Thermo Scientific Vanquish VH-P10-A-02 Operating Manual

thermoscientific

Vanquish

Pumps

VH-P10-A-02

Operating Manual

4820.4411-EN Revision 1.0 • October 2017

Copyright © 2017 Thermo Fisher Scientific Inc. All rights reserved.

Original Operating Manual

The hardware descriptions in this manual revision refer to pumps VH-P10-A-02. For the manual
release history, see page 229.

Trademarks

MP35N is a trademark of SPS Technologies. PharMed is a trademark of Saint-Gobain
Performance Plastics. Torx is a trademark of TEXTRON INDUSTRIES, Incorporated. Acrobat,
Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated. Microsoft, Windows,
and Windows Vista are trademarks of Microsoft Corporation.

All other trademarks are property of Thermo Fisher Scientific and its subsidiaries.

Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. The document is copyright protected; any reproduction of the
whole or any part of this document is strictly prohibited, except with the written authorization
of Thermo Fisher Scientific Inc.

This manual is provided "as is." The contents of this manual are subject to being changed,
without notice, in future revisions.

Thermo Fisher Scientific Inc. makes no representations that this document is complete,
accurate, or error-free. Thermo Fisher Scientific Inc. assumes no responsibility and will not be
liable for any errors, omissions, damage, or loss that might result from any use of this document,
even if the information in the document is followed properly.

This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale.
The Terms and Conditions of Sale shall govern all conflicting information between the two
documents.

Printed manual version only

Printed in Germany on 100% chlorine-free bleached, high-white paper that is produced in an
environmentally friendly process, leading to a paper profile of zero CO

emissions.

2

Manufacturer's address

Dionex Softron GmbH, Part of Thermo Fisher Scientific
Dornierstrasse 4, D-82110 Germering

Contacting Us

Ordering Information

Technical Assistance

Contacting Us

There are several ways to contact us:

For ordering information or sales support for HPLC products, contact
your local Thermo Fisher Scientific sales organization. For contact
information, go to Contact Us on http://www.thermofisher.com.

For technical support for HPLC products, contact your local Thermo
Fisher Scientific support organization. For contact information, go to
Contact Us on http://www.thermofisher.com.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 3

Contacting Us

Page 4 Vanquish Pumps (VH-P10-A-02) Operating Manual

Contents

1 Using this Manual ...........................................................................11

2 Safety ..............................................................................................17

Contents

1.1 About this Manual ................................................................................................ 12

1.2 Conventions .......................................................................................................... 13

1.2.1 Safety Messages ....................................................................................... 13

1.2.2 Special Notices and Informational Notes ................................................. 13

1.2.3 Typographical Conventions ...................................................................... 14

1.3 Reference Documentation .................................................................................... 15

2.1 Safety Symbols and Signal Words ......................................................................... 18

2.1.1 Safety Symbols and Signal Words in This Manual ................................... 18

2.1.2 Observing this Manual ............................................................................. 19

2.1.3 Safety Symbols on the Pump ................................................................... 19

2.1.4 Rating Plate .............................................................................................. 20

2.2 Intended Use ......................................................................................................... 21

2.3 Safety Precautions ................................................................................................ 22

2.3.1 General Safety Information ..................................................................... 22

2.3.2 Qualification of the Personnel ................................................................. 23

2.3.3 Personal Protective Equipment ............................................................... 23

2.3.4 Electrical Safety Precautions .................................................................... 24

2.3.5 General Residual Hazards ........................................................................ 25

2.3.6 In Case of Emergency ............................................................................... 26

2.4 Solvent and Additive Information ......................................................................... 27

2.5 Compliance Information ....................................................................................... 29

3 Pump Overview ...............................................................................31

3.1 Pump Features ...................................................................................................... 32

3.2 Operating Principle ............................................................................................... 33

3.3 Interior Components............................................................................................. 35

3.4 Piston Seal Wash System ...................................................................................... 37

3.5 Purge Unit ............................................................................................................. 38

3.6 Leak Detection ...................................................................................................... 39

3.7 Operation .............................................................................................................. 40

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 5

Contents

4 Unpacking ....................................................................................... 41

4.1 Unpacking ............................................................................................................ 42

4.2 Scope of Delivery ................................................................................................. 45

5 Installation ...................................................................................... 47

5.1 Safety Guidelines for Installation ......................................................................... 48

5.2 Installing the Pump .............................................................................................. 49

5.3 Site Requirements ................................................................................................ 51

5.4 Accessing the Interior Components ..................................................................... 53

5.5 Setting Up the Hardware ..................................................................................... 54

5.5.1 System Arrangement............................................................................... 54

5.5.2 Connecting the Pump .............................................................................. 56

5.6 Setting Up the Flow Connections ......................................................................... 59

5.6.1 General Information and Guidelines ....................................................... 59

5.6.2 Guiding Capillaries and Tubing Through the System .............................. 60

5.6.3 Connecting Fittings, Capillaries, and Tubing ........................................... 62

5.6.4 Guiding Liquids to Waste ........................................................................ 64

5.6.5 Connecting the Solvent Lines .................................................................. 65

5.6.6 Setting Up the Seal Wash System ........................................................... 69

5.6.7 Connecting the Pump and Autosampler ................................................. 77

5.7 Turning On the Pump ........................................................................................... 78

5.8 Setting Up the Pump in the Software .................................................................. 79

6 Operation ....................................................................................... 81

6.1 Introduction to this Chapter ................................................................................ 82

6.2 Safety Guidelines for Operation .......................................................................... 83

6.3 Control Elements.................................................................................................. 84

6.3.1 Keypad ..................................................................................................... 84

6.3.2 Status Indicators ...................................................................................... 86

6.4 Power On/Off Control .......................................................................................... 88

6.5 Use of Solvents and Additives .............................................................................. 89

6.6 Preparing the Pump for Operation ...................................................................... 91

6.7 Important Operating Parameters ........................................................................ 93

6.8 Purging the Pump................................................................................................. 98

Page 6 Vanquish Pumps (VH-P10-A-02) Operating Manual

Contents

6.9 Optimizing the Performance of the Pump .......................................................... 101

6.9.1 General Guidelines ................................................................................. 101

6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple .............................. 102

6.9.3 Installing Solvent Shut Off Valves .......................................................... 109

6.10 Shutting Down the Pump .................................................................................... 111

6.10.1 Short-Term Shutdown (Interruption of Operation) ............................... 111

6.10.2 Long-Term Shutdown ............................................................................. 112

7 Maintenance and Service .............................................................. 115

7.1 Introduction to Maintenance and Service .......................................................... 116

7.2 Safety Guidelines for Maintenance and Service ................................................. 117

7.3 General Rules for Maintenance and Service ...................................................... 119

7.4 Routine and Preventive Maintenance ................................................................ 120

7.4.1 Maintenance Plan .................................................................................. 120

7.4.2 Flushing the Pump ................................................................................. 122

7.4.3 Cleaning or Decontaminating the Pump ................................................ 124

7.4.4 Predictive Performance ......................................................................... 126

7.5 Seal Wash System ............................................................................................... 128

7.5.1 Testing the Seal Wash System for Leakage ............................................ 128

7.5.2 Replacing the Seal Wash Lines ............................................................... 129

7.5.3 Replacing the Seal Wash Detector ......................................................... 132

7.6 Y-Connector ........................................................................................................ 134

7.7 Pump Head.......................................................................................................... 136

7.7.1 Overview of Pump Head Parts ............................................................... 137

7.7.2 Replacing the Pump Head ...................................................................... 138

7.7.3 Replacing the Pistons ............................................................................. 142

7.7.4 Cleaning the Piston ................................................................................ 145

7.7.5 Replacing the Piston Seals or Support Ring ........................................... 146

7.7.6 Recommendations for New Piston Seals ............................................... 151

7.7.7 Replacing the Seal Wash Seals ............................................................... 153

7.7.8 Replacing the Pump Head Seals ............................................................. 158

7.7.9 Testing the Piston Seals for Leakage ...................................................... 161

7.8 Check Valves ....................................................................................................... 163

7.8.1 Replacing the Inlet Check Valves ........................................................... 163

7.8.2 Replacing the Outlet Check Valves ........................................................ 165

7.8.3 Cleaning the Check Valves ..................................................................... 166

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 7

Contents

7.9 Inline Filter ......................................................................................................... 167

7.9.1 Testing the Inline Filter for Permeability .............................................. 167

7.9.2 Replacing the Inline Filter or Capillary Mixer ........................................ 168

7.10 Mixing System .................................................................................................... 170

7.10.1 Testing the Static Mixer for Permeability ............................................. 170

7.10.2 Replacing the Static Mixer or Capillary Mixer ....................................... 171

7.11 Solvent Lines and Solvent Line Filters ................................................................ 173

7.11.1 Emptying the Solvent Lines ................................................................... 173

7.11.2 Replacing Solvent Lines ......................................................................... 173

7.11.3 Replacing the Solvent Line Filter ........................................................... 176

7.12 Testing the Pump for Leakage ........................................................................... 178

7.13 Replacing the Main Power Fuses ....................................................................... 180

7.14 Updating the Pump Firmware ............................................................................ 182

7.15 Replacing the Doors ........................................................................................... 184

7.16 Transporting or Shipping the Pump ................................................................... 186

7.16.1 Preparing the Pump for Transport ........................................................ 187

7.16.2 Transporting the Pump to a New Location ........................................... 188

7.16.3 Shipping the Pump ................................................................................ 188

7.17 Replacing the Slide-In Module ........................................................................... 190

7.17.1 Removing the Slide-In Module .............................................................. 190

7.17.2 Installing the Slide-In Module ............................................................... 192

8 Troubleshooting ............................................................................ 195

8.1 General Information about Troubleshooting .................................................... 196

8.2 Messages ............................................................................................................ 199

8.3 Testing From Chromeleon ................................................................................. 207

8.4 Resolving Leakage .............................................................................................. 208

8.5 Pressure Problems or Shifts in Retention Time ................................................. 210

8.5.1 Introduction .......................................................................................... 210

8.5.2 Resolving Pressure Pulsation or Shifts In Retention Time .................... 211

8.5.3 Performing Pressure Recalibration ....................................................... 213

8.5.4 Testing the Inline Filter/Static Mixer for Permeability .......................... 214

9 Specifications ................................................................................ 215

9.1 Performance Specifications ............................................................................... 216

9.2 Physical Specifications ....................................................................................... 218

Page 8 Vanquish Pumps (VH-P10-A-02) Operating Manual

Contents

10 Accessories, Consumables and Replacement Parts ...................... 219

10.1 General Information ........................................................................................... 220

10.2 Ship Kit ................................................................................................................ 221

10.3 Optional Accessories ........................................................................................... 222

10.4 Consumables and Replacement Parts ................................................................ 223

11 Appendix ..................................................................................... 227

11.1 Compliance Information ..................................................................................... 228

11.1.1 Declarations of Conformity .................................................................... 228

11.1.2 WEEE Compliance .................................................................................. 229

11.1.3 FCC Compliance ..................................................................................... 229

11.1.4 Manual Release History ......................................................................... 229

11.2 Digital I/O ............................................................................................................ 230

12 Index ........................................................................................... 233

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 9

Contents

Page 10 Vanquish Pumps (VH-P10-A-02) Operating Manual

1 • Using this Manual

1 Using this Manual

This chapter provides information about this manual, the conventions used

throughout the manual, and the reference documentation that is available

in addition to this manual.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 11

1 • Using this Manual

1.1 About this Manual

This manual describes the functional features and operating principle of
your Vanquish™ pump and provides instructions for installation, set up,
start up, shut down, operation, maintenance and troubleshooting.

The layout of this manual is designed to provide quick reference to the
sections of interest to the user. To obtain a full understanding of your
pump, read this manual thoroughly.

This manual also contains safety messages, precautionary statements,
and special notices that can prevent personal injury, damage to the
pump, or loss of data when followed properly.

Note the following:

• The pump configuration may vary; therefore, not all descriptions

necessarily apply to your particular pump.

• If some detail applies to only one model or variant, the model or

variant is identified by name.

• Illustrations in this manual are provided for basic understanding.

They can vary from the actual model of the pump or component.
However, this does not influence the descriptions. No claims can be
derived from the illustrations in this manual.

The descriptions in this manual assume that the pump is installed in the
Vanquish system stack. If this is not the case, additional hardware is
required and must be ordered separately. The information in this
manual applies correspondingly.

Page 12 Vanquish Pumps (VH-P10-A-02) Operating Manual

1.2 Conventions

This section describes the conventions that are used throughout this
manual.

1.2.1 Safety Messages

The safety messages and precautionary statements in this manual
appear as follows:

• Safety messages or precautionary statements that apply to the

• Safety messages or precautionary statements that apply to an entire

1 • Using this Manual

entire manual and all procedures in this manual are grouped in the
Safety chapter.

section or to multiple procedures in a section appear at the
beginning of the section to which they apply.

• Safety messages that apply to only a particular section or procedure

appear in the section or procedure to which they apply. They appear
different from the main flow of text.

Safety messages are often preceded by an alert symbol and/or alert
word. The alert word appears in uppercase letters and in bold type.

Make sure that you understand and follow all safety messages
presented in this manual.

1.2.2 Special Notices and Informational Notes

Special notices and informational notes in this manual appear different
from the main flow of text. They appear in boxes and a note label
identifies them. The label text appears in uppercase letters and in bold
type.

NOTICE Highlights information necessary to prevent damage to the
pump or invalid test results.

TIP Highlights information of general interest or helpful information that
can make a task easier or optimize the performance of the pump.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 13

1 • Using this Manual

1.2.3 Typographical Conventions

These typographical conventions apply to the descriptions in this
manual:

Data Input and Output

• The following appears in bold type:
♦ Input that you enter by the keyboard or that you select with the

mouse

♦ Buttons that you click on the screen

♦ Commands that you enter by the keyboard

♦ Names of, for example, dialog boxes, properties, and parameters

• For brevity, long expressions and paths appear in the condensed

form, for example: Click Start > All Programs > Thermo
Chromeleon 7 > Services Manager > Start Instrument Controller.

References and Messages

• References to additional documentation appear italicized.

• Messages that appear on the screen are identified by quotation

marks.

Viewpoint

If not otherwise stated, the expressions left and right in this manual
always refer to the viewpoint of a person that is facing the pump from
the front.

Particularly Important Words

Particularly important words in the main flow of text appear italicized.

Electronic Manual Version (PDF)

The electronic version (PDF) of the manual contains numerous links that
you can click to go to other locations within the manual. These include:

• Table of contents entries

• Index entries

• Cross-references (in blue text), for example, to sections and figures

Page 14 Vanquish Pumps (VH-P10-A-02) Operating Manual

1.3 Reference Documentation

In addition to this operating manual, other documentation is available
for reference.

Hardware Documentation

Additional hardware documentation includes the following:

• Operating manuals for the other modules of the Vanquish system

A printed version of the manual is shipped with the device.

• Vanquish System Operating Manual

A printed version of the manual is shipped with the Vanquish system
base and solvent rack.

• Instrument Installation Qualification Operating Instructions

1 • Using this Manual

TIP Electronic versions of these manuals are available as PDF (Portable
Document Format) files. To open and read the PDF files, Adobe™
Reader™ or Adobe™ Acrobat™ is required.

Software Documentation

Additional software documentation includes the following:

• Chromeleon™ 7 Help and documents

The Chromeleon 7 Help provides extensive information and
comprehensive reference material for all aspects of the software.
For basic information about device installation and configuration,
refer to the Installation Guide; for specific information about a
certain device, refer to the Instrument Configuration Manager Help.
In Chromeleon 7, devices are called modules.

For information about the main elements of the user interface and
step-by-step guidance through the most important workflows, refer
to the Quick Start Guide.

For a concise overview of the most important workflows, refer to
the Reference Card.

• Chromeleon™ 6.8 Help

The Chromeleon 6.8 Help provides extensive information for all
aspects of the software, including device installation and
configuration.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 15

1 • Using this Manual

Third-Party Documentation

TIP The Chromeleon Help and documents are included in the software
shipment.

Refer also to the user documentation provided by the manufacturers of
third-party components and materials, for example, Safety Data Sheets
(SDSs).

Page 16 Vanquish Pumps (VH-P10-A-02) Operating Manual

2 • Safety

2 Safety

This chapter provides general and specific safety information and informs

about the intended use of the pump.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 17

2 • Safety

2.1 Safety Symbols and Signal Words

2.1.1 Safety Symbols and Signal Words in This Manual

This manual contains safety messages to prevent injury of the persons
using the pump. The safety symbols and signal words in this manual
include the following:

Always be aware of the safety information. Do not proceed until you
have fully understood the information and consider the consequences of
what you are doing.

CAUTION Indicates a hazardous situation that, if not avoided, could
result in minor or moderate injury.

WARNING Indicates a hazardous situation that, if not avoided, could
result in serious injury.

Page 18 Vanquish Pumps (VH-P10-A-02) Operating Manual

2.1.2 Observing this Manual

Observe the following:

• Before installing or operating the pump, read this manual carefully

to be familiar with the pump and this manual. The manual contains
important information with regard to user safety as well as use and
care of the pump.

• Always keep the manual near the pump for quick reference.

• Save this manual and pass it on to any subsequent user.

Read, understand, and comply with all safety messages and
precautionary statements presented in this manual.

2 • Safety

2.1.3 Safety Symbols on the Pump

The table lists the safety symbols that appear on the pump or on labels
affixed to the pump. Follow the safety notices in this manual to prevent
the risk of operator injury or damage to the pump.

Symbol Description

Indicates a potential hazard. Refer to this manual to avoid the risk of
personal injury and/or to prevent damage to the device.

—

Ο

Power supply is on

Power supply is off

Indicates alternating current.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 19

2 • Safety

2.1.4 Rating Plate

The rating plate is present on the pump near the electrical connections.
The rating plate indicates the serial number, part number, module
name, revision number (if any), line and fuse rating, and the
manufacturer's address.

TIP An additional type label on the leak tray of the pump indicates the
module name, serial number, part number, and revision number (if any).
To facilitate device identification have the information from this label
available when communicating with Thermo Fisher Scientific.

Page 20 Vanquish Pumps (VH-P10-A-02) Operating Manual

2.2 Intended Use

The pump is intended to be part of the Vanquish system.

The intended use of the Vanquish system is to analyze mixtures of
compounds in sample solutions. The pump is for use by qualified
personnel and in laboratory environment only.

The pump and Vanquish system are intended for laboratory research
use only. They are not intended for use in diagnostic procedures.

Laboratory Practice

Thermo Fisher Scientific recommends that the laboratory in which the
Vanquish system is used follow best practices for LC analyses. This
includes among others:

2 • Safety

• Using appropriate standards

• Regularly running calibration

• Establishing shelf life limits and following them for all consumables

used with the system

• Running the system according to the laboratory's verified and

validated 'lab developed test' protocol

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 21

2 • Safety

2.3 Safety Precautions

2.3.1 General Safety Information

All users must observe the general safety information presented in this
section and all specific safety messages and precautionary statements
elsewhere in this manual during all phases of installation, operation,
troubleshooting, maintenance, shutdown, and transport of the pump.

If the pump is used in a manner not specified by Thermo Fisher Scientific,
the protection provided by the pump could be impaired. Observe the
following:

• Operate the pump only within its technical specifications.

• Use only the replacement parts and additional components, options,

and peripherals specifically authorized and qualified for the pump by
Thermo Fisher Scientific.

Safety Standard

• Perform only the procedures that are described in this operating

manual and in supporting documents for the pump.
Follow all instructions step by step and use the tools recommended
for the procedure.

• Open the enclosure of the pump and other components only if

specifically instructed to do so in this manual.

• Thermo Fisher Scientific cannot be held liable for any damage,

material or otherwise, resulting from inappropriate or improper use
of the pump. If there is any question regarding appropriate usage,
contact Thermo Fisher Scientific before proceeding.

This device is a Safety Class I instrument (provided with terminal for
protective grounding). The device has been manufactured and tested
according to international safety standards.

Page 22 Vanquish Pumps (VH-P10-A-02) Operating Manual

2.3.2 Qualification of the Personnel

Observe the information below on the proper qualification of the
personnel installing and/or operating the pump.

Installation

Only skilled personnel are permitted to install the pump and to establish
the electrical connections according to the appropriate regulations.
Thermo Fisher Scientific recommends always having service personnel
certified by Thermo Fisher Scientific perform the installation (for brevity,
referred to as Thermo Fisher Scientific service engineer).

If a person other than a Thermo Fisher Scientific service engineer installs
and sets up the module, the installer is responsible for ensuring the
safety of the module and system.

2 • Safety

General Operation

The pump is designed to be operated only by trained and qualified
personnel in a laboratory environment.

All users must know the hazards presented by the pump and the
substances they are using. All users should observe the related Safety
Data Sheets (SDSs).

2.3.3 Personal Protective Equipment

Wear personal protective equipment and follow good laboratory
practice to protect you from hazardous substances. The appropriate
equipment depends on the hazard. For advice on the hazards and the
equipment required for the substances you are using, refer to the
material handling and safety data sheet provided by the vendor.

An eyewash facility and a sink should be available nearby. If any
substance contacts your skin or eyes, wash the affected area and seek
medical attention.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 23

2 • Safety

Protective Clothing

To protect you from chemical splashes, harmful liquids, or other
contamination, put on appropriate protective clothing, such as a lab
coat.

Protective Eyewear

To prevent liquids from striking your eyes, put on appropriate protective
eyewear, such as safety glasses with side shields. If there is a risk of
splashing liquids, put on goggles.

Gloves

To protect you from harmful liquids and avoid personal injury during
maintenance or service, put on appropriate protective gloves.

2.3.4 Electrical Safety Precautions

WARNING— Electric Shock or Damage to the Device

High voltages are present inside the device that could cause an electric
shock or damage to the device.

• Do not make any changes to the electrical or grounding connections.

• If you suspect any kind of electrical damage, disconnect the power

cord and contact Thermo Fisher Scientific Technical Support for
assistance.

• Do not open the housing or remove protective panels unless

specifically instructed to do so in this manual.

• Do not place liquid reservoirs directly upon the device. Liquid might

leak into the device and get into contact with electronic components
causing a short circuit. Instead, place liquid reservoirs in the solvent
rack that is available for the Vanquish system.

Page 24 Vanquish Pumps (VH-P10-A-02) Operating Manual

2.3.5 General Residual Hazards

Pay attention to the following general residual hazards when working
with the pump:

WARNING—Hazardous Substances

Solvents, mobile phases, samples, and reagents might contain toxic,
carcinogenic, mutagenic, infectious, or otherwise harmful substances.
The handling of these substances can pose health and safety risks.

• Be sure that you know the properties of all substances that you are

using. Avoid exposure to harmful substances. If you have any doubt
about a substance, handle the substance as if it is potentially
harmful.

• Wear personal protective equipment as required by the hazard and

follow good laboratory practice.

2 • Safety

• Reduce the volume of substances to the minimum volume required

for sample analysis.

• Do not operate the pump in a potentially flammable environment.

• Avoid accumulation of harmful substances. Make sure that the

installation site is well ventilated.

• Dispose of hazardous waste in an environmentally safe manner that

is consistent with local regulations. Follow a regulated, approved
waste disposal program.

WARNING—Biohazard

Biohazardous material, for example microorganisms, cell cultures,
tissues, body fluids, and other biological agents can transmit infectious
diseases. To avoid infections with these agents:

• Assume that all biological substances are at least potentially

infectious.

• Wear personal protective equipment as required by the hazard and

follow good laboratory practice.

• Dispose of biohazardous waste in an environmentally safe manner

that is consistent with local regulations. Follow a regulated,
approved waste disposal program.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 25

2 • Safety

WARNING—Self-Ignition of Solvents

Solvents with a self-ignition temperature below 150 °C might ignite
when in contact with a hot surface (for example, due to leakage in the
chromatography system). Avoid the use of these solvents.

WARNING—Hazardous Vapors

Mobile phases and samples might contain volatile or flammable
solvents. The handling of these substances can pose health and safety
risks.

• Avoid accumulation of these substances. Make sure that the

installation site is well ventilated.

• Avoid open flames and sparks. Do not operate the pump in the

presence of flammable gases or fumes.

CAUTION—Allergic Reaction

Some capillaries in the Vanquish system are made of MP35N™, a nickel­cobalt based alloy. Individuals with sensitivity to nickel/cobalt may show
an allergic reaction from skin contact.

CAUTION—Sparking due to Electrostatic Discharge

Liquid flowing through capillaries can generate static electricity. This
effect is particularly present with insulating capillaries and non­conductive solvents (for example, pure acetonitrile). Discharge of
electrostatic energy might lead to sparking, which could constitute a fire
hazard. Prevent the generation of static electricity near the
chromatography system.

2.3.6 In Case of Emergency

WARNING—Safety Hazard

In case of emergency, disconnect the pump from the power line.

Page 26 Vanquish Pumps (VH-P10-A-02) Operating Manual

2.4 Solvent and Additive Information

To protect optimal functionality of the Vanquish system, observe these
recommendations on the use of solvents and additives:

• The system must be used with reversed-phase (RP) compatible

solvents and additives only.

• Use only solvents and additives that are compatible with all parts in

the flow path.
For details about the materials that are used in the flow path of the
pump, see the Specifications chapter. For information about the
materials that are used in the flow path of the other modules in the
Vanquish system, refer to the Specifications chapter in the
Operating Manual for the module.

Observe the general guidelines and recommendations on the use of
solvents and additives in the chromatography system (see

section 6.5, page 89).

2 • Safety

• Allowed ranges and concentrations (standard system configuration):

♦ pH range: 2-12

♦ Chloride concentration: 1 mol/L or less

• Observe the following:

♦ pH values higher than 9.5 with optical detectors

Avoid using mobile phases with a pH value higher than 9.5
together with optical detectors. This can impair the functionality
and optical performance of the detector flow cell.

♦ pH values of 2 or less or high chloride concentration

With pH values of 2 or less or high chloride concentration, the
application time should be as short as possible. Flush the system
thoroughly after these applications.

♦ Mobile phases containing ammonium hydroxide

In rare cases, a shortened lifetime of reversed-phase (UHMW­PE) piston seals has been observed with high pH, ammonium
hydroxide containing mobile phases and prolonged exposure.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 27

2 • Safety

NOTICE If the system flow path includes a Vanquish charged aerosol
detector, observe the specific solvent and additive information for
this detector. Refer to the operating manual for the Vanquish
charged aerosol detector.

• Follow any specific recommendations presented in other sections of

this manual. Refer also to the operating manuals for all modules in
the Vanquish system. They may provide additional guidelines and

information.

Page 28 Vanquish Pumps (VH-P10-A-02) Operating Manual

2.5 Compliance Information

Thermo Fisher Scientific performs complete testing and evaluation of its
products to ensure full compliance with applicable domestic and
international regulations. When the device is delivered to you, it meets
all pertinent electromagnetic compatibility (EMC) and safety standards
as described in this manual. For details, see section 11.1, page 228.

Changes that you make to the device may void compliance with one or
more of these EMC and safety standards. Changes to the device include
replacing a part or adding components, options, or peripherals not
specifically authorized and qualified for the product by Thermo Fisher
Scientific. To ensure continued compliance with EMC and safety
standards, replacement parts and additional components, options, and
peripherals must be ordered from Thermo Fisher Scientific or one of its
authorized representatives.

2 • Safety

The device has been shipped from the manufacturing site in a safe
condition.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 29

2 • Safety

Page 30 Vanquish Pumps (VH-P10-A-02) Operating Manual

3 • Pump Overview

3 Pump Overview

This chapter introduces you to the pump and the main components.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 31

3 • Pump Overview

3.1 Pump Features

The pump includes the following main features:

• Two-channel pump with each channel based on a dual-piston in-

parallel design

• Independent drive for each piston for high accuracy and increased

flexibility

• Advanced Thermal Effect Compensation (ATEC) and electronic

compressibility compensation (SmartFlow™) for precise and virtually
pulse-free flow

• Active piston seal wash system for high lifetime of pistons and piston

seals
The piston seal wash system keeps the pistons wet and rinses the
piston seals automatically. This prevents crystallization and particle
deposits on the piston surfaces and seals, which may damage the
components.

• Solvent selector "2 from 6" for increased flexibility

• Purge unit for easy and automatic purging

• Built-in vacuum degasser for enhanced flow stability and best

detector sensitivity

• Easy access to the user-serviceable components and tool-free

maintenance procedures for fast and reliable maintenance

• Easy maintenance because the pump remains in the Vanquish

system stack

For specifications, see section 9.1 Performance Specifications, page 216.

Page 32 Vanquish Pumps (VH-P10-A-02) Operating Manual

3.2 Operating Principle

The pump can generate pressures up to 151 MPa with ultra-low
pulsation. The pump is based on a two-channel, dual-piston design with
electronic compressibility compensation.

The picture illustrates how the pump operates:

3 • Pump Overview

Figure 1: Operating principle

No. Description

1 Solvent selector (pump inlet)

2 Solvent degasser (vacuum degasser)

3 Pump head, left (two pistons, parallel)

4 Y-connector, left

5 Pump head, right (two pistons, parallel)

6 Y-connector, right

7 Purge unit

8 System pressure sensor

9 Inline filter (static filter)

10 Pump outlet (outlet of the inline filter)

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 33

3 • Pump Overview

Each pump head comprises two cylinders that are connected in parallel.
Thus, the solvent passes only one cylinder when the pump is delivering.
To achieve continuous delivery, one cylinder is refilled while the other
cylinder is delivering at the selected flow rate.

The pump uses independent piston drives and highly precise position
and pressure sensors. This leads to high accuracy, increased reliability,
and ultra-low pulsation when delivering compressible liquids. The flow
rate at atmospheric pressure is always kept constant.

Two 3-port solvent selectors allow forming binary gradients from three
solvents per channel. The solvent to be used for each channel is selected
in the user interface.

The vacuum degasser enhances the flow stability and allows best
detector sensitivity by continuously removing air that may be trapped in
the solvents.

The solvent flow is through the pump head, from the inlet assembly to
the pump cylinders, and on to a Y-connector. The Y-connector combines
the solvent flows from the pump head cylinders.

From each Y-connector, the solvent stream is directed to the purge unit,
which combines the two solvent streams, and on to the inline filter.

Page 34 Vanquish Pumps (VH-P10-A-02) Operating Manual

3.3 Interior Components

The user-accessible components of the pump are located directly behind
the front doors:

3 • Pump Overview

Figure 2: Interior view

No. Description

1 Keypad with buttons for pump control

2 Pump head, left

3 Tubing clips for routing solvent lines to the pump heads

4 Pump head, right

5 Leak sensor

6 Type label, indicating the module name, serial number, part number, and

revision number (if any)

7 Drain port

8 Seal wash detector (drop detector)

9 Vacuum degasser (solvent B) (partly hidden by seal wash detector)

10 Solvent selector (solvent B)

11 Vacuum degasser (solvent A)

12 Solvent selector (solvent A)

13 Seal wash pump

14 Tubing chase with tubing guides

15 Inline filter (static filter)

16 Y-connector, right

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 35

3 • Pump Overview

No. Description

17 Purge unit

18 Y-connector, left

19 System pressure sensor

Page 36 Vanquish Pumps (VH-P10-A-02) Operating Manual

3.4 Piston Seal Wash System

To keep the pistons wet and rinse the piston seals, the pump has an
active piston seal wash system (seal wash system). Piston seal washing
prolongs the lifetime of the pistons and seals by preventing
crystallization and particle deposits on the piston surfaces and seals,
which may otherwise damage the pistons and seals.

The picture shows the flow path through the seal wash system.

3 • Pump Overview

Figure 3: Flow path through the seal wash system

No. Description

1 Supply line for seal wash liquid (from autosampler)

2 Seal wash pump (peristaltic pump)

3 Peristaltic tubing (PharMed™ tubing)

4+5 Pump heads

6 Seal wash detector (drop detector)

7 To waste

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 37

3 • Pump Overview

3.5 Purge Unit

The purge unit combines the solvent streams from the pump heads,
connects the system pressure sensor to the flow system, and allows easy
and automatic purging. The pump is shipped from the manufacturing
site with the capillaries and tubing installed to the purge unit.

Figure 4: Connections on purge unit

No. Description

1 Capillary from left Y-connector

2 Capillary to system pressure sensor (long capillary)

3 Capillary to inline filter

4 Capillary from right Y-connector

5 Waste tubing (from purge unit to drain port)

6 Capillary from system pressure sensor (short capillary)

Page 38 Vanquish Pumps (VH-P10-A-02) Operating Manual

3.6 Leak Detection

Leaks are a potential safety issue. The leak sensor inside the pump
monitors the pump for liquid leaks from the flow connections. The liquid
is collected in the leak tray and guided to the drain port. From the drain
port, the liquid is discharged to waste through the drain system of the
Vanquish system.

When the leak sensor detects leakage, the status indicators change to
red and beeping starts to alert you. Follow the instructions in this
manual to find and eliminate the source for the leakage.

If leakage is not resolved after 3 minutes, the pump stops the flow.

3 • Pump Overview

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 39

3 • Pump Overview

3.7 Operation

The pump is designed to be operated from a computer configured with
the Chromeleon Chromatography Data System (CDS). The Chromeleon
software provides complete instrument control, data acquisition, and
data management.

For a basic description of instrument control and automated sample
analysis with the Chromeleon software, refer to the Vanquish System
Operating Manual. Details on control and operation of the pump are
available in the Chromeleon Help.

TIP The pump can be operated also with other data systems, such as
Xcalibur™. In this case, installation of additional software is required in
addition to the data system software. For details, contact the Thermo
Fisher Scientific sales organization.

A keypad is available inside the pump, allowing you to perform certain
basic functions directly from the pump.

Page 40 Vanquish Pumps (VH-P10-A-02) Operating Manual

4 • Unpacking

4 Unpacking

This chapter provides information for unpacking the pump and informs you

about the scope of delivery.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 41

4 • Unpacking

4.1 Unpacking

Damaged Packaging, Defective on Arrival

Unpacking the Pump

Inspect the shipping container for signs of external damage and, after
unpacking, inspect the pump for any signs of mechanical damage that
might have occurred during shipment.

If you suspect that the pump may have been damaged during shipment,
immediately notify the incoming carrier and Thermo Fisher Scientific
about the damage. Shipping insurance will compensate for the damage
only if reported immediately.

Tools required

Follow these steps

CAUTION—Heavy Load, Bulky Device

The pump is too heavy or bulky for one person alone to handle safely. To
avoid personal injury or damage to the pump, observe the following
guidelines:

• Physical handling of the pump, including lifting or moving, requires a

team effort of two persons.

• Use the carrying handles that were shipped with the pump to move

or transport the pump. Never move or lift the pump by the front
doors. This will damage the doors or the pump.

Screwdriver, Torx™ T20

1. Place the shipping container on the floor and open it.

2. Remove the ship kit from the shipping container.

Page 42 Vanquish Pumps (VH-P10-A-02) Operating Manual

4 • Unpacking

3. Remove the pump from the shipping container: Grasp the pump by
the carrying handles. Slowly and carefully, lift the pump out of the
shipping container.

Figure 5: Carrying handles on the pump

No. Component

1 Carrying handles

2 Attachment screw (one on each carrying handle)

4. Place the pump on a stable surface.

5. If applicable
Remove any additional packing material. Leave any protective films
attached to the surfaces of the pump until it is properly positioned in
the system stack.

6. Transport the pump by the carrying handles to the installation site, if
it is not already there, and place it in the system stack (see the
System Arrangement section in this operating manual).

7. On each carrying handle, loosen the attachment screw until the
carrying handle is moveable in the rail. Do not remove the screws
from the carrying handles completely.

8. Slide off the carrying handles from the rails towards the rear of the
pump.

Figure 6: Sliding off the carrying handle from the left rail

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 43

4 • Unpacking

TIP Keep the shipping container, the carrying handles with the
attachment screws, and all packing material. These items will be
needed if the pump is transported to a new location or shipped.

9. Some surfaces including the doors of the pump are covered by a
protective film during shipment. Remove the protective film from all
surfaces as applicable.

Page 44 Vanquish Pumps (VH-P10-A-02) Operating Manual

4.2 Scope of Delivery

The following items are included in the delivery:

• Pump

• Ship Kit

For details about the kit content, see section 10.2 Ship Kit, page 221.

• Operating manual

• Power cord

4 • Unpacking

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 45

4 • Unpacking

Page 46 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

5 Installation

This chapter specifies the requirements for the installation site and

describes how to set up, install, and configure the pump in the Vanquish

system and in the chromatography software.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 47

5 • Installation

5.1 Safety Guidelines for Installation

Pay attention to the following safety guidelines:

Observe all warning messages and precautionary statements presented
in section 2.3 Safety Precautions (see page 22).

CAUTION—Heavy Load, Bulky Device

The pump is too heavy or bulky for one person alone to handle safely. To
avoid personal injury or damage to the pump, observe the following
guidelines:

• Physical handling of the pump, including lifting or moving, requires a

team effort of two persons.

• Use the carrying handles that were shipped with the pump to move

or transport the pump. Never move or lift the pump by the front
doors. This will damage the doors or the pump.

Page 48 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.2 Installing the Pump

The Vanquish system is installed and set up by a Thermo Fisher Scientific
service engineer, including all modules and options or parts shipped with
them. The service engineer checks that the installation is correct and
that the Vanquish system and modules operate as specified. The
engineer also demonstrates the basic operation and main features.

If personnel other than a Thermo Fisher Scientific service engineer
installs the pump, follow the steps below.

NOTICE The pump is part of the Vanquish system. Therefore, follow the
order for installing the system modules as described in the Vanquish
System Operating Manual.

1. Pay attention to the safety guidelines and observe all site
requirements.
For the safety guidelines when installing the pump, see section 5.1,

page 48. For the site requirements, see section 5.3, page 51.

5 • Installation

2. Set up the pump hardware. See section 5.5, page 54.

3. Set up the flow connections. See section 5.6, page 59.

4. Turn on the pump. See section 5.7, page 78.

TIP Before turning on the power to a Vanquish system module for the
first time, verify that the chromatography software is installed on the
data system computer. When the power is turned on, the required USB
drivers are automatically found and the Windows™ operating system
can detect the device.

5. Set up the pump in the software. See section 5.8, page 79.

6. Recommended:
Perform Instrument Installation Qualification.

In Chromeleon, a wizard is available to guide you through the
qualification process:

♦ On the Chromeleon 7 Console: Click Tools > Instrument

Qualification > Installation Qualification.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 49

5 • Installation

♦ In the Chromeleon 6.8 Browser: Click Qualification >

Instruments IQ.

Follow the instructions in the Instruments Installation Qualification
Operating Instructions. The manual provides information about the

required materials and detailed instructions.

NOTICE If the pump is operated with another data system, refer to the
documentation for the software that you are using and/or perform the
qualification manually.
The Instruments Installation Qualification Operating Instructions provide
information about the parameters to be adapted and the required
settings.

7. Recommended:
Perform Operational Qualification.

The qualification kit includes all materials required for the
qualification and detailed instructions.

Moving the Pump after Installation

If you have to move the pump after it has been set up and installed in
the Vanquish system, prepare the pump for transport and move it to the
new location. Follow the instructions in section 7.16 Transporting or

Shipping the Pump, page 186.

Page 50 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.3 Site Requirements

The operating environment is important to ensure optimal performance
of the pump. This section provides important requirements for the
installation site. Note the following:

• Operate the pump only under appropriate laboratory conditions.

• The pump is intended to be part of the Vanquish system. Observe

the site requirements for the Vanquish system as stated in the
Vanquish System Operating Manual.

• For specifications, see the Specifications section in this operating

manual and the Operating Manuals for the other modules in the
Vanquish system.

Power Considerations

5 • Installation

The power supply of the device has wide-ranging capability, accepting
any line voltage in the range specified for the device.

CAUTION—Electric Shock or Damage to the Device

• Connecting the device to a line voltage higher or lower than

specified could result in personal injury or damage to the device.
Therefore, connect the device to the specified line voltage only.

• Never connect the device to a power socket that is shared with other

equipment (for example, multiple sockets).

• Do not use extension cords.

• After the power to the device is turned off, the device is still

energized as long as the power cord is connected. Repair work on
the device while the device is connected to power could lead to
personal injury. Therefore, always unplug the power cord before
starting repair work inside the device. If you were instructed to
remove any covers or panels, do not connect the power cord to the
device while the cover or panels are removed.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 51

5 • Installation

Power Cord

The power cords are designed to match the wall socket requirements of
the country in which they are used. The end of the power cords that
plugs into the power socket on the device is identical for all power cords.
The end of the power cords that plugs into the wall socket is different.

WARNING—Electric Shock or Damage to the Device

• Never use a power cord other than the power cords provided by

Thermo Fisher Scientific for the device.

• Only use a power cord that is designed for the country in which you

use the device.

• Do not use extension cords.

• Never plug the power cord to a power socket that is shared with

other equipment (for example, multiple sockets).

Condensation

• Operate the device only from a power outlet that has a protective

ground connection.

• In case of emergency, it must be possible to reach the power cord

easily at any time to disconnect the device from the power line.

WARNING—Electric Shock or Damage to a Product

Misuse of the power cords could cause personal injury or damage the
instrument. Use the power cords provided by Thermo Fisher Scientific
only for the purpose for which they are intended. Do not use them for
any other purpose, for example, for connecting other instruments.

NOTICE Condensation in the device can damage the electronics.
Therefore, when using, shipping, or storing the device, avoid or minimize
conditions that can lead to a build-up of condensation in the device. For
example, avoid significant or fast changes in environmental conditions.
If you suspect that condensation is present, allow the device to warm up
to room temperature. This may take several hours. Wait until the
condensation is gone completely before connecting the pump to the
power line.

Page 52 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.4 Accessing the Interior Components

To access the interior components in the pump, open the front doors. To
allow easy access from the front, the user-accessible components and
flow connections in the pump are located directly behind the doors.

5 • Installation

Figure 7: Opening the pump doors

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 53

5 • Installation

5.5 Setting Up the Hardware

This section describes how to set up the hardware and provides
information about the pump connectors and cables.

5.5.1 System Arrangement

The pump is part of the Vanquish system. The system modules are
typically arranged in a system stack, with the arrangement depending on
the system configuration.

Figure 8: Vanquish system, standard configuration (example)

No. Description

1 Solvent Rack

2 Detector

3 Autosampler

Page 54 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

No. Description

4 Pump

5 System Base

6 Column Compartment

For instructions on how to set up the system stack, refer to the Vanquish

System Operating Manual.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 55

5 • Installation

5.5.2 Connecting the Pump

Pump Connectors

The following connectors are provided on the pump:

Figure 9: Electrical and device connectors on the pump

No. Description

1 Rating plate, indicating the serial number, part number, module name, revision

number (if any), line and fuse rating, and the manufacturer's address

2

Main power switch (on/off control)

3

Fuse holder

4

Power-inlet connector

5 System Interlink port

Allows power on/off control for the pump from the Vanquish system base and
device communication

Page 56 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

No. Description

6 Digital I/O ports (Dig I/O)

Allow exchange of digital signals with external instruments
Each digital I/O port provides one input, one relay output, and one bidirectional
input/output. For connection and pin assignment information, see section 11.2,

page 230.

7 USB hub ("A" type connector)

Allows connection to other modules in the Vanquish system

8 USB (Universal Serial Bus) port ("B" type connector)

Allows connection to other modules in the Vanquish system or the computer
on which the data management system is installed, such as the Chromeleon
software

TIP Thermo Fisher Scientific recommends using the USB ports only as
described above. If the USB ports are used for any other purpose,
Thermo Fisher Scientific cannot ensure proper functionality.

Connecting the Pump

NOTICE

• Never use defective communication cables. If you suspect that a

cable is defective, replace the cable.

• To ensure trouble-free operation, use only the cables provided by

Thermo Fisher Scientific for connecting the pump.

1. Place the pump in the system as required by the system
configuration. For details, refer to the Vanquish System Operating
Manual.

2. Connect the required interface cables to the pump.
For information about how to connect the pump to other modules in
the Vanquish system or to the chromatography data system
computer, refer to the Vanquish System Operating Manual.

3. Verify that the power switch on the pump is set to OFF.

4. Connect the power cord to the power-inlet connector on the pump.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 57

5 • Installation

NOTICE Before connecting the pump to the power line, be sure that no
condensation is present in the device. Condensation in the device can
damage the electronics. If you suspect that condensation is present,
allow the device to warm up to room temperature. This may take
several hours. Wait until the condensation is completely gone before
proceeding.

5. Connect the free end of the power cord to an appropriate power
source.

Page 58 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.6 Setting Up the Flow Connections

5.6.1 General Information and Guidelines

The pump is shipped from the manufacturing site with the flow
connections installed between the interior components.

This section describes how to set up the flow connections to and from
the pump and additional flow connections if required.

When setting up flow connections, follow these rules and
recommendations:

Flow connections can be filled with hazardous substances. Observe the
warning messages and precautionary statements presented in

section 2.3 Safety Precautions (see page 22).

5 • Installation

• Dirty components can contaminate the chromatography system.

Contamination leads to poor performance of the modules and entire
system or can even cause damage to the modules and system.
Therefore:

♦ Always wear appropriate gloves.

♦ Place the components only on a clean, lint-free surface.

♦ Keep your tools clean.

♦ Use only lint-free cloth for cleaning.

• For installation instructions and handling guidelines for capillaries,

tubing, and fittings, see section 5.6.3 Connecting Fittings, Capillaries,

and Tubing, page 62.

To set up the additional flow connections and complete the installation,
follow these steps:

TIP Components or connections in the flow path to other system
modules may be closed with plugs to protect the component or
connection during transport. When you remove the plugs to connect the
pump in the system, keep the plugs. You may need them to close the
connections again, for example, for future transport.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 59

5 • Installation

1. Connect the pump to the drain system (see section 5.6.4, page 64).

2. Connect the solvent lines (see section 5.6.5, page65).

3. Set up the seal wash system (see section 5.6.6, page 69).

4. Connect the pump to the autosampler (see section 5.6.7, page 77).

NOTICE When you install devices or components to the system, always
flush them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the Vanquish
System Operating Manual.

5.6.2 Guiding Capillaries and Tubing Through the System

Flow connections between the modules of the Vanquish system are
guided through either the tubing chase in the devices or the guide holes
or capillary clips of the devices.

Tubing Chase, Tubing Guide, Tubing Bracket

To guide certain tubes and lines (solvent tubing, wash liquid tubing,
detector waste line) from the top module to the bottom module in the
Vanquish system stack, the stackable modules have a tubing chase on
the inside right.

The tubing chase provides four tubing guides. Each guide can hold up to
three tubes or lines.

In each module, push the tube (or line) into the appropriate guide (see
picture).

Page 60 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

Figure 10: Tubing chase with tubing guides (left: view from inside, right:
view from top)

No. Use for

1 Solvent tubing (up to three solvent lines)

2 Solvent tubing (up to three solvent lines)

3 Wash liquid tubing (seal wash, autosampler needle wash)

4 Detector waste line

Tubing brackets are available for holding the tubing in place. Slip the
bracket side onto the drain pipe.

Figure 11: Tubing bracket (left), tubing bracket installed (right)

Guide Holes and Capillary Clips

Guide holes and capillary clips are provided at specific positions on the
system modules. Route flow connections from one module to the next
module in the Vanquish system through the appropriate guide hole or
capillary clip when instructed to do so in the manual.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 61

5 • Installation

5.6.3 Connecting Fittings, Capillaries, and Tubing

This section provides information about how to connect and handle
capillaries, fittings, and tubing.

5.6.3.1 General Guidelines

When connecting capillaries and tubing, follow these general
recommendations:

• Use only the capillaries and tubing (for example, solvent lines or

waste tubing) that are shipped with the pump or additional or spare
capillaries and tubing as recommended by Thermo Fisher Scientific.

• The connectors must be free from contaminants. Even minute

particles may cause damage to the system or lead to invalid test
results.

• Do not install capillaries or tubes that are stressed, nicked, kinked, or

otherwise damaged.

• Install capillaries and fittings only at the positions for which they are

intended.

5.6.3.2 Connecting Viper Capillaries

This section describes how to connect Viper™ capillaries. All Viper flow
connections in the Vanquish system are designed to be finger-tight.

To connect Viper capillaries with knurl, follow these steps:

NOTICE

• Tighten or loosen Viper capillaries only with your fingers. Do not use

tools other than the knurl that comes with the capillary.

• To avoid damage to the capillary or connection, tighten and loosen

the Viper connections only when the system pressure is down to
zero.

Page 62 Vanquish Pumps (VH-P10-A-02) Operating Manual

Figure 12: Viper fitting with knurl

No. Description

1 Knurl

2 Capillary

5 • Installation

3 Slot

1. Insert the Viper capillary into the connection port.

2. Tighten the connection by the knurl.

TIP Note the slot in the knurl. For narrow connections, you can easily
remove the knurls from neighboring capillaries through this slot and
attach them again later.

3. If the connection leaks:

a) Tighten the connection a little more.

b) If leakage continues, remove the capillary.

c) Clean the capillary ends carefully by using a lint-free tissue

wetted with isopropanol.

d) Reinstall the capillary.

e) If the connection continues to leak, install a new Viper capillary.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 63

5 • Installation

5.6.4 Guiding Liquids to Waste

The following liquids leave the pump through the outlets at the bottom
right of the leak tray:

• Leaking liquid from the leak tray

Leaking liquid collects in the leak tray and flows off through the
drain port outlet into the drain system of the Vanquish system.

• Waste liquid from the purge unit

Waste liquid from the purge unit flows off through the waste outlet
port into the drain system of the Vanquish system. To avoid false
leak alarms, verify that the waste line sits properly in the waste
outlet port. If it does not, liquid from the purge unit may accumulate
in the drain port and activate the leak sensor.

Figure 13: Outlets for guiding liquids to waste (view from top)

No. Description

1 Waste line from purge unit: routing path to outlet

2 Drain port, with

2a Drain port wall

2b Groove in drain port wall for routing the waste line to the waste outlet

port

2c Drain port outlet (to Vanquish drain system)

3 Waste outlet port (to Vanquish drain system)

4 Leak sensor

For information about how the liquid is discharged to waste through the
Vanquish drain system, refer to the Vanquish System Operating Manual.

Page 64 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.6.5 Connecting the Solvent Lines

The pump is shipped from the manufacturing site with the solvent lines
installed between the components in the pump. To complete the setup,
install the solvent lines between the pump inlet ports and the solvent
reservoirs.

The flow path determines the pump inlet (component at which the
solvent enters the pump) and the solvent flow through the pump. With
the binary pump, the pump inlets are the solvent selectors. The
operating principle illustrates the solvent flow through the pump (see

section 3.2, page 33).

Parts and tools required

• Solvent reservoir assemblies, including reservoir, reservoir cap, cap

plugs, and retaining guide for the solvent line

5 • Installation

• Solvent lines

• Solvent line filters

• Optional to shut off the solvent flow through the system at the

solvent reservoir

♦ Shut off valve, kit containing one shut off valve and fitting

connections

♦ Tubing cutter

If you want to install shut off valves after initial setup or operation of
the pump, follow the steps in section 6.9.3 Installing Solvent Shut

Off Valves, page 109.

TIP To avoid the formation of gas bubbles in the solvent reservoirs, place
the reservoirs in the system stack on the same level or higher than the
pump. The setup description below assumes that the reservoirs are
placed in the solvent rack.

Preparation

• Assemble the solvent line filter, wearing appropriate clean gloves:

a) Place the frit in the filter holder (bottom part).

b) Make sure that the frit is in a level position.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 65

5 • Installation

Follow these steps

c) Screw the filter top to the filter bottom.

No. Description

1 Solvent line filter, bottom

2 Filter frit

3 Solvent line filter, top

Figure 14: Assembling the solvent line filter

• On the pump inlet, remove the plug that protects the inlet during

shipment, if applicable.

1. Connect each solvent line to the pump inlet port for which it is
intended (see the labeling on the solvent lines and in the pump).

TIP For easy access to the lower degas chambers, remove the seal wash
detector assembly (funnel and detector). Grasp and pull the assembly
upward by the funnel. The detector including the tubing and cable can
remain connected to the funnel.

No. Description

1 Funnel, seal wash detector

2 Seal wash detector

3 Detector cable

4 Seal wash line

Figure 15: Removing the seal wash detector assembly

2. Route the solvent lines from the pump inlet to the solvent rack,
through the tubing guides in the pump and all modules above the
pump in the system stack. Observe the routing guidelines in the
Guiding Capillaries and Tubing Through the System section in this
manual.

Page 66 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

3. Feed the solvent lines through the guide hole in the solvent rack and
fix them in the appropriate tubing guides in the solvent rack.

Figure 16: Guide hole and tubing guides in the solvent rack

No. Description

A Guide hole (solvent rack)

1 Solvent lines (up to three solvent lines)

2 Solvent lines (up to three solvent lines)

3+4 Not to be used for solvent lines; reserved for other tubing

4. Prepare the solvent lines:

a) Feed each solvent line through a retaining guide.

b) Feed the solvent line through an open hole in a cap for a solvent

reservoir. The retaining guide keeps the solvent line in place in
the reservoir.

c) Close any open holes in the reservoir caps with the cap plugs.

No. Description

1 Solvent line

2 Retaining guide

3 Reservoir cap

4 Cap plugs

5 Solvent line filter

Figure 17: Preparing the solvent line and reservoir cap

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 67

5 • Installation

5. Place the solvent reservoirs in the solvent rack. Position the solvent
lines straight in the tubing guides.

6. Slide a filter holder onto each solvent line.

7. Fill the solvent reservoirs with solvent, observing the guidelines and
recommendations in the Use of Solvents and Additives section in this
manual.
Before using a solvent reservoir for the first time, rinse the
reservoirs thoroughly. Use a high-purity solvent for rinsing.

8. Tighten the reservoir caps hand-tight. Press the retaining guide into
the hole in the reservoir cap to ensure that the tubing is kept in
place in the cap.

9. Optional – Required only if you want to install a shut off valve

a) Cut the solvent line near the reservoir cap with a tubing cutter.

Make sure that the cut is at a right angle to the length of the
line.

b) Onto the free end of each solvent line, slide a fitting and a

ferrule. Mind the correct orientation of the fitting and ferrule
(see picture).

c) Tighten the solvent lines to the shut off valve.

Figure 18: Installing a shut off valve in a solvent line

No. Description

1 Solvent line

2 Fitting

3 Ferrule

4 Shut off valve

Page 68 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

No. Description

1 Shut off valve

2 Retaining guide

Figure 19: Solvent line with shut off valve installed

10. Check the solvent lines across the entire flow path. Make sure that
the lines are not bent, pinched, or squeezed at any point in the flow
path.

5.6.6 Setting Up the Seal Wash System

The flow path of the seal wash system passes through the metering
device head in the autosampler and the pump heads in the pump. The
seal wash system comprises the following parts:

• In the pump: Seal wash pump (peristaltic pump), seal wash lines,

and seal wash detector (drop detector)

• In the autosampler: Seal wash lines

• Reservoir for the seal wash liquid

Both, the autosampler and pump are shipped with the seal wash
components installed and the lines connected within each of the two
modules.

To complete the setup, interconnect the seal wash paths of the
modules, connect and fill the seal wash reservoir, and flush the seal
wash system. To complete the setup, follow the steps in section 5.6.6.2,

page 70.

5.6.6.1 Choosing the Seal Wash Liquid

Use 75% isopropanol in water and 0.1% formic acid (HPLC-grade;
preparation by volume, for example, 75 mL isopropanol + 25 mL water +

0.1 mL formic acid).

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 69

5 • Installation

5.6.6.2 Setting Up the Seal Wash System

Parts and tools required

• Seal wash reservoir assembly, including reservoir, reservoir cap, cap

plugs, and retaining guide for the seal wash line

• Seal wash line (silicone tubing) for connection to the seal wash

pump; the line is pre-installed to the seal wash outlet of the
metering device head

• Seal wash line (silicone tubing) for connection to the seal wash

reservoir

TIP To avoid the formation of gas bubbles in the seal wash reservoir,
place the reservoir in the system stack on the same level or higher than
the autosampler. The setup description below assumes that the
reservoir is placed in the solvent rack.

Page 70 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

Figure 20: Seal wash system in the Vanquish system

No. Description

1 Seal wash reservoir

2 Seal wash lines in the autosampler (thick tubing):

2a Wash line from reservoir to inlet port

2b Seal wash inlet port (right tubing connector)

2c Wash line from inlet port to metering device head

2d Wash line to seal wash pump

3 Metering device head

4 Seal wash pump (peristaltic pump)

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 71

5 • Installation

Follow these steps

No. Description

5 Seal wash lines in the pump (thin tubing):

5a Wash lines from seal wash pump to left pump head

5b Wash line from left pump head to right pump head

5c Wash line from right pump head to seal wash detector

6 Seal wash detector (drop detector)

1. In the pump, engage the peristaltic tubing (PharMed™ tubing) in the
seal wash pump.

Figure 21: Seal wash pump

No. Description

1 Pump lever

2 Pump rotor

3 Tubing holder (peristaltic tubing)

4 Tubing connectors

5 Seal wash line from autosampler

6 Peristaltic tubing

7 Seal wash tubing to pump head

a) Press down the lever on the seal wash pump and hold it in that

position with one hand.

Page 72 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

b) With your other hand, insert the tubing between the lever and

the rotor and wind it around the rotor.

c) Release the lever.

d) Check that the peristaltic tubing is properly inserted in the

tubing holders. If it is not, push the tubing into the holders.
Make sure that the tubing is not pinched or squeezed in the
holders.

e) Check that the right tubing connector rests on the tubing holder.

If it does not, push the tubing connector onto the holder.

2. In the autosampler, the seal wash line to the pump and the seal
wash inlet port (right tubing connector) are interconnected.
Disconnect the seal wash line (clear silicone tubing) from the seal
wash inlet port (right tubing connector).

No. Description

1 Seal wash inlet port

(right tubing connector)

1a Seal wash line to reservoir

2 Seal wash outlet on the

metering device head

2a Seal wash line to pump

Figure 22: Seal wash connections in the autosampler

3. Route the seal wash line from the seal wash outlet of the metering
device through the tubing guides to the peristaltic tubing in the
pump.

4. In the pump, connect the seal wash line to the tubing connector on
the free end of the peristaltic tubing.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 73

5 • Installation

5. Locate the seal wash line for connection from the autosampler to
the seal wash reservoir.

6. Connect the seal wash line to the seal wash inlet port (right tubing
connector) on the autosampler.

7. Route the seal wash line from the autosampler to the solvent rack
through the tubing guides in the autosampler and all modules above
the autosampler in the system stack.

8. Route the seal wash line through the guide hole in the solvent rack.

Figure 23: Guide hole and tubing guides in the solvent rack

No. Description

A Guide hole

1+2 Not to be used for wash liquid line; reserved for other tubing

3 Tubing guide for wash liquid line

4 Not to be used for wash liquid line; reserved for other tubing

9. Prepare the seal wash line:

a) Feed the seal wash line through the retaining guide.

b) Feed the seal wash line through an open hole in the cap of the

seal wash reservoir. The retaining guide keeps the tubing in
place in the reservoir.

c) Close any open holes in the reservoir cap with cap plugs.

Page 74 Vanquish Pumps (VH-P10-A-02) Operating Manual

5 • Installation

No. Description

1 Seal wash line

2 Retaining guide

3 Reservoir cap

4 Cap plugs

Figure 24: Preparing the seal wash line

10. Fill the seal wash reservoir with seal wash liquid. Mind the
requirements outlined in the previous section.
Before using a seal wash reservoir for the first time, rinse the
reservoir thoroughly. Use a high-purity solvent for rinsing.

11. Tighten the reservoir cap hand-tight. Press the retaining guide into
the hole in the reservoir cap to ensure that the tubing is kept in
place in the cap.

12. Place the seal wash reservoir in the solvent rack. Position the seal
wash lines straight in the tubing guides.

13. Check the seal wash lines across the entire flow path. Make sure
that the lines are not bent, pinched, or squeezed at any point in the
flow path.

14. Flush the seal wash system (see the next section).

5.6.6.3 Flushing the Seal Wash System

To flush the seal wash system, select one of the following alternatives:

• Turn on the pump.

Whenever the pump is turned on, the pump starts operation with a
seal wash cycle.

• Flush the seal wash system from Chromeleon.

• Flush the seal wash system from hand.

Select this alternative to flush the seal wash system when the pump
is powered off.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 75

5 • Installation

Flushing the Seal Wash System from Chromeleon

Flushing the Seal Wash System from Hand

To flush the seal wash system from Chromeleon, start a seal wash cycle
by setting the seal wash pump to Active mode (SealWashPump =
Active).

During the seal wash cycle, the seal wash system is automatically filled
with the seal wash liquid. It will take 5 minutes for the liquid to travel
through the entire flow path.

To flush the seal wash system from hand, follow these steps:

1. Disconnect the tubing from the seal wash detector.

No. Description

1 Seal wash detector

2 Tubing connection port

(port labeled in)

Figure 25: Seal wash detector

2. Insert a syringe into the open end of the tubing.

3. To allow the liquid to travel easily through the seal wash system,
press down the lever of the seal wash pump and hold it in that
position with one hand.

4. Pull out the syringe plunger to draw seal wash liquid into the tubing.

5. Release the lever of the seal wash pump when the liquid reaches the
syringe.

6. Remove the syringe and reconnect the tubing to the connection port
on the seal wash detector.
Mind the port labeling. To ensure correct functioning of the seal
wash detector, the tubing needs to be connected to the port labeled
in.

Page 76 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.6.7 Connecting the Pump and Autosampler

Parts required

Capillary to connect the pump to the autosampler

Follow these steps

1. On the connecting capillary, remove the knurls.

2. Guide the connecting capillary through the guide holes in the
housings of both, the pump and autosampler.

5 • Installation

Figure 26: Guide holes in the autosampler and pump (left: view from
autosampler; right: view from pump)

No. Description

1 Capillary connecting the pump and autosampler:

1a Capillary end connected to the autosampler injection valve

1b Capillary end to be connected to pump outlet

2 Guide holes in autosampler housing and pump housing:

2a Guide hole in autosampler housing (view from above)

2b Guide hole in pump housing (view from below)

3. Reattach the knurls to the connection capillary.

4. In the pump, connect the capillary to the pump outlet.

5. In the autosampler, connect the capillary to the injection valve.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 77

5 • Installation

5.7 Turning On the Pump

TIP Before turning on the power to a Vanquish system module for the

first time, verify that the chromatography software is installed on the
data system computer. When the power is turned on, the required USB
drivers are automatically found and the Windows™ operating system
can detect the device.

To turn on the power to the pump, follow these steps:

1. Check that the power button on the front left of the Vanquish
system base (system power button) is pressed in. If the power
button stands out, press the power button to turn on the power on
the system base.

2. Turn on the pump with its main power switch.

Turn the pump off with the main power switch, when instructed to do
so, for example, during maintenance. Pressing the system power button
will not be sufficient to turn off the power to the device completely.

Whenever the power to the pump is turned on, the following sequence
of events occurs:

• The pump performs a self-test.

If the self-test is not successful, the status indicators are red and the
pump is not ready for analysis. Check the Chromeleon Audit Trail for
the related message and take appropriate remedial action.

• The pump starts operation with a seal wash cycle. Before turning on

the pump, check the liquid level in the seal wash reservoir. Consider
checking also the liquid level in the waste container.

For power on/off control during pump operation, see section 6.4,

page 88.

Page 78 Vanquish Pumps (VH-P10-A-02) Operating Manual

5.8 Setting Up the Pump in the Software

This manual assumes that the chromatography software is already
installed on the data system computer and a valid license is available.

For more information about setting up the Vanquish system in the
software, refer to the Vanquish System Operating Manual.

The Help for the software that you are using provides detailed
information about the settings on each property page.

5 • Installation

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 79

5 • Installation

Page 80 Vanquish Pumps (VH-P10-A-02) Operating Manual

6 • Operation

6 Operation

This chapter describes the elements for pump control, provides

information for routine operation and for shutdown.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 81

6 • Operation

6.1 Introduction to this Chapter

The information in this chapter assumes that the initial setup of the
pump has already been completed. If this is not the case, refer to the
instructions in chapter 5 Installation before proceeding (see page 47).

For a basic description of instrument control and automated sample
analysis with the Chromeleon software, refer to the Vanquish System
Operating Manual. Details on control and operation of the pump are
available in the Chromeleon Help.

Page 82 Vanquish Pumps (VH-P10-A-02) Operating Manual

6.2 Safety Guidelines for Operation

When operating the pump, pay attention to the following safety
guidelines:

Observe all warning messages and precautionary statements presented
in section 2.3 Safety Precautions (see page 22).

CAUTION—High Luminosity of LED

The high luminosity produced by the LED illuminating the inside of the
pump can be harmful to the eyes. Do not look directly into the light
produced by the LED. Do not use light-focusing instruments for viewing
the light beam.

6 • Operation

NOTICE Pay attention also to the following guidelines:

• When operating the chromatography system, always set the lower

pressure limit for the pump. This prevents damage resulting from
leakage or from running the pump dry.

• If there is evidence of leakage in the pump, turn off the pump flow

and remedy the situation immediately.

• If the pump flow is interrupted, act appropriately to protect the

components in the detector. For details, refer to the Operating
Manual for the detector.

• Always verify that the autosampler is turned on before the pump

flow is on and pressure builds up. If the autosampler is turned off,
for example, after a power failure, stop the pump flow and wait until
the pressure is down to zero before turning on the autosampler or
other modules again.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 83

6 • Operation

6.3 Control Elements

The pump is designed to be operated mainly from a computer running
with the chromatography software.

In addition, the following elements are available on the pump:

• Keypad

The keypad buttons allow you to perform certain functions directly
from the pump.

• Status indicators

The LEDs (Light Emitting Diodes) on the status indicator LED bar on
the front side of the pump and the STATUS LED on the keypad
provide a quick visual check of the operational status of the pump.

6.3.1 Keypad

STATUS

The keypad inside the pump allows you to perform certain functions
directly from the pump. When you press a button, a short beep confirms
that the function is performed. When the pump is connected in the
Chromeleon software, some functions may not be available from the
keypad (see further down in this section).

Figure 27: Keypad

The STATUS LED provides a quick visual check of the operational status
of the pump. When the doors are closed, the LED bar on the front side
indicates the operational status.

For status details, see section 6.3.2 Status Indicators, page 86.

Page 84 Vanquish Pumps (VH-P10-A-02) Operating Manual

MUTE ALARM

SELECT

6 • Operation

If beeping alerts you that the pump firmware detected a problem, for
example leakage, beeping continues until you turn off the beep. Pressing
this button turns off the beep for the current alarm. As a standard,
beeping starts again after 10 minutes if the problem is still present or if
the firmware detected another problem.

Pressing the button selects a pump head. The LEDs next to the button
indicate which pump head is selected, with L referring to the left pump
head and R referring to the right pump head. The LED is green when the
pump head is selected. Before performing a Dock or Purge function
from the keypad, select the pump head for which you want to perform
the action.

DOCK

PURGE

Pressing the button moves the pistons of the selected pump head to the
appropriate position for maintenance procedures, such as pump head or
piston replacement, and undocks the pistons.

When undocking is complete, the LED next to the FLOW button starts
flashing green for the selected pump head. The LED continues flashing
while no pistons are docked. Pressing the button again docks the
pistons.

To avoid damage to the pump, remove or install the pump heads only
while the pistons are undocked (LED is flashing).

Pressing the button starts a purge cycle. Pressing the button while a
purge cycle is running stops the cycle.

For more information about purging, see section 6.8, Purging the Pump,

page 98.

FLOW

Pressing the button starts or stops the flow, considering the selected
flow rate, solvent composition, and flow acceleration/deceleration.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 85

6 • Operation

When the pump is connected in the Chromeleon software

The LEDs next to the button indicate the following:

LED Description

Off (dark) The pump flow is off or zero.

Green The pump head is delivering.

Green, flashing The pistons are undocked.

The button functionality is as follows when the pump is connected in the
Chromeleon software:

• No injection (sample) or sequence is running:

All functions are available from the keypad.

• An injection (sample) or sequence is running:

The Mute Alarm function remains available from the keypad,
allowing you to turn off the beep for the current alarm.

6.3.2 Status Indicators

The status LED bar on the front side of the pump and the STATUS LED on
the inside keypad provide information about the pump status.

LED Bar

The LED bar provides the information when the pump is closed. When
the pump is connected in the Chromeleon software, the LED bar may
provide less information.

In addition, the Select function remains available from the keypad,
allowing you to select a pump head.

LED Bar Description

Off (dark) The power to the pump is turned off.

Dimmed The doors of the pump are open.

Yellow, flashing slowly The power to the pump is turned on, but the pump is not

connected in the Chromeleon software.

Yellow The pump is connected in the Chromeleon software, but is

not equilibrated. The pump flow is off.

Green, flashing A purge cycle is running.

Page 86 Vanquish Pumps (VH-P10-A-02) Operating Manual

STATUS LED

6 • Operation

LED Bar Description

Green The pump is equilibrated, but no data acquisition is running.

The pump flow is on.

Blue A sample or sequence is running, including data acquisition.

Red A problem or error has occurred. For the related message,

check the Chromeleon Audit Trail. For remedial action, see
the Troubleshooting section in this operating manual.

The STATUS LED on the keypad inside the pump provides the following
information:

STATUS LED Description

Off (dark) The power to the pump is turned off.

Green The pump is functioning properly.

Red A problem or error has occurred. For the related message,

check the Chromeleon Audit Trail. For remedial action, see
the Troubleshooting section.

For information about the LEDs that are present next to a button on the
keypad, see section 6.3.1 Keypad, page 84.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 87

6 • Operation

6.4 Power On/Off Control

The power switch on the pump is the main switch for power on/off
control. The main power switch is turned on during initial installation of
the pump.

For easier handling, you can use the power button on the front left of
the Vanquish system base (system power button) for power on/off.

Observe the following:

• All modules in the Vanquish system that are connected to the

system base via system interlink cables are turned on or off
simultaneously when the system power button is pressed.

• When the power is on, the system power button is pressed in. When

the power is off, the system power button stands out.

• If the main power switch on a device is off, you cannot turn on the

device with the system power button.

• To turn off a device completely, you have to turn it off with the main

power switch on the device. Pressing the system power button will
not be sufficient to turn off the power to the device completely.

Whenever the power to the pump is turned on, the following sequence
of events occurs:

• The pump performs a self-test.

If the self-test is not successful, the status indicators are red and the
pump is not ready for analysis. Check the Chromeleon Audit Trail for
the related message and take appropriate remedial action.

• The pump starts operation with a seal wash cycle. Before turning on

the pump, check the liquid level in the seal wash reservoir. Consider
checking also the liquid level in the waste container.

Page 88 Vanquish Pumps (VH-P10-A-02) Operating Manual

6.5 Use of Solvents and Additives

Particles entering the chromatography system can block capillaries and
valves, increase wear, and damage the column or system. Especially with
aqueous solvents, algae and other microorganisms can grow and deposit
in the chromatography system and block the solvent line filters. Blocked
capillaries or filters can cause increased or unstable system pressure.

For optimum performance of the chromatography system, observe the
following guidelines:

• Be sure that the substances you are using are compatible with all

parts in the flow path.

• Use high-quality high-purity solvents (filtered) and additives as

required by the application, for example, UHPLC-grade or LC/MS­grade. If a fluorescence detector is used in the system, consider
using fluorescence-grade solvents.
Filtered high-purity solvents are usually labeled accordingly by the
vendor.

6 • Operation

• When preparing salt solutions or buffers and preparation is

complete, use membrane filtration (0.2 µm) to remove any
particulate matter and to reduce microbial growth.

• When using water, use high-quality water for example, UHPLC-grade

or LC/MS-grade (0.2 µm filtered). When you use water from water
purification systems, keep in mind that polymeric contamination
may occur if the purification system is not maintained properly.

• Before filling a solvent reservoir, always rinse the reservoir

thoroughly with a high-purity solvent.

• Use fresh solvents at regular intervals. Avoid adding up (refreshing)

solvents. With premixed solvents, be especially sure that they are
properly prepared and fresh.

• Before changing from a buffer or salt solution to organic solution,

flush the pump thoroughly with de-ionized water.

• When changing to a different type of solvent, make sure that the

new solvent is miscible with the previous solvent. If the solvents are
not miscible, flocculation may occur.
Mix immiscible solvents with an intermediate solvent to replace
them step by step. For example, use isopropanol.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 89

6 • Operation

• Always use the appropriate solvent line filters as recommended by

Thermo Fisher Scientific. Check the filter frits for permeability at
regular intervals and replace them as necessary.

• The pump is shipped with UHMW-PE piston seals. Using

tetrahydrofuran, ketones, or ammonium hydroxide as solvents can
cause swelling and damage the seals.

• For best seal performance, do not recycle or recirculate solvents.

Avoid using methanol from aluminum reservoirs.

• To reduce the growth of algae, consider using amber glassware or

using appropriate additives, for example, formic acid.

• After operation, flush out buffers and solutions that form peroxides.

• Never leave buffers, salt solutions, or aggressive solvents in the

system for a longer period without flow.

• Note the special properties of the solvents, such as viscosity, boiling

point, or UV absorption.

Page 90 Vanquish Pumps (VH-P10-A-02) Operating Manual

6.6 Preparing the Pump for Operation

This section gives information on any additional steps that are required
to prepare the pump for operation and sample analysis.

Before Operating the Pump for the First Time

Prepare the pump for the first-time operation, observing the following:

NOTICE Flush the system flow path thoroughly before operating the
pump for the first time:

• When you install devices or components to the system, always flush

them to waste before connecting them in the system flow path. To
flush the Vanquish modules, follow the instructions in the Vanquish

System Operating Manual.

6 • Operation

• Some components of the pump are filled with isopropanol when the

pump is shipped from the manufacturing site. When operating the
pump for the first time, use solvents that are miscible with
isopropanol. If they are not, use an appropriate intermediate
solvent.

• To remove the isopropanol from the pump components:

♦ Verify that the flow cell and the column are not connected in the

flow path.

♦ Purge the pump and then have the pump deliver flow for a short

time.

• Verify that any air bubbles are completely flushed out of the system

flow path.

Before Starting Sample Analysis

Before starting an analysis:

• Check the liquid level in the solvent reservoirs. Verify that the

amount of solvent is sufficient for the analysis.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 91

6 • Operation

• Verify that the doors of the modules in the Vanquish system are

closed.

• Make sure that the chromatography system is properly equilibrated.

System equilibration should include the following operations:

♦ Purging the pump (all channels, including those not used for the

application)

♦ Flushing the entire chromatography system with the starting

solvent to rinse out any solvent from a previous analysis run

♦ Warming up (or cooling down) all temperature-controlled

devices in the system to the starting temperature
Temperature-controlled devices can be, for example,

♦ Column compartment and post-column cooler

♦ Sample compartment thermostatting in the autosampler

♦ Flow cell in a fluorescence detector

♦ Evaporation tube in a charged aerosol detector

♦ Turning on the lamp (or lamps) in the UV/VIS detector

♦ Monitoring the pump pressure and pressure ripple and checking

that the pressure is stable and the ripple within reasonable
limits for the application

♦ Monitoring the detector signal and checking whether the

detector signal is stable so that the drift and signal noise are
within reasonable limits for the application

♦ Performing an autozero of the detector baseline

TIP The Chromeleon software supports procedures for automatically
starting a chromatography system in the software (Smart Startup). The
startup procedure includes the operations for system equilibration. For
details, refer to the Chromeleon Help.

Page 92 Vanquish Pumps (VH-P10-A-02) Operating Manual

6.7 Important Operating Parameters

The parameters described in this section should be considered for
routine operation of the pump. You can usually access them from the
Chromeleon user interface. If a parameter listed below is not available in
Chromeleon, consider updating the firmware and Chromeleon version.
For more information, refer to Chromeleon Help and documents.

Parameter Description

Compression The compression values of the pump head can provide

valuable information for troubleshooting. For details,
see section 8.5.2, page 211.

Curve You can specify linear or non-linear (curved) gradient

profiles.

• Curve 5 (default setting) is linear. Changes in the

composition of the delivered solvent over time are
constant.

• Curves 1 through 4 are convex upward. Convex

curves cause rapid changes in solvent composition at
the beginning of the gradient and slower changes at
the end. Slope changes over time become extreme
as curves go from 4 (least convex) to 1 (most
convex).

• Curves 6 through 9 are concave upward. Concave

curves cause slower changes in solvent composition
at the beginning of the gradient and rapid changes at
the end. Slope changes over time become extreme
as curves go from 6 (least concave) to 9 (most
concave).

In addition, you can define step gradients directly in
the gradient table (for example, step A and step B).
Thus, more than 11 different options are available to
change the gradient.

6 • Operation

Degasser The degasser is enabled as a standard when the pump

is shipped (Degasser = On). This setting is the
preferred setting.

Flow The allowed flow range is indicated in the dialog box

for the pump in the Chromeleon 7 Instrument
Configuration Manager (Chromeleon 6.8 Server
Configuration). You can change the upper and lower
limit for the flow rate within the allowed range.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 93

6 • Operation

Parameter Description

Flow ramps Set the flow acceleration and flow deceleration.

Recommendation:

Set the parameters to a value between 1/3 and factor
3 of the (column) flow rate.

Flow acceleration The flow acceleration (Maximum Flow Ramp Up)

determines how fast the pump starts delivering with
the selected flow rate. If the value is too low, it will
take correspondingly long for the pump to build up the
necessary pressure and start delivery the required
flow. If the value is too high, this may impair the
column lifetime.

Flow deceleration The flow deceleration (Maximum Flow Ramp Down)

determines how fast the pump reduces the flow. If the
value is too low, it will take correspondingly long for
the pump to reduce the flow and thus the pressure. If
the value is too high, this may impair the column
lifetime.

Leak detection Leak detection is enabled as a standard when the

pump is shipped (Leak Sensor Mode = Enabled). This
is the preferred setting.

Piston calibration value The Piston Calibration Value property must match the

3-digit calibration value that is imprinted on the rear
of the piston. You have to update the value in the
Chromeleon software, for example, when you install
new pistons (see page 144).

Piston seal washing/rear
seal washing

The seal wash system is enabled and cannot be
disabled. As a standard, the pump performs a seal
wash in the following situations:

• Once per hour

• Each time the pump is turned on

If required, you can start an additional seal wash cycle
or stop a running cycle:

• If the seal wash pump is in Idle mode, select Active

to start a wash cycle.

• If the seal wash pump is in Active mode, select Idle

to stop a running wash cycle.

You can disable the drop counter functionality by
setting Rear Seal Wash Monitoring to Off. Note that
disabling drop detection will not disable the seal wash
system. However, messages relating to the seal wash
system (for example, when the system has run out of
wash liquid) or piston seal leakage will no longer alert
you in the user interface.

Page 94 Vanquish Pumps (VH-P10-A-02) Operating Manual

6 • Operation

Parameter Description

Pressure limits The allowed pressure range is indicated in the dialog

box for the pump in the Chromeleon 7 Instrument
Configuration Manager (Chromeleon 6.8 Server
Configuration). You can adapt the upper and lower
pressure limits within the allowed range.

• The lower pressure limit helps to prevent the pump

(and column) from running dry. A typical setting is
1 MPa.

• The upper pressure limit helps to protect the column

from too high pressure. The column and the
application determine the appropriate setting.

When the pump pressure is outside the specified
limits, the Chromeleon software stops the pump flow
and aborts a running queue (or batch).

Pump pressure In the dialog box for the pump in the Chromeleon 7

Instrument Configuration Manager (Chromeleon 6.8
Server Configuration), the Pump_Pressure check box
is selected as a standard when the pump is set up in
Chromeleon. The Chromeleon software uses this
setting to generate the channel for recording the
pump pressure (which corresponds to the column
pressure).

Always record the pump pressure. If a problem occurs,
the information from the pump pressure channel can
provide helpful information to identify and eliminate
the source for the problem.

Purge Purge the pump in the following situations:

• To remove air bubbles that might be trapped in the

system

• When changing to a different type of solvent

For details, see section 6.8 Purging the Pump, page 98.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 95

6 • Operation

Parameter Description

Solvent composition In the dialog box for the pump in the Chromeleon 7

Instrument Configuration Manager (Chromeleon 6.8
Server Configuration), set the number of the solvents
to be used with the pump. You can change the
standard solvent names (%A1, %A2, %A3, %B1, %B2,
%B3) as required. The solvent names appear in the
Chromeleon user interface.

In the Chromeleon Client, set the solvent composition.
For each of the two channels, select the solvent and
percentage of solvent that you want to deliver.

• For channel A, select the solvent by setting

%A_Selector to %A1 or %A2 or %A3. For channel B,
set %B_Selector to %B1 or %B2 or %B3.

• Determine the percentage of solvent that you want

to deliver by setting the percentage for solvent B
(%B). Solvent A automatically delivers the remaining
volume.

Examples:

• To deliver 100% of solvent B2, select %B2 and set %B

to 100%.

• To deliver 100% of solvent A3, select %A3 and set %B

to 0%.

• To deliver 30% of solvent A1 and 70% of solvent B3,

select %A1 and %B3 and set %B to 70%.

Solvent consumption You can monitor the solvent consumption when you

have entered the following information for the related
solvent:

• Volume of solvent in the reservoir (at the beginning

of a sequence)

• Lower limit for the solvent in the reservoir, which is

the minimum volume of liquid that must be available
in the reservoir
The Remain Time property for the solvent reports
the time left until the liquid level is expected to reach
the lower limit. The Chromeleon software calculates
the time from the current flow rate and volume
entered.

• Warning limit when you want to be informed about

the liquid level in the reservoir

When the liquid in the reservoir reaches the lower
limit, the Chromeleon software stops the pump as
specified in the Emergency Method (or Program) if
available or aborts the queue (or batch) and stops the
pump flow.

Page 96 Vanquish Pumps (VH-P10-A-02) Operating Manual

6 • Operation

Parameter Description

Waste level You can monitor the liquid level in the waste container

when you have entered the following information:

• Volume of liquid in the waste container at the

beginning of a sequence

• Upper limit for the liquid level in the waste container

The Remain Time property for the waste reports the
time left until the liquid level is expected to reach the
upper limit. The Chromeleon software calculates the
time from the current flow rate and calculated
current liquid level.

• Warning limit when you want to be informed about

the liquid level in the waste container

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 97

6 • Operation

6.8 Purging the Pump

Purging the pump means rinsing the pump for a short time at a higher
flow rate, using the selected solvents. From the purge unit, the solvent is
automatically dispensed to waste.

When

• The pump is operated for the first time

To flush out the isopropanol that is present in some components of
the pump when the pump is shipped from the manufacturing site

• One or more solvent lines have been empty

• To remove air bubbles that might be trapped in the system (pump

head, solvent lines) because air bubbles in the system may lead to
the following problems:

Items required

Settings

♦ Pressure pulsation

♦ High noise level or pulsation during pump operation

♦ Non-reproducible analysis

• When changing to a different type of solvent, for example, when

changing to a different analytical method

Solvents suitable for purging

To flush out the isopropanol when operating the pump for the first time,
use solvents that are miscible with isopropanol. If they are not, use an
appropriate intermediate solvent.

The following settings are considered for the purge cycle:

Setting Description

Purge flow The standard setting is 5 mL/min.

You can adapt the setting in Chromeleon if required. The setting
is considered also when purging the pump from the keypad.

Purge time The standard purge time is 5 minutes.

You can adapt the setting in Chromeleon if required. The setting
is considered also when purging the pump from the keypad.

Page 98 Vanquish Pumps (VH-P10-A-02) Operating Manual

Setting Description

6 • Operation

Channel to be
purged and
solvent to be used

To Purge the Pump

Select one of the following alternatives:

• Purge the pump from Chromeleon.

• Purge the pump from the keypad.

Purging the Pump from Chromeleon

To purge the pump from Chromeleon, follow these steps:

1. For each channel, select the solvent that you want to use for
purging. The solvent names below refer to the standard solvent
names.
For channel A, select the solvent by setting %A_Selector to %A1 or
%A2 or %A3. For channel B, set %B_Selector to %B1 or %B2 or %B3.

2. Determine the percentage of solvent that you want to deliver by
setting the percentage for solvent B (%B). Solvent A automatically
delivers the remaining volume.

Select the channel that you want to purge and the solvent to be
used. For details, see further down in this section.

Recommended: The channel to be purged should deliver 100% of
the volume.

Examples:

♦ To purge channel B with solvent B1 delivering 100%, select %B1

and set %B to 100%.

♦ To purge channel A with solvent A3 delivering 100%, select %A3

and set %B to 0%.

3. Optional
Adapt the settings for the purge flow or purge time if required.

4. Start the purge cycle.
The purge cycle ends automatically when the specified purge time
ends. If you want to stop purging before the purge time ends, turn
off the purge from Chromeleon.

5. Perform a purge cycle for all channels that you want to purge.

Vanquish Pumps (VH-P10-A-02) Operating Manual Page 99

6 • Operation

Purging the Pump from the Keypad

To purge the pump from the keypad, follow these steps:

1. Select the channels that you want to purge.
On the keypad, check the LEDs next to the Select button. A green
LED (L or R or both) indicates that the pump head (channel) is
selected. Note the following:

♦ The purge cycle will be performed for the selected pump head

with the most recently used solvent.

♦ When both pump heads are selected, each channel is purged

with the most recently used solvent delivering 50 % of the
volume.

♦ When the pump is operated for the first time, the purge cycle

will be performed for solvent 1 of the selected pump head (A1
or B1, respectively).

Recommended: The channel to be purged should deliver 100% of
the volume. Check and adapt the setting in Chromeleon if
considered necessary.

2. Optional (in Chromeleon)
Adapt the settings for the purge flow or purge time if required.

3. To start the purge cycle, press the Purge button.
Note the following:

♦ The purge cycle ends automatically when the specified purge

time ends.

♦ Pressing the button while a purge cycle is running stops the

cycle.

Page 100 Vanquish Pumps (VH-P10-A-02) Operating Manual