Vernier GDX-O2 User's Guide

®
Go Direct O2Gas
(Order Code GDX-O2)
Go Direct O2Gas measures gaseous oxygen concentration and air temperature. This sensor has a wide measurement range, which is ideal for studying human and cellular respiration. A 250 mL Nalgene bottle is included for running controlled experiments with small plants and animals.
Go Direct O2Gas can be used in a variety of experiments:
l Test catalase activity under various conditions. l Measure oxygen consumption at rest and after exercise. l Measure the change in O l Compare the rates of cell respiration in germinating and non-germinating
peas.
Note: Vernier products are designed for educational use. Our products are not designed nor are they recommended for any industrial, medical, or commercial process such as life support, patient diagnosis, control of a manufacturing process, or industrial testing of any kind.

What's Included

l Go Direct O l Micro USBCable l 250 mL Nalgene Bottle w/Lid
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Gas

Compatible Software

See www.vernier.com/manuals/gdx-o2 for a list of software compatible with GoDirectO2Gas.

Getting Started

Please see the following link for platform-specific connection information:
Bluetooth Connection USB Connection
1. Install Vernier Graphical Analysis™ on your computer, Chromebook™, or mobile device. If using LabQuest®, make sure LabQuest App is up to date. See www.vernier.com/ga4 for Graphical Analysis availability or www.vernier.com/downloads to update LabQuest App.
2. Charge your sensor for at least 2hours before first use.
gas produced during photosynthesis.
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www.vernier.com/start/gdx- o2
1. If using a computer or Chromebook, install Vernier Graphical Analysis. If using LabQuest, make sure LabQuest App is up to date. See www.vernier.com/ga4 for Graphical Analysis availability or www.vernier.com/downloads to update LabQuest App.
2. Connect the sensor to the USB port.
3. Turn on your sensor by pressing the power button once. The LED will blink red.
4. Launch Graphical Analysis or turn on LabQuest.
5. If using Graphical Analysis, click
3. Launch Graphical Analysis or turn on LabQuest. You are now ready to collect data.
4. This is a multi-channel sensor. To change the channel selections, see www.vernier.com/start/gdx-o2
or tap Sensor Data Collection. If using LabQuest, choose Wireless Device Setup > Go Direct from the Sensors menu.
6. Select your Go Direct sensor from
Note: This sensor does not work with the original LabQuest. It works with
LabQuest 2 or LabQuest 3. the list of Discovered Wireless Devices. Your sensor's ID is located near the barcode on the sensor. The LED will blink green when it is successfully connected.
7. Click or tap Done. You are now ready to collect data.
8. This is a multi-channel sensor. To change the channel selections, see www.vernier.com/start/gdx-o2
Charging the Sensor
Connect Go Direct O2Gas to the included Micro USB Cable and any USB device for two hours.
You can also charge up to eight Go Direct O2Gas Sensors using our Go Direct Charge Station, sold separately (order code: GDX-CRG). An LED on each GoDirectO2Gas indicates charging status.
Charging
Blue LED on steady while sensor is connected to the Micro USB Cable or Charge Station.
Fully charged
Blue LED is off when charging is complete.
Powering the Sensor
Turning on the sensor
Putting the sensor in sleep mode
Press button once. Red LED indicator flashes when unit is on.
Press and hold button for more than three seconds to put into sleep mode. Red LED indicator stops flashing when sleeping.
Connecting the Sensor
See the following link for up-to-date connection information:
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www.vernier.com/start/gdx- o2
Connected and charging Blue and Green LED solid when sensor is con-
nected to Graphical Analysis via USB and unit is charging. (Green LED is obscured by the blue one.)
Connected, fully charged Green LED solid when sensor is connected to
Graphical Analysis via USB and the unit is fully charged.
Charging via USB, connected via Bluetooth
Blue LED is solid and green LED is flashing, but the green flashing LED looks white because it is overwhelmed by the blue.

Identifying the Sensor

When two or more sensors are connected, the sensors can be identified by tapping or clicking Identify in Sensor Information.

Using the Product

Connect the sensor following the steps in the Getting Started section of the user manual.
Note: For best results, keep the sensor upright as much as possible during use. Reading may drift if used in a horizontal position.

Channels

Go Direct O2Gas has three measurement channels:
l O
Gas
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l O
Gas - rTC
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l Temperature

O2Gas

This channel measures the oxygen concentration in the air. Units can be changed from percent (%) to parts per thousand (ppt), parts per million (ppm), or or mg/m3. See www.vernier.com/til/3846 for more information. There is built-in temperature compensation on this channel that is most effective for slow temperature changes. For rapid temperature changes, consider using the O2Gas­rTCchannel. O2Gas is the default channel that is active when the sensor is connected.

O2Gas - rTC

This channel measures oxygen gas concentration, but in addition to the built-in temperature compensation, it uses the on-board thermistor to apply a rapid temperature correction to the oxygen gas reading. This channel should only be used in experiments where starting conditions will be at room temperature and where rapid changes in temperature are likely to occur. Units can be changed from percent (%) to parts per thousand (ppt), parts per million (ppm), or mg/m3.
See www.vernier.com/til/3846 for more information. This channel is not active by default when then the sensor is connected.

Temperature

This channel measures air temperature and is used for rapid temperature compensation. Units can be changed from °C to °F or K. This channel is not active by default when the sensor is connected.

Videos

View videos related to this product at www.vernier.com/gdx-o2

Calibrating the Sensor

O2Gas

The sensor is factory calibrated. For many experiments, it will not be necessary to calibrate. However, for best accuracy, the sensor can be calibrated using a one-point calibration using a known concentration of atmospheric oxygen. Enter a value of 20.9% oxygen or a corrected value from the table in the Effect of Humidity section of this user manual. Once finished, the sensor should read
20.9% (or the value entered in the table).

O2Gas - rTC

The channel is factory calibrated. For many experiments, it will not be necessary to calibrate the sensor when using this channel. However, for more accurate measurements, the channel can be calibrated using the instructions in the O2Gas section of this user manual.
Temperature
The temperature sensor is factory calibrated and cannot be calibrated by the user.

Effect of Humidity

Because the oxygen concentration varies with the amount of water vapor in the atmosphere, you may want to adjust your atmospheric oxygen calibration value to improve accuracy when using the sensor. The accepted value of 20.9% for atmospheric oxygen levels is calculated in dry air (0% relative humidity). If you know the relative humidity of the location at which you are calibrating, you can substitute one of the values in the table below in place of 20.9%. You can also use the formula below to calculate the corrected oxygen level for calibration. Oxygen concentration decreases linearly as humidity increases. The formula that describes this relationship is
y = –0.008x + 20.9, where y = corrected oxygen concentration and x = measured relative humidity.
Relative humidity (%) 0 25 50 75 100
Oxygen (%) 20.9 20.7 20.5 20.3 20.1
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Specifications

Oxygen Sensor
Type Electrochemical cell
Range 0–100% (0–1000 ppt) O
Accuracy (at standard
±1% O
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pressure 760mmHg)
Resolution 0.01% O
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Response time ~12 seconds to 90% of final value
Warm-up time Less than 5 seconds to 90% of final value
Pressure effect Directly proportional
Pressure range: 0.5 atm to 1.5 atm
Gas sampling mode Diffusion
Temperature Sensor
Type Thermistor
Accuracy ±0.5°C
Resolution 0.1°C
Wireless specification Bluetooth 4.2
USB specification 2.0
Maximum wireless range 30 m unobstructed
Battery 650 mA
Battery life (single full charge) ~8 hours
Battery life (long term) ~500 full charge cycles (several years
depending on usage)
Normal operating temperature range
20 to 40°C (above 35°C will shorten battery life)
Operating humidity range 0 to 95% RH
Dimensions Sensor tube: 38 mm length, 28 mm OD;
total length: 155 mm
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Care and Maintenance

Battery Information

Go Direct O2Gas contains a small lithium-ion battery. The system is designed to consume very little power and not put heavy demands on the battery. Although the battery is warranted for one year, the expected battery life should be several years. Replacement batteries are available from Vernier (ordercode:GDX-BAT-650).

Storage and Maintenance

Important: Go Direct O2Gas must be stored upright when not in use. Failure to store upright will shorten the life of the sensor and void the warranty.
To store Go Direct O2Gas for extended periods of time, put the device in sleep mode by holding the button down for at least three seconds. The red LED will stop flashing to show that the unit is in sleep mode. Over several months, the battery will discharge but will not be damaged. After such storage, charge the device for a few hours, and the unit will be ready for use.
Exposing the sensor to temperatures below –15°C or over 45°C will damage the sensor. Additionally, temperatures over 35°C (95°F) will reduce the battery's lifespan. If possible, store the device in an area that is not exposed to temperature extremes.
Note: It is recommended that you wash the Nalgene bottle by hand. Most dishwashers will melt plastic bottles.

Water Resistance

Important: Go Direct O2Gas is neither waterproof, nor is it water resistant and should never be immersed in water.
If any liquid gets into the device, immediately power the unit down (press and hold the power button for more than three seconds). Disconnect the sensor and charging cable, and remove the battery. Allow the device to dry thoroughly before attempting to use the device again. Do not attempt to dry using an external heat source.
Note: Damage due to liquids is not covered under warranty.

How the Sensor Works

Go Direct O2Gas measures the oxygen concentration in the range of 0 to 100% using an electrochemical cell. The cell contains a lead anode and a gold cathode immersed in an electrolyte. Oxygen molecules entering the cell are electrochemically reduced at the gold cathode. This electrochemical reaction generates a current that is proportional to the oxygen concentration between the electrodes. A thermistor inside the electrochemical cells adjusts the output current to control for slow changes in cell temperature. An amplifier converts the current to a digital signal.
The sensor also measures air temperature using a thermistor located in the base of the sensor shaft. When the temperature increases, the resistance of the
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thermistor decreases. A circuit in the sensor measures the resistance value at a particular temperature and converts the resistance using the Steinhart-Hart equation. Our programs perform this conversion to provide readings in °C (or other units). This thermistor is also used to adjust the output of the O2Gas - rTC channel, providing rapid temperature compensated measurements of oxygen gas concentration.

Troubleshooting

l Very important: Do not place the sensor into any liquid. The sensor is
intended only for measuring gaseous, not aqueous, O2concentration.
l Important: Go Direct O
Gas must be stored upright when not in use.
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Failure to store upright will shorten the life of the sensor and void the warranty.
l The sensor is sensitive to high humidity. Do not use in a condensing
environment.
l The temperature sensor is very sensitive. Holding the sensor in your hand
can cause an increase in temperature readings and change the O2gas value on the O2- rTC channel. For best results, hold the sensor by its base, close to the charging port.
l The sensor has built-in temperature compensation but the sensor can still be
sensitive to rapid and/or large changes in temperature. The sensor is designed to operate best between 20 and 40°C. This does not prohibit the sensor from being used in temperatures outside this range. Just allow enough time for the sensor to stabilize at the desired air temperature. For more accurate results, the sensor can be calibrated at the temperature used.
l The recommended sampling rate is 1 sample per second or slower for this
sensor. This allows the sensor to average a set of measurements before reporting a value. Gas must diffuse through the holes in the sensor tube before the sensor detects any changes in concentration. Because diffusion of gases is a slow process, there can be a delay in response time.
l To collect data in a controlled environment, we recommend that you use the
250 mL Nalgene collection bottle that is included with your sensor. Place the tip of the sensor into the opening of the bottle and push the sensor into the bottle.
l To collect data in a controlled environment and also use our one of our CO
gas sensors, we recommend purchasing either the BioChamber 250 (250 mL capacity) or the BioChamber 2000 (2 L capacity). Each BioChamber has a second, grommetted opening so that you can insert two probes at once.
For troubleshooting and FAQs, see www.vernier.com/til/4127

Repair Information

If you have followed the troubleshooting steps and are still having trouble with your Go Direct O2Gas, contact Vernier Technical Support at support@vernier.com or call 888-837-6437. Support specialists will work with you to determine if the unit needs to be sent in for repair. At that time, a Return
Merchandise Authorization (RMA) number will be issued and instructions will be communicated on how to return the unit for repair.

Accessories/Replacements

Item Order Code
Micro USB Cable
USB-C to Micro USBCable
Go Direct 650 mAh Replacement Battery
BioChamber 250
BioChamber 2000
250 mL Nalgene Bo ttle w/Lid
CB-USB-MICRO CB-USB-C-MICRO GDX-BAT-650 BC-250 BC-2000 CO2-BTL

Warranty

Warranty information for this product can be found on the Support tab at
www.vernier.com/gdx- o2
General warranty information can be found at www.vernier.com/warranty

Disposal

When disposing of this electronic product, do not treat it as household waste. Its disposal is subject to regulations that vary by country and region. This item should be given to an applicable collection point for the recycling of electrical and electronic equipment. By ensuring that this product is disposed of correctly, you help prevent potential negative consequences on human health or on the environment. The recycling of materials will help to conserve natural resources. For more detailed information about recycling this product, contact your local city office or your disposal service.
Battery recycling information is available at www.call2recycle.org
Do not puncture or expose the battery to excessive heat or flame.
The symbol, shown here, indicates that this product must not be disposed of
in a standard waste container.

Federal Communication Commission Interference Statement

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This equipment has been tes ted and found to comply with the limi ts for a Class B digital devi ce, pursuant to Part 15 of the FCC rules. These l imits are desi gned to provide reasonable protection against harmful i nterference in a residential instal lation. This equipment generates, uses and can radiate radio frequency energy and, if not instal led and used in accordance with the i nstructions, may caus e harmful i nterference to radio communic ations. However, there is no guarantee that i nterference will not occur in a particular i nstall ation. If this equipment does cause harmful interference to radio or televisi on reception, which can be determined by turning the equipment off and on, the user is encouraged to try to correct the i nterference by one or more of the following measures:
Reorient or relocate the receivi ng antenna.
Increase the s eparation between the equipment and receiver.
Connect the equipment into an outlet on a ci rcuit different from that to whic h the recei ver is connected.
Consult the dealer or an ex perienced radio/TV technici an for help.
FCC Caution
This device c omplies with Part 15 of the FCC Rules. Operation is s ubject to the following two c onditions:
(1) this devi ce may not cause harmful interference and
(2) this devi ce must accept any i nterference received, includi ng interference that may c ause undesired operation
RF Expos ure Warning
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The equipment compli es with RF exposure limi ts set forth for an uncontrolled env ironment. The antenna(s) used for this transmitter must not be c o-located or operating i n conjuncti on with any other antenna or transmitter. You are cautioned that c hanges or modific ations not expressly approved by the party responsi ble for c ompliance c ould void your authority to operate the equipment.

IC Statement

This device c omplies with Industry Canada l icens e-exempt RSS standard(s). Operation is s ubject to the following two c onditions :
(1) this devi ce may not cause interference, and
(2) this devi ce must accept any i nterference, includi ng interference that may c ause undesired operation of the devi ce.
Industry Canada - Class B This di gital apparatus does not ex ceed the Class B limi ts for radio noise emis sions from digital apparatus
as set out in the i nterference-causing equipment standard entitl ed “Digital Apparatus,” ICES-003 of Industry Canada. Operation is s ubject to the fol lowing two c onditions: (1) thi s devi ce may not cause interference, and
(2) this devi ce must accept any i nterference, includi ng interference that may c ause undesired operation of the devi ce.
To reduce potential radio interference to other users, the antenna type and its gain should be so chosen that the equivalent isotropical ly radiated power (e.i.r.p.) i s not more than that permitted for s ucces sful communic ation.
RF exposure warning: The equipment compli es with RF expos ure l imits s et forth for an uncontrolled envi ronment. The antenna(s) used
for this transmitter must not be co-located or operating in conjuncti on with any other antenna or transmitter.
Le présent appareil est conforme aux CNR d’ Industrie Canada applic ables aux appareils radio exempts de lic ence. L’expl oitation est autorisée aux deux conditions sui vantes :
(1) l’appareil ne doit pas produire de brouillage, et
(2) l’appareil doit ac cepter tout interférence radioélec trique, même si cela résulte à un brouill age susceptibl e d’en compromettre l e fonctionnement.
Cet appareil numérique respecte l es li mites de bruits radioélectriques applic ables aux appareils numériques de Classe B prescrites dans la norme sur le matériel interférant-brouill eur: “Appareils Numériques,” NMB-003 édic tée par indus trie Canada. L’utili sation est s oumise aux deux conditions s uivantes :
(1) cet appareil ne peut caus er d’ interférences, et
(2) cet appareil doit accepter toutes i nterférences, y c omprises cell es susc eptibles de provoquer un dis fonctionnement du di sposi tif.
Afin de réduire les i nterférences radio potentielles pour les autres utilis ateurs, l e type d’antenne et s on gain doivent être choi sie de tell e façon que l ’équiv alent de puissanc e isotrope émi s (e.i.r.p) n’est pas plus grand que celui permis pour une c ommunication établie.
Avertissement d ’exposition RF: L’équipement est conforme aux l imites d’ exposi tion aux RF établi es pour un envi ronnement non
supervisé. L’antenne (s) uti lis ée pour ce transmetteur ne doit pas être j umelés ou fonctionner en conj onction avec toute autre antenne ou transmetteur.
Note: This product is a sensi tive measurement dev ice. For best results , use the cables that were provided. Keep the devi ce away from
electromagnetic noise sources, s uch as microwaves, monitors, electric motors, and appliances.
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Vernier Softwar e & Technology
Toll Free (888) 837- 6437 • (503) 277-2299 • Fax (503) 277- 2440
info@vernier.com • www.vernier.com
Rev. 2/ 19/20 21
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