VWR International 634-6002, UV-3100PC Instruction Manual

VWR Collection Manual ver 1, rel 15.05.2007

UV-3100PC Spectrophotometer

INSTRUCTION MANUAL

European Catalogue Number(s): 634-6002

Version: 1.0.0 Issued: 07 , April 2010

Legal Address of Manufacturer

Europe

VWR International Europe bvba Researchpark Haasrode 2020 Geldenaaksebaan 464 B 3001 Leuven Tel.:+ 32 16 385 011 http://www.be.vwr.com

Made in China

Table of Contents

Part 1: Spectrophotometer Safety Information 1 Package Contents 1 Unpacking 2 Installation 2 Intended Use 2 Symbols and Conventions 3 Specifications 3 Overview 4 Description of Buttons and Switches 4 Operational Keys 6 Getting Started 8 Important Guidelines 9 General Operating Instructions 10 Operation 11

Basic Mode 12 Quantitative Mode 14 Wavelength Scan 19 Kinetics 21 DNA/Protein Mode 24 Multi Wavelength Mode 26

System Utility 28

Troubleshooting 34 Repair and Maintenance 35 Daily Maintain 35 Spare Parts Replacement 36 Accessories and Spares Parts 41 Technical Service 43

Warranty 43 Equipment Disposal 44

Part 2: Software Functions 45

Main Functions 45 Spectrum Processing Function 46 System Check and Calibration Function 46

Installation 47 Introduction 50 Operation 55

Single Wavelength Photometric Measurement 55 Fixed Point Measurement 55 Wavelength Scanning 61 Time Scanning (Kinetic Analysis) 73 DNA/Protein Measurement 75

Appendix 1 78 Appendix 2 78

Part 1: Spectrophotometer

Safety Information

Please follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

VWR recommends against the use of UV-3100PC Spectrophotometer.

 Do not open the device.  Disconnect the device from the mains supply before carrying out

maintenance work or changing the fuses.

 The inside of the device is a high-voltage area Danger!  Do not use the device if it is damaged, especially if the main power

cable is in any way damaged or defective.

 Repairs may only be carried out by the service technicians from your

local VWR office and authorized contractual partners.

 The device must be connected to a power outlet that has a protective

ground connection.

 If the equipment is used in a manner not specified by the

manufacturer, the protection provided by the equipment may be impaired.

 Do not allow any liquid to enter into the device.



Do not operate the device in a hazardous location or potentially explosive environment.

Package Contents

Description Quantity Spectrophotometer 1PC Glass cuvette 4PCS Quartz cuvette 2PCS Power Cord ( Euro Plug ) 1PC Power Cord ( UK Plug ) 1PC

Power Cord ( SW Plug ) 1PC USB Cable 1PC CD-ROM 1PC Manual 1PC

Unpacking

Open the package, according to carefully check the packaging packing list items, if found inside the packaging are missing or damaged items please your local VWR office and authorized contractual partners.

Installation

Placement

Place the instrument on the stable table carefully.

Install printer (Optional)

Check to confirm instrument power switch is turned off, connect the printer’s data cable to

the Instrument’s parallel port.

Link the power cord

Check to confirm instrument power switch is turned off, the power cord plug into two separate power interface and power supply socket apparatus.

Intended Use

UV-3100PC Spectrophotometer used in Chemistry, Pharmaceuticals, Biochemical, metallurgy, Light Industry, Textile, Material, Environments, Medical, Education and some other fields. It is one of the most important instruments in Quality Control and an essential in normal laboratories.

Symbols and Conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION This symbol indicates a potential risk and alerts you to proceed with caution

CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution

CAUTION This symbol indicates risks associated with hot surfaces

Specifications

Optical System Single Beam Wavelength Range 190-1100nm Band Width 2nm Stray Light ≤0.05%T @ 220nm & 360nm Photometric Range 0-200%T,-0.3-3.0A, 0-9999C Wavelength Accuracy ±0.5nm Photometric Accuracy ≤±0.5%T or 0.005A@1A Stability 0.002A/h @ 500nm Memory 32K Language English, French, German, Spanish Display 320×240 Dots Matrix LCD Interface USB, Parallel Measuring Procedure Photometry, Quantitation, Wavelength Scan,

Kinetics, DNA/Protein, Multi-wavelength

Power Supply AC 110/220V, 50/60Hz Dimension 490×360×240mm Weight 14kg

This instrument is compliant to the European Directives on

Low Voltage Directive 2006/95/EC Electromagnetic compatibility 2004/108/EC Restriction on use of Hazardous Substances RoHS 2002/95/EC and theirs amandements.

Overview

UV-3100PC Spectrophotometer has the characters of wide range of wavelength, high sensitivity, powerful function, easy to use, simple structure and pretty figure. Besides these, the large LCD, High Precise A/D and easy to store RAM makes the instrument much more superior than other originals. It is widely used in Chemistry, Pharmaceuticals, Biochemical, metallurgy, Light Industry, Textile, Material, Environments, Medical, Education and some other fields. It is one of the most important instruments in Quality Control and an essential in normal laboratories.

Description of Buttons and Switches

Front View

Rear View

Operational Keys

Key Functions

Function Keys: Functions on-screen prompts

… ,

Numeric Keys: Enter numbers and letters

CLEAR Key: Delete the input value or stored data

ESC Key: Return to previous Interface

100%T/0Abs Key: Blank

OPEN Key: Open files stored in internal memory

SAVE Key: Save files to internal memory

START/STOP Key: Start/Stop testing

GOTO ʎ Keys: Set wavelength

PRINT Key: Print measuring result

ENTER Key: Confirm operation

CELL Key: Select/Deselect Auto-cell Holder

RIGHT, LEFT Keys: Search peak/valley and set X

scale

UP, DOWN Keys: Scroll menu/data and set Y

scale

Getting Started

The following chart describes the basic operation of the instrument.

Turn On and Self-check

Switch on the power. Then the instrument begins to self-check and 15 minutes’ warm up. Self-check includes the following steps:

WL: 500.0nm 10:00:00

VWR International bvba

Wait until EasyRTOS booted:

Turn on lamps →RAM Check→ Start RTOS kernel→ Initialize Comm. Port → Initialize Printer → Initialize AD → System position → Warm up.

Press ESC to skip…

WL: 500.0nm 10:00:00

Wait until EasyRTOS booted: Warm up 15 minutes…………?

After warm up, the instrument will ask the user to re-calibrate the system. Users can decide if they need to re-calibrate the system or not. After this step, the instrument can work normally.

Wait until EasyRTOS booted: Warm up 15 minutes…………?

WL: 500.0nm 10:00:00

System calibration? No

Cancel Move Dn Select

WL: 500.0nm 10:00:00

Main Menu

① Basic Mode

② Quantitative Mode ③ Wavelength Scan ④ Kinetics Mode ⑤ DNA/Protein Mode ⑥ Multi-Wavelength Mode ⑦ System Utility

Important Guidelines

 Reagents and dilution buffers can cause cauterization and other damage to health.  Samples (nucleic acids, proteins, bacteria cultures) can be infectious and cause

serious damage to health.

 During sample preparation, measuring procedures and maintenance and cleaning

work, observe all local laboratory safety precautions (e.g. wear protective clothing and gloves, use of disinfectant) regarding the handling of sample material.

 Dispose of measuring solutions and cleaning and disinfectant materials in

accordance with the relevant local laboratory regulations.

General Operating Instructions

Select Application

Main menu, press numeric keys or use the key , to choose corresponding menu,

then press to enter into.

Set Wavelength

Press to set wavelength, use numeric keys to input the values, press to confirm and go to the point you set, then do blank automatically.

Set Parameters

In different application, press function key to set parameters, press , to choose or

input the values by numeric keys, press to enter into, press to return.

Set Auto-cell Holder

Press to active the auto cell holder and press the numeric key (1-8)to make

corresponding cell position at the light path. Press again to inactive the auto cell holder.

Delete the Input Value

Press to delete a character, press to delete all the characters.

Delete the Test Results and Stored data

Press to delete the test result or stored data.

Blank

Put the Reference in the light path, press to do blank.

Measure Samples

Put the samples in the light path, press to measure.

Print the Test Results

Press to print the test results.

Store the Test Results

Press to store the test results, input the file name by numeric keys and press

to save.

Load the Stored File

In the test interface, press to go into file selecting interface, press , to

choose the file you want, press to open.

Operation

Self-check

Remove all the blocks in the light path and close the lid of the compartment; Switch on the power supply to begin the self-test.

Warm up

After self-test, the instrument goes into pre-warm state. For accurate test, at least 30 minutes of warm up is required.

Check the cuvettes

The cuvettes must be clear and there’s no remains of the samples on the surface of it. Only Silicon (Quartz) cuvettes are permitted to be used in the range of UV area.

Basic Mode

1. Enter into Basic Mode

Main menu, press numeric key or , to choose “Basic Mode”, then press

to go into.

2. Set Photometric Mode

Press to set photometric mode. Press , to choose “Abs.”, “T%” or

“Conc./Factor” and press to confirm. If users choose “Abs.” or “T%”, please go to step 5 directly.

3. Set Concentration Unit

Press to set concentration unit. Press , to choose unit followed with pressed to confirm. You can also choose “other” to input the self defined unit.

4. Set “Factor” or “Standard”

Two methods are under your choice:

Method 1：：：：Input Factor F

Press to set F. Input the value of F by numeric keypad, press to confirm. Then the F value would display on the screen.

Method 2：：：：Standards Mark

Put the reference sample in the light path and calibrate 100%T/0Abs; Put the

standard sample in the light path，press to start the mark. Input the

concentration value of the standard and press to confirm, then it displays on the screen.

5. Set Wavelength

Press to set wavelength, input the value by the numeric keypad followed with

pressed to confirm.

6. Blank

Put the Reference in the light path and press to do blank.

7. Measurement samples

Put the sample to be measured in the light path, then the result displays on the screen automatically.

8. Print the Test Results

Press to print the Test Results.

Basic Mode Test Report

Wavelength: 500.0nm Result: 0.000 Abs Date & Time: mm-dd-yyyy, hh:mm:ss

Model: UV-3100PC SN: UEFXXXXXXX Version: A1.176 VWR International bvba.

Quantitative Mode

1. Enter into Quantitative Mode

Main menu, press or press , to choose “Quantitative Mode” followed with

pressed to confirm.

2. Set Unit

Press to set concentration unit, press , to choose and press to confirm.

3. Set up Standard Curve or load the stored curves

Press to go into set up interface, 2 methods are under your choice.

Set up Standard Curve:

Method 1: Input Regression Equation

1) Set Fit Curve Method. Press to set Fit method，use , to

choose the method and press to confirm.

2) Set Wavelength. Press to set wavelength. Use , to choose

measure method, then press to confirm. Input the wavelength value

you need and press to confirm.

3) Input the Factor of the Regression Equation. Press and input the

factors, press to confirm.

Method 2: Use Standard Samples

1) Set Fit Method. Press to set fit method, press , to choose fit

method, then press to confirm.

2) Set Wavelength. Press to go into wavelength setting interface, press

, to select measure method and press to confirm. Input the

value of the wavelength and press to confirm.

3) Blank. Put the Reference Sample in the light path and press to do blank.

4) Setup Standard Samples. Press to setup standard, input the concentrations of corresponding standard samples according the indication

and press to confirm. Users can use , to choose the value

you just input and press to delete, then input a new value, press

to confirm. Press to cancel after all the input.

5) Calibrate Standard Samples. Put the corresponding standard samples in the

light path as the screen indicates and press to measure. Then the Abs. value would appear in the corresponding table.

Load the Stored Curves

In the “Calibration Table” interface, press to go into files select interface. Use

, to select the curve you need and press to load. Users can press

to view the curve, press to cancel.

0 Conc. 4

4. Return the sample measurement interface

In the “Calibration Table” interface, press to return the Quantitative Test interface.

5. Blank

Put the Reference Sample in the light path, press to do blank.

6. Measure Samples

Place the sample to be tested in the light path, press to measure. Then the test result will display in the data sheet. Repeat this step to finish measuring all the samples.

7. Print the Test Results

Press to print the Test Results.

Quantitative Test Report

File Name: Abc Date & Time: mm-dd-yyyy, hh:mm:ss

No. 500.0nm Abs(eff) C(mg/L)

1 0.212 0.212 0.212 2 0.210 0.210 0.210 3 0.209 0.209 0.209

Fitting Params:

C=1.000*A^1

r=1.000 Model: UV-3100PC SN: UEFXXXXXXX Version: A1.176 VWR International bvba.

Wavelength Scan

1. Enter into Wavelength Scan

Main menu, press numeric key or , to choose “Wavelength Scan” and

press to enter.

0.000 Abs 1.200

2. Parameters Setup

Press to set parameters, set “Scan From” , “scan to”, “scan step” and “scan speed”,

press to confirm.

3. Set Photometric Mode

Press to set photometric mode，choose “T%”, “Abs.” or “E” and press to confirm.

4. Scan Baseline

Put Reference Sample in the light path, press to scan the baseline, press to cancel.

5. Scan Samples

Put the samples in the light path，press to scan the sample, press to cancel.

+ 63 hidden pages

VWR International 634-6002, UV-3100PC Instruction Manual

Specifications and Main Features

Frequently Asked Questions

User Manual