Varian ProStar 701 Driver Manual

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Varian, Inc. 2700 Mitchell Drive Walnut Creek, CA 94598-1675/USA
ProStar 701 Fraction
Collector Control
©Varian, Inc. 2005 03-914947-27:Rev.2
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Table of Contents
Introduction.............................................................................................. 2
General................................................................................................................................2
Installation and Configuration................................................................ 3
Installing the Driver Software...............................................................................................3
Configuring the Communication Server...............................................................................3
Configuring the Driver..........................................................................................................4
Configuring the Varian ProStar 701 Fraction Collector.................................................4
Configuring the Synopsis..............................................................................................5
Building a Method ................................................................................... 7
Creating a New Method.......................................................................................................7
Control Method Section for the Varian ProStar 701 Fraction Collector.............................10
Collector Parameters Control......................................................................................12
Fraction Collector Mode Control.................................................................................14
Rack Control ...............................................................................................................17
Collection Chain Control .............................................................................................19
Starting and Viewing an Acquisition.................................................... 21
Quick Start and Sequence.................................................................................................21
Quick Start (Single Acquisition) ..................................................................................21
Sequence....................................................................................................................23
Running Acquisition Status................................................................................................24
Viewing a Running Acquisition....................................................................................24
Status of the Varian ProStar 701 Fraction Collector...................................................24
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Introduction

General

This document describes the configuration of the Varian ProStar 701 Fraction Collector driver. It also explains how to build a control method for this driver, how to start and view a running acquisition and what actions are available during an analysis.
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Installation and Configuration

Installing the Driver Software

To install the Galaxie Drivers software refer to the Galaxie Data System Installation Guide.

Configuring the Communication Server

To communicate with the ProStar 701 module an RS232_Interface or an RS232_PC communication bus must be configured on the acquisition server.
The parameters of the bus to must be the following:
Stops 1 Stop
Baud 19200 Parity EVEN Bits 8 Bits Flow Control NONE Buffer Tx 4096 Buffer Rx 4096
NOTE: For more details about the configuration of an RS232_Interface or an RS232_PC
bus, refer to the Galaxie Chromatography Data System Installation’s Guide.
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Configuring the Driver

The driver software configuration is done in the Galaxie Configuration Manager when a new chromatographic system is created.

Configuring the Varian ProStar 701 Fraction Collector

Click on the button corresponding to the Varian ProStar 701 Fraction Collector driver and the configuration screen appears.
Communication Window
Select from the Hardware Interface list the name of the RS232 Bus where the system has been connected.
NOTE: For more details about the RS232 Bus configuration, refer to the
Configuring the Communication Server section in this manual.
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Define the number of ProStar 701 Fraction Collector installed in the Number of instrument spin edit. Up to four ProStar 701 Fraction Collectors can be defined.
For each fraction collector, define the address in the Fraction Collector X ID field. Then press the
button, to connect to the instrument.

Configuring the Synopsis

In the Overview part of the system configuration, a synopsis can be defined for the system. This synopsis depends on the configured devices.
1. To save the configuration of these devices, click on the OK button.
2. Right click on the system name in the bottom part of the Galaxie Configuration Manager screen. A pop-up menu appears: click on Stop. Then right click on the system name again, and click on Start.
3. Go back to the System Configuration screen (Properties wizard). A graphic of the devices that comprise the system is displayed in the synopsis screen. To configure the synopsis as the real instrument configuration, move each module, and then connect it appropriately. To do this, select one anchor for each of the two modules (the selected
anchors become red) and click on the connection tube will appear between the two anchors.
Click on the button to delete all the tubes.
Click on make the background transparent.
Click on the
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to delete a selected tube, click on the
to define a background color, or on to
icon to change the grid color.
button; a
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4. Once the synopsis configuration is complete, it will be displayed in the Galaxie Chromatography Data System, in the Systems tab. In this tab, the Overview part will show the synopsis of the system with some parameters displayed (temperature, flow, signal, etc.) depending on the installed devices.
For example, the following pictures represent the synopsis of the Varian ProStar 701 Fraction Collector:
Galaxie Configuration Manager Screen
Galaxie Chromatography Data System Screen
NOTE: Once the system has been created and correctly configured, it must be
associated with one or more projects in order to start some acquisitions. Fo r more details about the creation and configuration of a system, refer to the Galaxie Configuration Manager User’s Guide.
Click on the
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button to print the current configuration.
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Building a Method

Creating a New Method

The method contains parameters for instrument control, data acquisition, chromatogram processing, and editing of final results. To initially set up a method, it is not necessary to define all of the sub-method sections; however, you must fill in the instrument control section prior to starting an acquisition.
1. To create a new method, select the FILE / NEW METHOD option from the Galaxie Chromatography Data System main menu.
A wizard guide will appear and provide assistance during the first steps of method creation:
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2. Choose the system (chromatograph) associated with the method. A method is created for a particular system. When starting an acquisition, the name of the system that performs the acquisition must be correct, since method access is limited only to those methods associated with this system.
3. Once this field is completed, click on the Next button to move to the second step of the method creation:
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4. Enter the New Method Name in the first field. In the Description field, enter information concerning the method
(optional).
5. Once these two fields have been completed, click on the OK button. The new method is created and is opened with default parameters in the Galaxie Chromatography Data System. The next step is the definition of each of the method sections.
The instrument control section must be defined in order to start an acquisition. In the following part of this guide, only the control section for the Varian ProStar 701 Fraction Collector is detailed. Other parameters such as Injection Volume, Vial and Rack number may be necessary and are defined in the acquisition section.
NOTE: For more details about the programming of each method section, turn to the
Galaxie Chromatography Data System User’s Guide.
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Varian ProStar 701 Fraction Collector Control Method

Once the new method is created, select the control section in the lower part of the browser.
The following screen appears:
Press the Advanced Tools window:
to send the current method to the instrument.
to get the active method from the instrument.
to print the current method.
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button to display the System Control Method
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Click on the
button to display the ProStar 701 Fraction
Collector control method section. It contains four sub-sections:
Collector Parameters, Fraction Collector Mode, Racks, and Collection Chain. Click on the required sub-section to define the
corresponding parameters that will be used during the chromatographic run. The toolbar allows you to easily scroll through the control method windows:
to select the first window of the control method.
to move back to the previous window of the control
method.
to move forward to the next window of the control
method.
to select the final window of the control method.
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Collector Parameters Control

Collector Parameters Parameter Group:
Select from the Mode list the way the fraction size is defined:
None: no collection Time: fraction size based on time Drop: fraction collection by drop
Note that some parameters of the other sub-sections will be activated or may change according to the entry.
Select in the Pattern list weather the fraction collector uses the serpentine or the unidirectional mode to collect samples.
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Serpentine Unidirectional
Define in the Flow delay field the time delay (in seconds) between when a peak is sensed and when the collection valve is activated. This option is available only if Slope or Level options are checked in the Fraction Collection Mode sub­section. The values run from 0 to 3559 sec.
Define in the Collection Option list where the peaks and non­peaks are sent:
Peaks =Rack1&2; Non-peaks= Valve Peaks =Rack1&2; Non-peaks= Drain Peak/Non-peaks=Consecutive tubes Uploaded.
The Uploaded entry is the entry that is entered into the front panel of the fraction collector. This allows the use of collection options that cannot be sent to the fraction collector. If this is selected, the collection option in the fraction collector will be used.
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Fraction Collector Mode Control

Fraction Collector Mode Parameter Group:
In the Sense Peak By options, define the way the presence of peaks will be determined:
Time: peaks are determined by time. The Fraction Windows table is activated. The user has to determine
the time size of each fraction. Slope: peaks are sensed by their rate of change of
signal. As a peak starts rising, it will be sensed and collection will be started. The peak separation is based on the range in the slope of the baseline over a wide range of user defined peak widths. If selecting this
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option, complete the three fields Slope sensitivity, Peak Width and Detector Signal.
Level: peaks are sensed by their absolute level above
baseline. This option does not involve the collection of the entire peak, but only of the part that rises above the threshold level (defined in the Threshold Level field). This mode of collection allows collecting only the most concentrated portion of the peak. Collection of peaks is based on percentage of full scale height. If 0% is defined in the Threshold level field, the Level feature is canceled. If selecting this option, complete the two fields Detector Signal and Threshold Level.
User can combine Slope or Level collection modes with Time by using logical AND Time or OR Time. If AND Time is selected, the peak will only be collected when both a time window is active, and the slope or level detection conditions are met. If OR Time is selected, the peak will be collected if either the time condition or the slope or level conditions are met.
If Slope and Level are selected together, they are always linked with the AND logical operator.
Slope sensitivity: define the range of sensitivity, two choices are available: Normal and High. The Normal sensitivity requires a larger slope change before indicating a peak. The High sensitivity should be used with smooth baselines to collect the maximum sample of broad based peaks or smaller peaks.
Peak Width: it is the expected average peak width at baseline. Values are proposed in the scrolling list.
Detector Signal: it is the detector output value. Three choices are available: 10mV, 100mV and 1 V.
Threshold level: it is the percentage (0 to 100%) of the full scale height since which peaks are collected.
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Fraction Windows: this table has to be filled if the Time mode of
collection is activated. User has to define the fraction time sizes. Up to 25 lines can be defined.
Use the following buttons to edit the table:
to add a line to the table.
to delete a line in the table.
to clear the entire table.
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Rack Control

Rack X Parameter Group:
Define the rack(s) parameters. Two racks can be defined. According to the chosen rack in the RackSize list, the Tubes/Rack field is automatically filled in.
The value displayed by default in the Tubes/Rack field is the maximum tubes available in the rack; this value can be reduced by the user.
In the Fraction Size field enter the maximum number of seconds or drops (depending on whether Time or Drop mode is defined in the Collector Parameters screen) that can be contained in a tube. If this value is exceeded, the fraction collector moves to
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another tube. The peak is then divided into several fractions (1 fraction =1 tube).
Rack size Max tube number Comments
No rack
12/13 mm tubes
16 mm tubes
18 mm tubes
17 mm vials
25/28 mm vials
Microvials
Prep funnel
Bottles
Beaker
Custom
0
144
100
72
72
36
60
26 not available for Rack 2
9
1
Use of a rectangular rack with max 16 rows and 16 tubes per row. User has to define the first and last tube position, ProStar 701
256
Fraction Collector will calculate the row and column spacing. The configuration is done from the ProStar 701 Fraction Collector instrument.
Allow user to program up to 50 tube
Random
50
positions per rack in any sequence and position. The configuration is done from the ProStar 701 Fraction Collector instrument.
Microplate
192
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Collection Chain Control

Collection Chain Parameter Group:
This sub-section allows the user to overlay collections in same tubes. For example to concentrate peaks, user can inject several similar samples via a sequence of injections, and collect fractions in same tubes: all the fractions 1 of the X samples are sent in the same tube, all the fractions 2 of the X samples are sent in the same tube…(where X is the Overlay Number).
In the Overlay Number field, define the number of time the collector drops solution in the tube. If 3 is defined, that means that three successive injections will be distributed in the same tubes, because the same fractions are defined for the three injected samples. To use the Collection Chain option, it is
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advised to use the Sense Peak by Time option in the Fraction Collector Mode sub-section.
Note that the overflowing is not managed by the ProStar 701 Fraction Collector in the Overlay case which means the Fraction Size parameter (Racks sub-section) is not taken into account.
In the Sample Spacing field, define the way two consecutive injection collections are separated:
Next tube: consecutive injections are put in consecutive tubes (the last fraction of the first injection and the first fraction of the second injection are dropped in consecutive tubes).
Next Row: the collector return to a new row between two consecutive injections.
Skip One Tube: consecutive injections are separated by one empty tube
Advance to next available collector at run end, if remaining clean tubes number is less than: This option is available only if
several fraction collectors have been configured. If the number of clean tubes is less than the specified one, the collection is switched to the following fraction collector.
Abort run, if remaining clean tubes number less than: If the number of clean tubes is less than the specified one, the run is aborted.
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Starting and Viewing an Acquisition

Quick Start and Sequence

Once the instrument control method has been correctly programmed, an analysis can be started. The next section briefly describes how to start an acquisition.

Quick Start (Single Acquisition)

1. Press the button.
In the Quick Start window, select the system, the method and optionally the project if connected in "All project" mode.
2. Press the OK button.
The following screen is displayed:
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In the Acquisition parameters tab, required fields like the Run Name, the Run ID and the Acquisition length must be
filled in before starting an analysis. Furthermore, if the system is equipped with an autosampler controlled by the Galaxie Chromatography Data System, the Injection Volume, the Vial position and the Rack number (if required) must be entered.
Other parameters associated with each acquisition channel can be entered in the Channel X tab.
3. Click on the Start button to start the acquisition if the system is a single-injector system or click on the Next button if the system is a multiple injector system. In a multiple injector system, the same parameters entered for the first injector must be entered for the other injectors before starting the acquisition.
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Sequence

1. To create a sequence, select the FILE / NEW SEQUENCE menu, and complete the displayed creation wizard screen (refer to the Galaxie Chromatography Data System User’s Guide), by entering the system name that will perform the analysis, the number of lines (corresponding to the number of samples), and the sequence name.
2. The sequence is displayed. Complete the sequence information columns (refer to the Galaxie Chromatography Data System User’s Guide).
The Method Name, the Run Name, the Run ID, and the Acquisition length must be filled in before starting the analysis. All these parameters are automatically imported from the acquisition part if defined in the method.
3. Start the sequence by clicking on the
NOTE: For more details about how to start an acquisition, refer to the Galaxie
Chromatography Data System User’s Guide.
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button.
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Running Acquisition Status

Viewing a Running Acquisition

To view a running acquisition, click on the Systems tab then select the running system. A window is activated which is composed of two parts: the running chromatogram and the status window where the driver modules are listed.

Varian ProStar 701 Fraction Collector Status

Click on the button corresponding to the ProStar 701 Fraction Collector driver and the fraction collector status screen will appear.
Click on the
to display the Overview screen.
Status Window
Instrument Status Parameter Group:
Gives the state of the instrument, specifically whether it is locally controlled (Local State) or under Galaxie control (Remote State), and gives details about used racks.
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Current Position Parameter Group:
Displays the position of the fraction collector (collector number, rack number and tube number).
Commands Parameter Group:
If no acquisition is performing, all the following buttons are available:
: allows the user to change the collector position. When clicking on this button, the following screen appears:
The user defines the rack and the tube positions where the collector has to move to. If selecting the
button the
collector moves to its initial position.
: moves to the following tube.
: moves to the next rack and keeps the same tube number
position.
: allows to clear the synopsis screen (displayed in the Overview
tab). The user has to activate this button between two acquisitions to reset the rack (if he replaces tubes containing fractions by empty ones); if not, the fractions of the second acquisition will be put at the end of the first acquisition ones.
: activating this button displays the following screen:
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The user has to select the program stored in the ProStar 701 Fraction Collector driver to start.
: when pressing this button a message asks to confirm the action
and the button is transformed into
. This forces the ProStar 701 Fraction Collector to send the solution in the Drain (waste) or to collect it in tubes.
: this allows the user to work in local or to take the control of the
ProStar 701 Fraction Collector via Galaxie (Remote).
If an acquisition is performing, the following buttons are available:
: allows the user to change the collector position. When clicking on
this button, the following screen appears:
The user defines the rack and the tube positions where the collector has to move to. If selecting the
button the
collector moves to its initial position.
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: moves to the following tube.
: moves to the next rack and keeps the same tube number position.
: allows the user to clear the synopsis screen (displayed in the
Overview tab). User has to activate this button between two acquisitions to reset the rack (if he replaces tubes containing fractions by empty ones); if not, the fractions of the second acquisition will be put at the end of the first acquisition ones.
: activating this button stops the fraction collection. User can stop
and start again the collection during an acquisition, the log will be created according these stop/start actions. Note that when performing collection under Galaxie control, the program A of the ProStar 701 Fraction Collector is erased and replaced by the one defined in the Galaxie method.
Overview Window
Click on the button: the Status Overview window is displayed and contains three tabs:
General: displays the synopsis of the instrument, where
some current values can be viewed: rack filling in, racks properties.
If moving the cursor into a rack, the tube position is mentioned in the left bottom corner of the first rack.
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The buttons available in the right part of the Overview part have the same functions as in the Status window.
Not Ready: no information is listed.  Events: contains the communication state between
Galaxie and the ProStar 701 Fraction Collector.
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