3M VH2O2 BI Reference Guide
3M™ Attest™ Mini Auto-reader 490M System*
Quick Reference Guide
VH2O2 BI Preparation and Use
2
Inspect
1
Remove BI from pouch and reseal.
Verify media ampoule is intact and
indicator stripes on cap are blue.
Pouch
Place BI and chemical indicator (CI) in peel
pouch indicated for use in vaporized hydrogen
peroxide (VH2O2) sterilization processes.
STEAM and VH2O2 BI Activation and Incubation
Crush
1
Squeeze BI in activator to close
cap and crush media ampoule.
Flick
2
Remove BI and flick wrist to ensure
media flows to growth chamber.
3M™ Attest™ Mini Auto-reader 490M
Positive Control BI
For both Steam and VH2O2, each day a processed BI
is incubated, activate and incubate an unprocessed
BI as a positive control. The control BI should be from
the same lot code as the processed BI.
Results After Incubation
Control BI: Positive (+) Acceptable
Processed BI: Negative (-) Acceptable Sterilization
Processed BI: Positive (+) Failed Sterilization
3
Process
Seal pouch and place in sterilizer with
white side facing up. Process load as
recommended by sterilizer manufacturer.
Inspect
3
Verify media has transferred to BI
growth chamber at the vial bottom.
4
Identify
After completion of cycle, inspect and
verify media ampoule is intact and indicator
stripes on cap have turned towards pink.
Identify the BI by writing on the label.
Incubate
4
Place activated BI in any well. Display
shows remaining minutes of incubation
or test results.
DO NOT REMOVE BI until the (+) or (-)
symbol indicates the test is complete.
NOTES:
• Activate and incubate 1295 BI within
1 hour of completion of the VH2O2
sterilization cycle.
• Gloves are recommended when
activating 1295 BI.
• Allow 1491 and 1492V BIs to cool
10 minutes prior to activation.
Remaining Incubation Minutes
Touch Button (alarm off)
Interpretation of 3M™ Sterilization Assurance Core Products
STEAM Indicator Unexposed Acceptable Exposed
™
3M
Attest™ BI
1491 an d 1492V
1491 1492V
3M™ Comply™ Indicator
Tap e 1 322
3M™ Comply™ Indicator
Tap e 1 355
STEAM Indicator Unprocessed Acceptable Processed PASS Unacceptable Processed FAIL
3M™ Attest™ Steam
Chemical Integrator 1243
3M™ Comply™ Bowie-Dick
Plus Test Pac k 00135LF
3M™ Comply™ Early Warning
Test Sheet (above)
™
Comply™ Bowie-Dick
3M
Test Sheet (below)
After processing, indicator on cap turns light brown or darker.
After processing, indicator stripes turn dark-brown/black.
After processing, indicator stripes turn dark-brown/black.
1491
After processing, indicator bar crosses into the ACCEPT region. If the color bar is in the REJECT region or on the line, it is considered a fail.
1492V
PASS
When indicator ink circle on Early Warning Test sheet is lighter than the color standard, this may indicate that sterilizer maintenance should be scheduled.
VH2O2 Indicator Unexposed Acceptable Exposed Color Range
™
3M
Attest™ BI 129 5
After processing, indicator stripes turn toward pink.
3M™ Comply™ Indicator
Tap e 1 228
After processing, indicator stripes turn toward pink.
3M™ Attest™ Tri-Metric
Chemical Indicator
1348/1348E
After processing, a color change towards pink should have entered the ACCEPT window.
FAIL
FAIL
FAIL
PASS
FAIL
with a lighter colored area, usually near the center.
FAIL
3M Company
3M Center, Building 275-4E-01
St. Paul, MN 55144 USA
© 2020 3M. All rights reserved. 3M, Attest
and Comply are marks and/or registered
marks of 3M. Unauthorized use prohibited.
70-2011-7924-2 Rev 9/20
PASS
After processing, a uniform color change to dark brown/black is a pass. Fail result is indicated by a non-uniform color development,
* Refer to 3M™ Attest™ Mini Auto-reader 490M Operator Manual and 3M™ Attest™ Biological Indicator 1295, 1491 and 1492V Instructions for Use for complete
instructions and other important information. Refer to the indications for use provided in the biological indicator Instructions for Use. The indications for use should
align with the sterilization cycle to be monitored. Refer to the Instructions for Use for complete instructions and other important information related to the use of all
other 3M products included in this Guide.
Health Care Help Line 1-800-228-3957 Learn more about 3M™ Sterilization Assurance products at go.3M.com/sterilization
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