WPI LWCC-M-10, LWCC-M-100, LWCC-M-50 Instruction Manual

Instrumenting scientific ideas

WORLD
PRECISION
INSTRUMENTS

LWCC-M-xxx

Measure low volume samples with UV/VIS
absorbance spectroscopy

Serial No._____________________

www.wpiinc.com

INSTRUCTION MANUAL

111717

LWCC-M

World Precision Instruments i

CONTENTS

ABOUT THIS MANUAL ................................................................................................................... 1

INTRODUCTION .............................................................................................................................. 1

General Warnings and Cautions ........................................................................................... 2

INSTRUMENT DESCRIPTION ........................................................................................................ 2

Parts List ...................................................................................................................................... 2

Unpacking ................................................................................................................................... 2

Hardware Description .............................................................................................................. 3

Required But Not Provided (see Accessories) .............................................................. 3

Assembly ............................................................................................................................... 3

OPERATING INSTRUCTIONS ......................................................................................................... 4

Transmission Verication ........................................................................................................ 4

Noise Determination ................................................................................................................ 4

Refractive Index ......................................................................................................................... 4

Measuring in a Continuous Flow ........................................................................................... 5

Measuring Discrete Samples with WPI’s Injection System .............................................. 5

INSTRUMENT MAINTENANCE ...................................................................................................... 6

Standard Cleaning .................................................................................................................... 6

Advanced Cleaning .................................................................................................................. 6

Storage ........................................................................................................................................ 7

ACCESSORIES................................................................................................................................... 7

TROUBLESHOOTING ..................................................................................................................... 8

Typical Contamination Eects ................................................................................................ 8

Additional Information on Contamination .......................................................................... 9

SPECIFICATIONS ............................................................................................................................ 10

Related Patents .......................................................................................................................10

INDEX ............................................................................................................................................... 11

WARRANTY .....................................................................................................................................12

Claims and Returns ................................................................................................................12

Copyright © 2017 by World Precision Instruments, LLC. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.

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LWCC-M

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ABOUT THIS MANUAL

The following symbols are used in this guide:

This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.

This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.

NOTES and TIPS contain helpful information.

Fig. 1—The LWCC-M is available with 10, 50 and 100mm pathlengths.

INTRODUCTION

The Micro Liquid Waveguide Capillary Cell (WPI #LWCC-M-xxx) is a ber optic, low
volume ow cell for UV/VIS/NIR absorbance analysis; xxx represents the pathlength in
mm. The LWCC-M is available with 10mm, 50mm and 100mm pathlengths. Based on
WPI’s established liquid core waveguide technology, the analyte solution functions as
the core of a uid-lled light waveguide. Wetted parts in the sample cell light path are
PEEK, fused silica and Teon. Optical bers are used to transport light to and from the
sample cell. The cell can be used in biochemistry for DNA, RNA and protein quantication,
colorimetric nutrient and trace metal analysis, drug discovery and dissolution testing,

process control and HPLC analysis.

Similar to optical bers, light is conned within the (liquid) core of an LWCC-M by total
internal reection at the core/wall interface. Ultra-sensitive absorbance measurements
can be performed in the ultraviolet (UV), visible (VIS) and near-infrared (NIR) to detect

low sample concentrations in a laboratory or process control environment.

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General Warnings and Cautions

NOTE: Fluid pressure uctuation, cross-contamination and the introduction of small air

bubbles can cause baseline variations and measurement inaccuracies. Use the optional
Sample Injector Kit (WPI #58006) or the LWCC Injection System (WPI #89372) to minimize
the development of small air bubbles.

NOTE: WPI’s Waveguide Cleaning Kit (WPI #501609) is recommended for cleaning the
LWCC-M between uses and sample runs.

INSTRUMENT DESCRIPTION

Parts List

After unpacking, verify that there is no visible damage to the instrument. Verify that all

items are included:
(1) LWCC-M
(1) Instruction Manual
(1) Quality Control documentation

Unpacking

Upon receipt of this instrument, make a thorough inspection of the contents and check

for possible damage. Missing cartons or obvious damage to cartons should be noted
on the delivery receipt before signing. Concealed damage should be reported at once
to the carrier and an inspection requested. Please read the section entitled “Claims and
Returns” on page 13 of this manual. Please contact WPI Customer Service if any parts
are missing at 941.371.1003 or [e-mail].

Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned

(unauthorized) by collect freight may be refused. If a return shipment is necessary, use
the original container, if possible. If the original container is not available, use a suitable

substitute that is rigid and of adequate size. Wrap the instrument in paper or plastic
surrounded with at least 100mm (four inches) of shock absorbing material. For further

details, please read the section entitled “Claims and Returns” on page 13 of this manual.