Advanced 3250, 4250 User manual

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Advanced

Osmometer Model 3250

Cryoscope Model 4250

Service Manual

3255SM Rev22 020416

Table of Contents

1. Introduction

Specifications . . . . . . . . . . . . . . . . . . . . .7

General Description and Purpose . . . .11

Service Assistance . . . . . . . . . . . . . . . .11

Safety/Regulatory Information . . . . . .12

Symbol Conventions . . . . . . . . . . . . . .12

General Cautions . . . . . . . . . . . . . . . . .12

Service & Maintenance Cautions . . . .13

Hazardous Material Cautions . . . . . . .14

Regulatory Notices . . . . . . . . . . . . . . .14

Symbol Conventions . . . . . . . . . . . . . .14

FCC Requirements . . . . . . . . . . . . . . .15

2. General Overview

General Overview . . . . . . . . . . . . . . . .21

Calibration . . . . . . . . . . . . . . . . . . . . . .23

Circuit Description . . . . . . . . . . . . . . .24

Power Supply . . . . . . . . . . . . . . . . . . . .24

Front End . . . . . . . . . . . . . . . . . . . . . . .24

325605 Control Board Set . . . . . . . . . .24

325620/425620/325621/425621

Processor Board . . . . . . . . . . . . . . . .25

PCB605 Application Board . . . . . . . . .26

Other Circuit Components . . . . . . . . . .27

Design Changes . . . . . . . . . . . . . . . . . .29

Replacement Parts . . . . . . . . . . . . . . . .30

Supplies & Accessories . . . . . . . . . . . .32

3. Maintenance

Cleaning and Maintenance . . . . . . . . .35

Removing the Head & Instrument

Covers . . . . . . . . . . . . . . . . . . . . . . .36

Osmometer/Cryoscope Well Cleaning 37 Cleaning and Lubricating the Printer .39 Probe & Stir/Freeze Wire Alignment .40

4. Troubleshooting

Introduction . . . . . . . . . . . . . . . . . . . . .43

Freeze “Buzz” Amplitude . . . . . . . . . .44

Stir Driver Noise . . . . . . . . . . . . . . . . .45

Head Sensor Adjustment . . . . . . . . . . .47

Head Up/Down Test . . . . . . . . . . . . . .48

Power On Diagnostics . . . . . . . . . . . . .49

A/D Tests . . . . . . . . . . . . . . . . . . . . . . .49

Sample Bin Setting . . . . . . . . . . . . . . .50

Block Bin Setting . . . . . . . . . . . . . . . .51

Stir Amplitude . . . . . . . . . . . . . . . . . .51

Display/Printer Test . . . . . . . . . . . . . . .51

Beeper Test . . . . . . . . . . . . . . . . . . . . .52

Keypad Test . . . . . . . . . . . . . . . . . . . . .52

Barcode Test . . . . . . . . . . . . . . . . . . . .52

Reset Serial Number . . . . . . . . . . . . . .52

Instrument Software Updates . . . . . . .54

Performing FLASH firmware update .54

Troubleshooting Table . . . . . . . . . . . . .56

5. Replacement Instructions

Block Probe (4D3340) . . . . . . .325P340

Clapper (3C2241R) . . . . . . . .3C2P241R

Control Board Set (325605R/425605R),

Application Board (PCB605R), Processor Board (325621R/

425621R) . . . . . . . . . . . . . .325P620

Cooling Assembly (3D3300R/

4D3300R) . . . . . . . . . . . .325P300

Cooling Fan (4D3360) . . . . . . .325P360

Display Board (74051R/

702041R) . . . . . . . . . . . .325P051

Driver Board (PCB523R) . . .PCB523PM

Fuse (70011/70022) . . . . . . . . . . .325P11

Head Up Relay or Head Down

Relay (3D3109) . . . . . . . . . .325P109

Heat Transfer Fluid Pump

Assembly (4D3690), Fluid Filter (4D3710R), One-way Check

Valve (4D3705R/99133R) . . . .4DP690

Keypad (325511R) . . . . . . . . . .325P511

Load Resistor (425150R) . . . . .42P150R

Mandrel (3LH500) . . . . . . . . . .3LHP500

Motor Assembly (4D3350R/

325304R) . . . . . . . . . . . . . .325P350

NVRAM Battery (71027R) . . . .71P27R

Power Supply Assembly (4D3950/

325950R) . . . . . . . . . . . . . .325P950

Printer Assembly (325400R) . . .325P401 Printer Assembly (325420R) .325420PM Printer Mechanism

(325404R) . . . . . . . . . . . . . .325404PM

Probe Alignment Tool Kit

(3LA700) . . . . . . . . . . . . . . . . .3LP700

Sample Probe (3D3700) . . . . . . .3DP700

Sample Probe (3D3700) . . . . . .325P700

Sample Probe (4D3102) . . . . . . .4D3P02

Cryoscope Sample Probe

Versions FAQ . . . . . . . . . . . . .4DP002

Stir/Freeze Coil (3D2404R) . . .325P404

The Advanced®Model 3250/4250 Service Manual

Stir/Freeze Wire (3LH243/

4LH243) . . . . . . . . . . . . . . . .4LHP243

Transistor (PCB520R) . . . . . . .PCP520R

Transistor (PCB522R) . . . . . . .PCP522R

Tube Ejector Spring (3D3312R/

4D3312) . . . . . . . . . . . . . . . 4DP312

Upgrade Kit, 3250/4250 Suffix

D to E (325024) . . . . . . . . .325025PM

Yoke (3LH230R) . . . . . . . . . . .3LHP230

6. Schematics

PCB402 Assembly Drawing PCB402 Printer Interface Board

Schematic PCB402 Schematic PCB523 Assembly Drawing PCB523 Driver Board Schematic PCB523 Schematic PCB605 Assembly Drawing PCB605 Application Board Schematic PCB605 Schematic PCB620 Assembly Drawing PCB620 Processor Board Schematic 210102 Assembly Drawing 210102 Schematic 3D3380 Assembly Drawing 3D3380 Schematic 325000 Interconnection Schematic

APPENDICES

A: Symbol Definitions

B: Product Disposal and Recycling C: Service Log

Index

The Advanced®Model 3250/4250 Service Manual

1. Introduction

Model 3250 Osmometer Specifications

Applies to Serial Suffix A - C. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 130 VAC (1.2 A) or 200 to 250

VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 2-Amp time delay for 100-130 VAC;

1-Amp time delay for 200-250 VAC

Power: 150 Watts

Memory Backup: integral lithium cell; 10 years

min. (not user-replaceable)

Sample Volume: 0.2 or .25 mL

Sample Capacity: single sample

Readout: 20-character digital display

Units: mOsm/Kg H2O

Range:

Low: 0 to 2000 mOsm/Kg H2O High: 1400 to 4000 mOsm/Kg H2O

Resolution: 1 mOsm/Kg H2O

Performance Over Operating Conditions

Temperature Effects: Less than 1 mOsm/Kg H

for every 5°C (9°F) ambient temperature change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 2 minutes per sample

in 0 to 2000 mOsm range; moderately longer in high range.

Dimensions inches centimeters

Width: 13 32 Depth: 18 46 Height: 16 41

Communications:

RS-232C and barcode scanner ports

Weight pounds kilograms

Net: 28 13 Shipping: 39 19

Performance at Reference Conditions

Warranty: One-year limited warranty on work-

Linearity: Less than ±0.5% from a straight line

manship and all parts except glass, plastic and parts warranted by their

Repeatability:

makers.

0 to 400 mOsm ±2 mOsm/Kg (1 S.D.) 400 to 4000 mOsm ±0.5% (1 S.D.)

Drift: Less than 1 mOsm/Kg H

O per

Certification:

month

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I Over-Voltage Category: II Pollution Degree: 2 Moisture Protection: IPX0 (Ordinary)

Reference Conditions: 20 to 25°C (68 to 77°F); 40 to 60% Relative Humidity; tolerances of reference or calibration solutions

excluded.

The Advanced®Model 3250/4250 Service Manual

Model 3250 Osmometer Specifications

Applies to Serial Suffix D and Higher. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 250 VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 2-Amp time delay (Type T)

Power: 95 Watts

Memory Backup: integral lithium cell; 10 years

min. (not user-replaceable)

Sample Volume: 0.2 or .25 mL

Sample Capacity: single sample

Readout: 20-character digital display

Units: mOsm/Kg H2O

Range:

Low: 0 to 2000 mOsm/Kg H2O High: 1400 to 4000 mOsm/Kg H2O

Resolution: 1 mOsm/Kg H2O

Communications:

RS-232C and barcode scanner ports

Performance at Reference Conditions

Linearity: Less than ±0.5% from a straight line

Repeatability:

0 to 400 mOsm: Std. Deviation < 2 mOsm/Kg H2O 400 to 4000 mOsm: Std. Deviation < 0.5% of value

mOsm/Kg H2O

Drift: Less than 1 mOsm/Kg H

O per

month

Performance Over Operating Conditions

Temperature Effects: Less than 1 mOsm/Kg H

for every 5°C (9°F) ambient temperature change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 2 minutes per sample

in 0 to 2000 mOsm range; moderately longer in high range.

Dimensions inches centimeters

Width: 13 32 Depth: 18 46 Height: 16 41

Weight pounds kilograms

Net: 23 10 Shipping: 34 15

Warranty: One-year limited warranty on work-

manship and all parts except glass, plastic and parts warranted by their makers.

Certification:

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I Over-Voltage Category: II Pollution Degree: 2 Moisture Protection: IPX0 (Ordinary)

Reference Conditions: 20 to 25°C (68 to 77°F); 40 to 60% Relative Humidity; tolerances of reference or calibration solutions

excluded.

The Advanced®Model 3250/4250 Service Manual

Model 4250 Cryoscope Specifications

Applies to Serial Suffix A - C. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 130 VAC (1.2 A) or 200 to 250

VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 250V time delay (Type T):

2-Amp time delay for 100-130 VAC; 1-Amp time delay for 200-250 VAC

Power: 150 Watts

Memory Backup: integral lithium cell; 10-year

life (typical); (not userreplaceable)

Sample Volume: 2.0 to 2.5 mL

Sample Capacity: single sample

Readout: 20-character vacuum fluorescent

display

Units: mºC or mºH

Range: 0 to 1000 mºC or mºH

Resolution: 1 mºC or mºH

Communications: On-board printer, DTE EIA-

232/V.24 (RS-232) serial port and optional barcode scanner

Performance at Reference Conditions

Linearity: Less than ±0.5% from a straight line

Performance Over Operating Conditions

Temperature Effects: Less than 1 unit for every

5°C (9°F) ambient tempera ture change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 90 seconds in 30-

second timed mode.

Dimensions inches centimeters

Width: 13.0 33.0 Depth: 18.0 45.7 Height: 12.0 30.5

with Head Up: 16.0 40.6

Weight pounds kilograms

Net: 28.0 12.7 Shipping: 39.0 17.7

Warranty: One-year limited warranty on work-

manship and all parts except glass, plastic and parts warranted by their makers.

Repeatability:

± 2 mºC or mºH (1 S.D.) between 0 and -400 mºC ± 0.5% (1 S.D.) between -400 and -1000 mºC

Drift: Less than 1 unit per month

Certification:

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I

Over-Voltage Category: II

Pollution Degree: 2

Moisture Protection: IPX0 (ordinary)

The Advanced®Model 3250/4250 Service Manual

Model 4250 Cryoscope Specifications

Applies to Serial Suffix D and Higher. Refer to Design Changes for more information.

Electrical:

Voltage: 100 to 250 VAC (0.6 A)

Frequency: 50 or 60 Hz

Fuses: 2-Amp time delay (Type T)

Power: 95 Watts

Memory Backup: integral lithium cell; 10-year

life (typical); (not userreplaceable)

Sample Volume: 2.0 to 2.5 mL

Sample Capacity: single sample

Readout: 20-character vacuum fluorescent

display

Units: mºC or mºH

Range: 0 to 1000 mºC or mºH

Resolution: 1 mºC or mºH

Communications: On-board printer, DTE EIA-

232/V.24 (RS-232) serial port and optional barcode scanner

Performance at Reference Conditions

Performance Over Operating Conditions

Temperature Effects: Less than 1 unit for every

5°C (9°F) ambient tempera ture change.

Operating Conditions

Temperature: 18°C to 35°C (64°F to 95°F)

Room Humidity: 5 to 80% relative humidity;

(non-condensing)

Storage Temperature: -40°C to +45°C

(-40°F to +113°F)

Start-up Time: From stand-by: immediately

From power on: under 5 minutes

Test Time: Approximately 90 seconds in 30-

second timed mode.

Dimensions inches centimeters

Width: 13.0 33.0 Depth: 18.0 45.7 Height: 12.0 30.5

with Head Up: 16.0 40.6

Weight pounds kilograms

Net: 23 10 Shipping: 34 15

Linearity: Less than ±0.5% from a straight line

Repeatability:

0 to -400 mºC: Std. Deviation <

-400 to -1000 mºC: Std. Deviation < 0.5% of value mºC or mºH

Drift: Less than 1 unit per month

The Advanced®Model 3250/4250 Service Manual

2 mºC or mºH

Warranty: One-year limited warranty on work-

manship and all parts except glass, plastic and parts warranted by their makers.

Certification:

Refer to Regulatory Notices (see page 14) for applicable standards.

Installation Class: I

Over-Voltage Category: II

Pollution Degree: 2

Moisture Protection: IPX0 (ordinary)

General Description and Purpose

Service Assistance

Advanced

Osmometers and Cryoscopes

determine the concentration of solutions through freezing-point depression measurement. Osmometers are used primarily to measure body fluids such as serum and urine, and cryoscopes are used to determine the freezing points of various liquids and to detect added water in milk. Application information is available upon request.

Except for different cooling assemblies, stir/freeze wires, sample sizes and software, the 3250 and the 4250 are identical to one another. For clarity when replacing parts or troubleshooting, the instructions indicate any significant differences between the two instruments.

The Service Manual

This Service Manual contains:

To contact Advanced Instruments Product Service:

• Call (800) 225-4034 (toll-free within the USA and Canada; after normal business hours, dial extension 2191)

• Call +US (781) 320-9000 (elsewhere)

• Or fax (781) 320-3669

When calling Advanced Instruments, have the model and serial number from the label on the back of the instrument, and a description of the problem. Use a telephone as close to the instrument as possible in order to facilitate making recommended diagnostic checks.

A service technician may assist in making minor repairs over the phone, providing you with recommended parts (or part numbers), or may issue an authorization (RMA) to ship the instrument for factory repair.

• instructions for performing repairs.

• guides for troubleshooting operational and mechanical problems.

• circuit system illustrations.

• information about replacement parts and service repairs.

This manual is designed to assist service technicians, and does not imply a license to perform repairs without proper qualifications.

The RMA procedure is as follows:

1. Contact Advanced Instruments to obtain an RMA.

2. Pack and ship the instrument in its original carton. Do not send instrument supply items.

3. Prepay shipment to the factory. Advanced Instruments cannot accept collect ship-

ments. Insure the shipment or accept the damage risk.

The Advanced®Model 3250/4250 Service Manual

Safety / Regulatory Information

To reduce the risk of bodily injury, electric shock, fire, and damage to your instrument, please read and observe the following precautions.

• If the product is used in a manner not in accordance with the equipment design, operating instructions or manufacturer's recommendations, the operation of the product may be impaired to the extent that a safety hazard is created.

• Do not attempt to perform electrical work if you are not fully qualified. This manual is not a substitute for electrical training.

Symbol Conventions

The exclamation point within an equilateral triangle is intended to alert the user to the presence of important operating and maintenance (servicing) instructions in the literature accompanying this product.

The lightning flash with arrowhead symbol within an equilateral triangle is intended to alert the user to the presence of uninsulated dangerous voltage within the product's enclosure that may be of sufficient magnitude to constitute risk of electric shock to persons.

The static symbol within an equilateral triangle is intended to alert the user to the presence of internal components that could be damaged by static electricity.

This symbol indicates the presence of a fuse.

This symbol indicates the presence of protective earth ground.

This symbol indicates the power is ON.

This symbol indicates the power is OFF.

NOTE Additional symbol definitions are

available in the Symbols section.

General Cautions

• This product should be operated only with the type of power source indicated on the product’s electrical ratings label. Refer to the installation instructions included with the product.

• If the power cord provided is replaced for any reason or if an alternate cord is used, the cord must be approved for use in the local country. The power cord must be approved for the product’s listed operating voltage and be rated at least 20% greater than the ampere ratings marked on the product’s electrical ratings label. The cord end that connects to the product must have an IEC 60320 connector.

• Plug the product into an approved grounded electrical outlet.

This static symbol is intended to alert the user to the presence of a specific component that could be damaged by static electricity.

This symbol indicates the presence of alternating current (AC).

The Advanced®Model 3250/4250 Service Manual

• Do not disable the power cord’s grounding plug.

• If an extension cord or power strip is used, make sure that the cord or strip is rated for the product, and that the total ampere ratings of all products plugged into the extension cord or strip do not exceed 80% of the cord’s or strip’s rating limit.

• Route power cords so that they will not be walked on, tripped on, or pinched by items placed upon or against them. Pay particular attention to the plug, electrical outlet, and the point where the cord exits the product.

• Do not pull on cords and cables. When unplugging cords or cables, grasp the corresponding connector.

• Do not install or use this product in any area subject to extreme short-term temperature variations, or locations that exceed the specified operating environment temperatures.

• Never use this product in a wet area.

- The power cord, extension cord, power strip or power input module is damaged.

- Liquid has been spilled into the interior of the product.

- A foreign object has fallen into the product.

- The product has been dropped or damaged by a falling object.

- There are noticeable signs of overheating or a burning odor.

- The product does not operate normally when you follow the operating procedures.

• To avoid injury or fire hazard, do not operate this product in an explosive atmosphere.

• Do not install or use the product on an unstable, non-level work surface.

• Do not operate this product with the covers removed or unsecured.

Service & Maintenance Cautions

• Unplug the power cord prior to opening or removing covers, or else you may be exposed to electric shock, excessive temperatures, or mechanical hazards.

• Performing service or maintenance not detailed in the User’s Guide, with or without this Service Manual, should only be done by a qualified service technician.

• Never restrict airflow into or out of the product. Occasionally, check the air vents

for blockage.

• Wipe the exterior of the product with a

soft, damp cloth as needed. Using cleaning products other than those specified, may discolor or damage the finish.

• If the product requires service for any of

the following reasons, unplug the product from the electrical outlet and refer service to a qualified service technician.

- The main supply fuse(s) or any internal fuse(s) continually fail.

• A discharge of static electricity from contact with the human body or other conductor may damage system boards or static sensitive devices. Never perform internal maintenance without following recommended static protection procedures.

• The product is equipped with operator accessible fuses. If a fuse blows, it may be due to a power surge or failure of a component. Replace the fuse only once. If the fuse blows a second time, it is probably caused by failure of a component part. If this occurs, refer service to qualified service personnel. Always replace the fuse with one of the same rating, voltage, and type. Never replace the fuse with one of a higher current rating.

• When servicing the product, use only factory-specified parts.

• WARNING: When returning this product for service, or shipping this product to a second location, remove all hazardous specimens and decontaminate the product before packaging for shipment. If the product cannot be decontaminated, consult with your shipping agent on appropriate packaging and marking.

The Advanced®Model 3250/4250 Service Manual

Hazardous Material Cautions

• WARNING: Handle all biohazardous materials according to established good laboratory practices and follow your institution’s exposure control plan. Persons handling human blood and body fluid samples must be trained in blood-borne hazards and observe universal precautions. Universal precautions is an approach to infection control, where all human blood and body fluids are treated as if known to be infectious. Use personal protective equipment such as gloves, gowns, etc., to prevent exposure. Store biohazardous materials in regulated waste containers and dispose of these materials in a safe and acceptable manner that is in compliance with all country, state and local requirements.

• If a biohazardous material is spilled on or inside the equipment, decontaminate the equipment using a 1% bleach solution, or as outlined by those policies and procedures established within your institution.

• To avoid injury or fire hazard, do not operate this product in an explosive atmosphere.

Regulatory Notices

• This product has been designed and manu-

factured in accordance with U.S., Canadian, and European regulatory requirements as outlined below. Modifications made to this product that are not expressly approved in writing by the manufacturer will void the user’s authority to operate this product, previously issued factory approvals, and the user’s rights under the warranty.

• The distributor or dealer may have applied

additional local, national, or international approvals to this product. Consult the distributor or dealer for more information and documentation.

• Connections to this product must be made with shielded cables. Use of non-shielded cables may violate RFI/EMI limits.

Symbol Conventions

This symbol indicates conformity to relevant European directives.

This symbol indicates the product was tested to conform to relevant Canadian and U.S. safety standards by Intertek Testing Services NA, Inc. The ETL mark is approved in the United States as a Nationally Recognized Testing Lab (NRTL) by OSHA, and in Canada by the Standards Council of Canada.

In Vitro Diagnostic Medical Device complying with EU Directive 98/79/EC.

This symbol, depending upon its location, indicates the assembly, component, and/or product are free of lead. In respect to its inclusion on a circuit component or assembly, the following must be adhered to when servicing. Failure to do so may result in defects caused by dissimilar metals.

1. Do not repair or modify using a lead-containing solder.

2. Do not repair or modify using any soldering tools that have been in contact with lead-containing solders.

3. Do not repair or modify with any lead-free solder other than that specified by the manufacturer or as indicated by the symbology of standard IPC-1066.

4. Do not repair or modify using any soldering tools that have been in contact with any other incompatible lead-free solders.

5. Contact factory service for further information.

The Advanced®Model 3250/4250 Service Manual

The number in this symbol indicates the type of lead-free solder used in the assembly of printed circuit boards, per standard IPC-1066. Repair or modification must be per-

XAMPLES

ONLY

formed using the same or compatible type lead-free solder and matching tools. Failure to do so may result in defects caused by dissimilar metals.

Contact factory service for further information.

FCC Requirements

• WARNING: Changes or modifications to this unit not expressly approved by Advanced Instruments could void the user’s authority to operate the equipment.

• This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against

harmful interference in a residential installation. This equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instructions, may cause harmful interference to radio communications. However, there is no guarantee that interference will not occur in a particular installation. If this equipment does cause harmful interference to radio or television reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the interference by one or more of the following measures:

- Re-orient or relocate the receiving antenna.

- Increase the separation between the equipment and receiver.

- Connect the equipment into an outlet on a circuit different from that to which the receiver is connected.

- Consult the dealer or an experienced radio TV technician for help.

The Advanced®Model 3250/4250 Service Manual

Regulatory Description

Approval Type Applies to Serial Suffix A - C Applies to Serial Suffix D and Higher

U.S. Safety

Canadian Safety

CE Declaration of Conformity - EMC

CE Declaration of Conformity - IVD

(3250 only)

This product has been listed by ETL testing laboratories as being in compliance with the requirements of UL 61010A-1, 1st Edition, "Electrical Equipment for Laboratory Use; Part 1: General Requirements". The "US" in the lower right of the ETL mark demonstrates this listing.

This product has been listed by ETL testing laboratories as being in compliance with the requirements of CAN/CSA C22.2 No.1010.1-92, "Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use - Part 1: General Requirements"; Including Amendment Two. The "C" in the lower left of the ETL mark demonstrates this listing.

This product meets the intent of Directive 89/336/EEC for Electromagnetic Compatibility. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product for the latest update.

• EN 61326: 1997 with A1 & A2, Group 1, Class B, "Electrical Equipment for Measurement, Control, and Laboratory Use"

This product meets the intent of Directive 98/79/EC for In Vitro Diagnostic Medical Devices. Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

This product has been listed by ETL testing laboratories as being in compliance with the requirements of UL 61010-1, "Electrical Equipment for Laboratory Use; Part 1: General Requirements". The "US" in the lower right of the ETL mark demonstrates this listing.

This product has been listed by ETL testing laboratories as being in compliance with the requirements of CAN/CSA C22.2 No.61010.1, "Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use - Part 1: General Requirements"; Including Amendment Two. The "C" in the lower left of the ETL mark demonstrates this listing.

This product meets the intent of Directive 2004/108/EC Conformity - EMC for Electromagnetic Compatibility. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product for the latest update.

• EN 61326-1:2006 & EN 55011:1998, Group 1, Class B, "Electrical Equipment for Measurement, Control, and Laboratory Use"

CE Declaration of Conformity - WEEE

CE Declaration of Conformity - Low Voltage

(3250 only)

This product meets the intent of Directive 2002/96/EC as amended by 2003/108/EC for Waste Electrical and Electronic Equipment (WEEE). Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

This product meets the intent of Directive 73/23/EEC, the Low Voltage Directive. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

• EN 61010-1 (2001), “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 1: General Requirements”.

• EN 61010-2-101: 2002, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 2-101: Particular Requirements for In Vitro Diagnostic (IVD) Medical Equipment”.

This product meets the intent of Directive 2006/95/EC, the Low Voltage Directive. Compliance was demonstrated using the following standards, as listed in the Official Journal of the European Communities: Consult the Declaration of Conformance certificate shipped with the product (if required) for the latest update.

• EN 61010-1, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 1: General Requirements”.

• EN 61010-2-101, “Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use - Part 2-101: Particular Requirements for In Vitro Diagnostic (IVD) Medical Equipment”.

The Advanced®Model 3250/4250 Service Manual

Regulatory Description

Approval Type Applies to Serial Suffix A - C Applies to Serial Suffix D and Higher

FCC - Part 15 Subpart B, Class B

Canadian ICES-003

CB Report CB Certificate

U.S. FDA Listing

(3250 only)

Health Canada License

(3250 only)

This device complies with Part 15 of the FCC Rules. Operation is subject to the following two conditions: (1) this device may not cause harmful interference, and (2) this device must accept any interference received, including interference that may cause undesired operation.

This Class B digital apparatus complies with Canadian ICES-003

Cet appareil numérique de la classe B est conforme à la norme NMB-003 du Canada.

-------------------------------

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are listed with a U.S. Department of Health and Human Services, Food and Drug Administration, as:

Osmometer Class 1 Calibrators Class 2 Controls Class 1

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are licensed with Health Canada, Therapeutic Products Directorate, Medical Devices Bureau, as:

Osmometer Class 2 Calibrators Class 2 Controls Class 2

This Class B digital apparatus complies with Canadian ICES-003

Cet appareil numérique de la classe B est conforme à la norme NMB-003 du Canada.

A CB report and certificate have been issued for this product. The standard used was IEC 61010-1.

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are listed with a U.S. Department of Health and Human Services, Food and Drug Administration, as:

Osmometer Class 1 Calibrators Class 2 Controls Class 1

The osmometer, along with the calibrators and controls manufactured by Advanced Instruments, are licensed with Health Canada, Therapeutic Products Directorate, Medical Devices Bureau, as:

Osmometer Class 2 Calibrators Class 2 Controls Class 2

CE Declaration of Conformity - RoHS

This product meets the intent of Directive 2002/95/EC for the Restriction of Use of Certain Hazardous Substances in Electrical and Electronic Equipment” as an exempt medical device per Article 2, Paragraph 1 and per Annex 1, Category 8 and/or 9 of Directive 2002/96/EC.

The Advanced®Model 3250/4250 Service Manual

Notes:

The Advanced®Model 3250/4250 Service Manual

2. General Overview

General Overview

(Intel Processor Systems)

Serial Suffix A - C

A. Head B. Motor C. Driver Transistors (Stir, Thermoelectric) D. Motor Capacitor E. Head Up/Head Down Relays F. Power Entry Module G. RS-232C Port H. Supervisor/Operator Keyswitch I. Barcode Port J. Power Supply K. Sample Probe Plugin

L. Block Probe Plugin M. Main Board Set, Application PCB N. Main Board Set, Processor PCB O. Printer P. Keypad Transition Board Q. Keypad R. Display S. Deck T. Heat Transfer Return Tube U. Heat Transfer Inlet Tube

The Advanced®Model 3250/4250 Service Manual

General Overview

(Intel Processor Systems)

Serial Suffix D and Higher

(underneath printer)

A. Head B. Motor C. Driver PCB D. Power Entry Module E. RS-232C Port F. Supervisor/Operator Keyswitch G. Barcode Port H. Power Supply (underneath printer) I. Sample Probe Plugin J. Block Probe Plugin

The Advanced®Model 3250/4250 Service Manual

K. Main Board Set, Processor PCB L. Printer M. Main Board Set, Application PCB N. Keypad Transition Board O. Keypad P. Display Q. Heat Transfer Return Tube R. Heat Transfer Inlet Tube S. Deck

Calibration

The 3250 has two ranges, each with its own calibration. To calibrate the low range, three freezing-point tests are required at the 100 mOsm/kg level, and six tests are required at the 1500 mOsm/kg level. To calibrate the high range, six freezing-point tests are required at both the 1500 mOsm/kg level and the 3000 mOsm/kg level.

The 4250 allows timed or plateau-seeking calibration in either the Hortvet (m°H) or the Celsius (m°C) scale. Six freezing-point tests are required at each of the 4250’s two calibration levels:

-408 m°C (-422 m°H), and

-600 m°C (-621 m°H).

Note: If you see the message “Recalibration

Needed”, follow the instrument prompts to complete the initial calibration. Your calibration may vary slightly from that mentioned here.

Calibrate the instrument according to the instructions below:

1. Turn the instrument on.

2. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position. It must remain there throughout calibration.

3. Press CALIB to begin the calibration sequence.

4. The display will prompt you at each calibration level. Place a sample of the first calibration standard into the freezing chamber and press START.

5. Continue loading and testing samples at the first calibration level until the display prompts you for samples at the second calibration level.

Refer to the instrument User’s Guide for additional information on calibration.

6. Place the sample of the second calibration standard into the freezing chamber and press START.

7. When the instrument has completed calibration, the display will read "Calibration Complete".

The Advanced®Model 3250/4250 Service Manual

3250/4250 Circuit Description

The instrument is comprised of the following functional blocks:

Power Supply

Serial Suffix A - C

The power entry assembly interfaces the AC voltage presented to the instrument with the transformer. The transformer provides 120 VAC to the power supply board and the head motor. The power supply provides the power to run the main printed circuit board (±12 VDC, ±5 VDC), the thermoelectrics (5 VDC), the fan (12 VDC), the stir/freeze coil (12 VDC), and the internal printer (5 VDC).

Note: The internal printer may be powered by

+5 VDC (red/orange wire) or +12 VDC (violet wire). Consult system schematics for proper service.

Power Supply

Serial Suffix D and Higher

VDC), the stir/freeze coil (12 VDC), the internal printer (12 VDC), and the motor and control circuits (+24 V).

Front End

The sample thermistor measures the temperature of the sample. The thermoelectrics (whose duty cycle is controlled through the block probe/thermistor) supercool the sample. The stir/freeze wire constantly stirs the sample to guarantee uniform cooling. When the supercooled sample reaches a set temperature (3000 mOsm for the low range and 4800 mOsm for the high range, by default, in the 3250, and 3000 mºC in the 4250), the stir/freeze wire "buzzes" causing the sample to freeze. During a test, the fan duty cycle tracks the thermoelectrics duty cycle -10%. If duty cycle drops below 20%, the fan is off. In between tests, the fan runs as required to maintain the software controlled target temperatures.

The power entry assembly interfaces the AC voltage presented to the instrument with the AC/DC switching power supply. The power supply provides the power to run the main printed circuit board set (±12 VDC, +5 VDC), the thermoelectrics (5 VDC), the fan (12

Barcode

Port

Serial Port

Head Motor

Controls

Processor

Board

Applications

Board

325605 Control Board Set Description

General: The control board set is made up of two printed circuit boards in a motherboard/ daughterboard configuration.

Power

Supply

Cooling

System

PrinterKeypadDisplay

The Advanced®Model 3250/4250 Service Manual

325620/425620/325621/425621 Processor Board

Processor: The processor used is an Intel 80C186 16-bit embedded processor. The 80C186 contains three programmable 16-bit timers, two serial ports, programmable interrupts, 1 mega-byte of memory address space, and 64 kilo-bytes of input/output (I/O) address space. The processor uses an external 32 MHz crystal to generate the internal 16 MHz system clock. The processor controls access to all memory and all I/O.

Memory Map: The 1 mega-byte of memory address space contains read-only memory (Flash EPROM), read/write memory (static RAM), and the real time clock. About half of the address space is unused, allowing for future expansion. The read-only memory is divided into four sections: reset vector, boot code, parameter blocks (unused), and applicaiton code. The reset vector tells the processor where to first start executing code; in this case, the boot code is executed first after reset. The boot code determines if new software will be downloaded by checking if the dip switch is in the “PROGRAM” position. If it is, the boot code waits for new software to be downloaded through the serial port. If not, the application code starts.

The real time clock maintains the current date and time, and contains nonvolatile memory where the instrument’s operation parameters are stored. A internal lithium battery preserves the contents of the nonvolatile memory when the instrument is turned off. Finally, the read-write memory is used for stacks, variables, etc., except for the small section allocated for the interrupt vectors. The interrupt vectors tell the processor what code to run when an interrupt occurs, and functions similarly to the reset vector.

Input/Output Map: The 64 kilo-bytes of I/O address space control access to all peripherals such as the serial ports, application board, etc. The processor uses 256 bytes, called the Peripheral Control Block, to control the integrated serial ports, timers, interrupts, chip selects, and I/O pins. The Peripheral Control Block is fully described in the 80C186EB/80C188EB Microprocessor User’s Manual. The application board’s registers and the CPU board’s registers control access to various functions and are described later.

Memory: Two types of memory are available: read-only and read/write. The chips used here have 8-bit wide data buses. Used in pairs, the memory becomes compatible with the processor’s 16-bit wide data bus. The read-only memory is implemented using two 128 kilo-bytes x 8 bits Flash EPROMs. Flash memory is only programmable when a programming voltage (in this case, 12 volts DC) is applied to the memory; otherwise, it behaves like read-only memory. The boot code controls the programming voltage, since this is the only place where new software can be downloaded. The read/write memory is implemented using two 128 kilo-bytes x 8 bits static RAMs.

Watchdog Timer: The watchdog timer provides a mechanism to reset the processor when the software is not behaving normally. The software “pets” the watchdog every 100 200 milliseconds. As long as the watchdog is “petted”, nothing happens. However, if the software stops “petting” the watchdog, the watchdog “bites” the processor by activating the processor’s RESET pin, causing the processor to reset. The watchdog will also reset the processor if the power supply falls below 4.75 VDC. These two features allow the instrument to recover from abnormal software and hardware conditions.

Please note that all memory addresses and sizes are always defined in terms of bytes, even though the processor handles words (1 word = 2 bytes, 1 byte = 8 bits; therefore, 16 bits = 1 word).

Real Time Clock (RTC): The real time

clock maintains the current date and time. The RTC uses an internal clock circuit with an internal, 10-year life, lithium battery to

The Advanced®Model 3250/4250 Service Manual

perform timekeeping. The battery also pre-

erves the contents of internal memory.

Dip Switch: The three-position or fourposition dip switch allows the user to download new software via one switch. The other two switches are available for selecting different operating modes. These operating modes and the process for downloading software are described later in this manual.

Voltages: Three voltages are present on the CPU board: +5 VDC, +12 VDC, and V

+5 VDC powers all the logic. +12 VDC is switched on and off to create the flash memory’s programming voltage, V

Glue Logic: The glue logic performs the functions of creating the RAM memory chip selects, the Flash memory write signals, the V

control signal, the watchdog control sig-

nal, and accessing the dip switch. The CPU board has two software accessible hardware registers to read the dip switch and to control watchdog and V

Connectors: A 64-pin connector provides address, data and interrupt signals. A 16-pin connector provides general chip selects and serial receive/transmit signals. The application PCB uses a subset of the available signals.

to a 1 mOsm/1.86mºC change. A separate

heatstone bridge circuit is used to measure

W the voltage across each thermistor probe. This voltage is first filtered and then sampled by the analog-to-digital converter. The analog-to-digital converter uses a sigma-delta conversion technique with on-chip filtering and a 6.25 VDC reference voltage.

I/O Ports: Circuitry for the two serial ports (RS-232 and barcode) and the parallel printer is present on the application board, with cabling to the actual connectors on the back panel and the internal printer. Display & Keypad: The display and keypad are interfaced to the processor via programmable logic devices (PLDs). The keypad is polled in a row/column process which identifies the key pressed to the microprocessor. The application logic, in response to processor commands, controls the LEDs located on the keypad.

Light Emitting Diodes (LED): LEDs are provided to indicate when a high current load is active, a sensor is tripped, or a voltage supply is active. Green LEDs are used on the power supply voltages, yellow LEDs are used on driver signals such as the fan and head motor controls, and red LEDs are used to indicate that the head up and down optical sensors have been tripped.

PCB605 Application Board

The application PCB contains all circuitry specific to the instrument. The board includes voltage supplies, indicators, analog-to-digital converters, drive circuitry, parallel ports, serial ports, and various switches.

Analog-to-Digital Converters: Two analogto-digital converters are provided; one for the sample thermistor probe, and one for the block thermistor probe. The thermistors vary in resistance from approximately 2 kilo-ohms at room temperature, to approximately 10 kilo-ohms at -12ºC. Typically, a 0.6 ohm change in the thermistor’s resistance equates

The Advanced®Model 3250/4250 Service Manual

Application Logic: The application logic is made up mainly of two programmable logic devices (PLDs). These provide software accessible hardware registers, enabling the software to read the keypad, sensors, and analog-to-digital converters. These also allow manipulation of the display, external components such as the fan, head motor, etc., and interface to the RS-232 ports. The printer is controlled directly from the microprocessor through an 8-pin D-latch.

Connectors: Connectors are supplied for the CPU PCB, display, keypad, power, drives, probes, and back panel I/O ports.

Serial (RS-232) Port Interface: An RS-232

ine driver/receiver provides the microproces-

l sor with a serial port interface that supports both hardware and software handshaking.

The DB-9 RS-232 port conforms to the DTE RS-232C standard and has the following pin assignments:

The stir wire coil is controlled by the rear

anel-mounted darlington transistor

p (PCB522). The drive circuit consists of a programmable timer, D/A converter, and interface op-amps. The timer provides a square wave of approximately 71Hz, while the D/A converter controls the output amplitude to the darlington transistor.

Signal Pin Direction

Carrier Detect 1 to 3250 Receive Data 2 to 3250 Transmit Data 3 from 3250 Data Terminal Ready 4 from 3250 Signal Ground 5 common Data Set Ready 6 to 3250 Request to Send 7 from 3250 Clear to Send 8 to 3250

Note that your instrument is only designed to support unidirectional communication with an external device. At this time, there is no protocol for bidirectional communication.

For a sample RS-232 Port Setup, please see the RS-232 Supplemental Information in the Appendix at the end of this user’s guide.

Note: This instrument requires the use of a

null modem RS-232C cable. There are several variations on null modem cables. Advanced Instruments recommends that you purchase a RS-232C cable direct from our factory.

Supervisor/Operator Keyswitch Interface:

A PLD is used to interface the supervisor/ operator keyswitch to the microprocessor. In Operator position, the user cannot change setup or calibration settings.

Other Circuit Components

Drive Circuitry, Serial Suffix A - C: Drive circuitry is provided to turn on and off the four high current loads such as the stir wire, the head motor, the thermoelectric cooler, and the fan.

The head motor is controlled by two relays located at the rear of the chassis that interface the 120 VAC motor to the DC logic. One relay raises the head, while the other relay lowers the head. The relays get their commands from the application board via two FETs and the application logic. LEDs are provided on the application board for monitoring the drive signals. LEDs are also provided to monitor the signals from the head sensor board, described below.

The thermoelectric cooler is controlled by the FET driver transistor mounted on the rear panel (PCB520). The FET is, in turn, controlled by the microprocessor through the PLD application logic. The processor varies the duty cycle square wave in response to software commands and block probe resistance.

Drive Circuitry, Serial Suffix D and Higher: Drive circuitry is provided to turn

on and off four system loads such as the stir wire, the head motor, the thermoelectric cooler, and the fan.

The stir wire coil drive circuit consists of a programmable timer, D/A converter, and interface op-amps on the application board (PCB605). The timer provides a square wave of approximately 71 Hz, while the D/A converter controls the output amplitude to the darlington transistor mounted on the driver board (PCB523).

The thermoelectric cooler is controlled by the microprocessor through the PLD application logic. The processor varies the duty cycle square wave in response to software com-

The Advanced®Model 3250/4250 Service Manual

mands and block probe resistance. These sig-

als are provided to the FET driver transistor

n mounted on the driver board (PCB523).

The head motor is controlled by the application board via two FETs and the PLD logic. LEDs are provided on the application board for monitoring the drive signals. LEDs are also provided to monitor the signals from the head sensor board, described below. Two active low head up/head down signals are sent to the driver board (PCB523) where they are converted into acceleration, direction, and step commands for the motor drive chip. The motor drive chip translates these commands into current controlled outputs that move the DC stepper motor and provide idle torque when no movement is required. The following adjustments may be available for the motor circuit: R10 and TP16 are used to set the initial acceleration to a rise time of 4uS. R21 and TP15 are used to set the run frequency to 8 Khz.

The fan is controlled by a FET transistor on the application board, that is interfaced to the processor through the application logic.

Head Transition Board (M20450) Description: The stir/freeze coil and the sample probe

are connected to the head transition board which allows easy sample probe replacement.

Head Sensor Board (3D3380) Description:

This board provides two optical sensors that interface to a PLD on the main board. These two sensors detect the head-up and head-down conditions.

Keypad Transition Board (FLO520) Description: This board provides the interface

to the keypad along with pull-up resistors for the keypad LEDs.

Barcode Port: A D-type, 15-pin barcode port is provided in the back of the instrument for connecting and providing power to such a device. For proper operation, the barcode port requires a 1200 bps, RS-232 signal providing asynchronous serial data containing 1 start bit, 8 data bits, 1 stop bit, and no parity.

Signal Pin Direction

+5V DC 1 to reader receive data 10 from reader gnd/earth 9 common

Barcode Port Connections

A suitable barcode scanner is available from Advanced Instruments. To interface with the instrument, the barcode scanner must be programmed as follows, referring to the scanner users guide.

1200 bps CR suffix disable beep after good decode triggerless trigger mode (optional)

Internal Printer, Serial Suffix A - C: The internal printer assembly (325400) consists of the sheet metal mounting bracket, thermal print head (FL0403), thermal printer PCB (FL0402), and thermal printer PC board assembly (PCB400). All of this is connected to the application board (PCB605) via cable (M20465).

Note: The internal printer may be powered by

+5 VDC (red/orange wire) or +12 VDC (violet wire). Consult system schematics for proper service.

Internal Printer, Serial D and Higher: The internal printer assembly (325420) consists of the sheet metal mounting bracket, thermal print head (325404), main control board (325421), printer interface board (PCB402), and the control cable (325419). All of this is connected to the application board (PCB605) via cable (325415), and on some models via cable (325033).

The printer interface board (PCB402) contains the power circuit converting +12 VDC into +5 VDC, a feed/self-test switch (S1), and some diagnostic LEDs. If the self-test switch is depressed as instrument power is applied, the printer will enter self-test mode and print out settings and a test character set. The LEDs indicate the operation of the paper-out detector (D1 yellow) and the door/platen-closed detector (D2 red). POWER CONNECTION TO J1

The Advanced®Model 3250/4250 Service Manual

SHOULD NOT BE RECONNECTED WITH INSTRUMENT POWER ON, OR DAMAGE TO THE INSTRUMENT MAY RESULT.

The printer control board (325421) translates the data from the application board (PDCB605) into control signals for the print head thermal elements and drive motor (325404). This board also contains a factory-set configuration DIP switch (DS1) and jumper (JP1).

On models equipped with cable (325033), a compatible version of main board set (325605)

board (PCB402), diagnostic LEDs, and a test switch are available in the lower right corner of the larger board (PCB605). D19 is a red LED that will indicate the printer door/platen interface is not closed or, if blinking, one of several printer errors have occurred, requiring factory assistance. D20 is a yellow LED that will indicate the paper is out or not detected when the door is closed. SW1 is a momentary switch that will cause the paper to advance when pressed or, if held down while main power is switched on, will place the printer into self-test mode.

and a properly configured printer interface

Design Changes

The serial number suffix (referred to in this manual simply as the suffix) indicates the revision of the instrument. The chart below lists the major changes made at each revision of the 3250 Osmometer and 4250 Cryoscope.

Model 3250

Osmometer Description

Serial Suffix ‘A’ Original model released. January 2004

Serial Suffix ‘B’ Base chassis and cover reMay 2007 designed.

Serial Suffix ‘C’ Up/Down relay PC boards reOctober 2007 designed into a single RoHS-

compliant PC board.

Serial Suffix ‘D’ **MAJOR REVISION** September 2009 AC head motor and supporting

components replaced with a DC stepper motor, multi-driver PC board and power supply, Seiko thermal printer assembly with thread-through paper loading and supporting components replaced with a Citizen thermal printer assembly with easy-load paper loading.

Serial Suffix ‘E’ Application board redesigned to October 2010 power display and keypad LEDs

from VCC +5 VDC line. Main harness redesigned.

Model 4250 Cryoscope Description

Serial Suffix ‘A’ Original model released. November 2005

Serial Suffix ‘B’ Base chassis and cover reMay 2007 designed.

Serial Suffix ‘C’ Up/Down relay PC boards reOctober 2007 designed into a single RoHS-

compliant PC board.

Serial Suffix ‘D’ **MAJOR REVISION** September 2009 AC head motor and supporting

Serial Suffix ‘E’ Application board redesigned to October 2010 power display and keypad LEDs

from VCC +5 VDC line. Main harness redesigned.

The Advanced®Model 3250/4250 Service Manual

Replacement Parts

Serial Suffix D and higher has some different replacement parts from Serial Suffix A - C, although many

parts have remained common. Before selecting a replacement part, check the serial suffix of the instrument

on the serial suffix label on the rear of the instrument, then refer to the proper section. Refer to the Design

Changes section for additional information.

3250

Common Parts

Block Probe . . . . . . . . . . . . . . . . . . . .4D3340

Clapper . . . . . . . . . . . . . . . . . . . . . .3C2241R

Control Board Set . . . . . . . . . . . . . .325605R

Application Board (only) . . . . . . . .PCB605R

Processor Board (only) . . . . . . . . . .325621R

Cooling Assembly . . . . . . . . . . . . . .3D3300R

Cooling Fan . . . . . . . . . . . . . . . . . . . .4D3360

Cooling Well Inlet Check Valve . . . . .99133R

Firmware “FLASH” Update Kit . . . .SFW008

Fluid Filter . . . . . . . . . . . . . . . . . . . .4D3710R

Fuses: 2.0 Amp . . . . . . . . . . . . . . . . . .70022

1.0 Amp . . . . . . . . . . . . . . . . . .70011

Heat Transfer Fluid Intake

Check Valve . . . . . . . . . . . . . . . .4D3705R

Heat Transfer Fluid Pump Assembly .4D3690

Keypad . . . . . . . . . . . . . . . . . . . . . . .325511R

Load Resistor . . . . . . . . . . . . . . . . . .425150R

Mandrel . . . . . . . . . . . . . . . . . . . . . . .3LH500

NVRAM Battery . . . . . . . . . . . . . . . .71027R

Probe Alignment Tool Kit . . . . . . . . .3LA700

Sample Probe . . . . . . . . . . . . . . . . . . .3D3700

Stir/Freeze Coil . . . . . . . . . . . . . . . .3D2404R

Stir/Freeze Wire . . . . . . . . . . . . . . . . .3LH243

Tube Ejector Spring . . . . . . . . . . . .3D3312R

Yoke . . . . . . . . . . . . . . . . . . . . . . . .3LH230R

4250

Common Parts

Block Probe . . . . . . . . . . . . . . . . . . . .4D3340

Clapper . . . . . . . . . . . . . . . . . . . . . .3C2241R

Control Board Set . . . . . . . . . . . . . .425605R

Application Board (only) . . . . . . . .PCB605R

Processor Board (only) . . . . . . . . . .425621R

Cooling Assembly . . . . . . . . . . . . . .4D3300R

Cooling Fan . . . . . . . . . . . . . . . . . . . .4D3360

Cooling Well Inlet Check Valve . . . . .99133R

Firmware “FLASH” Update Kit . . . .SFW008

Fluid Filter . . . . . . . . . . . . . . . . . . . .4D3710R

Fuses: 2.0 Amp . . . . . . . . . . . . . . . . . .70022

1.0 Amp . . . . . . . . . . . . . . . . . .70011

Heat Transfer Fluid Intake

Check Valve . . . . . . . . . . . . . . . .4D3705R

Heat Transfer Fluid Pump Assembly .4D3690

Keypad . . . . . . . . . . . . . . . . . . . . . . .325511R

Load Resistor . . . . . . . . . . . . . . . . . .425150R

Mandrel . . . . . . . . . . . . . . . . . . . . . . .3LH500

NVRAM Battery . . . . . . . . . . . . . . . .71027R

Probe Alignment Tool Kit . . . . . . . . .3LA700

Sample Probe . . . . . . . . . . . . . . . . . .4D3102

Stir/Freeze Coil . . . . . . . . . . . . . . . .3D2404R

Stir/Freeze Wire . . . . . . . . . . . . . . . . .4LH243

Tube Ejector Spring . . . . . . . . . . . . . .4D3312

Yoke . . . . . . . . . . . . . . . . . . . . . . . .3LH230R

Serial Suffix A - C Parts

Display Board . . . . . . . . . . . . . . . . . .74051R

Head Up/Head down Relay . . . . . . . .3D3109

Motor Assembly . . . . . . . . . . . . . . .4D3350R

Power Supply Assembly . . . . . . . . . .4D3950

Printer Assembly without

Power Harness . . . . . . . . . . . . . .FL2401R

Printer Assembly with Power

Harness . . . . . . . . . . . . . . . . . . . .325400R

Stir Drive Transistor . . . . . . . . . . . .PCB522R

Thermoelectric Drive Transistor . .PCB520R

The Advanced®Model 3250/4250 Service Manual

Serial Suffix A - C Parts

Display Board . . . . . . . . . . . . . . . . . .74051R

Head Up/Head Down Relay . . . . . . .3D3109

Motor Assembly . . . . . . . . . . . . . . .4D3350R

Power Supply Assembly . . . . . . . . . .4D3950

Printer Assembly without

Power Harness . . . . . . . . . . . . . .FL2401R

Printer Assembly with Power

Harness . . . . . . . . . . . . . . . . . . . .325400R

Stir Drive Transistor . . . . . . . . . . . .PCB522R

Thermoelectric Drive Transistor . .PCB520R

3250

4250

Serial Suffix D and Higher Parts

Display Board . . . . . . . . . . . . . . . . .702041R

Driver PCB . . . . . . . . . . . . . . . . . . .PCB523R

Motor Assembly . . . . . . . . . . . . . . . .325304R

Power Supply Assembly . . . . . . . . .325950R

Thermal Printer Assembly . . . . . . . .325420R

Thermal Printer Mechanism . . . . . .325404R

Upgrade Kit, 3250/4250 Suffix . . . . .325024

D to E

Serial Suffix D and Higher Parts

Display Board . . . . . . . . . . . . . . . . .702041R

Driver PCB . . . . . . . . . . . . . . . . . . .PCB523R

Motor Assembly . . . . . . . . . . . . . . . .325304R

Power Supply Assembly . . . . . . . . .325950R

Thermal Printer Assembly . . . . . . . .325420R

Thermal Printer Mechanism . . . . . .325404R

Upgrade Kit, 3250/4250 Suffix . . . . .325024

D to E

The Advanced®Model 3250/4250 Service Manual

Supplies & Accessories

3250

100 mOsm/kg Calibration Standard

(10 5-mL ampules) . . . . . . . . . . . .3LA011

500 mOsm/kg Calibration Standard

(10 5-mL ampules) . . . . . . . . . . . .3LA051

900 mOsm/kg Calibration Standard

(10 5-mL ampules) . . . . . . . . . . . .3LA091

1500 mOsm/kg Calibration Standard

(10 5-mL ampules) . . . . . . . . . . . .3LA151

2000 mOsm/kg Calibration Standard

(10 5-mL ampules) . . . . . . . . . . . .3LA201

3000 mOsm/kg Calibration Standard

(10 5-mL ampules) . . . . . . . . . . . .3LA301

100 mOsm/kg Calibration Standard

(110-mL bottle) . . . . . . . . . . . . . .3LA010

500 mOsm/kg Calibration Standard

(110-mL bottle) . . . . . . . . . . . . . .3LA050

900 mOsm/kg Calibration Standard

(110-mL bottle) . . . . . . . . . . . . . .3LA090

1500 mOsm/kg Calibration Standard

(110-mL bottle) . . . . . . . . . . . . . .3LA150

Clinitrol™ 290 Reference Solution

(10 5-mL ampules) . . . . . . . . . . . .3LA029

Osmolality Linearity Set (5 levels) . .3LA028 Protinol® Near Serum Reference

(3 levels) . . . . . . . . . . . . . . . . . . .3MA028

Renol™ Urine Osmolality Controls

(2 levels) . . . . . . . . . . . . . . . . . . . .3LA085

Air Filters, disposable . . . . . . . . . . . .3D2340

Barcode Scanner . . . . . . . . . . . . . . . . .330016

Heat Transfer Fluid . . . . . . . . . . . . . .3DA811

Power Cord (specify voltage and

country) . . . . . . . . . . . . . . . . . . . .----------

Printer Maintenance Kit . . . . . . . . . .FL0425*

Printer Paper, pkg rolls . . . . . . . . . . .FLA835

Printer Paper Roll Holder . . . . . . . . . .FL0408

Sample Tubes (0.2 mL, disposable) .3LA825

Sample Tube Rack . . . . . . . . . . . . . . .3LA846

Serial Port Computer Cable

with DB9S . . . . . . . . . . . .RS232-CABLE

Supervisor Keys . . . . . . . . . . . . . . . . .3D3185

Service Manual . . . . . . . . . . . . . . . . .3255SM

User’s Guide . . . . . . . . . . . . . . . . . . . . . .3255

4250

-422 m°H Calibration Standard

(-408 m°C) . . . . . . . . . . . . . . . . . .3LA023

-621 m°H Calibration Standard

(-600 m°C) . . . . . . . . . . . . . . . . . .3LA033

-530 m°H Lactrol® 530 Reference

Solution (-512 m°C) . . . . . . . . . .3LA030

Air Filters, Disposable . . . . . . . . . . . .3D2340

Barcode Scanner . . . . . . . . . . . . . . . . .330016

Heat Transfer Fluid . . . . . . . . . . . . . .3DA811

Power Cord (specify voltage and

country) . . . . . . . . . . . . . . . . . . . .----------

Printer Maintenance Kit . . . . . . . . . .FL0425*

Printer Paper (5 rolls) . . . . . . . . . . . .FLA835

Printer Paper Roll Holder . . . . . . . . . .FL0408

Sample Tubes marked at 2-mL

and 2.5-mL . . . . . . . . . . . . . . . . . .3LA823

Sample Tube Rack . . . . . . . . . . . . . . .3LA846

Serial Port Computer Cable

with DB9S . . . . . . . . . . . .RS232-CABLE

Service Manual . . . . . . . . . . . . . . . . .3255SM

Supervisor Keys . . . . . . . . . . . . . . . . .3D3185

User’s Guide . . . . . . . . . . . . . . . . . . . . . .4255

*Serial Suffix A - C, only.

How to order:

To order parts, supplies and accessories, contact the Advanced Instruments Customer Service.

• 800-225-4034 (toll-free within the USA

and Canada)

• +US 781-320-9000 (elsewhere)

• +US 781-320-3669 (fax)

The Advanced®Model 3250/4250 Service Manual

3. Maintenance

Cleaning and Maintenance

The cooling assembly should be cleaned once every two months to insure optimal performance.

To flush the system:

1. Open the door and remove the heat transfer fluid bottle.

2. Empty the contents and fill the bottle halfway with a solution of 1:5 bleach in distilled water.

3. Replace the bottle and insert only the tube with the filter (A). Completely immerse the filter in the fluid.

4. Insert the return tube (B) into another container.

5. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position.

6. Press TEST to enter the test menu. Press < or > to select "Head Up Down Test" and press START.

7. Insert an empty sample tube into the freezing chamber and press START.

8. Run this test for 10-15 minutes and then press STOP.

Note: Using the manual primer pump to

complement the head pumping action can help reduce the time required to flush the system.

9. Remove the bottle with the diluted bleach and install a new bottle of heat transfer fluid. Insert only the tube with the filter (A).

10. Press START twice. Run this test until the colored fluid begins draining out of the return tube, and then press STOP.

11. Remove the return tube from the container and insert it into the heat transfer fluid bottle. Do not immerse the return tube in the heat transfer fluid (B).

12 Press STOP to exit the menu.

To drain the system:

1. Turn off the power and unplug the instrument. Remove the cover.

2. Remove the heat transfer fluid bottle and empty the contents.

3. Replace the empty bottle and insert the tubes.

4. Press gently on the pump at the bottom of the head. Slide until all liquid has been expelled (the unit may need to be tipped on its side to remove the liquid).

The Advanced®Model 3250/4250 Service Manual

Removing the Head Cover & the Instrument Cover

Tools Needed: Flat-bladed screwdriver,

Phillips screwdriver.

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper connections may cause damage to the instrument.

1. Turn off the power and unplug the instrument.

2. Remove the two screws from the head cover (A) and lift off the head cover.

3. Serial Suffix A: Remove the two screws from the front (B) and the four screws from the rear panel (C).

Serial Suffix B & C: Remove the four screws from the rear panel (C).

Serial Suffix D and Higher: Press the printer cover release button (D), then remove the four screws from the rear panel (C).

4. Serial Suffix A: Slide the instrument cover up and off.

Serial Suffix B and Higher: Slide the instrument cover forward, then up and off.

The Advanced®Model 3250/4250 Service Manual

Osmometer/Cryoscope Well Cleaning

Use this procedure to clean the well and tubing of any foreign matter (e.g., broken glass) that may have fallen into the well.

Tools needed: Flat-bladed screwdriver; Phillips screwdriver; Tru-arc pliers; thin, stiff wire; 4” cable tie; small wire cutters.

Osmometer Well Cleaning

1. Turn off the power and unplug the instru-

ment.

2. Remove the head cover and instrument

cover (see Removing the Head Cover & the Instrument Cover).

3. Drain the cooling system (see Cleaning

and Maintenance).

4. Disconnect the fluid pump from the cool-

ing well, as follows:

6. Remove the white Delrin elevator and spring (C). (Save for replacement when cleaning is complete.)

7. Inspect the bottom of the well and remove any visible debris. Be sure to clean out the small hole at the bottom of the well.

a. Unwrap refrigeration tape (A). Save

for re-use during reassembly.

b. Cut and remove the cable tie.

c. Pull the tubing off of the cooling well

inlet port.

5. Using a pair of Tru-arc pliers, remove the Tru-arc retainer ring (B) from inside the well. (Save the retainer ring for replacement when cleaning is complete.)

8. Using a thin stiff wire, insert it into the well inlet port.

Note: The wire diameter MUST be small

enough to pass through, into the well. The .050” diameter X 4.00” long 4LH243 stir wire is ideal.

9. Push the wire into the well. Repeat this process several times, sliding the wire back and forth until the end of the wire is visible in the hole at the bottom of the well. (This should loosen any deposit trapped in the tube and the well.)

10. Reassemble the pump tubing to the well, apply the cable tie, and then reapply the refrigeration tape.

11. Using a solution of 1:5 bleach in distilled water, place the filter and well return line into this solution. Manually press the pump up and down until the solution has

The Advanced®Model 3250/4250 Service Manual

circulated through the pump and well,

nd into the bottle.

12. When satisfied that the solution is freeflowing, flush the bleach solution with clean water, then stop and drain the cooling system.

13. Reassemble the instrument by replacing the spring (C) and elevator (B), securing with the Tru-arc retaining ring, then replace the head cover and instrument cover.

14. Add a new bottle of heat transfer fluid and prime the pump. The instrument is ready for testing.

Cryoscope Well Cleaning

1. Turn off the power and unplug the instrument. Remove the heat transfer fluid from the front cover.

2. Follow steps 2-4 from the Osmometer Well cleaning instruction (previous page).

3. Remove the spring (D) from the well and set aside, to be replaced after cleaning.

Note the orientation of the spring as you remove it from the well, wide-end down.

4. Follow steps 7-12 from the Osmometer Well Cleaning instruction.

5. Replace the spring (wide-end down) into the well and reassemble the instrument by replacing the instrument cover and the head cover.

6. Add a new bottle of heat transfer fluid and prime the pump. The instrument is ready for testing.

The Advanced®Model 3250/4250 Service Manual

Cleaning and Lubricating the Printer

(Serial Suffix A - C, only)

Use this procedure to clean and lubricate the printer feed mechanism.

Tools needed: Nyogel 744 lubricant and cleaning patches (Printer Maintenance Kit FL0425).

1. Open the printer cover on the top of the instrument. There is no need to shut off power or remove any of the instrument covers to perform this maintenance.

2. If paper is installed in the printer, cut the paper between the printer mechanism and the paper roll, then remove the paper roll.

3. Remove the paper from the printer mechanism by pulling it forward through the printer mechanism. If necessary, use the FEED key on the instrument keypad or the printer paper feed button (A) to advance the paper.

CAUTION: DO NOT PULL THE PAPER

BACK THROUGH THE PRINTER. THIS WILL DAMAGE THE PRINTER PAPER FEED MECHANISM.

4. Use a cleaning patch to wipe all grease from the printer bar (B).

5. Apply three drops of Nyogel 744 evenly across the entire printer bar.

6. Press either the FEED key on the instrument keypad or the printer paper feed button to exercise the printer paper feed mechanism 5-to-10 cycles to evenly distribute the lubricant over the printer bar.

7. Wipe the printer bar with a fresh cleaning patch.

8. Lubricate hte printer bar a second time with three evenly-spaced drops of Nyogel

744.

9. Press either the FEED key on the instrument keypad or the printer paper feed button to exercise the printer paper feed mechanism 5-to-10 cycles to evenly distribute the lubricant over the printer bar.

10. Reinstall the printer paper (see User’s Guide).

11. Feed paper through the slot in the printer cover, then close the printer cover to complete maintenance.

12. Instrument is ready for use.

The Advanced®Model 3250/4250 Service Manual

Probe & Stir/Freeze Wire Alignment

Proper alignment and adjustment of the mandrel, probe and stir/freeze wire are of utmost importance to assure accuracy, precision and proper operation of the instrument.

Note: After making any probe and/or

stir/freeze wire adjustments, the instrument calibration must be checked and adjusted as necessary.

Use the probe alignment tool and follow the Probe Alignment Tool instructions located in the Replacement Instructions section of this manual.

The Advanced®Model 3250/4250 Service Manual

4. Troubleshooting

Introduction

Problems with the performance of the 3250/4250 are usually identified by the software and displayed as error messages. The troubleshooting table on the following pages lists all error messages, indicates why the software generated the message, and lists what may be causing the problem.

Error messages may be caused by mistakes during testing, worn or defective parts, or parts set to the wrong specifications. This section includes directions for:

• checking and resetting the stir & freeze amplitude settings and the probe bin settings;

• aligning the probe and stir-freeze wire, and adjusting the head sensors;

• using the A/D test to check the block probe, sample probe, and cooling assembly.

NOTE: Before you repair or replace any

parts, turn the instrument off and then back on after a short delay. This allows the software to reinitialize, and may correct what appears to be a serious hardware problem.

CAUTION: Some troubleshooting requires

the instrument to be turned on while the cover is removed. Use extreme caution when operating the instrument without the cover. Hazardous voltages are present at the AC input and circuit components connected directly to the AC input. Refer to the system schematic for additional information.

The Advanced®Model 3250/4250 Service Manual

Freeze “Buzz” Amplitude

To check and reset the freeze amplitude setting:

1. Turn on the instrument. If there is a

Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. Press TEST. Press < or > to select “Stir/

Freeze Test”, and then press START.

3. At “[stir] [freeze]”, watch the tip of the

stir/freeze wire and press >. The freeze vibration should buzz loudly, and the vibration blur at the tip of the wire will last one second. The amplitude should measure about 13mm (1/2”).

If the freeze amplitude is not within the correct range, follow steps 4-6 below.

4. Remove the head cover and clear away

any metal chips between the coil frame and the clapper (A).

5. Check the position of the yoke (B) and clapper. The yoke must be 90º and flush against the back wall of the head. Bend the yoke if the clapper appears too close or too far away from the coil frame. The clapper does not have to be directly parallel to the back wall of the unit.

6. Adjust the freeze “buzz” amplitude, as described in the user’s guide Chapter 2: Changing Operating Settings:

The Advanced®Model 3250/4250 Service Manual

Stir Driver Noise

(Serial Suffix A - C, only)

Excessive noise in the stir amplitude and frequency can affect the quality of the stir signal and introduce excessive pre-freeze errors or cause poor freeze pulse repeatability. The presence of such noise can best be determined with the use of an Oscilloscope.

1. Connect the Oscilloscope between J9-1 and ground.

2. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

3. Press TEST. Press < or > to select “Stir/ Freeze Test”, and then press START.

4. At “[stir] [freeze]”, press <.

The stir wire driver signal should be a clean, square wave at approximately 72 Hertz and 4Vdc (see figure 1). If not, verify that two ferrite beads are present in your unit, as shown in Installation Option A or B, below.

Figure 2. Can be cleaned up with ferrite bead.

Acceptable if stir wire not affected.

If the beads are missing, purchase Advanced Instruments Part No. 91035 and install, as shown.

If the beads are present and you still have noise as shown in figures 3 or 4, replace the driver transistor PCB522.

Figure 1. Good signal.

Figure 3. Not acceptable. Replace stir wire driver

or use ferrite bead.

Figure 4. Not acceptable. Replace stir wire driver

or use ferrite bead.

The Advanced®Model 3250/4250 Service Manual

Installation Option A:

Ferrite Beads

NOTE: For installation option A, two ferrite beads are installed on the white wire exiting the head shaft

and attached to TR2 on the head transitionn board.

Installation Option B:

Ferrite Bead

NOTE: For installation option B, one ferrite bead is locat-

ed on the light blue wire running between PCB522 pin 4 and J3B. The second ferrite bead is located on the white wire exiting the head shaft and attached to TR2 on the head transition board.

The Advanced®Model 3250/4250 Service Manual

Head Sensor Adjustment

1. Turn off the power and unplug the instrument. Remove the instrument cover and the head cover. Place an empty sample tube in the sample well.

2. Manually push the head all the way down until the head stop screw (A) meets the base of the deck (B).

3. The head position sensors are located on the head sensor board. To adjust the head sensor position, use an Allen wrench and loosen the two bracket screws (C), and move the board (D) and bracket (E) either up or down until the head sensor flag (F) is in the middle of the head down sensor (G).

Note: To facilitate access to the two bracket

screws (C), loosen and move the printer bracket assembly.

Note: Your instrument is equipped with a

series of diagnostic LEDs on the main application board PCB605 to allow visual identification of drive signals and sensor activation.

• The yellow LEDs marked “Drive Up” and “Drive Dn” indicate that the control electronics have issued a command to the head motor relays.

• The red LEDs marked “Head Up” and “Head Dn” indicate that the corresponding optical sensor has been tripped.

The Advanced®Model 3250/4250 Service Manual

Head Up/Down Test

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required). Place an empty sample tube in the sample well.

2. Press TEST. Press < or > to select “Head Up/ Down Test”, and then press START.

3. Place an empty sample tube into the freezing chamber.

4. At “[START] Test [STOP]”, press START.

The head should move continually up and down.

Use this test to ensure the following components are working properly:

• The motor (A) is running.

• The gears (B) are turning properly.

• The sensors (C) are detecting the head-up

and head-down conditions.

• The sample well fluid passage (D) is not obstructed by debris, and the pump is moving the heat transfer fluid into the freezing chamber.

Note: If the sample well fluid passage is

obstructed, disassemble and clean the sample well following the instructions found earlier in this section. Also, examine the one-way check valve (E) to make sure it is not stuck open or closed.

Note: Your instrument is equipped with a

series of diagnostic LEDs on the main application board PCB605 to allow visual identification of drive signals and sensor activation.

• The yellow LEDs marked “Drive Up” and “Drive Dn” indicate that the control electronics have issued a command to the head motor relays.

• The red LEDs marked “Head Up” and “Head Dn” indicate that the corresponding optical sensor has been tripped.

The Advanced®Model 3250/4250 Service Manual

Power On Diagnostics

Place a tube with saline or distilled water in the sample well. Turn on the instrument At “Press START to Continue”, press START. The instrument performs a check of the cooling assembly and the probes. If a Block Probe Failure, Sample Probe Failure or Cooling System Error message displays, use the A/D tests to perform further testing.

A/D Tests

Note: You must set the correct sample and

block bin numbers before performing A/D tests.

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. Press TEST.

3. Press < or > to select “A/D Tests”. Place a sample tube containing either isopropyl alcohol or a high value standard into the freezing chamber, and press START.

Block probe in degrees Celsius, Sample probe in degrees Celsius, or Cooling block duty cycle (a % value).

Block probe resistance will indicate current temperature. The probe begins to cool after the target temperature is set, and the resistance increases until it reaches the target temperature.

Sample probe resistance will indicate current temperature. The probe begins to cool after the target temperature is set, and the resistance increases until it reaches the target temperature. Due to thermal mass considerations between the positions of the block and sample probes, the sample probe may be ±

0.5ºC of the block probe reading.

Duty cycle displays the percentage of time that the thermoelectrics power is on. When the thermoelectrics begin to cool, the duty cycle is usually at 100%. As the instrument stabilizes, the duty cycle decreases to around 60%.

4. The display will change to:

off Blk NNNN.NN ohm

readout (resistance or temperature)

channel setting (sample or block)

target temperature (off, +1 to -8)

5. Set the target temperature from +1°C to

-8°C by pressing < sequentially.

6. Press > sequentially to read:

Block probe resistance in ohms, Sample probe resistance in ohms,

Increased ambient operating temperatures do not affect the sample probe reading, but will increase the observed duty cycle, up to 85% at the maximum temperature of 35ºC.

Variations in ambient non-condensing relative humidity have no effect on the sample probe reading or duty cycle.

The following situations indicate a problem with either the probes or the cooling assembly:

• The resistance reading is replaced by the number 0. Scrolling from right to left indicates an open circuit.

• The target temperature cannot be reached, but probe readings that vary around ambient indicate a cooling system problem.

The Advanced®Model 3250/4250 Service Manual

To determine if a faulty probe is causing the

roblem:

1. Disconnect the probe from the main board.

2. Place a 6 Kohm fixed resistor across pins 1 and 2.

Sample Bin Setting

If you do not know the sample bin number, or if you want to verify the sample bin number:

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

3. If the resistance display changes to approximately 6,000 ohms, replace the probe.

If the resistance does not change, replace the main control board or application board.

If the resistance on both channels does not increase, or if the temperature reads approximately ambient while a target temperature is set, the situation indicates a problem with either the cooling assembly or application board.

To determine which component is faulty:

1. Remove the empty tube from the freezing chamber.

2. Set the target temperature to -8.

3. Raise the head manually and place a thermometer inside of the freezing chamber.

2. Press TEST. Press < or > to select “Probe Bin Test”, and then press START.

3. Place a sample of Probe Bin-Setting Fluid into the freezing chamber.

4. At “[START] Ready?”, press START.

5. At the end of the test, record the sample probe resistance and the bin number, and then press STOP. The display will change to “Probe Bin Test”.

To set the correct sample bin number, follow steps 6-8.

If the current sample bin number matches the number just recorded, press STOP twice to exit the menu.

6. Press SETUP. The Supervisor/Operator keyswitch must be in the Supervisor position.

4. If the temperature inside the freezing chamber drops to approximately -8ºC, the cooling assembly is working, and the application board needs to be replaced.

If the temperature remains at room temperature, replace the cooling assembly.

Note: When the A/D channel is open, there is

a thirty-second delay in response to any resistance change.

CAUTION: If the A/D test sample freezes,

allow it to thaw before attempting to remove the sample probe.

The Advanced®Model 3250/4250 Service Manual

7. At “Select Setup Item”, press < or > to select “Select Sample Bin #”, and then press START.

8. If the current sample bin number is not the same as the one recorded in step 5, enter the correct number, press ENTER twice, and then press STOP twice to exit the menu. Recalibration will be required.

Block Bin Setting

1. Turn off the power and unplug the instrument. Remove the cover and record the block probe bin number located on the block probe wire.

2. Replace the cover. Plug in the instrument and turn it on. If there is a Supervisor/ Operator keyswitch, turn it to the Supervisor position (if required).

4. If using a glass sample tube, check the etting by placing a sample (water or

s standard solution) in position on the probe. If bubbles form in the sample, or if the stir wire hits the tube, the current setting is too high and must be adjusted downward.

5. When the setting is correct, record the

new stir amplutide number for your records, and press ENTER.

3. At “Press START to Continue”, press SETUP.

4. At “Select Setup Item”, press < or > to select “Select Block Bin #”, and then press START.

5. Enter the correct number and then press ENTER twice.

6. Press STOP to exit the menu. Recalibration will be required.

Stir Amplitude

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. Press SETUP. Press < or > to select “Set Stir Amplitude”, and then press START.

6. At “Set Stir Amplitude”, press STOP to return to “Osmometer (or Cryoscope) Ready”.

Note: Stir wire movement should be stable.

If movement has erratic amplitude, driver transistor PCB520 may need replacement. Use an oscilloscope at J9-1 to verify the presence of a clean, square wave (see Stir Driver Noise procedure).

Display/Printer Test

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. Press TEST. Press < or > to select “Display/Printer Test”, and then press START twice.

3. Watch the vibration blur at the tip of the stir/freeze wire. If the amplitude is not within the range specified below, press > to increase the amplitude, or press < to decrease the amplitude. (The default is 100, but your value may differ.)

3250: The stir wire movement should be

no wider than the width of the sample probe.

4250: The stir wire movement should be

twice the width of the sample probe.

A continuous string of characters and numbers should scroll across the display, and a single string of the same characters and numbers will be sent to the printer.

If not, check your connections and try again. If you continue to have a problem and the instrument is working otherwise, the printer or display may need to be replaced (see Replacement Instructions for additional information).

3. Press STOP twice to end the test and exit the Test menu.

The Advanced®Model 3250/4250 Service Manual

Beeper Test

Barcode Test

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. Press TEST. Press < or > to select “Beeper Test”, and then press START and < to activate the continuous internal beeper.

If you do not hear the beeper and the instrument is working otherwise, the application board may need to be replaced (see Replacement Instructions for additional information).

3. Press STOP twice to end the test and exit the Test menu.

Note: This test will only function if Power On

Diagnostics has been completed.

1. Turn on the instrument.

2. Press TEST. Press < or > to select “Barcode Test”, and then press START to activate the barcode test. Using the attached scanner, begin scanning barcodes. Each scan should display on the instrument.

If the barcode does not display, check the connections. Try resetting the scanner to the factory defaults using the scanner manual or included Advanced Instruments documentation. Verify with the scanner manual that the type of barcode you are using is activated.

If you are still having problems, contact Advanced Instruments for service.

3. Press STOP twice to end the test and exit the Test menu.

Keypad Test

1. Turn on the instrument. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. Press TEST. Press < or > to select “Keypad Test”, and then press START to activate the keypad test. Each key pressed should respond on the display with the key name, and activate the corresponding LED, if present.

If any keys or LEDs do not respond, check the narness connections and repeat the test. Otherwise, the keypad or application board may need to be replaced (see Replacement Instructions for additional information).

3. Press STOP twice to end the test, and STOP again to exit the Test menu.

Note: This test will only function if Power On

Diagnostics has been completed.

Reset Serial Number

The instrument serial number appears when you power up the instrument. If you need to reset the serial number:

1. If there is a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. At “Press START to Continue”, press SETUP.

3. Cycle through the menu options by pressing < or > until you reach “Serial Number”, and then press START. The display will read “Serial#:********”.

The Advanced®Model 3250/4250 Service Manual

4. Press the following keys consecutively:

, 4, CLEAR. The displayed serial num-

ber will disappear.

5. Enter each digit of the serial number as it appears on the label on the back of the instrument (Note: the letter suffix at the

end of the serial number will not be entered). When the number is correct,

press ENTER.

The serial number will be recorded in memory and should appear the next time you power up your instrument.

The Advanced®Model 3250/4250 Service Manual

Instrument Software Updates

Software for Advanced Instruments model 3250/4250 instrument is contained in factoryinstalled integrated circuits called Flash EPROMs, “Electrically Erasable Programmable Read-Only Memory”, and is sometimes referred to as “Firmware”.

This type of memory is furnished in two PLCC (Plastic Lead Chip Carrier) devices on the 325620/425620/325621/425621 processor board at locations U3 and U4. U3 is the lower 8 bits of the 16-bit processor address bus, and U4 is the upper 8 bits.

Advanced Instruments products using this “FLASH” technology can be field-updated using a Windows PC and Advanced Instruments serial cable. For the latest instructions on performing the RS-232 port firmware upgrade, consult the documentation supplied with the upgrade package. For information on available updates, consult Advanced Instruments or your dealer.

4. Perform the download, using the instructions provided with the update software.

After download is complete, the instrument will reboot and report the new software version.

5. Turn off the instrument and return the Operator/Supervisor keyswitch to the desired position.

Method with cover removed

Note: Since this procedure requires opening

the case, this update should be performed only by an authorized technician.

1. Turn off the power and unplug the instrument.

2. Remove the instrument cover (see section 2 for instructions on removing the instrument cover).

Performing FLASH firmware update

Performing this update should not affect any of your calibrations or system settings unless the update has made changes to this area of memory. For specific information, consult the documentation included with the update.

Method without opening cover

Your instrument has a three-position Operator/Supervisor keyswitch.

1. Turn off the instrument. Select the 45º position between Operator and Supervisor.

2. Attach the Advanced Instruments serial cable between your PC and the instrument.

3. Turn on the instrument. The display should read “Flash BOOT Program”.

3. Locate the 325620/425620/325621/425621 processor board (smaller of two).

4. Locate SW1 dip switch on the processor board if it is a 3-position switch. Locate switch position 3 (lower switch) and select the CLOSED, ON, or 1 position. If it is a 4-position switch, locate switch position 1 (upper switch) and select the CLOSED, ON, or 1 position. Do not change any other switch setting.

5. Attach the Advanced Instruments serial cable between your PC and the instrument.

6. Plug in and turn on the instrument. The display should read “Flash BOOT Program”.

7. Perform the download, using the instructions provided with the update software.

The Advanced®Model 3250/4250 Service Manual

After download is complete, the instru-

NOTES:

1) ON = 1 = CLOSED OFF = 0 = OPEN

2) Seiko printers are used with Serial Suffix A - C; Citizen printers are used with Serial Suffix D and higher. Refer to Design Changes for additional information.

Option Switch Setup Table - 3-Position Switch

Switch Function

Selections

1 2 3

#1 Selects Instrument

3250 ON

4250 OFF

#2 Selects Printer

Citizen ON

Seiko OFF

#3 Selects Mode

Program ON

Normal Operation OFF

SW1

Option Switch Setup Table - 4-Position Switch

Switch Function

Selections

1 2 3 4

#1 Selects Mode (“PROG”)

Flash Boot ON

Normal Operation OFF

#2 Selects Printer (“OPT1”)

Citizen ON

Seiko OFF

#3 Selects Instrument (“OPT2”)

3250 ON

3250 OFF

#4 Not in Use (“OPT3”)

N/A ON

N/A OFF

SW1

ment will reboot and report the new software version.

ment does not function correctly, consult the rest of this service manual before contacting Advanced Instruments for service.

8. Turn off the power and unplug the instrument.

9. Restore SW1 position 3 (3-position switch) to the OPEN, or position 1 (4position switch) OFF, or 0 position.

10. Replace the instrument cover.

11. Reattach the power cord and return the instrument to use.

If any problems occur, check the connections and switch position settings, and then try the download again. If after download the instru-

Option Switch Settings

The processor board is equipped with a dip switch. Each switch position and combination of positions can be used to configure different programming options, such as the programing option listed above for Flash update mode.

If your settings should accidentally get changed, or your replacement processor board is shipped from the factory with the incorrect settings for your product, the following tables detail the appropriate switch positions for restoring your instrument to proper operation.

The Advanced®Model 3250/4250 Service Manual

Troubleshooting Table

Symptom Explanation Suggested Action

1. Abrupt loss of power (no display)

2. “A/D Init Failure” “A/D High Filter Error” “A/D Low Filter Error” “A/D Cal Mode Error”

Interrupted power connection or loss of power.

One or both A/D converters lost initialization.

1. Make sure the power cord is firmly plugged into both the wall outlet and the socket on the back of the instrument.

2. Temporarily unplug the power cord from its wall outlet and replace it with a known workable lamp or small appliance. Have the power restored.

3. Measure the AC voltage supplied at the power outlet used for this instrument.

4. Unplug the power cord from the power cord socket. Visually check the fuses for a blown fuse. If there is any doubt, test the fuses with a continuity checker or ohmmeter or simply replace them.

5. Check the values marked on the fuses.

6. Replace blown or incorrect-value fuses with time delay (T) fuses. Refer to instrument serial number label for proper fuse value.

7. If a fuse was blown and a replacement fuse blows too, contact our product service department for assistance.

1. Turn power off and on.

2. If problem persists, replace application board.

3. “Baudrate Error”

4. “Block Probe Failure”

5. "Block Probe Open?"

6. Buzz Point Default Set

7. “Calibration Cancelled”

8. “Calibration Out of Range; Repeat”

The baudrate setting is out of range.

The block probe has failed (usually shorted).

The block probe resistance is greater than A/D converter can measure.

The freezing point setting was outside the allowed range.

The default setting has been automatically set.

The STOP button was pressed during calibration.

The range of the samples tested did not repeat well enough for calibration.

Reset baudrate in SETUP menu.

Check the block probe resistance (2,300 ohms ±300 at room temp.)

1. Is the probe plugged in?

2. Check the block probe resistance (2,300 ohms ±300 at room temp.)

1. For the 3250, use SETUP menu to change, if desired.

2. For the 4250, this is a fixed value.

Calibration cancelled.

1. Recalibrate the instrument.

2. Check freeze pulse.

3. Check sample size and quality.

4. Check probe alignment.

The Advanced®Model 3250/4250 Service Manual

Symptom Explanation Suggested Action

9. "Check Calibration" (3250 only)

10. “Cooling System Error”

11. “Count Error; Parameter RAM”

12. Display blank or has blinking cursor

13. Display flashing or scrolling rapidly

14. EPROM Failure

Buzz point setting has been changed.

The cooling assembly may not be functioning properly.

A memory check error occurred in the RAM.

Display has experienced a voltage drop below its reset threshold.

Display is functioning normally, but is displaying an abnormal message (message length exceeds 20 characters).

This indicates a memory error with the FLASH memory.

1. Spot-check calibration.

2. Recalibrate, if needed.

1. Check the cooling assembly.

2. Make sure there is heat transfer fluid in the cooling well.

3. Verify control signal using LED D16.

4. Let unit warm up and try Power On Diagnostics again.

1. Turn the power off and on.

2. If problem persists, replace processor board.

1. Turn power off and on.

2. If problem persists, replace application board.

1. Press STOP if in diagnostics.

2. If problem persists, contact Advanced Instruments for Hot-Line Service.

1. Try restarting the instrument.

2. Replace the processor board.

15. "Error Reading Barcode”

16. ESC TRAP

17. “Event Record Lost”

18. “Fan System Failure”

The data received from the barcode reader was not formatted correctly.

Was the barcode over 13 characters long?

Indicates general operating system code fault.

The instrument has lost its record from memory.

The fan is not functioning properly.

1. Turn the power off and on.

2. Check barcode scanner settings.

3. If problem persists, replace application board.

1. Turn the power off and on.

2. Replace the processor board.

1. Turn the power off and on.

2. If problem persists, replace processor board.

1. Check fan driver/control circuit.

a. Make sure the fan is plugged in. b. Make sure the fan isn’t blocked. c. Check the fan driver LED on the appli-

cation board.

d. To check fan, disconnect and connect

to bench-top 12 VDC power supply.

2. Replace the fan or application board.

The Advanced®Model 3250/4250 Service Manual

Symptom Explanation Suggested Action

19. “Flash Boot Program”

20. ‘FPE_INTOFLOW” “FPE_INTDIV0” “FPE_INVALID” “FPE_ZERODIVIDE” “FPE_OVERFLOW” “FPE_UNDERFLOW” “FPE_INEXACT” “FPE_STACKFAULT” “FPE_EXPLICITGEN” “FPE_UNDEFINED”

21. “Head Not Up” “Head Not Down”

The instrument is configured to receive Flash EPROM updated code.

Indicates various floating point math errors have occurred.

The head up/down mechanism may not be functioning properly.

1. If intentional, perform download process as described in this manual.

2. If not intentional, turn off power and set three-position Operator/Supervisor keyswitch to Operator or Supervisor position.

3. Check processor board option switch SW1 position 3 and set to OPEN/OFF/0 (see Option Switch Setup Table).

1. Turn the power off and on.

2. Replace the processor board.

1. Check the motor, clutch assembly and relays by running the head up/down test.

2. Check the Head Up or Head Down position sensor.

3. Check LED indicators on application board.

22. ID None

23. “Insufficient Data”

24. Keypad keys unresponsive

25. Low Battery

No sample ID was recorded when test was run.

Less than two valid results were found in the requested statistics calculation.

Connections to the keypad may have become disconnected, or the keypad has failed.

The lithium battery contained inside the NVRAM memory is too low to maintain stored information, calibration, and system settings. The instrument will function normally as long as the main power remains on.

1. Are you using ID? If no, ignore message.

2. Verify ID # prints at the end of your test.

3. Reboot and check for Low Battery message.

1. Change the number of samples requested for statistical calculations.

2. Continue testing to add more results to the buffer.

1. Check circuit connections to the keypad (refer to system schematic).

2. Replace the keypad.

Contact Advanced Instruments for Hot-Line Service.

The Advanced®Model 3250/4250 Service Manual

Symptom Explanation Suggested Action

26. Low Fluid Level in Freezing Chamber

27. Memory Allocation Error

28. “New Software Version”

29. “No Parameters in RAM” “Parameter RAM Failed”

1. The heat transfer fluid pump may be malfunctioning, or there may be a leak or obstruction in the fluid system.

2. An insufficient amount of fluid is being pumped with each head up/down cycle.

Internal memory management error, allocated reserved memory conflict.

EPROM has been changed, or new software has been downloaded.

The battery-backed RAM has lost its data or has been cleared.

The instrument has lost the calibration, data, and probe bin numbers from memory.

This may occur during some software updates.

1. Evaluate the functioning of the heat transfer fluid pump per the instructions found in the Maintenance section.

2. If an obstruction is suspected, refer to Osmometer/Cryoscope Well Cleaning, located in the Maintenance section.

1. Turn power off, then on.

2. If problem persists, replace the processor board.

1. Check system settings and proceed.

2. If problem persists, replace processor board.

1. Reset sample and block probe bin numbers. Recalibrate the instrument.

2. Reset the stir amplitude.

3. Reset the buzz point (3250 only).

4. Reset plateau mode/range.

5. Reset instrument serial number.

6. Reset any other custom settings

7. If problem persists, replace processor board.

30. “No Plateau, Repeat Test”

31. No Results to Recall

32. “Out of Range”

33. Printer advances paper and appears to be printing, but the paper is blank.

The sample did not reach a valid plateau.

Recall memory was empty.

The sample tested was beyond the range of the instrument.

Paper has been installed backwards.

1. Make sure the sample is not too viscous.

2. Check the sample probe.

3. Make sure there is heat transfer fluid in the cooling well.

4. Check buzz point setting.

1. Run a test and try again.

2. If problem persists, reboot and check for Low Battery message.

3. Contact Advanced Instruments for HotLine Service.

Dilute the sample and retest.

Check to make sure the paper roll is properly installed. The paper must feed from the bottom of the roll. Refer to the User’s Guide for additional information.

The Advanced®Model 3250/4250 Service Manual

Symptom Explanation Suggested Action

34. Printer fails to print.

35. “RAM Failure”

36. “Recalibration Needed”

37. “Reset Probe Configuration”

Printer may be out of paper.

Paper feed mechanism may be jammed. Printer may have failed.

Serial Suffix D and higher: Printer cover may not be fully closed.

The main board has failed.

The calibration data has been lost frommemory. This is caused by:

a. battery back-up failure b. installation of a new processor board c. corruption of system memory d. new probe bin numbers

The probe bin data has been lost from memory. This is caused by:

a. battery back-up failure, b. installation of a new processor

board, c. corruption of system memory, or d. new software/firmware.

Check paper. If out, install new paper.

Press FEED key while oberving printer paper feed mechanism. If either slow or no paper feed is occurring, replace printer.

Serial Suffix D and higher: Make sure that printer cover is fully closed. On some models, check diagnostic LEDs D19 and D20 on PCB605.

Replace the processor board.

1. Recalibrate the instrument.

2. If error message persists after successful calibration, replace the processor board.

1. Reset sample and block probe bin numbers.

2. If problem persists, replace processor board.

38. Results not repeatable (too scattered).

39. “Result Over Range, Try Higher Setting” (3250 only)

40. “Result Under Range, Try Lower Setting” (3250 only)

41. “Sample Did Not Freeze, Repeat Test”

1. Sample probe and stir wire are not properly positioned.

2. Stir amplitude is too low or too high.

3. Freeze amplitude is too low or too high.

4. Sample probe is damaged or defective.

The sample tested in the low range setting was over the low range limit.

The sample tested in the high range setting was under 1400 mOsm.

The sample did not freeze (after “buzzing”).

1. Check sample probe and stir wire alignment and position.

2. Stir amplitude should be mixing sample briskly, but without agitation or bubbles. Check and adjust as needed.

3. Freeze amplitude should be just sufficient to freeze sample. Too low and the sample won’t freeze, too high and sample will be thrown up on the side walls of the sample tube. Check and adjust as needed.

4. Replace sample probe.

Retest the sample in the high range setting.

Retest the sample in the low range setting.

1. Make sure the sample tube is not empty, and make sure the sample is not too viscous.

2. Check the freeze amplitude.

3. Make sure the instrument is in the proper range. (3250, only)

The Advanced®Model 3250/4250 Service Manual

Symptom Explanation Suggested Action

42. “Sample Freeze Error, Repeat Test”

43. “Sample Pre-Freeze, Repeat Test”

44. “Sample Probe Failure”

45. “Sample Probe Open?”

46. “Standards Reversed? Repeat Calib”

47. “Stir System Failure”

The sample did not freeze properly.

The sample has frozen prematurely (before the “buzz”).

The sample probe has failed (usually shorted).

The sample probe resistance is greater than 10558 ohms.

The instrument was expecting “high” calibrator standard and detected a value below, equal or close to the “low” calibrator standard.

The stir/freeze mechanism may not be functioning properly.

1. The sample may have been contaminated. Retest with a fresh sample.

2. Check the freeze amplitude.

3. Check the sample probe.

1. The sample may have been contaminated. Retest with a fresh sample.

2. Check sample/block probe bin settings.

3. Check the stir amplitude.

4. Check for stir driver noise.

Check the sample probe resistance (2,300 ohms ±300 at room temp).

1. Is the probe plugged in?

2. Check the sample probe resistance (2,300 ohms ±300 at room temp).

Recalibrate the instrument with the calibrator

standards in the correct order.

1. Check the stir and freeze amplitudes.

2. Check stir driver/control circuits.

3. If problem persists, replace the application board or stir driver.

48. “Supervisor Key Needed, Press Stop”

49. “System Error: Trap” “System Error: NMI”

“System Error: Unknown” “System Error: Requests” “System Error: Primitive” “System Error: Interrupt” “System Error:

Communications”

“Illegal Error Code: “Unknown Error” “Memory Allocation

Error”

50. “Test Cancelled”

Operator/Supervisor keyswitch is in the Operator position.

The software did not execute properly.

The STOP button was pressed during the test.

1. Press the STOP key.

2. Set the keyswitch to Supervisor position.

1. Turn the power off and on.

2. If problem persists, replace the processor board.

Retest with a fresh sample.

The Advanced®Model 3250/4250 Service Manual

Symptom Explanation Suggested Action

51. “Test Time-out Error”

52. “Thermoelectrics System Failure”

53. Unknown error

54. Wrong instrument model displayed

The test has taken too long to complete.

The thermoelectric driver may not be functioning properly.

Undefined software event has occurred.

Processor board option switch SW1.

1. Make sure the sample tube is not empty, and make sure the sample is not too viscous.

2. Make sure there is heat transfer fluid in the cooling well.

3. Check the stir amplitude.

4. Check the sample/probe block bin setting.

5. Check the cooling assembly well.

6. Use A/D test to verify cooling system function.

1. Use the A/D test to check the cooling assembly.

2. Check Q1B driver.

3. Check application board.

1. Turn the power off and on.

2. If problem persists, replace the processor board.

Reset switch SW1 (see Option Switch Setup Table).

The Advanced®Model 3250/4250 Service Manual

5. Replacement Instructions

Block Probe Replacement Kit: Models 3250/4250

4D3340

Reference: Use this instruction with replace-

ment part 4D3340.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver.

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper connections may cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover, and remove the cover.

6. Remove the cable clamp screw and the cable clamp that secures the block probe cable to the heatsink.

7. Notice the length of probe cable as you

remove it from the block. Cut any tie wraps used to create the service loop, and then remove the block probe cable from the Cooling Assembly.

8. Clean out the block probe hole (B) using

a .082-inch drill (2.0 mm drill).

9. Insert the tip of the thermal grease appli-

cator all the way into the block probe hole (B). Inject grease into the hole while slowly withdrawing the tip, so that there are no air pockets. Fill the hole with grease. (The thermal grease should surround the probe when it is inserted.)

3. Remove the screws securing the instrument cover, and remove the cover.

4. Remove the six screws from the deck (A), and remove the deck.

5. Disconnect the block probe from the main circuit board.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

10. Insert the new block probe cable into the block probe hole until it will not go any further. Insert slowly so the probe is not

325P340 Rev4

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damaged, allowing the thermal grease to

e displaced by the probe. Once fully

b inserted, hold the probe cable in place until excess grease has stopped oozing out and the block probe remains in place. Wipe away any excess grease.

16. Set the new block bin number (F) (see

he following procedure).

Set Block Bin Number

11. Note the block bin number located on the block probe cable (see step 15).

12. Place the cable tie (C) on the stand-off so the tie lock faces up. Insert the washers and the screw into the stand-off (D). Tighten securely.

13. Wrap the cable tie down and around the

block probe cable and up through the tie lock (E). Pull the cable tie to tighten. Cut the excess cable tie above the tie lock.

14. Make sure the block probe cable is secure

at the stand-off, and make sure it is fastened in the block probe hole.

15. Reassemble by reversing steps 1-5.

1. If there is a Supervisor/ Operator keyswitch, turn it to the Supervisor position (if required).

2. At “Press START to Continue”, press SETUP.

3. At “Select Setup Item”, press < or > to select “Select Block Bin #”, and then press START.

4. Enter the correct number and then press ENTER. Press ENTER a second time to confirm change.

5. Press STOP to exit the menu.

6. Check calibration and recalibrate, if nec-

essary.

325P340 Rev4

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Reference: Use this instruction with replace-

ment part 3C2241R.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, 5/64” Allen wrench, probe alignment tool and instructions.

CAUTION: Improper connections may

cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the two screws from the head cover (A) and lift off the head cover.

3. Remove the two stir/freeze wire setscrews (B) from the clapper.

4. Gently pull the stir/freeze wire (C) down and out of the clapper.

5. Remove the two screws (D) that attach the clapper to the yoke.

6. Replace the old clapper with the new one and reassemble by reversing steps 2-5.

7. Use the probe alignment tool and instructions to properly position the probe and stir/freeze wire.

Clapper Replacement

3C2241R

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

3C2P241R Rev5

(4D35/3255 Service Manual)

Page 1 of 1

4250 / 4D3 Cryoscope

3250 / 3D3 Osmometer

Control Board Set Replacement

325605R/425605R

Application Board (Only) Replacement

PCB605R

Processor Board (Only) Replacement

325621R/425621R

Reference: Use this instruction with replace-

ment part 325605R, PCB605R, 325621R, 425605R, 425621R.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, static grounding (earthing) wrist strap (included).

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTIONS:

• Power cord must be disconnected to prevent electric shock.

• Never unpack, touch or handle any integrated circuit without wearing a grounding (earthing) strap to minimize your static charge.

• Instrument calibration and all customized settings will be lost when the main control board set or processor board is replaced. Therefore, make sure the current sample and block probe bin numbers and the sample stir amplitude are recorded in the service log so that you can reset these vital numbers into the replacement board.

• Refer to Option Switch Configuration section for proper model selection.

The following instructions have been prepared to cover several different replacement scenarios. Please read carefully and select the appropriate set of instructions for your situation.

GENERAL INSTRUCTIONS:

1. Record current sample and block probe bin numbers, sample stir amplitude, and any other customized settings that may need to be reset after this procedure.

2. Turn off the power and unplug the instrument.

3. Remove the screws securing the instrument cover, and remove the cover.

4. Attach the static grounding strap to your wrist and stick the adhesive end to a suitable bare-metal ground, such as the rear panel.

Board Set Replacement:

1. Remove the wiring connectors from the application board (larger of two).

2. Gently pry the board set off the four snap-on standoffs.

Note: Some instruments may require two

screws be removed prior to removing the board set.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

325P620 Rev7

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3. Remove the replacement board set from ts anti-static bag, taking care not to dis-

i turb the options/configuration switch SW1 (A), located on the processor board (smaller of two).

4. Press the new board set onto the four

snap-on standoffs so that the words “Advanced Instruments, Inc.” are located in the upper left corner. Reinstall two screws, if applicable.

Note: Some instruments may require two

screws be removed prior to removing the board set.

3. Remove the processor board (smaller of two) by first releasing the two plastic standoffs at locations E3 and E4 (B), taking care not to disturb the options/configuration switch SW1 (A). Next, gently pry the processor board off its J1 and J2 connectors (C) by rocking it from top to bottom.

5. Reconnect all wiring connections and proceed to the REASSEMBLE AND CHECKOUT instruction, below.

Application Board (larger of two) Replacement:

1. Remove the wiring connectors from the application board (larger of two).

2. Gently pry the board set off the four snap-on standoffs.

4. Remove the replacement application board from its anti-static bag.

5. Install the processor board onto the new application board by reversing step 3, being careful to firmly seat the J1 and J2 connections and aligning pins to sockets.

6. Press the new board set onto the four snap-on standoffs so that the words “Advanced Instruments, Inc.” are located

Two-Board Set

325P620 Rev7

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in the upper left corner. Reinstall two

crews, if applicable.

7. Reconnect all wiring connections and proceed to the REASSEMBLE AND CHECKOUT instruction, below.

Processor Board (smaller of two) Replacement:

1. Remove the processor board (smaller of two) by first releasing the two plastic standoffs at locations E3 and E4 (B). Next, gently pry the processor board off its J1 and J2 connectors (C) by rocking it from top to bottom.

2. Remove the replacement processor board from its anti-static bag, taking care not to disturb the options/configuration switch SW1 (A).

3. Install the new processor board onto the new application board by reversing step 1, being careful to firmly seat the J1 and J2 connections.

4. Proceed to the REASSEMBLE AND CHECKOUT instruction, below.

REASSEMBLE AND CHECKOUT

5. Turn on the instrument and verify that the instrument boots up correctly by watching the display and/or printout.

If you replaced the board set, processor board, or an older single control board configuration, you will need to enter your configuration settings and recalibrate your instrument (next step).

If you are using the original processor board (application board replacement), you will not need to reenter any settings and may skip to step 8.

6. Use the SETUP menu to reset the sample and block probe bin numbers and the sample stir amplitude, and any other customized settings.

Note: If values are unavailable, refer to the

User’s Guide for setup procedures.

7. Reset the serial number (see the following procedure).

8. Press STOP to exit the Setup menu. The instrument will run the self-diagnostics program.

9. Recalibrate the instrument. For further information, refer to your User’s Guide.

1. Verify that all wiring connections have been restored, and that they are not offset on the mating board’s connector.

2. Remove the static ground strap and put aside until checkout is complete.

3. Replace the cover, taking care not to pinch any wiring.

4, Verify that the power switch is OFF, and

then plug in the power cord.

Reset Serial Number

1. Locate a Supervisor/Operator keyswitch, turn it to the Supervisor position (if required).

2. At “Press START to Continue”, press SETUP.

3. Cycle through the menu options by pressing < or > until you reach “Serial Number”, and then press START. The display will read “Serial#:********”.

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4. Press the following keys consecutively:

, 4, CLEAR. The displayed serial num-

ber will disappear.

5. Enter each digit of the serial number as it appears on the label on the back of the instrument (Note: the letter suffix at the

end of the serial number will not be entered). When the number is correct,

press ENTER.

The serial number will be recorded in memory and should appear the next time you power up your instrument.

Option Switch Configuration:

1. Locate options/configuration switch SW1 (A).

2. For the 4-position switch, the four switch positions are numbered 1, 2, 3, 4, starting at the bottom as seen in the photo on page 2.

3. Using the table below, set the switch positions to configure the model number and options for your use.

Note: Markings on switch body may vary

[ON = 1 = CLOSED / OFF = 0 = OPEN].

Option Switch Setup Table - 4-Position Switch

Switch Function

#1 Selects Mode (“PROG”)

#2 Selects Printer (“OPT1”)

#3 Selects Instrument (“OPT2”)

#4 Not in Use (“OPT3”)

NOTES:

1) ON = 1 = CLOSED OFF = 0 = OPEN

2) Seiko printers are used with Serial Suffix A - C; Citizen printers are used with Serial Suffix D and higher. Refer to Design Changes for additional information.

SW1

Selections

1 2 3 4

Flash Boot ON

Normal Operation OFF

Citizen ON

Seiko OFF

3250 ON

4250 OFF

N/A ON

N/A OFF

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Cooling Assembly Replacement

3D3300R/4D3300R

Reference: Use this instruction with replace-

ment part 3D3300R or 4D3300R.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver.

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper tubing connections may result in fluid leakage and cause damage to the instrument.

CAUTION The heat transfer liquid contains hazardous chemicals. Consult the material safety data sheet (MSDS) and use appropriate personal protective equipment.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover, and remove the cover.

3. Remove the screws securing the instrument cover, and remove the cover.

4. Drain the system:

a. Remove the heat transfer fluid bottle

and empty the contents.

b. Replace the empty bottle and insert

the tubes.

c. Press gently on the pump at the bot-

tom of the head. Slide until all liquid has been expelled (the unit may need to be tipped on its side to remove the liquid).

5. Remove the deck and disconnect the pump (see below):

a. Remove the six screws from the deck

(A), and remove the deck.

b. Remove the two screws that secure

the bottle bracket to the cooling assembly (B).

c. Remove the two screws that secure

the pump bracket to the cooling assembly (C).

d. Disconnect the hose from the pump

mechanism (D).

6. Lift out the old cooling assembly and replace with the new one.

7. Note the new block probe bin number (E) located on the block probe cable.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

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8. Reassemble the instrument by reversing steps 1-5, making sure that there is no fluid leakage from the tubing connections.

9. Using the Setup Menu, adjust the block probe bin number, as needed. Recalibration will be required.

Cooling Fan Replacement

4D3360

Reference: Use this instruction to replace

part 4D3360 Cooling Fan.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver.

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper connections may cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover, and remove the cover.

3. Remove the screws and slide the instrument cover up and off.

4. Drain the system:

a. Remove the heat transfer fluid bottle

and empty the contents.

b. Replace the empty bottle and insert

the tubes.

c. Press gently on the pump at the bot-

tom of the head. Slide until all liquid has been expelled (the unit may need to be tipped on its side to remove the liquid).

5. Remove the six screws from the deck (A), and remove the deck.

6. Disconnect the pump (see below):

a. Remove the two screws that secure

the bottle bracket to the cooling assembly (B).

b. Remove the two screws that secure

the pump bracket to the cooling assembly (C).

c. Disconnect the hose from the pump

mechanism (D).

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

325P360 Rev1

(3255 Service Manual)

Page 1 of 2

325P360 Rev1

(3255 Service Manual)

Page 2 of 2

7. Unplug the thermoelectric connector and the block probe from the main control board.

8. Lift out the cooling assembly.

9. Unplug the fan connection (E) and remove the two nuts and washers from the fan (F or G).

10. Replace the old fan with the new one and reassemble the instrument by reversing steps 1-9.

Display Board Replacement

74051R/702041R

Reference: Use this instruction with replace-

ment part 74051R or 702041R.

The 74051R replacement display board should be used only on Serial Suffix A - C.

The 702041R replacement display board should be used only on Serial Suffix D or higher.

Tools Needed: Phillips screwdriver, flat-bladed

screwdriver, adjustable wrench, static grounding (earthing) wrist strap (included).

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTIONS:

• Power cord must be disconnected

to prevent electric shock.

• Never unpack, touch or handle any integrated circuit without wearing a grounding (earthing) strap to minimize your static charge.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover, and remove the cover.

3. Remove the screws securing the instrument cover, and remove the cover.

4. Pull the keypad panel down.

5. Attach the static grounding strap to your wrist and stick the adhesive end to a suitable bare-metal ground, such as the rear panel.

6. Disconnect the keypad and display connectors (A) from the main harness.

7. Remove the four screws (B) that attach the display to the keypad, and remove the display.

8. Replace the old display board with the

new one and reassemble by reversing steps 1-7.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

325P051 Rev1

(3255 Service Manual)

Page 1 of 1

Driver Board Replacement

PCB523R

Reference: Use this instruction to install part

PCB523R. This part should only be used with 3250/4250 instruments with serial suffix D or higher.

Tools Needed: Phillips screwdriver, small

jeweler’s flat-bladed screwdriver, static grounding (earthing) wrist strap (included).

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTIONS:

• POWER CORD MUST BE DISCONNECTED TO PREVENT ELECTRIC SHOCK.

• NEVER UNPACK, TOUCH OR HANDLE ANY PCB WITHOUT WEARING A GROUNDING (EARTHING) STRAP TO MINIMIZE YOUR STATIC DISCHARGE.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover and remove the cover.

3. Release the printer cover, remove the screws securing the instrument cover, then slide the cover forward, then up and off to remove.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

4. Locate the PCB523 driver board on the inside rear wall of the instrument.

5. Attach the static grounding strap to your wrist and stick the adhesive end to a suitable bare-metal ground, such as the rear panel.

6. Disconnect the five connectors (A) from the installed PCB, carefully noting the location of each to facilitate reconnecting to the replacement PCB. Note that the three connectors on the right side must have the latch released to disconnect.

7. Remove the two mounting screws (B), then remove the installed driver PCB.

8. Install the replacement driver PCB, secure with the mounting screws, then carefully reconnect the harnesses to the PCB, making sure to mate to the correct connector on the PCB.

9. Prior to reinstalling the instrument and head covers, test the replacement PCB as follows:

a. Connect the power cord to the instru-

ment, leave the printer cover released, then turn on the power.

b. When the display shows PRESS

START TO CONTINUE, press the TEST key on the keypad.

c. Run the HEAD UP/DOWN TEST,

listening to the sound of the motor. If the motor function seems noisy or is resonating, then adjust the potentiometer (C) on the driver PCB. Counterclockwise turns will increase the speed of the motor and should quiet the motor operation.

PCB523PM Rev0

(3255 Service Manual)

Page 1 of 2

PCB523PM Rev0

(3255 Service Manual)

Page 2 of 2

10. Replace the instrument cover, taking care

to align the cover so that there is space on either side of the printer cover, then secure with the screws.

11. Replace the head cover, then secure with

the screws.

CAUTION: DANGEROUS VOLTAGE

PRESENT. ONLY REACH IN FAR ENOUGH TO ADJUST THE POTENTIOMETER, AND AVOID TOUCHING OR CONTACTING ANY OTHER CIRCUIT ELEMENTS.

d. No other adjustments can be made

directly to the driver board. Advanced Instruments recommends testing the remaining functions (stir wire driver and thermoelectric driver) after replacing the instrument covers.

e. Turn the power off, then unplug the

instrument.

325P11 Rev1

(3255 Service Manual)

Page 1 of 2

Fuse Replacement

70011/70022

Reference: Use this instruction with replace-

ment part 70011 (1.0-Amp) or 70022 (2.0-Amp).

Voltage/Position/Fuse:

100-130 250V 2.0-Amp time delay 200-250 250V 1.0-Amp time delay

Tools Needed: Flat-bladed screwdriver.

Warning-Hazardous Voltage

CAUTION: Improper connections may cause damage to the instrument.

Instructions:

Serial Suffix A - C

1. Turn off the power and unplug the instrument.

2. Use a flat-bladed screwdriver to pry open the fuse holder door and then the fuse holder module.

3. Dislodge the old fuses and reinstall the new fuses by placing them in the fuse holder brackets.

4. Place the fuse holder back into the instrument, with the correct voltage on the fuse holder showing in the window (A) on the fuse holder socket.

5. Close the fuse holder door.

If the AC voltage of the power outlet intended for the instrument is within the 100-130V range, the window should indicate 115V. If the power outlet AC voltage is within the 200-250V range, the window should indicate 230V.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

Serial Suffix A - C

325P11 Rev1

(3255 Service Manual)

Page 2 of 2

Serial Suffix D and Higher

1. Switch the power switch to the off position and disconnect the power cord.

2. Use a small flat-bladed screwdriver or similar tool to remove the fuse holder door. Remove the fuse holder.

3. Double-check the values marked on the fuses. The Model 3250/4250 will automatically adjust for voltages between 100VAC and 250VAC, but appropriately rated fuses (2) must be installed. Use 5 x 20 mm, 250V, time delay (Type T): 2Amp

4. Reinstall the fuse holder into the back of the instrument.

5. Reconnect the power cord and switch the power switch to the on position. The instrument should start up as normal.

Serial Suffix D and Higher

Power Switch

Fuse Holder

Head Up Relay or Head Down Relay Replacement

Reference: Use this instruction with replace-

ment part 3D3109.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver.

3D3109

Head Up Relay

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper connections may cause damage to the instrument.

Note: Serial Suffix A - C, only.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover, and remove the cover.

3. Remove the screws securing the instrument cover, and remove the cover.

4. Locate the Head Up/Head Down Relay on the inside rear wall (Fig. 1). Note the wire colors and the corresponding connectors, and then remove the connectors.

Brown

Yellow

Gray

Blue

Orange

Blue

White

Head Down Relay

Figure 1

5. Remove the screws (Fig. 1A) from the relay and remove the old relay.

6. Mount the PCB502 relay board (Fig. 2) onto the standoff studs and secure the board.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

Note: As of instrument serial suffix C, the

single relay board PCB500 has been replaced by a dual relay board PCB502.

(3255 Service Manual)

325P109 Rev2

Page 1 of 2

7. K1 is the relay for moving the head up.

Connect:

Blue to J3 Orange to J4 Brown/white to J1 Yellow to J2

8. K2 is the relay for moving the head

down. Connect:

Blue to J7 White to J8 Brown/white to J5 Gray to J6

9. Reassemble by reversing steps 1-3.

Figure 2

325P109 Rev2

(3255 Service Manual)

Page 2 of 2

Heat Transfer Fluid Pump Assembly Replacement

4D3690

Fluid Filter Replacement

4D3710R

One-way Check Valve Replacement

4D3705R or 99133R

Reference: Use this instruction with replace-

ment parts 4D3690, 4D3710R, 4D3705R, or 99133R.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver.

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper tubing connections may result in fluid leakage and may cause damage to the instrument.

General Information:

Advanced Instruments, Inc. Models 3D3, 4D3, 3250, and 4250, and Fiske Associates Models Mark 2 and Mark 3 osmometers and

cryoscopes use a closed-loop circulation system for the heat transfer fluid in the cooling system. Various design modifications to the Pump Assembly affect the part replacement instructions. Please turn to the appropriate section listed in the table of contents, based upon the part being replaced and the model instrument that is being serviced.

Instruments shipped prior to 1996 have a cooling assembly inlet tube with a much smaller diameter. A tubing adapter has been provided in the 4D3690 kit to allow easy connection to this smaller tube.

Instruments shipped prior to 2004 use the same type of one-way check valve (4D3705R) in two places. One is located next to the Fluid Filter on the pump intake in the Heat Transfer Fluid bottle. The other is located at the outlet end of the Pump Assembly attached to the inlet of the Cooling Assembly. Effective in 2004, the one-way check valve

Table of Contents

Replacement Part Model Page

Heat Transfer Fluid Pump Assembly (4D3690) 3D3, Mark 3, 4D3, Mark 2 2

Heat Transfer Fluid Pump Assembly (4D3690) 3250, 4250 4

Fluid Filter (4D3710R) All 5

Bottle One-way Check Valve (4D3705R) All 5

Cooling System Inlet One-way Check Valve All 6 (99133R)

For additional information or technical assistance, please contact Advanced Instruments Hot-Line Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

(4D35/3255/MK05 Service Manual)

4DP690 Rev3

Page 1 of 7

Cooling System Inlet Check Valve (99133R)

Cable Clamp

Main Pump

Fluid Filter

Bottle Check Valve

4D3690 Pump Assembly

attached to the Cooling Assembly inlet has been changed to a different, larger check valve (99133R) to improve instrument performance and reliability.

Figure 1 shows the current 4D3690 Pump Assembly with the various components labeled.

CAUTION: The heat transfer fluid contains hazardous chemicals. Consult the material safety data sheet (MSDS) and use appropriate personal protective equipment.

4D3690 Heat Transfer Fluid Pump Assembly Replacement

Models 3D3 and Mark 3 Osmometers Models 4D3 and Mark 2 Cryoscopes

Instruction:

1. Turn off the power and unplug the instrument.

Manual Primer Pump

Figure 1

2. Remove the screws securing the instrument cover, and remove the cover (see below):

The following instructions refer to 4D3 cryoscopes without a suffix:

a. Remove the heat transfer fluid from

the front cover.

b. Remove the two screws from the rear

panel of the back cover and lift off the back cover.

c. Remove the four screws from under-

neath the front cover, lift the front cover slightly up, and then slide it forward and off.

The following instructions refer to 4D3 cryoscopes with suffix "A" or later, and to all 3D3 osmometers:

a. Remove the two sets of screws from

the side panels and the two screws from the rear panel.

b. Slide the instrument cover up and off.

4DP690 Rev3

(4D35/3255/MK05 Service Manual)

Page 2 of 7

3. Drain the system:

a. Remove the heat transfer fluid bottle

and empty the contents.

b. Replace the empty bottle and insert

the tubes.

c. Press and release gently on the pump

(A) at the rear of the cooling assembly (see Figure 2). Continue until all liquid has been expelled.

4. If present, remove the screw holding the cable clamp (B) to the cooling system shroud (see Figure 2). Firmly gripping the cooling system inlet tube (C), remove the check valve, disconnecting the pump from the cooling assembly.

7. Replace the old assembly with the new one.

a. If the instrument is a 3D3 or Mark

3 osmometer and the Spacer Strip is not present, remove the green liner

and install the Spacer Strip as shown in Figure 3. This will ensure that the proper amount of heat transfer liquid is pumped during each cycle.

b. Proceed with the installation of the

new pump assembly by reversing steps 1-4, making sure to use the cable clamp already installed on the new pump assembly. Note the orientation of the cable clamp (see Figure

4).

5. Remove the pump (A) from the bracket (D).

6. Feed the fluid filter end of the pump assembly up and around the cooling system support brackets, and remove the pump assembly.

c. Note that just above the heat transfer

fluid bottle, a small priming pump has been added to assist with priming and emptying the pump assembly. This should be positioned as shown in Figure 6.

d. Make sure that there is no fluid leak-

age from the tubing connections.

Figure 2

Figure 3

4DP690 Rev3

(4D35/3255/MK05 Service Manual)

Page 3 of 7

4D3690 Heat Transfer Fluid Pump Assembly Replacement

Model 3250 Osmometers Model 4250 Cryoscopes

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the instrument cover, and remove the cover:

3. Drain the system:

a. Remove the heat transfer fluid bottle

and empty the contents.

b. Replace the empty bottle and insert the

tubes.

c. Press and release gently on the pump

(E) at the rear of the cooling assembly (see Figure 5). Continue until all liquid has been expelled.

Orient check valve with arrow, as shown

Figure 4

4. Remove the screw holding the cable clamp (F) to the cooling system shroud (see Figure 5). Firmly gripping the cooling system inlet tube (G), remove the check valve, disconnecting the pump from the cooling assembly.

5. Remove the pump (E) from the bracket (H).

6. Feed the fluid filter end of the pump assembly up and around the cooling system support brackets, and remove the pump assembly.

7. Replace the old assembly with the new one.

a. Proceed with the installation of the

new pump assembly by reversing steps 1-4, making sure to use the cable clamp already installed on the new pump assembly. Note the orientation of the cable clamp (see Figure 4).

Figure 5

4DP690 Rev3

(4D35/3255/MK05 Service Manual)

Page 4 of 7

b. Make sure that there is no leakage

from the tubing connections.

4D3710R Fluid Filter Replacement

Note: The instrument cover does not need to

be removed to replace this part.

Instruction:

1. Open the heat transfer fluid compartment

door.

2. Remove the tube with filter attached from

the bottle of heat transfer fluid (Figure 6). Use a paper towel or absorbent cloth to soak up excess heat transfer fluid.

3. Unscrew the old filter (I) from the pump

tubing.

4. Replace the old filter with the new one,

and then replace the tube into the heat transfer fluid bottle.

4D3705R Bottle One-way Check Valve Replacement

Note: The instrument cover does not need to

be removed to replace this part.

Instruction:

1. Open the heat transfer fluid compartment

door.

Figure 6

just above the old check valve, then pull off the old check valve and filter.

4. Immediately insert the new check valve, making sure to orient the check valve properly (Figure 7).

5. Remove the old check valve from the short piece of tube that connects it to the filter, then reattach the tube and filter to the new check valve. Replace the tube with filter into the heat transfer fluid bottle.

2. Remove the tube with filter attached from the bottle of heat transfer fluid (Figure 6). Use a paper towel or absorbent cloth to soak up excess heat transfer fluid.

3. Holding the tube in an elevated position to minimize leakage, firmly grip the tube

99133R Cooling System Inlet Oneway Check Valve Replacement

Instruction:

1. Turn off the power and unplug the instrument.

4DP690 Rev3

(4D35/3255/MK05 Service Manual)

Page 5 of 7

2. Remove the screws securing the instrument cover, and remove the cover (see below):

The following instructions refer to 4D3 cryoscopes without a suffix:

a. Remove the heat transfer fluid from

the front cover.

b. Remove the two screws from the rear

panel of the back cover and lift off the back cover.

c. Remove the four screws from under-

neath the front cover, lift the front cover slightly up, and then slide it forward and off.

The following instructions refer to 4D3 cryoscopes with suffix "A" or later, and to all 3D3 osmometers:

a. Remove the two sets of screws from

the side panels and the two screws from the rear panel.

b. Slide the instrument cover up and off.

The following instructions refer to 4250 cryoscopes and 3250 osmometers:

a. Remove the two sets of screws from

the side panels and the four screws from the rear panel.

b. Lift and tilt the cover so that the rec-

tangular opening clears the front of the head cover first, then move the instrument cover back, up and off.

Fluid Flow

Fluid Filter

Figure 7

5. Firmly grip the cooling assembly inlet tube and remove the check valve. Discard the old check valve and cable clamp.

6. Install the new check valve, making sure to orient the check valve properly (see Figure 4). Connect the new check valve to the cooling system inlet tube, then fasten the new cable clamp using the hardware saved in step 3.

3. If present, remove and save the screw holding the cable clamp (B or F) to the cooling system shroud (see Figure 2 or Figure 5). Firmly gripping the pump assembly outlet tube, remove the check

valve.

4. If needed, slip the new cable clamp onto the pump assembly outlet tube.

On instruments shipped prior to 1996, the cooling assembly inlet tube was a much smaller diameter. A tubing adapter has been provided in the 99133R kit to allow easy connection to this smaller tube.

7. With the heat transfer pump assembly inlet and outlet tubes inserted into a bottle of heat transfer fluid, gently press the pump a few times to purge any trapped

4DP690 Rev3

(4D35/3255/MK05 Service Manual)

Page 6 of 7

air and to check the proper functioning of the heat transfer pump assembly.

Make sure that there is no fluid leakage from the tubing connections.

8. Replace the instrument cover.

4DP690 Rev3

(4D35/3255/MK05 Service Manual)

Page 7 of 7

Keypad Replacement

325511R

Reference: Use this instruction with replace-

ment part 325511R.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, adjustable wrench, static grounding (earthing) wrist strap (included).

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity. A static grounding wrist strap must be worn during this replacement procedure.

CAUTION: Improper connections may cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the head cover, and remove the cover.

3. Remove the screws securing the instrument cover, and remove the cover.

4. Pull the keypad panel down.

5. Disconnect the keypad and display harness connectors (A).

6. Remove the four screws that attach the keypad to the keypad support bracket (B).

7. Remove the bracket mounting screw that attaches the ground strap from the keypad to the chassis (C).

8. Remove the partially disassembled keypad from the instrument.

9. Remove the four screws (D) that attach the display to the keypad, remove the display, then mount the display onto the replacement keypad.

10. Disconnect the keypad flex cable (E)

from the keypad interface board (F). Remove the keypad interface board, then install onto the replacement keypad. Connect the keypad flex cable from the replacement keypad to the keypad interface board.

11. Remove the spring, standoff, and washer

(G) from three corners of the keypad as

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

325P511 Rev1

(3255 Service Manual)

Page 1 of 2

325P511 Rev1

(3255 Service Manual)

Page 2 of 2

shown, then reinstall them onto the replacement keypad.

12. Remove the spring, standoff, and ground strap (H) from the keypad as shown, then reinstall onto the replacement keypad making sure to attach the keypad ground under the ground strap and standoff.

13. Install the assembled replacement keypad assembly to the keypad support bracket using the four screws removed in step 6.

14. Connect the ground strap using the bracket mounting screw removed in step

15. Connect the keypad and display harness connectors (A).

16. Return the keypad to its normal, installed position. Replace the instrument and head covers, and restore power to the instrument.

Load Resistor Replacement Instruction

(Models 4D3, 3D3, Mark 2, Mark 3, 3250, and 4250)

Reference: Use this instruction to install part

425150R.

For Use On Models:

4D3 with Serial Suffix N or later 3D3 with Serial Suffix L or later MK2 with Serial Suffix G or later MK3 with Serial Suffix E or later 3250 -- All 4250 -- All

Tools Needed: Small Phillips screwdriver.

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

Instruction:

1. Turn off the instrument power and discon-

nect the power cord.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

2. Remove the instrument cover.

3. Pull and drop the keypad and display forward.

4. Looking down from above onto the chassis, locate the load resistor as shown in the photograph. In older models, it is located behind the keypad assembly and to the right of the cooling assembly. In some older models, the load resistor will not be present, but the mounting holes will still be present. In 3250/4250 Serial Suffix D or later, it will be located towards the rear of the instrument, behind the cooling assembly.

a. With no load resistor present

: Install the 425150 load resistor assembly using #2 x 3/8 screws (A) provided, sa shown in the photo. Disconnect hte cooling assembly red and black wired connector from the main harness, then plug those connectors into the mating

42P150R Rev1

(4D35/MK05/3255 Service Manuals)

Page 1 of 2

42P150R Rev1

(4D35/MK05/3255 Service Manuals)

Page 2 of 2

connectors from the 425150 load resistor assembly.

b. With load resistor present, in either

location: Unplug the existing load resistor connectors, remove the mounting screws, then remove the existing load resistor assembly, Install the new 425150 load resistor assembly and reconnect to the mating connectors of the main harness and cooling assembly.

5. Replace the instrument cover and restore power.

6. Verify correct operation using the “A/D Test” in TEST utilities.

Mandrel Replacement

3LH500

Reference: Use this instruction with replace-

ment part 3LH500.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, 1/16” Allen wrench, probe alignment tool and instructions (included).

CAUTION: Improper connections may

cause damage to the instrument.

Instruction:

1. Turn off the power and unplug the instrument.

2. Remove the two screws from the head cover (A) and lift off the head cover.

3. Loosen the two mandrel setscrews (B) and the probe setscrew (C).

4. Hold the probe gently while pulling the mandrel down past the probe and off.

5. Carefully, push the new mandrel up past the probe and into position on the flange.

6. Seat the mandrel firmly up into the mandrel hole, with the stir/freeze wire channel perpendicular to the front edge of the chassis and no gaps between the flange and the underside of the chassis.

7. Without overtightening (overtightening can damage the mandrel), re-tighten the two mandrel setscrews to hold the mandrel in place.

8. Use the probe alignment tool and instructions to properly position the probe and stir/freeze wire.

9. When the probe is in the position required, tighten the probe setscrew just enough to firmly hold the probe, but not crack or distort the thin plastic wall of the mandrel.

10. Arrange the probe leads so that they will not touch the clapper or be pinched by the head cover, and replace the cover.

11. Plug in the power cord and return the

instrument to operation.

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

3LHP500 Rev1

(4D35/3255 Service Manual)

Page 1 of 1

Figure 1

Motor Assembly Replacement

4D3350R/325304R

Motor

Head Up/ Down PCB

Capacitor

5. Disconnect the harness connected to the bottom of the Head Up/Down PCB (B).

6. Remove the motor assembly mounting screws (C) below the deck. A short or right-angle screwdriver may help to access these screws.

7. Remove the motor assembly mounting screws (D) through the top surface of the deck, then lift the motor assembly out of the instrument and place on a flat, clean surface near the instrument.

8. Measure the space between the plastic gear on the clutch assembly to the surface of the motor mounting bracket so that it can be replaced in the same location on the replacement motor. Using the 1/16” Allen wrench, remove the two Allen setscrews (E) that secure the clutch assembly to the motor shaft, then remove

Reference: Use this instruction with replace-

ment part 4D3350R or 325304R.

Use 4D3350R with Models 3250/4250 Serial Suffix A - C, 3D3, 4D3, Mark 2, or Mark 3.

Use 325304R with Models 3250/4250 Serial Suffix D or higher.

Tools Needed: Phillips screwdriver, flat-

bladed screwdriver, 1/16” & 9/64” Allen wrenches

Warning-Hazardous Voltage

Warning-Internal components may be damaged by static electricity.

CAUTION: Improper connections may cause damage to the instrument.

4D3350R Instruction:

Refer to Figures 1 and 2.

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the instrument cover, and remove the cover.

3. Place an empty sample tube in the sample well, then push the head down.

4. Disconnect the three motor connectors (A) from the capacitor and harness, noting the color and location of each connection.

325P350 Rev1

(4D35/3255/MK05 Service Manual)

Page 1 of 4

For additional information or technical assistance, please contact Advanced Instruments Hot-Line

Service Center (U.S. 1-800-225-4034, outside North America +US 1-781-320-9000).

325P350 Rev1

(4D35/3255/MK05 Service Manual)

Page 2 of 4

14. Reconnect the three motor connectors (A), making sure to reconnect in the same configuration as noted in Step 4.

15. Reconnect the harness to the connector at the bottom of the Head Up/Down PCB (B).

16. Replace the instrument cover, then secure in place using the mounting screws.

325304R Instruction:

Refer to Figures 3, 4 and 5.

1. Turn off the power and unplug the instrument.

2. Remove the screws securing the instrument cover, and remove the cover.

3. Place an empty sample tube in the sample well, then push the head down.

4. Disconnect the motor connector (G) from the driver PCB located on the inside rear of the instrument.

Figure 3

the clutch assembly. If necessary, use a screwdriver to gently pry the clutch assembly from the shaft.

9. Remove the four screws (F) that secure the motor to the bracket, and remove the motor.

10. Assemble the replacement motor to the motor mounting bracket using the four screws (F).

11. Slide the clutch assembly onto the replacement motor shaft and tighten the two Allen setscrews, making sure to locate the clutch assembly per the measurement taken in Step 8.

12. Install the motor assembly into the instrument using the two screws (D) through the surface of the deck. Make sure the teeth of the plastic gear are properly meshed with the gear rack on the sample head shaft, and the motor mounting bracket is pressed firmly against the lower back surface of the deck casting. Tighten the two screws.

13. Install and tighten the two motor assembly mounting screws (C) below the deck.

Figure 2

Motor

Head Up/ Down PCB

Clutch Assembly

Advanced 3250, 4250 User manual

Specifications and Main Features

Frequently Asked Questions

User Manual