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Sorvall BIOS A
Instruction Manual
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Thermo Scientific Sorvall BIOS A Instruction Manual

Thermo Scientific Sorvall BIOS A Centrifuge and Heavy Duty Centrifuge
Instruction Manual
50154616-c • 03 / 2021
Visit us online to register your warranty:
thermofisher.com/labwarranty
Table of Contents
Preface .................................................vi
Items Supplied ....................................................vi
Intended Use for Sorvall BIOS A and Sorvall BIOS A Heavy Duty ...........vii
Associated Rotors and Accessories.........................................................................................vii
Precautions ...................................................... vii
Set Up Conditions .................................................................................................................. vii
Preparation .......................................................................................................................... viii
Hazardous Substances .......................................................................................................... viii
Operating ...............................................................................................................................ix
Maintenance ..........................................................................................................................ix
Symbols used on the Centrifuge ......................................x
Symbols used in the Manual .........................................xi
Transport and Set Up .....................................I–1
1. Location ......................................................I–1
2. Transporting ...................................................I–2
3. Setting Up ....................................................I–3
Setting Up ........................................................................................................................... I–3
Level the Centrifuge ............................................................................................................. I–4
4. Mains Connection ..............................................I–5
5. Storage .......................................................I–5
6. Shipping ......................................................I–6
ThermoScientific
Centri-Touch User Interface ............................... II–1
Status .........................................................II–2
Run Parameters .................................................II–3
Control and Configuration .........................................II–3
Lighthouse Mode ................................................II–4
Operation .............................................III–1
1. Switch on the Centrifuge .......................................III–1
2. Open the Centrifuge Door .......................................III–1
ii
3. Install a Rotor ................................................III–2
3. 1. Before Installation .................................................................................................III–2
3. 2. Rotor Installation ..................................................................................................III–2
4. Maximum Loading .............................................III–5
Use of Tubes and Consumables ...........................................................................................III–6
5. Close the Centrifuge Door .......................................III–6
6. Entering Parameters ...........................................III–6
6. 1. Acceleration / Deceleration Profiles .......................................................................III–6
6. 2. Preselect Speed / RCF-Value ................................................................................III–6
6. 3. Runtime Preselection ............................................................................................III–7
6. 4. Preselect Temperature ..........................................................................................III–7
6. 5. Pre-warm or Pre-cool the Centrifuge .....................................................................III–7
7. Programs ....................................................III–8
8. Centrifugation ................................................III–9
8. 1. Start ....................................................................................................................III–9
8. 2. Stop ....................................................................................................................III–9
8. 3. Using the Drain Box ............................................................................................III–10
9. Remove a Rotor ............................................. III–11
10. Switch off the Centrifuge .................................... III–12
Maintenance and Care ...................................IV–1
1. Cleaning Intervals ............................................ IV–1
2. Basics ...................................................... IV–1
2. 1. Rotor and Accessories Inspection ......................................................................... IV–2
3. Cleaning .................................................... IV–3
3. 1. Touchscreen ....................................................................................................... IV–3
3. 2. Filter Mat ............................................................................................................ IV–4
4. Disinfection ................................................. IV–5
5. Decontamination ............................................. IV–5
6. Autoclaving ................................................. IV–6
7. Maintenance ................................................ IV–6
7. 1. Preventive Maintenance ....................................................................................... IV–6
7. 2. Service ............................................................................................................... IV–6
8. Shipping and Disposal ........................................ IV–7
Troubleshooting .........................................V-1
1. Mechanical Emergency Door Release ............................. V-1
iii
2. Ice Formation ................................................. V-2
3. Troubleshooting by Guide ....................................... V-3
4. Information for the Customer Service ............................. V-4
Technical Specifications ................................. A–1
1. Directives, Standards and Guidelines .............................A–2
2. Refrigerants ..................................................A–2
3. Mains Supply .................................................A–3
4. Accessories ..................................................A–3
Rotors ................................................ B–1
Items Supplied for Rotor ..........................................B–1
Fiberlite F6-10x1000 LEX Rotor .....................................B–2
Chemical Compatibility Chart ............................. C–1
iv
Table of Figures
Figure I–1: Centrifuge clearance, example with barcode scanner .......................................... I–2
Figure I–2: Position of the bubble in the water level ............................................................. I–4
Figure I–3: Mains connection on the backside of the centrifuge ............................................ I–5
Figure III–1: Overview centrifuge and rotor ..........................................................................III–2
Figure III–2: Rotor installation .............................................................................................III–3
Figure III–3: Tighten rotor on the drive shaft, e.g. HAEMAFlex 12 .........................................III–3
Figure III–4: Correct Loading ..............................................................................................III–4
Figure III–5: Incorrect Loading ............................................................................................III–4
Figure III–6: Put the rotor lid on the rotor body ....................................................................III–5
Figure III–7: Select program ...............................................................................................III–8
Figure III–8: Drain box (75007730)...................................................................................III–10
Figure III–9: Installing the drain box (75007730) ...............................................................III–10
Figure III–10: Remove the plug .........................................................................................III–10
Figure III–11: Remove rotor lid from rotor body .................................................................III–11
Figure III–12: Disengage the rotor locking pin from drive shaft ...........................................III–12
Figure III–13: Remove the rotor ........................................................................................III–12
Figure V–1: Marking on rotor lid ..........................................................................................V-1
Figure V–2: Position of the mechanical emergency door release ...........................................V-2
Figure V–3: Example for an error message ..........................................................................V-3
Table of Tables
Table Preface–1: Items Supplied ............................................................................................vi
Table Preface–2: Signal Words and Colors ............................................................................. vii
Table Preface–3: Symbols used on the centrifuge ....................................................................x
Table Preface–4: Symbols used in the manual ........................................................................xi
Table I–1: Overview of necessary tools for setting up ........................................................... I–3
Table V–1: Error messages .................................................................................................V-3
Table A–1: Technical Data Thermo Scientific Sorvall BIOS A and Sorvall BIOS A Heavy Duty ..A–1 Table A–2: Directives, Standards and Guidelines Sorvall BIOS A and Sorvall BIOS A
Heavy Duty ......................................................................................................................... A–2
Table A–3: Refrigerants used in the Sorvall BIOS A and Sorvall BIOS A Heavy Duty ...............A–2
Table A–4: Mains Supply ....................................................................................................A–3
Table A–5: Accessories ......................................................................................................A–3
Table B–1: Items supplied for rotor .....................................................................................B–1
Table B–2: F6-10x1000 mL with 1000 mL Bottles,PPCO ....................................................B–2
Table C–1: Chemical Compatibility....................................................................................C–11
v

Preface

Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
Warranty.

Items Supplied

The centrifuges are supplied without a rotor. The items supplied with a rotor are listed in “Rotors” on page B–1.
Article No. Item Quantity
Centrifuge 1
75007698 Thermo Scientific Sorvall BIOS A,
200, 208, 220, 230, 240 V ±10%, 60 Hz, Single phase Japan only: 200 V ±10%, 50 Hz
75007699 Thermo Scientific Sorvall BIOS A,
380, 400, 415 V ±10%, 50/60 Hz, 3-phase
75007700 Thermo Scientific Sorvall BIOS A Heavy Duty,
200, 208, 220, 230, 240 V ±10%, 60 Hz, Single phase Japan only: 200 V ±10%, 50 Hz
Power Supply Cable 1
For Single phase units with 200, 208, 220, 230, 240 V
NEMA 6-50P 50A, 250 V
(Permanently connected with the device at the factory)
For 3-phase units with 380, 400, 415 V
20190369 IEC60309 32A-6h 5 pin red (3P+N+PE),
220/380 V; 230/400 V; 240/415 V
20280119 Bubble Level 1
Instruction Manual 1

Table Preface–1: Items Supplied

If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
vi
Preface

Intended Use for Sorvall BIOS A and Sorvall BIOS A Heavy Duty

This centrifuge is designed to separate sample mixtures of different densities like chemicals, environmental samples and other
non-human body samples.

Associated Rotors and Accessories

The Thermo Scientific™ Sorvall™ BIOS A and Thermo Scientific Sorvall BIOS A Heavy Duty centrifuge can be operated with
the Thermo Scientific™ Fiberlite™ F6-10x1000 LEX rotor. For more details on accessories refer to “Rotors” on page B–1 and
“Accessories” on page A–3.

Precautions

Signal Word and Color Degree of Hazard
WARNING
CAUTION
NOTICE

Table Preface–2: Signal Words and Colors

WARNING NOT FOLLOWING THESE PRECAUTIONS CAN LEAD TO HAZARDOUS SITUATIONS THAT, IF NOT AVOIDED, COULD
RESULT IN DEATH OR SERIOUS INJURY.
Observe the safety instructions. Not following these instructions can cause damage.
The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with
fatal consequences.
The centrifuge must be operated by trained personnel only.
CAUTION
WARNING
Indicates a hazardous situation that, if not avoided, could result in death or serious injury.
Indicates a hazardous situation that, if not avoided, could result in minor or moderate injury.
Indicates information considered important, but not hazard-related.
Due to air friction the temperature of rotor may raise significantly while the centrifuge is spinning. Refrigerated
units have limitations in cooling capabilities. Displayed and set temperature can deviate from sample temperature.
Sample temperature might exceed critical temperature of your application.
The magnets built into the rotors can have a negative effect on active implants, such as cardiac pacemakers. The
magnets are mounted on the bottom of the rotor.
Always keep a distance of 20 cm between the rotor and the active implant, as the product generates permanent
magnetic fields. The magnetic field strength at a distance of 20 cm is less than 0.1 mT, so there should be no
interference.

Set Up Conditions

Plug the centrifuge only into sockets which have been properly grounded.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge.
Do not place any dangerous substances within this safety zone.
Set up in a well-ventilated environment, on a horizontally leveled and rigid surface with adequate load-bearing capacity.
vii
The mains plug must be freely accessible at all times.
To shut down the centrifuge: Press the STOP key. Turn off the centrifuge at the main switch. Pull out the power supply
plug. In an emergency disconnect the power supply.
Preface

Preparation

It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory Biosafety
Manual” of the World Health Organization (WHO) and the regulations in your country.
Do not make any changes to the mechanical components of the centrifuge or rotor. Do not replace mechanical
components.
Do not touch the electronic components of the centrifuge. Do not make any changes to the electronic components. Do not
replace electronic components.
Use only a properly installed rotor. Follow the instructions in section “Install a Rotor” on page III–2.
Do not use rotors or accessories which show any signs of removed protective coating, corrosion or cracks. Contact
customer service for further advice or inspections.
Use only with rotors which have been loaded properly.
Never overload the rotor.
Always balance the samples.
Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific.
Make sure the rotor is locked properly into place before operating the centrifuge.
Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary while it
is running.

Hazardous Substances

Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and the centrifuge
chamber have to be cleaned thoroughly.
Do not centrifuge explosive or flammable materials or substances.
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone
environment.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO) and
any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the “Laboratory Biosafety
Manual” of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the internet page of the
World Health Organization (www.who.int) for the “Laboratory Biosafety Manual”. For materials in a higher risk group, extra safety
measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures have
to be taken (“Disinfection” on page IV–5).
Extreme care should be taken with highly corrosive substances which can cause damage and impair the mechanical
stability of the rotor. These should only be centrifuged in fully sealed tubes.
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.
viii
Preface

Operating

Never use the centrifuge if parts of its casing is damaged or missing.
Do not move the centrifuge while it is running.
Do not lean on the centrifuge.
Do not place anything on top of the centrifuge during a run.
Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed on the
touchscreen.
The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g. during a
power failure (“Mechanical Emergency Door Release” on page V-1).
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bottle crash, the centrifuge is not aerosol-tight.
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.

Maintenance

The centrifuge housing is not to be opened by the operator.
ix
Preface

Symbols used on the Centrifuge

This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to hazards from sharp items.
Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to hazards from closing mechanical parts.
Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol refers to information on hazards, described within the manual.
Observe the information contained in the instruction manual to keep yourself and your environment safe.
This symbol demands to disconnect mains before transporting or servicing the centrifuge.
This symbol shows the direction of rotation.

Table Preface–3: Symbols used on the centrifuge

x
Preface

Symbols used in the Manual

This symbol refers to general hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your environment safe.

Table Preface–4: Symbols used in the manual

xi

Transport and Set Up

THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
Transport and Set Up
I
CAUTION
Make sure that the centrifuge and the packaging is not damaged. Speak to the shipping company and
Thermo Fisher Scientific immediately if damage is found.

1. Location

WARNING
CAUTION
Operate the centrifuge only inside a room.
The set-up location must fulfill these requirements:
Keep a safety zone of minimum 30 cm (Figure I–1 on page I–2, green area) around the centrifuge.
Persons and hazardous substances must be kept out of this safety zone while centrifuging.
Risk of impact.
The centrifuge can crush into objects and persons in a radius of 30 cm when spinning.
Keep a safety zone of 30 cm around the centrifuge for safe operation.
Make sure that no one is in the safety zone while the centrifuge spins.
UV rays decrease the stability of plastics.
Do not expose the centrifuge, rotors and plastic accessories to direct sunlight.
The supporting structure must:
» be stable and free of resonance,
» be applicable for horizontal setup of the centrifuge,
» hold the weight of the centrifuge.
Do not expose the centrifuge to heat and strong sunlight.
The set-up location must be well-ventilated at all times.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
I–1
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
Transport and Set Up

Figure I–1: Centrifuge clearance, example with barcode scanner

2. Transporting

WARNING
CAUTION
NOTICE
NOTICE
Use a forklift to lift a centrifuge that is fixed on a palette.
Impact can damage the centrifuge.
Never stand before a moving centrifuge to stop it.
If the centrifuge is moved on an uneven surface, it can get faster due to its own weight.
An impact of the centrifuge can lead to serious injuries.
Always remove the rotor before you transport the centrifuge.
If you do not remove the rotor you can damage the centrifuge drive or drive shaft.
Dispose the centrifuge packaging.
Assign a shipping company for the transport.
Inform the customer service about the transport.
Send the centrifuge upright and if possible in packaging.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
I–2
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

3. Setting Up

Transport and Set Up
CAUTION
Make sure that the items supplied are complete (“Items Supplied” on page vi).
If the items supplied are not complete, please speak to Thermo Fisher Scientific.
Do not push at the touchscreen.
Due to the weight of the centrifuge it must be controlled by two or more people when rolling of the pallet. Do
not stand in front of the centrifuge when rolling it down the ramp.
The centrifuge has four steering casters that must be parallel, so that the centrifuge can be moved from
the pallet.
Necessary Tools
Graphic Item Quantity
Wrench (30 mm) 2
Socket wrench with nut (19 mm) 1
Screwdriver (torque T30)
Screwdriver (torque T20)

Table I–1: Overview of necessary tools for setting up

1
1

Setting Up

1. Remove the wooden plank on the backside of the pallet. The backside of the pallet is the side with the bevels.
2. Screw both rails on the bevels. Make sure that you position them not at the edge of the pallet.
3. Remove the metal pipes
a. Remove the 4 screws from the downside of the pallet.
b. Lift the centrifuge by turning down the 4 centrifuge feet until the metal pipes can be removed.
c. Remove the metal pipes.
4. Lower the centrifuge by lifting the 4 centrifuge feet.
Make sure that the 4 centrifuge feet are entirely turned up to the bottom of the centrifuge.
5. Move the centrifuge from the pallet.
6. Move the centrifuge to the location, where you want to operate it.
7. Turn down the 4 centrifuge feet until all 4 casters are not in contact with the ground. All 4 centrifuge feet need to have
firm contact to the ground (“Level the Centrifuge” on page I–4). The maximum height for setting up is 9.5 cm (3.7 inch)
measured from the ground to the bottom of the centrifuge.
8. To fasten the 4 centrifuge feet you have to fasten their two lock nuts. The lower lock nut has to be fastened gently down
against the foot. The upper lock nut has to be fastened up against the centrifuge bottom.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
I–3
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

Level the Centrifuge

Transport and Set Up
CAUTION
If you do not level the centrifuge, the centrifuge can crash because of imbalance.
If you move the centrifuge, you must level it again.
Do not move the centrifuge with a rotor attached to the drive shaft because damage can occur to the drive.
Do not put anything below the centrifuge feet to level the centrifuge.
Level the centrifuge as follows:
1. Put the bubble level on the top of the drive shaft in the centrifugation chamber.
2. Adjust the centrifuge feet until the bubble in the level is fully in the circle mark.
3. Turn the drive shaft with the bubble level around a full turn.
If 50% of the bubble stays in the circle mark, the centrifuge is leveled. If more than 50% of the bubble is out of the circle
mark, the centrifuge must be leveled again.
Excellent Acceptable Unacceptable

Figure I–2: Position of the bubble in the water level

4. To fasten the 4 centrifuge feet you have to fasten their two lock nuts. The lower lock nut has to be fastened gently down
against the foot. The upper lock nut has to be fastened up against the centrifuge bottom.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
I–4
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

4. Mains Connection

Transport and Set Up
NOTICE

Figure I–3: Mains connection on the backside of the centrifuge

1. Turn off the power supply switch located on the right side (pull the switch handle towards the front of the centrifuge).
Connect the centrifuge into grounded electrical sockets only.
2. Make sure that the cable specification agrees with the safety standards of your country.
3. Make sure that the voltage and frequency are the same as the figures on the rating plate.

5. Storage

WARNING
Before storing the centrifuge and the accessories it must be clean and if necessary disinfected and decontaminated.
Centrifuge, rotors and accessories have to be fully dry before storage.
Keep the centrifuge in a clean, dust-free location.
Keep the centrifuge on its rubber feet and not on its casters.
Do not store the centrifuge in direct sunlight.
When you remove the centrifuge and accessories from use, clean and if necessary disinfect or decontaminate
the full system. If you are not sure speak to the Thermo Fisher Scientific customer service.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
I–5
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.

6. Shipping

Transport and Set Up
WARNING
Before shipping the centrifuge and accessories you must clean and if necessary, disinfect or decontaminate
the full system. If you are not sure speak to the Thermo Fisher Scientific customer service.
Before shipping the centrifuge:
The centrifuge must be clean and decontaminated.
You must confirm the decontamination with a decontamination certificate.
THE ACTIVITIES DESCRIBED IN THIS CHAPTER ARE FOR SERVICE TECHNICIANS.
I–6
ThermoScientific Centri-Touch User Interface
ThermoScientific Centri-Touch User Interface
II
➀
➁
➂
Actual status information
Set values
Settings and navigation
II–1
ThermoScientificCentri-Touch User Interface

Status

The upper part of the touchscreen displays the centrifuge status.
The remaining time is indicated while centrifugation is in progress. The progress bar shows the actual phase of the
centrifugation.
➀
➁
➂
➃
Status that can be displayed
Ready Centrifugation can be started.
Door open Centrifuge door is open.
Door moving Door is automatically closing or opening.
Error An error has occurred.
Canceled Centrifugation has been stopped manually.
Complete Centrifugation has been successfully completed.
Pretempering completed
No rotor No rotor is identified in the centrifuge.
Status: In the Time mode, the remaining time for the centrifugation process is shown here. In the Hold mode, the elapsed time is displayed.
Progress: The curve diagram is divided into the sections acceleration, centrifugation and deceleration.
Speed: The current speed of the rotor is shown here.
Temperature: The temperature in the centrifugation chamber is shown here.
Pretempering has been successfully completed.
II–2
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