Vernier Go Direct SpectroVis Plus, GDX-SVISPL User Manual
1
attached while collecting data
wirelessly.
3. Turn on the spectrometer by
pressing the power button once. The
Bluetooth®LED will blink.
4. Launch Spectral Analysis 4.
5. Click or tap Connect a
Spectrometer.
6. Click or tap your Go Direct
SpectroVis Plus from the list of
Discovered Wireless Devices. Your
spectrometer's ID is located near the
barcode on the label. The Bluetooth
LED on the sensor will now glow
blue (no longer flashing).
7. Click or tap Done to enter datacollection mode. You are now ready
to continue your experiment.
Spectral Analysis 4
l Chromebook: SpectralAnalysis 4
l LabQuest: LabQuest App
l Mobile Device:
SpectralAnalysis 4
4. The software will identify the
spectrometer and enter datacollection mode. You are now
ready to continue your experiment.
Charging the Sensor
Connect Go Direct SpectroVis Plus to the included AC power cable for at least
eight hours. Note: This is only required for data collection via Bluetooth
wireless technology.
Charging
Orange LED next to battery icon is solid while
sensor is charging.
Fully charged
Green LED next to battery icon is solid when
sensor is fully charged.
Powering the Sensor
Turning on the sensor
Press Power button once or connect to USB.
Green LED indicator next to Power icon is solid
when unit is on.
Putting the sensor in sleep
mode
Press and hold button for more than three
seconds to put into sleep mode. Green LED
indicator turns off when sleeping.
Connecting the Sensor
See the following link for up-to-date connection information:
www.vernier.com/start/gdx-svispl
Go Direct
®
SpectroVis®Plus
(Order Code GDX-SVISPL)
Go Direct SpectroVis Plus is a portable, visible to
near-IR spectrophotometer and fluorometer. This
spectrophotometer can be used in a wide range of
introductory spectroscopy experiments for chemistry, biology, and physics. Such
experiments include: determining the peak wavelength to collect data on
solution concentration for Beer’s law studies, collecting a full wavelength
spectrum to measure absorbance, percent transmittance, fluorescence, or
emissions, and monitoring rates of reaction.
Note: Vernier products are designed for educational use. Our products are not
designed nor are they recommended for any industrial, medical, or commercial
process such as life support, patient diagnosis, control of a manufacturing
process, or industrial testing of any kind.
What's Included
l Go Direct SpectroVis Plus
l 15 plastic cuvettes and lids
l Rechargeable battery (included inside unit; remove if data collection is
strictly via USB)
l Micro USBcable
l Power supply (for battery recharge only; do not plug in if taking data)
Compatible Software
See www.vernier.com/manuals/gdx- svispl for a list of software compatible with Go
Direct SpectroVis Plus.
Getting Started
Please see the following link for platform-specific connection information:
www.vernier.com/start/gdx-svispl
Bluetooth Connection USB Connection
1. Install Spectral Analysis™ 4 on
your computer, Chromebook™, or
mobile device. See
www.vernier.com/spectral-analysis
for software availability.
2. Charge your spectrometer for at least
eight hours before first use using the
included pow er supply. Disconnect
the power supply before attempting
to collect data. It is recommended
that you not have the power supply
1. Do not connect the power supply.
You do not need it when taking
data via USB. Note: If collecting
data via USB, it is suggested you
remove the battery from the
spectrometer.
2. Connect the spectrometer to the
USB port.
3. Launch the software. Options
include
l Computer: Logger Pro or
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3. Fill a cuvette about 3/4 full with distilled water (or the solvent being used
in the experiment) to serve as the blank. After the Spectrophotometer has
warmed up, place the blank cuvette in the Spectrophotometer. Align the
cuvette so the clear side of the cuvette is facing the light source. Click or tap
Finish Calibration.
4. You are now ready to collect data. Fill a cuvette about 3/4 full of a sample
of the solution to be tested. Place the sample in the Spectrophotometer and
click Collect. Click Stop to end data collection. The spectrum is
automatically stored.
Measurement vs. Concentration (Beer's law)
1. Select Measurement vs. Concentration.
2. The calibration dialog box will appear. The warm-up timer starts counting
when the spectrometer connects to the platform. As a result, the countdown
may appear to vary. The minimum warm-up time is 90 seconds. For best
results, we suggest a warm-up time of several minutes.
3. Fill a cuvette about 3/4 full with distilled water (or the solvent being used
in the experiment) to serve as the blank. After the Spectrophotometer has
warmed up, place the blank cuvette in the Spectrophotometer. Align the
cuvette so the clear side of the cuvette is facing the light source. Click or tap
Finish Calibration.
4. Follow the instructions in the Choose a Wavelength dialog box or simply
type in the wavelength you wish to measure. Select Done.
5. Click Collect. Your first sample should still be in the Spectrophotometer.
After the reading stabilizes, click Keep. Enter the concentration of the
sample and click or tap Keep Point.
6. Place your second sample in the cuvette slot. After the reading stabilizes,
click Keep. Enter the concentration of the sample and click or tap Keep
Point.
7. Repeat Step 6 for the remaining samples. W hen finished, click Stop to end
data collection. The data is automatically stored.
8. To see the best fit line equation for the standard solutions, click Graph
Tools, select Apply Curve Fit, and choose Linear. Click or tap Apply.
9. If doing Beer's law to determine the concentration of an unknown, place the
unknown sample in the cuvette holder. Click or tap Graph Tools and enable
Interpolate. Click or tap along the line until you find the concentration
value that matches your unknown's measurement.
10. Save or export your data from the File menu.
Measurement vs. Time (Kinetics)
1. Select Measurement vs. Time.
2. The calibration dialog box will appear. The warm-up timer starts counting
when the Spectrophotometer connects to the platform. As a result, the
countdown may appear to vary. The minimum warm-up time is 90 seconds.
For best results, we suggest a warm-up time of several minutes.
3. Fill a cuvette about 3/4 full with distilled water (or the solvent being used
in the experiment) to serve as the blank. After the Spectrophotometer has
Connecting via Bluetooth
Ready to connect Press Power button once. Blue LED next to
Bluetooth icon flashes when sensor is ready to
connect.
Connected Blue LED next to Bluetooth icon is solid when
sensor is connected via Bluetooth wireless technology.
Connecting via USB
Connected and charging Orange LED next to battery icon is solid when
sensor is connected to Spectral Analysis via
USB. LED next to Bluetooth icon is off.
LEDnext to power is solid green. LED next to
USB icon is solid green.
Connected, fully charged LED next to battery icon is off when sensor is
connected to Spectral Analysis via USB and
fully charged. LED next to Bluetooth icon is off.
Charging via USB, connected via Bluetooth
This combination is not permitted.
Charging via AC power,
connected via Bluetooth
This combination is not recommended.
Using the Product with Spectral Analysis 4
Connect the sensor following the steps in the Getting Started section of this user
manual.
Collect Data with Spectral Analysis 4
The three options for Experiment Types are:
1. Measurement vs. Wavelength—collect a full spectrum.
2. Measurement vs. Concentration—conduct a Beer's law experiment.
3. Measurement vs. Time—collect time-based data for a kinetics experiment.
By default, Absorbance is selected. If you want to measure %Transmittance, use
the toggle switch. Note: Visit www.vernier.com/spectral- analysis for status of
intensity and fluorescence support.
Measurement vs. Wavelength (Full Spectrum)
1. Select Measurement vs. Wavelength.
2. The calibration dialog box will appear. The warm-up timer starts counting
when the Spectrophotometer connects to the platform. As a result, the
countdown may appear to vary. The minimum warm-up time is 90 seconds.
For best results, we suggest a warm-up time of several minutes.
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the Spectrophotometer to warm up for a minimum of five minutes.
2. Fill a cuvette about 3/4 full with distilled water (or the solvent being used
in the experiment) to serve as the blank. After the Spectrophotometer has
warmed up, place the blank cuvette in the Spectrophotometer. Align the
cuvette so the clear side of the cuvette is facing the light source.
3. Follow the instructions in the dialog box to complete the calibration, and
then click .
Collect Data with Logger Pro
There are three general types of data collection that measure absorbance or
transmittance—absorbance (or %T) vs. wavelength, which produces a spectrum,
absorbance (or %T) vs. concentration for Beer’s law experiments, and absorbance
(or %T) vs. time for kinetics experiments.
Measurement vs. Wavelength (Generate a Spectrum)
1. Fill a cuvette about 3/4 full of a sample of the solution to be tested. Place
the sample in the Spectrophotometer and click . Click to end
data collection.
2. To store the spectrum data, choose Store Latest Run from the Experiment
menu.
Measurement vs. Concentration (Beer’s Law Studies)
1. Generate a spectrum as described above.
2.
Click the Configure Spectrophotometer Data Collection button, .
There are three regions in this box:
l Collection Mode The three options for data collection are offered. If the
measurement (Absorbance in this example) vs. Time or vs. Concentration is
selected, a wavelength or wavelengths will need to be chosen.
l Graph The graph displays a full-spectrum analysis of the sample in the
cuvette holder. By default, the wavelength with the maximum measured
value will be selected. You may wish to select a different wavelength. See
Step 3 for details.
l List of wavelength options This column lists all the available wavelengths.
It becomes active when either the Concentration or Time mode is selected.
warmed up, place the blank cuvette in the Spectrophotometer. Align the
cuvette so the clear side of the cuvette is facing the light source. Click or tap
Finish Calibration.
4. Follow the instructions in the Choose a Wavelength dialog box or simply
type in the wavelength you wish to measure. Select Done.
5. The default data-collection settings collect measurements every two seconds
(USB) or three seconds (BLE) until the user manually stops data collection.
6. Mix the reactants. Transfer ~2 mL of the reaction mixture to a cuvette and
place the cuvette in the spectrometer. Click or tap Collect.
7. When finished, click or tap Stop.
8. To fit a function to the data, click Graph Tools, select Apply Curve Fit, and
choose the appropriate curve fit. Click or tap Apply.
9. To add a calculated column to the data set, click OK in the measurement
header on the data table. Select Add Calculated Column. Modify the name,
units and displayed precision accordingly. Select Insert Expression and
select the appropriate equation. Modify the parameters and column options,
if necessary. Click or tap Apply. The calculated column is automatically
displayed on the graph.
10. Save or export your data from the File menu.
Change the Settings in Spectral Analysis
1. Click or tap the gear to show the Spectrometer Settings dialog.
2. There are three parameters listed in the dialog box:
l Integration Time: This is similar to the shutter speed of a camera.
Spectral Analysis 4 automatically selects the proper sample time during
calibration.
l Wavelength Smoothing: This is the number of adjacent readings on
either side of a given value that is used to calculate an average value.
l Temporal Averaging: This is the number of readings taken at a given
wavelength to calculate an average reading.
3. Select the Calibrate button to recalibrate your spectrometer at any time.
Using the Product with Logger Pro
Connect the sensor following the steps in the Getting Started section of this user
manual.
Select the Type of Data (or Units) You Want to Measure
The default data type is absorbance. If you want to measure the absorbance of a
solution, proceed directly to the Calibrate section below.
If you want to measure %T, fluorescence (excited at 405 nm or 500 nm), or
intensity, do the following:
1. Choose Change Units ► Spectrophotometer from the Experiment menu.
2. Select the unit or data type you wish to measure.
Calibrate (Not Required if Measuring Intensity or Fluorescence)
1. To calibrate Go Direct SpectroVis Plus, choose Calibrate ►
Spectrophotometer from the Experiment menu. Note: For best results, allow
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