VistaLab Ovation Operator's Manual
Operator’s Guide for
0.2-10mL
0.2-10mL
Electronic Single Channel
and Macro Volume Pipettes
L065-0001-001 Rev. F
Introduction
TABLE OF CONTENTS
roduct Description & Specifications 1-1
P
ipette Components 1-2
P
BioNatural
Pipetting
Operating
Procedures
Calibration
Maintenance &
Troubleshooting
Introduction & Ergonomics 2-1
Ovation pipetting 2-3
Picking Up & Holding 2-3
Selecting Liquid Handling Functions 2-3
Acquiring Tips 2-3
Aspirating and Dispensing 2-4
Ejecting Tips 2-4
Using serological or volumetric pipettes (macro models) 2-5
Getting Started, Keypad & Display 3-1
Function Indicators and Setting Indicators 3-2
Set-Up of Liquid Handling Functions 3-2
Set-Up of Pipette Settings 3-9
Introduction 4-1
Determining a New Calibration Factor 4-1
Entering a Known Calibration Factor 4-2
Calibration with Serological or Volumetric pipets 4-2
Cleaning Exterior 5-1
Disinfecting Nozzles 5-1
Replacing a Nozzle (Single Channel) 5-1
Replacing a Nozzle (Macro 5mL and 10mL) 5-2
Replacing a Nozzle Filter (Single Channel) 5-3
Replacing a Nozzle Filter (Macro 5mL) 5-4
Replacing a Nozzle Filter (Macro 10mL) 5-5
Replacing a Nozzle Insert (Macro 10mL) 5-5
Battery Information 5-6
Troubleshooting 5-7
Appendix A
Appendix B
Ovation BioNatural Pipette – Electronic Single Channel Operator’s Guide
Warranty, Safety Compliance & Contact Information A-1
Hazards & Precautions A-2
Operating Temperature & Environment Conditions A-2
Power Supply and Battery Specifications A-2
Chemical Compatibility A-2
Tips & Accessories B-1
ELECTRONIC SINGLE CHANNEL
1
AND MACRO OVATION PIPETTES
Ovation BioNatural™pipettes are the ideal ergonomic solution for handling repetitive
and complex liquid handling tasks that contribute to repetitive stress injuries in
today's laboratory. Ovation pipettes increase laboratory efficiency through automated
liquid handling routines, while reducing fatigue and the effect of poor postures.
This manual describes how to use and care for your Ovation electronic single channel
or macro pipette. As you can see from its appearance, it is different from every
pipette that you have used before, therefore please read the instructions carefully.
Upon initial receipt of the pipette, the battery must be charged for 90 minutes prior to
use. (See instructions on page 5–5). Also, please activate the warranty on the VistaLab
Technologies web site – www.vistalab.com. The required on-line form can be found in
the “support” menu. Retain all packing materials in the event that the pipette is to be
sent back to VistaLab Technologies for calibration verification or service.
Product Description
Electronic single channel and macro Ovation BioNatural Pipettes are adjustable volume,
air displacement,
fully electronic motor-driven
dispense precise fluid volumes.
Catalog# Color Range Increments Accuracy* Precision*
20L
10L 0.50% at 10L
1.80% at 2L
125L
62.5L 0.24% at 62.5L
12.5L 1.00% at 12.5L
250L
125L 0.25% at 125L
25L 1.00% at 25L
1250L
625L 0.20% at 625L
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
1065-0020
1065-0020L
1065-0125
1065-0125L
1065-0250
1065-0250L
Note: Catalog numbers ending in “L” are left-handed models –
1065-1250
the LCD screen is on the opposite side of the pipette.
Yello
Gree
Blue
Purp
Grey
Red
Volume Dispensing
pipettes intended to aspirate and
–1.0% at 20L 0.30% at
0.5 — 20L 0.05L –1.5% at
–3.0% at 2L
–0.8% at 125L 0.15% at
2 — 125L 0.5L –0.8% at
–2.9% at
–0.8% at 250L 0.15% at
5 — 250L 1L –0.8% at
–2.5% at
–0.75% at 1250L 0.15% at
25 — 1250L 5L –0.8% at
1 - 1
Pipette Components
100-
0.2-10mL
Tip Eject
Ejector
Pipetting
Adjustabl
e
Bod
Nozzle
Keypa
Recharging Port
Volume
Description Function
Pipetting Trigger Initiates pipetting action
Tip Eject Button Ejects disposable pipette tips with minimal thumb force
LCD
Captive
Serial
Adjustable Hook Accommodates left and right handed users
Ejector Sleeve Stored energy from tip installation releases tips
Nozzle T
Body Larger grip surface for comfort, eliminating tight hand grip
Keypad Selects and sets up liquid handling functions, settings and calibration
LCD
Battery Lithium ion rechargeable, located in base of pipette (not shown)
Volume Label Identifies volume range
Recharging Port Power supply connection for battery recharging
Power Supply AC adapter for recharging battery (not shown)
Captive Screw When loosened, allows access to inside of pipette for battery replacement
Serial Number Provides positive identification for the pipette
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
apered, chemically resistant coupling that secure pipette tips
Indicates liquid handling functions, status, settings,
and remaining battery power
1 - 2
BIONATURAL PIPETTING
2
Introduction
Posture
Studies have shown that pipetting is the #1 cause of musculoskeletal disorders in the
laboratory – it is, by nature, a repetitive process that puts strain on the body. While
we can’t change how repetitive pipetting is, we can and did change how comfortable
you are while doing it.
The Ovation BioNatural Pipette is the only pipette designed to keep your hand in the
neutral position recommended by ergonomics experts. We call this BioNatural™
pipetting – it allows a lower hand location to ease stress in the shoulder, and a
relaxed wrist angle eliminates uncomfortable extension and radial deviation
movements in the arm. Force, velocity and exertion from repetition or duration have
also been neutralized because of the Ovation pipette’s unique working position and
reduced forces required during operation.
Some practice may be required to change years of posture and habits developed
using standard axial pipettes; the physical benefits of BioNatural pipetting are worth
the practice! When using the Ovation pipette, arm/hand elevation should remain low
to the work surface, wrist rotation should not exceed 90º, and hand posture should
remain relaxed with the wrist and back of hand slightly flexed.
Arm elevation remains low,
minimizing stress to elbow,
shoulder and neck
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
A loose, relaxed grip increases
available strength in the hand,
improving endurance and
productivity during pipetting
Wrist remains in a neutral
range of motion throughout
all pipetting operations
2 - 1
Keep a “comfort zone”
for pipetting
Maximum
Reach
“Comfort Zone”
14”
24”
14”
24”
Ergonomics experts recommend establishing a “comfort zone” of
movement for the task of pipetting. Position your body within 9” of the
counter surface, and keep the items needed within easy reach. Ideally this
should be 14” or less, and should not exceed 24” of occasional reaching.
Wrist rotation should not exceed 90˚ from the work surface, and arm/hand
elevations should remain low to the counter surface.
1. Relaxed shoulders
and upper arms
2. Low thumb forces
3. Relaxed hand grip
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
4. Neutral forearm
and wrist rotation
5. Low arm and
elbow height
Ovation comfort-zone benefits:
1. Minimized tension and fatigue
2. Minimized exertion and contact stress
3. Low contact pressure
4. Lowest pressure in carpal tunnel and to median nerve
5. Minimized tension to shoulder and elbow
2 - 2
icking Up the Pipette
P
Selecting Liquid
Handling Functions
and Changing
Settings
The Ovation pipette has been designed
o allow the body of the pipette to fill
t
your palm. Rotate the adjustable
rest comfortably on your
texture of the unit’s back
hook to
forefinger. The
helps reinforce
correct hand positioning.
The unique ergonomic design and
adjustable hook is compatible for both
right and left-handed use.
Keep hand posture relaxed – there’s no
need for a “firm grip” when using an
Ovation pipette.
The Ovation pipette’s keypad is easy to
use and provides access to all liquid
handling functions and settings. Simply
press the buttons on the keypad,
monitoring the selection of functions or
changes to settings on the LCD display.
Acquiring Tips
Complete information and examples of
all liquid handling functions and
settings are included in “Operating
Procedures” - Chapter 3 of this guide.
While maintaining a flat wrist-hand
posture, insert the Ovation pipette
nozzles into a tip. Press down until you
hear or feel a “click”. This “click”
indicates that the tip is properly seated
and ready for use.
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
2 - 3
Aspirating and
Dispensing
Smooth gliding movements of the upper
extremities with proper posture and
minimal stress is the key to ergonomically
orrect pipetting. Maintain a low forearm
c
elevation and neutral wrist posture
throughout the pipetting cycle. With
Electronic Single Channel models, Immerse
pipette tip to an appropriate depth (1mm
for 0.5–20µL, 2 to 3mm for 2µL–125µL & 5-
50µL, and 2 to 4mm for 25–1250µL).
2
Press
& release the pipetting trigger to aspirate
sample. Press pipetting trigger again to
dispense or mix sample.
Aspirating and dispensing large sample
volumes traditionally create ergonomic
challenges because of an axial pipette’s
design, elongated macro tips, or the
overall length of typical serological pipets
The Ovation macro pipette allows the
operator to minimize wrist rotation
and
arm elevation.
.
Ejecting Tips
Immerse pipette tip into sample, then
press and release the pipetting trigger to
aspirate. Wait one second after beep
tone, then remove tip and place it into
dispensing container at same angle
used during aspiration. Press pipetting
trigger again to dispense or mix sample.
Note: To interrupt aspirating cycle and/or
prevent accidental overfilling of a pipet (as
when set volume exceeds capacity of
serological pipet), quickly press and release
pipetting trigger. PURGE will be on
display. Pressing pipetting trigger again
then expels any liquid or air bubbles in the
tip or pipet.
Ovation pipettes store energy captured
during the acquisition of a tip, and use
it to discard tip when the tip eject button
is pressed. Point pipette tip into a suitable
waste container and press the tip eject
button to discard the tip.
To minimize forearm elevation and
“reaching”, position the waste container
below worksurface level or use shallow
containers. Raising the arm to discard
tips into a tall receptical on the counter
may negate some of the benefits of
Ovation’s low-profile design.
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
2 - 4
Using serological
or volumetric
pipets
(macro models only)
Note: Due to the
additional length of the air
olumn or variations in
c
markings on serological or
volumetric pipets, it may
be necessary to adjust the
Ovation’s calibration
factor. To optimize
performance, review the
calibration instructions in
chapter 4.
When extra length is needed to reach
the bottom of tall vessels, glass or
lastic serological or volumetric pipets
p
can be used with Ovation macro
volume models.
The nozzle on the 0.2-10mL model
accommodates both tips and pipets.
nsert the end of a pipet into the nozzle
I
until it stops. To remove the pipet,
manually pull it out of the nozzle.
The 100-5000mL model requires
installation of a Sero-Adapter prior to
use with serological pipets. The adapter
easily attaches to the pipette in place
its standard nozzle (See instructions
below).
Insert the end of a pipet into the
Sero-Adapter until it stops. To remove
the pipet, manually pull it out of the
Sero-Adapter. Re-install the standard
nozzle to use pipette tips.
of
Installing a
Sero-Adapter
(100-5000µL model only)
1.
Place a macro pipette tip onto the nozzle and push
on ejector sleeve until click is heard. Grip tip firmly
near the nozzle, and turn it in a counterclockwise
direction to unscrew and remove nozzle from
pipette. Store nozzle in a safe place.
2.
Carefully screw on adapter (clockwise). Ensure
adapter is square to threads for proper installation.
Nozzle
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
2 - 5
OPERATING PROCEDURES
3
etting Started
G
Pipette Keypad
he general steps to set up and operate an electronic single channel or macro model
T
Ovation pipette are:
1. Select a liquid handling function
2. Review and/or change settings as required
3. Acquire tip
4. Run the liquid handling function according to its protocol
5. Eject disposable tip into a proper waste container.
Familiarize yourself with the use of the keypad, display prompts, set-up and use of
the pipette’s liquid handling functions in this chapter before use.
Button Description
Advances display to next liquid handling function in the run
mode. When in set-up, the button advances display to
the next
be changed.
setting within the liquid handling function that can
When changing the setting(s) within a liquid handling
UP DOWN
Pipette Display
Displays volume and values
during set-up, e.g. # of mix
example
Ovation BioNatural Pipette – Electronic Single Channel and Macro Operator’s Guide
cycles, # of multiple dispenses
Displayed when cal factor
been changed from factory
setting of 1.000
function, pressing the or button adjusts the settings.
When pressed together and held, they are used to access
pipette settings.
Description
has
Icon indicates
remaining
battery capacity
Symbol indicates next
portion of aspirate (up) or
dispense (down) cycle to
be performed
Displays
function
that is active
liquid handling
, setting or prompt
3 - 1
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