration status 60
Performing Other Calibration Procedures 61
Calibrate the touchscreen 61
Calibrate the analysis of high resolution melt data 61
6Maintaining, Updating, and Disposing of the AriaDx System
Maintaining the Instrument 63
Service the instrument 63
Clean the instrument 63
Clean the optical modules 63
Updating the Instrument Software 65
Disposing of Instrument Parts 66
Cybersecurity Guidance 67
AriaDx Real-Time PCR System Setup and User Guide4
7Troubleshooting
Troubleshooting Instrument Error Messages 69
View error messages 69
Open the diagnostic report 69
AriaDx Real-Time PCR System Setup and User Guide5
AriaDx Real-Time PCR System
Setup and User Guide
1Before You Begin
Overview of the AriaDx Real-Time PCR System 7
Materials provided with the AriaDx Real-Time PCR System 7
Hardware specifications 8
Recommended plasticware 8
Safety precautions 9
Indicator LED light 11
Agilent Technical Support 12
This chapter contains information for you to read and understand before you start setting up the
instrument.
6
Overview of the AriaDx Real-Time PCR System
The AriaDx Real-Time PCR System is a fully integrated quantitative PCR amplification, detection,
and data analysis system. The system design combines a thermal cycler, an optical system with
an LED excitation source, and data analysis software. The instrument can hold up to six optics
modules, and the scanning optics design delivers optimal separation between the dyes and
between samples. The instrument provides a closed-tube PCR detection format that can be used
with a variety of fluorescence detection chemistries including SYBR® Green and EvaGreen dyes
as well as fluorogenic probe systems including TaqMan probes.
Materials provided with the AriaDx Real-Time PCR System
Table 1Materials provided
Materials providedQuantity
AriaDx instrument1
Certificate of Conformance1
AriaDx installation poster1
Power cord1
Optical modulesUp to 6 optical modules, as selected by the user
The AriaDx instrument, the Certificate of Conformance, and the AriaDx installation poster are all
shipped together in the same shipping container. The power cord and optical modules are each
shipped in their own packaging.
Table 2Materials offered as options at time of purchase
OptionsQuantity
Electronic tracking (ET) software1 seat
Notebook PC1
AriaDx Real-Time PCR System Setup and User Guide7
Hardware specifications
Table 3Hardware specifications for the AriaDx Real-Time PCR System
FeatureSpecification
Excitation sourceEight dye-specific LEDs per optical module
DetectionEight photodiodes per optical module
DyesROX, FAM, HEX, CY5, CY3, ATTO 425
Electrical power (input)100–240 VAC, 50/60 Hz, 1100VA
Thermal systemPeltier-based, 96-well block
Six slots, swappable cartridges, no reference channel needed
Dimensions19.7" W × 18.1" D × 16.5" H (50 cm × 46cm × 42cm)
On board quality checksInstrument QC checks:
WarrantyOne year warranty
Plastic consumablesLow-profile 0.2-ml tubes, strip tubes, and 96-well plates
25.0–99.9°C
Max Heating: >6°C/sec
Max Cooling: >2.5°C/sec
Accuracy: ±0.2°C or better at typical annealing, amplification, and
denaturation temperatures
maximum altitude of 2000 m
All checks: 30 minutes; Selected checks: 10–30 minutes
Calibration: 1-minute background calibration
Data loss prevention:
Data captured from all channels on each scan
Ability to store up to 5 GB on the instrument
See “Recommended plasticware” on page 8 for part numbers
Recommended plasticware
Table Table 4 lists the part numbers of Agilent plates, tubes, and other plastic consumables for
use in the AriaDx instrument.
AriaDx Real-Time PCR System Setup and User Guide8
Table 4QPCR plasticware for use in the AriaDx instrument
This product is designed for convenient and reliable operation, and to accepted standards of
safety. Its use does not entail any hazard if operated in accordance with the instructions given in
this document. However, incorrect operation may cause damage to the equipment or cause a
hazard to health. It is important that the following safety precautions are read and understood
before using the instrument.
Description
When sealing plates with adhesive seals, Agilent recommends using MicroAmp
Optical Film Compression Pads (Life Technologies, part number 4312639).
All users must read and understand the Setup and User Guide and only operate the unit in
accordance with the instructions given. Failure to follow instructions may cause the protection
provided by the instrument to be impaired.
Electrical
Standard electrical safety precautions should be applied, including the following:
•Always put the instrument in a location where, if needed, the power supply can be immediately
disconnected.
•Proper voltage (100–240 VAC) must be supplied before you turn on the instrument for the first
time.
•The device must be connected to a grounded socket. Do not operate the instrument from a
power outlet that has no ground connection.
•Do not touch any switches or outlets with wet hands.
•Turn off the instrument before you disconnect the power cord.
•Unplug the instrument before you clean any major liquid spills and before you service any of
the electrical or internal components.
•Do not connect the instrument to the same power strip as other high power-draw appliances
(e.g., refrigerators and centrifuges).
•Do not service the electrical components unless you are qualified to do so.
AriaDx Real-Time PCR System Setup and User Guide9
Fluids and Reagents
•Fill reaction vessels outside the instrument so that no fluids penetrate the instrument.
•Never cycle or incubate explosive, flammable and reactive substances in the instrument.
•You must observe the relevant safety regulations when handling pathogenic material,
radioactive substances or other substances hazardous to health.
•Do not submerge the instrument in any liquid.
Danger of Burns
•Do not touch the thermal block, inner side of heated lid and reaction vessels. These areas
quickly attain temperatures of greater than 50°C. Keep the heated lid closed until temperatures
of 30°C or lower are reached.
•Do not use any materials (plates, sealings, foils, mats) which are not sufficiently
temperature-stable (up to 120°C).
Operating Environment
•The ventilation slots of the device must remain free to vent at all times. Leave at least 10 cm of
space around the instrument.
•Keep the ambient temperature between 20°C and 30°C with humidity levels between 20% and
80% non-condensing.
•Do not operate the instrument in a hazardous or potentially explosive environment.
•Do not attempt to open the instrument door when the instrument is running an experiment.
Equipment Ratings
•Pollution degree 2
•Installation category II
•Altitude 2000 m
•Humidity 20 to 80%, non-condensing
•Electrical supply 100-240 VAC, 50/60 Hz, 1100VA
•Temperature 20°C to 30°C
•For Indoor Use Only
Electrostatic Discharge
The instrument is static sensitive. Electrostatic discharges greater than 8000 volts may interfere
with the normal operation of the USB ports on the instrument. Handling precautions are required
when working in high static environments. Wear a grounded wrist strap and take other antistatic
precautions prior to making contact with the device in high static environments. ESD
STM5.1-1998 Class 3B.
The AriaDx Real-Time PCR System complies to IEC 61326-2-6 standards that governs
Electromagnetic Compliance for IVD medical equipment. This equipment has been designed and
tested to CISPR 11 Class A. In a domestic environment it may cause radio interference, in which
case, you may need to take measures to mitigate the interference. It is therefore advisable to
choose a suitable electromagnetic environment for the AriaDx instrument prior to use.
AriaDx Real-Time PCR System Setup and User Guide10
Safety Symbols
The electrical/safety symbols described below may be displayed on the instrument.
Indicator LED light
Power on
Power off
Caution
Caution, hot surfaces
On the front of the instrument (top right corner) is a status indicator LED. Table 5 summarizes the
status codes for this LED light.
Table 5Appearance of the status indicator LED
Appearance Instrument Status
OffThe instrument is idle.
Blinking greenThe instrument is running.
Solid greenThe instrument is paused.
Blinking redThe instrument has detected an error. Check the display for an error
message containing further details.
AriaDx Real-Time PCR System Setup and User Guide11
Agilent Technical Support
Agilent Technical Support is available worldwide.
For US and Canada
Call (800)227-9770 (option 3,4,3)
Or send an e-mail to qPCR.support@agilent.com.
For all other regions
Agilent’s world-wide Sales and Support Center contact details for your location can be obtained at
www.agilent.com/en/contact-us/page.
AriaDx Real-Time PCR System Setup and User Guide12
AriaDx Real-Time PCR System
Setup and User Guide
2Installing and Setting Up the AriaDx Instrument
Installing the AriaDx Instrument 14
Step 1. Select a location for the instrument 14
Step 2. Unpack the shipping containers 14
Step 3. Install the optical modules 16
Step 4. Clean the thermal block 19
Step 5. Connect the instrument to a power supply 20
Step 6. Connect a keyboard or mouse to the instrument (optional) 20
Step 7. Connect the instrument to a network or directly to a PC 20
Setting Up the AriaDx Instrument 23
Step 1. Turn on the instrument and calibrate the background for the optical modules 23
Step 2. Set the instrument date and time 23
Step 3. Run a diagnostic check 24
Logging In and Creating User Accounts 25
Step 1. Log in to the instrument using an Administrator account 25
Step 2. Add user accounts 26
Step 3. Log in to your personal user account 26
Customizing Instrument Settings 27
This chapter contains instructions for installing and setting up the AriaDx instrument.
13
Installing the AriaDx Instrument
Step 1. Select a location for the instrument
•Locate a solid, flat clean surface for the instrument. Make sure that:
•The instrument can stand completely stable.
•The rear air slots must not be covered.
•The instrument has at least 10 cm (approximately 4 inches) to the next wall or neighboring
instrument.
•The instrument is not located near anything that could be a source of vibrations.
•The temperature (normal ambient) is between 20°C and 30°C with humidity levels between
20% and 80% non-condensing.
•The atmosphere is not explosive.
Step 2. Unpack the shipping containers
NOTE
1 Open the small shipping container that holds the power cord. Remove the power cord and set
it aside for now.
2 Make sure that the large shipping container is in the upright position (Figure 1), then cut the
four plastic straps that hold the container together.
The AriaDx instrument is shipped in two separate containers. The small container
holds the power cord. The large container holds the instrument and accessory
tray.
Any optical modules that you ordered with the instrument are each packed and
shipped separately in their own box.
AriaDx Real-Time PCR System Setup and User Guide14
Figure 1AriaDx instrument shipping container
3 Open the top flaps of the large container.
Inside the container is an accessory tray (see Figure 2), which contains the Certificate of
Conformance and the installation poster.
Figure 2Opened shipping container with accessory tray sitting on top
4 Remove the accessory tray. Unpack the installation poster from the accessory tray and use it
to guide you through the remainder of the unpacking process.
5 Remove the foam support that sits on top of the instrument in the shipping container.
6 Grip a handle on each side of the shipping container and lift up to remove the box sleeve that
surrounds the instrument.
The instrument sits on the base of the shipping container, as shown in Figure 3.
AriaDx Real-Time PCR System Setup and User Guide15
Figure 3Instrument on shipping container base, with box sleeve removed
7 Remove the plastic wrapping from the instrument.
8 Lift the instrument off of the shipping container base and set it down on its selected location
(see “Step 1. Select a location for the instrument”).
Agilent recommends that two people lift the instrument together.
Step 3. Install the optical modules
1 Open the instrument door by lifting up on the handle on the top of the instrument. Lift the door
all the way up and back.
2 Remove the piece of foam and then remove the strip of cardboard from around the thermal
block assembly (see Figure 4).
The optical module housing carrier is positioned to the left of the thermal block assembly.
Figure 4Foam insert (left) and underlying cardboard strip (right).
3 Slide the optical module housing carrier to the right until it is centered in the opening of the
instrument door (as shown in Figure 5). Use the indentation on the top of the carrier to help
slide it.
AriaDx Real-Time PCR System Setup and User Guide16
Figure 5Optical module housing carrier, centered in the instrument door opening
4 Open the lid on the optical module housing carrier.
a With your thumb and index finger, pinch together the two pieces of plastic in the indentation
on the top of the carrier (see Figure 6).
b Lift the lid all the way back to reveal the six slots for the optical modules (see Figure 7).
Figure 6Opening of the optical module housing
AriaDx Real-Time PCR System Setup and User Guide17
Figure 7Slots for the optical modules
5 Open the boxes containing the optical modules. Remove the top piece of foam from each box
(Figure 8) then remove the plastic bag containing the optical module.
Figure 8Optical module shipping box – top piece of foam removed
6 Install the optical modules into the slots.
a Open the plastic bag and remove the optical module.
b Peel off the plastic film from the edge of the optical module (see Figure 9). Once the film is
removed, do not to touch the exposed edge.
c Put the optical module into an available slot in the optical module housing. The correct
orientation for the optical module is label side up with the Agilent spark closer to the front of
the instrument (see Figure 10).
NOTE
AriaDx Real-Time PCR System Setup and User Guide18
If you are installing fewer than six optical modules, one or more of the slots in the
optical module housing will be empty. In such cases, make sure that the empty
slots are on the left-most side of the housing.
Figure 9Removal of plastic film from optical module
Figure 10 Installed optical modules
7 Lower the lid on the optical module housing until it clicks shut.
When you turn on the instrument for the first time, it will prompt you to calibrate the
background for the optical modules. Calibration is described in “Step 1. Turn on the instrument
and calibrate the background for the optical modules” on page 23.
Step 4. Clean the thermal block
With the instrument door still open, clean the outside and inside surfaces of the thermal block.
1 Lift the lid of the thermal block by pulling forward on the handle of the lid and then lifting the lid
up and away from the thermal block.
2 Using an aerosol can of compressed air, clean out the wells of the thermal block. Hold the can
8–10 cm away from the thermal block as you press the trigger.
3 Moisten a lint-free cleansing tissue with deionized H20, and gently wipe down the thermal
block and the underside of the lid. Then, close the lid of the thermal block and wipe down the
top of the lid.
4 Close the instrument door.
AriaDx Real-Time PCR System Setup and User Guide19
Step 5. Connect the instrument to a power supply
You must connect the instrument to a grounded AC outlet.
1 Plug the power cord into the power connector at the rear of the instrument.
See “Electrical” on page 9 for information on electrical safety precautions.
2 Connect the cable plug to the outlet.
Step 6. Connect a keyboard or mouse to the instrument (optional)
If desired, you can connect a keyboard or mouse to the instrument via the USB ports on the front
and back of the instrument.
•Plug the USB cable of the device into a USB port on the instrument.
Multimedia keyboards are not supported.
Step 7. Connect the instrument to a network or directly to a PC
Connecting your instrument to a PC, either directly or though a network, allows you to remotely
retrieve data from the instrument to your PC through the Aria PC software. If you do not connect
your instrument to a PC or network, you must transfer post-run experiment data by copying it
from the instrument to a USB drive (FAT format), and then from the USB drive to your PC.
To connect the instrument to a network
1 Plug one end of an ethernet cable into the ethernet port on the back of the instrument. Use a
standard Cat 6 straight/crossover ethernet cable.
2 Plug the other end of the cable into a network port.
To connect the instrument directly to a PC
1 Plug one end of an ethernet cable into the ethernet port on the back of the instrument. Use a
standard Cat 6 straight/crossover ethernet cable.
2 Plug the other end of the cable into the PC.
3 After you turn on the AriaDx instrument, set a static IP address, subnet mask, and default
gateway on the instrument using the instructions below.
You will need to wait to complete these steps until after you complete “Step 1. Turn on the
instrument and calibrate the background for the optical modules” on page 23.
a On the Home screen of the AriaDx touchscreen, press Settings.
b Press Connection Settings. If you see an error message that no network connection is
detected, press OK to close the error message.
c On the Connection Settings screen, select Use Manual Configuration.
d In the IP Address, Subnet Mask, and Default Gateway fields, enter the values shown in
Figure 11. Press OK.
AriaDx Real-Time PCR System Setup and User Guide20
Figure 11 Connection Settings
4 On the PC, set a static IP address, subnet mask, and default gateway using the instructions
below.
a Open the Control Panel to the Network and Sharing Center.
b Under View your active networks, click Local Area Connection (Figure 12). Note that the
appearance of the Network and Sharing Center screen may differ slightly from that shown
in Figure 12 depending on your current network setup.
Figure 12 Network and Sharing Center – View your active networks
The Local Area Connection Status dialog box opens.
c In the Local Area Connection Status dialog box, click Properties.
The Local Area Connection Properties dialog box opens
d In the list of items under This connection uses the following items, double-click Internet
Protocol Version 4 (TCP/IPv4) (Figure 13).
Figure 13 Local Area Connection Properties dialog box – Internet Protocol Version 4
The Internet Protocol Version 4 (TCP/IPv4) Properties dialog box opens.
e On the General tab of the dialog box, select Use the following IP address and set the IP
address, subnet mask, and default gateway to the values shown in Figure 14. Click OK to
close the dialog box.
AriaDx Real-Time PCR System Setup and User Guide21
Figure 14 Internet Protocol Version 4 dialog box – Use the following IP address
AriaDx Real-Time PCR System Setup and User Guide22
Setting Up the AriaDx Instrument
Step 1. Turn on the instrument and calibrate the background for the
optical modules
1 Press the power button located near the bottom left corner on the front of the instrument.
The instrument performs a series of health checks to ensure basic hardware functionality. If
the health checks reveal an instrument error, record the error and then perform a more detailed
diagnostic check (see “Step 3. Run a diagnostic check” on page 24). The instrument performs
the health checks each time it is powered on. When the tests are complete, the instrument
touchscreen opens to the Home screen.
Anytime you install new optical modules, a message box opens prompting you to calibrate the
background for the optical modules.
2 Press OK in the message box.
The Background Calibration screen opens.
3 Press Calibrate.
A message box opens instructing you to load a 96-well QPCR plate containing 20 l of
deionized H20 or TE buffer in each well onto the thermal block.
4 Prepare the plate and load it into the thermal block. Close the instrument door and press OK in
the message box.
See “Load samples” on page 53 for instructions on plate loading. See “Recommended
plasticware” on page 8 for ordering information on 96-well plates.
The instrument runs the calibration. At the end of calibration, a message box opens on the
touchscreen notifying you that the calibration was successful.
5 Click OK in the message box to close it.
The touchscreen returns to the Home screen. You are logged in to the Guest account.
The color touchscreen on the AriaDx instrument allows you to operate the instrument by touching
the buttons on the screen. If a mouse has been connected to the instrument, you may select
buttons by clicking. When software functions require data input from a keyboard, the touchscreen
automatically displays a virtual keyboard. You can also type in data input using a USB-connected
keyboard.
Step 2. Set the instrument date and time
1 In the bottom right corner of the touchscreen, press the time and date this is currently
displayed.
2 In the menu that opens, press Change Date & Time.
The Date & Time Settings screen opens.
3 Use the fields to set the date and time to the correct values. Press the help icon for detailed
instructions on setting the date and time on this screen.
AriaDx Real-Time PCR System Setup and User Guide23
Step 3. Run a diagnostic check
1 On the Home screen of the touchscreen, press Settings.
2 Press Instrument Diagnostic.
3 Press Run Diagnostics.
4 Mark the check box at the top of the screen labeled All Test.
5 Press Run.
A message box opens prompting you to make sure that no plate is loaded on the thermal
block.
6 Press OK to continue.
A dialog box opens displaying a schematic of the optical modules installed in the housing.
7 Mark the check boxes for all slots in the housing that contain an optical module. For empty
slots, leave the check box clear. Press OK.
The instrument begins running the diagnostic check. The first set of tests is the user
interaction tests.
8 During the user interaction tests, perform all actions and answer questions as prompted on the
touchscreen.
After the user interaction tests, the instrument runs the diagnostic tests. No user input is
required during this set of tests.
At the end of the diagnostic check, the touchscreen opens to the Diagnostic Report screen.
Figure 15 Diagnostic report banner, with the test results circled in red
9 Check the diagnostic report. The banner at the top of the report lists the number of tests that
passed out of the total number of test performed (see Figure 15).
•If all tests passed, you can close the diagnostic report.
•If any of the tests failed, contact Agilent Technical Support. See page 12 for contact
information.
AriaDx Real-Time PCR System Setup and User Guide24
Logging In and Creating User Accounts
The AriaDx instrument offers individualized user accounts, allowing each AriaDx user to log in to
the instrument with their own account and save experiments to their own user folder. Three
different access levels are available for user accounts: Administrator, User, and Guest. Each level
has specific user rights as described in Table 6.
Table 6Access levels
Access LevelFunctions allowed
Guest• Access rights to the Guest folder
User• Access rights to the Guest and personal user folder
Administrator• Access rights to the Guest folder and all user folders
• Creation and management of user accounts
• Installation of instrument software updates
• Access rights to the instrument diagnostic reports
Each time you turn on the instrument, you are automatically logged in to the Guest account. Use
of the Guest account is not recommended for organizations that prioritize file security.
Step 1. Log in to the instrument using an Administrator account
1 From the Home screen of the touchscreen, press User Login. Alternatively, from any screen of
the touchscreen, press Current User at the bottom of the screen, then press Open Login Page.
The User Login screen opens.
2 In the User Name drop-down, select the user name for an account that has Administrator
access level.
The instrument comes preloaded with a default Administrator account that you can select
here.
3 In the Password field, type the password for the account.
The default password for the Administrator user name is ADMIN.
4 Press Login.
A message box opens confirming that you logged in to the instrument. Press OK to close the
message box.
5 (Optional) Change the default password for the Administrator account.
a From the Home screen, press Settings.
b Press User Management.
c On the User Management screen, select the Administrator account and press Edit.
d In the Password and Confirm Password fields, type a new password for the account.
e Press OK to save the new password.
AriaDx Real-Time PCR System Setup and User Guide25
Step 2. Add user accounts
1 From the Home screen, press Settings.
2 Press User Management.
The User Management screen opens listing the available user names and corresponding
access levels.
3 Press Add.
The Add User screen opens.
Figure 16 Add User screen
4 In the User Name field, type a user name.
5 In the Access Level drop-down list, select an access level for the account. The options are
Administrator and User.
See Table 6 on page 25 for a description of access levels.
6 In the Password and Confirm Password fields, type a password for the account.
7 Press OK to save the account.
A message box opens confirming the creation of the new account. Click OK to close the
message box. You are returned to the User Management screen.
8 Repeat step 3 through step 7 for any additional user accounts that you want to create.
Step 3. Log in to your personal user account
Logging in to your personal account allows you to access your user folder. If you are logged in as
Guest, you must save the experiment to the Guest folder.
1 From the Home screen of the touchscreen, press User Login. Alternatively, from any screen of
the touchscreen, press Current User at the bottom of the screen, then press Open Login Page.
The User Login screen opens.
2 In the User Name drop-down, select your user name. In the Password field, type the password
for the account.
3 Press Login.
A message box opens confirming that you logged in to the instrument. Press OK to close the
message box.
To log out, press Current User at the bottom of any screen, then press Log Out.
AriaDx Real-Time PCR System Setup and User Guide26
Customizing Instrument Settings
You can access a variety of instrument settings using the buttons on the Settings screen of the
touchscreen. Each button takes you to a different screen in the touchscreen software. From each
screen, press the Help icon for more information on the settings available.
Table 7Buttons on the Settings screen
ButtonDescription
User Management – Opens the User Management screen, which has tools for
creating and managing user accounts. This screen can only be accessed when an
Administrator user is logged in to the instrument.
System Settings – Opens the System Settings screen, which has tools for accessing
and managing instrument settings including date/time, optical module information,
PC connections, experiment resumption, instrument name and calibration processes.
Software Updates – Displays software version information for the instrument. An
Administrator user can use this screen to update the AriaDx instrument software.
Hot Top Settings – Use this button to enable or disable the instrument hot top.
Connection Settings – Displays the IP address and other network information for the
instrument.
Instrument Diagnostic – Opens the Diagnostic Test screen, which contains buttons
for running instrument troubleshooting tests and viewing and reporting the results.
See “Open the diagnostic report” on page 69.
Calibrate Touch – Use this button to calibrate the touchscreen. The calibration
screen instructs you to touch a marker (+) appearing on the screen in order to
calibrate the touchscreen response functions.
AriaDx Real-Time PCR System Setup and User Guide27
AriaDx Real-Time PCR System
Setup and User Guide
3Installing the Aria Software
Minimum requirements for running the Aria software 2
Installing the Standard Aria Software 3
Download the Aria software 3
Install the standard Aria software 3
Launch the Aria software 7
Installing the Aria ET (Electronic Tracking) Software 8
Download the Aria software and Microsoft SQL Server 2012 8
Install Microsoft SQL Server 2012 8
Install the Aria ET software 12
Configure and start Microsoft Distributed Transaction Coordinator (MSDTC) service 17
Launch the Aria ET software 20
Install Microsoft .NET Framework 4.0 22
Download Microsoft .NET Framework 4.0 22
Install Microsoft .NET Framework 4.0 22
This chapter contains installation instructions for installing the Aria software on your PC.
1
Minimum requirements for running the Aria software
Before installing the Aria software, see Table 1 for the minimum PC requirements needed to run
the software.
Table 1Minimum requirements for running the Aria software
Operating systemWindows 10 – with regional format set to English (United States)
Supported architectures×64 (64 bit) supported on Windows 10
Programs*Microsoft .NET Framework 4.0
Microsoft SQL Server 2012 (required for ET software only)
Runtime components of Microsoft Visual C++ 2010 Libraries
Processor2 GHz Dual Core Processor
Working memory (RAM)2 GB (more is recommended)
Hard disk space40 GB
Display resolution1024 × 768 (1280 × 1024 is recommended)
* Installers for Microsoft .NET Framework 4.0 and Microsoft SQL Server 2012 are provided on the
AriaDx Software Download page of the Agilent website (see “Install Microsoft SQL Server
2012” on page 8 and “Installing Microsoft .NET Framework 4.0” on page 22). If you do not have
the needed Microsoft Visual C++ 2010 components, then the Aria installer will automatically
install them to your PC when you initiate installation of the Aria software.
AriaDx Real-Time PCR System Setup and User Guide2
Installing the Standard Aria Software
NOTE
If you connected your instrument directly to a PC, install the software on that PC. If you connected
your instrument to a network, install the software on a network PC. You can install the standard
Aria software on an unlimited number of PCs.
Make sure your PC meets the minimum requirements needed to run the Aria software. See
Table 1 on page 2 for a list of the minimum requirements.
The instructions in this chapter are for the installation of the standard Aria
software. If you purchased the optional 21 CFR Part 11-compatible features –
which include user authentication, database data storage, and audit trail support
– use the instructions in“Installing the Aria ET (Electronic Tracking) Software” on
page 8.
2 Under Aria Software Download, click the link for the latest version of the software.
The software request page opens in a new tab or window of your internet browser.
3 Complete the fields on the page and click Submit.
The page updates to provide a link for downloading the firmware software and a link for
downloading the Aria software.
4 Click the link to download the Aria software.
5 Save the Installer to your computer. The Installer is named Agilent Aria Software Setup X.X.exe
(where X.X is the software version).
Install the standard Aria software
To install the Aria software:
1 Double-click the file called Agilent Aria Software Setup X.X.exe (where X.X is the version number
of the software).
The Aria software installation wizard starts. Following the file extraction process, the wizard
opens to the Welcome window.
NOTE
AriaDx Real-Time PCR System Setup and User Guide3
If you receive an error message stating that Microsoft .NET Framework 4.0 needs
to be installed, cancel the installation of the Aria software and install Microsoft
.NET Framework 4.0 first. See “Installing the Standard Aria Software” on page 3.
4 In the Application Mode window, select AriaDx, then click Next.
The Setup Type window opens.
NOTE
The AriaDx mode of the software is only compatible with the AriaDx instrument.
The AriaMx mode of the software is only compatible with the AriaMx instrument.
Figure 4Agilent Aria installation wizard – Setup Type window
5 In the Setup Type window, Standard is selected by default. Leave this selection in place and
6 Designate a folder for the software files. The default folder is
C:\Program Files (x86)\Agilent\Agilent Aria.
•If you want to install the software to the default folder, click Next to continue.
•If you want to designate a different folder, click Change in the Destination Folder window. In
the dialog box that opens, browse to the desired folder, select the folder, and click Open.
Then, in the Destination Folder window, click Next to continue.
The Ready to Install window opens.
Figure 6Agilent Aria installation wizard – Ready to Install window
7 Click Install.
AriaDx Real-Time PCR System Setup and User Guide6
The wizard installs the Aria software to the folder designated in step 10. When installation is
complete, the InstallShield Wizard Completed window opens.
2 Under Aria PC Software requirements, click Server set up software for Electronic Tracking
software upgrade.
3 Save the Installer to your computer. The Installer is named
Agilent.Aria.SQLServer2012Setup.exe.
4 Under Aria Software Download, click the link for the latest version of the software.
The software request page opens in a new tab or window of your internet browser.
5 Complete the fields on the page and click Submit.
The page updates to provide a link for downloading the firmware software and a link for
downloading the Aria software.
6 Click the link to download the Aria software.
7 Save the Installer to your computer. The Installer is named Agilent Aria Software Setup X.X.exe
(where X.X is the software version).
Install Microsoft SQL Server 2012
Install Microsoft SQL Server 2012 prior to installing the Aria ET software. The Aria ET software
uses SQL Server for storage of the databases.
NOTE
To install Microsoft SQL Server 2012:
1 Double-click the file called Agilent.Aria.SQLServer2012Setup.exe.
The Aria SQL Server installation wizard opens.
AriaDx Real-Time PCR System Setup and User Guide8
You can install SQL Server on a different PC than the Aria ET software provided
that the two PCs are on the same network domain. Importantly, the Aria ET PC
and the SQL Server PC need to be joined to the same domain prior to installation
of the Aria ET software. Your network administrator can help you join the PCs to
the same domain.
The database system used by Aria ET (Microsoft SQL Server 2012) is known to be
incompatible with Microsoft Server Desktop Engine. For this reason, install SQL
Server on a PC that is not already running Microsoft Server Desktop Engine.
Figure 8Aria Microsoft SQL Server 2012 installation wizard
2 Click Next to continue.
The License Agreement window opens.
Figure 9Aria Microsoft SQL Server 2012 installation wizard – License Agreement window
3 If you accept the terms of this agreement, select I accept the terms in the license agreement
and click Next. (If desired, click Print before clicking Next to print a copy of the license
agreement.)
The Instance Configuration window opens.
AriaDx Real-Time PCR System Setup and User Guide9
Figure 10 Aria Microsoft SQL Server 2012 installation wizard – Instance Configuration window
4 In the Instance Name field of the Instance Configuration window, type a name for the server
instance you want to install. Hover your cursor over the Information icon next to the field to see
guidelines on selecting a valid instance name.
5 In the Enter Password field, type a password for the server instance. Hover your cursor over
the Information icon next to the field to see guidelines on selecting a valid password. Type the
password again in the Confirm Password field.
6 Click Next in the Instance Configuration window.
The Ready to Install window opens.
Figure 11 Aria Microsoft SQL Server 2012 installation wizard – Ready to Install window
AriaDx Real-Time PCR System Setup and User Guide10
7 Click Install.
The Installing window opens and remains open until installation is complete.
Figure 12 Aria Microsoft SQL Server 2012 installation wizard – Installing window
When installation is complete, the SQL Server 2012 Installation Completed window opens.
Figure 13 Aria Microsoft SQL Server 2012 installation wizard – SQL Server 2012 Installation
Completed window
8 Click Finish to close the wizard.
AriaDx Real-Time PCR System Setup and User Guide11
Install the Aria ET software
To install the Aria ET software:
1 Double-click the file called Agilent Aria Software Setup X.X.exe (where X.X is the version number
of the software).
The Aria software installation wizard starts. Following the file extraction process, the wizard
If you receive an error message stating that Microsoft .NET Framework 4.0 needs
to be installed, cancel the installation of the Aria software and install Microsoft
.NET Framework 4.0 first. See “Installing the Standard Aria Software” on page 3.
1 Click Next to continue with the installation.
The License Agreement window opens.
AriaDx Real-Time PCR System Setup and User Guide12
3 In the Application Mode window, select AriaDx, then click Next.
The Setup Type window opens.
NOTE
AriaDx Real-Time PCR System Setup and User Guide13
The AriaDx mode of the software is only compatible with the AriaDx instrument.
The AriaMx mode of the software is only compatible with the AriaMx instrument.
Figure 17 Agilent Aria installation wizard – Setup Type window
4 In the Setup Type window, select Electronic Tracking. Click Next.
5 The Software Activation window displays the Licensing Host ID. Follow the instructions
provided on your Software Entitlement Certificate to use this Licensing Host ID to redeem your
software license and save the file to your PC. Then, in the Software Activation window, click
Browse to open a dialog box where you can browse to the folder containing the license file.
Select the file and click Open.
The dialog box closes and you are returned to the Software Activation window.
6 Click Next.
AriaDx Real-Time PCR System Setup and User Guide14
The Database Server window opens.
Figure 19 Agilent Aria installation wizard – Database Server window
7 In the drop-down list at the top of the Database Server window, select the database server
instance that you set up while installing SQL Server (see step 4 on page 10).
8 In the Password field, type the password for the database server instance that you entered
while installing SQL Server (see step 5 on page 10).
9 Select a database to use as the primary database when you log in to the Aria ET software.
•To create a new database, select Create new database. Type a name for the database into
the adjacent field or use the default database name.
•To select an existing database, select Select existing database. Click Browse, and in the
dialog box that opens, select the database that you want to use and click OK. The dialog
box closes.
After you make your selection, click Next in the Database Server window.
The Destination Folder window opens.
AriaDx Real-Time PCR System Setup and User Guide15
AriaDx Real-Time PCR System Setup and User Guide16
The wizard installs the Aria software to the folder designated in step 10. When installation is
complete, the InstallShield Wizard Completed window opens.
You can now open these files in the Aria software.
AriaDx Real-Time PCR System Setup and User Guide21
Installing Microsoft .NET Framework 4.0
In order to install the Aria software, you must have Microsoft .NET Framework 4.0 or greater
installed on your PC. If you attempted to install the Aria software and received an error message
stating that Microsoft .NET Framework 4.0 is needed, follow the instructions in this section to
install this software.
2 Under Aria PC Software requirements, click Microsoft .NET Framework 4.0 Installer.
3 Save the Installer to your computer. The Installer is named dotnetfx40_full_x86_x64.exe.
Install Microsoft .NET Framework 4.0
1 Make sure your PC is connected to the internet.
2 Double-click the dotNetFx40_Full_setup.exe file.
The Microsoft .NET Framework 4 Setup window opens, displaying the software license.
AriaDx Real-Time PCR System Setup and User Guide22
Figure 26 Microsoft .NET FrameWork 4 Setup – License window
3 If you accept the terms of this agreement, select I have read and accept the license terms and
click Install. (If desired, click the Print icon or Save icon before clicking Install to print or save a
copy of the license agreement.)
The installer installs Microsoft .NET FrameWork 4.0. During installation, the Microsoft .NET
FrameWork 4 Setup window remains open and displays progress bars for the installation
tasks. When installation is complete, the Installation Is Complete window opens.
AriaDx Real-Time PCR System Setup and User Guide23
Figure 27 Microsoft .NET FrameWork 4 Setup – Installation Is Complete window
4 Click Finish to close the Microsoft .NET FrameWork 4 Setup window.
AriaDx Real-Time PCR System Setup and User Guide24
AriaDx Real-Time PCR System
Setup and User Guide
4Running Experiments
Preparing and Loading PCR Samples 53
Prepare samples 53
Load samples 53
Setting Up and Running Experiments 54
Use the touchscreen to set up and run an experiment 54
Use the PC software to set up and run an experiment 54
Managing Saved Experiments 56
Locate saved experiment files 56
Create a subfolder 56
Copy and paste experiments 57
Transfer experiments to a USB drive 57
This chapter contains instructions on preparing and running experiments and managing
experiment files on the instrument.
52
Preparing and Loading PCR Samples
Prepare samples
When preparing the PCR reactions, follow the guidelines below for optimal results.
•Use only temperature-stable PCR tubes and plates. See “Recommended plasticware” on
page 8 for a list of recommended tubes and plates.
•Place caps on tubes before loading samples into the thermal block.
•Spin samples briefly in a centrifuge immediately before loading them into the thermal block.
Load samples
The instrument can be loaded with individual PCR tubes or strip tubes or one 96-well PCR plate.
1 Open the instrument door that covers the thermal block assembly by lifting up on the handle
on the top of the instrument. Lift the door all the way up and back.
2 Lift the heated lid by pulling forward on the handle of the lid and then lifting the lid up and away
from the thermal block.
3 Put your plate or tubes on the block and check that they are correctly positioned.
4 Close the heated lid and push it backwards so that it latches into place.
5 Close the instrument door so that it latches into place.
WARNING
Danger of Burns: The thermal block, sample tubes and plates may reach temperatures
as high as 100°C. Keep hands away until temperature is 30°C or less.
AriaDx Real-Time PCR System Setup and User Guide53
Setting Up and Running Experiments
You can set up the plate and thermal profile for an experiment on either the instrument
touchscreen software or on the Aria software on your PC. The instructions below provide the
basic steps required to set up an experiment and start running the experiment. For more detailed
information about setting up and running experiments, see the help system in the Aria PC
software.
Use the touchscreen to set up and run an experiment
1 (Optional) Log in the instrument. See “Step 3. Log in to your personal user account” on page 26.
Logging in to your personal account allows you to save the experiment to your user folder. If
you are logged in as Guest, you must save the experiment to the Guest folder.
2 On the Home screen, press New Experiment.
The Experiment Types screen opens.
3 Create the experiment using one of the following approaches.
•Press the desired experiment type.
The Plate Setup screen opens.
•Press Open Template. The Template screen opens. Press a template file to select it then
press Open. (The only template files listed are those that were previously saved to the
instrument.)
The Plate Setup screen opens.
4 On the Plate Setup screen, set up the wells of the plate. Press the help icon for help with
working on the Plate Setup screen.
5 Press the Thermal Profile tab.
The Thermal Profile screen opens.
6 Set up the thermal profile for the experiment. Press the help icon for help with working on the
Thermal Profile screen.
7 Load the samples onto the thermal block (see “Load samples” on page 53), and press Run
Experiment on the Thermal Profile screen.
A message box opens asking you to save the experiment. Click OK to open the Save
Experiment screen.
8 Select a folder for the experiment file and press Save.
The Raw Data Plots screen opens, allowing you to monitor the progress of the run.
Use the PC software to set up and run an experiment
1 On the Getting Started screen, create the experiment using one of the following approaches.
•Click Experiment Types. The screen displays the experiment types. Click the desired
experiment type to select it. Type a name for the experiment and click Create. The Plate
Setup screen opens.
AriaDx Real-Time PCR System Setup and User Guide54
•Click My Templates. The screen displays the templates in the default template folder. Click
the desired template to select it. Type a name for the experiment and click Create. The
Plate Setup screen opens.
•Click From LIMS file. The screen displays a wizard for importing a LIMS data file. Use the
wizard to import a saved LIMS data file and describe the new experiment. Upon completing
the wizard, the Plate Setup screen opens.
2 On the Plate Setup screen, set up the wells of the plate. See the software help system for help
with setting up the plate.
3 Click Thermal Profile in the Experiment Area on the left side of the screen.
The Thermal Profile screen opens.
4 Set up the thermal profile for the experiment. See the software help system for help with
setting up the thermal profile.
5 Click Run.
The Instrument Explorer dialog box opens.
6 In the dialog box, locate the instrument and click Send Config.
•If you have not already logged in to the selected instrument, you will be prompted to log in
before continuing.
•If you have not already saved the experiment you will be prompted to save it before
continuing.
7 Load your samples onto the thermal block (see “Load samples” on page 53).
8 At the bottom of the instrument touchscreen, press the icon shown below.
In the pop-up menu that opens, press Open Primed Experiment.
The experiment opens on the touchscreen.
9 On the Thermal Profile screen of the touchscreen, press Run Experiment.
The Raw Data Plots screen opens, allowing you to monitor the progress of the run.
AriaDx Real-Time PCR System Setup and User Guide55
Managing Saved Experiments
Each user account, including the Guest account, has its own user folder on the AriaDx instrument.
Users can save and retrieve experiment files to and from the folders to which they have access.
(All users have access to the Guest folder and the folder for their account. Administrator accounts
have access to all folders on the instrument. Users logged in as Guest can only access the Guest
folder and the HRM Calibration folder.)
Locate saved experiment files
1 If necessary, log in the instrument. See “Step 3. Log in to your personal user account” on
page 26.
Logging in to your personal account allows you to access experiment files in your user folder.
If you are logged in as Guest, you can only access experiment files in the Guest folder and the
HRM Calibration folder (which only contains HRM calibration experiments).
2 On the Home screen of the touchscreen, press Saved Experiment.
The Experiment Explorer screen opens. The left side of the screen lists the folders to which you
have access.
3 Press directly on a folder to select it.
The right side of the screen lists the experiment files and any subfolders in the selected folder.
Experiment files have the file extension adxd.
4 To open a subfolder, double-press the subfolder on the right side of the screen.
The subfolder expands to display the experiment files and subfolders contained within.
Create a subfolder
1 On the Experiment Explorer screen, locate the folder in which you want to create a subfolder.
Press the folder to select it.
See “Locate saved experiment files”, above, for instructions on opening and navigating the
Experiment Explorer screen.
2 Press New Folder.
The virtual keyboard appears.
3 In the New Folder Name field, type a name for the subfolder. Press Create Folder.
The new subfolder appears on the Saved Experiment screen.
AriaDx Real-Time PCR System Setup and User Guide56
Copy and paste experiments
1 On the Experiment Explorer screen, locate the experiment that you want to copy. Press the
experiment to select it.
See “Locate saved experiment files” on page 56 for instructions on opening and navigating the
Saved Experiment screen.
2 Press Copy.
3 Browse to the folder to which you want to paste the experiment file. Press the folder to select
it.
4 Press Paste.
The experiment file is pasted to the selected folder.
Transfer experiments to a USB drive
1 Insert a FAT format USB drive into the USB port on the front of the instrument.
2 On the Experiment Explorer screen, locate the experiment that you want to copy. Press the
experiment to select it.
See “Locate saved experiment files” on page 56 for instructions on opening and navigating the
Saved Experiment screen.
3 Press Copy.
4 On the left side of the Experiment Explorer screen, press the folder for the USB drive (called
USBDisk).
5 Press Paste.
A copy of the experiment file is saved to the USB drive.
NOTE
You can also transfer experiments from the instrument to the Aria PC software
by connecting the instrument directly to the PC or a network. See page 20 for
instructions setting up PC or network connections. See the help system for the
Aria PC software for instructions on retrieving experiments from a connected
instrument.
AriaDx Real-Time PCR System Setup and User Guide57
AriaDx Real-Time PCR System
Setup and User Guide
5Calibrating the AriaDx System
Calibrating the Instrument Motor 59
Calibrate the motor 59
Check the motor calibration date 59
Calibrating the Background 60
Calibrate the background 60
Check the background calibration status 60
Performing Other Calibration Procedures 61
Calibrate the touchscreen 61
Calibrate the analysis of high resolution melt data 61
This chapter contains instructions for calibration procedures that can be performed on the AriaDx
Real-Time PCR System.
58
Calibrating the Instrument Motor
Motor calibration is performed prior to shipping a new instrument, and you do not need to
calibrate it again unless the instrument prompts you to do so.
Calibrate the motor
Instruments are provided with the motor calibration step completed, and additional motor
calibration runs are not generally required after receipt of the instrument. If, however, you see a
notification on the instrument touchscreen alerting you that motor calibration is required, press
Calibrate, then follow the on-screen prompts.
NOTE
Changing the installed optical modules does not affect the motor calibration.
Check the motor calibration date
You can check the date and time of the most recent motor calibration run.
1 On the Home screen, press Settings.
2 On the Settings screen, press System Settings.
3 On the System Settings screen, press Motor Calibration.
This screen displays the date and time that motor calibration was run for the instrument.
AriaDx Real-Time PCR System Setup and User Guide59
Calibrating the Background
Background calibration determines the level of background noise associated with each optical
module and compensates for that background. Anytime you install new optical modules, a
message box opens on the touchscreen prompting you to calibrate the background for the optical
modules.
Calibrate the background
When replacing the optical module installed in any slot with a different module, the touchscreen
will prompt you to run the Background Calibration for the newly-installed module.
1 When a message box opens on the touchscreen prompting you to run background calibration,
press Calibrate.
The Background Calibration screen opens.
2 Prepare the PCR plate for the calibration run.
a Add 20 l of water or TE buffer (10 mM Tris/1 mM EDTA) into all 96 wells of a PCR plate.
Cap the wells or seal with film.
b Spin the plate briefly to collect the fluid at the bottom of the wells.
c Insert the plate in the thermal block, close the hot top, and press Calibrate on the
touchscreen.
When the instrument has completed the calibration, the Background Calibration screen will be
updated to show the revised calibration status for each optical module.
3 Press Cancel to return to the System Settings screen.
Check the background calibration status
You can check the date and time of the most recent background calibration run for each optical
module that is currently installed.
1 On the Home screen, press Settings.
2 On the Settings screen, press System Settings.
3 On the System Settings screen, press Background Calibration.
This screen displays the background calibration status and other properties of the optical
module installed in each slot of the instrument. To view the properties of a specific installed
optical module, press the bar corresponding to that module slot on the left side of the screen.
AriaDx Real-Time PCR System Setup and User Guide60
Performing Other Calibration Procedures
The other calibration procedures available on the AriaDx system include calibrating the
touchscreen and
Calibrate the touchscreen
You can calibrate the touchscreen response functions in order to optimize sensitivity.
1 On the Home screen, press Settings.
2 On the Settings screen, press Calibrate Touch.
The next screen instructs you to touch a marker (+). Follow the prompts provided on the
screen.
Calibrate the analysis of high resolution melt data
For experiments that include a high resolution melt (HRM) segment, you must associate the
experiment with an HRM calibration plate (HCP) that was run on the same instrument. See the
help system for the Aria software for instructions on running an HCP.
AriaDx Real-Time PCR System Setup and User Guide61
AriaDx Real-Time PCR System
Setup and User Guide
6Maintaining, Updating, and Disposing of the AriaDx
System
Maintaining the Instrument 63
Service the instrument 63
Clean the instrument 63
Clean the optical modules 63
Updating the Instrument Software 65
Disposing of Instrument Parts 66
Cybersecurity Guidance 67
This chapter contains instructions for maintaining the instrument, uploading software updates,
and disposing of instrument parts.
62
Maintaining the Instrument
The AriaDx instrument is designed to require a minimum amount of maintenance by the user.
Service the instrument
As preventative maintenance, Agilent recommends having your AriaDx instrument serviced by an
Agilent service engineer every 12 months. Contact Agilent technical support for information on
instrument service. See “Agilent Technical Support” on page 12 for contact information.
Clean the instrument
To clean the exterior of the AriaDx instrument:
1 Turn off and disconnect the instrument from the power supply.
2 Using laboratory wipes moistened with 70% isopropyl alcohol, clean the exterior surfaces of
the instrument listed below. Do not let liquid enter the instrument.
•All surfaces of the door including the depression at the top that is used to open the door
•The surface of the touchscreen
•Both sides of the instrument
•The power button and adjacent area
•The front panels of the instrument
•Allow isopropyl alcohol to evaporate before reconnecting the instrument to the power supply.
Clean the optical modules
To clean an optical module:
1 Remove the optical module from its slot in the optical module housing.
a Open the lid on the optical module housing carrier.
b Lift the hinged tab on the top of the optical module (see Figure ), then use the tab to lift the
optical module out of its slot.
AriaDx Real-Time PCR System Setup and User Guide63
Figure 44 Lifting the hinged tab on an optical module
2 Using an aerosol can of compressed air, clean the bottom surface of the optical module (the
surface opposite of the label). Hold the can 3–4 inches away from the surface as you press the
trigger.
3 (Optional) Wipe the bottom surface with a lens cloth or lens tissue moistened with
reagent-grade isopropyl alcohol or reagent-grade acetone.
4 Re-install the optical module into the housing. Lower the hinged tab until it snaps into place.
5 Close the lid on the housing carrier.
AriaDx Real-Time PCR System Setup and User Guide64
Updating the Instrument Software
You will be notified by Agilent when a new version of the instrument software is available. This
notification includes a web address where you can download the software files. Save these files
to a USB memory stick and then upload them to your instrument using the instructions below.
1 Login to one of the Administrator user accounts. See “Step 1. Log in to the instrument using an
Administrator account” on page 25.
2 From the Home screen, press Settings and then press Software Update.
3 Insert the USB memory stick into the USB port on the front of the instrument.
4 Press Browse to open a browser that allows you to navigate to the folder containing the
software files. Select the appropriate folder and press OK to return to the Software Update
screen.
5 Press Update.
The instrument begins the update. When the update is complete, the instrument automatically
reboots.
6 When the reboot is complete, you may return to the Software Update screen to confirm the
new version of the software is running.
AriaDx Real-Time PCR System Setup and User Guide65
Disposing of Instrument Parts
If, at any time, the AriaDx instrument or any of its parts, including the optical modules, are no
longer of use, you can return the unneeded items to Agilent as part of the Agilent Product
Take-Back program. See http://www.agilent.com/environment/product/index.shtml for
information on this program.
AriaDx Real-Time PCR System Setup and User Guide66
Cybersecurity Guidance
The Electronic Tracking (ET) option for the Aria software provides the ability for user account
management, including creating, changing, safeguarding, and terminating accounts. The
HealthCare Organization shall create corresponding internal procedures to address the following
items:
1 All the network equipment, computer hardware, software, databases etc. must be protected by
user authentication (ID and password).
2 System should be accessible though domain as well as local user.
3 System should use Windows and/or Hospital Information (IT) infrastructure password policy.
4 System should support role management access. Access to files and drives should be given
for appropriate role.
5 System should allow appropriate users to
•Create new users
•Add/remove privileges to user
•Terminate/disable user
•Create groups with defined privileges
•Add/remove users to/from group.
6 System should be locked after predefined time of inactivity.
7 All systems should have anti-virus and anti- spyware software installed.
8 The anti-virus and anti-spyware software should be updated regularly.
9 Important data should be secured using Cryptography or encryption- decryption equipment.
10 Data transmitted to and from devices must be encrypted.
11 Unauthorized devices trying to connect to the network must be blocked.
12 Unused ports should be blocked to minimize risk of tampering.
13 Firewall should be set to prevent unauthorized access from internet/intranet.
14 The use of mobile devices must follow mobile device management policy.
15 Documented procedures for addressing security breaches must beavailable.
16 Authorized user accounts must be regularly audited.
17 Database backup must be performed on a regular basis and must be performed before
installing any software upgrades.
18 Employees must be educated on privacy and security policies through a strict training process.
19 The number of remote computers, portable devices and ports used to connect to IVD systems
should be kept to a minimum.
20 Software must be kept up- to- date and any available patches promptly installed.
21 A documented security plan for disposal of unwanted data must be available.
22 Policies and processes for data security must be regularly reviewed and updated.
AriaDx Real-Time PCR System Setup and User Guide67
AriaDx Real-Time PCR System
Setup and User Guide
7Troubleshooting
Troubleshooting Instrument Error Messages 69
View error messages 69
Open the diagnostic report 69
This chapter contains instructions for troubleshooting instrument errors.
68
Troubleshooting Instrument Error Messages
When the instrument detects issues with the hardware, firmware, or optical modules, it notifies
you with an error message. Error messages are also prompted when the instrument encounters
an issue while running the scanning application feature or while performing diagnostic tests.
View error messages
When the AriaDx instrument encounters an error, it notifies you by displaying an error icon at the
bottom of the touchscreen.
1 Press the error icon at the bottom of the touchscreen.
2 In the pop-up menu that opens, press Count X, where X is the number of error messages to be
read.
A table opens displaying the following information.
•Type - The type of message (Error or Warning). Warning messages are used for errors that
do not prevent you from running experiments on the instrument.
•ID - The error code ID number.
•Description - A description of the error with instructions on how to resolve it.
3 Press OK to close the table.
Open the diagnostic report
Some error messages may instruct you to refer to the diagnostics report for further details about
the cause of an error.
1 Log in using an Administrator account.
See “Step 1. Log in to the instrument using an Administrator account” on page 25.
2 On the Home screen, press Settings.
The Settings screen opens.
3 Press Instrument Diagnostic.
The Diagnostic Test screen opens.
4 Press View Result.
The Diagnostic Report screen opens. The table lists each category of diagnostic test included
on the report.
5 Double-press a category to expand its contents.
The table displays the tests within that category. The Results column indicates if the test
passed or failed. The Run Date column lists the date and time of the test.
AriaDx Real-Time PCR System Setup and User Guide69
www.agilent.com
Manufactured by
Agilent Technologies LDA Malaysia Sdn. Bhd.
Bayan Lepas Free Industrial Zone
11900 Penang, Malaysia
No part of this manual may be reproduced in any form or by any
means (including electronic storage and retrieval or translation into
a foreign language) without prior agreement and written consent
from Agilent Technologies, Inc. as governed by United States and
international copyright laws.
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Version F0, March 2021
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