World Precision Instruments PUL-1000 Instruction Manual
Instrumenting scientific ideas
WORLD
PRECISION
INSTRUMENTS
PUL-1000
Microprocessor-controlled 4-Step
Micropipette Puller
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
082318
PUL-1000
World Precision Instruments iii
CONTENTS
QUICK START .................................................................................................................................. IV
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 1
Features....................................................................................................................................... 2
Parts List ...................................................................................................................................... 2
Unpacking ................................................................................................................................... 3
INSTRUMENT DESCRIPTION ........................................................................................................ 3
Program Sequence ................................................................................................................... 5
OPERATING INSTRUCTIONS ......................................................................................................... 5
Load the Glass in the Carriages ....................................................................................... 5
Program Run Sequence ..................................................................................................... 6
Programs ............................................................................................................................... 6
Program Parameters .......................................................................................................... 7
Glass Capillary Softening Test ................................................................................................ 8
MAINTENANCE ................................................................................................................................ 9
Replacing a Filament ................................................................................................................ 9
Choosing a Filament ............................................................................................................... 10
ACCESSORIES.................................................................................................................................10
SPECIFICATIONS ............................................................................................................................ 10
TROUBLESHOOTING ...................................................................................................................11
APPENDIX A: EXAMPLE SEQUENCES ......................................................................................12
My Sequences ..........................................................................................................................12
WARRANTY .....................................................................................................................................15
Claims and Returns ................................................................................................................15
Repairs ....................................................................................................................................... 15
Copyright © 2018 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.
iv World Precision Instruments
QUICK START
1. Push the carriages together toward the center.
2. Mount the glass capillary onto the carriage, and secure it with the glass
clamps.
3. On the LCD display, choose the correct sequence. If you do not have a
sequence yet, choose one from the program storage.
NOTE: Program sequence 00 is designed for OD 1.0mm borosilicate
capillary glass (WPI# 1B100-4), and sequence 01 is designed for OD 1.14mm
borosilicate capillary glass (WPI# 4878).
4. Close the cover.
5. Press START, and the puller will do the rest. Each pull results in two
identical micropipettes.
PUL-1000
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1 The PUL-1000 is programmable.
INTRODUCTION
PUL-1000 is a microprocessor controlled, four-stage, horizontal puller for making
glass micropipettes or microelectrodes used in intracellular recording, patch clamp,
microperfusion and microinjection. The puller was designed with tight mechanical
specications and precision electronics for complete control of the pulling process
and accurate reproducibility. It oers programmable sequences of up to four steps
with heating, force, movement and cooling time. This allows graduated cycles for a
variety of applications.
NOTE: This is not a production puller designed for continual Use. If the puller stops in
the middle of a pull, allow time for it to cool down.
2 World Precision Instruments
When pulling glass tips ner that 1 um, you should rst wash the glass capillaries in
ltered acetone (or ltered distilled water and ltered 95% alcohol) then allow the
uids to evaporate in a clean dust free environment. This assures that submicron
tips are not immediately clogged with glass particles from the manufacturing of the
capillaries.
A glass capillary is heated by a platinum/iridium lament and pulled by a controlled
force. The PUL-1000 is capable of producing a vast array of pipette shapes.
Pipettes–PUL-1000 can produce pipettes with tip diameters from less than 0.1µm to
10+ µm. Microprocessor settings control the pulling automatically.
Pulling pipettes is an art, and reliable results depend on factors like the operating
environment, the type of glass used and your technique. Understanding how the
puller works is critical to manufacturing the pipettes you want. Glass capillaries
can even be slightly dierent for every lot. Each time you open a new vial of glass
capillaries, recalibrate the puller heat parameters for the new glass.
Programming–The settings for a 4-stage pull may be stored in memory. Up to 95
user-selectable programs can be permanently be stored in memory for later recall.
The instrument contains two factory installed and tested programs. Choose from the
factory installed programs or create your own.
Construction–The cover of the pulling chamber is made with tempered glass to
minimize the eects of humidity and air currents on the reproducibility of pulled
pipettes.
Power Supply–PUL-1000 has a switching power supply for use anywhere in the
world without worry about the line voltage dierences. Pulling reproducibility is
unaected by line voltage uctuation. Heating voltage can be controlled to within 0.1%
accuracy even when line voltage uctuates from 100–240VAC.
Features
• Microprocessor controlled
• Program sequences up to four pulling steps
• Produce micropipettes with a tip diameter less than 0.1µm or greater than 10µm
• Store up to 95 programs in memory
• Two factory programs installed
• Tempered glass cover reduces the eects of humidity and air currents on puller
reproducibility
• Switchable power supply for any line voltage 100–240VAC ensures that line
voltage uctuations don’t aect reproducibility
Parts List
After unpacking, verify that there is no visible damage to the instrument. Verify that all
items are included:
(1) PUL-1000 Puller
(1) TW100-4 - Thin Wall Glass Capillaries, 4 in., 1 / 0.75 OD/ID, package of 500
(1) MF34G-5 package of MicroFil
(1) Instruction Manual
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