Thermo Scientific Vanquish VH-P10-A-02 Operating Manual
thermoscientific
Vanquish
Pumps
VH-P10-A-02
Operating Manual
4820.4411-EN Revision 1.0 • October 2017
Copyright © 2017 Thermo Fisher Scientific Inc. All rights reserved.
Original Operating Manual
The hardware descriptions in this manual revision refer to pumps VH-P10-A-02. For the manual
release history, see page 229.
Trademarks
MP35N is a trademark of SPS Technologies. PharMed is a trademark of Saint-Gobain
Performance Plastics. Torx is a trademark of TEXTRON INDUSTRIES, Incorporated. Acrobat,
Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated. Microsoft, Windows,
and Windows Vista are trademarks of Microsoft Corporation.
All other trademarks are property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to
use in the product operation. The document is copyright protected; any reproduction of the
whole or any part of this document is strictly prohibited, except with the written authorization
of Thermo Fisher Scientific Inc.
This manual is provided "as is." The contents of this manual are subject to being changed,
without notice, in future revisions.
Thermo Fisher Scientific Inc. makes no representations that this document is complete,
accurate, or error-free. Thermo Fisher Scientific Inc. assumes no responsibility and will not be
liable for any errors, omissions, damage, or loss that might result from any use of this document,
even if the information in the document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any Terms and Conditions of Sale.
The Terms and Conditions of Sale shall govern all conflicting information between the two
documents.
Printed manual version only
Printed in Germany on 100% chlorine-free bleached, high-white paper that is produced in an
environmentally friendly process, leading to a paper profile of zero CO
emissions.
2
Manufacturer's address
Dionex Softron GmbH, Part of Thermo Fisher Scientific
Dornierstrasse 4, D-82110 Germering
Contacting Us
Ordering Information
Technical Assistance
Contacting Us
There are several ways to contact us:
For ordering information or sales support for HPLC products, contact
your local Thermo Fisher Scientific sales organization. For contact
information, go to Contact Us on http://www.thermofisher.com.
For technical support for HPLC products, contact your local Thermo
Fisher Scientific support organization. For contact information, go to
Contact Us on http://www.thermofisher.com.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 3
Contacting Us
Page 4 Vanquish Pumps (VH-P10-A-02) Operating Manual
Contents
1 Using this Manual ...........................................................................11
2 Safety ..............................................................................................17
Contents
1.1 About this Manual ................................................................................................ 12
1.2 Conventions .......................................................................................................... 13
1.2.1 Safety Messages ....................................................................................... 13
1.2.2 Special Notices and Informational Notes ................................................. 13
1.2.3 Typographical Conventions ...................................................................... 14
1.3 Reference Documentation .................................................................................... 15
2.1 Safety Symbols and Signal Words ......................................................................... 18
2.1.1 Safety Symbols and Signal Words in This Manual ................................... 18
2.1.2 Observing this Manual ............................................................................. 19
2.1.3 Safety Symbols on the Pump ................................................................... 19
2.1.4 Rating Plate .............................................................................................. 20
2.2 Intended Use ......................................................................................................... 21
2.3 Safety Precautions ................................................................................................ 22
2.3.1 General Safety Information ..................................................................... 22
2.3.2 Qualification of the Personnel ................................................................. 23
2.3.3 Personal Protective Equipment ............................................................... 23
2.3.4 Electrical Safety Precautions .................................................................... 24
2.3.5 General Residual Hazards ........................................................................ 25
2.3.6 In Case of Emergency ............................................................................... 26
2.4 Solvent and Additive Information ......................................................................... 27
2.5 Compliance Information ....................................................................................... 29
3 Pump Overview ...............................................................................31
3.1 Pump Features ...................................................................................................... 32
3.2 Operating Principle ............................................................................................... 33
3.3 Interior Components............................................................................................. 35
3.4 Piston Seal Wash System ...................................................................................... 37
3.5 Purge Unit ............................................................................................................. 38
3.6 Leak Detection ...................................................................................................... 39
3.7 Operation .............................................................................................................. 40
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 5
Contents
4 Unpacking ....................................................................................... 41
4.1 Unpacking ............................................................................................................ 42
4.2 Scope of Delivery ................................................................................................. 45
5 Installation ...................................................................................... 47
5.1 Safety Guidelines for Installation ......................................................................... 48
5.2 Installing the Pump .............................................................................................. 49
5.3 Site Requirements ................................................................................................ 51
5.4 Accessing the Interior Components ..................................................................... 53
5.5 Setting Up the Hardware ..................................................................................... 54
5.5.1 System Arrangement............................................................................... 54
5.5.2 Connecting the Pump .............................................................................. 56
5.6 Setting Up the Flow Connections ......................................................................... 59
5.6.1 General Information and Guidelines ....................................................... 59
5.6.2 Guiding Capillaries and Tubing Through the System .............................. 60
5.6.3 Connecting Fittings, Capillaries, and Tubing ........................................... 62
5.6.4 Guiding Liquids to Waste ........................................................................ 64
5.6.5 Connecting the Solvent Lines .................................................................. 65
5.6.6 Setting Up the Seal Wash System ........................................................... 69
5.6.7 Connecting the Pump and Autosampler ................................................. 77
5.7 Turning On the Pump ........................................................................................... 78
5.8 Setting Up the Pump in the Software .................................................................. 79
6 Operation ....................................................................................... 81
6.1 Introduction to this Chapter ................................................................................ 82
6.2 Safety Guidelines for Operation .......................................................................... 83
6.3 Control Elements.................................................................................................. 84
6.3.1 Keypad ..................................................................................................... 84
6.3.2 Status Indicators ...................................................................................... 86
6.4 Power On/Off Control .......................................................................................... 88
6.5 Use of Solvents and Additives .............................................................................. 89
6.6 Preparing the Pump for Operation ...................................................................... 91
6.7 Important Operating Parameters ........................................................................ 93
6.8 Purging the Pump................................................................................................. 98
Page 6 Vanquish Pumps (VH-P10-A-02) Operating Manual
Contents
6.9 Optimizing the Performance of the Pump .......................................................... 101
6.9.1 General Guidelines ................................................................................. 101
6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple .............................. 102
6.9.3 Installing Solvent Shut Off Valves .......................................................... 109
6.10 Shutting Down the Pump .................................................................................... 111
6.10.1 Short-Term Shutdown (Interruption of Operation) ............................... 111
6.10.2 Long-Term Shutdown ............................................................................. 112
7 Maintenance and Service .............................................................. 115
7.1 Introduction to Maintenance and Service .......................................................... 116
7.2 Safety Guidelines for Maintenance and Service ................................................. 117
7.3 General Rules for Maintenance and Service ...................................................... 119
7.4 Routine and Preventive Maintenance ................................................................ 120
7.4.1 Maintenance Plan .................................................................................. 120
7.4.2 Flushing the Pump ................................................................................. 122
7.4.3 Cleaning or Decontaminating the Pump ................................................ 124
7.4.4 Predictive Performance ......................................................................... 126
7.5 Seal Wash System ............................................................................................... 128
7.5.1 Testing the Seal Wash System for Leakage ............................................ 128
7.5.2 Replacing the Seal Wash Lines ............................................................... 129
7.5.3 Replacing the Seal Wash Detector ......................................................... 132
7.6 Y-Connector ........................................................................................................ 134
7.7 Pump Head.......................................................................................................... 136
7.7.1 Overview of Pump Head Parts ............................................................... 137
7.7.2 Replacing the Pump Head ...................................................................... 138
7.7.3 Replacing the Pistons ............................................................................. 142
7.7.4 Cleaning the Piston ................................................................................ 145
7.7.5 Replacing the Piston Seals or Support Ring ........................................... 146
7.7.6 Recommendations for New Piston Seals ............................................... 151
7.7.7 Replacing the Seal Wash Seals ............................................................... 153
7.7.8 Replacing the Pump Head Seals ............................................................. 158
7.7.9 Testing the Piston Seals for Leakage ...................................................... 161
7.8 Check Valves ....................................................................................................... 163
7.8.1 Replacing the Inlet Check Valves ........................................................... 163
7.8.2 Replacing the Outlet Check Valves ........................................................ 165
7.8.3 Cleaning the Check Valves ..................................................................... 166
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 7
Contents
7.9 Inline Filter ......................................................................................................... 167
7.9.1 Testing the Inline Filter for Permeability .............................................. 167
7.9.2 Replacing the Inline Filter or Capillary Mixer ........................................ 168
7.10 Mixing System .................................................................................................... 170
7.10.1 Testing the Static Mixer for Permeability ............................................. 170
7.10.2 Replacing the Static Mixer or Capillary Mixer ....................................... 171
7.11 Solvent Lines and Solvent Line Filters ................................................................ 173
7.11.1 Emptying the Solvent Lines ................................................................... 173
7.11.2 Replacing Solvent Lines ......................................................................... 173
7.11.3 Replacing the Solvent Line Filter ........................................................... 176
7.12 Testing the Pump for Leakage ........................................................................... 178
7.13 Replacing the Main Power Fuses ....................................................................... 180
7.14 Updating the Pump Firmware ............................................................................ 182
7.15 Replacing the Doors ........................................................................................... 184
7.16 Transporting or Shipping the Pump ................................................................... 186
7.16.1 Preparing the Pump for Transport ........................................................ 187
7.16.2 Transporting the Pump to a New Location ........................................... 188
7.16.3 Shipping the Pump ................................................................................ 188
7.17 Replacing the Slide-In Module ........................................................................... 190
7.17.1 Removing the Slide-In Module .............................................................. 190
7.17.2 Installing the Slide-In Module ............................................................... 192
8 Troubleshooting ............................................................................ 195
8.1 General Information about Troubleshooting .................................................... 196
8.2 Messages ............................................................................................................ 199
8.3 Testing From Chromeleon ................................................................................. 207
8.4 Resolving Leakage .............................................................................................. 208
8.5 Pressure Problems or Shifts in Retention Time ................................................. 210
8.5.1 Introduction .......................................................................................... 210
8.5.2 Resolving Pressure Pulsation or Shifts In Retention Time .................... 211
8.5.3 Performing Pressure Recalibration ....................................................... 213
8.5.4 Testing the Inline Filter/Static Mixer for Permeability .......................... 214
9 Specifications ................................................................................ 215
9.1 Performance Specifications ............................................................................... 216
9.2 Physical Specifications ....................................................................................... 218
Page 8 Vanquish Pumps (VH-P10-A-02) Operating Manual
Contents
10 Accessories, Consumables and Replacement Parts ...................... 219
10.1 General Information ........................................................................................... 220
10.2 Ship Kit ................................................................................................................ 221
10.3 Optional Accessories ........................................................................................... 222
10.4 Consumables and Replacement Parts ................................................................ 223
11 Appendix ..................................................................................... 227
11.1 Compliance Information ..................................................................................... 228
11.1.1 Declarations of Conformity .................................................................... 228
11.1.2 WEEE Compliance .................................................................................. 229
11.1.3 FCC Compliance ..................................................................................... 229
11.1.4 Manual Release History ......................................................................... 229
11.2 Digital I/O ............................................................................................................ 230
12 Index ........................................................................................... 233
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 9
Contents
Page 10 Vanquish Pumps (VH-P10-A-02) Operating Manual
1 • Using this Manual
1 Using this Manual
This chapter provides information about this manual, the conventions used
throughout the manual, and the reference documentation that is available
in addition to this manual.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 11
1 • Using this Manual
1.1 About this Manual
This manual describes the functional features and operating principle of
your Vanquish™ pump and provides instructions for installation, set up,
start up, shut down, operation, maintenance and troubleshooting.
The layout of this manual is designed to provide quick reference to the
sections of interest to the user. To obtain a full understanding of your
pump, read this manual thoroughly.
This manual also contains safety messages, precautionary statements,
and special notices that can prevent personal injury, damage to the
pump, or loss of data when followed properly.
Note the following:
• The pump configuration may vary; therefore, not all descriptions
necessarily apply to your particular pump.
• If some detail applies to only one model or variant, the model or
variant is identified by name.
• Illustrations in this manual are provided for basic understanding.
They can vary from the actual model of the pump or component.
However, this does not influence the descriptions. No claims can be
derived from the illustrations in this manual.
The descriptions in this manual assume that the pump is installed in the
Vanquish system stack. If this is not the case, additional hardware is
required and must be ordered separately. The information in this
manual applies correspondingly.
Page 12 Vanquish Pumps (VH-P10-A-02) Operating Manual
1.2 Conventions
This section describes the conventions that are used throughout this
manual.
1.2.1 Safety Messages
The safety messages and precautionary statements in this manual
appear as follows:
• Safety messages or precautionary statements that apply to the
• Safety messages or precautionary statements that apply to an entire
1 • Using this Manual
entire manual and all procedures in this manual are grouped in the
Safety chapter.
section or to multiple procedures in a section appear at the
beginning of the section to which they apply.
• Safety messages that apply to only a particular section or procedure
appear in the section or procedure to which they apply. They appear
different from the main flow of text.
Safety messages are often preceded by an alert symbol and/or alert
word. The alert word appears in uppercase letters and in bold type.
Make sure that you understand and follow all safety messages
presented in this manual.
1.2.2 Special Notices and Informational Notes
Special notices and informational notes in this manual appear different
from the main flow of text. They appear in boxes and a note label
identifies them. The label text appears in uppercase letters and in bold
type.
NOTICE Highlights information necessary to prevent damage to the
pump or invalid test results.
TIP Highlights information of general interest or helpful information that
can make a task easier or optimize the performance of the pump.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 13
1 • Using this Manual
1.2.3 Typographical Conventions
These typographical conventions apply to the descriptions in this
manual:
Data Input and Output
• The following appears in bold type:
♦ Input that you enter by the keyboard or that you select with the
mouse
♦ Buttons that you click on the screen
♦ Commands that you enter by the keyboard
♦ Names of, for example, dialog boxes, properties, and parameters
• For brevity, long expressions and paths appear in the condensed
form, for example: Click Start > All Programs > Thermo
Chromeleon 7 > Services Manager > Start Instrument Controller.
References and Messages
• References to additional documentation appear italicized.
• Messages that appear on the screen are identified by quotation
marks.
Viewpoint
If not otherwise stated, the expressions left and right in this manual
always refer to the viewpoint of a person that is facing the pump from
the front.
Particularly Important Words
Particularly important words in the main flow of text appear italicized.
Electronic Manual Version (PDF)
The electronic version (PDF) of the manual contains numerous links that
you can click to go to other locations within the manual. These include:
• Table of contents entries
• Index entries
• Cross-references (in blue text), for example, to sections and figures
Page 14 Vanquish Pumps (VH-P10-A-02) Operating Manual
1.3 Reference Documentation
In addition to this operating manual, other documentation is available
for reference.
Hardware Documentation
Additional hardware documentation includes the following:
• Operating manuals for the other modules of the Vanquish system
A printed version of the manual is shipped with the device.
• Vanquish System Operating Manual
A printed version of the manual is shipped with the Vanquish system
base and solvent rack.
• Instrument Installation Qualification Operating Instructions
1 • Using this Manual
TIP Electronic versions of these manuals are available as PDF (Portable
Document Format) files. To open and read the PDF files, Adobe™
Reader™ or Adobe™ Acrobat™ is required.
Software Documentation
Additional software documentation includes the following:
• Chromeleon™ 7 Help and documents
The Chromeleon 7 Help provides extensive information and
comprehensive reference material for all aspects of the software.
For basic information about device installation and configuration,
refer to the Installation Guide; for specific information about a
certain device, refer to the Instrument Configuration Manager Help.
In Chromeleon 7, devices are called modules.
For information about the main elements of the user interface and
step-by-step guidance through the most important workflows, refer
to the Quick Start Guide.
For a concise overview of the most important workflows, refer to
the Reference Card.
• Chromeleon™ 6.8 Help
The Chromeleon 6.8 Help provides extensive information for all
aspects of the software, including device installation and
configuration.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 15
1 • Using this Manual
Third-Party Documentation
TIP The Chromeleon Help and documents are included in the software
shipment.
Refer also to the user documentation provided by the manufacturers of
third-party components and materials, for example, Safety Data Sheets
(SDSs).
Page 16 Vanquish Pumps (VH-P10-A-02) Operating Manual
2 • Safety
2 Safety
This chapter provides general and specific safety information and informs
about the intended use of the pump.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 17
2 • Safety
2.1 Safety Symbols and Signal Words
2.1.1 Safety Symbols and Signal Words in This Manual
This manual contains safety messages to prevent injury of the persons
using the pump. The safety symbols and signal words in this manual
include the following:
Always be aware of the safety information. Do not proceed until you
have fully understood the information and consider the consequences of
what you are doing.
CAUTION Indicates a hazardous situation that, if not avoided, could
result in minor or moderate injury.
WARNING Indicates a hazardous situation that, if not avoided, could
result in serious injury.
Page 18 Vanquish Pumps (VH-P10-A-02) Operating Manual
2.1.2 Observing this Manual
Observe the following:
• Before installing or operating the pump, read this manual carefully
to be familiar with the pump and this manual. The manual contains
important information with regard to user safety as well as use and
care of the pump.
• Always keep the manual near the pump for quick reference.
• Save this manual and pass it on to any subsequent user.
Read, understand, and comply with all safety messages and
precautionary statements presented in this manual.
2 • Safety
2.1.3 Safety Symbols on the Pump
The table lists the safety symbols that appear on the pump or on labels
affixed to the pump. Follow the safety notices in this manual to prevent
the risk of operator injury or damage to the pump.
Symbol Description
Indicates a potential hazard. Refer to this manual to avoid the risk of
personal injury and/or to prevent damage to the device.
—
Ο
Power supply is on
Power supply is off
Indicates alternating current.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 19
2 • Safety
2.1.4 Rating Plate
The rating plate is present on the pump near the electrical connections.
The rating plate indicates the serial number, part number, module
name, revision number (if any), line and fuse rating, and the
manufacturer's address.
TIP An additional type label on the leak tray of the pump indicates the
module name, serial number, part number, and revision number (if any).
To facilitate device identification have the information from this label
available when communicating with Thermo Fisher Scientific.
Page 20 Vanquish Pumps (VH-P10-A-02) Operating Manual
2.2 Intended Use
The pump is intended to be part of the Vanquish system.
The intended use of the Vanquish system is to analyze mixtures of
compounds in sample solutions. The pump is for use by qualified
personnel and in laboratory environment only.
The pump and Vanquish system are intended for laboratory research
use only. They are not intended for use in diagnostic procedures.
Laboratory Practice
Thermo Fisher Scientific recommends that the laboratory in which the
Vanquish system is used follow best practices for LC analyses. This
includes among others:
2 • Safety
• Using appropriate standards
• Regularly running calibration
• Establishing shelf life limits and following them for all consumables
used with the system
• Running the system according to the laboratory's verified and
validated 'lab developed test' protocol
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 21
2 • Safety
2.3 Safety Precautions
2.3.1 General Safety Information
All users must observe the general safety information presented in this
section and all specific safety messages and precautionary statements
elsewhere in this manual during all phases of installation, operation,
troubleshooting, maintenance, shutdown, and transport of the pump.
If the pump is used in a manner not specified by Thermo Fisher Scientific,
the protection provided by the pump could be impaired. Observe the
following:
• Operate the pump only within its technical specifications.
• Use only the replacement parts and additional components, options,
and peripherals specifically authorized and qualified for the pump by
Thermo Fisher Scientific.
Safety Standard
• Perform only the procedures that are described in this operating
manual and in supporting documents for the pump.
Follow all instructions step by step and use the tools recommended
for the procedure.
• Open the enclosure of the pump and other components only if
specifically instructed to do so in this manual.
• Thermo Fisher Scientific cannot be held liable for any damage,
material or otherwise, resulting from inappropriate or improper use
of the pump. If there is any question regarding appropriate usage,
contact Thermo Fisher Scientific before proceeding.
This device is a Safety Class I instrument (provided with terminal for
protective grounding). The device has been manufactured and tested
according to international safety standards.
Page 22 Vanquish Pumps (VH-P10-A-02) Operating Manual
2.3.2 Qualification of the Personnel
Observe the information below on the proper qualification of the
personnel installing and/or operating the pump.
Installation
Only skilled personnel are permitted to install the pump and to establish
the electrical connections according to the appropriate regulations.
Thermo Fisher Scientific recommends always having service personnel
certified by Thermo Fisher Scientific perform the installation (for brevity,
referred to as Thermo Fisher Scientific service engineer).
If a person other than a Thermo Fisher Scientific service engineer installs
and sets up the module, the installer is responsible for ensuring the
safety of the module and system.
2 • Safety
General Operation
The pump is designed to be operated only by trained and qualified
personnel in a laboratory environment.
All users must know the hazards presented by the pump and the
substances they are using. All users should observe the related Safety
Data Sheets (SDSs).
2.3.3 Personal Protective Equipment
Wear personal protective equipment and follow good laboratory
practice to protect you from hazardous substances. The appropriate
equipment depends on the hazard. For advice on the hazards and the
equipment required for the substances you are using, refer to the
material handling and safety data sheet provided by the vendor.
An eyewash facility and a sink should be available nearby. If any
substance contacts your skin or eyes, wash the affected area and seek
medical attention.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 23
2 • Safety
Protective Clothing
To protect you from chemical splashes, harmful liquids, or other
contamination, put on appropriate protective clothing, such as a lab
coat.
Protective Eyewear
To prevent liquids from striking your eyes, put on appropriate protective
eyewear, such as safety glasses with side shields. If there is a risk of
splashing liquids, put on goggles.
Gloves
To protect you from harmful liquids and avoid personal injury during
maintenance or service, put on appropriate protective gloves.
2.3.4 Electrical Safety Precautions
WARNING— Electric Shock or Damage to the Device
High voltages are present inside the device that could cause an electric
shock or damage to the device.
• Do not make any changes to the electrical or grounding connections.
• If you suspect any kind of electrical damage, disconnect the power
cord and contact Thermo Fisher Scientific Technical Support for
assistance.
• Do not open the housing or remove protective panels unless
specifically instructed to do so in this manual.
• Do not place liquid reservoirs directly upon the device. Liquid might
leak into the device and get into contact with electronic components
causing a short circuit. Instead, place liquid reservoirs in the solvent
rack that is available for the Vanquish system.
Page 24 Vanquish Pumps (VH-P10-A-02) Operating Manual
2.3.5 General Residual Hazards
Pay attention to the following general residual hazards when working
with the pump:
WARNING—Hazardous Substances
Solvents, mobile phases, samples, and reagents might contain toxic,
carcinogenic, mutagenic, infectious, or otherwise harmful substances.
The handling of these substances can pose health and safety risks.
• Be sure that you know the properties of all substances that you are
using. Avoid exposure to harmful substances. If you have any doubt
about a substance, handle the substance as if it is potentially
harmful.
• Wear personal protective equipment as required by the hazard and
follow good laboratory practice.
2 • Safety
• Reduce the volume of substances to the minimum volume required
for sample analysis.
• Do not operate the pump in a potentially flammable environment.
• Avoid accumulation of harmful substances. Make sure that the
installation site is well ventilated.
• Dispose of hazardous waste in an environmentally safe manner that
is consistent with local regulations. Follow a regulated, approved
waste disposal program.
WARNING—Biohazard
Biohazardous material, for example microorganisms, cell cultures,
tissues, body fluids, and other biological agents can transmit infectious
diseases. To avoid infections with these agents:
• Assume that all biological substances are at least potentially
infectious.
• Wear personal protective equipment as required by the hazard and
follow good laboratory practice.
• Dispose of biohazardous waste in an environmentally safe manner
that is consistent with local regulations. Follow a regulated,
approved waste disposal program.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 25
2 • Safety
WARNING—Self-Ignition of Solvents
Solvents with a self-ignition temperature below 150 °C might ignite
when in contact with a hot surface (for example, due to leakage in the
chromatography system). Avoid the use of these solvents.
WARNING—Hazardous Vapors
Mobile phases and samples might contain volatile or flammable
solvents. The handling of these substances can pose health and safety
risks.
• Avoid accumulation of these substances. Make sure that the
installation site is well ventilated.
• Avoid open flames and sparks. Do not operate the pump in the
presence of flammable gases or fumes.
CAUTION—Allergic Reaction
Some capillaries in the Vanquish system are made of MP35N™, a nickelcobalt based alloy. Individuals with sensitivity to nickel/cobalt may show
an allergic reaction from skin contact.
CAUTION—Sparking due to Electrostatic Discharge
Liquid flowing through capillaries can generate static electricity. This
effect is particularly present with insulating capillaries and nonconductive solvents (for example, pure acetonitrile). Discharge of
electrostatic energy might lead to sparking, which could constitute a fire
hazard. Prevent the generation of static electricity near the
chromatography system.
2.3.6 In Case of Emergency
WARNING—Safety Hazard
In case of emergency, disconnect the pump from the power line.
Page 26 Vanquish Pumps (VH-P10-A-02) Operating Manual
2.4 Solvent and Additive Information
To protect optimal functionality of the Vanquish system, observe these
recommendations on the use of solvents and additives:
• The system must be used with reversed-phase (RP) compatible
solvents and additives only.
• Use only solvents and additives that are compatible with all parts in
the flow path.
For details about the materials that are used in the flow path of the
pump, see the Specifications chapter. For information about the
materials that are used in the flow path of the other modules in the
Vanquish system, refer to the Specifications chapter in the
Operating Manual for the module.
Observe the general guidelines and recommendations on the use of
solvents and additives in the chromatography system (see
section 6.5, page 89).
2 • Safety
• Allowed ranges and concentrations (standard system configuration):
♦ pH range: 2-12
♦ Chloride concentration: 1 mol/L or less
• Observe the following:
♦ pH values higher than 9.5 with optical detectors
Avoid using mobile phases with a pH value higher than 9.5
together with optical detectors. This can impair the functionality
and optical performance of the detector flow cell.
♦ pH values of 2 or less or high chloride concentration
With pH values of 2 or less or high chloride concentration, the
application time should be as short as possible. Flush the system
thoroughly after these applications.
♦ Mobile phases containing ammonium hydroxide
In rare cases, a shortened lifetime of reversed-phase (UHMWPE) piston seals has been observed with high pH, ammonium
hydroxide containing mobile phases and prolonged exposure.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 27
2 • Safety
NOTICE If the system flow path includes a Vanquish charged aerosol
detector, observe the specific solvent and additive information for
this detector. Refer to the operating manual for the Vanquish
charged aerosol detector.
• Follow any specific recommendations presented in other sections of
this manual. Refer also to the operating manuals for all modules in
the Vanquish system. They may provide additional guidelines and
information.
Page 28 Vanquish Pumps (VH-P10-A-02) Operating Manual
2.5 Compliance Information
Thermo Fisher Scientific performs complete testing and evaluation of its
products to ensure full compliance with applicable domestic and
international regulations. When the device is delivered to you, it meets
all pertinent electromagnetic compatibility (EMC) and safety standards
as described in this manual. For details, see section 11.1, page 228.
Changes that you make to the device may void compliance with one or
more of these EMC and safety standards. Changes to the device include
replacing a part or adding components, options, or peripherals not
specifically authorized and qualified for the product by Thermo Fisher
Scientific. To ensure continued compliance with EMC and safety
standards, replacement parts and additional components, options, and
peripherals must be ordered from Thermo Fisher Scientific or one of its
authorized representatives.
2 • Safety
The device has been shipped from the manufacturing site in a safe
condition.
Vanquish Pumps (VH-P10-A-02) Operating Manual Page 29
2 • Safety
Page 30 Vanquish Pumps (VH-P10-A-02) Operating Manual
Loading...