Thermo Scientific Wellwash Versa User Manual
Thermo Scientific
Wellwash® Versa
User Manual
Rev. 1.0
Thermo Scientific
Wellwash
®
Versa
User Manual
Rev. 1.0, Cat. no. N11166
Copyright
Copyright © 2010 Thermo Fisher Scientific Corporation. All rights reserved. Printed in Finland.
Reproduction of the accompanying user documentation in whole or in part is prohibited.
Trademarks
“Nunc” and “Wellwash” are registered trademarks of Thermo Fisher Scientific Inc. and its subsidiaries.
All other trademarks and registered trademarks are the property of their respective holders.
Disclaimer
Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate
technological developments. This manual is subject to change without prior notice as part of a continuous
product development. Although this manual has been prepared with every precaution to ensure accuracy,
Thermo Fisher Scientific assumes no liability for any errors or omissions, nor for any damages resulting from
the application or use of this information. This manual supersedes all previous editions.
Remarks on screenshots
The version number displayed in screenshots may not always be the one of the currently released version.
Screenshots are only replaced if the content related to the application has changed.
No liability for consequential damages
Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to
use this product.
Power failure
The system requires uninterrupted power supply in order to operate correctly. Thermo Fisher Scientific has no
responsibility whatsoever for system malfunctions arising from power failures.
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 3
About This User Manual
The Wellwash Versa microplate washer can be used as standalone in
research and routine-test laboratories by professional personnel.
This user manual is for the following instruments, Wellwash Versa:
Cat. no. 5165010 and 5165030. It has been designed to give you the
information you need for:
● Reviewing safety precautions
● Installing the instrument
● Navigating in the user interface
● Operating the instrument
● Programming of wash protocols
● Defining of wash parameters
● Performing cleaning and maintenance procedures
● Troubleshooting the instrument performance
This user manual also describes all the features and specifications of the
Wellwash Versa instrument as well as ordering information.
Read the manual in its entirety before operating the instrument.
Keep the user manual for future reference. The user manual is an
important part of the instrument and should be readily available during
use of the instrument. Keep the user manual together with the
instrument in case you distribute it onwards.
For the latest information on products and services, visit our websites
at:
http://www.thermoscientific.com
http://www.thermoscientific.com/mpi
http://www.thermoscientific.com/readingroom
http://www.thermoscientific.com/wellwash
In our efforts to provide useful and appropriate documentation, we
appreciate your comments on this user manual to your local Thermo
Fisher Scientific representative.
Intended users
How to use this
user manual
For more
information
About This User Manual
Safety symbols and markings
4 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
These symbols are intended to draw your attention to particularly
important information and alert you to the presence of hazards as
indicated.
The following symbols and markings appear on the type label and the
instrument itself.
Power ON
▲
Power OFF
▲
Serial number
▲
Catalog number
▲
Date of manufacture ▲
Consult instructions for use
▲
WEEE symbol This product is required to comply with the European
Union’s Waste Electrical & Electronic Equipment (WEEE) Directive
2002/96/EC.
▲
A black label with the following text (Figure 2–3 on page 16):
CAUTION: WARNING: DISCONNECT SUPPLY BEFORE
SERVICING and AVERTISSEMENT: COUPER
L'ALIMENTATION AVANT L'ENTRETIEN ET LE
DEPANNAGE.
▲
Safety symbols
and markings
Safety symbols and
markings used on
the Wellwash Versa
About This User Manual
Safety symbols and markings
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 5
The following symbols and markings appear in this user manual.
Warning Risk of electric shock.
▲
Warning Biohazard risk.
▲
Warning Risk of injury to the user(s).
▲
Caution Risk of damage to the instrument, other equipment or loss of
performance or function in a specific application.
▲
Note Marks a hint, important information that is useful in the
optimum operation of the system, or an item of interest.
▲
W
arning and other
markings used in
the documentation
About This User Manual
Safety symbols and markings
6 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 7
Contents
Intended users................................................................................... 3
How to use this user manual ............................................................. 3
For more information .......................................................................3
Safety symbols and markings............................................................. 4
Safety symbols and markings used on the Wellwash Versa .............4
Warning and other markings used in the documentation ...............5
Introduction to the Wellwash
®
Versa Microplate Washer................. 13
Intended use.................................................................................... 13
Wellwash Versa Main Parts...................................................................... 15
Instrument views............................................................................. 15
Liquid system diagram ....................................................................17
USB memory stick port................................................................... 17
USB PC port................................................................................... 17
Plate carrier .....................................................................................17
Wash heads .....................................................................................17
Shaker .............................................................................................19
Installation .................................................................................................... 21
What to do upon delivery ...............................................................21
Unpacking the instrument ...........................................................21
Checking delivery for completeness or damage............................. 21
Environmental requirements ........................................................22
Installation setups............................................................................ 22
Releasing the transport lock.......................................................... 22
Removing the priming vessel........................................................ 24
Installing the wash head(s) ...........................................................24
Liquid containers and channels ....................................................27
Connection diagram.................................................................. 28
Installing the liquid containers ..................................................28
Foaming.................................................................................... 30
Installing the aerosol cover ...........................................................31
Connecting the power supply cable.............................................. 31
Connecting to a computer............................................................... 32
Warnings and cautions.................................................................... 32
Electrical ...................................................................................... 32
Defects and abnormal stresses....................................................... 33
Operating precautions and limitations before operation ..................33
Switching on ...................................................................................34
Performing the operational check.................................................... 35
Settings after installation ................................................................. 35
Chapter 1
Chapter 2
Chapter 3
Contents
8 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Running Wash Protocols ............................................................................37
Filling and emptying liquid containers ............................................38
Selecting a protocol .........................................................................38
Selecting a ready-made protocol ...................................................38
Loading the plate ............................................................................39
Priming the system.......................................................................... 40
Strip selection.................................................................................. 41
Strip selection with the number keys ............................................41
Strip selection with the Layout row ..............................................42
Canceling a run............................................................................... 42
Rinsing the liquid system ................................................................43
Operating the Instrument............................................................................ 45
Display and keys for navigating and editing ....................................45
Menus............................................................................................. 47
Main menu ..................................................................................... 47
Liquid level detection................................................................... 48
Protocol .......................................................................................48
Creating a protocol ...................................................................49
Creating a protocol in an optional way...................................... 53
Adding new steps to protocols................................................... 54
Deleting steps from protocols.................................................... 56
Head / Plate .................................................................................57
Well .............................................................................................58
Microplate requirements ...........................................................59
Steps ............................................................................................60
Wash......................................................................................... 60
Aspirate..................................................................................... 63
Dispense ...................................................................................64
Soak ..........................................................................................65
Prime ........................................................................................ 65
Pause......................................................................................... 66
Layout....................................................................................... 66
Layout row................................................................................... 68
Settings menu .................................................................................68
Prime & rinse parameters............................................................. 68
Sensors .........................................................................................70
Wash head configuration..............................................................70
System.......................................................................................... 71
Reports......................................................................................... 72
Maintenance menu .........................................................................73
Clean ...........................................................................................73
Cleaning/Disinfection procedure............................................... 74
Clean wash head........................................................................ 76
Soak wash head .........................................................................78
Move wash head........................................................................ 80
Empty priming vessel ................................................................81
Calibration................................................................................... 82
Active calibration ......................................................................82
Chapter 4
Chapter 5
Contents
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 9
Calibrate current wash head ...................................................... 83
Service.......................................................................................... 87
Calibrate dispensing pump speed ..............................................87
Exporting and Importing............................................................................. 89
Exporting a protocol .......................................................................89
Importing a protocol....................................................................... 90
Exporting a protocol as text file....................................................... 91
Shutdown....................................................................................................... 93
Shutting down ................................................................................93
Emergency Situations ................................................................................. 95
Handling emergency situations .......................................................95
Maintenance................................................................................................. 97
Regular and preventative maintenance ............................................97
Maintenance checklist ..................................................................97
Instrument care ............................................................................98
Cleaning the instrument.................................................................. 98
Salt deposit................................................................................... 99
Cleaning the plate carrier ................................................................99
Cleaning liquid containers............................................................. 100
Replacing the wash head(s)............................................................ 101
Disposal of materials ..................................................................... 101
Decontamination procedure.......................................................... 102
Refitting the transport lock ...........................................................103
Maintaining a system log............................................................... 105
Packing for service......................................................................... 105
Disposal of the instrument ............................................................105
Technical Specifications ......................................................................... 107
General specifications.................................................................... 107
Performance specifications ............................................................108
Safety specifications....................................................................... 109
In conformity with the requirements..........................................109
Troubleshooting Guide ............................................................................. 111
Error and warning codes ...............................................................111
Troubleshooting guide ..................................................................113
Ordering Information................................................................................. 115
Wellwash Versa............................................................................. 115
List of spare parts and accessories ..................................................115
List of Thermo Scientific plates..................................................... 116
System Log .................................................................................................. 117
Chapter 6
Chapter 7
Chapter 8
Chapter 9
Chapter 10
Chapter 11
Chapter 12
Appendix A
Contents
10 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Certificate of Decontamination ...............................................................119
Appendix B
Contents
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 11
Contents
12 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 13
Chapter 1
Introduction to the Wellwash®
Versa Microplate Washer
The Wellwash Versa (Figure 1-1) is a microplate washer. It is designed
to use both 96- and 384-well plate formats: the 96-well plate in both
landscape and portrait orientation, and the 384-well plate in landscape
orientation. The 96-well plates and strips are designed to be washed in
1 x 8, 2 x 8, 1 x 12, or 2 x 12 format and the 384-well plates in 1 x 16
format. The instrument also allows shaking during soaking. The
instrument has an intuitive graphical user interface. In addition, the
Wellwash Versa can be connected to plate handling devices.
The Wellwash Versa can be used to wash and prepare plates for a
variety of test routines, mainly in enzyme-linked immunosorbent assay
(ELISA) tests.
The Wellwash Versa is available in the following configurations:
● Wellwash Versa 100-240V (Cat. no. 5165010)
● 96- and 384-well plate washing
● Wellwash Versa 100-240V (Fisher Scientific, Cat. no. 5165030)
● 96- and 384-well plate washing
Figure 1-1. Wellwash Versa microplate washer
The Wellwash Versa is a microplate washer intended for automated
washing, aspiration, dispensing and shaking of 96- and 384-well plates
and strips that meet ANSI/SBS standards. The instrument can be
Intended use
Introduction to the Wellwash® Versa Microplate Washer
Intended use
14 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
equipped with up to four liquid containers. It can be used in research or
routine-test laboratories by professional personnel.
The Wellwash Versa is part of an analyzing system for the end user,
who is thus responsible for validation of the whole system in order to
produce reliable and safe results. If the analyzing performance is critical
for medical diagnosis, the diagnostic test result has to be checked using
internal quality controls or with a corresponding test. We recommend
using Good Laboratory Practices (GLP) during the analyzing process.
Use for self-testing is excluded.
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 15
Chapter 2
Wellwash Versa Main Parts
This chapter describes the main parts of the Wellwash Versa
instrument.
The front view of the Wellwash Versa instrument is shown in
Figure 2–2.
Figure 2–2. Wellwash Versa front view with accessories
The back view of the Wellwash Versa instrument is shown in
Figure 2–3.
Instrument
views
Wash heads on wash head arm
Priming vessel
Keypad
Display
Plate carrier
See-through aerosol cover
Liquid containers
Plate clamp
Bottle stand (option)
Wellwash Versa Main Parts
Instrument views
16 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Figure 2–3. Wellwash Versa back view
The side views of the Wellwash Versa instrument are shown in
Figure 2–4.
Figure 2–4. Wellwash Versa side views
Connectors for liquid container tubes,
from left to right:
W (Waste), Rinse, B (Buffer B), and A (Buffer A)
ON/OFF switch
Power supply connector
USB port for PC
USB port for memory stick
ON/OFF switch
USB port for memory stick
Connectors for liquid
sensor cables,
from top to bottom:
W (Waste),
Rinse,
B (Buffer B), and
A (Buffer A)
Left
Right
Serial RS-232 C port
Wellwash Versa Main Parts
Liquid system diagram
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 17
Dispense valves
Dispense pump
Liquid level sensors
Liquid containers
Waste container
Wash head manifolds
Liquid valve manifold
Aspirate pump
Liquid level sensor
RBA
The instrument is equipped with a USB port (host) for an external
memory stick (Figure 2–4). You can transfer wash protocols from one
instrument to another of the same model with the USB memory stick.
The instrument is equipped with a USB port (client) for an external PC
connection. The connection is used for remote control and service of
the instrument. A separate manual, Wellwash Versa Remote Control
Command Sets (Cat. no. D08894), is available upon request.
The plate carrier of the Wellwash Versa instrument supports both
portrait and landscape orientation of the plate (Figure 2–2). A plate
clamp is incorporated into the plate carrier to keep the plate firmly
positioned during processing. A sensor in the plate clamp senses the
presence of a microplate.
The wash head alternatives (Figure 2–5) and their configurations
(Figure 2–6) are shown below.
Liquid system
diagram
USB memory
stick port
USB PC port
Plate carrier
W
ash heads
Wellwash Versa Main Parts
Wash heads
18 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Figure 2–5. Wellwash Versa wash head models
Figure 2–6. Configurations of Wellwash Versa wash heads
Wellwash Versa Main Parts
Shaker
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 19
The linear shaker operates at three different speeds (Table 2–1):
Table 2–1. Shaking speeds
Speed designation Speed
Low 5 Hz, amplitude 2.5 mm
Medium 10 Hz, amplitude 1.5 mm
High 15 Hz, amplitude 1 mm
Shaker
Wellwash Versa Main Parts
Shaker
20 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 21
Chapter 3
Installation
This chapter describes the installation procedures for the Wellwash
Versa instrument.
Warning The Wellwash Versa weighs 9 kg [19.8 lbs.] and care must be
taken when lifting the instrument.
▲
This section covers the relevant procedures to be carried out on receipt
of the instrument.
Move the packed instrument to its site of operation. To prevent
condensation, the instrument should be left in its protective plastic
wrapping until the ambient temperature has been reached. Unpack the
Wellwash Versa instrument and accessories carefully with the arrows on
the transport package pointing upwards. Place the instrument onto a
laboratory bench.
Caution Do not touch or loosen any screws or parts other than those
specifically designated in the instructions. Doing so may cause
misalignment and will invalidate the instrument warranty.
▲
Retain the original packaging for future transportation. The packaging
is designed to assure safe transport and minimize transit damage. Using
other packaging materials may invalidate the warranty. Also retain all
instrument-related documentation provided by the manufacturer for
future use.
If you relocate your instrument or ship it for service, refer to “Packing
for service” on page 105.
Check the enclosed packing list against the order. Visually inspect the
transport package, the instrument and the accessories for any possible
transport damage. If any parts are missing or damaged, contact your
local Thermo Fisher Scientific representative or Thermo Fisher
Scientific Oy.
W
hat to do upon
delivery
Unpacking the
instrument
Checking delivery
for completeness or
damage
Installation
Installation setups
22 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
When you set up your Wellwash Versa, avoid sites with excess dust,
vibrations, strong magnetic fields, direct sunlight, draft, excessive
moisture or large temperature fluctuations. Make sure that:
● The working area is flat, dry, clean and vibration-proof, and leave
additional room for cables, covers, and so on
● The ambient air is clean and free of corrosive vapors, smoke and
dust
● The ambient temperature range is between +10°C (50°F) and +40°C
(104°F)
● The humidity is low so that condensation does not occur (relative
humidity is between 10% and 80%, non-condensing).
Caution Do not operate the instrument in an environment where
potentially damaging liquids or gases are present.
▲
This section describes the procedures that must be carried out before
instrument operation.
Caution Leave the instrument to stand for at least three hours before
installing and switching it on, so there is no possibility of condensation
causing a short circuit.
▲
Warning All parts of the instrument that come into contact with
potentially infectious materials must be treated as potentially infectious
areas.
It is advisable to adhere to applicable safety precautions, including the
wearing of powder-free gloves, safety glasses and protective clothing, to
avoid potential infectious disease contamination when performing
cleaning procedures and also when making adjustments to the
instrument.
▲
The instrument comes with one transport lock.
Caution Make sure that the transport lock has been released before you
put the instrument into operation.
▲
To release the transport lock:
1. Remove the padded packing material protecting the wash head arm
and plate carrier to expose the transport lock and transport lock tag
(Figure 3–7).
Environmental
requirements
Installation setups
Releasing the
transport lock
Installation
Installation setups
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 23
Figure 3–7. Transport lock and transport lock tag
2. Unscrew the transport lock screw with the supplied Allen key
(Figure 3–8).
Figure 3–8. Unfastening the transport lock
3. Keep the transport lock and tag (Figure 3–9) for future relocation or
transportation of the instrument.
Figure 3–9. Transport lock and tag removed
Installation
Installation setups
24 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
The instrument comes with the priming vessel installed. The priming
vessel is correctly installed if it stays in place and does not move up.
To remove the priming vessel:
1. Push the priming vessel away from yourself towards the instrument
until you hear a snap.
2. Lift the priming vessel up and remove it (Figure 3–10).
Figure 3–10. Removing the priming vessel
Warning The priming vessel may be infectious after the instrument has
been used. ▲
The wash heads are packed separately and must be installed before use.
Warning The wash heads may be infectious after the instrument has
been used.
▲
Note Always wear disposable powder-free gloves when handling the
wash heads.
▲
To install the wash head(s):
1. Fit the wash heads to the tubing according to Figure 3–11 and
Figure 3–12. Note the difference in sizes.
● The larger thicker tube is the aspiration (waste) tube and is
connected to the bottom hole.
● The smaller tube is the dispensing tube and is connected to
the top hole.
Removing the
priming vessel
Installing the wash
head(s)
Installation
Installation setups
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 25
● Apply silicone grease to the O rings of the tubes if necessary.
● The small and large tube on the left side are blue for easier
recognition.
Figure 3–11. Wash heads (A) and tubes (B)
2. Fit the wash heads next to each other onto the wash arm
(Figure 3–12).
Figure 3–12. Fitting the 2 x 8 wash heads next to each other onto the wash
arm
3. Check that the wash heads are evenly inserted (Figure 3–13).
A
B
Installation
Installation setups
26 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Figure 3–13. 2 x 8 wash heads evenly inserted
4. Ensure that the wash heads are in the correct order (Figure 3–14).
5. If only one wash head (1 x 8 or 1 x 12) is used, place the blank wash
head onto the left of the wash arm.
Figure 3–14. Fitting the blank wash head onto the left of the wash arm
6. Enter the new wash head configuration in the Settings menu to
correspond to the installed wash head.
The information is used to check for protocol wash head
mismatches. Refer to “Wash head configuration” on page 70.
Installation
Installation setups
Thermo Fisher Scientific Thermo Scientific Wellwash Versa User Manual 27
The Wellwash Versa includes three standard buffer containers (2 liters)
and a waste container (4 liters). Containers for greater volumes are
available as an option (4 l for buffers and 10 l for waste).
The liquid channels are: A (for Buffer A), B (for Buffer B), R (for
Rinse), and W (for Waste). Channel R is primarily intended for rinsing
and is connected to the
RINSE key as the only channel. The Rinse
channel can also be used in wash protocols as the buffer source.
The liquid containers are named and the tubing is color coded to
correspond to the correct buffer source (A = blue, B = yellow, R =
green, and W = colorless) (Figure 3–15). Refer to ”Connection
diagram” on page 28.
Figure 3–15. Waste and liquid containers with liquid level detection sensors
Caution Do not cover the venting holes.
▲
Liquid containers
and channels
Venting holes
Installation
Installation setups
28 Thermo Scientific Wellwash Versa User Manual Thermo Fisher Scientific
Figure 3–16 shows the connections of the liquid level sensor cabling and
liquid container tubing.
Figure 3–16. Wellwash Versa connection diagram
To install the liquid containers:
1. Fit the liquid container tubing to the corresponding color-coded
tube connectors on the left side panel of the instrument
(Figure 3–17). The connectors are from left to right: W (Waste), R
(Rinse), B (Buffer B), and A (Buffer A).
Connection diagram
Installing the liquid
containers
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