Verfilco Transferpette-12, Transferpette-8 Operating Manual

Liquid Handling · Easy Handling!

Transferpette

®

-8/-12

electronic

Gebrauchsanleitung

F I R S T C L A S S · B R A N D

Operating Manual

Mode d'emploi

Instrucciones de manejo

Istruzioni per l'uso

3

39

75

111

147

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English

Table of Contents

Page

Safety Instructions 40

Functions and Limitations of Use 41

Operating Exclusions 41

Operating Elements 42

The First Steps 43

Setting the Volume 44

Setting the Aspiration and Discharging Speed 45

Correct Pipetting 46

The Pipetting Programs 47

PIP mode 48

PIPmix mode 50

revPIP mode 52

Electrophoresis (GEL) mode 54

DISP mode 56

Checking the Volume 58

Accuracy Table 59

Easy Calibration (adjustment) 60

Autoclaving 62

Reference Run (rEF) 62

Servicing and Cleaning 63

Charging and Replacing the Battery 68

Battery Regeneration Function 69

Troubleshooting 70

Ordering Information · Accessories · Spare Parts 71

Repairs · Contact addresses 72

Calibration Service · Warranty Information 73

Disposal 74

39

Please read the following carefully!

This instrument may sometimes be used with hazardous materials, operations, and equipment.
It is beyond the scope of this manual to address all of the potential safety problems associated with its
use in such applications. It is the responsibility of the user of this pipette to consult and establish appro­priate safety and health practices and determine the applicability of regulatory limitations prior to use.

Safety Instructions

1. Every user must read and understand this

operating manual prior to using the instrument
and observe these instructions during use.

2. Follow general instructions for hazard preven­tion and safety instructions; e.g., wear protec­tive clothing, eye protection and gloves.

When working with infectious or other hazard-

ous samples, all appropriate regulations and
precautions must be followed.

3. Observe all specifications provided by reagent
manufacturers.

4. Never use the instrument in an atmosphere
with a danger of explosion. Highly flammable
liquids must not be pipetted.

5. Only use the instrument for pipetting liquids
that conform to the specifications defined in
the operating exclusions and limitations
(see page 41). If in doubt, contact the manu­facturer or supplier.

6. Always use the instrument in such a way that
neither the user nor any other person is
endangered. Avoid splashes and only use suit­able vessel.

7. Avoid touching the tip orifices when working
with hazardous samples.

8. Never use force on the instrument!

9. Only use original spare parts. Do not attempt

to make any technical alterations. Do not
dismantle the instrument any further than is
described in the operating manual!

10. Before use check the instrument for visible
damages. If there is a sign of a potential
malfunction (e.g., piston difficult to move,
leakage), immediately stop pipetting. Consult
the 'Troubleshooting' section of this manual
(see page 70), and contact the manufacturer
if needed.

11. The original battery must not be replaced with
non rechargeable batteries or rechargeable
batteries of other manufacturers.

12. To charge the NiMH battery pack, use only the
original AC adapter.

13. The AC adapter has to be protected against
moisture and must be used only for this instru­ment.

14. Dispose of batteries only when discharged and
according to applicable regulations.

Improper use of the instrument or the batteries (short circuit, mechanical damage, overheating, incorrect
AC adapter, etc.) can lead to battery explosion.

!

Warning!

40

English

Functions and Limitations of Use

The Transferpette®-8/-12 electronic is a micropro­cessor-controlled, battery-operated piston-stroke
multichannel pipette which uses the air-displace­ment principle for the pipetting of aqueous solu­tions with an average density and viscosity.

When the instrument is used properly, the sample
only comes into contact with the tips and not with
the Transferpette

®

-8/-12 electronic.

41

The Transferpette

®

electronic is intended for the

pipetting of liquids within the following limitations:

– Temperature of both the instrument and solution

should be between 15 °C to 40 °C (59 °F to
104 °F). Consult the manufacturer for use in
temperatures outside of this range.

– Vapor pressure up to 500 mbar

– Viscosity: 260 mPa s (260 cps)

Battery

Nickel-metal hydride battery with 3 cylindrical
individual cells with size AAA, 3.6 V, 700 mAh

AC adapter

Output voltage 6.5 V DC, 200 mA

Limitations of use

Operating exclusions Battery and AC adapter specifications

Viscous and highly adhesive liquids may impair
volumetric accuracy. Volumetric accuracy may also
be impaired when pipetting liquids that differ from
ambient temperature by more than ± 5 °C / 41 °F.

Operating Limitations

The user has to ensure the compatibility of the
instrument with the intended application.

Never use the instrument for pipetting liquids,
that react adversely with polypropylene (PP: shaft
and tips), polycarbonate/polybutyleneterephthalate
(PC/PBT: casing) or EPDM (flexible replacement
pipette shafts). Avoid reactive vapors due to the
danger of corrosion.

The handle is not autoclavable.

Display

Program keys

Tip ejection key

Handgrip

Manifold

Nose cone

Pipetting key

Charging jack

Operating Elements

Menu
selection
key

Entry confir­mation/
Power 'On'*

Pipetting key

Up key (+)

Down key (-)

The Transferpette®-8/-12 electronic is a microprocessor-controlled, battery-

operated, piston-stroke multichannel pipette, which has been optimized for
ergonomic operation and ease of use.

Battery compartment

Finger rest

*) The instrument is activated by pressing the ENTER

key. After pressing the pipetting key next, the
instrument is ready for pipetting.

The Transferpette

®

-8/-12 electronic shuts off 10 min

after the last instrument operation (Auto-Power-Off).

Key Functions

42

The Transferpette®-8/-12

electronic rests easily and

ergonomically in your hand.

The finger rest is height adjust-

able with a screw, which allows

fine-tuning of the hand position

for effortless operation of the func-

tion buttons.

1x

C

B

A

English

c) Replace the battery compartment.

1. Insert the

battery

Is everything in the package?

Confirm that your package includes: Transferpette

®

-8 or -12 electronic
pipette, battery, power supply unit with battery charging cable,
1 TipBox filled, refill unit, 1 instrument stand, 1 reagent
reservoir, silicone oil, operating manual and 1 set of V-rings made of FKM.

a) Open the cover of the battery compartment.

b) Insure that the plug for the battery is firmly
connected to the pipette. Insert the battery.

2. Activate the

instrument

The Transferpette

®

-8/-12
electronic automatically
requests a reference run
directly after the battery is
inserted. After the pipetting
key is pressed, the refer­ence run is carried out and
the instrument is now ready
for pipetting.

Battery charge
indicator

Up arrow,
indicating
aspiration

Pipetting mode

Volume setting

The display shows the
standard factory setting
(pipetting mode/PIP); and
the nominal volume (for
example, 100.0 µl). Default
aspiration and discharging
speeds are at maximum.
The adjustment of volume
and speed is described on
the following pages.

The First Steps

Initializing the Transferpette®-8/-12 electronic

43

1x

+

–

Press the up arrow key (+)
to increase the volume.
Holding the arrow key down
accellerates the rate of
change. ‚VOL‘ continues to
blink.

Press the ENTER key.
The display now shows the
new volume setting, in this

case, 102.8 µl in the PIP
mode.

2. Change the volume

3. Confirm volume

setting

The volume for the Transferpette

®

-8/-12 electronic is set at the factory to the nominal

volume of the instrument and can be changed quickly and easily.

Press the down arrow key
(-) to reduce the volume.
Holding the arrow key down
accellerates the rate of
change. ‚VOL‘ continues to
blink.

Reduce volume

Press one of the arrow keys
to activate volume selection.

‚VOL‘ blinks.

1. Activate volume

setting

Increase volume

Setting the Volume

What to do How to do it Keys to press Display readout

By pressing the MENU key any procedure can be cancelled! The display then moves to the next setting or
back to the initial display (depending on actual selection.)

Important:

44

1x

1x

2x

1x

+

–

+

–

English

Press the MENU key once
to bring up the aspira­tion speed menu. ‚Speed‘
blinks.

Press one of the arrow keys
(+/-) to select the desired
speed (in this case, level 5).
‚Speed‘ continues to blink.

Press the ENTER key. The
display returns to the start
position for the current pipet­ting mode (in this case, the
standard PIP mode).

1. Bring up the menu

2. Change the

aspiration speed

3. Confirm speed

level

The aspiration and discharging speeds can be individually adjusted. When the menu is called up, the last
speed setting is shown. Five speed levels are available.

Press the MENU key twice
to bring up the discharg­ing speed menu. ‚Speed‘
blinks.

Press one of the arrow keys
(+/-) to select the desired
speed (in this case, level 2).
‚Speed‘ continues to blink.

Press the ENTER key. The
display returns to the start
position for the current pipet­ting mode (in this case, the
standard PIP mode).

1. Bring up the menu

2. Change the

discharging speed

3. Confirm speed

level

Setting the aspiration speed

Setting the discharging speed

Setting the Aspiration and Discharging Speed

What to do How to do it Keys to press Display readout

45

1x

1x

Correct Pipetting

with the Transferpette®-8/-12 electronic

1. Attach the tips

Hold the pipette vertically and immerse the tips
2 to 3 mm into the liquid.

Press the pipetting key to
aspirate the liquid into the
tips. The arrow in the display
points upwards to indicate
the aspiration of liquid.

3. Aspirate liquid

Hold the pipette at an angle between 30º and 45º,
place the tips against the vessel wall.

Press the pipetting key again
and the liquid is completely
discharged including auto­matic blowout. Afterwards
wipe pipette tips against the
vessel wall.

4. Discharge liquid

After the liquid has been aspirated, the arrow in the
display points downwards to indicate discharging.

5. Eject tips

Hold the manifold over a
suitable disposal container
and press the tip ejection
key.

Tip ejection
key

The volume is set at the factory to the nominal volume for the Transferpette®-8/-12 electronic

and can be changed quickly and easily. See page 44.

Quick start in the standard pipetting mode

Use the correct tips according to the volume range or the color code. Ensure
that the tips are securely seated.

Pipette tips are disposables items!

To avoid the intake of air, leave the tips immersed
into the liquid for approx. 1 sec.

Note:

46

The manifold can turn freely in both directions.

2. Align the manifold

Note:

ISO 8655 prescribes rinsing the pipette tips once with the sample liquid prior
to the actual pipetting process.

English

The Pipetting Programs

1. Normal Pipetting

PIP Mode

Standard program. A previously set volume
is aspirated into the pipette tip and then
discharged.

48

4. Pipetting for Electrophoresis

GEL Mode

Program for loading electrophoresis gels. A
predefined sample volume is aspirated at high,
adjustable speed and then slowly discharged.

54

2. Mixing of Samples

PIPmix Mode

Program for mixing liquids. The sample is
repeatedly aspirated and discharged.

50

3. Reverse Pipetting

revPIP Mode

Program especially for pipetting liquids with a
high viscosity or vapor pressure, or that tend
to foam.

52

5. Dispensing

DISP Mode

Program for dispensing liquids. An aspirated
volume is dispensed repeatedly in defined
steps.

56

47

Page

3x

1x

1x

1x

➞

Press the MENU key three
times to bring up the mode
selection menu. ‚Mode‘
blinks.

Use one of the arrow keys
to scroll through the modes
until ‚PIP‘ appears.
‚Mode‘ continues to blink.

Press the ENTER key.
The display now shows ‚blo‘
for blow-out.

Press the pipetting key once
to aspirate the liquid.

Press the pipetting key once
to move the pistons into the
start position.
The arrow in the display
points upwards (aspiration).

PIP Mode

The Programs · Settings and Use

The standard program – a previously set volume is aspirated and then discharged.
Volume and speed adjustments are described on pages 44 and 45.

1. Bring up the menu

2. Select PIP mode

3. Confirm PIP mode

4. Prepare for

pipetting

5. Aspirate liquid

What to do How to do it Keys to press Display readout

48

1x

1x

➞

1x

English

Press the pipetting key once
to discharge the liquid. The
arrow in the display points
downwards (discharge).

No action required!

When pipetting in the PIP
mode the blow-out function

is performed automati­cally.

6. Discharge liquid

7. Start blow-out?

Press the ENTER key. The
display shows ‚blo‘
for blow-out.

1. Bring up the

blow-out function

Press the pipetting key
once to initiate the blow-out
process. The display moves
back to the start position of
the selected pipetting mode.

2. Start blow-out

Start blow-out manually

The blow-out function can, if necessary, be initiated manually
at any time.

PIP Mode

What to do How to do it Keys to press Display readout

To accomplish the blow-out, the pistons move to the lowest position.
The user must be certain that any residual liquid is discharged safely.

If the pipetting key is pressed and held, the pistons will be maintained
at their lowest position to avert an accidental aspiration of liquid.
When the key is released, the pistons return to the start position

Note:

49