TURNER DESIGNS C-FLUOR User Manual

Digital

User’s Manual

March 13, 2020
P/N 998-2131
Revision 1.0

TURNER DESIGNS
1995 N. 1st Street
San Jose, CA 95112
Phone: [phone]
FAX: [phone]

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 2

Table of Contents

1.

Introduction

1.1

Description

4

2.

Inspection and Setup

2.1

Instrument Checklist

5

2.2

Configurations

5 2.3

Optional Accessories

5

2.4

Functional Test for Digital C-FLUOR with connector

7

2.5

Digital C-FLUOR with no connector

8

3.

Digital C-FLUOR Software & Functions

3.1

Setup

9

3.1.1

Polling Mode

9

3.1.2

Data Streaming Mode

10

3.13

Setting New Baud Rate

10

3.2

Calibration

11

3.2.1

Calibrating the Digital C-FLUOR Probe

11

3.2.2

Restoring Factory Calibration

12

4.

Maintenance and Warranty

4.1

Maintenance

13

4.1.1

Rinsing

13

4.1.2

Care for the bulkhead connector

13

4.1.3

Care for the Optics

13

4.2

Warranty Terms

13

4.3

Warranty Service

14

4.4

Out of Warranty Service

14

Appendices

A Specifications

16

B Recommended Lab Measurement Practices

17 C

Wiring Guide

18

D Pigtail Cable and Connector Information

19

E Digital C-FLUOR Probe Command List

20

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 3

WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE)
DIRECTIVE

Turner Designs is in the business of designing and selling products that benefit the
well-being of our environment. Accordingly, we are concerned with preserving the
surroundings wherever our instruments are used and happy to work with customers
by complying with the WEEE Directive to reduce the environmental impact resulting
from the use of our products.

WEEE Return Process:

To arrange the return of an end-of-life product, proceed as follows:

If you purchased your instrument through a Turner Designs Distributor please
contact your local representative. They will instruct you where to return the end­of-life product.

If you purchased your instrument directly from Turner Designs please contact
Turner Designs Customer Service
By Phone: [phone] or Toll Free: [phone]
By Email: Customer Service at [e-mail]
Turner Designs will provide a WEEE RMA Number, a Shipping Account
Number, and a Ship to Address. Package and ship the product back to Turner
Designs.

The product will be dealt with per Turner Designs’ end-of-life recycling program in
an environmentally friendly way.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 4

1. Introduction

1.1 Description
The Turner Designs Digital C-FLUOR Submersible Probe is an accurate single-

channel detector that can be used for many different applications. Digital C­FLUOR can be integrated into multi-parameter systems from which it receives
power allowing it to output digital ASCII data as concentration estimates for a
fluorophore of interest.

Digital C-FLUOR Probes are factory calibrated and a probe-specific calibration
certificate is included with each probe. The certificate contains information that

states the probe’s minimum level of detection and range of concentrations that

can be measured for the specific application.
Digital C-FLUOR Probes are manufactured with a Titanium housing and

connector, ideal for long-term deployments and able to withstand most corrosive
environments. It is available without a connector for integration flexibility; see
Section 2.2 for part numbers.

NOTE: Digital C-FLUOR Probes cannot be used with the C6 Multi-Sensor
Platform, Cyclops Explorer or Databank Handheld Datalogger.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 5

2. Inspection and Setup

2.1 Instrument Checklist
The Digital C-FLUOR Submersible Probe shipment package consists of:

 A Digital C-FLUOR Submersible Probe that is configured and factory

calibrated for the specified fluorophore or material as noted by the
Identification Letter stamped on the connector:

 “C” = Chlorophyll
 “R” = Rhodamine
 “F” = Fluorescein
 “P” = Phycocyanin
 “E” = Phycoerythrin
 “U” = CDOM / FDOM
 “O” = Crude Oil
 “B” = Optical Brighteners
 “T” = Turbidity

 “D” = Red Excitation Chlorophyll
 Calibration Certificate
 Quick Start Guide

2.2 Configurations

 C-FLUOR (Digital) P/N 2120-000-“Identification Letter”-232
 C-FLUOR Without Connector (Digital) P/N 2120-000-“Identification Letter”-

NC-232

Note: No end cap is supplied for the C-FLUOR Without Connector (Digital) - see
Section 2.5. For end cap specifications, contact [e-mail]

2.3 Optional Accessories
 RS-232 Programming Kit P/N 2120-900 includes RS-232 Programming

cable P/N 2120-160 and 12-volt power supply P/N 7000-941

 Pigtail Cables with Locking Sleeve - see Appendix D for more information

 0.6 Meter Pigtail Cable with Locking Sleeve P/N 2100-750
 5 Meter Pigtail Cable with Locking Sleeve P/N 2100-755
 10 Meter Pigtail Cable with Locking Sleeve P/N 2100-751

 Pigtail Cables longer than 10 meters will require RS422 converter

 25 Meter Pigtail Cable with Locking Sleeve P/N 2100-752

Note: Pigtail Cables should NOT be used to tow instruments.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 6

 Flowthrough Cap P/N 2100-600
 Shade Cap P/N 2100-701

Note: We recommend use of the shade cap as it provides a fixed distance
for sample measurement and minimizes effects from ambient light.

 Solid Secondary Standard (SSS) for in vivo Chlorophyll, Phycocyanin,

Phycoerythrin, Rhodamine, Fluorescein P/N 2100-900

Note: The SSS can be used for both C-FLUOR and Cyclops for the
applications specified above.

 Solid Secondary Standard (SSS) for UV - CDOM / FDOM, Optical

Brighteners, and Crude Oil P/N 2100-904

Note: The UV SSS can be used for both C-FLUOR and Cyclops for the
applications specified above, as well as Cyclops Refined Fuels and
Tryptophan sensors.

Flow Cap

Shade Cap

Solid Secondary Standard

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 7

2.4 Functional Test for Digital C-FLUOR with connector
To perform a functional check on a Digital C-FLUOR Probe, connect the probe

to your computer following the instructions below, using the RS-232
Programming Cable as shown in Figure 1:

Additional Equipment required for functional tests:

RS-232 Programming Cable P/N 2120-160
12-volt power supply P/N 7000-941
Computer with USB port

Note: Supply voltages greater than 15 VDC will damage the probe.

1) Insert the Programming Cable’s USB plug into an available USB port on your

computer and wait for the drivers to automatically install.

2) Once USB drivers have installed, download and install Digital C-FLUOR’s

Software from www.turnerdesigns.com.

3) After software has successfully installed, double-click the software’s icon

which should have been automatically added to your desktop.

4) When software has opened, supply power to the instrument using the 12-volt

power supply specified ensuring the power supply is plugged into an AC
source.

5) Click “Communicate With C-FLUOR” and wait a few minutes for the software

to detect the connected probe.

6) When connected, C-FLUOR Communication Status radio button will change

from red to green.

Figure 1

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 8

With the C-FLUOR connected to your computer and power supply, and
communicating with the software, answer questions 1-3 by making the following
functional tests:

1. Is the LED on?

Hold a piece of white paper about ½ an inch in front of the optical head to
ensure the LED is ON.

Note: This test does not work for Turbidity probes because they use
infrared which is not visible.

2. Is there output?

Click the “Get Datapoint” button; if a value is displayed in the box then the
instrument’s output is working.

3. Does the output change?

Move the light source closer to the piece of paper and again click the “Get
Datapoint” button; if the output increases then the instrument’s detector is
working.

2.5 Digital C-FLUOR with no connector

Digital C-FLUOR can be purchased without a 6-pin bulkhead connector or end
cap. If the No Connector version is purchased the Digital C-FLUOR Probe will
have bare wires as shown in the figure below. See Section 2.1 for identification
letters.

C-FLUOR Without Connector (Digital) P/N: 2120-000-“Identification Letter”-NC-232
Use the wiring guide in Appendix C to wire the Digital C-FLUOR Probe for serial

communication with your computer or controller. Use the command list in
Appendix E to configure your Digital C-FLUOR probe for your data logger or
third-party instrument that accepts an ASCII data string output.

Note: The Digital C-FLUOR Probe is factory calibrated; to re-calibrate the
probe you must connect to a computer via serial connection and use
Digital C-FLUOR Probe software. See Section 3.2 for Calibration.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 9

3. Digital C-FLUOR Software & Functions

Software is used to configure Digital C-FLUOR Probes for digital integration and/or
deployment. Digital C-FLUOR Probes do not store data internally, ASCII data
strings are sent out via serial communication.

3.1 Setup
The Setup tab displays the probe’s serial number and the probe-specific

application; these values cannot be edited. There are two modes that can be
selected, Polling and Data Streaming.

3.1.1 Polling Mode

Polling Mode is used when data storage or power is limited. Power is
supplied to the probe and a command is used to trigger the probe to
make a measurement. See Appendix E for command list. Every poll
command will return an individual ASCII data string that contains a
concentration value relative to the fluorescence detected. To activate
polling mode, simply put a checkmark into the box next to the “Polling”
function and select QUIT to exit the software. Once power is supplied to
the probe, it will be ready to receive a poll command after 0.5 seconds.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 10

3.1.2 Data Streaming Mode

Data streaming mode provides a continuous ASCII digital output string at
selectable data rates. Rates can be accessed using the pull-down menu

located next to the “Data Streaming” box. To activate Data Streaming

mode you must first choose a Rate and then put a checkmark in the box

next to the “Data Streaming” function. The Data Streaming radio button

will change from red to green indicating the unit is streaming data.

Note: Data rates faster than 1 reading per second will be updated at
a rate of 1 reading per second in the Datapoint Value box. To see
the actual data rate users should run a terminal emulator such as
Hyperterminal to view actual data rate set.

3.1.3 Setting a New Baud Rate

Users can change the Baud Rate by clicking the “Set New Baud Rate”
box which will open a window giving the option to select any of five
available baud rates. Place a checkmark in the box next to the desired

baud rate and click “Set New Rate”. The “Current Baud Rate” box will

display the selected Baud Rate.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 11

3.2 Calibration
The Digital C-FLUOR Probe is factory calibrated and the calibration values are

stored in memory. The probe can be re-calibrated by users via software if site­specific calibrations are desired for increased accuracy. New calibration values
will be stored in memory without overwriting or erasing factory calibration values.
This allows users to restore factory calibration values if desired.

Note: Restoring factory calibration values will erase all customer
calibration values.

To calibrate Digital C-FLUOR you’ll need the following:

- PC with Digital C-FLUOR Software

- RS-232 Programming Cable P/N 2120-160

- 12-Volt Power Supply P/N 7000-941

- Blank solution

- Standard solution of known concentration

- Blackened or dark 500 ml beaker/container

3.2.1 Calibrating the Digital C-FLUOR Probe

1) See section 2.4 Functional Test for Digital C-FLUOR with connector
on how to connect to your computer.

2) Click the “Calibration” tab.

3) Enter the known concentration of your Standard Solution in the
Concentration Value box. Note: Only values greater than 1 are
valid.

4) Fill your 500 ml beaker with blank solution.

5) Submerge the probe’s optical head no more than an inch below the
surface of the blank solution. See Appendix B for recommended lab
practices.

SET FIRST

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 12

6) Click the “Calibrate C-FLUOR” button, then click “OK”.

7) Wait for the progress bar to complete.

8) When prompted to insert C-FLUOR into Calibration Solution, remove
the probe from the blank solution.

9) Empty the blank solution from the beaker and wipe the beaker dry.

10) Fill your 500 ml beaker with standard solution.

11) Submerge the probes optical head no more than an inch below the
surface of the standard solution. See Appendix B for recommended
lab practices.

12) Click “OK”.

13) Wait for the progress bar to complete.

14) Click “Yes” to save the new calibration.

15) Click “Get Datapoint” to read the standard solution with the newly
calibrated probe; you should see the Concentration Value you
entered displayed in the “Datapoint Value” box confirming the
calibration was saved.

3.2.2 Restoring Factory Calibration
To restore the factory calibration of your Digital C-FLUOR Probe, you’ll

need to connect your probe to a computer with Digital C-FLUOR
software, communicate with your probe using the software and click on

the Calibration tab. Then click “Restore Factory Calibration”. You will be

asked if you wish to continue, select “Yes” to restore or “No” to keep your

custom calibration.
If factory calibration is restored, the software will display Factory

Calibration was restored meaning your custom calibration was deleted
and cannot be retrieved.

Note: Once customer calibrations are deleted, they cannot be
retrieved. Please be sure you intend to delete your custom
calibration before choosing to restore to factory calibration.

Will be

displayed
after
restoring
factory

calibration

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 13

4. Maintenance and Warranty

4.1 Maintenance

4.1.1 Rinsing
The C-FLUOR should be rinsed or soaked in fresh water following each

deployment until it is completely clean again.

4.1.2 Care for the bulkhead connector
A light coat of Silicone spray should be used on the rubber of the male

pins of the bulkhead to aid in sealing. The manufacturer recommends
3M™ Silicone Lubricant Spray or Loctite 8021 spray.

Note: You should avoid using silicone grease. Do NOT use WD-40,
it will destroy the connectors.

4.1.3 Care for the optics
The optical window should be visually inspected after each deployment

following a soaking in fresh water. If cleaning is needed, use optical
tissue to clean the window with soapy water.

Note: The C-FLUOR should NOT come in contact with any organic
solvents (i.e. acetone, methanol) or strong acids and bases.

4.2 Warranty Terms
Turner Designs warrants the C-FLUOR and accessories to be free from defects

in materials and workmanship under normal use and service for a period of 12
months from the date of shipment from Turner Designs with the following
restrictions:

 Turner Designs is not responsible for replacing parts damaged by accident

or neglect. Your instrument must be installed according to instructions in the
User’s Manual. Damage from corrosion is not covered. Damage caused by
customer modification of the instrument is not covered.

 This warranty covers only Turner Designs products and is not extended to

equipment used with our products. We are not responsible for incidental or
consequential damages, except in those states where this limitation is not
allowed. This warranty gives you specific legal rights and you may have
other rights which vary from state to state.

 Damage incurred in shipping is not covered.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 14

4.3 Warranty Service
To obtain service during the warranty period, the owner shall take the following

steps:

1. Write, email or call Turner Designs Technical Support and describe as

precisely as possible the nature of the problem.

Phone: [phone]
Email: [e-mail]

2. Carry out any adjustments or tests as suggested by Technical Support.

3. If proper performance is not obtained you will be issued a Return Materials

Authorization number (RMA) to reference. Package the unit, write the RMA
number on the outside of the shipping carton, and ship the instrument,
prepaid, to Turner Designs. If the failure is covered under the warranty
terms the instrument will be repaired and returned free of charge, for all
customers in the contiguous continental United States.

For customers outside of the contiguous continental United States who
purchased equipment from one of our authorized distributors, contact the
distributor. If you purchased directly, contact us. We will repair the
instrument at no charge. Customer pays for shipping, duties, and
documentation to Turner Designs. Turner Designs pays for return shipment.
Custom duties, taxes and fees are the responsibility of the customer.

4.4 Out-of-Warranty Service
Follow steps for Warranty Service as listed above. If Technical Support can

assist you by phone or correspondence, we will be glad to, at no charge. Repair
service will be billed on a fixed price basis, plus any applicable duties and/or
taxes. Shipment to Turner Designs should be prepaid. Your bill will include
return shipment freight charges.

Address for Shipment:

Turner Designs, Inc.

1995 N. 1st Street

San Jose, CA 95112

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 15

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 16

Appendix A: Specifications

Parameter

Specification

Full Range Linearity

0.99 R2

Power Draw @ 12V

CHL, RWT, PC, PE, F, CDOM/fDOM, Oil, & OB = 22mA
Turbidity = 20mA

Input Voltage

3 – 15 VDC

Signal Output

ASCII Digital Data String (RS-232)

Temperature Range

Ambient: 0 to 50 C
Water Temp: -2 to +50 C

Light Source

Light Emitting Diode

Excitation Wavelength

Visible – CHL, RWT, PC, PE, F
UV – CDOM/fDOM, Oil, OB
IR – Turbidity

Detector

Photodiode

Detection Wavelengths

400 – 850 nm

T99

< 0.6 seconds

Housing Material

Titanium

Dimensions

C-FLUOR
L: 5.2 in., 13.24 cm
D: 0.875 in., 2.23 cm

No Connector C-FLUOR
L: 3.8 in., 9.68 cm
D: 0.875 in., 2.23 cm

Depth Rating

2,000 meters

Weight

3.3 oz; 95 gm

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 17

Appendix B: Recommended Lab Measurement Practices

Recommended Lab Practices for Measurements

The following steps will improve the accuracy and repeatability of your measurements,
especially at low concentration levels:

1. Use a non-fluorescent container for your water samples. Note: Plastic may
fluoresce and interfere with the sample’s fluorescence.

2. If using a glass container, place the container on a non-reflective black surface.

3. Ensure that the probe is more than 3 inches above the bottom of the container.

4. Ensure that the probe is in the center of the container and has more than 2 inches
clearance between the cirumference of the probe and the inside surface of the
beaker.

>3 inches

Dark/Black
Non-Reflective
Surface

>2 inches all

round

Calibrated

Sensor

No Air Bubbles

On Optical

Surface

Glass Beaker

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 18

Appendix C: Wiring Guide

C-FLUOR

Pins/Wires

Pin

Number

Function

Connection

Red

1

Supply Voltage

3 – 15 VDC

PSU – Positive Connection

Black 2 Supply Ground, 0VDC

PSU – Ground Connection

Orange 3 Receive Data

RX

Green 4 Transmit Data

TX

Blue 5 Digital Ground

GND

Yellow

6

N/A

-

Note: Pin 6 is not used.

Pigtail Connector

and Wire Color

3

6

4

2

1

5

5

2

4

4 6 1

3

C-FLUOR Pins
and Wire Color

3 4 2 1 5 6 6

3

6

4

2

1

5

5

2

4

4 6 1

3

3 3

6 6

4 4

2 2

1

5 5

5

2

4

4 6 1 3 3 4 2 1 5 6 6

3 4 2 1 5 6 3

3

4 4

2 2 1 5 5

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 19

Appendix D: Pigtail Cable and Connector Information

Dimension details of 24” length cable with 20 gauge colored lead wire, connects to 6 pin
male connector. (Cable manufacturer/Part No: IMPULSE/MCIL-6-FS)

Female locking sleeve,
(Impulse P/No. MCDLS-F)

C-FLUOR Pins
and Wire Color

3

4

2

1

5 6 3

4

2

1

5 3 4

2

1

5

3 3

4 4

2 2

1

5 5

Pigtail Connector

and Wire Color

3

6

4

2 1 5

5

2

4

4

6 1 3

3

6

4

2 1 5

5

2

4

4

6 1 3

3 3

6 6

4 4

2 2 1 5 5

5

2

4

4

6 1 3

Note: A maximum cable length of 30 feet can be used for digital
data transfer from Digital C-FLUOR Probes to computers or data
loggers. For long distance data transfer or communication, the
digital RS232 signal should be converted to RS422.

Digital C-FLUOR Submersible Probe

998-2131 Rev. 1.0 Page 20

Appendix E: Digital C-FLUOR Probe Command List

Digital C-FLUOR Probes can be programmed using commands via serial
communication with terminal emulators such as Hyperterminal, TerraTerm, PuTTY,
Procomm Plus, AlphaCom, etc or third-party communication systems. This is helpful

for integrators as they don’t need software for programming integrated probes. Use

the following settings for serial connection:

Baud Rate: Use the rate that was displayed in the Digital C-FLUOR Software.
Data bits: 8
Parity: None
Stop bits: 1
Flow Control: None

Once connected, you can communicate using the list of commands below to setup,
collect information, and program Digital C-FLUOR Probes:

Command

Name

Note

I!

Sensor Information

For Probe Information

C!

Calibration Information

For Calibration Values

Bxxxx!

Set Baud Rate

“xxxx” refers to baud rate

(9600, 14400, 19200, 38400, 57600)

Sxx!

Streaming Output Rate

Puts probe into streaming mode Use

table below to determine “xx”

P!

Polling Mode

Puts probe into polling mode

R!

Read Command

Only when in Polling mode

M!

Report the Current Mode

Returns S with rate or P

xx

Readings per second

Seconds between

01

Not Available

02

16 03 8

04 4

05 2

06

1

1 (factory default)

07 2

08 5

09 10

10 15

11 30

12 60

13 120

14 300

15 600

16 1800

17 3600