Varian 900-LC Series Getting Started Manual

Varian 900-LC Series
Liquid Chromatograph

Getting Started Manual

Installation Category II
Pollution Degree 2
Safety Class 1 (EN 61010-1)

8510243600
December 2007

Varian 900-LC Series Liquid Chromatograph

Varian, Inc. offices

Varian, Inc. has offices in most countries. The major offices for liquid
chromatography products are listed below:

Varian Australia Pty Ltd (Manufacturing site)
679 Springvale Road
Mulgrave, Victoria 3170
Australia
International telephone: + 61 3 9560 7133
International fax: + 61 3 9560 7950

Varian Instruments
2700 Mitchell Dr
Walnut Creek, CA 94598
Telephone: [phone]
International telephone: [phone]
International fax: [phone]

Varian BV
Herculesweg 8
4338 PL, Middelburg, Netherlands
International telephone: [phone]
International fax: [phone]

Internet

The Varian, Inc. Internet home page can be found at:
www.varianinc.com

Varian Australia Pty Ltd is the owner of copyright on this document
and any associated software. Under law, the written permission of
Varian Australia Pty Ltd must be obtained before either the
documentation or the software is copied, reproduced, translated or
converted to electronic or other machine-readable form, in whole, or
in part.

First published December 2007 in Australia. Comments about this
manual should be directed to the Marketing Communications
Manager, Varian Australia at the address above or by e-mail to:
[e-mail]

Varian Australia is ISO9001:2000 certified.

©2007 Varian Australia Pty Ltd (ABN 67 004 559 540)
Galaxie, Varian and the Varian logo are trademarks or registered
trademarks of Varian, in the U.S. and other countries.

All rights reserved.

2 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

Declaration of Conformity

We hereby declare that the equipment listed below complies with the requirements of:

The Low Voltage Directive 73/23/EEC (93/68/EEC)

The EMC Directive 89/336/EEC (92/31/EEC and 93/68/EEC)

Applicable Standards
LVD BS EN 61010-1:1993
EMC BS EN 61326:2003 EN 55011:1999 EN 61000-4-2:1995
EN 61000-4-3:2003 EN 61000-4-4:1995 EN 61000-4-5:1995
EN 61000-4-6:1996

Equipment Model Number Varian Model Instrument Type

Authorized Representative in the EU

Name: G. A. Wassink Company Name Varian BV

Address Herculesweg 8

4330 EA Middelburg

Signed:

The Netherlands
Position: Quality Manager Telephone [phone]
Date: 1 Month 20XX Facsimile [phone]

Manufacturer

Name: Phil Binns Company Name Varian Australia Pty Ltd

Address 679 Springvale Road

Mulgrave VIC 3170
Signed:

Australia
Position: Director of Operations Telephone [phone]

Date: 1 Month 20XX Facsimile [phone]

Publication number 8510243600 Month 20XX

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Varian 900-LC Series Liquid Chromatograph

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Varian 900-LC Series Liquid Chromatograph

Contents

Safety Practices and Hazards................................................................9

General 9
Electrical Hazards 10
Compressed Gas Cylinders 11
High Pressure Hazards 12
Ultraviolet Radiation 13
Flash Chromatography 13
Solvent Hazards 13

Ignition of Flammable Chemicals 14

Inflammation or Injury due to Toxic, Corrosive or Stimulative

Solvent 14

Explosion of Vapor from Flammable Chemicals 14
Other Precautions 15
Warning and Caution Messages 15
Warning Symbols 16
Color Coding 17
US FCC Advisory Statement 17
Spurious and Harmonic Radiation Compliance 18
CE Compliance 18

1. Introduction ................................................................................ 19

1.1 Pre-installation Requirements 19

1.2 User Documentation 19

1.2.1 Conventions 19

1.2.2 Notes and Hot Tips 20

1.3 Specifications 20

1.3.1 Environmental Conditions 20

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Varian 900-LC Series Liquid Chromatograph

1.3.2 Electrical Power Supplies 21

1.3.3 Gas Supplies 23

1.3.4 Waste Container 24

1.3.5 Laboratory Conditions 24

1.3.6 Instrument Cooling Air Supply 24

1.3.7 Weights and Dimensions 25

1.4 Personal Computer Requirements 26

2. Installation .................................................................................. 29

2.1 900-LC Series LC System to PC Communications 29

2.2 Installing the Galaxie™ Chromatography Data System 32

2.3 About the Varian Integrated HPLC Online Help 40

2.4 Hardware Installation 41

2.5 Installation Qualification Tests 41

2.6 Customer Training 41

2.7 Using the Online Help 42

2.8 Instrument Maintenance Software (IMS) 42

2.8.1 How to launch IMS 42

2.8.2 About IMS 43

3. Operation..................................................................................... 45

3.1 Analysis Checklist 45

3.2 Turning on the Instrument and Software 45

3.2.1 Connecting to a System 46

3.3 Preparing the System for Use 46

3.4 Configuring the Device Settings and System Parameters 47

3.4.1 Flows 48

3.4.2 Detectors 48

3.4.3 Column 48

3.4.4 Sample Introduction 49

3.4.5 System Parameters 49

3.5 Creating a Method 50

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Varian 900-LC Series Liquid Chromatograph

Starting an Acquisition 52

3.6

3.6.1 Sequence 52

3.6.2 Quick Start 52

3.7 Printing a report 53

3.8 Saving Your Files 54

4. Maintenance................................................................................ 55

4.1 Routine Maintenance 56

4.2 Cleaning 57

4.3 Spare Parts 57

5. Troubleshooting .......................................................................... 59

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Varian 900-LC Series Liquid Chromatograph

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Varian 900-LC Series Liquid Chromatograph

Safety Practices and Hazards

Your Varian instrument and accessories have been carefully
designed so that when used properly you have an accurate, fast,
flexible and safe LC system.

Information about safety practices appears throughout the
documentation (both hard copy and online) provided with your
instrument. Before using the instrument, you must thoroughly read
these safety practices.

Observe all relevant safety practices at all times.

This instrument is designed for chromatographic analysis of
appropriately prepared samples. It must be operated using
appropriate solvents and within specified maximum ranges for
pressure, flows, and temperatures as described in this manual. If
the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.

It is your responsibility to inform Varian representatives if the
instrument has been used for the analysis of hazardous biological,
radioactive, or toxic samples, prior to any instrument service being
performed or when an instrument is being returned to the service
center for repair, or is being recycled at end-of-life.

General

Operation of a Varian 900-LC Series Liquid Chromatograph
involves the use of pressurized liquid, ultraviolet radiation, and
hazardous materials including corrosive fluids and flammable
liquids. Careless, improper or unskilled use of this liquid
chromatograph can cause death or serious injury to personnel,
and/or severe damage to equipment and property.

The 900-LC Series Liquid Chromatograph incorporates covers that
are designed to prevent inadvertent contact with any potential
hazards. If the instrument is used in any manner not specified by

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Varian 900-LC Series Liquid Chromatograph

Varian, this protection may be impaired. It is essential that no
cover is bypassed, damaged or removed.

The safety practices described below are provided to help the user
operate the instrument safely. Read each safety topic thoroughly
before attempting to operate the instrument and ALWAYS operate
the liquid chromatograph in accordance with these safety
practices.

Follow these safety practices to ensure safe equipment operation.

• Perform periodic leak checks on all supply lines and
pneumatic plumbing.

• Do not allow liquid lines to become kinked or punctured. Place
lines away from foot traffic and extreme heat or cold.

• Store organic solvents in fireproof, vented and clearly labeled
cabinets so they are easily identified as toxic and/or
flammable materials.

• Keep the waste vessel in view and empty as required.

• Do not accumulate waste solvents. Dispose of such materials

through a regulated disposal program and not through
municipal sewage lines.

This instrument has been tested per applicable requirements of
EMC Directive as required to carry the European Union CE Mark.
This equipment may be susceptible to radiation/interference levels
or frequencies, which are not within the tested limits.

Electrical Hazards

Exposure to high voltages and UV energy can cause severe skin
damage and cataracts of the eyes, while close contact with the
electrical components can result in severe heat burns to the skin,
and an electrical discharge which may cause death, severe electric
shock or sub-surface skin burns.

The 900-LC Series Liquid Chromatograph has been carefully
designed to operate safely and effectively when using components
that conform to Varian’s design criteria. Use of non-approved
components in the LC system may render the system inoperative
and/or hazardous. It may also invalidate the warranty on the
instrument. Use only related components supplied or authorized by
Varian.

• Disconnect the instrument from all power sources before

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Varian 900-LC Series Liquid Chromatograph

removing protective panels to avoid exposure to potentially
dangerous voltages. Panels or covers which are retained by
screws on the LC system may be opened ONLY by Varian­trained, Varian-qualified, or Varian-approved Representatives.
Consult the manuals or product labels supplied with your PC
to determine which parts are operator-accessible.

• When it is necessary to use a non-original power cord plug,
make sure the replacement cord adheres to the color coding
and polarity described in the manual and all local building
safety codes.

• Good grounding/earthing is essential to avoid a potentially
serious electric shock hazard. Consult the manuals or product
labels supplied with your PC for the relevant grounding
requirements.

• Replace blown fuses with fuses of the size and rating shown
on the fuse panel or in the manual.

• Replace faulty or frayed power cords immediately with the
same type and rating.

• Make sure that voltage sources and line voltage match the
value for which the instrument is wired.

• Avoid using power supplies from a source that may be subject
to electrical or RF interface from other services (for example,
large electrical motors, elevators and welders).

Compressed Gas Cylinders

Unless your system is equipped with a helium sparging unit or an
ELS detector, there are no gas requirements for the 900-LC Series
LC systems. For gas requirements on these devices, consult the
Operation Manual or other documentation enclosed with the
particular device. Compressed gas cylinders contain highly
pressurized gas. If storage conditions are outside of the
recommended suppliers safety codes the cylinders can explode or
rapidly release gas into the environment. This may result in injury
or death.

• Store and handle compressed gases carefully and in strict
adherence to safety codes.

• Secure cylinders to an immovable structure or wall.

• Store and move cylinders in an upright, vertical position.

Before transport, remove regulators and install cylinder cap.

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Varian 900-LC Series Liquid Chromatograph

• Store cylinders in a well ventilated area away from heat, direct
sunshine, freezing temperatures, and ignition sources.

• Mark cylinders clearly so there is no doubt as to their
contents.

• Use only approved regulators and connections.

• Use only connector tubing that is chromatographically clean

(Varian part number 0391832600) and has a pressure rating
significantly greater than the highest outlet pressure from the
regulator.

Storage Cylinder Instructions

Cylinders containing gas under pressure should be firmly secured
to a rigid structure, and the storage area must be adequately
ventilated.

Never locate gas cylinders near a source of ignition, or in a
position that is subject to direct heat. Gas storage cylinders often
incorporate a pressure release device which will discharge the gas
at a predetermined temperature, usually around 52 °C (125 °F).

If gases are to be plumbed from a remote storage area to the
instrument site, ensure that the local outlets are fitted with stop
valves, pressure gauges and suitable regulators which are easily
accessible to the instrument operator. The gas outlets must be
provided within 1.5 metres (5 feet) of the instrument

High Pressure Hazards

If a line ruptures, a relief device opens, or a valve opens
accidentally under pressure, potentially hazardous high liquid
pressures can be generated by the pump causing a high velocity
stream of volatile and/or toxic liquids.

• Wear personal protective equipment when you inject samples
or perform routine maintenance.

• Never open a solvent line or valve under pressure. Stop the
pump first and let the pressure drop to zero.

• Read and adhere to all NOTES, CAUTIONS, and WARNINGS
in the manual.

• Always keep the doors and covers closed during operation.

12 Publication date: December 2007

Ultraviolet Radiation

900-LC Series Liquid Chromatograph systems that use an
ultraviolet light source have shielding to prevent radiation exposure
to personnel.

For continued protection:

• Ensure that any protective lamp covers are in place during
operation.

• Do not look directly into detector fluid cells or at the UV light
source. When inspecting the light source or fluid cell, always
use protective eye covering such as borosilicate glass or
polystyrene.

• Ozone can be generated by radiation from source lamps.
Exposure to ozone can result in severe irritation to the skin,
eyes, and upper respiratory system. The maximum
permissible exposure level is 0.1 ppm (0.2 mg/m
ventilate the area surrounding the LC system such that the
concentration of ozone does not exceed the maximum
permissible level. All venting must be to outside air, never
within the building.

Varian 900-LC Series Liquid Chromatograph

3

). ALWAYS

Flash Chromatography

The operator should be familiar with the physico-chemical
properties of the components of the mobile phase.

Only use solvents compatible with the LC system tubing and
fittings as certain solvents may cause weakening and leaks with
possible bursting.

Employ static measuring and static discharge devices to safeguard
against the buildup of static electricity.

Solvent Hazards

All solvents can create a hazard if they leak into the atmosphere.
Even small leaks in solvent supply systems can be dangerous. Any
leak (except that of air) can result in an oxygen-deficient
atmosphere, which can cause asphyxiation. The area in which
solvents are stored and the area surrounding the instrument must
be adequately ventilated to prevent such accumulations.

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Varian 900-LC Series Liquid Chromatograph

Ignition of Flammable Chemicals

This instrument is not explosion-proof. In unattended operation, do
not use organic solvents having an ignition point below 70 °C.

Beware of ignition hazard when using flammable chemicals such
as organic solvents.

Do not bring a heat or flame source near the instrument.

Well-ventilate the laboratory room where the instrument is used.

Always check the following conditions. If an abnormality is found
stop operation immediately.

• Leakage of solvent or waste solution.

• Leakage of solvent inside the instrument.

When using flammable chemicals, be careful about possible
ignition due to static electricity. To prevent the build-up of static
electricity, use a conductive container for waste.

Inflammation or Injury due to Toxic, Corrosive or Stimulative Solvent

When using a toxic, corrosive or stimulative solvent, be careful not
to incur a physical inflammation or injury. For details of the
properties of each solvent and how to handle it, refer to the
relevant Material Safety Data Sheets (MSDS). Be sure to handle
each solvent properly.

Wear proper protective clothes (e.g., safety goggles) so that a
solvent will not come into direct contact with the skin.

Ventilate the laboratory room adequately to prevent accidental
inhalation of harmful solvent vapor.

Explosion of Vapor from Flammable Chemicals

If a flammable chemical such as organic solvent leaks from the
flow path of the instrument and its vapor concentration reaches the
explosion limit, it could cause spontaneous combustion with
dangerously explosive results.

When using a flammable and readily volatile chemical, be sure to
check for leakage from the instrument flow path and ventilate the
laboratory room adequately.

Use only approved regulator and hose connectors (refer to the

14 Publication date: December 2007

supplier’s instructions). Keep solvents cool and properly labeled.
Ensure that you have the correct solvent before connecting it to the
instrument.

Other Precautions

Use of the 900-LC Series Liquid Chromatograph and accessories
may involve materials, solvents and solutions, which are
flammable, corrosive, toxic or otherwise hazardous. Careless,
improper or unskilled use of such materials, solvents and solutions
can create explosion hazards, fire hazards, toxicity and other
hazards that can result in death, serious personal injury or damage
to equipment.

Always ensure that laboratory safety practices governing the use,
handling and disposal of hazardous materials are strictly observed.
These safety practices should include wearing appropriate safety
clothing and safety glasses.

Airflow to the cooling fans of the liquid chromatograph must be
unobstructed. Do not block the ventilation grills on the liquid
chromatograph and accessories. Consult the manuals supplied
with your PC, monitor and for their specific ventilation
requirements.

Varian 900-LC Series Liquid Chromatograph

Great care should be taken when working with glass or quartz
parts to prevent breakage and cuts.

The 900-LC Series Liquid Chromatograph weighs approximately
140 kg (309 lb). To avoid injury to personnel or damage to the
instrument or property, always use appropriate lifting procedures to
move the instrument.

Use only Varian-supplied spares with your instrument.

Warning and Caution Messages

A Warning message is used in the text when failure to observe
instructions or precautions could result in death or injury. The list of
symbols that appear in conjunction with warnings are detailed in
the next section.

A Caution message is used when failure to observe instructions
could result in damage to equipment (Varian supplied and/or other
associated equipment).

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Varian 900-LC Series Liquid Chromatograph

Warning Symbols

The following is a list of symbols that appear in conjunction with
warnings in this manual or on the 900-LC Series Liquid
Chromatograph. The hazard they describe is also shown.

A triangular symbol indicates a warning. The meanings of the
symbols that may appear alongside warnings in the documentation
or on the instrument itself are as follows:

Corrosive liquid

Eye hazard

Heavy weight

(danger to hands)

Radiation source

The following symbol may be used on warning labels attached to
the instrument. When you see this symbol, refer to the relevant
operation or service manual for the correct procedure referred to
by that warning label.

Electrical shock

Fire hazard

Hot surface

Explosion hazard

Heavy weight

(danger to feet)

Moving parts

The following symbols appear on the instrument for your
information.

16 Publication date: December 2007

I

Mains power on

Varian 900-LC Series Liquid Chromatograph

Color Coding

The various indicator lights appearing on Varian instruments and
associated accessories are color coded to represent the status of
the instrument or accessory.

• A green light indicates the instrument is in normal/standby

0

mode.

Mains power off

Fuse

Single phase alternating current

Direct current

When attached to the rear of the instrument,
indicates that the product complies with the
requirements of one or more EU directives.

When attached to the rear of the product, indicates
that the product has been certified (evaluated) to
CSA 1010.1 and UL 3101-1.

• An orange light indicates that a potential hazard is present.

• A blue light indicates that operator intervention is required.

• A red light warns of danger or an emergency.

US FCC Advisory Statement

This equipment generates, uses and can radiate radio frequency
energy, and if not installed and operated in accordance with the
instruction manual may cause interference to radio
communications. It has been tested and found to comply with the
limits for a Class A computing device pursuant to Subpart J of Part
15 of Federal Communications Commission (FCC) Rules, which
are designed to provide reasonable protection against such
interference when operated in a commercial environment.
Operation of this equipment in a residential area may cause
interference, in which case the user will be required to take
whatever measures may be necessary to correct the interference
at his or her expense.

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Varian 900-LC Series Liquid Chromatograph

Spurious and Harmonic Radiation Compliance

Your Varian 900-LC Series Liquid Chromatograph complies with
the requirements of FCC rules and Regulations part 18, subpart
H—Miscellaneous Equipment, at the date of manufacture.

CE Compliance

Your Varian 900-LC Series Liquid Chromatograph has been
designed to comply with the requirements of the Electromagnetic
Compatibility (EMC) Directive and the Low Voltage (electrical
safety) Directive (commonly referred to as the LVD) of the
European Union. Varian has confirmed that each product complies
with the relevant Directives by testing a prototype against the
prescribed EN (European Norm) standards.

Proof that a product complies with these directives is indicated by:

• The CE Marking appearing on the rear of the product, and

• The documentation package that accompanies the product

containing a copy of the Declaration of Conformity. The
Declaration of Conformity is the legal declaration by Varian
that the product complies with the directives listed above, and
shows the EN standards to which the product was tested to
demonstrate compliance. It is also signed by Varian’s
Authorized Representative in the EU, and by the
representative of the manufacturing plant.

18 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

1. Introduction

1.1 Pre-installation Requirements

Prior to receiving your instrument, you will have been provided with
a 900-LC Series Liquid Chromatograph Pre-installation Manual
(publication number 8510243900), which describes the
environmental and operating requirements of the LC system. You
must prepare your laboratory according to these instructions
before the LC system can be installed. You should keep the pre­installation manual for future reference. If you have misplaced your
copy, you can obtain a free replacement from your local Varian
office. Alternatively, download a PDF from the Varian, Inc. Web
site, www.varianinc.com

1.2 User Documentation

You have been provided with the following documentation to help
you set up and operate your Varian Integrated Liquid
Chromatograph:

• This Getting Started manual, with safety practices and hazards

information, where to find information about installing and
maintaining instrument components and a brief operation
overview.

• An extensive online Help system containing context-sensitive

help, step-by-step instructions for frequently performed
operations and instructions for using any accessories you ordered.

1.2.1 Conventions

The following conventions have been used throughout the
documentation:

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Varian 900-LC Series Liquid Chromatograph

• Menus, menu options and field names (e.g. select Copy from the
Edit menu) have been typed in bold. Bold is also used to signify
the pushbuttons appearing throughout the software (e.g. click OK).

• ALL CAPITALS indicate keyboard and mouse commands (e.g.
press the F2 key) and text you must type in from the keyboard
(e.g. type SETUP at the prompt).

1.2.2 Notes and Hot Tips

A Note is used to give advice or information. A Note is denoted by
the following symbol: ✒

A Hot Tip is used to give practical hints to help you achieve the
best possible performance from your instrument. A Hot Tip is
denoted by the following symbol: ✰✰✰

1.3 Specifications

The instrument is suitable for indoor use only and is classified

suitable under the following categories (EN 61010-1):

Installation category II
Pollution degree 2
Safety class 1

1.3.1 Environmental Conditions

Condition Altitude Temp

(°C)

Non-operating
(Transport)

Non-operating &
meeting dielectric
strength tests

Operating within
specifications

Table 1. Environmental conditions

20 Publication date: December 2007

0–3 050 m
(0–10 000 ft)

Sea level 40 90–95

0–2 000 m
(0–6 560 ft)

5–45 20–80

10–35 8–80

Humidity (%RH)
non-condensing

Varian 900-LC Series Liquid Chromatograph

✒ Note For optimum analytical performance, it is recommended that the

ambient temperature of the laboratory be between 20 and 25 °C and
be held constant to within ±2 °C throughout the entire working day.

1.3.2 Electrical Power Supplies

All power supplies should be single phase AC, 3 wire system
(active, neutral, ground or two active and ground) and should be
terminated at an appropriate connection receptacle that is within
reach of the system power cable.

1.3.2.1

900-LC Series HPLC System Power Connections

Power supply phase

Power supply, current rating and
overload protection

Australia

USA

Canada

Europe

Plug supplied Required wall socket

Complies with AS3112 To comply with AS3112

Complies with Nema 5-15P To comply with Nema 5-15R

Complies with Nema 5-15P

Complies with CS 22.2 No.
42

Complies with CEE 7 Sheet
VII

Single

Between 3 and 5 A (for 220-240 V
countries)
Between 6 and 12 A (for 110-120 V
countries)

HPM 787 or Clipsal 2015 (250 V,
10 A)

Hubbell 5262 (125 V, 15 A)

To comply with Nema 5-15R

Hubbell 5262 (125 V, 15 A)

To comply with CEE 7 Sheet VII
(250 V, 10/16 A)

Table 2. Power connections

1.3.2.2

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Fuses

1FS1 & 1FS2

T10 A H250 V, 3AB SLO-BLO (for 220-240 V countries)

Varian 900-LC Series Liquid Chromatograph

T20 A H250 V, 3AB SLO-BLO (for 100-120 V countries)

FS3

Table 3. Fuses

✒ Note For safety reasons, any other internal fuse or circuit breaker is not

operator accessible, and should only be replaced by Varian-authorized
personnel.

Fuse information on the rear of the instrument is the most up to
date.

1.3.2.3

Electrical Requirements

The installation of electrical power supplies must comply with the
rules and/or regulations imposed by the local authorities
responsible for the use of electrical energy in the workplace.

All power supplies for the integrated liquid chromatograph and
accessories should be single phase, AC, 3 wire systems (active,
neutral, ground or two active and ground). Each connection should
be terminated at an appropriate receptacle within reach of each
assembly’s power cable. Use of power boards or extension cables

is not recommended.

It is recommended that separate mains circuits, (individually
protected by fuses or circuit breakers) are used for each
component in the system such as the integrated liquid
chromatograph, PC and printer.

T2.5 A H250 V, 5x20 mm (for both 100-120 V and 220-240
V countries)

Avoid using power supplies from a source that may be subject to
electrical interference from other services (such as large electric
motors, elevators, welders and air conditioning units).

The Varian 900-LC Series Liquid Chromatographs are supplied
with a 2 meter long (6 ft 6 in), mains supply cable.

System unit Required supply voltage Nominal rating

920-LC Analytical Liquid Chromatograph 100-120, 220-240 V AC, 50-60 Hz 1000 VA

940-LC Semi-Preparative Liquid
Chromatograph

940-LC Preparative Liquid Chromatograph 100-120, 220-240 V AC, 50-60 Hz 1200 VA

22 Publication date: December 2007

100-120, 220-240 V AC, 50-60 Hz 800 VA

Varian 900-LC Series Liquid Chromatograph

Fraction Collector

ELSD Detector 115-230 V AC, 50-60 Hz 480 VA

External RI Detector 115-230 V AC, 50-60 Hz 150 VA

Ethernet Switch 115-230 V AC, 50-60 Hz 7 VA

Personal computer (Typical) 115, 120, 220, 240 VAC 300 VA

Printer (Typical) 115, 120, 220, 240 VAC 85 VA

Table 4. Power requirements

115-230 V AC, 50-60 Hz 264 VA

1.3.3 Gas Supplies

The installation of compressed or liquid gas supplies must comply
with the rules and/or regulations imposed by the local authorities
responsible for such use in the workplace.

The main gas supply requirement is nitrogen for supply to ELS
Detector. Nitrogen from a cylinder or nitrogen generator may be
used with Varian 900-LC Series Liquid Chromatograph ELS
Detector. Helium may also be required to if you are using a helium
sparging system to degas solvents. Consult the documentation
that came with your helium sparging system for gas requirements.

Nitrogen Gas

Purity

Permissible pressure
range

Maximum pressure

Required flow rates

1.3.3.1

Publication date: December 2007 23

Storage Cylinder Instructions

Cylinders containing gas under pressure should be firmly secured
to a rigid structure, and the storage area must be adequately
ventilated.

Never locate gas cylinders near a source of ignition, or in a
position that is subject to direct heat. Gas storage cylinders often
incorporate a pressure release device which will discharge the gas
at a predetermined temperature, usually around 52 °C (125 °F).

>98% filtered to 0.2 μm

60 to 100 psi
(4–6.7 bar)

100 psi (6.7 bar) regulated

Up to 3.25 L/min at 60 psi at 25 °C

Varian 900-LC Series Liquid Chromatograph

If gases are to be plumbed from a remote storage area to the
instrument site, ensure that the local outlets are fitted with stop
valves, pressure gauges and suitable regulators which are easily
accessible to the instrument operator. The gas outlets must be
provided within 1.5 metres (5 feet) of the instrument.

1.3.4 Waste Container

The Varian 900-LC Series Liquid Chromatograph needs a drain
vessel for disposal of fluids and vapors from the system. Suitable
tubing is supplied with the LC system for use with most HPLC
solvents. Always check that your LC system tubing is compatible
with your solvents.

A chemically inert container, not glass or of a narrow-necked style,
to hold a minimum of 2 liters (4 pints) of waste must be provided by
the user. It should be placed where it is protected by the bench
and in full view of the operator.

1.3.5 Laboratory Conditions

The area selected for the operation of a Varian LC system must
be free from drafts, corrosive atmospheres and vibration.

Sample preparation areas and materials storage facilities should
be located in a separate room.

The area should be a dust-free, low humidity environment.
Air-conditioning is strongly recommended for control of the
environment. For optimum analytical performance it is
recommended that the ambient temperature of the laboratory is
between 20 and 25 °C (68 and 77 °F).

1.3.6 Instrument Cooling Air Supply

The Varian 900-LC Series Liquid Chromatograph requires clean,
dry, non-corrosive air for cooling purposes. This is supplied to

the instrument through an air supply vent located at the rear of the
instrument.

The air supply is used to cool the electronics of the instrument.
Several of these assemblies contain parts prone to corrosion. The
introduction of cooling air contaminated with high levels of acid,
vapors or other corrosive substances may cause damage to the
instrument.

Due to the corrosive nature of some work, it is recommended that

24 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

in applications demanding high usage of corrosive materials, an

external cooling air supply system is provided. It is strongly
recommended that the cooling air be supplied from an

environmentally controlled area that is away from the instrument
exhaust and any other area where corrosive materials are stored
or used.

The cooling air system with flue, fan, ducting and supply cowl,
must provide a minimum positive flow of 6 cubic meters per minute
(200 cfm). The ducting should be corrosion-resistant and fireproof.

1.3.7 Weights and Dimensions

System unit Width Depth Height Weight

Varian 920-LC Analytical Liquid
Chromatograph

Varian 940-LC Semi-Preparative Liquid
Chromatograph

Varian 940-LC Preparative Liquid
Chromatograph

Shipping dimensions for all LC systems

IBM® compatible (typical)

Laser (typical)

External Options

Fraction Collector

Shipping dimensions 760 mm

Refractive Index Detector

750 mm
30 in

750 mm
30 in

750 mm
30 in

950 mm
38 in

450 mm
18 in

500 mm
18 in

400 mm
16 in

30 in

296 mm
12 in

725 mm
29 in

725 mm
29 in

725 mm
29 in

895 mm
36 in

770 mm
30 in

650 mm
30 in

600 mm
24 in

500 mm
20 in

385 mm
15 in

665 mm
27 in

665 mm
27 in

665 mm
27 in

1165 mm
46 in

520 mm
20 in

200 mm
20 in

480 mm
19 in

600 mm
24 in

212 mm
9 in

133 kg
294 lb

139 kg
307 lb

137 kg
302 lb

170 kg
375 lb

(n/a)

(n/a)

18 kg
34 lb

31 kg
69 lb

11 kg
25 lb

Shipping dimensions 775 mm

31 in

Evaporative Light Scattering Detector

Publication date: December 2007 25

200 mm 360 mm 415 mm 11 kg

475 mm
19 in

460 mm
18 in

14 kg
31 lb

Varian 900-LC Series Liquid Chromatograph

(ELSD)

Shipping dimensions 700 mm

Table 5. 900-LC Series Liquid Chromatograph and options dimensions

Warning

The LC system weighs approximately 140 kg (309 lb). To avoid
injury to personnel or damage to equipment, always use a forklift
or other suitable lifting device when moving the instrument.

8 in 14 in 17 in 25 lb

450 mm

28 in

18 in

1.4 Personal Computer Requirements

The recommended and minimum PC specifications are listed in the
following table. They can also be found on Varian’s Web site at
www.varianinc.com. The minimum configuration represents the
absolute minimum that is required to run the Galaxie™ software.
This PC configuration may be out of manufacture, but you may
want to use a PC you already have. The recommended
configuration should be followed when buying a new PC.

Minimum Recommended

600 mm
24 in

18 kg
40 lb

Pentium 4 processor, 2.8 GHz or higher Core 2 Duo, 2.13 GHz or higher

1 GB of RAM 2 GB of RAM

160 GB hard drive 2 x 200 GB hard drives

16 speed CD-ROM or DVD-RW 16 speed CD-ROM or DVD-RW

Super VGA monitor with high color (16
bit) display, 1024 x 768 resolution,
minimum 17” monitor

For the use of PDA features, the
graphic card should be in the list
below:

• INTEL integrated video card

• AM Radeon AGP

• ATI Mobility 128 AGP

• ATI Rage Mobility 128 AGP

• ATI mobility M3

• ATI 3D Rage PRO AGP 2X

26 Publication date: December 2007

Super VGA monitor with high color (16
bit) display, 1024 x 768 resolution,
minimum 17” monitor

For the use of PDA features, the
graphic card should be in the list
below:

• INTEL integrated video card

• AM Radeon AGP

• ATI Mobility 128 AGP

• ATI Rage Mobility 128 AGP

• ATI mobility M3

• ATI 3D Rage PRO AGP 2X

Varian 900-LC Series Liquid Chromatograph

• ATI Rage 128 Pro Ultra GL AGP

• ATI Rage XL PCI (B41)

• ATI Rage 128 Ultra (16MB)

• Neomagic Magicgraph 128XD

• NeoMagic MagicMedia256ZX

• Mga-mil: (chip: MGA-2064WB4R2;

CNA: TI TVP3026 220MHZ)

• Nvidia Riva TNT (with last driver
version: chip: NV4; memory:
16Mo; Files: nv4_mini.sys,
nv4_disp.dll; Version : 4.00,4.0.0)

• NVIDIA GeForce4 MX 420,

DDR

NVidia GEForce4 440 MX

•

Matrox G200 AGP

•

64MB

• ATI Rage 128 Pro Ultra GL AGP

• ATI Rage XL PCI (B41)

• ATI Rage 128 Ultra (16MB)

• Neomagic Magicgraph 128XD

• NeoMagic MagicMedia256ZX

• Mga-mil: (chip: MGA-2064WB4R2;

CNA: TI TVP3026 220MHZ)

• Nvidia Riva TNT (with last driver
version: chip: NV4; memory:
16Mo; Files: nv4_mini.sys,
nv4_disp.dll; Version : 4.00,4.0.0)

• NVIDIA GeForce4 MX 420,

DDR

NVidia GEForce4 440 MX

•

Matrox G200 AGP

•

64MB

16 bit sound card 16 bit sound card

Windows® compatible keyboard Windows® compatible keyboard

Microsoft® or compatible mouse Microsoft® or compatible mouse

Two PCI-compatible slot for I/O card Two PCI-compatible slot for I/O card

Windows® 2000 Windows® XP SP 1

Microsoft® Internet Explorer® v6.0 Microsoft® Internet Explorer® v6.0

Table 6. Recommended PC specifications

*For Galaxie™ CDS server specifications please contact your local
Varian sales representative.

PCs supplied with Letter of Credit orders will be an international
brand and will be the ‘Recommended’ configuration or better.

Higher rated PC components can be substituted for those listed
above e.g., processor type, amount of memory, screen size and
resolution, operating system version etc.

For the latest PC recommendations please see the Varian, Inc.
website at www.varianinc.com

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Varian 900-LC Series Liquid Chromatograph

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28 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

2. Installation

The Varian 900-LC Series Liquid Chromatograph must be installed
by a Varian-trained, Varian-qualified or Varian-authorized
representative.

You should have completed and returned the form in the
pre-installation manual stating that you have prepared the
laboratory in accordance with the requirements detailed in that
manual. A Varian representative will then arrange a suitable
installation date with you.

Details for unpacking the instrument and what to do in case it has
been damaged in transit are also outlined in the pre-installation
manual.

Basic installation involves:

• Setting up the PC to instrument communications

• Installing the Galaxie™ software

• Installing the Online Help

• Installing the hardware

• Running the Installation Qualification tests

• Basic customer training

2.1 900-LC Series LC System to PC Communications

The company network must allow TCP/IP communications
between the client, acquisition server and HPLC systems.
However a desktop switch is provided with the instrument, to allow
local networking. The following are suggested network connections
using the provided switch:

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Varian 900-LC Series Liquid Chromatograph

Figure 1. Single 900-LC Series LC System in standalone configuration

Figure 2. Multiple 900-LC Series LC Systems in standalone configuration

Up to four systems can be controlled by one Galaxie Acquisition
Server. For a client server system there will need to be a PC with
the Galaxie Acquisition Server running for every four HPLC

30 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

systems.

Figure 3. Networked multiple 900-LC Series LC System configuration option 1

Figure 4. Networked multiple 900-LC Series LC System configuration option 2

You or your network administrator must provide any hardware,
adapters, cables, and configuration and setup work required to

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Varian 900-LC Series Liquid Chromatograph

convert from the company network wiring type to 10/100 BaseT
wiring. This hardware must comply with the IEEE 802.3 standard.

Choose the appropriate set up for your laboratory. Use standard
Ethernet cables. Your 900-LC Series HPLC system came with one
Ethernet cable. You will need to provide additional cables based
on your LC system and PC configuration.

To connect the Ethernet cables:

1. Connect an Ethernet cable from the Network/PC port on the
side of the 900-LC Series HPLC system to the provided
Ethernet switch. Consult the instructions provided with the
switch for more information.

Figure 5. Side of 900-LC Series HPLC

This image has been flipped to show horizontal. The connections are
vertically oriented on the side of the LC system.

2. Connect an Ethernet cable from the Ethernet switch to the
Ethernet port on the PC(s) and/or company network.

2.2 Installing the Galaxie™ Chromatography Data System

Varian recommends that you purchase a PC as part of the Varian
900-LC Series Liquid Chromatograph package. The PC included in
the package will come with the appropriate operating system as
loaded by the PC supplier.

For instructions on installing the Windows® operating system,
please refer to the appropriate manuals supplied with the software.
It is your responsibility to ensure that the operating system
software has been installed and is functional if you are supplying
the PC or the operating system.

✒ Note Varian will not assume responsibility for loss of data.

Your Varian representative will connect the PC to the LC system
and any factory-approved accessories purchased at installation.
Initial instrument software installation is also included as part of the

32 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

system installation.

Although the Varian-trained, Varian-qualified or Varian-authorized
representative will install the Galaxie™ software for you during the
installation process, you may need to install the software yourself
at some later stage, for example if you change the PC.

The Galaxie LC Drivers Serial Number will also work for the 900­LC. If you are running both types of Varian LC systems, the same
serial number will need to be entered twice, both when installing
LC Drivers and when installing the 900-LC driver. You will only
need to purchase the one serial number. For subsequent installs,
the serial number is recognized.

2.2.1 Client/Server Installation Information

The client/server installation involves using up to three PCs:

• Main server – where data and methods are stored.

• Acquisition server – communicates with the hardware of the

900-LC Series HPLC system. Up to four 900-LC systems can
be run by one acquisition server. The system is configured on
the acquisition server.

• Client – methods are created, data viewed and reprocessed.

✒ Note The 900-LC Series driver must be installed on all three PCs. The

online help must be installed on every PC where it may need to be
viewed on.

2.2.2 Stand-alone Installation Information

When using a stand-alone configuration the software for the main
server, acquisition server and client are installed on one PC. This
PC can then be used to control up to four 900-LC Series HPLC
systems.

2.2.3 Galaxie Installation Procedure

The 900-LC Series Driver installation has several steps:

1. Install the Galaxie core software.

2. Install LC or GC drivers if required.

3. Install the 900-LC Series HPLC driver.

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Varian 900-LC Series Liquid Chromatograph

4. Install the 900-LC Series HPLC Online Help.

5. Configure the 900-LC Series HPLC system(s).

✒ Note Before installation you must have the LC system set up and turned

on. Set up includes having the network cabling and hardware
plugged in.

Contact your IT department to determine if you will be setting up
the instrument as a dynamic or static IP and if you will be setting
up a client/server system or a standalone system.

✒ Note The dynamic IP address must be leased to the instrument and the

static or dynamic IP address of the LC system must be on the
same gateway/subnet as the acquisition server or stand-alone PC.
In addition, the LC systems must be connected to the provided
Ethernet switch.

✒ Note Disable the Windows® firewall on the PC(s) to be used with

Galaxie software. This will help prevent installation and data
collection problems.

Prior to installation check the following:

• Disable “Hyperthreading in the Bios. Consult your PC manual
for instructions.

• Check that power management of your Ethernet network card
is set to never allow the card to be turned off. Consult your PC
manual for instructions.

• In the Windows® Control Panel > Display > Screen Saver set
the power scheme for your monitor and hard drive to Never.

• Turn off the screen saver.

• Turn the Windows® XP Pro sp2 firewall off.

• Disable any virus scans.

• Turn off Automatic Updates in the Windows® Control Panel.

2.2.3.1

✒ Note The 900-LC Series HPLC Driver requires Galaxie™ software

34 Publication date: December 2007

Installing the Galaxie Core Software

To install the Galaxie™ core software:

1. Please see the Galaxie Chromatography Data System Installation

Guide, p/n 03-914948-02 for installation instructions.

version 1.9 sp1.

Varian 900-LC Series Liquid Chromatograph

2.2.3.2

2.2.3.3

✒ Note Before installation you must have the LC system set up and turned

Installing Other LC or GC Device Drivers

The drivers work with the Galaxie core software to provide instrument
control and data acquisition. Go to the next section, Installing the
900-LC Series HPLC Driver, if this section is not required.

To install other LC or GC device drivers:

1. Please see the Galaxie Chromatography Data System Installation
Guide, p/n 03-914948-02 for installation instructions.

Installing the 900-LC Series HPLC Driver

The drivers work with the Galaxie core software to provide instrument
control and data acquisition. Install this driver on every Main Server,
Acquisition Server and Client PC that will be working with the 900­LC Series HPLC system.

on. Set up includes having the network cabling and hardware
plugged in.

To install the 900-LC Series HPLC Driver:

1. Insert the 900-LC Series Galaxie Driver CD into your CD-ROM
drive. The software will automatically start

2. Click Next on the Welcome to Install Shield Wizard for Varian
Series 900-LC Drivers window.

3. Click Yes to accept to the License agreement. Clicking no will
terminate the program.

4. Enter in your name, the company name, and the serial number of
the 900-LC Series HPLC Driver Disk. The serial number is
provided on your driver serial number card and is different from
the Galaxie serial number.

The installer begins to install the driver files onto the PC.

5. Click Yes to install the Flash Player.

1

.

✒ Note If you click No, you will not get the full functionality of the 900-LC

Series HPLC driver; however, Flash is not required to run the 900-LC.

1

If you have turned off the auto-start capability of your CD-ROM drive, you will need to run the setup.exe file on the

CD-ROM.

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Varian 900-LC Series Liquid Chromatograph

6. Click Close on the Flash Player 9 Installer Setup: Completed

window to continue the driver installation.

7. Click Yes to install Adobe Acrobat Reader.

✒ Note If you click No, installing Adobe Acrobat Reader will be skipped.

However, you may not be able to view any accompanying PDF files
in the help.

If you click Yes and the Repair/Modify window appears, Adobe
Acrobat Reader is already installed on your PC. Click Cancel to stop
the Adobe Acrobat Reader installation or click Repair to re-install.

8. Click Finish to complete the Adobe Acrobat Reader installation.

2.2.3.4

2.2.3.5

Installing the 900-LC Series HPLC Help

This step is a continuation from the previous section, Installing the
900-LC Series HPLC Driver.

To install the 900-LC Series HPLC Help:

1. Remove the driver CD and insert the 900-LC Series HPLC Help

CD when instructed. Click OK. If you click Cancel, a message will
appear confirming your choice not to install the Online Help.
Click No to exit the installation or click Yes to continue
installing the Online Help.

2. Click OK to continue the installation.

3. Click Next on the Welcome to the Varian 900-LC Series HPLC

Help Setup Wizard window.

4. Click OK to accept the default installation location or choose a

different location to install a shortcut to the Online Help.

5. Click on Finished.

Configuring the 900-LC Series HPLC System(s)

This step is a continuation from the previous section, Installing the
900-LC Series HPLC Help. To manually configure an LC system,
please see the Galaxie Configuration Manager online help or
access this set up program by navigating to
C:\Galaxie\Server\Devices\SetupWizard.exe. Double click
SetupWizard.exe to launch the setup program.

To access the Galaxie Configuration Manager Online Help:
Click the Windows® Start button and choose Programs >

Galaxie™ (Chromatography) > Help > Galaxie™ Configuration
Manager.

36 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

Systems should only be configured on an acquisition server or a
stand-alone PC configuration.

To configure the 900-LC Series HPLC System(s)

1. Click Yes to configure a system if you are installing on an
Acquisition server or on a Stand-Alone system. If you choose
No, the installer will exit.

2. Click Yes or No to the question ‘Do you want to give the right
“Allow to run instrument maintenance software’ to ALL of the
Galaxie user profiles?’. This will provide access to the Instrument
Maintenance Software (IMS) for all profiles. See page

42 for

more information on IMS.

✒ Note To configure IMS access for individual profiles use Galaxie

Configuration Manager.

3. If Yes was clicked in step 2, click OK at the user profile changed
notification.

4. The 900-LC Series Setup Wizard window appears. Enter in the
name of the system.

✒ Note Ensure you have typed in the correct system name. To modify a

system name later you must first delete that LC system and then
use this SetupWizard.exe to recreate the LC system.

5. Click on the magnifying glass icon

to start the system

discovery process. This can take up to 5-10 seconds.

6. Click the down arrow button in the Instrument field to select the
LC system.

✒ Note Identify the system by the MAC address which can be found on

the back of the LC system and in your documentation that came
with the LC system.

Once the system is chosen, IP address information will be
automatically entered into the IP address, Subnet mask, and Default
gateway fields. This information will be what the instrument was
shipped with and may not be appropriate for your use.

7. Choose Obtain an IP address automatically (DHCP) or Use a
static IP address.

(a) When Obtain IP address automatically (DHCP) is chosen,

the system will search for an IP address automatically. If
one is not found, click Retry. The Switching to DHCP mode
window pops up. Click Initialize instrument. This will

Publication date: December 2007 37

Varian 900-LC Series Liquid Chromatograph

reboot the LC system communications card. Click Close

when the initialization is complete.

✒ Note If an IP address still does not appear, contact your IT department

to check your network connections. The LC systems must be on
the same gateway/subnet as the acquisition server or stand-alone
PC configuration and be connected to the provided Ethernet
switch.

(b) When Use a static IP address is chosen, enter in the IP

address, Subnet mask, and Default gateway for the LC
system. The LC systems must be on the same
gateway/subnet as the acquisition server or stand-alone PC
configuration and be connected to the provided Ethernet
switch.

8. Click Create.

9. Click OK when the message confirming the system creation

comes up.

10. Create up to three more systems or click Close to exit the

installer.

2.2.4 Delete a System

If the system needs to be renamed or the IP address changed you
will need to delete the existing system and recreate it.

To delete a system:

1. Open Galaxie Configuration Manager.

2. Click the Windows® Start button and choose Programs >
Galaxie™ > Galaxie™ Configuration Manager.

3. Log in to Galaxie Configuration Manager.

4. Click Systems in the upper left side of the window.

5. Right click the system to be deleted and choose Delete.

6. Click Communication engine configuration on the lower left
side of the window.

7. Highlight the first line with the system name to be deleted and
then click Remove on the upper right side of the window.

8. Repeat the previous step for all lines with the system name to be
deleted.

9. Click Apply.

38 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

10. Click Interface configuration on the bottom left side of the

window.

11. Highlight the first line with the system name to be deleted and

then click Remove on the upper right side of the window.

12. Repeat the previous step for all lines with the system name to be
deleted.

13. Click Apply.

14. Exit Galaxie Configuration Manager.

2.2.5 Change the 900-LC Series HPLC System Name or IP

Address

You cannot change the name or IP address of an LC system. You must
delete the system first and then re-create it using the
SetupWizard.exe.

To change the name or IP address of a system:

1. Delete the LC system. See page 38 for instructions.

2. Re-create the LC system. See page 36 for instructions.

2.2.6 Update Galaxie to Version 1.9 SP1

The 900-LC Series HPLC Driver requires Galaxie version 1.9 sp1 to
run. The ordering information for the CD is part number 03-950000­98 (Galaxie Software Service Pack 1 (SP1) CD).

✒ Note Contact your local Varian representative to update Galaxie version

1.8 to 1.9 SP1. This CD will not update Galaxie version 1.8 to 1.9

SP1.

To update to version 1.9 sp1:

1. Insert the CD into your CD-ROM drive.

2. Click Next on the Welcome screen.

The updater will install and update the required files.

3. Click Yes to restart your PC.

4. Click Finish to restart the PC.

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Varian 900-LC Series Liquid Chromatograph

When the restart has completed a window will appear asking for the
Main server name.

5. Enter the main server name into the field.

✰✰✰ Hot Tip To familiarize yourself with the

click the Windows® Start button and choose Programs > Galaxie™ >
Galaxie™ Help.

Galaxie™ software, after installation,

2.3 About the Varian Integrated HPLC Online Help

Throughout this manual references are made to the Varian 900-LC
Series LC online help or Galaxie™ Chromatography Data Systems
help.

The Varian LC system online help is provided on a separate CD to
the Galaxie™ software and is installed on your PC during the
Galaxie™ software installation.

The LC system help includes information and instructions on:

• Safety

• LC system standard and optional hardware

• Installation of the LC system options and components

including the:

• hardware

• LC system driver for the Galaxie™ software

• Creating methods

• Viewing run details

• Saving the run

• Maintenance

• Troubleshooting

• Spares and options

40 Publication date: December 2007

2.4 Hardware Installation

Your LC system should be ready to operate after the Varian
representative has installed it. However, over time you may need
to replace some hardware components such as detector lamps,
autosampler needles, etc or may need to install a new device to
your LC system. For instructions on how to do this, please refer to
the online help. Specifically:

1. Click the Windows® Start button and choose Programs >
Galaxie™ > Help > Varian Integrated Help.

2. When the Integrated Help appears, click the How to > Installing
Components link to access step-by-step instructions on how to

remove and install the various instrument components.

For information on your instrument connections including
connecting the network and communication cables please refer to
the online help.

1. Click the Windows® Start button and choose Programs >
Galaxie™ > Help > Varian Integrated Help.

2. When the Integrated Help appears, click About Your Instrument
link to access information about the front, side and back
connections and components of your LC system.

Varian 900-LC Series Liquid Chromatograph

2.5 Installation Qualification Tests

Your Varian customer service representative will perform the
Installation Qualification tests during the installation procedure.

You will receive a copy of the results of the tests. It is
recommended you save those results as a record of your
instrument performance at installation.

2.6 Customer Training

During the installation process you will receive basic training on
how to:

• set up the hardware

• use the Galaxie™ software

• use the Online Help

• use the IMS software.

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Varian 900-LC Series Liquid Chromatograph

2.7 Using the Online Help

The online help is context sensitive. Pressing the F1 key on any
screen in the device control section of the Galaxie™ software will
bring up help that relates to that screen.

Alternatively, you can access the online help by:

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

4. Click Help.

5. Click Varian Integrated Help.

2.8 Instrument Maintenance Software (IMS)

IMS software is used to keep track of any maintenance performed
on the LC system and usage over time of components within each
device in your LC system.

IMS has several features:

• Counters which record component usage

• Actions which lists all procedures that can be tracked

• The Log Book lists all actions and counter information and can

be saved in a PDF file

• Service tests which can test the performance of devices in the
LC system.

2.8.1 How to Access IMS

Access the Instrument Maintenance Software from the link on the
right side of the Status Overview window in the Galaxie™
software. The Instrument Maintenance software can be run with
the Galaxie™ version 1.9 SP1 or later. When the logon window
appears, enter a User identification, and choose the group and
project in which the system to be maintained is defined in Galaxie.
Type the corresponding password. The password is case sensitive
(abc ≠ ABC).

To be able to log into the Instrument Maintenance Software, 'allow
to run instrument maintenance software' must be checked in the
Acquisition/system properties part of the user profile. If the user

42 Publication date: December 2007

does not have the right to run maintenance software an error
message is displayed.

2.8.2 About IMS

The software has the menu bar at the top which includes:

• File

Varian 900-LC Series Liquid Chromatograph

• Open Audit trail

• Save Audit trail

• Preview Audit trail

• Print Audit trail

• Quit

• View

• Actions

• Maintenance mode ON

• Maintenance mode OFF

• About

For detailed information about the IMS software please see the
900-LC Series HPLC online help.

To access the online help:

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

4. Click Help.

5. Click Varian Integrated Help.

6. Click Instrument Maintenance Software in the online help

table of contents.

For information on audit trails:

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

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Varian 900-LC Series Liquid Chromatograph

4. Click Help.

5. Click Varian Integrated Help.

6. Click the plus sign next to Instrument Maintenance
Software in the online help table of contents.

7. Click Audit Trails.

44 Publication date: December 2007

Varian 900-LC Series Liquid Chromatograph

3. Operation

You will find step-by-step instructions for common operations in the
Integrated HPLC Help. To access this information:

1. Select the Windows® Start button and choose Programs >
Galaxie™ > Help > Varian Integrated Help.

2. When the Integrated HPLC Help appears, click the How to link to
view the available step-by-step instructions.

Below is a quick guide to getting the instrument set up and running
samples.

3.1 Analysis Checklist

You need to complete the following steps in turn to measure a
sample/s. You will find details on each step in this chapter.

• Turn on the instrument and software

• Prepare the instrument for use

• Configure the Device Settings and System Parameters

• Create a method

• Run samples

• View or Print a report

3.2 Turning on the Instrument and Software

Before starting the system, carefully read the Safety practices and
hazards
laboratory is set up according to the details specified in the
Pre-installation manual.

To turn on the instrument and software:

1. Switch on the computer, monitor and printer.

Publication date: December 2007 45

section at the front of this manual and ensure that the

Varian 900-LC Series Liquid Chromatograph

2. Turn on the LC system.

3. To start the Galaxie™ software, click the Windows® Start button
and then choose Programs > Galaxie™ > Galaxie™.

4. Enter in your login details and click OK.

3.2.1 Connecting to a System

Depending on the configuration you purchased, you may have up
to four LC systems connected to one PC. Due to this flexibility in
the software, you must connect to a system when you open the
Galaxie™ program.

To connect to a system:

1. Click the Systems tab at the bottom left of the main Galaxie™
window.

2. Select the empty box next to your system.

Your Galaxie™ software will automatically begin communicating
with your LC system.

✒ Note There will be a delay between choosing your system and the system

information appearing on your screen. The Galaxie™ software is
communicating with the LC system.

3.3 Preparing the System for Use

For consistent and reproducible results with each use of the iHPLC
it is recommended that you follow this procedure to prepare your
system to run samples.

To prepare the system for use:

1. Check that all tubing to the column(s), pumps, and waste is
correctly connected and in the proper position.

2. Place all samples into the autosampler tray. Cool the tray prior to
placing samples into it if necessary.

3. Close the LC system doors.

4. Check that all tubes are placed in the fraction collector (if
necessary).

5. Set the flow rate manually to remove any air bubbles or old
solvent from the system.

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6. Purge the pumps if new solvent is being used.

7. Purge any air bubbles from the autosampler injection valve if
necessary.

8. Turn on the column heater and allow the column(s) to come to
the correct temperature if necessary.

Caution

Caution

It is imperative that whenever the column heater is turned on, the
pump should be running with a non-absorbing solvent flowing
through the column to avoid column degradation.

9. Turn the detector lamp(s) on.

It is imperative that whenever the Varian detector lamp is turned
on, the pump should be running with a non-absorbing solvent
flowing through the cell, with no air bubbles present.

For more information on getting your system prepared for use
please see the 900-LC Series HPLC Help.

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

4. Click Help.

5. Click Varian Integrated Help.

6. Click the plus sign next to Getting Started.

7. Click Preparing the LC System For Use.

3.4 Configuring the Device Settings and System Parameters

The Device Settings allow you to program settings for each
component within your iHPLC as well as control flow rates, purge
the pumps, turn the detector lamps on, heat the column oven and
move the autosampler needle and fraction collector probe.

The System Parameters are settings that will download directly

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your method. They include information such as the pressure units
to be displayed on screen, autosampler tray size, fraction collector
rack sizes, leak detection actions, pump head size, sample loop
size, and enabling the cooling on the autosampler trays.

For additional information on your system components please see
the 900-LC Series HPLC Online Help.

3.4.1 Flows

In Flows you can:

• manually set and turn on your flow rate and solvent
composition

• purge the pumps

• set the bottle management parameters

• select the solvents for the scale up module valves (if present

in your system)

3.4.2 Detectors

Depending on the detector you purchased, in the detector Device
Settings you can:

• choose wavelengths

• autozero the detectors

• turn your detector lamp(s) on

• choose the data collection parameters

• set the detector temperature conditions.

3.4.3 Column

Depending on the column options you purchased you can:

• set the column heater temperature

• turn the heater on

• choose the scale up module column

• choose between two columns if a column switching valve is

present

• view the column properties of your column if you have

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downloaded your method to the LC system.

3.4.4 Sample Introduction

Sample introduction can occur by using an autosampler or manual
injector if you are running an analytical or analytical to semi­preparative LC system or via a manual injector or pump if you are
running a preparative scale LC system.

Autosampler

If you purchased an analytical or analytical to semi preparative
system you may have an autosampler.

In the Autosampler Device Settings you can move the needle to
the:

• wash port

• injection port.

You can also flush the syringe barrel to replace solvent or remove
bubbles by clicking the Flush Syringe Barrel button.

If you have the cooling option on your autosampler you can
change the temperature.

Varian 900-LC Series Liquid Chromatograph

Manual Injector

Depending on the options you chose, you may have a manual
injector. This allows you to make single injections. The Device
Settings window lets you make an injection and shows you the
state of the injector.

Injection Pump

The preparative scale LC system may have an injection pump. In
the injection pump method you can program the injection volume
and flow rate.

3.4.5 System Parameters

System Parameters are settings that affect new methods. If you
change the System Parameters while a method is already open,
the changes will not affect that method.

Depending on the system you purchased you can:

• set the units preferred for the pressure and the cell ratio if
using a semi prep or prep cell

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Varian 900-LC Series Liquid Chromatograph

• set the option to turn the lamps on as the detector(s) power up

• select normalization for the PDA detector

• synchronize with other modules can be selected to have the

system wait for when a contact closure is to be used to start
data acquisition

• choose the tray type and cooling options in the autosampler
section

• edit the manual injector options including the reset time,
analytical (SUM) loop size and preparative loop size

• edit the reset time for a manual injector which is used to
switch the valve back into the idle position

• choose which leak detector option will be performed when a
leak is detected

• choose solvents to be used in the valve module section if you
have a Scale Up Module

• with semi-prep and prep systems you can choose the pump
head sizes, sample delivery pump head size and pressure
units

• in the fraction collector section you must choose your rack
type, tube volume and probe depth

• save your settings

3.5 Creating a Method

To create a new method:

1. Click File > New > Method in the upper left corner of the

Galaxie™ software.

2. Choose the Project Name and System name from the drop-

down menus and then click Next.

3. Enter the Method Name and Description and then click OK.

✒ Note Galaxie™ will open to the main method screen on the Integration

Events tab.

4. In the lower left corner click on control.

✒ Note Galaxie™ will show the Device Select window. You should see a list of

your system components just to the left of the Device Select window.

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5. Choose the devices needed for your run by selecting or
deselecting the options in the Device Select window.

6. On each device window set your parameters.

✒ Note Your System Parameters will automatically appear in your method.

All other settings will need to be checked.

7. Click acquisition in the lower left corner of the Galaxie™

software.

8. Click on the button with the three dots next to the File prefix
field to open up variable options for your run name. Double
click the variables to be used in your run name or type in a

name for your run and then click OK.

9. Set the identifier number if something other than 1 is required
to name the run.

10. In the Acquisition Parameters section enter in the Vial and
Rack number if an autosampler is to be used, the Acquisition
length and Injection Volume.

✒ Note For information about the additional fields in the Acquisition window

please see your Galaxie™ Chromatography Data System software
Online Help.

11. Click File > Save > Save Method. The method will now be

saved in the default data folder.

For more information on creating a method please see the 900-LC
Series HPLC Help.

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

4. Click Help.

5. Click Varian Integrated Help.

6. Click the plus sign next to Software.

7. Click Galaxie software tutorial for a basic overview of the

main Galaxie windows.

8. Alternatively, click the plus sign next to How To…

9. Click Program a method from start to finish for a step by

step tutorial on how to program a method.

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3.6 Starting an Acquisition

There are two ways to start an acquisition. You can have multiple
injections by creating a sequence or single injections using Quick
Start.

3.6.1 Sequence

1. From the File menu click New > New Sequence.

2. Choose the Project name and System name to be associated with

this sequence and then click Next.

3. Enter the number of lines (number of samples) in the sequence

and then click Next.

4. Enter the new sequence name and a description if needed and

then click OK.

✒ Note A window appears with the number of lines you chose in step 3. You

can auto-fill the columns by entering or choosing a value in the first
line and then right clicking the heading of that column. Choose Fill
Block and then follow the prompts. Alternatively you can manually
fill in each field to customize your sequence.

5. Enter in all required fields.

6. Click File > Save > Save Sequence.

7. On the Sequence page, click the green arrow button just above
and left of the lines.

8. To view your runs click the Systems tab on the bottom left of
the Galaxie™ software.

✒ Note For further information about starting an acquisition and

interpreting the results, refer to the Galaxie™ Chromatography
Data System Online Help.

3.6.2 Quick Start

To start a Quick Start run:

1. Click Acquisition on the top menu bar.

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2. Choose Quick Start.

3. Select the Project name, System name and Method name and

then click OK.

4. Click on the button with the three dots next to the File prefix
field to open up variable options for your run name. Double
click the variables to be used in your run name or type in a

name for your run and then click OK.

5. Set the identifier number if something other than 1 is required
to name the run.

6. In the Acquisition Parameters section enter in the Vial and
Rack number if an autosampler is to be used, the Acquisition
length and Injection Volume.

7. Click the Start button.

8. To view your run click the Systems tab on the bottom left of

the Galaxie™ software.

✒ Note For further information about running samples and interpreting the

results, refer to the Galaxie™ Chromatography Data System
Online Help.

3.7 Printing a report

To print a report:

1. Click Report Style in the lower left corner of the Galaxie™
software.

✒ Note When printing a report, you can simply select Print from the File

menu. However, if you do so, a report will be generated based
upon the last used Report Settings. You will not be able to select
the data to be included in the printout.

2. Choose a report style or edit a style.

✒ Note For more information on how to edit report styles please see the

Galaxie™ Chromatography Data System Online Help.

3. Click File > Print to generate a report as specified.

4. The Print dialog box will open. You can select to print all of the
report, or a range of pages. Reports are printed to your default

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Varian 900-LC Series Liquid Chromatograph

printer, unless you specify otherwise. You can specify your
printer options in the Print Setup dialog box, accessible from the
File menu.

As the Print dialog is a standard Windows® dialog, you can obtain
“What’s this” help on an item by clicking the question mark icon in
the top right corner and then clicking the item of interest.

3.8 Saving Your Files

There are two ways to save a file.

• Save a file with the same name (overwriting the previous one)

by using the menu File > Save / Save file type

• Save the file with another name by using the menu File >
Save As / Save file type As.

In both cases, the Galaxie™ Software prompts as default to save
the file type corresponding to the active file.

The Save file window displays the list of the files already created in
the directory associated with the group (when the user is logged on
to All projects) or with the project (when the user is logged on to a
specific project).

Access the sub-directories by double-clicking on the corresponding
folder icon. Once inside the sub-directory, the path is displayed at
the top of the window.

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4. Maintenance

This chapter includes information on how to access the Varian
900-LC Series HPLC system maintenance requirements that may
be carried out by an operator. Any maintenance procedures not
specifically mentioned should be carried out only by Varian-trained,
Varian-qualified or Varian-authorized representatives.

Warning

The UV-Vis, PDA and Fluorescence detector lamps are intense light
sources. Direct viewing of the light source will cause eye damage.
Operators and other unauthorized personnel must NEVER remove
the main covers.

Warning

This instrument contains electrical circuits, devices and components
operating at dangerous voltages. Contact with these circuits, devices
and components can result in death, cause serious injury, or painful
electrical shock.

Warning

Allow hot parts to cool before proceeding with any maintenance
procedure.

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Warning

There is a danger of ignition when using flammable chemicals such
as organic solvent. Aqueous solvents or organic solvents having an
ignition point above 70 °C can be used. Do not use organic solvents
having an ignition point below 70 °C.

Be careful about ignition due to static electricity when using
flammable chemicals. Use a conductive vessel and carry out
grounding correctly, especially when using a non-conductive
chemical. This instrument is not explosion-proof.

Warning

Always check the flow path for leakage of solvent or waste solution
during use and leakage of solvent inside the instrument. If an
abnormality is found, stop the operation immediately.

Provide sufficient ventilation in the room.

Use of the LC system and accessories may involve materials,
solvents and solutions which are flammable, corrosive, toxic or
otherwise hazardous. Careless, improper or unskilled use of such
materials, solvents and solutions can create explosion hazards, fire
hazards, toxicity and other hazards that can result in death, serious
personal injury or damage to equipment.

✒ Note This section refers only to maintenance procedures for the instrument.

You should refer to your PC and printer manuals for their
maintenance procedures and to the 900-LC Series HPLC Help for the
maintenance procedures for any accessories you ordered.

4.1 Routine Maintenance

Routine maintenance information is provided in the 900-LC Series
HPLC online help. To access these instructions:

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

4. Click Help.

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5. Click Varian Integrated Help.

6. Click the Maintenance link.

7. Click Checklist.

4.2 Cleaning

Any spills in the sample compartment or on the LC system should
be wiped up immediately.

The exterior surfaces of the instrument should be kept clean. All
cleaning should be done with a soft cloth. If necessary, this cloth
can be dampened with water or a mild detergent. Do not use
organic solvents or abrasive cleaning agents.

4.3 Spare Parts

For spare parts and consumables ordering information, refer to the
Varian, Inc. Web site:

www.varianinc.com

Varian 900-LC Series Liquid Chromatograph

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5. Troubleshooting

For troubleshooting help please refer to the Varian 900-LC Series
HPLC online help.

To access the online help troubleshooting section:

1. Click the Windows® Start button.

2. Click Programs.

3. Click Galaxie™.

4. Click Help.

5. Click Varian Integrated Help.

6. Click the Troubleshooting link for general LC system

troubleshooting or click the plus sign next to Troubleshooting
to access the device troubleshooting information.

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