Contents
1. Information ..................................................................................................... 1
2. Before Imaging ................................................................................................ 2
2.1 Check your sample with an ordinary fluorescence microscope ............................. 2
2.2 Clean your slides ................................................................................................. 2
2.3 Check the environment ....................................................................................... 2
2.4 Start the heating earlier if possible ...................................................................... 2
2.5 Cancellation ....................................................................................................... 2
2.6 Unsure? .............................................................................................................. 2
3. Working with immersion objectives ................................................................. 3
4. Starting up and shutting down the instrument ................................................. 4
4.1 Start up .............................................................................................................. 4
4.1.1 Definite focus ........................................................................................................ 4
4.1.2 Main switches ....................................................................................................... 4
4.1.3 Argon laser ............................................................................................................ 5
4.2 Shut down .......................................................................................................... 5
5. Starting the Zen software ................................................................................ 6
5.1 Start the ZEN software ........................................................................................ 6
6. Using the fluorescence microscope .................................................................. 7
6.1 Visualizing the sample in ocular mode ................................................................. 7
6.2 Using the confocal microscope settings ............................................................... 8
6.2.1 Reusing settings from previous experiments ....................................................... 8
6.2.2 Setting up new experiment .................................................................................. 8
6.2.3 Using Smart Setup ................................................................................................ 8
6.2.4 Manually create light paths .................................................................................. 9
6.2.5 Visualise sample on screen ................................................................................... 9
6.2.6 Adjust the Channels ............................................................................................ 10
6.2.7 Acquire images ................................................................................................... 10
6.3 Experiments ..................................................................................................... 11
6.4 Z-stacks ............................................................................................................ 11
7. Live Cell experiments ..................................................................................... 12
7.1 Live imaging equipment set up .......................................................................... 12
7.2 Setting up time-lapse ........................................................................................ 13
8. Troubleshooting ............................................................................................ 14
8.1 Do not see fluorescence in the Locate mode ...................................................... 14
8.2 Weak signal with the Argon laser ...................................................................... 14
8.3 Argon does not start ......................................................................................... 14