THORLABS Cerna Series, SFM2, SFM, SFMGFP, SFMGFP2 User Manual

Cerna® Mini Microscopes
User Guide
Cerna Mini Microscopes
Table of Contents
Chapter 1 Warning Symbol Definitions ............................................................ 1
Chapter 2 Safety ................................................................................................. 2
Chapter 3 Description ........................................................................................ 3
Chapter 4 Getting Started .................................................................................. 7
4.3.1 Microscope .................................................................. 9
4.3.2 Fluorescence Filter Installation .................................. 12
4.3.3 LED Power Source Installation .................................. 13
4.3.4 Camera ..................................................................... 14
Chapter 5 Maintaining the SFM Series ........................................................... 15
Chapter 6 Specifications ................................................................................. 16
6.5 LED Collimation Module Compatibility
(SFM/SFMGFP/SFMGFP2) ................................ ................... 18
Chapter 7 Mechanical Drawings ..................................................................... 27
Cerna Mini Microscopes
Chapter 8 Warranty .......................................................................................... 35
Chapter 9 Regulatory ....................................................................................... 36
Chapter 10 Thorlabs Worldwide Contacts ..................................................... 37
Cerna Mini Microscopes Chapter 1: Warning Symbol Definitions
Symbol
Description
Direct Current
Alternating Current
Both Direct and Alternating Current
Earth Ground Terminal
Protective Conductor Terminal
Frame or Chassis Terminal
Equipotentiality
On (Supply)
Off (Supply)
In Position of a Bi-Stable Push Control
Out Position of a Bi-Stable Push Control
Caution: Risk of Electric Shock
Caution: Hot Surface
Caution: Risk of Danger
Warning: Laser Radiation
Caution: Spinning Blades May Cause Harm
Chapter 1 Warning Symbol Definitions
Below is a list of warning symbols you may encounter in this manual or on your device.
Rev. D, July 9, 2018 Page 1
Cerna Mini Microscopes Chapter 2: Safety
Chapter 2 Safety
All statements regarding safety of operation and technical data in this user guide will only apply when the unit is operated correctly. Refer to the relevant User Guide of the individual product for safety instructions. Please read the following warnings and cautions carefully before operating the unit.
WARNING
DO NOT use the device for anything other than its intended use. If the
device is used in a manner not specified by Thorlabs, the protection
provided by the equipment may be impaired.
CAUTION
DO NOT operate in a wet, damp, or explosive environment.
Page 2 TTN109666-D02
Cerna Mini Microscopes Chapter 3: Description
Chapter 3 Description
The Cerna Mini Microscopes provide simple microscope configurations that are ideal for experiments requiring simple imaging. Thorlabs offers four microscope configurations: SFM2 (Cerna Mini Microscope), SFM (Cerna Mini Microscope with Epi-Illuminator Module), SFMGFP (Cerna Mini Microscope with Epi-Illuminator Module for GFP/Alexa Fluor® 488 with CCD Camera), and SFMGFP2 (Cerna Mini Microscope with Epi-Illuminator Module for GFP/Alexa Fluor® 488 with sCMOS Camera).
The SFM2 consists of a motorized microscope body and camera port. Similarly, SFM, consists of the same base components with the addition of an illumination cube housing module (WFA2002). Both allow you to add your own camera, filter cube, and light source to suit your experimental needs. The motorized objective holder provides 1" of vertical focusing adjustment for the 10X objective. The motorized Microscope Body has ample space beneath the objective for adding sample stages. The C-Mount threaded Camera Port is compatible with most industry-standard cameras.
The SFMGFP and SFMGFP2 microscopes each consist of a motorized microscope body, a camera port, a filter cube, a light source, an LED collimation module, an objective, a scientific camera, and an LED driver.
The epi-illuminator module accepts a variety of Thorlabs’ mounted LEDs. Currently, we offer two types of LED collimation modules (SFM22 and SFM80) to connect the LEDs to the epi-illuminator module. The SFMGFP and SFMGFP2 each ship with an SFM80 module. For two LED collimation modules, replace the SFM Epi adapter at the rear of the WFA2002 module with a DFM1 fluorescence filter cube, a C4W-CC cage cube connector, and an SM1CP2 end cap.
3.1 Features at a Glance
Cerna Mini Features
o Microscope Body Uses 66 mm Optical Rail, Enabling the
Addition of Custom Modules
o 1" of Motorized Microscope Translation in X and Y Axes o Microscope Body with 6" Throat Depth o Coarse Objective Height Adjustment of 6.38"
Additional SFM Microscope Feature
o Epi-illumination filter cube housing (WFA2002)
Additional SFMGFP/SFMGFP2 Microscope Features
o Detection
SFMGFP: 1.4 Megapixel Monochrome Scientific-
Grade CCD Camera
SFMGFP2: 2.1 Megapixel Monochrome sCMOS
Scientific Camera
Connects to 1X Magnification Camera Tube with C-
Mount Threads
2/3" Sensor Format (11 mm Diagonal)
Rev. D, July 9, 2018 Page 3
Cerna Mini Microscopes Chapter 3: Description
3-Axis Controller Box
3-Axis
Knob Box
Filter Cube Housing
Camera Port
Focus
Module
3-Axis Controller Box
o 10X Magnification Objective o Filter Cube with Filter Set for GFP o Epi-Illuminator Module for LED with 80° to 120° Divergence
Angle
o Blue LED for GFP Excitation
470 nm Nominal Wavelength  710 mW Typical Output Power at Max Current  25 nm FWHM (Full Width at Half Max) Bandwidth
3.2 Overview
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Figure 1 SFM Microscope
Cerna Mini Microscopes Chapter 3: Description
3-Axis Controller Box
3-Axis Controller Box
3-Axis
Knob Box
LED
Filter Cube Housing
N10X-PF
T-Cube
Camera Port
SFM80
Monochrome Gig-E Camera
Focus
Module
T-Cube LED Driver
3-Axis
Knob Box
Focus
Module
Camera Port
3-Axis Controller Box
Figure 2 SFM2 Microscope
Rev. D, July 9, 2018 Page 5
Figure 3 SFMGFP Microscope
Cerna Mini Microscopes Chapter 3: Description
3-Axis Controller Box
3-Axis Controller Box
3-Axis
Knob
Box
LED
Filter Cube Housing
N10X-PF
T-Cube
Camera Port
SFM80
Focus
Module
T-Cube LED Driver
sCMOS Camera
Page 6 TTN109666-D02
Figure 4 SFMGFP2 Microscope
Cerna Mini Microscopes Chapter 4: Getting Started
Ordering Codes
Description
SFM
Cerna Mini Microscope with Epi-Illuminator Module
SFM2
Cerna Mini Microscope
SFMGFP
Cerna Mini Microscope with Epi-Illuminator Module for
GFP/Alexa Fluor® 488 with CCD Camera
SFMGFP2
Cerna Mini Microscope with Epi-Illuminator Module for
GFP/Alexa Fluor® 488 with sCMOS Camera
SFM22
22° Collimating Tube with XY Adjustment
SFM80
80° Collimating Tube with XY Adjustment
Chapter 4 Getting Started
This section is provided for those interested in getting the Cerna Mini Microscopes up and running quickly.
4.1 Ordering Codes
4.2 Unpacking and Inspection
Open the package, and carefully remove the Cerna Mini Microscope and its accessories. The table lists the standard accessories shipped with the device.
Rev. D, July 9, 2018 Page 7
Cerna Mini Microscopes Chapter 4: Getting Started
Name
Quantity
SFM
SFM2
SFMGFP
SFMGFP2
Microscope Body with PLS-XY Stage and Motorized Objective Mount
1 1 1
1
3-Axis Controller with Motorized Stage Control Box (Item # MCM3001)
1 1 1
1
Filter Cube Housing (Item # WFA2002)
1 - 1
1
1X Camera Port (Item # WFA4100)
1 - 1
1
MDF-GFP Filter Set
- - 1
1
T-Cube LED Driver (Item # LEDD1B)
- - 1
1
T-Cube Power Supply (Item # KPS101)
- - 1
1
Monochrome Gig-E Camera (Item # 1501M-GE)
- - 1
-
sCMOS Camera (Item # CM2100M­USB)
- - -
1
80° Collimating Tube with XY Adjustment (Item # SFM80)
- - 1
1
Nikon Plan Fluorite Imaging Objective (Item # N10X-PF)
- - 1
1
LED, Blue 470 nm (Item # M470L3)
- - 1
1
OEM Microscopy Filter Cube (Item # MDFM-MF2)
- - 1
1
M6 Socket Head Cap Screw
8 6 8
8
1/4"-20x1/2" Socket Head Cap Screw
8 6 8
8
Name
Quantity
SFM
SFM2
SFMGFP
SFMGFP2
M6 Flat Washer
8 6 8
8
LED Driver Operation Manual
- - 1
1
Thorlabs Digital Camera Quick Start Guide
- - 1
1
ThorCam Camera Software CD
- - 1
1
MCM3000 Series 3-Axis Controller User Guide
1 1 1
1
Inspect the device and its accessories for any missing parts or damage. If there is any problem, please contact our nearest office (see Thorlabs Worldwide Contacts on page 37 for details).
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Cerna Mini Microscopes Chapter 4: Getting Started
Shipping Plates (3X)
18 X 2-56 x 1/4"
Screws
4.3 Cerna Mini Microscope Setup
Note: To set up the SFM and SFM2 microscopes, follow the procedure in Section
4.3.1. For full functionality, the SFM will require the user to purchase an objective, light source, filter cube, fluorescence filter set, collimator and digital camera. The SFM2 will require an objective, light source, and digital camera.
Requirements: The following additional accessories (not included) are required for installation of the microscope:
1.5 mm Hex Key  2 mm Hex Key  2.5 mm Hex Key  5/64" Hex Key
4.3.1 Microscope
1. Use four SHCS (Socket Head Cap Screw) (M6 for metric, 1/4"-20 x 1/2" for imperial) and washers to mount the Microscope Body onto the optical table/breadboard.
2. Use a 5/64" hex key to remove the three shipping plates on the Microscope Body.
3. Skip this step for SFM2. For SFM, use a 2 mm hex key to mount the Filter Cube Housing onto the Dovetail Plate.
Rev. D, July 9, 2018 Page 9
Figure 5 Removing the Shipping Plates
Cerna Mini Microscopes Chapter 4: Getting Started
M4 x 12 mm Screw
M4 x 12 mm Screw
Figure 6 Mounting the Filter Cube Housing
4. For SFM, use the 2 mm hex key to mount the Camera Port onto the Filter Cube Housing. For SFM2, mount the Camera Port directly to the Dovetail Plate.
5. Use two SHCS to mount the 3-Axis Controller Box onto the optical table/breadboard. (Refer to MCM3000 Series 3-Axis Controller User Guide for setup.)
Page 10 TTN109666-D02
Figure 7 Mounting the Camera Port
Cerna Mini Microscopes Chapter 4: Getting Started
6. Connect the adapter cables from the Focus Module and PLS-XY Stage to the 3-Axis Knob Box.
7. Connect the cable from the 3-Axis Knob Box to the 3-Axis Controller Box.
8. Connect the power supply from the 3-Axis Controller Box to a standard power outlet.
9. Mount the objective (included with SFMGFP and SFMGFP2 only) onto the microscope.
Rev. D, July 9, 2018 Page 11
Cerna Mini Microscopes Chapter 4: Getting Started
2X M2 x 5 mm Screws
M3 x 10 mm Screw
4.3.2 Fluorescence Filter Installation
The SFMGFP and SFMGFP2 each ship with an MDF-GFP filter. Note: The SFM module does not come with a filter cube nor a filter set. Use filter
cube item # MDFM-MF2 to install your filter set in the Filter Cube Housing. To install the MDF-GFP filter:
Figure 8 Replacing the Filter Set
1. Use a 1.5 mm hex key to remove the screws (2X) from either side of the Filter Cube Housing cover.
2. Use a 2.5 mm hex key to loosen the screw at the center of the cover to slide out the filter cube.
3. Install your filter set inside the filter cube. See the MDFM-MF2 manual for detailed instructions- https://www.thorlabs.com/manuals.cfm
4. Slide the filter cube on the Filter Cube Housing cover and tighten the screw at the center of the cover.
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Cerna Mini Microscopes Chapter 4: Getting Started
4.3.3 LED Power Source Installation
For easy installation, the Filter Cube Housing included in the SFMGFP and SFMGFP2 configurations comes with a pre-installed LED and SFM80 collimation tube.
1. Use two SHCS to mount the T-Cube LED Driver onto the optical table/breadboard.
2. Connect the LED to the T-Cube LED Driver.
3. Connect the power supply from the T-Cube LED Driver to a standard power outlet.
Rev. D, July 9, 2018 Page 13
Cerna Mini Microscopes Chapter 4: Getting Started
4.3.4 Camera
Mount the camera (Monochrome Gig-E camera for SFMGFP or sCMOS camera for SFMGFP2) on the Camera Port. Refer to the included camera user guide for setup and installation.
Figure 9 Mounting the Camera
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Cerna Mini Microscopes Chapter 5: Maintaining the SFM Series
Chapter 5 Maintaining the SFM Series
There are no serviceable parts in the SFM series modules. If you suspect a problem with your module, please contact our nearest office (see Thorlabs Worldwide Contacts on page 37 for details) for assistance from an applications engineer.
5.1 Cleaning
Refer to the relevant section in the User Guide of individual products for cleaning and maintenance.
WARNING
Disconnect the power supply before cleaning the unit to prevent any
injuries.
CAUTION
DO NOT expose the unit to spray, liquids, or solvents. This may
damage the unit.
Use a damp lint-free cloth to clean the microscope body.
Rev. D, July 9, 2018 Page 15
Cerna Mini Microscopes Chapter 6: Specifications
Specification
Value
Rail Length
174.3 mm (6.86")
Post Length
200 mm (7.87")
Post Manual Height Adjustment
162 mm (6.38”)
Throat Depth
152.4 mm (6")
Fine Motorized Motion
X,Y, and Z Axes
Fine Motorized Total Travel
1″ for Each Axis
Mounting Screw
1/4″-20 or M6
Objective Thread Adapter (Included)
External M32 x 0.75 Threads
Internal M25 x 0.75 Threads
Optional Breadboard (Not Included)
MB1224 or MB3060/M
Specification
Value
Item #
WFA4100
Magnification
1X
Dovetail
Male D1N
Tube Lens Focal Length
200 mm
Camera Threading
C-Mount (1.00″-32)
Specification
Value
Travel Range
25.4 mm (1") for Each Axis
Bidirectional Repeatability
1 µm
Backlash
1 µm
Minimum Achievable Incremental Movement
100 nm
Minimum Achievable Repeatable Movement
200 nm
Velocity (Max)
7 mm/s
Acceleration (Max)
11 mm/s2
Cable Length
6ꞌ (1.8 m)
Chapter 6 Specifications
6.1 Microscope Body Specifications
6.2 Camera Port
6.3 Motorized XYZ Translation
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Cerna Mini Microscopes Chapter 6: Specifications
Stepper Motor Specifications
Thread Screw Pitch
0.3 mm
Step Angle
1.8°
Limit Switches
Hall Effect Sensors
Phase Current
0.49 A
Phase Resistance
5.1 Ω
Phase Inductance
1.5 mH
Insulation Resistance
20 MΩ
Specification
Value
Item #
MCM3001
Motor Output
Motor Drive Voltage
24 V
Motor Drive Current
7.0 A (Peak)
3.0 A (RMS)
Motor Drive Type
12-Bit PWM Control
Control Algorithm
Open-Loop Microstepping
Stepping
64 Microsteps per Full Step
Encoder Resolution
0.212 µm
Total Steps per Revolution
12800
Maximum Stepping Velocity
4577 steps/s
Position Feedback
Quadrature Encode (QEP) Input, 5 V
Encoder Feedback Bandwidth
16 MHz
Position Counter
32-Bit
Operating Modes
Position and Velocity
Velocity Profile
Trapezoid
Motor Drive Connector
Mechanical Specifications
15 Position D-Type, Micro-D Plug, Male Pin
Connector
Motor Drive Outputs
200
Quadrature Encoder (QEP) Input
Single Ended
Limit Switch Inputs
Forward, Reverse, Index
Encode Supply
5 V
Input Power Requirements
Voltage
24 VDC
Current
3.75 A (Peak)
6.4 Motion Controller
Rev. D, July 9, 2018 Page 17
Cerna Mini Microscopes Chapter 6: Specifications
General
Computer Connection
USB 2.0
Housing Dimensions (W x D x H)
97.3 mm x 50.8 mm x 73.6 mm (3.83″ x 2.00″ x 2.90″)
Collimation Module Item #
Compatible LEDs
SFM80 (Only Included with SFMGFP/SFMGFP2)
For Mounted LEDs with 80 - 120° Viewing
Angle, 350 - 700 nm Wavelength
SFM22
For Mounted LEDs with <22° Viewing Angle,
400 - 700 nm Wavelength
Specification
Value
Item #
M470L3
Color
Blue
Nominal Wavelength
470 nm
LED Power Output (Min)
650 mW
LED Power Output (Typical)
710 mW
Maximum Current (CW)
1000 mA
Forward Voltage
3.2 V
Bandwidth (FWHM)
25 nm
Irradiance (Typical)
21.9 µW/mm
2
Electrical Power
3.200 W
Viewing Angle (Full Angle at Half Max)
80°
Emitter Size
1 mm x 1 mm
Typical Lifetime
100000 h
Specification1
Value
Common Data
Item #
LEDD1B
LED Power Supply Item #
KPS101
Output Current Range
0 - 1200 mA
LED Current Limit Set Point Range
200 - 1200 mA
1
6.5 LED Collimation Module Compatibility (SFM/SFMGFP/SFMGFP2)
6.6 LED Specifications (SFMGFP/SFMGFP2)
6.7 T-Cube LED Driver Specifications
All the technical data is valid at 23 ± 5 °C and 45 ± 15% relative humidity.
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Cerna Mini Microscopes Chapter 6: Specifications
LED Forward Voltage
11 V (Min)
12 V (Typical)
Current Ripple
8 mA
Current Ripple Frequency
570 kHz
Modulation Mode2
Frequency Range
0 - 5 kHz, Sine Wave
Modulation Form
Arbitrary
Input Voltage Rate
0 - 5 V
Slew Rate
13.6 mA/µs
Decay Rate
13.1 mA/µs
Trigger Mode2
Frequency Range
0 - 1 kHz
Duty Cycle Range
20 - 80% @ 1 kHz
2 - 98% @ 100 Hz
0.2 - 99.8% @ 10 Hz
Modulation Form
Square Wave / PWM
Logic Input Levels
TTL
Min H-Level: 2 V
Max L-Level: 0.55 V
Slew Rate
18 mA/µs
Rise Time
51 µs
Decay Rate
12 mA/µs
Fall Time
79 µs
General Data
Power Supply
15 VDC
Maximum Power Consumption
15 VA
Operating Temperature
0 to 40 °C
Storage Temperature
-40 to 70 °C
Weight
240 g
Dimensions (W x H x D)
60 mm x 73 mm x 104 mm
2
Specifications for the modulation and trigger modes depend on the forward voltage and capacitance of
the connected LED.
Rev. D, July 9, 2018 Page 19
Cerna Mini Microscopes Chapter 6: Specifications
Specification
Value
Excitation
Filter
Emission
Filter
Dichroic Filter
Item #
MDF-GFP
Size
Ø25 ± 0.1 mm
Ø25 ± 0.1 mm
25.0 mm x 36.0 mm
Clear Aperture
>Ø21 mm
>Ø21 mm
>(22.5 mm x 32.4 mm)
Angle of Incidence
0° ± 5°
0° ± 5°
45° ± 1.5°
Thickness
5.0 ± 0.1 mm
3.5 ± 0.1 mm
1.0 ± 0.1 mm
Surface Quality
60-40 Scratch-Dig
Substrate
Fused Silica
Center Wavelength
469 nm
525 nm
-
FWHM
35 nm
39 nm
-
Reflection Band
-
-
(R
avg
> 90%) 452 - 490
nm
Transmission Band
-
-
(T
abs
> 90%) 505 - 800
nm
AR Coating3
-
-
(R
abs
< 2%) 400 - 800
nm
Specification
Value
Item #
N10X-PF
Magnification
10X
Manufacturer Part #
MRH00101
Numerical Aperture (NA)
0.3
Working Distance (WD)4
16 mm
Parfocal Length4
60 mm
Compatible Tube Lens Focal Length
200 mm
Coverslip Correction
0.17 mm
Diameter4
30.0 mm
Length4
48.7 mm
Threading
M25 x 0.75
3 4
6.8 Fluorescence Filter Specifications (SFMGFP/SFMGFP2)
6.9 Objective Specifications (SFMGFP/SFMGFP2)
Designed for 45° AOI on the back surface. These dimensions are defined in the drawing below.
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Cerna Mini Microscopes Chapter 6: Specifications
Specification
Value
Item #
5
1501M-GE
Sensor Type
Sony ICX285AL Monochrome CCD
(Grade 0)
Effective Number of Pixels (Horizontal x Vertical)
1392 x 1040
Imaging Area (Horizontal x Vertical)
8.98 mm x 6.71 mm
Pixel Size
6.45 µm x 6.45 µm
Optical Format
2/3" Format (11 mm Diagonal)
Peak Quantum Efficiency
Monochrome: 60% at 500 nm
Exposure Time
0 to 1000 seconds in 1 ms Increments
6
CCD Pixel Clock Speed
20 MHz or 40 MHz
Analog-to-Digital Converter Gain
0 to 1023 Steps (0.036 dB/Step)
Optical Black Clamp
0 to 1023 Steps (0.25 ADU/Step)
7
Vertical Hardware Binning
8
Continuous Integer Values from 1 to 24
Horizontal Software Binning
8
Continuous Integer Values from 1 to 24
Region of Interest
1 x 1 Pixel to 1392 x 1040 Pixels,
Rectangular
Read Noise
9
<7 e- at 20 MHz
5
6
7 8 9
Figure 10 Objective Dimensional Drawing
6.10 Monochrome CCD Camera Specifications (SFMGFP)
The specified performance is valid when using a computer with the specifications listed in the
Recommended System Requirements table given below.
Exposure time varies with operating mode; exposure times shorter than 1 ms may be possible when
using an external trigger.
ADU = Analog-to-Digital Unit Camera Frame Rate is impacted by the Vertical Hardware Binning parameter. If your application is read-noise limited, use the lower CCD pixel clock speed of 20 MHz.
Rev. D, July 9, 2018 Page 21
Cerna Mini Microscopes Chapter 6: Specifications
Digital Output
14 Bit
Cooling
None
Host PC Interface
Gigabit Ethernet
Lens Mount
C-Mount (1.000″-32)
Recommended System Requirements
Operating System
Windows 7, 8.1, or 10 (64 bit)
Processor (CPU)
10
3.0 GHz Intel Core (i5 or Higher)
Memory (RAM)
≥8 GB
Hard Drive
500 GB (SATA) Solid State Drive (SSD)
11
Graphics Card
Dedicated
12
Adapter with ≥256 MB RAM
Power Supply
≥600 W
Motherboard
One Unused PCIe x 1 Slot
Connectivity
USB or Internet Connectivity
for Driver Installation
Example Frame Rates at 1 ms Exposure Time
Data Rate13
20 MHz
40 MHz
Full Sensor (1392 x 1040)
12 fps
23 fps
Full Sensor, Bin by 2 (696 x 520)
23 fps
41 fps
Full Sensor, Bin by 10 (139 x 104)
77 fps
112 fps
10 11 12 13
Intel Core i3 processors and mobile versions of Intel processors may not satisfy the requirements. We recommend a solid state drive (SSD) for reliable streaming to disk during image sequence storage. On-board/integrated graphics solutions present on Intel Core i5 and i7 processors are also acceptable. Camera Frame Rate is impacted by the Vertical Hardware Binning parameter.
Page 22 TTN109666-D02
Cerna Mini Microscopes Chapter 6: Specifications
Figure 11 Quantum Efficiency of 1501M-GE Camera
Rev. D, July 9, 2018 Page 23
Cerna Mini Microscopes Chapter 6: Specifications
Figure 12 IR-Blocking Filter Transmission of 1501M-GE Camera
Page 24 TTN109666-D02
Cerna Mini Microscopes Chapter 6: Specifications
Specification
Value
Item #14
CM2100M-USB
Sensor Type
Monochrome sCMOS
Effective Number of Pixels (Horizontal x Vertical)
1920 x 1080
Imaging Area (Horizontal x Vertical)
9.6768 mm x 5.4432 mm
Pixel Size
5.04 µm x 5.04 µm
Optical Format
2/3" (11 mm Diagonal)
Maximum Frame Rate
50 fps (Full Sensor)
Sensor Shutter Type
Rolling
Peak Quantum Efficiency
61% at 600 nm
Removable Window Coating Reflectance
R
avg
<0.5% over 400 - 700 nm (Per
Surface)
Exposure Time
0.029 ms to 7767.2 ms in ~0.03 ms Increments
Pixel Clock Speed
74.25 MHz to 148.5 MHz
ADC15 Resolution
16 Bit
Vertical and Horizontal Hardware Binning
Continuous Integer Values from 1 to
16
Region of Interest (ROI)
8 x 2 Pixels16 to 1920 x 1080 Pixels,
Rectangular
Read Noise
<1 e- Median17
Digital Output
16 Bit
Dynamic Range
Up to 87 dB
Full Well
≥23 ke
-
Lens Mount
C-Mount (1.000"-32)
USB Power Consumption
3.7 W @ 30 fps (Full Sensor ROI)
4.3 W @ 50 fps (Full Sensor ROI)
Recommended System Requirements
Operating System
Windows 7, 8.1, or 10 (64 bit)
Processor (CPU)
18
3.0 GHz Intel Core (i5 or Higher)
Memory (RAM)
≥8 GB
Hard Drive
500 GB (SATA) Solid State Drive (SSD)
19
14
15 16 17 18 19
6.11 sCMOS Camera Specifications (SFMGFP2)
The specified performance is valid when using a computer with the specifications listed in the
Recommended System Requirements table given below.
ADC = Analog to Digital Converter When Binning at 1 x 1 As specified by the sensor manufacturer. The RMS read noise is <1.5 e-. Intel Core i3 processors and mobile versions of Intel processors may not satisfy the requirements. We recommend a solid state drive (SSD) for reliable streaming to disk during image sequence
storage.
Rev. D, July 9, 2018 Page 25
Cerna Mini Microscopes Chapter 6: Specifications
Graphics Card
Dedicated
20
Adapter with ≥256 MB RAM
Power Supply
≥600 W
Motherboard
Integrated Intel USB 3.0 Controller
Connectivity
USB or Internet Connectivity
for Driver Installation
Example Frame Rates at 1 ms Exposure Time
Data Rate21
30 fps
50 fps
Full Sensor (1920 x 1080)
30 fps
50 fps
Half Sensor (960 x 540)
60 fps
100 fps
1/10 Sensor (192 x 108)
300 fps
500 fps
20
21
Figure 13 Quantum Efficiency of CS2100M-USB Camera
On-board/integrated graphics solutions present on Intel Core i5 and i7 processors are also
acceptable.
The data rate can be chosen in ThorCam and is a function of the pixel clock speed.
Page 26 TTN109666-D02
Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Chapter 7 Mechanical Drawings
Figure 14 Mechanical Drawing of SFM Module (Side, Front, and Back
View)
Rev. D, July 9, 2018 Page 27
Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 15 Mechanical Drawing of SFM Module (Top and Bottom
View)
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Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 16 Mechanical Drawing of SFM2 Module (Side, Front, and
Back View)
Rev. D, July 9, 2018 Page 29
Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 17 Mechanical Drawing of SFM2 Module (Top and Bottom
View)
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Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 18 Mechanical Drawing of SFMGFP Module (Side, Front, and
Back View)
Rev. D, July 9, 2018 Page 31
Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 19 Mechanical Drawing of SFMGFP Module (Top and Bottom
View)
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Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 20 Mechanical Drawing of SFMGFP2 Module (Side, Front, and
Back View)
Rev. D, July 9, 2018 Page 33
Cerna Mini Microscopes Chapter 7: Mechanical Drawings
Figure 21 Mechanical Drawing of SFMGFP2 Module (Top and Bottom
View)
Page 34 TTN109666-D02
Cerna Mini Microscopes Chapter 8: Warranty
Chapter 8 Warranty
Thorlabs warrants that all products sold by Thorlabs will conform to the published specifications and shall be free from defects in material and workmanship under normal use, handling, and service.
Thorlabs provides warranty for a period of one year.
Rev. D, July 9, 2018 Page 35
Cerna Mini Microscopes Chapter 9: Regulatory
Wheelie Bin Logo
Chapter 9 Regulatory
As required by the WEEE (Waste Electrical and Electronic Equipment Directive) of the European Community and the corresponding national laws, Thorlabs offers
all end users in the EC the possibility to return “end of life” units without incurring
disposal charges.
This offer is valid for Thorlabs electrical and electronic equipment:  Sold after August 13, 2005  Marked correspondingly with the crossed out
“wheelie bin” logo (see right)
Sold to a company or institute within the EC  Currently owned by a company or institute
within the EC
Still complete, not disassembled and not
contaminated
As the WEEE directive applies to self-contained operational electrical and electronic products, this end of life take back service does not refer to other Thorlabs products, such as:
Pure OEM products, that means assemblies to be built into a unit by the
user (e.g. OEM laser driver cards)
Components  Mechanics and optics  Left over parts of units disassembled by the user (PCB’s, housings etc.).
If you wish to return a Thorlabs unit for waste recovery, please contact Thorlabs or your nearest dealer for further information.
Waste Treatment is Your Own Responsibility
If you do not return an “end of life” unit to Thorlabs, you must hand it to a company
specialized in waste recovery. Do not dispose of the unit in a litter bin or at a public waste disposal site.
Ecological Background
It is well known that WEEE pollutes the environment by releasing toxic products during decomposition. The aim of the European RoHS directive is to reduce the content of toxic substances in electronic products in the future.
The intent of the WEEE directive is to enforce the recycling of WEEE. A controlled recycling of end of life products will thereby avoid negative impacts on the environment.
Page 36 TTN109666-D02
Cerna Mini Microscopes Chapter 10: Thorlabs Worldwide Contacts
USA, Canada, and South America
Thorlabs, Inc. 56 Sparta Avenue Newton, NJ 07860 USA Tel: 973-300-3000 Fax: 973-300-3600 www.thorlabs.com www.thorlabs.us (West Coast)
Email: sales@thorlabs.com Support: techsupport@thorlabs.com
UK and Ireland
Thorlabs Ltd. 1 Saint Thomas Place, Ely Cambridgeshire CB7 4EX Great Britain Tel: +44 (0)1353-654440 Fax: +44 (0)1353-654444 www.thorlabs.com
Email: sales.uk@thorlabs.com Support: techsupport.uk@thorlabs.com
Europe
Thorlabs GmbH Hans-Böckler-Str. 6 85221 Dachau Germany Tel: +49-(0)8131-5956-0 Fax: +49-(0)8131-5956-99 www.thorlabs.de
Email: europe@thorlabs.com
Scandinavia
Thorlabs Sweden AB Bergfotsgatan 7 431 35 Mölndal Sweden Tel: +46-31-733-30-00 Fax: +46-31-703-40-45 www.thorlabs.com
Email: scandinavia@thorlabs.com
France
Thorlabs SAS 109, rue des Côtes 78600 Maisons-Laffitte France Tel: +33 (0) 970 444 844 Fax: +33 (0) 825 744 800 www.thorlabs.com
Email: sales.fr@thorlabs.com
Brazil Thorlabs Vendas de Fotônicos Ltda. Rua Riachuelo, 171 São Carlos, SP 13560-110 Brazil Tel: +55-16-3413 7062 Fax: +55-16-3413 7064 www.thorlabs.com
Email: brasil@thorlabs.com
Japan
Thorlabs Japan, Inc. 3-6-3, Kitamachi, Nerima-ku, Tokyo 179-0081 Japan Tel: +81-3-6915-7701 Fax: +81-3-6915-7716 www.thorlabs.co.jp Email: sales@thorlabs.jp
China Thorlabs China Room A101, No. 100 Lane 2891, South Qilianshan Road Putuo District Shanghai China Tel: +86 (0) 21-60561122 Fax: +86 (0)21-32513480 www.thorlabschina.cn
Email: chinasales@thorlabs.com
Chapter 10 Thorlabs Worldwide Contacts
Rev. D, July 9, 2018 Page 37
www.thorlabs.com
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