Vanguard 1442MMi Operation Manual

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Printed in the USA VGMNL062512-1400MMi
Operation Manual
Model 1442MMi
www.veegee.com | 800-423-8842
sales@veegee.com | support@veegee.com
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Vanguard 1442MMi Industrial microscopes are suited for brightfield and simple polarization observation of industrial applications such as semiconductor inspection, viewing of metallographic specimens, and crystallographic analysis.
Viewing Head. Trinocular (Seidentopf) head is inclined at 30° and features interpupillary and dioptric adjustments. The trinocular head features a sliding main prism with a 100/0 split.
Eyepieces. 10X ultra-widefield with a field number of 22.
Nosepiece. Quintuple, ball-bearing nosepiece with high-grade
lubricant for smooth operation, and positive stops.
Objectives. The Plan Achromatic, long working distance, infinity corrected objectives are optically coated to reduce unwanted reflections.
Stage. The mechanical stage measures 140mm x 185mm and includes a removable 95mm x 75mm glass insert. Motion of mechanical stage is controlled by a right-hand, low-position coaxial control and is driven by a rack and pinion system.
Focusing movement. Coaxial, ultra-low position coarse and fine focus controls feature a 30mm focusing range and are graduated to 2 microns per division. Fitted with a tension adjustment and stage limit control.
Transmitted Condenser. The 0.85 N.A. Abbe brightfield condenser includes an iris diaphragm and swing-in lens.
Transmitted Illumination. 30W variable quartz halogen light source. Comes with blue and dispersion filters. Also includes filters for simple polarization.
Reflected Illumination. 50W variable quartz halogen light source. Comes with blue, yellow, green, and dispersion filters. Also includes filters for simple polarization.
Body. Cast-metal, ergonomic body with stain-resistant enamel finish.
1442MMi Industrial Microscope
Thank you for purchasing this VanGuard microscope. With the user in mind, VanGuard microscopes are built from modern designs and should provide a lifetime of reliable performance. Before using this microscope it must be properly setup, which requires some familiarity with the microscope. For this reason we recommend you read this entire manual carefully before setting up and using the instrument.
IntroductionIntroduction
IntroductionIntroduction
Introduction
2
See included warranty card for more information.
Disclaimer:
The information provided in this operation manual is believed to be accurate and reliable at the time of printing. However, no responsibility shall be assumed by VEE GEE Scientific for its use. The information contained in this document is subject to change without notification.
This product is designed and intended for use only as a microscope system. Modifying the product in any manner for use not originally intended shall automatically void the manufacturer’s warranty. In no event shall VEE GEE Scientific, Inc. be held liable for any incidental or indirect damage arising from the use of modified or altered product.
All rights reserved. No part of this document may be reproduced or transmitted in any form without the prior written consent of VEE GEE Scientific, Inc.
©2012 VEE GEE Scientific, Inc.
5 Year Limited Warranty
VanGuard Microscopes are warranted by VEE GEE Scientific, Inc. to be free from defects in material and workmanship for a period of five (5) years from the date of purchase, except for electrical components which have a one (1) year limited warranty. During this period, VEE GEE Scientific, or its authorized service station, will at their option and without charge, either repair or replace any part found to be defective in materials or workmanship.
This warranty is subject to the following limitations and exceptions and will not apply if:
1) There is lack of proof of date and place of purchase. The purchase invoice must accom­pany the unit when sent in for repair. The warranty extends to the original consumer pur­chaser only and is not assignable or transferable.
2) The damage is due to normal wear and tear, misuse, abuse, negligence, accident, inad­equate maintenance, disregard for operating instructions, or to any other cause not due to the manufacture of the microscope (i.e. objective failure because of oil penetration due to lack of timely cleaning).
3) The serial numbers, names, and/or functions are altered or obliterated; or unauthorized repair or replacement of parts by the End-User or an unauthorized third party while under warranty.
4) Consumable items (such as, but not limited to bulbs) have failed.
This warranty expressly excludes transportation damage and adjustment or readjustment. In no case shall VEE GEE Scientific be liable to the Buyer or any person for any special, indirect, incidental, or consequential damage whether claims are based in contract or otherwise with respect to or arising out of product furnished hereunder. For goods manufactured by any third party, VEE GEE Scientific’s liability under warranty is limited to the terms of the warranty by the supplier for the goods. All warranty work shall be performed at the authorized service center which is: National Microscope Exchange, Tel: (425) 788-2662. Contact your distribu­tor or NME to discuss the problem and obtain instructions for the return of your microscope for repair. The original purchaser returning this product must prepay all postage, shipping, transportation, packaging, and delivery costs to NME.
www.veegee.com | 800-423-8842
sales@veegee.com | support@veegee.com
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General InformationGeneral Information
General InformationGeneral Information
General Information
3
T able of Contents
Introduction........................................................ 2
Table of Contents.............................................. 3
Safety Information............................................. 3
Components
Microscope Diagram.................................. 4-7
Included Parts................................................ 8
Optional Accessories...................................... 8
Assembly
Unpacking Components................................. 9
Head & Reflected Illuminator Assembly.... 9-10
Objective Installation..................................... 10
Stage Plate Installation.................................. 10
Condenser Installation.................................. 11
Power Cord Installation................................. 11
Setup
Reflected Illumination Setup................... 12-13
Reflected Field Diaphragm Alignment..... 13-14
Eyecups........................................................ 14
Interpupilliary Adjustment.............................. 14
Focus Adjustment.......................................... 15
Focus Tension Adjustment............................. 15
Setup (continued)
Stage Limit...................................................... 15
Diopter Adjustment......................................... 15
Transmitted Illumination Setup................. 16-17
Kohler Illumination Setup......................... 17-18
Trinocular Camera Port Setup........................ 18
Microscope Use
Focusing & Mechanical Stage Mechanisms.... 19
Abbe Condenser Swing-In Lens..................... 19
Colored Filters - Transmitted.......................... 20
Colored Filters - Reflected............................. 20
Polarizer/Analyzer - Transmitted..................... 20
Polarizer/Analyzer - Reflected........................ 20
Maintenance
Replacing the Transmitted Illuminator Lamp... 21
Replacing the Reflected Illuminator Lamp...... 21
Replacing the Fuse......................................... 22
Caring for your 1442MMi Microscope............. 23
Troubleshooting........................................ 24-25
Specifications................................................. 26
Warranty Information..................................... 27
Attention - possible injury or product damage if instructions are not followed
Safety Symbols
Anytime you see the following symbols used in this manual pay special attention and follow the safety precautions.
Hot Surface - potential for burns if contact with hot surfaces are made
Shock Hazard - failure to follow instructions could lead to electrical shock
Safety Precautions
Before replacing the lamp or fuses always turn off the power switch and disconnect the power cord. Always use the correct type and rated fuses. Using incorrect fuses could result in damage to the microscope and electrical shock. Only use the supplied power cord and ensure that you are using a properly grounded outlet.
It is normal for the illuminator housing to get hot during use. Be careful to avoid touching the illuminator housing until the unit has had sufficient time to cool after switching off.
SpecificationsSpecifications
SpecificationsSpecifications
Specifications
26
Viewing Head: Trinocular
Viewing Head Type: Seidentopf
Head Inclination: 30°
Sliding Prism for Trinocular Port: 100/0 Split
Interpupillary Adjustment: 55-75mm
Dioptric Adjustment: -5 to +5
Eyepiece Magnification: 10X High Eyepoint, Ultra-Widefield
Eyepiece Field Diameter: 22mm
Nosepiece: Quintuple, reverse facing
Brightfield Objectives: 5X [0.12 N.A., 26mm W.D.]
(Plan Achromatic, Infinity, LWD) 10X [0.25 N.A., 20mm W.D.]
20X [0.40 N.A., 9mm W.D.]
40X [0.60 N.A., 4mm W.D.]
Stage Dimensions: 140mm x 210mm
Stage Motion: Right-Hand Coaxial Control/Rack & Pinion Drive
Stage Movement Range: 752x 63mm
Focusing Movement: Coaxial Coarse & Fine Controls
Focusing Graduation: 2 microns Per Division
Brightfield Condenser: 0.85 N.A. Abbe Condenser with Iris Diaphragm
Transmitted Illumination: 30W, 12V Variable Quartz Halogen
Reflected Illumination: 50W, 12V Variable Quartz Halogen
Fuse: 3A, 250V
Voltage: 110V [Standard], 220V [Optional]
Base Dimensions: 280mm x 250mm
Overall Dimensions: 470mm (L) x 250mm (W) x 520mm (H)
Weight: 10kg
Page 4
ComponentsComponents
ComponentsComponents
Components
4
Lamp Housing
Lamp Focus Knob
Eyepieces
with
Eyecups
Trinocular Port
Dust Cap
Trinocular Port
Diaphragm Centering Adjustment (left)
Head Set Screw
Prism Selector
Knob
Illumination
Selector Knob
Diopter
Adjustment
Objective
Turret
Mechanical Stage
Substage Control
Fine Focus Control
Coarse
Focus
Control
Focus Tension Control
Power Switch
Illumination
Selector Switch Base
Transmitted Illumination
Aperture Diaphragm
Glass Stage Insert
Transmitted Illumination
Field Diaphragm
TT
TT
T
roubleshootingroubleshooting
roubleshootingroubleshooting
roubleshooting
25
For information about parts, accessories, or service -- contact your dealer directly or contact VanGuard Microscopes at 1-800-423-8842.
Symptom: Can’t focus on specimen image
Step # Possible Cause How To Test Test Result? Solution For More Info
2
1
Dirty objective
Visually inspect
Objective is clean
Objective is not clean
Go to next step
Clean objective or replace with new objective
Page 23
Stage limit set too low
Check position of stage limit by loosening the control and adjusting the focus
Stage limit set correctly
Set too low
Contact dealer or VanGuard Microscopes
Raise stage and set stage limit
Page 15
Symptom: The stage keeps drifting downward
Step #
Possible Cause How To Test Test Result? Solution For More Info
1
Focus tension is set too loose Check focus tension control
Tension too loose
Tension set correctly
Increase tension
Contact dealer or VanGuard Microscopes
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Stand
Symptom: Image through eyepieces is too dim
Step #
Possible Cause How To Test Test Result? Solution For More Info
1
2
Variable lighting control (VLC) is turned down too far
Visually inspect
VLC is turned up
VLC is turned down
Go to next step
Turn VLC up
Page 12, 16
Lamp not centered properly Visually inspect
Lamp is centered
Lamp is not centered
Go to next step
Center the lamp
Page 13, 17
3
Dirty condenser, eyepiece, or objective lenses
Visually inspect
Lenses are dirty
Lenses are clean
Clean condenser, eyepiece, or objective lenses
Contact dealer or VanGuard Microscopes
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Symptom: No image visable in eyepiece and/or trinocular port
Step # Possible Cause How To Test Test Result? Solution For More Info
1
6
4
3
2
Light is not switched on
Visually inspect
Switched on
Not switched on
Go to next step
Move power switch to on position
Page 12, 16
Variable lighting control (VLC) is set too low
Visually inspect
Not set too low
Set too low
Go to next step
Increase VLC level
Objectives not installed or turret is set to empty position
Visually inspect
Objectives are installed
Objectives not installed
Go to next step
Install objectives or change turret position
Page 10
Prism selector knob in wrong position
Visually inspect
In correct position
Not in correct position
Go to next step
Set to correct position Page 11
Light path blocked (transmitted illumination)
Visually inspect space between illuminator and objectives
Blockage present
Nothing blocking
Remove blockage
Contact dealer or VanGuard Microscopes
Page 18
Page 12, 16
5
Illumination selector switch and/or knob are set at incorrect positions
Visually inspect
In correct position
Not in correct position
Go to next step
Set to correct position Page 12, 16
Page 5
ComponentsComponents
ComponentsComponents
Components
5
Trinocular Port
Set Screw
Reflected
Illumination
Analyzer Knob
Reflected
Illumination
Polarizer Slider
Diaphragm
Centering
Adjustment
(right)
X-Axis Lamp
Adjustment
Knob
Lamp
Housing
Door
Y-Axis Lamp
Adjustment
Knob
Lamp Housing
Power Cord
and Plug
Stage Limit
Main Power
Jack
Fuse Holder Variable Lighting Control
Y-Axis Stage
Control
Z-Axis Stage
Control
Drop-In Colored Filter Holder
Swing-In Condenser Lens
Objectives
Head
Reflected Illuminator
Reflected Illuminator Set Screw
TT
TT
T
roubleshootingroubleshooting
roubleshootingroubleshooting
roubleshooting
24
This chart may help resolve some of the more common problems associated with using a compound microscope. Simply follow the steps until your problem is resolved. As always, you can contact your dealer or VanGuard Microscopes if you ever need help.
For information about parts, accessories, or service -- contact your dealer directly or contact VanGuard Microscopes at 1-800-423-8842.
Symptom: No light visable from reflected (upper) illuminator
Step # Possible Cause How To Test Test Result? Solution For More Info
1
7
6
5
4
3
Light is not switched on Visually inspect
Switched on
Not switched on
Go to next step
Move power switch to on position
Page 12
Main power cord and/or illuminator plug not connected
Visually inspect
Plugged in
Not plugged in
Go to next step
Plug in power cord or illuminator plug
Variable lighting control (VLC) turned all the way down
Visually inspect
VLC turned up
VLC turned down
Go to next step
Turn VLC up
Page 16
Microscope not getting power Inspect outlet
Good outlet
Outlet not good
Go to next step
Plug power cord into working outlet
Blown fuse
Visually inspect or try new fuse
Good fuse
Fuse is blown
Go to next step
Install new fuse
Page 22
Burnt out lamp Visually inspect or try new lamp
Good lamp
Lamp is burnt out
Contact dealer or VanGuard Microscopes
Install new lamp Page 21
2
Illumination selector switch is not set to the “UP” position
Visually inspect
Set to “UP”
Set to “DOWN”
Go to next step
Switch the selector to “UP”
Page 12
Page 11
Page 11
Symptom: No light visable from transmitted (lower) illuminator
Step #
Possible Cause How To Test Test Result? Solution For More Info
1
7
6
5
4
3
Light is not switched on Visually inspect
Switched on
Not switched on
Go to next step
Move power switch to on position
Page 16
Main power cord and/or illuminator plug not connected
Visually inspect
Plugged in
Not plugged in
Go to next step
Plug in power cord or illuminator plug
Variable lighting control (VLC) turned all the way down
Visually inspect
VLC turned up
VLC turned down
Go to next step
Turn VLC up
Page 16
Microscope not getting power Inspect outlet
Good outlet
Outlet not good
Go to next step
Plug power cord into working outlet
Blown fuse Visually inspect or try new fuse
Good fuse
Fuse is blown
Go to next step
Install new fuse
Page 22
Burnt out lamp Visually inspect or try new lamp
Good lamp
Lamp is burnt out
Contact dealer or VanGuard Microscopes
Install new lamp
Page 21
2
Illumination selector switch is not set to the “Down” position
Visually inspect
Set to “Down”
Set to “Up”
Go to next step
Switch the selector to “Down”
Page 16
Page 11
Page 11
Brightfield Abbe Condenser
Page 6
ComponentsComponents
ComponentsComponents
Components
6 23
MaintenanceMaintenance
MaintenanceMaintenance
Maintenance
The eyepieces and objectives on this microscopes are coated. They should never be wiped while dry as any dirt or dust will scratch the coating. The surfaces should be blown off and cleaned with an air-bulb and camel-hair brush. It is recommended to then use a lens cleaning solution. Never use anything other than lens cleaning solution on any optical component. Apply with a cotton swab for a minimum of wetting, then wipe the surface clean with a quality lens tissue. These items are all available in our lens cleaning kit, see the Optional Accessories page for ordering information.
The same care instructions apply to all optical components of this microscope, including the illuminator and condenser lenses.
Never dissasemble objectives in an attempt to clean them. The placement and orientation of the internal lenses are absolutely critical to their performance, therefore keeping the exterior surfaces clean to prevent contaminate ingress is essential to the longevity of the objectives.
All other parts can be cleaned with a paper towel and mild detergent. It’s not recommended to use rubbing alcohol for cleaning as it can damage the painted surfaces. Also be aware that rubbing alcohol will break down lubricants, so be careful when cleaning near the following parts:
à Stage rack and pinion gears
à Focus controls
à Objective turret
à Condenser rack gears
à Condenser rack controls
Xylene, since it breaks down the bonding material holding the lenses, should never be used as a cleaner.
Periodically your microscope should be fully serviced by a qualified service technician.
In order to keep dust and debris out of the optical pathways, always keep the camera port and eyetubes covered (with either eyepieces or dust caps), and always use the dust cover when the microscope is not in use.
It’s not recommended to use a compressed gas canister as the liquid propellent can escape and will damage the optical coatings if sprayed onto the lens surfaces.
Caring for your 1442MMi Microscope
Condenser Centering Knobs
Condenser Set Knob
Reflected
Illumination
Polarizer Slider
Polarizer
Rotation Wheel
Interpupilliary Adjustment
Interpupilliary Scale
Page 7
ComponentsComponents
ComponentsComponents
Components
7
MaintenanceMaintenance
MaintenanceMaintenance
Maintenance
22
1. Remove the fuse holder from the rear of the microscope by using a slotted screwdriver to push downward on the fuse comparment door slot while prying outward (figure 60).
2. Remove the fuse from the fuse holder (figure 61).
3. If the fuse is blown replace by inserting a new fuse into the fuse holder.
4. Push the fuse holder back into the fuse holder slot until fully seated.
Replacing the Fuse
Before attempting to replace or remove the fuse, UNPLUG THE MICROSCOPE FROM ANY POWER SOURCE.
!
Replacement Fuse - 3A 250V (Cat. No. 1400-FS5)
If the microscope is plugged in but the illuminator is not turning on, the fuse could be blown. The instructions below detail how to check for a blown fuse. You can usually tell a blown fuse by if the wire inside is broken, or the glass is blackened. Sometimes there will be no visible indication though and it will be necessary to check the fuse with a conductivity meter or to replace it with a new fuse.
For information about parts, accessories, or service -- contact your dealer directly or contact VanGuard Microscopes at 1-800-423-8842.
Figure 60
Figure 61
Colored Filter
Wheel
Field Diaphragm
Lamp Housing
Set Screw
For information about parts, accessories, or service -- contact your dealer directly or contact VanGuard Microscopes at 1-800-423-8842.
Aperture Diaphragm
Page 8
AssemblyAssembly
AssemblyAssembly
Assembly
8
MaintenanceMaintenance
MaintenanceMaintenance
Maintenance
21
1. Remove the glass stage insert and unplug the main power cable and reflected illuminator power cable from the rear of the microscope stand. Carefully lay the microscope onto it’s back.
2. Open the lamp access compartment located on the bottom of the microsope base by turning the set knob (figure 57) counter-clockwise until the door comes loose.
3. When replacing, insert the new lamp into the lamp socket (figure 58). Make sure that the pins on the lamp line up with the holes on the lamp socket. The pins should slide freely into the holes with only slight resistance - do not force.
4. Close the compartment door and retighten the set knob. Follow the instructions in the Transmitted Illumination Setup section to properly center the lamp.
Replacing the Transmitted Illuminator Lamp
Before attempting to replace or remove the lamp, UNPLUG THE MICROSCOPE FROM ANY POWER SOURCE and allow to cool.
!
Be careful not to touch the glass bulb when replacing ­use a tissue or other medium to grasp the lamp. This will prevent the oils in your hand from reducing lamp life. If contact is made with the glass bulb, clean with rubbing alcohol and allow a brief drying period.
!
Replacement Lamp - 30W 12V Halogen (Cat. No. 1400-30W12VHL)
1. Open the lamp housing door located at the rear of the reflected illuminator
box by pulling downward (figure 59).
3. When replacing, insert the new lamp into the lamp socket. Make sure that the pins on the lamp line up with the holes on the lamp socket. The pins should slide freely into the holes with only slight resistance - do not force.
4. Close the compartment door and follow the instructions in the Reflected Illumination Setup section to properly center the lamp.
Replacing the Reflected Illuminator Lamp
Replacement Lamp - 50W 12V Halogen (Cat. No. 1400-50W12VHL)
Figure 57
Figure 59
Contact us for current pricing and part numbers
Camera Kits
USB with Image Capture Software DSLR CCD Video Video Eyepiece
Eyepiece Reticles
5mm Scale 10mm Scale
0.500” Scale 10mm Grid Crosshair Pointer Metric & SAE Scale Calibration Plates
Objectives
50X LWD 0.70NA, 3.68mm Working Distance 60X LWD 0.75NA, 3.18mm Working Distance 80X LWD 0.80NA, 1.25mm Working Distance 100X LWD 0.85NA, 0.40mm Working Distance
Miscellaneous
Microscope Optical Cleaning Kit 4”x6” Lens Paper Optical Tissue
Included Parts
Optional Accessories
Trinocular Head Assembly 1 ea. Reflected Illuminator Assembly 1 ea. Reflected Illuminator Lamp Housing 1 ea. Stand 1 ea. Brightfield Abbe Condenser 1 ea. 10X High Eyepoint Ultra-Widefield Eyepiece 2 ea. 5X Plan Infinity LWD Objective 1 ea. 10X Plan Infinity LWD Objective 1 ea. 20X Plan Infinity LWD Objective 1 ea. 40X Plan Infinity LWD Objective 1 ea. Glass Stage Insert 1 ea. Dispersion Filter 1 ea. Blue Filter 1 ea. Spare 30W/12V Halogen Lamp for Transmitted Illuminator (#1400-30W12VHL) 1 ea. Spare 50W/12V Halogen Lamp for Reflected Illuminator (#1400-50W12VHL) 1 ea. Spare 3A Fuse (#1400-FS5) 1 ea. Power Cord 1 ea. Rubber Eyecups 2 ea. 2mm Hex Wrench 1 ea. 3mm Hex Wrench 2 ea. Operation Manual 1 ea. Warranty Card 1 ea. Dust Cover 1 ea.
For information about parts, accessories, or service -- contact your dealer directly or contact VanGuard Microscopes at 1-800-423-8842.
Figure 58
set knob
lamp socket
lamp
socket
housing
door
For information about parts, accessories, or service -- contact your dealer directly or contact VanGuard Microscopes at 1-800-423-8842.
Page 9
AssemblyAssembly
AssemblyAssembly
Assembly
9
20
Microscope UseMicroscope Use
Microscope UseMicroscope Use
Microscope Use
Figure 52
Figure 53
Figure 54
Figure 55
Figure 56
Colored Filters - Transmitted
6. The transmitted Kohler light illuminator features a drop-in filter holder (figure
52) for use with the included colored absorption filters. These filters can be used for color correction and image enhancement during photomicrography and general use. The filters can be used alone or stacked in order to achieve desired results.
Colored Filters - Reflected
7. The reflected illuminator features a filter wheel (figure 53) containing colored
absorption filter settings, a dispersion filter, and an open setting. As with the transmitted filters they are used for color correction and image enhancement.
Polarizer/Analyzer - Transmitted
8. Simple polarization is possible when utilizing the polarizer function in order to
enhance contrast in certain types of specimens. The transmitted illuminator uses a removable polarizer that can be placed on top of the Kohler light field diaphragm (figure 54). The filter is then rotated to the desired level of extinction.
Polarizer/Analyzer - Reflected
10. As with the transmitted polarizer, the reflected illumination also features a
simple polarization function. Located at the right side of the reflected illuminator is the analyzer knob which must be pushed inward as well as the polarizer slider (figure 56). Polarization is achieved by turning the rotation wheel at the end of the polarizer slider (figure 56) to the desired level of extinction.
9. The analyzer knob, located on the right side of the reflected illuminator, must be pushed inward to engage this function (figure 55).
!
1. Carefully remove all components from foam packaging and plastic bags. Use the diagrams and the parts list in the Components section of this manual to verify that all parts are present. Be sure to carefully check that you’ve removed all the parts from the foam packaging as some are small and can be easily overlooked. Please retain all packaging for future transport.
2. Place the stand on a stable and level work surface (figure 1).
Unpacking Components
Head and Reflected Illuminator Assembly
3. Remove the head from the stand by loosening the reflected illuminator set screw (figure 2) using the included 2mm hex wrench. Grasp the head firmly before loosening the set screw to prevent it from falling. Set
the head aside.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
4. Remove the translucent packing cap from the bottom of the reflected illuminator. Loosen the head set screw (figure 3) and remove the black packing cap from the top of the illuminator. These can be set aside with the packaging as they will not be needed.
5. Insert the dovetail flange located on the bottom of the reflected illuminator into the top of the stand with the black side facing down (figure 4). The end of the illuminator with the round opening should be facing towards the rear of the stand. Use the 2mm hex wrench to tighten the illuminator set screw firmly.
6. Insert the dovetail flange on the lamp housing into the hole located on the rear of the reflected illuminator. Hold in place while tightening the lamp housing set screw (figure 5) with the 2mm hex wrench.
set screw
filter holder
filter wheel
polarizer
analyzer
polarizer
rotation wheel
set
screw
set
screw
packing
cap
packing
cap
Page 10
SetupSetup
SetupSetup
Setup
10 19
1. Focusing adjustment is achieved by turning the coarse/fine focus controls located on both sides of the microscope. The large knob is used for coarse adjustment, the smaller knob for fine adjustment (figure 48). The coaxial arrangement allows for easy, precise adjustment without stage drift.
Focusing and Mechanical Stage Mechanisms
Microscope UseMicroscope Use
Microscope UseMicroscope Use
Microscope Use
Figure 49
Figure 50
Figure 51
3. The mechanical stage X-Y controls, located underneath the right-hand side of the stage (figure 49), provide easy and accurate positioning of the specimen. One complete turn of the longitudinal (Y-axis) control (lower half of the stage controls) will move the specimen 20mm left or right. One complete turn of the transverse (X-axis) control (upper half of the stage controls) will move the specimen 33mm front or back.
2. Turning the coarse/fine focus control raises and lowers the stage vertically. One complete turn of the fine focusing knob raises or lowers the stage
0.2mm; the smallest graduation refers to 2 microns of vertical movement.
One half turn of the coarse focusing knob raises or lowers the stage 20mm.
To ensure long life, turn the focusing knobs slowly and uniformly. Care should always be taken when using the focus controls to prevent the objectives from making contact with the stage or specimen.
Abbe Condenser Swing-In Lens
4. The transmitted illumination Abbe condenser features a swing-in lens located
at the bottom of the condenser (figure 50). This lens should be swung into the light path when using the 5X objective only. This is necessary to obtain the correct Numerical Aperture required for the low powered objective. In addition you will need to fully open the aperture iris in order to prevent vignetting and leave the condenser height the same as when originally setup using the 10X objective.
5. To control contrast in your specimen image while operating with the 5X objective use the field diaphragm (figure 51) instead of the aperture diaphragm that is typically used.
7. Insert the round electrical plug from the lamp housing into the jack located on the rear of the stand (figure 6). Tighten the outer collar to prevent the cable from coming loose.
Head and Reflected Illuminator Assembly (cont.)
Objective Installation
10. Adjust the coarse focus knobs so that the stage is completely lowered.
11. Remove the objectives from their packing canisters and thread each one
into the nosepiece holes ensuring they are fully seated (figure 9). Typically they are installed in numerical order from lowest to highest magnification.
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
8. Insert the dovetail flange located on the bottom of the head into the hole at the top of the reflected illuminator. The eyetubes should be facing forward. While still holding the head tighten the head setscrew with the 2mm hex wrench (figure 7).
9. Remove the two packing caps from the eyetube openings and set aside with the other packaging as they will not be needed. Slide the eyepieces into the eyetubes and ensure they are fully seated. If you wish you may attach the eyecups to the ends of the eyepieces, though this step is optional and eyeglass wearers especially may choose not to install the them (figure 8).
Stage Plate Installation
12. With the stage completely lowered place the glass stage plate into the
rectangular recess on top of the stage (figure 10). Clean the plate first if necessary.
collar
eyepieces
eyecups
objectives
stage plate
Figure 48
right side
focus
controls
left side
focus
controls
y-axis
x-axis
swing-out lens
field diaphragm
Page 11
SetupSetup
SetupSetup
Setup
1118
SetupSetup
SetupSetup
Setup
36. Open the field diaphragm until the iris is just barely no longer visible (figure 45).
37. To eliminate stray light glare close the aperture iris until you have achieved acceptable contrast in your specimen.
Figure 45
Figure 46
Figure 47
Kohler Illumination Setup (continued)
Trinocular Camera Port Setup
Installation and setup of your camera system, if applicable, will vary depending on the camera type and adapters that are utilized. Please refer to the instructions that came with your camera adapter system for specific details.
38. In general though, most systems will require the removal of the trinocular port dust cap which is held in place by a set screw located at the rear of the port (figure 46).
39. Use the prism selector knob, located on the left side of the microscope head (figure 47), to divert the image from the eyepieces to the camera. The system uses a 100/0 split sliding prism to divert 100% of the light to the camera port when selected. This provides the brightest image possible to the camera which can be critical for certain photomicrographic applications.
This concludes the setup of your VanGuard 1442MMi industrial microscope. At this point your microscope is ready for use. Continue for instructions on the proper use of the instruments main components.
Abbe Condenser Installation
Your microscope will have come with the brightfield condenser installed but should you need to reinstall at any point follow the directions below.
13. Rotate the nosepiece so that either the 5X objective or an empty slot is in the light path.
14. Using the coarse focus knobs raise the stage completely. Be careful to not crash the stage into the objectives. If the stage won’t raise fully check to see if the stage limit is set too low. Refer to the Stage Limit instructions in the Setup section of this manual for more information.
15. Adjust the substage control so that the substage assembly is in the full downward position (figure 11).
16. Slip the brightfield condenser into the collar from below. The narrow end of the condenser should be facing upward. With the iris control facing forward, and centered properly, tighten the condenser set knob (figure 12).
This concludes the basic assembly of your VanGuard 1442MMi industrial mi­croscope. Continue on to setup the optical alignment, illumination, and ergo­nomic features for proper use.
Power Cord Installation
17. Check to see that the main power switch is in the off (“0”) position
(figure 13).
Figure 11
Figure 12
Figure 13
Figure 14
18. Connect the main power cord to the jack located at the rear of the microscope stand (figure 14) and plug the other end into a grounded power source.
19. The microscope electrical system will accept either a 110V/60Hz or a 220V/ 50Hz AC voltage source. If you ordered a 110V microscope it will have come with a 3-Pin North American (NEMA 5) style power cord. A grounded Schuko (CEE 7/7) style cord is included with the 220V microscopes.
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30. Lean the microscope back to expose the lamp adjustment control located on the bottom of the microscope stand taking care not to let the glass stage plate and/or speciments fall. Loosen the transmitted lamp adjustment knob located on the lamp compartment door by turning counter clockwise (figure 40).
Figure 40
Figure 41
Figure 43
Figure 44
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Kohler Illumination Setup
When using the transmitted illuminator proper condenser alignment, iris settings, and focusing are necessary to obtain true Kohler illumination and achieve the highest quality image of your specimen.
33. Open the transmitted illuminator aperture and field diaphragms fully (figure
42), rotate the objective turret so that the 10X objective is in the optical path, and focus on a specimen.
Transmitted Illumination Setup (continued)
34. While viewing through the microscope and slowly closing the field diaphragm,
adjust the substage condenser vertical control until the edge of the field iris is in focus (figure 43).
35. The two control knobs can be turned in unison or independantly to position the condenser in alignment with the field diaphragm and light path (figure
44).
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Reflected Illumination Setup
1. Set the main power switch to the ON position (“|”) and the illuminator selector
switch to the UP position (“||”) (figure 15).
The reflected illuminator housing will get hot if left on for extended periods of time. Be careful when making adjustments to the illuminator housing or lamp fixture.
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3. Ensure that the illumination selector knob is pushed all the way inward (“R”) (figure 17).
Figure 15
Figure 17
Figure 18
Figure 19
4. Rotate the Aperture and Field diaphragm dials to the right (figure 18) to fully
open the iris’s.
5. Rotate the color filter wheel until the blank position is in the light path
(figure 18).
6. Pull the Polarizer filter slider out until you feel a click. Pull the Analyzer selector
knob fully out (figure 18).
7. Rotate the 5X objective into the light path. You may have to lower the stage
in order not to crash the higher magnification objectives into the stage.
8. Look through the eyepieces and you should see a white field of view. You may have to adjust the illuminator intensity dial in case the light is too dim or too bright. If the brightest portion of the field of view isn’t centered (figure 19) you will need to perform the following instructions.
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31. Move the knob in latitudinal and/or longitudinal directions (figure 40) until the field of view as seen through the eyepieces is at it’s brightest overall and the brightest portion is well centered in the field of view (figure 41). This step may be aided by placing a bright white piece of paper on the stage directly under the objective and the light field viewed through that.
32. Retighten the lamp adjustment knob by turning clockwise.
Figure 42
field iris
aperture
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2. Adjust the VLC (variable lighting control) (figure 16) counter-clockwise to the maximum position.
Figure 16
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Figure 36
Figure 37
Figure 38
Figure 39
Transmitted Illumination Setup
23. Set the main power switch to the ON position (“|”) and the illuminator selection
switch to the DOWN position (“|”) (figure 35). Adjust the VLC (variable lighting control), located on the right side of the microscope base, counter-clockwise to the maximum position.
24. Check to see that the illuminator selector knob is pulled out all the way (“T”) (figure 36).
25. Rotate the field diaphragm dial clock-wise to fully open the iris (figure 37).
26. Shift the aperture diaphragm slider to the left to fully open the iris (figure 37).
27. Remove the transmitted polarizer filter from the collector if applicable. Pull
the Analyzer selector knob fully out (figure 38).
28. Rotate the 5X objective into the light path. You may have to lower the stage in order not to crash the higher magnification objectives into the stage.
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9. Adjust the three reflected illuminator alignment knobs located on the illuminator housing in order to properly align the lamp. The knob on the left (figure 20) focuses the lamp transversely (Z-axis) while the upper right knob adjusts for latitudinal (X-axis) and the lower for longitudinal (Y-axis) movement (figure 21).
Figure 20
Figure 21
Figure 22
Figure 23
Figure 24
10. Rotate each of these in succession until the field of view is at it’s brightest overall and the brightest portion is centered in the field of view (figure 22). This step may be aided by placing a bright white piece of paper on the stage directly under the objective and the light field viewed on that.
Reflected Illumination Setup (continued)
11. Next, the reflected illuminator field diaphragm needs to be properly centered
in the light path. Ensure that the reflected illuminator aperture diaphragm is fully open by rotating the Aperture dial to the right (figure 23).
Reflected Field Diaphragm Alignment
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12. While looking through the eyepieces close the field diaphragm by rotating the Field dial to the left until you begin to see a darker perimeter in the field of view resembling an iris silhouette(figure 24).
Figure 35
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power
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29. Look through the eyepieces and you should see a white field of view. You may have to adjust the variable lighting control in case the light is too dim or too bright. If the brightest portion of the field of view isn’t centered (figure 39) you will need to perform the following instructions.
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13. If it is not well focused use the main focus controls to bring it in so. Set the iris opening to where it almost completely fills the field of view (figure 25).
Reflected Field Diaphragm Alignment (continued)
Figure 25
Figure 26
Figure 27
Figure 28
Figure 29
14. If the iris is not properly centered in the field then use the two included 3mm hex wrenches to center it. Place one into each of the access holes located on either side of the reflected illuminator (figure 26). Make sure they completely engage the hex screws located within.
15. While looking at the field diaphragm silhouette through the eyepieces, rotate the two hex wrenches back and/or forth until the diaphragm is properly centered (figure 27). You can make adjustments to the diaphragm using the Field dial in order to aid in this process.
Eyecups (optional)
16. The rubber eyecups can be mounted to the eyepieces (figure 28) in order to
prevent stray light from entering and therefore providing a higher contrast image. Eyeglass wearers who choose to wear their glasses during microscope use should remove the eyecups in order to maintain proper eyepoint positioning to the eyepiece. You will want to be careful not to make contact between the eyepiece lenses and your eyeglasses to protect the delicate optical coatings though.
Interpupilliary Adjustment
17. Place a specimen on the stage and while looking through the eyepieces fold
the eyetubes together or further apart (figure 29) to make the two images converge into one field of view. This adjustment will be different for all users and should be checked and readjusted before each use.
Focus Adjustment
18. Focusing on your specimen is achieved through rotating the coarse and/or
fine focusing knobs. These are located on either side of the stand in a coaxial layout (figure 30). The outer-most dials control the fine focus and the larger dials located just inward control the coarse focus adjustments.
19. The inner-most dial located on the left focus adjustment (figure 31) controls the stage movement tension. This should be adjusted to where the focus controls are smooth and easy to turn but not so loose that the stage drifts out of focus.
Focus Tension Adjustment
Figure 30
Figure 31
Figure 32
Figure 33
Figure 34
Stage Limit
20. Located inward of the right hand coarse focus adjustment (figure 32) is the
stage limit which prevents the stage from crashing into the objectives.
Diopter Adjustment
22. Before each use of the microscope it’s important to check and readjust the
diopter if necessary. With the main focus controls, and viewing only with your right eye, bring the specimen into focus. Then view the image using only your left eye and rotate the diopter ring located around the left eyetube (figure 34) to bring the image into focus.
21. To adjust, first unlock the limit by rotating it counter-clockwise (figure 33). With the highest power objective in the light path adjust the stage until the image through the eyepieces is in focus then lock the limit by rotating the control clockwise until tight (figure 33). The upward motion of the stage when using the coarse focus controls is now limited to this point. The fine focus controls are not affected though and will still allow upward movement of the stage.
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