Vernier GDX-O2 User's Guide

®
Go Direct O2Gas
(Order Code GDX-O2)
Go Direct O2Gas measures gaseous oxygen concentration and air temperature. This sensor has a wide measurement range, which is ideal for studying human and cellular respiration. A 250 mL Nalgene bottle is included for running controlled experiments with small plants and animals.
Go Direct O2Gas can be used in a variety of experiments:
l Test catalase activity under various conditions. l Measure oxygen consumption at rest and after exercise. l Measure the change in O l Compare the rates of cell respiration in germinating and non-germinating
peas.
Note: Vernier products are designed for educational use. Our products are not designed nor are they recommended for any industrial, medical, or commercial process such as life support, patient diagnosis, control of a manufacturing process, or industrial testing of any kind.

What's Included

l Go Direct O l Micro USBCable l 250 mL Nalgene Bottle w/Lid
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Gas

Compatible Software

See www.vernier.com/manuals/gdx-o2 for a list of software compatible with GoDirectO2Gas.

Getting Started

Please see the following link for platform-specific connection information:
Bluetooth Connection USB Connection
1. Install Vernier Graphical Analysis™ on your computer, Chromebook™, or mobile device. If using LabQuest®, make sure LabQuest App is up to date. See www.vernier.com/ga4 for Graphical Analysis availability or www.vernier.com/downloads to update LabQuest App.
2. Charge your sensor for at least 2hours before first use.
gas produced during photosynthesis.
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www.vernier.com/start/gdx- o2
1. If using a computer or Chromebook, install Vernier Graphical Analysis. If using LabQuest, make sure LabQuest App is up to date. See www.vernier.com/ga4 for Graphical Analysis availability or www.vernier.com/downloads to update LabQuest App.
2. Connect the sensor to the USB port.
3. Turn on your sensor by pressing the power button once. The LED will blink red.
4. Launch Graphical Analysis or turn on LabQuest.
5. If using Graphical Analysis, click
3. Launch Graphical Analysis or turn on LabQuest. You are now ready to collect data.
4. This is a multi-channel sensor. To change the channel selections, see www.vernier.com/start/gdx-o2
or tap Sensor Data Collection. If using LabQuest, choose Wireless Device Setup > Go Direct from the Sensors menu.
6. Select your Go Direct sensor from
Note: This sensor does not work with the original LabQuest. It works with
LabQuest 2 or LabQuest 3. the list of Discovered Wireless Devices. Your sensor's ID is located near the barcode on the sensor. The LED will blink green when it is successfully connected.
7. Click or tap Done. You are now ready to collect data.
8. This is a multi-channel sensor. To change the channel selections, see www.vernier.com/start/gdx-o2
Charging the Sensor
Connect Go Direct O2Gas to the included Micro USB Cable and any USB device for two hours.
You can also charge up to eight Go Direct O2Gas Sensors using our Go Direct Charge Station, sold separately (order code: GDX-CRG). An LED on each GoDirectO2Gas indicates charging status.
Charging
Blue LED on steady while sensor is connected to the Micro USB Cable or Charge Station.
Fully charged
Blue LED is off when charging is complete.
Powering the Sensor
Turning on the sensor
Putting the sensor in sleep mode
Press button once. Red LED indicator flashes when unit is on.
Press and hold button for more than three seconds to put into sleep mode. Red LED indicator stops flashing when sleeping.
Connecting the Sensor
See the following link for up-to-date connection information:
1
www.vernier.com/start/gdx- o2
Connected and charging Blue and Green LED solid when sensor is con-
nected to Graphical Analysis via USB and unit is charging. (Green LED is obscured by the blue one.)
Connected, fully charged Green LED solid when sensor is connected to
Graphical Analysis via USB and the unit is fully charged.
Charging via USB, connected via Bluetooth
Blue LED is solid and green LED is flashing, but the green flashing LED looks white because it is overwhelmed by the blue.

Identifying the Sensor

When two or more sensors are connected, the sensors can be identified by tapping or clicking Identify in Sensor Information.

Using the Product

Connect the sensor following the steps in the Getting Started section of the user manual.
Note: For best results, keep the sensor upright as much as possible during use. Reading may drift if used in a horizontal position.

Channels

Go Direct O2Gas has three measurement channels:
l O
Gas
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l O
Gas - rTC
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l Temperature

O2Gas

This channel measures the oxygen concentration in the air. Units can be changed from percent (%) to parts per thousand (ppt), parts per million (ppm), or or mg/m3. See www.vernier.com/til/3846 for more information. There is built-in temperature compensation on this channel that is most effective for slow temperature changes. For rapid temperature changes, consider using the O2Gas­rTCchannel. O2Gas is the default channel that is active when the sensor is connected.

O2Gas - rTC

This channel measures oxygen gas concentration, but in addition to the built-in temperature compensation, it uses the on-board thermistor to apply a rapid temperature correction to the oxygen gas reading. This channel should only be used in experiments where starting conditions will be at room temperature and where rapid changes in temperature are likely to occur. Units can be changed from percent (%) to parts per thousand (ppt), parts per million (ppm), or mg/m3.
See www.vernier.com/til/3846 for more information. This channel is not active by default when then the sensor is connected.

Temperature

This channel measures air temperature and is used for rapid temperature compensation. Units can be changed from °C to °F or K. This channel is not active by default when the sensor is connected.

Videos

View videos related to this product at www.vernier.com/gdx-o2

Calibrating the Sensor

O2Gas

The sensor is factory calibrated. For many experiments, it will not be necessary to calibrate. However, for best accuracy, the sensor can be calibrated using a one-point calibration using a known concentration of atmospheric oxygen. Enter a value of 20.9% oxygen or a corrected value from the table in the Effect of Humidity section of this user manual. Once finished, the sensor should read
20.9% (or the value entered in the table).

O2Gas - rTC

The channel is factory calibrated. For many experiments, it will not be necessary to calibrate the sensor when using this channel. However, for more accurate measurements, the channel can be calibrated using the instructions in the O2Gas section of this user manual.
Temperature
The temperature sensor is factory calibrated and cannot be calibrated by the user.

Effect of Humidity

Because the oxygen concentration varies with the amount of water vapor in the atmosphere, you may want to adjust your atmospheric oxygen calibration value to improve accuracy when using the sensor. The accepted value of 20.9% for atmospheric oxygen levels is calculated in dry air (0% relative humidity). If you know the relative humidity of the location at which you are calibrating, you can substitute one of the values in the table below in place of 20.9%. You can also use the formula below to calculate the corrected oxygen level for calibration. Oxygen concentration decreases linearly as humidity increases. The formula that describes this relationship is
y = –0.008x + 20.9, where y = corrected oxygen concentration and x = measured relative humidity.
Relative humidity (%) 0 25 50 75 100
Oxygen (%) 20.9 20.7 20.5 20.3 20.1
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