Wild Leitz Laborlux S User manual

I

1 Care and Maintenance

Dust protection is provided by a fle

s

hould

alwa

ys

be us

ed

when the ins

stand

should
ther cloth;
petro

leum

spots

on the object

oil or

fin
Particular care
using acids
these
under
cleaned aft

Du

st can be removed

fine-haired
brushing. If the
tened
eff

ec

t, pure alcohol may

taken

pi ece

surfaces and

coa

ting

be corre spondingly car efully clea
Objecti ves s
damage

to us

for

is also advis

Micros

special
do

es not
rough a
whose inside
roo m.

plugged with co

this type

be

cleaned
alcohol
is well

vaseline.

substances

all c

er use.

with distilled

when

s of approx

or

dirt

repair.

copes being used in

care

occur,

nd met

It

mu

of storage is

must

suited

stage can

should

or

other

aggressive c hemica ls.

with

ircumst

.

anc es,

The

optics

from

brush,

blowing

din

is difficult to remove, a cle

water

clea

ning

anti~ref1ection

the

imat

ely the same hardn

hould

not

is noticed ins

Cleaning

ed

against.

It

should

which

iculous cleaning

temperature

st also

tton

be

wool

is

not

from

time to

nol

be

us ed as it

for clea

the

front

be

be

ning

be

rem

oved

be

taken

when

stand

or op tics

and

all

must

be

glass s

urf

gently

, c

an

be used or,

be

applied

elements

ned

screw

ed

ide them, th

of

the

inn

er surfaces of the eyepiec

hot

and

lor

ensured

managed, in the fir st place, by tho-

and

is at

least 5OC

provided with airing holes, loosely

or gauz

e as

possible,

xib

le dust cover

trument

th e paint

parts should

across the surfa

. Particular

coatings. The

apart

that a build-up

protection

the

is not in u

tim

e with a

altacks the paint,

by rubbing with paraf-

undertaking studie

kept scrupul

aces

of the objectives have

ess

.

ey

humid

storage in a cupboard

microscope must

linen

ed

surfaces.

Dire

ct

contact

must

be avoided

be ca refull y

ously clean.

by mea

during

should

ns

of a dry>

ce

an cloth

if

trus also h

care should

outer

as glass and must

cleaning.

be return

cJimat

es req
of

above

that of the

aga inst dust.

which

se. The

or

lea-

but

Pale

of

whil

st

, mois-

as

no

be

eye-

If

ed

es

uir

fungus

If

be

kept in a
age

nt (e.g. silica gel).
laboratories
du

st is

the

be

cove

red with
ing and s
one month

s

above, is desirable.

Proper
vice. If, however, a

please

direct.

handling

contact

Technical Service

Ernst

Leitz

postr.ch

D-6330
West
Telex:

e

Wetzl.r

Germ.ny.

483727

clos

ed container

with

greatest

tor

ed

in

occurs,

Wetzl.r

2007

,

eltsc

with

an

These measures should

air conditioning. In

enemy. The ins

the dust cover

a c

upboard. Ifa

storage

of

the mi

check

your Leitz

GmbH,

immediat

in a warm cu

croscope

over

agency

adequate

trument

humid

will

or

repair becomes necessary,

or

warm

should,

e ly

period

pboar

ensur

our Tec

amount

and

after

e decades ofse

be ta

of

d,

hnic

of drying

ken

dry

climates)

ther

use

or clean-

lon g

as

described

al

Service

even

er

in

efore,

than

r-

2

Fig. 1

Leitz

LABORLUX

condenser no.

1

Eyepiece

S with stage no.

56

and binocular tube

11,

S.

2 Binocular lube S 45°

3

Analyser

4

Objectives

5 Specimen stage no.

6 Coaxial drive for

7 Screws
8 Height adjustment
9 Condenser

10

Adjustable

11

Centring screws for condenser mou

12

Condenser no.

13

Field di

14

Lamphousing

15

Mains

slit

11

to

moving

attach object gu

of

mount

height

the condense r

with slide changer

stop

of

56

aphragm

20

switch (concealed)

th e

specimen

id

e n

o.

12

the

condenser mount

nt

moun

t

3

2 Assembling the microscope

Mounting the lubes

Press the lever in the direction

can

DOW

be inserted

tube

has been inserted allow the lever to slide back.
can be rotated by
pulling the leve

Fi

g.

2 Mounting the tub

into the quick-change mount. Once

0

360

and clamped in any position by slightly

r.

es

of

the arrow (Fig. 2); the tube

The

the

tube

Inserting the eyepieces

The

eyepieces are insert

ed

into the eyepi

ece

tub es (Fig.

3).

For
the LABOR LUX S, Leitz eyepieces are used whic h are calcu­lated for the mechanical
pieces are distinguish
the additional ident
magnification, e.g.

tiflcation

(order no. 519653)
the side

Fi

g. 3 Inserting t

oft

he field

of

the tube.

must

he

eyepieces

tube

length

of

160

mm. Thes

ed

from those for 170 rum tube length

ification ofthe fie

JOx/20.

If

ofview

Leitz eyepieces without this iden-

ind ex are to be

ld ofview index after the

used

be pushed over the eyepiece

e eye-

by

, a spacer ring

mount

on

4

The total magnifica tion

rep

roducti

on

ratio

of

(x

tube factor).

Example:
25/0

.5

0 objective

IOx/18

eyepiece
tube fact or Ix
To

tal magnificatio n:

25 x 10

of

the microscope is given

the ob

jective x eyepiece magnification

x 1 =250x

by:

Scre

wing

in

the objectives

Screw the objectives into the nosepi

ti

on

that stepwise magnifica

4, 10, 40, etc).

All Leitz microscope objectives from a magnification

chan

ece

(Fig.

4)

in

such a way

ge

is

possible (e.g. in the order

of

2.5x

and calculated for a tube length of160 mm can be used on th e

AB

Lei tz L

mjnation capacity

ORLUX

S.

Please no te

of

the

in

condensers.

th

is

cootext the field

iUu

Microscope objectives calcul ated for 170 mm tube length can

6x

be used from 1

magnifica tion.

-

Fig. 4 Screw

ing

in the objectives

5

Identification markings on the objecti'ves

(Fig.

5)

1 Mechanical tube l

The distance

the

tube

.

engt

h

in

mm

from the objective sho

ulder

to the edge

2 Cover glass

The

A

dash

engraving

(-) instead ofa

0.17

is the thickness of the coverglass.

number

indicates that

the

objective

be used for specimens with or without a coverglass.

Field

3

Oatness

EF objectives a

of

up

to

18

PLAN

objectives are system s with a

to 22.5

mm

Fig. 5 Objectiv e identification

of

the objective

re

systems with a more

or

less flat

mm intermediate image diameter.

flattened field

intermediate

image diame

ter.

fi e ld ofview

of

can

of view up

If

there

is no indica

achromat with optimum correction,
of view

inde

x.

tion

of

field flat

tening,

for

use with

the objecti

4 Reproduction scale in the intermediate image

im

Size ratio between the intermediate

10

: 1

age and the object, e.g.

5 Numerical aperture
Physical identific ation

of

the objective's resolving power

6 Immersion medium

Immersion media can be, for example.

ine

(GLYC). The objective

must

il

. water (W) or glycer-

o

always be used with

engraved immersion medium.

Bef

ore the immersion objective is focused, a drop

sion

medium

bub

bles .

Ph

ase cootrast objectives

7
Objectives with

The

se objectives have

achromats

side

annular
PHACO
contrast
to be found

should

ph

ase ring

be

green

with black sleeves).

stops necessary for

3) in a

slide

or

condenser

on the

(e.g.

phase contra

ap plied to

for

phase contrast observation.

writing (w

The

label for the

the

obje

ith

these objective

in

the

turret

platc

of

PHACO

3= set

turret

st objectives.

ct

the

tbe

plate

ve

is

up

to

18

field

of immer-

without

exception

condenser-

s (e.g.

UKL

phase

to 3) is also

an

the

air

of

ured

ring

8 Colo

indicating

the objective

magnification

9 Immersi on objectives

6

Allaehing Ihe objeel guide
Th

e objecl guide no.

allached to the left

12

for left- or right-hand

or

right

of

the

specimen stage with two

ope

ration is

screws (6.l).

Inserting the condensers

Rotate the

knob and on the

condenser

conde

cla mp (2) until the dots on the knurled

nser mount coincide. Then lower lhe

condenser USing the knob (7.1) until the condenser (7.6)ca n be

eas

ily pushed into the mount

condenser clamp in the direction orthe arrow. The condenser

is now firml y fixed in the slid e

UKL

are inserted in Ihe s

ame

as

far

chang

way as

as

the stop.

er. Condense

condenser

Now

no.

turn the

rs

LK

56

(ill us-

and

Ira ted, 7.6).

Fig. 6 Attaching the object gu ide

1

Fi

xing screws for objecl guide no.

-

.,.-

12

Cond enser no. 56

With the

ti

on is pns>ible for a

ficati on

and out

Cond
oil cap n

ra

ised to A

th

e conden ser and the specimen. To screw the oil cap on , the

protecti

F

ig.
1 Height adjustme
2 Condenser clamp

3 Condenser holder

4 Cen
5 Adjustable heigh t stop for the condenser holder
6 Conde nser

two·lens

at

fi eld

of

the front lens.

enser no .

o.

512652

1.2

ve

ring mu

7 Inserting the condenser

tring screws for c

co

nden~e

ll

object

of view index

56

works with th e nume rical ap erture A 0.90. If

is

screwed on, the

5 when Leitz i

st

be screwed

nt

of

the condenser holder

ondenser

no. S6

r

n

O.

56.

homogeneo

fields

orobjcc

20

without

mme

rsion oil is applied betw

ofT with

mount

t;ves from 4x magni-

additional swinging

num

erical aperture

a knurled ring.

us illumina-

can

in

be

een

7

The

aperture
scale for reproducible s
The

slit at the side is inte nded for the insertion

annular stops
tion. These

after the le
Condenser

The

LK condenser has a hinged co

con

denser

illuminate aU object fields

with field

higher the condenser

diaphragm

for

simple

can

only be

ver

has been pressed.

is

adjusted with the

etting

of

the

darkfieid
taken out

LK

top isswung out, the lower

of obj

of

view index

20. When using objectives of

top

must

be

swung

or phase contrast illumina-

of

ndenser

ecti ves from 2.5x upwards

the handle (8.2).

Fig. 7b

Cond

ens

er

no.

I. Protective ring

2. Locking lever for

3. Slil ror slide with

4.

Lever for adjustment of aperture diaphragm

5. Dovetail guide

56

f>

hde with

diff

difTusion disc and annul

usion disc and annular stops

lever There

condenser

condenser n

top S

part

of the

in

. This is done with

diaphragm.

of

slides with

o.

56

again

1.1.

When

cond eoser

lOx

ar

stops

is

a

With condenser
works with
field

diaphragm

gla

ss medium

The condenser top

a thread

ap~rlur

dens

th e

the S

can

can be found in th e tabl e on page 10.
The

and

Ther

with

e

or tops

er to

ps

1.1

standard top.

aperture iris

e

is

top A 0.90 S

the

numerical

is produced with the

1.2

mm

over the stage surfac

of

the LK condenser

other condenser tops with a higher

with a long

D 0.80 or D 1.19 can also be screwed

Furth

diaphragm

a scale for reproducible setting

diaphragm.

Fig. 8 Co

t A 0.

2

J

90

H<lndl

Lev

er

ndenser

S

I.l

Condenser top

e

to

swing

for

adju

LK

out

stment

th

e

of the ilperture

1.1

swung in,

aperture

er working distance.

the conden

A 0.90.

Th

e image of

cond

enser top S

e.

is

interchangeabl e via

Darkfi

on

er

information

on condenser tops

is adjusted with thc lever (8.3).

of

the

condenser top

diaphrn

gm

se r LK

the

1.1

in a

num

erical

eld con-

instead

condenser

of

8

Condenser

Its performance features in brightfJe
the condens er no.

UKL

56.

ld

are the

same as

those

of

The annular slOPS necessary for phase contrast or darklield illu-

mination are situated in a turret plate (9.4) for q uick chaoge.
P

os

iti

on "

H"

(9.3) of the turret is for brightfle

inves tigations. Posit ions

SlOPS

PHACO

of

the objective

(9.2). Positi

wi

th objectives from

Th

e wheel (9.5) is for ad

1,2 and

in lise

on

4 is occupied by an annular st

I,

2 and 3 accommodate the annular

3.

These can be aligned to the phase ring

by

lOx

pushin g

to 40x.

in

the two centring screws

justing the ape
necessary for brightfield investigations.
phase contrast

F ig. 9

UKL

1 Adjustment tel

2 Cen tring

3 Turret

4

Tu rret

pl
pla

5 Adjustment wheel

or

darkfield (PH position).

conden

se

r

escope

for

screws

ate position indicati

(pr

te

ess

wi

th

io

sca

ct:
to use)

ntri

ng

the annular

on

le for

aperture diaphragm

rtur

It

op

e di

aph

is fully

stop

s

ld

(Hellfeld)

for darkf.eJd

ragm , only

opened

for

Darkfield condensers

The condenser
with

lOx

to

40x

compar

ed w

ith the darkfieJd produced w

The condenser D
toge ther with oil
ser caps

screwed

Fig.

J

Co

2 Oil

3 Lower pari

4 Sp

diaphra

5 Knurled ring for adjustment

6 Funn

are

also available without

onto

the bottom

10

Darkfield objec

ndensertop D

co

ndenser

ec

of condense

ial darkfield objective

gm

el

slop for the o

00.80

D 0.

80

is

objectives.

1.1

9 has an oil

immersion

tive and

0.80 -0.95

top

D

1.

19 -1.44

r with dovetail guide

il

immersion objective

intend

and

D!.l9

ed for darkfleld investigat ions

Imag

e contrast

imm

is

optimised he

ith

annular

ersion cap and is used

objectives. The darkfield

part

funnel

of

bottom

th

e

stop

parts and can be

LK

condenser.

SI.J

EF

100/ 1.

25

-0.60

Oel wilh built-in i

oflhe

iris di

aphrag

m

stop

con

re

as

s.

den-

ri

s

9