LABS and K Series Cryostorage Systems
Operation & Maintenance Instructions
CAUTION - SAFETY FIRST!
• REVIEW AND UNDERSTAND ALL SAFETY PROCEDURES IN THE “HANDLE WITH CARE”
BOOKLET BEFORE ATTEMPTING TO INSTALL, OPERATE OR PERFORM MAINTENANCE
ON THIS LN2 CONTROLLER.
• DO NOT ATTEMPT TO USE OR MAINTAIN ANY LIQUID NITROGEN FREEZER UNTIL YOU
READ AND UNDERSTAND THESE INSTRUCTIONS.
• DO NOT PERMIT UNTRAINED PERSONS TO USE OR MAINTAIN THIS UNIT.
• IF YOU DO NOT FULLY UNDERSTAND THESE INSTRUCTIONS, CONTACT YOUR
SUPPLIER FOR FURTHER INFORMATION.
• BEFORE ATTEMPTING TO OPERATE THIS CONTROLLER WITH ANY WORTHINGTON
INDUSTRIES LABS OR K SERIES UNITS, YOU MUST READ THE SEPARATE OPERATING
AND SAFETY MANUAL PROVIDED WITH THAT UNIT.
Nitrogen is an inert, colorless, odorless, and tasteless gas making up four-fifths of the
air you breathe – and can be very dangerous. Air is roughly one-fifth oxygen. Liquid
nitrogen is at a temperature of -196°C (-320°F) under normal atmospheric pressure.
Cryogenic freezers are used in LN2 service only.
Extreme Cold - Cautionary Statement
Accidental contact of liquid nitrogen or cold issuing gas with the skin or eyes may
cause a freezing injury similar to frostbite. Handle the liquid so it won’t splash or
spill. Protect your eyes and cover the skin where the possibility of contact with the
liquid, cold pipes and equipment, or cold gas exists. Safety goggles or a face shield
should be worn when operating this equipment. Insulated gloves that can be easily
removed and long sleeves are recommended for arm protection. Trousers without
cuffs should be worn outside boots or over the shoes to shed spilled liquid.
Keep Equipment Area Well Ventilated
Although nitrogen is non-toxic and non-flammable, it can cause asphyxiation in
a confined area without adequate ventilation. Any atmosphere not containing
enough oxygen for breathing can cause dizziness, unconsciousness, or even death.
Nitrogen, a colorless, odorless, and tasteless gas that cannot be detected by the
human senses, will be inhaled normally as if it were air. One (1) liter of liquid
nitrogen is equivalent to 24.6 scf of nitrogen gas. Without adequate ventilation, the
expanding nitrogen will displace the normal air resulting in death.
Liquid Nitrogen System
The liquid nitrogen supply pressure at the inlet to the freezer should be in the
range of 10 psig (0.7 bar/69 kPa) to 20 psig (1.4 bar/138 kPa) for optimum
performance. Higher operating pressures will increase transfer losses and create
excessive turbulence of the liquid in the freezer, which can generate false signals
to the liquid level controller causing the freezer to under-fill. In “liquid phase”
storage applications, excessive turbulence can cause splashing which could result
in personal injury and/or damage to the freezer. When installing piping or fill hose
assemblies, make certain a suitable safety relief valve is installed in each section
of plumbing between any two isolation points. Trapped liquefied gas will expand
greatly as it warms and may burst hoses or piping causing damage or personal
injury. A relief valve is installed in the freezer plumbing to protect the line between
the customer-supplied shut-off valve and the freezer solenoid valve. Relief valves can
be piped to the outside of the building.
WARNING:
The following safety
precautions are for
your protection. Before
installing, operating,
or maintaining this unit
read and follow all
safety precautions in this
section and in reference
publications. Failure
to observe all safety
precautions can result
in property damage,
personal injury, or
possibly death.
WARNING:
Maintain adequate
ventilation to prevent
asphyxiation hazard
(see Safety Precautions).
Caution:
When installing field
fabricated piping, make
certain a suitable safety
relief valve is installed
in each section of piping
between any two
isolation points.
WARNING:
Inlet pressure should not
exceed 22 psig
(1.5 bar/152 kPa).
Higher pressures could
result in damage to
equipment.
Electrical
• This product is not intended for a life support function.
• This product is intended to be used in hospitals and clinics.
• This product has no Radio Transmitter (Intentional Radiator) functions.
• This product is not intended for electromagnetic shielded rooms only.
WARNING:
Electrical shock can
kill. Do not attempt
any service on these
units without first
disconnecting the
electrical power cord.
CS200 Control Systems
5
General Information
• This product does not intentionally apply RF energy for its function.
• This product does not intentionally receive RF energy for its function.
The liquid level controllers used with these freezers operate from 12 VDC. Disconnect the electrical power cord
from the outlet before attempting any service.
For more detailed information concerning safety precautions and safe practices to be observed when handling
cryogenic liquids consult CGA publication P-12 “Safe Handling of Cryogenic Liquids” available from the
Compressed Gas Association: CGA website: www.cganet.com; CGA customer service +1.703.788.2700; or
email customerservice@cganet.com.
General Information
The CS200 SERIES Control System can monitor and control both the liquid nitrogen level and the vapor
temperature range in the cryostorage unit you have selected. CS200 SERIES Control Systems are designed
to work with Worthington Industries Cryostorage Systems. The features are designed to provide a safe
environment for samples while at the same time tracking all relevant information associated with the freezer.
This control provides a complete historical record of the environment in your unit and therefore, the environment
in which your samples have been stored in this system. This controller features a vacuum fluorescent display.
The addition of a liquid nitrogen supply and inventory control racks for systematic retrieval of stored product
completes the total Cryostorage System.
Worthington Industries Cryostorage Systems are designed for applications where extremely low temperature
storage of biological products is required. They are also appropriate for industrial or other applications where
liquid nitrogen temperatures and high capacity are needed.
Before beginning installation or operation of this CS200 SERIES Control System, make sure that you read and
understand this manual as well as the operating and safety instructions for the cryostorage unit you will be
using with this controller.
CS200 Control Systems
66
General Information
CS200 SERIES Control System Specifications
Specifications
Control TypeLN2 Level Control & Temperature Control
Level Measurement
Sensor Type8-Thermistor Fixed (standard)
Range8 inch range (8-Thermistor)
RedundancyMultiple discrete points
Temperature Measurement Sensor Type
Type T Thermocouple
Accuracy
Resolution
Number of channels
Temperature Display Units
Electrical
Input Voltage100-240 VAC
Input Current (max)1.75 A
Input Current (continuous).5 A
Power Consumption (max)21 W
Power Consumption (continuous)6 W
Input Frequency50/60 Hz
Output12 VD C
Control Input Voltage12 VD C
Power cordAvailable for all countries
Battery
Rating12 Volt, 18Ah
TypeAGM Sealed Non-Spillable
Short ProtectionInstalled PCB with thermal fuse
Battery CoverVinyl
Solenoid Valve
Input Voltage12 VD C
Input Current.96 amps
Communications
ProtocolCryoWire Secure™
Number of Communication Ports3
1o or 1.5% of reading
.1oC
2
o
C, F, K, R
CS200 Control Systems
7
General Information
CS200 SERIES Control System Specifications (cont’d)
User Interface
Display TypeVacuum Fluorescent Display (VFD)
Buttons11
Level, Temperature and Alarm InformationVFD
“At a Glance” statusLED status wheel
Filling1 LED
Menu Access1 LED
LanguageEnglish and German
Control Tests
Power Up Self-TestControl system check
Thermistor StatusYes
Battery voltageYes
Control voltagesYes
Temperature circuitYes
Alarms
Low Level AlarmAlways enabled
High Level AlarmAlways enabled
Sensor Error AlarmAlways enabled
High Temperature Alarm (T/C #1, T/C #2)Programmable
Low Temperature Alarm (T/C#1, T/C#2 )Programmable
Thermocouple Calibration AlarmAlways enabled
Thermocouple Open AlarmAlways enabled
Power Failure (Remote only)Always enabled
Low LN2 Supply AlarmProgrammable
Battery Mode WarningAlways enabled
Lid Open Too Long AlarmProgrammable
Valve Stuck Open AlarmProgrammable
Unauthorized Access WarningProgrammable
LN2 Use WarningProgrammable
Low Battery VoltageAlways enabled
Audible AlarmAlways enabled
Audible Alarm Re-triggerProgrammable
Visual Alarm IndicatorAlways enabled
Remote Alarm DelayProgrammable
CS200 Control Systems
8
General Information
CS200 SERIES Control System Specifications (cont’d)
Buttons
PowerTurns power on/off
Fill/DefogOpen Valve
StopClose Valve
MenuAccess Menu
MuteSilence audible
EnterSave a setting or select a menu choice
Back/ExitLeave a setting unchanged or back out of menu.
Up arrowScroll the menu system or increase a value
Down arrowScroll the menu system or decrease a value
Left arrowScroll horizontal menu
Right arrowScroll horizontal menu
Data Collection
TemperatureYes
LevelYes
AlarmsYes
Memory4 Mb
Dimensions
Display Width9.5” (241 mm)
Display Height2.0” (50.8 mm)
Display Depth1.31” (33.3 mm)
Display Weight .625 lbs (.28 kg)
Main Control Width8.875” (225.4 mm)
Main Control Height6.688” (169.9 mm)
Main Control Depth1” (25.4 mm)
Main Control Weight1.0 lbs (.45 kg)
Battery Width7.25” (184.2 mm)
Battery Height6.375” (161.9 mm)
Battery Depth3.25” (82.6 mm)
Battery Weight 12.4 lbs (5.6 kg)
NOTE: Above measurements are for LABS; CS200 controllers are integrated into all K Series models.
CS200 Control Systems
9
Delivery & Returns
Unpacking and Inspection
Inspect shipping containers for external damage. All claims for damage (apparent or concealed) or partial loss
of shipment must be made in writing within ten (10) days from receipt of goods. If damage or loss is apparent,
please notify the appropriate parties as indicated below:
Domestic LTL Shipments – The customer should notify and file the appropriate damage claims with the
carrier. All products are shipped Ex Works.
Domestic UPS Shipments – Any damage should be noted and reported to shipper upon delivery, and
Worthington Industries must also be notified. Confirm with Worthington Industries Customer Service the filing
procedures for any UPS damage claims.
International Shipments – Any damage and/or claims are to be filed with the carrier. Insurance agent(s)
and Customs should also be notified.
In all cases, Worthington Industries should be notified so we can assist if needed in filing
damage claims.
Open the shipping containers; a packing list is included with the system to simplify checking that all
components, cables, accessories, and manuals were received. Please use the packing list to check off each
item as the system is unpacked. Inspect for damage. Be sure to inventory all components supplied before
discarding any shipping materials. If there is damage to the system during transit, be sure to file proper claims
promptly. Please advise Worthington Industries of such filings. In case of parts or accessory shortages, advise
Worthington Industries immediately. Worthington Industries cannot be responsible for any missing parts unless
notified within 10 days of shipment.
Freight Damage Procedures
Any freight damage claims are your responsibility. Cryostorage Systems are delivered to your
carrier from Worthington Industries’ dock in new condition; when you receive our product
you may expect it to be in that same condition. For your own protection, take time to visually inspect
each shipment in the presence of the carrier’s agent before you accept delivery. If any damage is observed,
make an appropriate notation on the freight bill. Then, ask the driver to sign the notation before you receive the
equipment. You should decline to accept containers that show damage which might affect serviceability.
Repackaging for Shipment
If it is necessary to return any part of the system for repair or replacement, a Material Return Authorization
(MRA) number must be obtained from an authorized factory representative before returning the equipment
to our service department. Contact your distributor for return authorization. When returning equipment for
service, the following information must be provided before obtaining an MRA:
1. System model and serial number, and controller model and serial number.
2. User’s name, company, address, and phone number
3. Malfunction symptoms
If possible, the original packing material should be retained for reshipment. If not available, consult
Worthington Industries for shipping and packing instructions. It is the responsibility of the customer to assure
that the goods are adequately packaged for return to the factory. All freezers returned to Worthington Industries
must be clean and decontaminated before return.
CS200 Control Systems
10
Installation
Getting Unit into Service
Your Cryostorage System comes with complete instructions for how you should
remove the unit from the crate and put it into service. Read both this manual and
your Cryostorage System’s manual before beginning any installation. Make sure to
follow any required procedures and safety guidelines when you are connecting your
Liquid Nitrogen source.
The CS200 SERIES Control System is designed to be operated at normal room
temperatures 15° C to 27° C (60° F to 80° F) at a relative humidity level below
50%. The humidity level should be maintained such that the electronics are not
exposed to condensation.
The Worthington Industries Cryostorage freezer should be positioned such that the
all sides of the unit are easily accessible and the user can easily connect/disconnect
the power cord from the wall socket.
Proper ventilation MUST BE adequate to sustain life for those working with or
maintaining this equipment.
Electrical
The liquid level controllers used with these freezers operate at 12 VDC. The external
power supply has a 120 VAC (50/60 Hz) primary. Disconnect the electrical power
cord from the wall outlet before attempting any service.
Electromagnetic Compatibility (EMC)
Although this equipment conforms to the intent of the 2004/108/EC EMC Directive,
all medical equipment may produce electromagnetic interference or be susceptible
to electromagnetic interference. The following are guidance and manufacturer’s
declarations regarding EMC for the CS200 SERIES Control System.
WARNING:
Electrical shock can
kill. Do not attempt
any service on these
units without first
disconnecting the
electrical power
cord.
The CS200 SERIES Control System needs special precautions regarding EMC
and needs to be installed and put into service according to the EMC information
provided in the following pages.
Portable and Mobile RF communications equipment can affect the performance of
the CS200 SERIES Control System. Please use the guidelines and recommendations
specified in the EN Compliance tables found on pages 50-54.
Other equipment or systems can produce electromagnetic emissions and therefore
can interfere with the functionality of the CS200 SERIES Control System. Care
should be used when operating the CS200 SERIES Control System adjacent to or
stacked with other equipment. If adjacent or stacked use is necessary, the CS200
SERIES Control System should initially be observed to verify normal operation in the
configuration in which it will be used.
The electrical cables, external power supplies and accessories listed or referenced
in this manual have been shown to comply with the test requirements listed in the
EN Compliance tables found on pages 50-54. Care should be taken to use only
manufacturer-recommended cables, power supplies and electrical accessories with
the C200S SERIES Control System. If a third-party supplier offers cables, external
WARNING:
Maintain adequate
ventilation
to prevent
asphyxiation
hazard (see Safety
Precautions).
WARNING:
If the fill fails to
stop for any reason,
quickly close the
liquid supply
valve to prevent
overfilling until the
the problem can be
determined.
CS200 Control Systems
11
Installation
WARNING:
This equipment
is intended for
use by healthcare
professionals. As
with all electrical
medical equipment,
this equipment
may cause radio
interference or
may disrupt the
operation of nearby
equipment. It may
be necessary to
take mitigation
measures such
as re-orienting
or relocating the
CS200 SERIES
Control System unit
or shielding the
location.
power supplies and electrical accessories for use with the CS200 SERIES Control
System and they are not listed or referenced in this manual, it is the responsibility of
that third-party supplier to determine compliance with the standards and tests in the
EN Compliance tables found on pages 50–54.
The use of electrical cables and accessories other than those specified in this manual
or referenced documents may result in increased electromagnetic emissions from the
CS200 SERIES Control System or decreased electromagnetic immunity of the CS200
SERIES Control System.
Power Supply Connection
Connect the power supply to your Cryostorage System and then plug the power
supply into a surge-protected wall outlet.
Validation
Some organizations require that equipment be validated periodically. If information
is needed on the proper techniques to validate this equipment, please contact your
supplier.
CS200 Control Systems
1212
Operation
These instructions are for operators experienced with cryogenic equipment. Before operating the system,
become familiar with the safety precautions in this manual and in reference publications. Make certain all
applicable provisions set forth in the Installation Section have been followed before placing a system in
operation. Study this manual thoroughly. Know the location and function of all system components.
Initial Fill
The Cryostorage System, using the CS200 SERIES Controller, comes preset from the factory. The liquid nitrogen
supply pressure at the inlet to the freezer should be in the range of 10 psig (0.7 bar/69 kPa) to 22 psig
(1.5 bar/152 kPa) for optimum performance. Higher operating pressures will increase transfer losses and
create excessive turbulence of the liquid in the freezer which can generate false signals to the liquid level
controller causing the freezer to under fill. In “liquid phase” storage applications, excessive turbulence can
cause splashing which could result in personal injury.
Control Components
CS200 Series Control System Components
The CS200 SERIES Control System for the Worthington Industries Cryostorage freezer consists of the following
components:
• Main Control Module
• VFD (Vacuum Florescent Display) Module
• Shielded Display Cable
• Wiring Harness Assembly
• Power Supply
• Thermocouple Assembly (Optional Second Thermocouple)
• Sensor Assembly
• Cryogenic Solenoid Valve
• Remote Alarm Plug
• Lid Switch Assembly
• 12 Volt, 18aH battery
• Solenoid Valve Assembly with Freezeguard
• Strainer
The CS200 Series Control System is assembled onto the freezer at the factory and completely tested. Refer to
the Quick Start Guide for freezer set-up. Start operation of the control system by plugging the power cord into
the wall outlet. Press and hold the POWER button for 2 seconds. The CS200 Series Control System will go
through a short startup routine and then start operation. Refer to Figure 2.0 CS200 Controller Display for a
description of the controller’s navigation buttons.
CS200 Control Systems
13
Operation
CS200 Controller Display Guide
Status Wheel
VFD Graphics Display
shows information about
the freezer
Fill button
usend to open
the valve
Enter Button “selects”
a menu choice or setting
Stop Button used
to close the valve
Mutes the
Valve Indicator lights
audible alarm
if the valve is open
Figure 1.0 CS200 Controller Display
Directional Buttons
Increment/decrement values or
allow movement through the system
Power Indicator
Back Button
moves out of the
menu system or
leaves current set-
Menu Indicator
ting unchanged
Power On/Off
Enter/Exit
menu system
Operational Theory
The CS200 SERIES Control System automatically maintains the Liquid Nitrogen (LN2) level
and monitors temperature in the Cryostorage freezer. Operational conditions are monitored
and any alarm is triggered if necessary. Operations data is stored in memory on the control
board.
The CS200 CONTROL SYSTEM uses thermistors to measure the LN2 level within the vessel.
A thermistor is a thermal resistor and its resistance changes as the temperature changes.
When a thermistor is submerged in LN2, its resistance will be significantly greater than its
resistance at room temperature. The control can detect this resistance change and determine
the level of the LN2 within the freezer. The CS CONTROL SYSTEM is designed to work with
an 8-thermistor assembly. There are four thermistors that can be selected to maintain the LN2
level. These selected thermistors correspond to Low Alarm, Start Fill, Stop Fill and High Alarm.
When the LN2 level drops below the Start Fill thermistor, the control opens the solenoid valve
allowing LN2 to enter the vessel. This continues until the Stop Fill thermistor is submerged in
LN2 at which point the solenoid valve is closed, preventing the flow of additional LN2 into the
vessel. The Low Alarm, Start Fill, Stop Fill and High Alarm settings can all be changed by the
user through the menu system.
High Level
High Level A
Low Level
Low Level A
Alarm
Alarm
#8
#7
#6
#5Stop Fill
#4
#3Start Fill
#2
#1
CS200 Control Systems
14
Figure 2.0 Factory Default Settings Shown
Operation
The LN2 level is indicated on the display and is determined by the number of
thermistors submerged in LN2 according to the table below:
NOTE - Offset value for LABS94K only = 3.0 in (126 mm).
Alarm Conditions
The CS200 SERIES Control System monitors a number of conditions and provides an
alarm if a problem is detected. The alarms are listed below:
Table 2.0 Alarm Conditions
Note: Please see
description of
the offset. Level
displayed may vary
depending on the
offset setting.
AlarmProblem Detected
Low Level Alarm
High Level Alarm
Sensor Error Alarm
High Temperature AlarmThe temperature detected is warmer than the high temperature alarm setting.
Low Temperature AlarmThe temperature detected is colder than the low temperature alarm setting.
Thermocouple Calibration
Alarm
Thermocouple Open Alarm
LN2 level is too low. The low level alarm thermistor on the sensor assembly is not
submerged in LN2.
LN2 level is too high. The high level alarm thermistor on the sensor assembly is
submerged in LN2.
A problem exists with the level sensor assembly. The control detects an open sensor
circuit meaning that the sensor is unplugged or the sensor assembly has been
damaged. A Sensor Error will be represented by an “O” within the Thermistor Status
menu.
The calibration data is incorrect.
A problem exists with the temperature sensor (thermocouple). The control detects an
open circuit meaning that the sensor is unplugged or the sensor assembly has been
damaged.
Power FailureNo Power.
Low LN2 Supply Alarm
A problem may exist with the LN2 supply connected to the freezer. This alarm occurs
if the freezer does not fill within the designated amount of time as determined by the
setting on the control. This may occur for a number of reasons including an empty
supply cylinder, low pressure in the supply cylinder or a closed shut off valve.
CS200 Control Systems
15
Operation
AlarmProblem Detected
Lid Open Too Long Alarm
Valve Stuck Open AlarmThe solenoid valve is stuck open.
Unauthorized Access WarningThe lid has been opened and an incorrect identification has been entered.
LN2 Usage WarningThe consumption of LN2 has increased and should be checked.
Low Battery VoltageThe voltage on the battery is low.
The lid has been opened for a period which is longer than the designated alarm
setting.
Audible Alarm Retrigger
Remote Alarm Relay
Operating in Battery Mode Alarm
Warning
The audible alarm is retriggered if error conditions still exist when the retrigger timer
expires. The retrigger time can be adjusted by the user.
The control provides a relay to provide an external signal that an alarm condition
has occurred. The remote alarm timer can be set through the menu system. This
setting determines the amount of time an error must be active before the relay is
triggered.
The power from the power supply has been disrupted and the control system is operating on battery power.
All alarms include the following:
• The flashing status wheel flashes to signal an error condition
• An audible tone sounds
• The error detected is displayed and scrolled on the screen
• The remote alarm relay changes state to provide a dry contact output signal
Lid Switch
The Lid Switch (Figure 4.0 and 4.1) is attached to the hinge and determines whether or not the lid is open on
the freezer. This also allows the control to determine whether to activate the Auto Defog, Quick Chill or Lid
Alarm features.
CS200 Control Systems
16
Lid Switch
Figure 3.1 K Series Lid SwitchFigure 3.0 LABS Lid Switch
Operation
Solenoid Valve
These units are designed to work with 12 VDC solenoid valve (see Figures 17.0, 17.1, and 17.2 Plumbing
Assemblies on page 42).
Thermocouples
Type T thermocouples monitor the temperature in the freezer. The user may choose to use NONE, 1 or 2
thermocouples with this control at any time. (The unit comes complete with one Thermocouple)
Power Supply
A 12 VDC power supply is supplied for the CS200 SERIES Control System. This system is supplied with a
universal power supply that accepts 100/240 VAC (50/60 Hz). UL approval for the system as a whole is not
required since the control operates on low voltage. If your power source differs, or is subject to disruption or
line surges due to other equipment on line, consult your Worthington Industries representative.
Remote Alarm
If an error condition occurs after a user defined period of time, a remote alarm can be initiated. This is
accomplished by connecting a remote device to the remote alarm jack on the rear electrical panel. The 3-pin
jack on the back of the unit provides continuity between pin #2 (common) and pin #3 in the normal condition.
Continuity between pin #1 and pin #2 is provided in an error condition.
Remote Alarm Connector
Figure 4.0 Remote Alarm Plug Connection
Operating Parameters
When materials are immersed in LN2, they will assume the temperature of the liquid (-196°C/-320°F). When
material is stored in the vapor phase over the liquid, the liquid nitrogen vapor is still a very cold refrigerant,
but the freezer’s interior temperature increases as product is stored higher above the liquid. This temperature
differential is not significant for many biological storage applications, and is affected by the amount of product
stored in the freezer, the type, size and material of the inventory control system, and the liquid level in the unit.
The liquid level in the freezer is determined by the position of the of the Thermistor Assembly in the sensor tube.
These sensors are set at installation to maintain a specific liquid level. A filling cycle is initiated when the level
falls below the Start Fill sensor and is completed when the Stop Fill sensor is reached. This filling cycle repeats
when the level fall below the Start Fill sensor. Sensor Probe assignments may be changed on the controller
CS200 Control Systems
17
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