Agilent 6100 Series Quadrupole LC/MS
Systems
Quick Start Guide
Where to find more information 1
Configuration Diagrams 3
ChemStation Views 5
Basic Operation 18
Deconvolution 23
Operating Tips 25
Troubleshooting Tips 27
This manual applies to OpenLAB CDS ChemStation Edition Software (version
C.01.03 and later until superseded) and Agilent 6100 Series LC/MS
instruments.
Where to find more information
Online Help Use online Help for in-depth information beyond what is shown
in this Quick Start Guide. Display online Help in any of the following ways:
• Click the Help button in the toolbar.
• Select Help Topics from the Help menu.
• Click the Help button on most dialog boxes to show task-specific help.
What’s New in C.01.06?
User Guides Besides this Quick Start Guide, the following guides are shipped
with your system and are also available in PDF format on the installation disk.
• Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
• Agilent 6100 Series Quadrupole LC/MS Systems Concepts Guide
• Agilent 6100 Series Quadrupole LC/MS Systems Installation Guide
• Agilent 6000 Series LC/MS Maintenance Guide
What’s New in C.01.06?
• 5 kDa/sec and 10 kDa/sec Fast Scan speeds on G6120B and G6130B with
• 5 kDa/sec, 8 kDa/sec, and 16 kDa/sec Ultrafast Scan speeds on G6150B
step size of 0.2 Da. Multiple Fast Scan speeds, such as 5k Da/sec and 10k
Da/sec., are now supported for Data Acquisition.
with step size of 0.2 Da. Multiple Ultrafast Scan speeds, such as 5 kDa/sec
and 8 kDa/sec or 8 kDa/sec, and 16 kDa/sec are now supported for Data
Acquisition.
What's New in C.01.03?
• Support for Windows 7 32-Bit and 64-Bit with Service Pack 1.
• Support for Fast Scan on G6120B.
• Support for Fast Polarity Switching on G6120B (hardware upgrade
required).
• Support for ESI with Agilent Jet Stream Technology on G6130B (hardware
upgrade required).
What's New in C.01.02?
• Support for Windows 7 32-Bit and 64-Bit.
• Support for 6100 Series LC/MS on OpenLAB CDS ChemStation Edition.
2 Agilent 6100 Series LC/MS System Quick Start Guide
Configuration Diagrams
1100/1200/1260/1290 Series LC
Solvent cabinet
Pump
Configuration Diagrams
1100/1200/1260/1290 Series LC
Solvent bottles
Vacuum degasser
Control module
Autosampler
LC Detector
(DAD or VWD)
Agilent 6100 Series LC/MS System Quick Start Guide 3
(optional)
Column
compartment
Configuration Diagrams
LC Cabling
LC Cabling
Remote cable
CAN bus cable
CAN bus cable
AC power
Analog signal
to recorder
Shielded LAN cable
to network hub
4 Agilent 6100 Series LC/MS System Quick Start Guide
ChemStation Views
The LC/MS ChemStation software consists of several views. Click one of the
view buttons in the lower left corner of the screen, or from the View menu, to
change to a different view.
Method and Run Control View Allows you to set up methods and adjust
instrument parameters to inject samples and acquire data one sample at a
time or in automated sequences. See the next page for more information.
ChemStation Views
LC Cabling
Data Analysis View Allows you to perform various data evaluation tasks on
chromatograms and spectra, such as integration, quantitation, checking peak
purity, deconvolution, and report generation. See page 9 for more information.
Report Layout View Allows you to design custom report templates to use with
the data generated by your ChemStation. See page 12 for more information.
Verification (OQ/PV) View For use by Agilent service engineers to determine
if your system is operating in a predictable manner. This view is useful to
show Good Laboratory Practice (GLP) compliance, which some government
agencies can require. See page 14 for more information.
Diagnosis View Allows you to run tests to diagnose instrument problems and
access information on how to resolve these problems. The early maintenance
feedback (EMF) feature can notify you when it is time to perform system
maintenance before a problem occurs. See page 16 for more information.
MSD Tune View In this view, you can calibrate your LC/MS automatically. You
can also set MS parameters manually for specific types of molecules. See
page 17 for more information.
Agilent 6100 Series LC/MS System Quick Start Guide 5
ChemStation Views
Method and Run Control View
Method and Run Control View
Tool Set Selection:
Sequence or Single
Sample
Run
Control
System
Diagram
File List
View
Selection
Online Plot
Message Line
Method Overview
Method Sequence
Top Toolbar
Status Bar
MS, FIA
Data
Analysis
Disk Space
NOTE
The user interface for Method and Run Control can be configured in two ways during
installation. See Agilent 6100 Series Single Quad LC/MS System Installation Guide for
information on installation and configuration.
If your instrument is configured with the Classic user interface, then see ChemStation
online Help and these manuals on the ChemStation disk:
• Agilent 6100 Series Single Quad LC/MS Systems Quick Start Guide
• Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide
• Agilent 6100 Series Quadrupole LC/MS Systems Concepts Guide
6 Agilent 6100 Series LC/MS System Quick Start Guide
ChemStation Views
Method and Run Control View
Status and Run
Bars: Instrument
Control Tab
Right-click the Method icon to see the following commands:
Run Time Checklist, Method Information, Edit Entire Method, Method Audit
Trail, Print Method, and Help.
Start
Sequence
Stop
Sequence
Method
System Diagram Each icon of the system diagram represents one component or module of your
system. Click on an icon if you want to edit the method parameters or go to the
online Help for that particular component.
Injector
Pump & Solvents Column
Detectors
Agilent 6100 Series LC/MS System Quick Start Guide 7
ChemStation Views
Method and Run Control View
Single Sample
Tool Set
Sequence
Tool Set
Flow Injection
Analysis (FIA)
This toolset allows you to work on methods and run an analysis for a single
sample. Move the cursor over a button to view a description of it.
Click the Single Sample icon to display this toolset.
This toolset allows you to work on sequences and run automated analyses of
multiple samples. Move the cursor over a button to view a description of it.
Click the Sequence icon to display this toolset.
This toolset allows you to inject multiple samples directly into the detector,
which lets you bypass the chromatographic column. The results are sent to a
single data file. FIA can be used for method development or for applications
that do not require chromatography.
To enable FIA from the system diagram:
• Click the FIA icon.
• Select Edit FIA Series.
• Mark the Enable FIA Series in Method option and edit the
FIA Table.
8 Agilent 6100 Series LC/MS System Quick Start Guide
Data Analysis View
ChemStation Views
Data Analysis View
File List
Tool Set Selection
Signal View Selection
Display Graphics
Tools
DAD Signal
LC/MS Signal
Message Line
Navigation Tools
Cursor Tools
The various tool sets available in Data Analysis View are shown on the
following pages. You can view a description of a button by moving the cursor
over it.
Agilent 6100 Series LC/MS System Quick Start Guide 9
ChemStation Views
Data Analysis View
Common Tool Set This toolset is always present in Data Analysis view.
Graphics Tool Set Allows you to manipulate the graphic display.
Integration Tool Set Allows you to perform integration and reporting tasks on
a chromatogram.
10 Agilent 6100 Series LC/MS System Quick Start Guide
ChemStation Views
Data Analysis View
Calibration Tool Set Allows you to perform calibration tasks for quantitation.
Signal Tool Set Allows you to work graphically with the UV or MS signal.
Purify Tool Set Allows you to work graphically with your purification data.
Spectral Tool Set Allows you to perform spectral evaluation tasks.
Deconvolution Tool Set Allows you to transforms mass spectra from
multiply-charged ions into a calculated molecular weight.
Agilent 6100 Series LC/MS System Quick Start Guide 11
ChemStation Views
Report Layout View
Report Layout View
New Section Menu
Insertion Triangle
Click a blue triangle to activate
the new section menu (triangle
turns red)
Message Line
Report Layout
Tool Set This toolset is present in the Report Layout view. Move the cursor
over a button to view a description of it.
Report Template
Calculation Technique
Toolbar
Report Section
Tips • Edit or create a report layout as described in the online Help.
• To test your report layout, select a calculation technique from the
drop-down list box to define how the results are calculated.
• Load a data file. The results are loaded into the report template using the
selected calculation.
• Select File / Add to Report Styles to add your completed report template
to the list of available report styles. Now you can use your customized
report within a method.
12 Agilent 6100 Series LC/MS System Quick Start Guide
Sample Report Layouts (in \CHEM32\REPSTYLE)
Template Description
SIMPLE.FRP Includes a chromatogram and calibration curves per calibrated
compounds.
NESTED.FRP Shows how to nest subsections.
SPECTRA.FRP Shows how to include spectra.
ChemStation Views
Report Layout View
PURITY.FRP Shows how to include peak purity data.
ESTD.FRP Uses the same elements as the standard ESTD reports so you can
customize your external standard reports.
AREAPCT.FRP Uses the same elements as the standard area percent report so you can
customize your area percent reports.
Print Preview button
Agilent 6100 Series LC/MS System Quick Start Guide 13
ChemStation Views
Verification (OQ/PV) View
Verification (OQ/PV) View
Task
Selection
Menu
System
Diagram
Start/Stop Run
Verification Toolbar
Message
Line
The Verification (OQ/PV) view lets Agilent service engineers test whether your
analytical instruments and the ChemStation software are operating correctly
according to predefined performance criteria.
Operation Qualification (OQ) Operation Qualification is the documented
verification that the equipment-related system or subsystem performs as
intended throughout representative or anticipated operating ranges.
14 Agilent 6100 Series LC/MS System Quick Start Guide
ChemStation Views
Verification (OQ/PV) View
Performance Verification (PV) Performance Verification is the documented
verification that the process and or the total process-related system performs
as intended throughout all anticipated operating ranges.
System Diagram Each icon of the system diagram represents one component
or module of your system.
Verification Toolbar The Verification toolbar is displayed when you select
Show Top Toolbar from the View menu. Move the cursor over a button to view
a description of it.
Available Tests The following verification tests are supplied with your
ChemStation software for use by an Agilent service engineer. Refer to the
online Help for more information on these tests.
• VWD Wavelength Accuracy
• Intensity
• Holmium
• Temperature Accuracy
• Noise, Flow, Temperature
• DAD Wavelength Accuracy
• Injector Precision
• Detector Linearity/Carry-over
• Injector Linearity
• Gradient Composition
Agilent 6100 Series LC/MS System Quick Start Guide 15
ChemStation Views
Diagnosis View
Diagnosis View
Task
Selection
Menu
Instrument
Panel
View
Selection
Message Line
Variables Display
Symptoms Cause
Possible Causes
Information Pad
This view allows you to carry out diagnostic and maintenance activities on
your system.
Diagnosis Toolbar The Diagnosis toolbar is displayed when you select Show
Top Toolbar from the View menu. Move the cursor over button to view a
description of it.
16 Agilent 6100 Series LC/MS System Quick Start Guide
MSD Tune View
ChemStation Views
MSD Tune View
File Selection
View Selection
Message Line
Title Bar
MSD Tune Toolset
MSD Tune Toolbar
Status Bar
This toolset is present in MSD Tune view.
Load an LC/MS tune file. Calibrate the mass axis.
Save the current tune file. Change spray chamber
parameters.
Generate a profile and scan
report.
Autotune the LC/MS and print a
tune report.
Edit LC/MS parameters for
manual tuning.
Acquire multiple scans and send
results to data file.
Agilent 6100 Series LC/MS System Quick Start Guide 17
Basic Operation
Start up and shut down the system
Basic Operation
Start up and shut down the system
Task Instruction Comment
To start up the system 1 From the Method and Run Control
view, click the button on the
system status bar. When you move
mouse over the button, if system is
currently idle, it turns green.
2 Set up the LC conditions (pump,
column heater, and detector).
3 Set up the LC/MS spray chamber
conditions as described in the MSD
Spray Chamber dialog box topic in
the online Help.
Allow 15 minutes for the system to warm
up.
To put the system in Standby Mode 1 Flush the system with pure mobile
phase to ensure that the flow path is
clear of buffers. This prevents the
nebulizer from plugging. A plugged
nebulizer can cause a high back
pressure, which can damage the LC
flow cell.
It is good practice to flush the flow
path (including the column) for 5 to 10
minutes with a mobile phase without
buffers such as 50:50
water/acetonitrile.
2 Click the button on the system
status bar. All modules are set to
standby mode (indicated by the gray
color in the system diagram).
This procedure assumes that the
system is under vacuum and the LC
and LC/MS are properly connected. It
also assumes that the liquid flow path
is properly set up.
Put the system in standby mode
overnight or whenever you are not
analyzing samples for an extended
time. The standby state for the LC/MS
leaves the nebulizer and drying gas on
at low flow.
Standby for the LC/MS is:
• 20 psi for the nebulizer,
• 3 L/min for drying gas,
• 300 °C for drying gas temperature,
• 325 °C for the APCI vaporizer, if
present
• the MS stream selection valve is
set LC to Waste.
For Agilent Jet Stream source:
• Sheath temp 100°C
• Sheath gas flow 2.2 L/minute
18 Agilent 6100 Series LC/MS System Quick Start Guide
Tune the LC/MS
When the LC/MS is used as a detector for the LC, a mass spectrum is
associated with each data point in the LC chromatogram. To obtain high
quality, accurate mass spectra, optimize the LC/MS to:
• Maximize sensitivity
• Maintain acceptable resolution
• Ensure accurate mass assignment
Tuning is the process of adjusting LC/MS parameters to achieve these goals.
After the LC/MS parameters have been optimized, they must be saved in a
tune file (.tun). This tune file is then specified in the method that is used to
acquire data for your samples.
Frequent tuning, whether automated or manual, is not required. Once tuned,
the LC/MS is stable. Tuning is needed no more often than monthly, weekly at
most.
Wait 4 hours after pumpdown before tuning or operating your LC/MS. The
analyzer takes about 11 hours to reach thermal equilibrium. Tune files that
are created or data that is acquired before the LC/MS is at thermal
equilibrium can have incorrect mass assignments and other inaccuracies.
Basic Operation
Tune the LC/MS
Task Instructions Comments
To use Autotune 1 From the MSD Tune view, select
Autotune from the Tune menu or click
the Autotune toolbar button.
2 Review the tune report that is printed
automatically when tuning is
completed.
Use autotune for automated
adjustment of the LC/MS
performance.
Agilent 6100 Series LC/MS System Quick Start Guide 19
Basic Operation
Tune the LC/MS
Task Instructions Comments
To use Check Tune 1 Select Check Tune from the Tune
menu.
2 Review the Check Tune report.
Adjustments are suggested for values
that are outside of acceptable ranges.
To use Manual Tune 1 Select Manual Tune from the Tune
menu.
2 Set the desired mass resolution
(adjusting width gain and width
offset).
3 Calibrate the mass axis (adjusting
mass gain and mass offset).
4 Optimize the ion transmission through
the source ion optics (fragmentor,
skimmer(s), lens 1, lens 2, and
octopole peak).
5 Adjust the signal strength (setting iris
and adjusting the multiplier gain).
Fragmentor and gain are method
parameters. The fragmentor affects ion
transmission and fragmentation. For
more information, see the online Help.
Use Check Tune to determine quickly
whether the LC/MS is correctly tuned.
It performs a single profile scan of the
tune masses and compares the peak
widths and mass axes with target
values.
Use Manual Tune when you want to:
• Achieve maximum sensitivity by
sacrificing some resolution
• Tune specifically for the very low
end (<150 amu) of the mass range
• Tune with a compound other than
the standard calibrants
20 Agilent 6100 Series LC/MS System Quick Start Guide
Acquire LC/MS Data
Modes of Acquisition
There are three modes of acquiring data:
• Running a method for a single sample
• Running a sequence for multiple samples
• Running an FIA series
Note the following about acquiring data:
• All three acquisition modes require an appropriate method.
• Samples can be injected either manually or with an ALS.
• Always start a run from the software.
• An FIA method cannot be used in a sequence but multiple FIA methods can
be run sequentially.
Basic Operation
Acquire LC/MS Data
Agilent 6100 Series LC/MS System Quick Start Guide 21
Basic Operation
Acquire LC/MS Data
To edit a method and start a run
Once you know the acquisition mode that you want to use, you must set up an
appropriate method. Methods are set up in the Method and Run Control view.
Instructions Comments
1 Activate the single sample toolset by clicking the
button on the toolbar.
2 Load a method by selecting Method / Load Method
or by clicking the button on the toolbar and
selecting a method from the list.
3 Click the button to begin editing the method. You
can also begin by selecting Edit Entire Method from
the Method menu or by right-clicking the icon
on the Run Control Bar.
4 Select the method sections you want to edit (select all
sections to become familiar with the method
parameters that are available) and click OK.
5 Add any method comments you want to appear on
your reports.
6 Set up the instrument parameters, such as pump,
injector, DAD or VWD, column thermostat, MS
signals, and MS spray chamber.
7 Set up the Data Analysis parameters, such as signal
details, integration events, report parameters,
instrument curves, calibration curves, calibration
table, and ion parameters.
8 Complete the Run Time Checklist.
9 Save the method using a different name. Select
Method / Save Method As or click the button on
the toolbar.
10 When you are ready to begin a run, click the Start
button.
A series of dialog boxes is displayed,
which allows you to set up your
method and instrument parameters.
Click the Help button on any of the
method dialog boxes for descriptive
information on the items available in
each.
Once you are familiar with the options
that are available, use the system
diagram menus for quick access to
particular method parameters.
22 Agilent 6100 Series LC/MS System Quick Start Guide
Deconvolution
Deconvolution is an optional process (additional license required) that
transforms mass spectra from multiply-charged ions into a calculated
molecular weight.
To deconvolute a mass spectrum
Instructions Comments
1 In the Data Analysis view, load an electrospray MS
Deconvolution
To deconvolute a mass spectrum
data file. The TIC is displayed.
2 Generate the MS spectrum that you want to
deconvolute. (Select the Spectral task tool set and use
the spectrum selection tools to pick the spectrum of
interest).
3 Click the Enter Deconvolution Tool button to
set up the deconvolution display area.
4 When the spectrum is displayed, click the
Label Ions button to locate masses to be
used in deconvolution.
5 Examine the ions that were found. Very noisy data or
data with unresolved regions often need special
settings to find ions.
6 Click the Edit Deconvolution Parameters
button to change any of the parameters.
7 Click the Run Deconvolution button to begin
the deconvolution process.
These operations are performed on
data acquired in full scan mode.
You can use the Ion Label
Options button to optimize
the set of ions found.
Once the deconvolution is done, the
components are displayed in the
upper right window. The charge states
are displayed in the lower right
window.
Agilent 6100 Series LC/MS System Quick Start Guide 23
Deconvolution
Deconvolution Report
Instructions Comments
8 Select specific components from the Component list
to look at individual components or groups of
components.
9 Optionally, select components from the
Component List box and then click the
Delete Components button.
10 Repeat the process.
Deconvolution Report
The Deconvolution report contains summary information about each
component selected, along with detailed information on how each peak in a
component contributes to the molecular weight of the component. In the first
part of the report, the components are ranked by percent relative abundance,
which is useful for estimating the percent of impurities.
The actual molecular weight can differ from the computed molecular weight
even if the data fit a Gaussian curve perfectly, due to other errors such as
errors in mass axis assignment or unresolved chemical impurities.
Optionally, click the Preview
Deconvolution Report
button
or click the Print Report
button.
This action removes the peaks for the
selected components from the original
spectrum. You can then continue with
step 4 (previous), using this new
spectrum.
The Deconvolution software is optional and is part of the OpenLAB CDS
ChemStation C.01.xx or M8363AA (ChemStation C.0x) Bioanalysis Software
package.
24 Agilent 6100 Series LC/MS System Quick Start Guide
Operating Tips
• Back up your data and methods regularly to avoid loss of data in case the
• Put the system in standby mode overnight or whenever you are analyzing
• Verify that the tune file you are using is appropriate for your samples.
• Save Tune reports in an MS Logbook for future reference.
• Regular system maintenance can reduce problems. Keep a maintenance
• Use the Maintenance Logbook and EMF features in Diagnosis view to help
• Flush the sample path and clean the spray chamber, capillary tip, and spray
• The spray chamber vent hose must be connected to a lab vent that is used
• Samples must be filtered. If no chromatography is used, they must be salt
• If a UV detector is available, use it in series with the LC/MS. Try to
• To avoid chromatographic band broadening, be sure that all tubing
• Use the following table as a guide to using SIM, condensed scan, and full
Operating Tips
files are accidentally overwritten, deleted, or if a hardware problem
develops with your disk drive.
samples for an extended time.
record.
you track when maintenance is needed and to keep an online maintenance
record.
shield daily or at the end of each shift. Check the foreline pump fluid level
every week.
only for the source. The vent hose must be separate from the vent hose for
the foreline pump. Otherwise, waste products can migrate into the spray
chamber vent producing chemical noise.
and detergent-free.
minimize chromatographic peak broadening by using low dispersion tubing.
connections are free of dead volume. Use zero dead-volume (ZDV) fittings
when possible.
scan acquisition modes.
Task Mode
Acquire electrospray data for samples containing large,
multiply-charged analytes.
Analyze a mixture with unknown components (small molecules). Scan
Agilent 6100 Series LC/MS System Quick Start Guide 25
Full Scan
Operating Tips
Task Mode
Analyze a mixture with known components in unknown amounts
(quantitate).
Identify the presence of a few known compounds at low levels
within a mixture.
Use fast chromatography on 1.0-MHz LC/MS systems (G6120B,
G6130B) without missing peaks.
Use fast chromatography on 1.4-MHz LC/MS systems (G6150B)
without missing peaks.
Scan or SIM
SIM
Fast Scan
Ultra Fast Scan
26 Agilent 6100 Series LC/MS System Quick Start Guide
Troubleshooting Tips
No peaks
✔ Verify that there is spray from the nebulizer.
✔ Verify that the capillary voltage is set correctly.
✔ In APCI mode, verify that the corona needle is installed.
In APCI mode, verify that the Corona Current setting is 2 μA or higher, and
that the Corona Voltage is between 1500 and 5000 V.
✔ Verify that the MS system is tuned correctly.
✔ Verify that MS vacuum pressures are within normal ranges.
✔ Check the drying gas flow and temperature.
✔ Verify that the fragmentor is set correctly.
✔ Check if any error message indicates a problem with electronics.
✔ Verify that sufficient sample is present in vial.
Troubleshooting Tips
Poor mass accuracy
✔ Recalibrate the mass axis.
✔ Verify that the ions used for tuning span the mass range of the sample ions
and that they show strong, stable signals.
Low signal
✔ Check the solution chemistry. Verify that the solvent you are using is
appropriate for your sample. Mixed samples can exhibit signal suppression
of one or more components.
✔ Verify that the sample is fresh and has been stored correctly.
✔ Verify that the MS system is tuned correctly.
✔ Check the nebulizer condition.
✔ Clean the capillary entrance.
✔ Check the capillary for damage and contamination.
Agilent 6100 Series LC/MS System Quick Start Guide 27
Troubleshooting Tips
✔ Verify that the spray chamber parameters are set correctly, such as drying
gas heater and nebulizer pressure.
28 Agilent 6100 Series LC/MS System Quick Start Guide
Troubleshooting Tips
Unstable signal
✔ Verify that the drying gas flow and temperature are correct for the solvent
flow you are using.
✔ Verify that the solvent is thoroughly degassed. Do not use ultrasonic
degassing with protein samples.
✔ Verify that the LC backpressure is steady; steady backpressure indicates a
steady solvent flow.
High spectral noise
✔ Use appropriate mass filter values.
✔ Check the spray shape. Nebulizer can be damaged or incorrectly set.
✔ Verify that drying gas flow and temperature are correct for the solvent flow
you are using.
✔ Verify that the solvent is thoroughly degassed. Do not use ultrasonic
degassing with protein samples.
✔ Verify that the LC backpressure is steady; steady backpressure indicates a
steady solvent flow.
✔ If you are using water as part of the mobile phase, verify that it is deionized
(>18M).
Droplets, not spray, exiting the nebulizer
✔ Verify that the nebulizing gas pressure is set high enough for the LC flow
being used.
✔ Check the position of the needle in the nebulizer.
✔ Stop the solvent flow and remove the nebulizer assembly. Use a magnifying
glass to examine the end of the nebulizer for damage.
Agilent 6100 Series LC/MS System Quick Start Guide 29
Troubleshooting Tips
No flow
✔ Verify that the LC pump is on and there is sufficient solvent in the correct
bottle.
✔ Verify that the solvent supply lines are free of air bubbles. Purge the
appropriate pump channels, if needed.
✔ Check for LC error messages.
✔ Check for blockages. Repair or replace any blocked components.
✔ Check for leaks.
✔ Verify that the MS stream selector valve is set to LC to MSD.
Undesired fragmentation
✔ Fragmentor is set too high.
✔ Ionization is causing fragmentation (APCI vs. Electrospray).
✔ APCI temperature is too high.
30 Agilent 6100 Series LC/MS System Quick Start Guide
Safety Warnings
Intended Purpose
Use of the LC/MS in a manner not intended by the manufacturer is prohibited.
Safety Class
The LC/MS is a Safety Class 1 instrument. It has been designed and tested in
accordance with IEC Publication 1010-1, Safety Requirements for Electrical
Equipment for Measurement, Control, and Laboratory Use.
Stack Configuration
Safety Warnings
WARNING
WARNING
WARNING
WARNING
Do not stack more than three LC modules of any kind on top of the LC/MS
instrument. Stacking more than three LC modules is potentially unstable and
dangerous.
Placing the LC modules on top of the LC/MS is also inconvenient. It puts the
solvent bottles and some LC controls out of easy reach and requires the LC
stack to be disassembled and removed for LC/MS maintenance.
Use only Nitrogen as the drying and nebulizer gas. Air, oxygen, or other gases,
when combined with solvents and high voltages in the spray chamber, can result in
an explosion.
Do not connect the LC/MS to a power source that is not equipped with a protective
earth contact. You can create a shock hazard for the operator and can damage the
instrument.
Do not interrupt the protective conductor inside or outside the LC/MS or disconnect
the protective earth terminal. You can create a shock hazard for the operator and
can damage the instrument.
Agilent 6100 Series LC/MS System Quick Start Guide 31
Safety Warnings
WARNING
WARNING
Do not adjust, maintain, or repair the opened instrument while it is connected to a
power source. Only trained persons who are aware of the hazards involved can
perform these operations.
Before you perform maintenance on the electronics vent and turn off the LC/MS
and disconnect the power cord. The power switch on the front of the LC/MS does
not completely cut power to the instrument.
32 Agilent 6100 Series LC/MS System Quick Start Guide
Safety Warnings
Agilent 6100 Series LC/MS System Quick Start Guide 33
www.agilent.com
In this book
This book contains:
• Where to find more
information
• What’s New in C.01.06?
• Configuration Diagrams
• ChemStation Views
• Basic Operation
• Operating Tips
• Troubleshooting Tips
• Safety Warnings
Agilent Technologies, Inc. 2014
Printed in USA
Revision A, March 2014
*G1960-90082*
G1960-90082
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