Valeport Hyperion Operating Manual
Hyperion Fluorometer
Operating Manual
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reprinted or reproduced in any material form, either wholly or in part, and the contents of this document, and
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the prior written consent of the Company.
Valeport Limited
St Peters Quay
Totnes
Devon, TQ9 5EW
United Kingdom
As part of our policy of continuous development, we reserve the right to alter, without prior notice, all
specifications, designs, prices and conditions of supply for all our equipment.
0901814c
Monday, October 15, 2018
+44 1803 869292
sales@valeport.co.uk | support@valport.co.uk
www.valeport.co.uk
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e mail:
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Table of Contents
© 2018 Valeport Ltd
Page 2
Table of Contents
..................................................................................................................................... 31. EU Declaration of Conformity - CE Marking
..................................................................................................................................... 52. Introduction - Hyperion Fluorometer
.................................................................................................................................... 52.1. Fluorophore & Nomenclature
..................................................................................................................................... 63. Sensors
.................................................................................................................................... 63.1. Fluorometers
3.1.1 Safety Statement
.................................................................................................................................... 6
3.1.2 Chlorophyll a
.................................................................................................................................... 6
3.1.3 Fluorescein
.................................................................................................................................... 7
3.1.4 Rhodamine
.................................................................................................................................... 7
.................................................................................................................................... 83.2. Linear Observation Range
.................................................................................................................................... 93.3. Quenching
.................................................................................................................................... 103.4. Turbidity
3.4.1 Safety Statement
.................................................................................................................................... 10
..................................................................................................................................... 114. Data Acquisition
.................................................................................................................................... 114.1. Setting the Gain
.................................................................................................................................... 134.2. Output Rate
.................................................................................................................................... 134.3. Functional Check
..................................................................................................................................... 175. Operation with MIDAS CTD+
..................................................................................................................................... 186. Electrical
.................................................................................................................................... 186.1. Connector Pin-Out
..................................................................................................................................... 197. Communications
.................................................................................................................................... 197.1. Data Output Formats
.................................................................................................................................... 207.2. Operating Modes
7.2.1 Mode C - Continuous
.................................................................................................................................... 20
7.2.2 Mode M - Continuous Measurement
.................................................................................................................................... 20
.................................................................................................................................... 217.3. Hash (#) Codes
..................................................................................................................................... 228. Physical Characteristics
.................................................................................................................................... 228.1. Dimensions
..................................................................................................................................... 239. Care and Maintenance
..................................................................................................................................... 2410. Software - DataLog x2
..................................................................................................................................... 2511. Ordering and Part Numbers
© 2018 Valeport Ltd
EU Declaration of Conformity - CE Marking
Page 3
1. EU Declaration of Conformity - CE Marking
© 2018 Valeport Ltd
0901814c - Hyperion Fluorometer
Page 4
Please note: Any changes or modifications to the product or accessories supplied, that are not
authorised by Valeport Ltd, could void the CE compliance of the product and negate your authority
to operate it. This product has demonstrated CE compliance under conditions that include the use of
shielded cables. It is important that you use shielded cables compliant with the product’s
conformance, to protect from potential damage and reduce the possibility of interference to other
electronic devices.
© 2018 Valeport Ltd
Introduction - Hyperion Fluorometer
Page 5
2. Introduction - Hyperion Fluorometer
Valeport’s Hyperion Fluorometer sensor range delivers high performance measurements of
Chlorophyll a or Fluorescein in a compact & robust package ideal as a standalone sensor, for ROV
and AUV integration or used as part of a multi-sensor array and data logger.
Offered as standard in a 6000m depth rated, Titanium housing the Hyperion Fluorometer has a wide
range (9-30V DC) isolated power supply, data output up to 32Hz and RS232 communications.
The Hyperion is an accurate single channel detector which can be used for many different
fluorophores. It is designed for integration into systems providing electrical power and delivers a
signal that has been correlated to a known concentration of fluorophore.
2.1. Fluorophore & Nomenclature
“C”
Chlorophyll
“F”
Fluorescein
"R"
Rhodamine
More Fluorophores will become available in the Hyperion family of products - please check with
Valeport for availability.
Other optical, non-fluorescent technology:
“T”
Turbidity
© 2018 Valeport Ltd
0901814c - Hyperion Fluorometer
Page 6
3. Sensors
An optical sensor must be kept clean to operate correctly. Ensure that the SWiFT is power down
before cleaning the sensor.
Use warm soapy water with a soft bristled brush to remove any light fouling
For heavy fouling use a solvent (e.g Isopropyl alcohol) and a soft bristled brush
Always rinse thoroughly after every use in clean, fresh water.
3.1. Fluorometers
3.1.1. Safety Statement
A Hyperion Fluorometer is classified as Risk Group 1 under standard 62471. As the type is classified as
Risk Group 1 solely due to radiation in the visible band a hazard label is not required. However,
· the LED used is in excess of the Exempt Group and that the viewer- related risk is dependent upon
how the user installs and operates the equipment.
· the exposure hazard value (EHV) for a Hyperion Fluorometer in terms of distance is 320mm
Never look directly into the Hyperion aperture
3.1.2. Chlorophyll a
Performance
Excitation:
470 nm
Detection:
696 nm
Dynamic Range:
0-800 µg/l
2 gain settings: 0-40 and 0-800 (software controlled)
Instrument
Detection limit:
0.025 µg/l*
Actual
Detection limit:
0.025 µg /l**
Linearity:
0.99 R
2
Response Time:
0.03 to 2 sec
* 3x SD in RO water
** calibrated against Chlorophyll a in acetone solution
© 2018 Valeport Ltd
Sensors
Page 7
3.1.3. Fluorescein
Performance
Excitation:
470 nm
Detection:
545 nm
Dynamic Range:
0-500 ppb
2 gain settings: 0-25 and 0-500 (software controlled)
Instrument
Detection limit:
<0.01 ppb*
Actual
Detection limit:
0.03 ppb**
Linearity:
0.99 R
2
Response Time:
0.03 to 2 sec
* 3x SD in RO water
** Calibrated against Fluorescein solution
3.1.4. Rhodamine
Performance
Excitation:
520 nm
Detection:
650 nm
Dynamic Range:
0-1000 ppb
2 gain settings, 0-50, 0-1000 (software controlled)
Instrument
Detection limit:
<0.01 ppb*
Actual
Detection limit:
0.06 ppb**
Linearity:
0.99 R
2
Response Time:
0.03 to 2 s
* 3x SD in RO water
** Calibrated against Rhodamine solution
© 2018 Valeport Ltd
0901814c - Hyperion Fluorometer
Page 8
3.2. Linear Observation Range
The linear range is the concentration range for which the fluorometer signal is directly proportional to
the concentration of the fluorophore. The linear range starts at the minimum detection limit (MDL)
and extends to the upper limit of the instrument (dependent on fluorophore properties, optical filters,
LED power, sample volume and optical path length).
Hyperion Fluorometers have a calibrated linear response for 2 gain settings (e.g. the ranges 0-40 µg/l
(G5) and 0-800 µg/l (G1) for chlorophyll a). At higher concentrations, unlike analogue devices which
generally flat-line at full-scale deflection (e.g. FSD 5V) the Hyperion will continue to output a signal
which increases with concentration (i.e. meaningful data), though which is no longer guaranteed to
be linear.
At very high fluorophore concentrations, signal quenching can occur, whereby the instrument output
does not increase linearly with fluorophore concentration (roll-off) and may decrease at even higher
levels.
To perform a quick linearity check, dilute the sample 1:1 with RO water. If the reading decreases by
50%, the sample is in the linear range. If the reading decreases by less than 50% or even increases,
the sample is above the linear range.
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