Advanced 3250, 4250 User manual

Advanced

Osmometer Model 3250

Cryoscope Model 4250

Service Manual

3255SM Rev22 020416

Table of Contents

1. Introduction

Specifications . . . . . . . . . . . . . . . . . . . . .7

General Description and Purpose . . . .11

Service Assistance . . . . . . . . . . . . . . . .11

Safety/Regulatory Information . . . . . .12

Symbol Conventions . . . . . . . . . . . . . .12

General Cautions . . . . . . . . . . . . . . . . .12

Service & Maintenance Cautions . . . .13

Hazardous Material Cautions . . . . . . .14

Regulatory Notices . . . . . . . . . . . . . . .14

Symbol Conventions . . . . . . . . . . . . . .14

FCC Requirements . . . . . . . . . . . . . . .15

2. General Overview

General Overview . . . . . . . . . . . . . . . .21

Calibration . . . . . . . . . . . . . . . . . . . . . .23

Circuit Description . . . . . . . . . . . . . . .24

Power Supply . . . . . . . . . . . . . . . . . . . .24

Front End . . . . . . . . . . . . . . . . . . . . . . .24

325605 Control Board Set . . . . . . . . . .24

325620/425620/325621/425621

Processor Board . . . . . . . . . . . . . . . .25

PCB605 Application Board . . . . . . . . .26

Other Circuit Components . . . . . . . . . .27

Design Changes . . . . . . . . . . . . . . . . . .29

Replacement Parts . . . . . . . . . . . . . . . .30

Supplies & Accessories . . . . . . . . . . . .32

3. Maintenance

Cleaning and Maintenance . . . . . . . . .35

Removing the Head & Instrument

Covers . . . . . . . . . . . . . . . . . . . . . . .36

Osmometer/Cryoscope Well Cleaning 37 Cleaning and Lubricating the Printer .39 Probe & Stir/Freeze Wire Alignment .40

4. Troubleshooting

Introduction . . . . . . . . . . . . . . . . . . . . .43

Freeze “Buzz” Amplitude . . . . . . . . . .44

Stir Driver Noise . . . . . . . . . . . . . . . . .45

Head Sensor Adjustment . . . . . . . . . . .47

Head Up/Down Test . . . . . . . . . . . . . .48

Power On Diagnostics . . . . . . . . . . . . .49

A/D Tests . . . . . . . . . . . . . . . . . . . . . . .49

Sample Bin Setting . . . . . . . . . . . . . . .50

Block Bin Setting . . . . . . . . . . . . . . . .51

Stir Amplitude . . . . . . . . . . . . . . . . . .51

Display/Printer Test . . . . . . . . . . . . . . .51

Beeper Test . . . . . . . . . . . . . . . . . . . . .52

Keypad Test . . . . . . . . . . . . . . . . . . . . .52

Barcode Test . . . . . . . . . . . . . . . . . . . .52

Reset Serial Number . . . . . . . . . . . . . .52

Instrument Software Updates . . . . . . .54

Performing FLASH firmware update .54

Troubleshooting Table . . . . . . . . . . . . .56

5. Replacement Instructions

Block Probe (4D3340) . . . . . . .325P340

Clapper (3C2241R) . . . . . . . .3C2P241R

Control Board Set (325605R/425605R),

Application Board (PCB605R), Processor Board (325621R/

425621R) . . . . . . . . . . . . . .325P620

Cooling Assembly (3D3300R/

4D3300R) . . . . . . . . . . . .325P300

Cooling Fan (4D3360) . . . . . . .325P360

Display Board (74051R/

702041R) . . . . . . . . . . . .325P051

Driver Board (PCB523R) . . .PCB523PM

Fuse (70011/70022) . . . . . . . . . . .325P11

Head Up Relay or Head Down

Relay (3D3109) . . . . . . . . . .325P109

Heat Transfer Fluid Pump

Assembly (4D3690), Fluid Filter (4D3710R), One-way Check

Valve (4D3705R/99133R) . . . .4DP690

Keypad (325511R) . . . . . . . . . .325P511

Load Resistor (425150R) . . . . .42P150R

Mandrel (3LH500) . . . . . . . . . .3LHP500

Motor Assembly (4D3350R/

325304R) . . . . . . . . . . . . . .325P350

NVRAM Battery (71027R) . . . .71P27R

Power Supply Assembly (4D3950/

325950R) . . . . . . . . . . . . . .325P950

Printer Assembly (325400R) . . .325P401 Printer Assembly (325420R) .325420PM Printer Mechanism

(325404R) . . . . . . . . . . . . . .325404PM

Probe Alignment Tool Kit

(3LA700) . . . . . . . . . . . . . . . . .3LP700

Sample Probe (3D3700) . . . . . . .3DP700

Sample Probe (3D3700) . . . . . .325P700

Sample Probe (4D3102) . . . . . . .4D3P02

Cryoscope Sample Probe

Versions FAQ . . . . . . . . . . . . .4DP002

Stir/Freeze Coil (3D2404R) . . .325P404

The Advanced®Model 3250/4250 Service Manual

Stir/Freeze Wire (3LH243/

4LH243) . . . . . . . . . . . . . . . .4LHP243

Transistor (PCB520R) . . . . . . .PCP520R

Transistor (PCB522R) . . . . . . .PCP522R

Tube Ejector Spring (3D3312R/

4D3312) . . . . . . . . . . . . . . . 4DP312

Upgrade Kit, 3250/4250 Suffix

D to E (325024) . . . . . . . . .325025PM

Yoke (3LH230R) . . . . . . . . . . .3LHP230

6. Schematics

PCB402 Assembly Drawing PCB402 Printer Interface Board

Schematic PCB402 Schematic PCB523 Assembly Drawing PCB523 Driver Board Schematic PCB523 Schematic PCB605 Assembly Drawing PCB605 Application Board Schematic PCB605 Schematic PCB620 Assembly Drawing PCB620 Processor Board Schematic 210102 Assembly Drawing 210102 Schematic 3D3380 Assembly Drawing 3D3380 Schematic 325000 Interconnection Schematic

APPENDICES

A: Symbol Definitions

B: Product Disposal and Recycling C: Service Log

Index

The Advanced®Model 3250/4250 Service Manual

1. Introduction

Model 3250 Osmometer Specifications

Applies to Serial Suffix A - C. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 130 VAC (1.2 A) or 200 to 250

VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 2-Amp time delay for 100-130 VAC;

1-Amp time delay for 200-250 VAC

Power: 150 Watts

Memory Backup: integral lithium cell; 10 years

min. (not user-replaceable)

Sample Volume: 0.2 or .25 mL

Sample Capacity: single sample

Readout: 20-character digital display

Units: mOsm/Kg H2O

Range:

Low: 0 to 2000 mOsm/Kg H2O High: 1400 to 4000 mOsm/Kg H2O

Resolution: 1 mOsm/Kg H2O

Performance Over Operating Conditions

Temperature Effects: Less than 1 mOsm/Kg H

for every 5°C (9°F) ambient temperature change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 2 minutes per sample

in 0 to 2000 mOsm range; moderately longer in high range.

Dimensions inches centimeters

Width: 13 32 Depth: 18 46 Height: 16 41

Communications:

RS-232C and barcode scanner ports

Weight pounds kilograms

Net: 28 13 Shipping: 39 19

Performance at Reference Conditions

Warranty: One-year limited warranty on work-

Linearity: Less than ±0.5% from a straight line

manship and all parts except glass, plastic and parts warranted by their

Repeatability:

makers.

0 to 400 mOsm ±2 mOsm/Kg (1 S.D.) 400 to 4000 mOsm ±0.5% (1 S.D.)

Drift: Less than 1 mOsm/Kg H

O per

Certification:

month

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I Over-Voltage Category: II Pollution Degree: 2 Moisture Protection: IPX0 (Ordinary)

Reference Conditions: 20 to 25°C (68 to 77°F); 40 to 60% Relative Humidity; tolerances of reference or calibration solutions

excluded.

The Advanced®Model 3250/4250 Service Manual

Model 3250 Osmometer Specifications

Applies to Serial Suffix D and Higher. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 250 VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 2-Amp time delay (Type T)

Power: 95 Watts

Memory Backup: integral lithium cell; 10 years

min. (not user-replaceable)

Sample Volume: 0.2 or .25 mL

Sample Capacity: single sample

Readout: 20-character digital display

Units: mOsm/Kg H2O

Range:

Low: 0 to 2000 mOsm/Kg H2O High: 1400 to 4000 mOsm/Kg H2O

Resolution: 1 mOsm/Kg H2O

Communications:

RS-232C and barcode scanner ports

Performance at Reference Conditions

Linearity: Less than ±0.5% from a straight line

Repeatability:

0 to 400 mOsm: Std. Deviation < 2 mOsm/Kg H2O 400 to 4000 mOsm: Std. Deviation < 0.5% of value

mOsm/Kg H2O

Drift: Less than 1 mOsm/Kg H

O per

month

Performance Over Operating Conditions

Temperature Effects: Less than 1 mOsm/Kg H

for every 5°C (9°F) ambient temperature change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 2 minutes per sample

in 0 to 2000 mOsm range; moderately longer in high range.

Dimensions inches centimeters

Width: 13 32 Depth: 18 46 Height: 16 41

Weight pounds kilograms

Net: 23 10 Shipping: 34 15

Warranty: One-year limited warranty on work-

manship and all parts except glass, plastic and parts warranted by their makers.

Certification:

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I Over-Voltage Category: II Pollution Degree: 2 Moisture Protection: IPX0 (Ordinary)

Reference Conditions: 20 to 25°C (68 to 77°F); 40 to 60% Relative Humidity; tolerances of reference or calibration solutions

excluded.

The Advanced®Model 3250/4250 Service Manual

Model 4250 Cryoscope Specifications

Applies to Serial Suffix A - C. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 130 VAC (1.2 A) or 200 to 250

VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 250V time delay (Type T):

2-Amp time delay for 100-130 VAC; 1-Amp time delay for 200-250 VAC

Power: 150 Watts

Memory Backup: integral lithium cell; 10-year

life (typical); (not userreplaceable)

Sample Volume: 2.0 to 2.5 mL

Sample Capacity: single sample

Readout: 20-character vacuum fluorescent

display

Units: mºC or mºH

Range: 0 to 1000 mºC or mºH

Resolution: 1 mºC or mºH

Communications: On-board printer, DTE EIA-

232/V.24 (RS-232) serial port and optional barcode scanner

Performance at Reference Conditions

Linearity: Less than ±0.5% from a straight line

Performance Over Operating Conditions

Temperature Effects: Less than 1 unit for every

5°C (9°F) ambient tempera ture change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 90 seconds in 30-

second timed mode.

Dimensions inches centimeters

Width: 13.0 33.0 Depth: 18.0 45.7 Height: 12.0 30.5

with Head Up: 16.0 40.6

Weight pounds kilograms

Net: 28.0 12.7 Shipping: 39.0 17.7

Warranty: One-year limited warranty on work-

manship and all parts except glass, plastic and parts warranted by their makers.

Repeatability:

± 2 mºC or mºH (1 S.D.) between 0 and -400 mºC ± 0.5% (1 S.D.) between -400 and -1000 mºC

Drift: Less than 1 unit per month

Certification:

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I

Over-Voltage Category: II

Pollution Degree: 2

Moisture Protection: IPX0 (ordinary)

The Advanced®Model 3250/4250 Service Manual

Model 4250 Cryoscope Specifications

Applies to Serial Suffix D and Higher. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 250 VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 2-Amp time delay (Type T)

Power: 95 Watts

Memory Backup: integral lithium cell; 10-year

life (typical); (not userreplaceable)

Sample Volume: 2.0 to 2.5 mL

Sample Capacity: single sample

Readout: 20-character vacuum fluorescent

display

Units: mºC or mºH

Range: 0 to 1000 mºC or mºH

Resolution: 1 mºC or mºH

Communications: On-board printer, DTE EIA-

232/V.24 (RS-232) serial port and optional barcode scanner

Performance at Reference Conditions

Performance Over Operating Conditions

Temperature Effects: Less than 1 unit for every

5°C (9°F) ambient tempera ture change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 90 seconds in 30-

second timed mode.

Dimensions inches centimeters

Width: 13.0 33.0 Depth: 18.0 45.7 Height: 12.0 30.5

with Head Up: 16.0 40.6

Weight pounds kilograms

Net: 23 10 Shipping: 34 15

Linearity: Less than ±0.5% from a straight line

Repeatability:

0 to -400 mºC: Std. Deviation <

-400 to -1000 mºC: Std. Deviation < 0.5% of value mºC or mºH

Drift: Less than 1 unit per month

The Advanced®Model 3250/4250 Service Manual

2 mºC or mºH

Warranty: One-year limited warranty on work-

manship and all parts except glass, plastic and parts warranted by their makers.

Certification:

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I

Over-Voltage Category: II

Pollution Degree: 2

Moisture Protection: IPX0 (ordinary)

General Description and Purpose

Service Assistance

Advanced

Osmometers and Cryoscopes

determine the concentration of solutions through freezing-point depression measurement. Osmometers are used primarily to measure body fluids such as serum and urine, and cryoscopes are used to determine the freezing points of various liquids and to detect added water in milk. Application information is available upon request.

Except for different cooling assemblies, stir/freeze wires, sample sizes and software, the 3250 and the 4250 are identical to one another. For clarity when replacing parts or troubleshooting, the instructions indicate any significant differences between the two instruments.

The Service Manual

This Service Manual contains:

To contact Advanced Instruments Product Service:

• Call (800) 225-4034 (toll-free within the USA and Canada; after normal business hours, dial extension 2191)

• Call +US (781) 320-9000 (elsewhere)

• Or fax (781) 320-3669

When calling Advanced Instruments, have the model and serial number from the label on the back of the instrument, and a description of the problem. Use a telephone as close to the instrument as possible in order to facilitate making recommended diagnostic checks.

A service technician may assist in making minor repairs over the phone, providing you with recommended parts (or part numbers), or may issue an authorization (RMA) to ship the instrument for factory repair.

• instructions for performing repairs.

• guides for troubleshooting operational and mechanical problems.

• circuit system illustrations.

• information about replacement parts and service repairs.

This manual is designed to assist service technicians, and does not imply a license to perform repairs without proper qualifications.

The RMA procedure is as follows:

1. Contact Advanced Instruments to obtain an RMA.

2. Pack and ship the instrument in its original carton. Do not send instrument supply items.

3. Prepay shipment to the factory. Advanced Instruments cannot accept collect ship-

ments. Insure the shipment or accept the damage risk.

The Advanced®Model 3250/4250 Service Manual

Safety / Regulatory Information

To reduce the risk of bodily injury, electric shock, fire, and damage to your instrument, please read and observe the following precautions.

• If the product is used in a manner not in accordance with the equipment design, operating instructions or manufacturer's recommendations, the operation of the product may be impaired to the extent that a safety hazard is created.

• Do not attempt to perform electrical work if you are not fully qualified. This manual is not a substitute for electrical training.

Symbol Conventions

The exclamation point within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance (servicing) instructions in the literature accompanying this product.

The lightning flash with arrowhead symbol within an equilateral triangle is intended to alert the user to the presence of uninsulated dangerous voltage within the product's enclosure that may be of sufficient magnitude to constitute risk of electric shock to persons.

The static symbol within an equilateral triangle is intended to alert the user to the presence of internal components that could be damaged by static electricity.

This symbol indicates the presence of a fuse.

This symbol indicates the presence of protective earth ground.

This symbol indicates the power is ON.

This symbol indicates the power is OFF.

NOTE Additional symbol definitions are

available in the Symbols section.

General Cautions

• This product should be operated only with the type of power source indicated on the product’s electrical ratings label. Refer to the installation instructions included with the product.

• If the power cord provided is replaced for any reason or if an alternate cord is used, the cord must be approved for use in the local country. The power cord must be approved for the product’s listed operating voltage and be rated at least 20% greater than the ampere ratings marked on the product’s electrical ratings label. The cord end that connects to the product must have an IEC 60320 connector.

• Plug the product into an approved grounded electrical outlet.

This static symbol is intended to alert the user to the presence of a specific component that could be damaged by static electricity.

This symbol indicates the presence of alternating current (AC).

The Advanced®Model 3250/4250 Service Manual

• Do not disable the power cord’s grounding plug.

• If an extension cord or power strip is used, make sure that the cord or strip is rated for the product, and that the total ampere ratings of all products plugged into the extension cord or strip do not exceed 80% of the cord’s or strip’s rating limit.

• Route power cords so that they will not be walked on, tripped on, or pinched by items placed upon or against them. Pay particular attention to the plug, electrical outlet, and the point where the cord exits the product.

• Do not pull on cords and cables. When unplugging cords or cables, grasp the corresponding connector.

• Do not install or use this product in any area subject to extreme short-term temperature variations, or locations that exceed the specified operating environment temperatures.

• Never use this product in a wet area.

- The power cord, extension cord, power strip or power input module is damaged.

- Liquid has been spilled into the interior of the product.

- A foreign object has fallen into the product.

- The product has been dropped or damaged by a falling object.

- There are noticeable signs of overheating or a burning odor.

- The product does not operate normally when you follow the operating procedures.

• To avoid injury or fire hazard, do not operate this product in an explosive atmosphere.

• Do not install or use the product on an unstable, non-level work surface.

• Do not operate this product with the covers removed or unsecured.

Service & Maintenance Cautions

• Unplug the power cord prior to opening or removing covers, or else you may be exposed to electric shock, excessive temperatures, or mechanical hazards.

• Performing service or maintenance not detailed in the User’s Guide, with or without this Service Manual, should only be done by a qualified service technician.

• Never restrict airflow into or out of the product. Occasionally, check the air vents

for blockage.

• Wipe the exterior of the product with a

soft, damp cloth as needed. Using cleaning products other than those specified, may discolor or damage the finish.

• If the product requires service for any of

the following reasons, unplug the product from the electrical outlet and refer service to a qualified service technician.

- The main supply fuse(s) or any internal fuse(s) continually fail.

• A discharge of static electricity from contact with the human body or other conductor may damage system boards or static sensitive devices. Never perform internal maintenance without following recommended static protection procedures.

• The product is equipped with operator accessible fuses. If a fuse blows, it may be due to a power surge or failure of a component. Replace the fuse only once. If the fuse blows a second time, it is probably caused by failure of a component part. If this occurs, refer service to qualified service personnel. Always replace the fuse with one of the same rating, voltage, and type. Never replace the fuse with one of a higher current rating.

• When servicing the product, use only factory-specified parts.

• WARNING: When returning this product for service, or shipping this product to a second location, remove all hazardous specimens and decontaminate the product before packaging for shipment. If the product cannot be decontaminated, consult with your shipping agent on appropriate packaging and marking.

The Advanced®Model 3250/4250 Service Manual

Hazardous Material Cautions

• WARNING: Handle all biohazardous materials according to established good laboratory practices and follow your institution’s exposure control plan. Persons handling human blood and body fluid samples must be trained in blood-borne hazards and observe universal precautions. Universal precautions is an approach to infection control, where all human blood and body fluids are treated as if known to be infectious. Use personal protective equipment such as gloves, gowns, etc., to prevent exposure. Store biohazardous materials in regulated waste containers and dispose of these materials in a safe and acceptable manner that is in compliance with all country, state and local requirements.

• If a biohazardous material is spilled on or inside the equipment, decontaminate the equipment using a 1% bleach solution, or as outlined by those policies and procedures established within your institution.

• To avoid injury or fire hazard, do not operate this product in an explosive atmosphere.

Regulatory Notices

• This product has been designed and manu-

factured in accordance with U.S., Canadian, and European regulatory requirements as outlined below. Modifications made to this product that are not expressly approved in writing by the manufacturer will void the user’s authority to operate this product, previously issued factory approvals, and the user’s rights under the warranty.

• The distributor or dealer may have applied

additional local, national, or international approvals to this product. Consult the distributor or dealer for more information and documentation.

• Connections to this product must be made with shielded cables. Use of non-shielded cables may violate RFI/EMI limits.

Symbol Conventions

This symbol indicates conformity to relevant European directives.

This symbol indicates the product was tested to conform to relevant Canadian and U.S. safety standards by Intertek Testing Services NA, Inc. The ETL mark is approved in the United States as a Nationally Recognized Testing Lab (NRTL) by OSHA, and in Canada by the Standards Council of Canada.

In Vitro Diagnostic Medical Device complying with EU Directive 98/79/EC.

This symbol, depending upon its location, indicates the assembly, component, and/or product are free of lead. In respect to its inclusion on a circuit component or assembly, the following must be adhered to when servicing. Failure to do so may result in defects caused by dissimilar metals.

1. Do not repair or modify using a lead-containing solder.

2. Do not repair or modify using any soldering tools that have been in contact with lead-containing solders.

3. Do not repair or modify with any lead-free solder other than that specified by the manufacturer or as indicated by the symbology of standard IPC-1066.

4. Do not repair or modify using any soldering tools that have been in contact with any other incompatible lead-free solders.

5. Contact factory service for further information.

The Advanced®Model 3250/4250 Service Manual

The number in this symbol indicates the type of lead-free solder used in the assembly of printed circuit boards, per standard IPC-1066. Repair or modification must be per-

XAMPLES

ONLY

formed using the same or compatible type lead-free solder and matching tools. Failure to do so may result in defects caused by dissimilar metals.

Contact factory service for further information.

FCC Requirements

• WARNING: Changes or modifications to this unit not expressly approved by Advanced Instruments could void the user’s authority to operate the equipment.

• This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against

harmful interference in a residential installation. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

- Re-orient or relocate the receiving antenna.

- Increase the separation between the equipment and receiver.

- Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.

- Consult the dealer or an experienced radio TV technician for help.

The Advanced®Model 3250/4250 Service Manual

Regulatory Description

Approval Type Applies to Serial Suffix A - C Applies to Serial Suffix D and Higher

U.S. Safety

Canadian Safety

CE Declaration of Conformity - EMC

CE Declaration of Conformity - IVD

(3250 only)

This product has been listed by ETL testing laboratories as being in compliance with the requirements of UL 61010A-1, 1st Edition, "Electrical Equipment for Laboratory Use; Part 1: General Requirements". The "US" in the lower right of the ETL mark demonstrates this listing.

This product has been listed by ETL testing laboratories as being in compliance with the requirements of CAN/CSA C22.2 No.1010.1-92, "Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use - Part 1: General Requirements"; Including Amendment Two. The "C" in the lower left of the ETL mark demonstrates this listing.

This product meets the intent of Directive 89/336/EEC for Electromagnetic Compatibility. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product for the latest update.

• EN 61326: 1997 with A1 & A2, Group 1, Class B, "Electrical Equipment for Measurement, Control, and Laboratory Use"

This product meets the intent of Directive 98/79/EC for In Vitro Diagnostic Medical Devices. Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

This product has been listed by ETL testing laboratories as being in compliance with the requirements of UL 61010-1, "Electrical Equipment for Laboratory Use; Part 1: General Requirements". The "US" in the lower right of the ETL mark demonstrates this listing.

This product has been listed by ETL testing laboratories as being in compliance with the requirements of CAN/CSA C22.2 No.61010.1, "Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use - Part 1: General Requirements"; Including Amendment Two. The "C" in the lower left of the ETL mark demonstrates this listing.

This product meets the intent of Directive 2004/108/EC Conformity - EMC for Electromagnetic Compatibility. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product for the latest update.

• EN 61326-1:2006 & EN 55011:1998, Group 1, Class B, "Electrical Equipment for Measurement, Control, and Laboratory Use"

CE Declaration of Conformity - WEEE

CE Declaration of Conformity - Low Voltage

(3250 only)

This product meets the intent of Directive 2002/96/EC as amended by 2003/108/EC for Waste Electrical and Electronic Equipment (WEEE). Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

This product meets the intent of Directive 73/23/EEC, the Low Voltage Directive. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

• EN 61010-1 (2001), “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 1: General Requirements”.

• EN 61010-2-101: 2002, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 2-101: Particular Requirements for In Vitro Diagnostic (IVD) Medical Equipment”.

This product meets the intent of Directive 2006/95/EC, the Low Voltage Directive. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

• EN 61010-1, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 1: General Requirements”.

• EN 61010-2-101, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 2-101: Particular Requirements for In Vitro Diagnostic (IVD) Medical Equipment”.

The Advanced®Model 3250/4250 Service Manual

Regulatory Description

Approval Type Applies to Serial Suffix A - C Applies to Serial Suffix D and Higher

FCC - Part 15 Subpart B, Class B

Canadian ICES-003

CB Report CB Certificate

U.S. FDA Listing

(3250 only)

Health Canada License

(3250 only)

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

This Class B digital apparatus complies with Canadian ICES-003

Cet appareil numérique de la classe B est conforme à la norme NMB-003 du Canada.

-------------------------------

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are listed with a U.S. Department of Health and Human Services, Food and Drug Administration, as:

Osmometer Class 1 Calibrators Class 2 Controls Class 1

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are licensed with Health Canada, Therapeutic Products Directorate, Medical Devices Bureau, as:

Osmometer Class 2 Calibrators Class 2 Controls Class 2

This Class B digital apparatus complies with Canadian ICES-003

Cet appareil numérique de la classe B est conforme à la norme NMB-003 du Canada.

A CB report and certificate have been issued for this product. The standard used was IEC 61010-1.

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are listed with a U.S. Department of Health and Human Services, Food and Drug Administration, as:

Osmometer Class 1 Calibrators Class 2 Controls Class 1

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are licensed with Health Canada, Therapeutic Products Directorate, Medical Devices Bureau, as:

Osmometer Class 2 Calibrators Class 2 Controls Class 2

CE Declaration of Conformity - RoHS

This product meets the intent of Directive 2002/95/EC for the Restriction of Use of Certain Hazardous Substances in Electrical and Electronic Equipment” as an exempt medical device per Article 2, Paragraph 1 and per Annex 1, Category 8 and/or 9 of Directive 2002/96/EC.

The Advanced®Model 3250/4250 Service Manual

Notes:

The Advanced®Model 3250/4250 Service Manual

2. General Overview

General Overview

(Intel Processor Systems)

Serial Suffix A - C

A. Head B. Motor C. Driver Transistors (Stir, Thermoelectric) D. Motor Capacitor E. Head Up/Head Down Relays F. Power Entry Module G. RS-232C Port H. Supervisor/Operator Keyswitch I. Barcode Port J. Power Supply K. Sample Probe Plugin

L. Block Probe Plugin M. Main Board Set, Application PCB N. Main Board Set, Processor PCB O. Printer P. Keypad Transition Board Q. Keypad R. Display S. Deck T. Heat Transfer Return Tube U. Heat Transfer Inlet Tube

The Advanced®Model 3250/4250 Service Manual

General Overview

(Intel Processor Systems)

Serial Suffix D and Higher

(underneath printer)

A. Head B. Motor C. Driver PCB D. Power Entry Module E. RS-232C Port F. Supervisor/Operator Keyswitch G. Barcode Port H. Power Supply (underneath printer) I. Sample Probe Plugin J. Block Probe Plugin

The Advanced®Model 3250/4250 Service Manual

K. Main Board Set, Processor PCB L. Printer M. Main Board Set, Application PCB N. Keypad Transition Board O. Keypad P. Display Q. Heat Transfer Return Tube R. Heat Transfer Inlet Tube S. Deck

Calibration

The 3250 has two ranges, each with its own calibration. To calibrate the low range, three freezing-point tests are required at the 100 mOsm/kg level, and six tests are required at the 1500 mOsm/kg level. To calibrate the high range, six freezing-point tests are required at both the 1500 mOsm/kg level and the 3000 mOsm/kg level.

The 4250 allows timed or plateau-seeking calibration in either the Hortvet (m°H) or the Celsius (m°C) scale. Six freezing-point tests are required at each of the 4250’s two calibration levels:

-408 m°C (-422 m°H), and

-600 m°C (-621 m°H).

Note: If you see the message “Recalibration

Needed”, follow the instrument prompts to complete the initial calibration. Your calibration may vary slightly from that mentioned here.

Calibrate the instrument according to the instructions below:

1. Turn the instrument on.

2. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position. It must remain there throughout calibration.

3. Press CALIB to begin the calibration sequence.

4. The display will prompt you at each calibration level. Place a sample of the first calibration standard into the freezing chamber and press START.

5. Continue loading and testing samples at the first calibration level until the display prompts you for samples at the second calibration level.

Refer to the instrument User’s Guide for additional information on calibration.

6. Place the sample of the second calibration standard into the freezing chamber and press START.

7. When the instrument has completed calibration, the display will read "Calibration Complete".

The Advanced®Model 3250/4250 Service Manual

3250/4250 Circuit Description

The instrument is comprised of the following functional blocks:

Power Supply

Serial Suffix A - C

The power entry assembly interfaces the AC voltage presented to the instrument with the transformer. The transformer provides 120 VAC to the power supply board and the head motor. The power supply provides the power to run the main printed circuit board (±12 VDC, ±5 VDC), the thermoelectrics (5 VDC), the fan (12 VDC), the stir/freeze coil (12 VDC), and the internal printer (5 VDC).

Note: The internal printer may be powered by

+5 VDC (red/orange wire) or +12 VDC (violet wire). Consult system schematics for proper service.

Power Supply

Serial Suffix D and Higher

VDC), the stir/freeze coil (12 VDC), the internal printer (12 VDC), and the motor and control circuits (+24 V).

Front End

The sample thermistor measures the temperature of the sample. The thermoelectrics (whose duty cycle is controlled through the block probe/thermistor) supercool the sample. The stir/freeze wire constantly stirs the sample to guarantee uniform cooling. When the supercooled sample reaches a set temperature (3000 mOsm for the low range and 4800 mOsm for the high range, by default, in the 3250, and 3000 mºC in the 4250), the stir/freeze wire "buzzes" causing the sample to freeze. During a test, the fan duty cycle tracks the thermoelectrics duty cycle -10%. If duty cycle drops below 20%, the fan is off. In between tests, the fan runs as required to maintain the software controlled target temperatures.

The power entry assembly interfaces the AC voltage presented to the instrument with the AC/DC switching power supply. The power supply provides the power to run the main printed circuit board set (±12 VDC, +5 VDC), the thermoelectrics (5 VDC), the fan (12

Barcode

Port

Serial Port

Head Motor

Controls

Processor

Board

Applications

Board

325605 Control Board Set Description

General: The control board set is made up of two printed circuit boards in a motherboard/ daughterboard configuration.

Power

Supply

Cooling

System

PrinterKeypadDisplay

The Advanced®Model 3250/4250 Service Manual

325620/425620/325621/425621 Processor Board

Processor: The processor used is an Intel 80C186 16-bit embedded processor. The 80C186 contains three programmable 16-bit timers, two serial ports, programmable interrupts, 1 mega-byte of memory address space, and 64 kilo-bytes of input/output (I/O) address space. The processor uses an external 32 MHz crystal to generate the internal 16 MHz system clock. The processor controls access to all memory and all I/O.

Memory Map: The 1 mega-byte of memory address space contains read-only memory (Flash EPROM), read/write memory (static RAM), and the real time clock. About half of the address space is unused, allowing for future expansion. The read-only memory is divided into four sections: reset vector, boot code, parameter blocks (unused), and applicaiton code. The reset vector tells the processor where to first start executing code; in this case, the boot code is executed first after reset. The boot code determines if new software will be downloaded by checking if the dip switch is in the “PROGRAM” position. If it is, the boot code waits for new software to be downloaded through the serial port. If not, the application code starts.

The real time clock maintains the current date and time, and contains nonvolatile memory where the instrument’s operation parameters are stored. A internal lithium battery preserves the contents of the nonvolatile memory when the instrument is turned off. Finally, the read-write memory is used for stacks, variables, etc., except for the small section allocated for the interrupt vectors. The interrupt vectors tell the processor what code to run when an interrupt occurs, and functions similarly to the reset vector.

Input/Output Map: The 64 kilo-bytes of I/O address space control access to all peripherals such as the serial ports, application board, etc. The processor uses 256 bytes, called the Peripheral Control Block, to control the integrated serial ports, timers, interrupts, chip selects, and I/O pins. The Peripheral Control Block is fully described in the 80C186EB/80C188EB Microprocessor User’s Manual. The application board’s registers and the CPU board’s registers control access to various functions and are described later.

Memory: Two types of memory are available: read-only and read/write. The chips used here have 8-bit wide data buses. Used in pairs, the memory becomes compatible with the processor’s 16-bit wide data bus. The read-only memory is implemented using two 128 kilo-bytes x 8 bits Flash EPROMs. Flash memory is only programmable when a programming voltage (in this case, 12 volts DC) is applied to the memory; otherwise, it behaves like read-only memory. The boot code controls the programming voltage, since this is the only place where new software can be downloaded. The read/write memory is implemented using two 128 kilo-bytes x 8 bits static RAMs.

Watchdog Timer: The watchdog timer provides a mechanism to reset the processor when the software is not behaving normally. The software “pets” the watchdog every 100 200 milliseconds. As long as the watchdog is “petted”, nothing happens. However, if the software stops “petting” the watchdog, the watchdog “bites” the processor by activating the processor’s RESET pin, causing the processor to reset. The watchdog will also reset the processor if the power supply falls below 4.75 VDC. These two features allow the instrument to recover from abnormal software and hardware conditions.

Please note that all memory addresses and sizes are always defined in terms of bytes, even though the processor handles words (1 word = 2 bytes, 1 byte = 8 bits; therefore, 16 bits = 1 word).

Real Time Clock (RTC): The real time

clock maintains the current date and time. The RTC uses an internal clock circuit with an internal, 10-year life, lithium battery to

The Advanced®Model 3250/4250 Service Manual

perform timekeeping. The battery also pre-

erves the contents of internal memory.

Dip Switch: The three-position or fourposition dip switch allows the user to download new software via one switch. The other two switches are available for selecting different operating modes. These operating modes and the process for downloading software are described later in this manual.

Voltages: Three voltages are present on the CPU board: +5 VDC, +12 VDC, and V

+5 VDC powers all the logic. +12 VDC is switched on and off to create the flash memory’s programming voltage, V

Glue Logic: The glue logic performs the functions of creating the RAM memory chip selects, the Flash memory write signals, the V

control signal, the watchdog control sig-

nal, and accessing the dip switch. The CPU board has two software accessible hardware registers to read the dip switch and to control watchdog and V

Connectors: A 64-pin connector provides address, data and interrupt signals. A 16-pin connector provides general chip selects and serial receive/transmit signals. The application PCB uses a subset of the available signals.

to a 1 mOsm/1.86mºC change. A separate

heatstone bridge circuit is used to measure

W the voltage across each thermistor probe. This voltage is first filtered and then sampled by the analog-to-digital converter. The analog-to-digital converter uses a sigma-delta conversion technique with on-chip filtering and a 6.25 VDC reference voltage.

I/O Ports: Circuitry for the two serial ports (RS-232 and barcode) and the parallel printer is present on the application board, with cabling to the actual connectors on the back panel and the internal printer. Display & Keypad: The display and keypad are interfaced to the processor via programmable logic devices (PLDs). The keypad is polled in a row/column process which identifies the key pressed to the microprocessor. The application logic, in response to processor commands, controls the LEDs located on the keypad.

Light Emitting Diodes (LED): LEDs are provided to indicate when a high current load is active, a sensor is tripped, or a voltage supply is active. Green LEDs are used on the power supply voltages, yellow LEDs are used on driver signals such as the fan and head motor controls, and red LEDs are used to indicate that the head up and down optical sensors have been tripped.

PCB605 Application Board

The application PCB contains all circuitry specific to the instrument. The board includes voltage supplies, indicators, analog-to-digital converters, drive circuitry, parallel ports, serial ports, and various switches.

Analog-to-Digital Converters: Two analogto-digital converters are provided; one for the sample thermistor probe, and one for the block thermistor probe. The thermistors vary in resistance from approximately 2 kilo-ohms at room temperature, to approximately 10 kilo-ohms at -12ºC. Typically, a 0.6 ohm change in the thermistor’s resistance equates

The Advanced®Model 3250/4250 Service Manual

Application Logic: The application logic is made up mainly of two programmable logic devices (PLDs). These provide software accessible hardware registers, enabling the software to read the keypad, sensors, and analog-to-digital converters. These also allow manipulation of the display, external components such as the fan, head motor, etc., and interface to the RS-232 ports. The printer is controlled directly from the microprocessor through an 8-pin D-latch.

Connectors: Connectors are supplied for the CPU PCB, display, keypad, power, drives, probes, and back panel I/O ports.

Serial (RS-232) Port Interface: An RS-232

ine driver/receiver provides the microproces-

l sor with a serial port interface that supports both hardware and software handshaking.

The DB-9 RS-232 port conforms to the DTE RS-232C standard and has the following pin assignments:

The stir wire coil is controlled by the rear

anel-mounted darlington transistor

p (PCB522). The drive circuit consists of a programmable timer, D/A converter, and interface op-amps. The timer provides a square wave of approximately 71Hz, while the D/A converter controls the output amplitude to the darlington transistor.

Signal Pin Direction

Carrier Detect 1 to 3250 Receive Data 2 to 3250 Transmit Data 3 from 3250 Data Terminal Ready 4 from 3250 Signal Ground 5 common Data Set Ready 6 to 3250 Request to Send 7 from 3250 Clear to Send 8 to 3250

Note that your instrument is only designed to support unidirectional communication with an external device. At this time, there is no protocol for bidirectional communication.

For a sample RS-232 Port Setup, please see the RS-232 Supplemental Information in the Appendix at the end of this user’s guide.

Note: This instrument requires the use of a

null modem RS-232C cable. There are several variations on null modem cables. Advanced Instruments recommends that you purchase a RS-232C cable direct from our factory.

Supervisor/Operator Keyswitch Interface:

A PLD is used to interface the supervisor/ operator keyswitch to the microprocessor. In Operator position, the user cannot change setup or calibration settings.

Other Circuit Components

Drive Circuitry, Serial Suffix A - C: Drive circuitry is provided to turn on and off the four high current loads such as the stir wire, the head motor, the thermoelectric cooler, and the fan.

The head motor is controlled by two relays located at the rear of the chassis that interface the 120 VAC motor to the DC logic. One relay raises the head, while the other relay lowers the head. The relays get their commands from the application board via two FETs and the application logic. LEDs are provided on the application board for monitoring the drive signals. LEDs are also provided to monitor the signals from the head sensor board, described below.

The thermoelectric cooler is controlled by the FET driver transistor mounted on the rear panel (PCB520). The FET is, in turn, controlled by the microprocessor through the PLD application logic. The processor varies the duty cycle square wave in response to software commands and block probe resistance.

Drive Circuitry, Serial Suffix D and Higher: Drive circuitry is provided to turn

on and off four system loads such as the stir wire, the head motor, the thermoelectric cooler, and the fan.

The stir wire coil drive circuit consists of a programmable timer, D/A converter, and interface op-amps on the application board (PCB605). The timer provides a square wave of approximately 71 Hz, while the D/A converter controls the output amplitude to the darlington transistor mounted on the driver board (PCB523).

The thermoelectric cooler is controlled by the microprocessor through the PLD application logic. The processor varies the duty cycle square wave in response to software com-

The Advanced®Model 3250/4250 Service Manual

mands and block probe resistance. These sig-

als are provided to the FET driver transistor

n mounted on the driver board (PCB523).

The head motor is controlled by the application board via two FETs and the PLD logic. LEDs are provided on the application board for monitoring the drive signals. LEDs are also provided to monitor the signals from the head sensor board, described below. Two active low head up/head down signals are sent to the driver board (PCB523) where they are converted into acceleration, direction, and step commands for the motor drive chip. The motor drive chip translates these commands into current controlled outputs that move the DC stepper motor and provide idle torque when no movement is required. The following adjustments may be available for the motor circuit: R10 and TP16 are used to set the initial acceleration to a rise time of 4uS. R21 and TP15 are used to set the run frequency to 8 Khz.

The fan is controlled by a FET transistor on the application board, that is interfaced to the processor through the application logic.

Head Transition Board (M20450) Description: The stir/freeze coil and the sample probe

are connected to the head transition board which allows easy sample probe replacement.

Head Sensor Board (3D3380) Description:

This board provides two optical sensors that interface to a PLD on the main board. These two sensors detect the head-up and head-down conditions.

Keypad Transition Board (FLO520) Description: This board provides the interface

to the keypad along with pull-up resistors for the keypad LEDs.

Barcode Port: A D-type, 15-pin barcode port is provided in the back of the instrument for connecting and providing power to such a device. For proper operation, the barcode port requires a 1200 bps, RS-232 signal providing asynchronous serial data containing 1 start bit, 8 data bits, 1 stop bit, and no parity.

Signal Pin Direction

+5V DC 1 to reader receive data 10 from reader gnd/earth 9 common

Barcode Port Connections

A suitable barcode scanner is available from Advanced Instruments. To interface with the instrument, the barcode scanner must be programmed as follows, referring to the scanner users guide.

1200 bps CR suffix disable beep after good decode triggerless trigger mode (optional)

Internal Printer, Serial Suffix A - C: The internal printer assembly (325400) consists of the sheet metal mounting bracket, thermal print head (FL0403), thermal printer PCB (FL0402), and thermal printer PC board assembly (PCB400). All of this is connected to the application board (PCB605) via cable (M20465).

Note: The internal printer may be powered by

+5 VDC (red/orange wire) or +12 VDC (violet wire). Consult system schematics for proper service.

Internal Printer, Serial D and Higher: The internal printer assembly (325420) consists of the sheet metal mounting bracket, thermal print head (325404), main control board (325421), printer interface board (PCB402), and the control cable (325419). All of this is connected to the application board (PCB605) via cable (325415), and on some models via cable (325033).

The printer interface board (PCB402) contains the power circuit converting +12 VDC into +5 VDC, a feed/self-test switch (S1), and some diagnostic LEDs. If the self-test switch is depressed as instrument power is applied, the printer will enter self-test mode and print out settings and a test character set. The LEDs indicate the operation of the paper-out detector (D1 yellow) and the door/platen-closed detector (D2 red). POWER CONNECTION TO J1

The Advanced®Model 3250/4250 Service Manual

SHOULD NOT BE RECONNECTED WITH INSTRUMENT POWER ON, OR DAMAGE TO THE INSTRUMENT MAY RESULT.

The printer control board (325421) translates the data from the application board (PDCB605) into control signals for the print head thermal elements and drive motor (325404). This board also contains a factory-set configuration DIP switch (DS1) and jumper (JP1).

On models equipped with cable (325033), a compatible version of main board set (325605)

board (PCB402), diagnostic LEDs, and a test switch are available in the lower right corner of the larger board (PCB605). D19 is a red LED that will indicate the printer door/platen interface is not closed or, if blinking, one of several printer errors have occurred, requiring factory assistance. D20 is a yellow LED that will indicate the paper is out or not detected when the door is closed. SW1 is a momentary switch that will cause the paper to advance when pressed or, if held down while main power is switched on, will place the printer into self-test mode.

and a properly configured printer interface

Design Changes

The serial number suffix (referred to in this manual simply as the suffix) indicates the revision of the instrument. The chart below lists the major changes made at each revision of the 3250 Osmometer and 4250 Cryoscope.

Model 3250

Osmometer Description

Serial Suffix ‘A’ Original model released. January 2004

Serial Suffix ‘B’ Base chassis and cover reMay 2007 designed.

Serial Suffix ‘C’ Up/Down relay PC boards reOctober 2007 designed into a single RoHS-

compliant PC board.

Serial Suffix ‘D’ **MAJOR REVISION** September 2009 AC head motor and supporting

components replaced with a DC stepper motor, multi-driver PC board and power supply, Seiko thermal printer assembly with thread-through paper loading and supporting components replaced with a Citizen thermal printer assembly with easy-load paper loading.

Serial Suffix ‘E’ Application board redesigned to October 2010 power display and keypad LEDs

from VCC +5 VDC line. Main harness redesigned.

Model 4250 Cryoscope Description

Serial Suffix ‘A’ Original model released. November 2005

Serial Suffix ‘B’ Base chassis and cover reMay 2007 designed.

Serial Suffix ‘C’ Up/Down relay PC boards reOctober 2007 designed into a single RoHS-

compliant PC board.

Serial Suffix ‘D’ **MAJOR REVISION** September 2009 AC head motor and supporting

Serial Suffix ‘E’ Application board redesigned to October 2010 power display and keypad LEDs

from VCC +5 VDC line. Main harness redesigned.

The Advanced®Model 3250/4250 Service Manual

Replacement Parts

Serial Suffix D and higher has some different replacement parts from Serial Suffix A - C, although many

parts have remained common. Before selecting a replacement part, check the serial suffix of the instrument

on the serial suffix label on the rear of the instrument, then refer to the proper section. Refer to the Design

Changes section for additional information.

3250

Common Parts

Block Probe . . . . . . . . . . . . . . . . . . . .4D3340

Clapper . . . . . . . . . . . . . . . . . . . . . .3C2241R

Control Board Set . . . . . . . . . . . . . .325605R

Application Board (only) . . . . . . . .PCB605R

Processor Board (only) . . . . . . . . . .325621R

Cooling Assembly . . . . . . . . . . . . . .3D3300R

Cooling Fan . . . . . . . . . . . . . . . . . . . .4D3360

Cooling Well Inlet Check Valve . . . . .99133R

Firmware “FLASH” Update Kit . . . .SFW008

Fluid Filter . . . . . . . . . . . . . . . . . . . .4D3710R

Fuses: 2.0 Amp . . . . . . . . . . . . . . . . . .70022

1.0 Amp . . . . . . . . . . . . . . . . . .70011

Heat Transfer Fluid Intake

Check Valve . . . . . . . . . . . . . . . .4D3705R

Heat Transfer Fluid Pump Assembly .4D3690

Keypad . . . . . . . . . . . . . . . . . . . . . . .325511R

Load Resistor . . . . . . . . . . . . . . . . . .425150R

Mandrel . . . . . . . . . . . . . . . . . . . . . . .3LH500

NVRAM Battery . . . . . . . . . . . . . . . .71027R

Probe Alignment Tool Kit . . . . . . . . .3LA700

Sample Probe . . . . . . . . . . . . . . . . . . .3D3700

Stir/Freeze Coil . . . . . . . . . . . . . . . .3D2404R

Stir/Freeze Wire . . . . . . . . . . . . . . . . .3LH243

Tube Ejector Spring . . . . . . . . . . . .3D3312R

Yoke . . . . . . . . . . . . . . . . . . . . . . . .3LH230R

4250

Common Parts

Block Probe . . . . . . . . . . . . . . . . . . . .4D3340

Clapper . . . . . . . . . . . . . . . . . . . . . .3C2241R

Control Board Set . . . . . . . . . . . . . .425605R

Application Board (only) . . . . . . . .PCB605R

Processor Board (only) . . . . . . . . . .425621R

Cooling Assembly . . . . . . . . . . . . . .4D3300R

Cooling Fan . . . . . . . . . . . . . . . . . . . .4D3360

Cooling Well Inlet Check Valve . . . . .99133R

Firmware “FLASH” Update Kit . . . .SFW008

Fluid Filter . . . . . . . . . . . . . . . . . . . .4D3710R

Fuses: 2.0 Amp . . . . . . . . . . . . . . . . . .70022

1.0 Amp . . . . . . . . . . . . . . . . . .70011

Heat Transfer Fluid Intake

Check Valve . . . . . . . . . . . . . . . .4D3705R

Heat Transfer Fluid Pump Assembly .4D3690

Keypad . . . . . . . . . . . . . . . . . . . . . . .325511R

Load Resistor . . . . . . . . . . . . . . . . . .425150R

Mandrel . . . . . . . . . . . . . . . . . . . . . . .3LH500

NVRAM Battery . . . . . . . . . . . . . . . .71027R

Probe Alignment Tool Kit . . . . . . . . .3LA700

Sample Probe . . . . . . . . . . . . . . . . . .4D3102

Stir/Freeze Coil . . . . . . . . . . . . . . . .3D2404R

Stir/Freeze Wire . . . . . . . . . . . . . . . . .4LH243

Tube Ejector Spring . . . . . . . . . . . . . .4D3312

Yoke . . . . . . . . . . . . . . . . . . . . . . . .3LH230R

Serial Suffix A - C Parts

Display Board . . . . . . . . . . . . . . . . . .74051R

Head Up/Head down Relay . . . . . . . .3D3109

Motor Assembly . . . . . . . . . . . . . . .4D3350R

Power Supply Assembly . . . . . . . . . .4D3950

Printer Assembly without

Power Harness . . . . . . . . . . . . . .FL2401R

Printer Assembly with Power

Harness . . . . . . . . . . . . . . . . . . . .325400R

Stir Drive Transistor . . . . . . . . . . . .PCB522R

Thermoelectric Drive Transistor . .PCB520R

The Advanced®Model 3250/4250 Service Manual

Serial Suffix A - C Parts

Display Board . . . . . . . . . . . . . . . . . .74051R

Head Up/Head Down Relay . . . . . . .3D3109

Motor Assembly . . . . . . . . . . . . . . .4D3350R

Power Supply Assembly . . . . . . . . . .4D3950

Printer Assembly without

Power Harness . . . . . . . . . . . . . .FL2401R

Printer Assembly with Power

Harness . . . . . . . . . . . . . . . . . . . .325400R

Stir Drive Transistor . . . . . . . . . . . .PCB522R

Thermoelectric Drive Transistor . .PCB520R

+ 165 hidden pages

Advanced 3250, 4250 User manual

Specifications and Main Features

Frequently Asked Questions

User Manual