Thermo Scientific RC-4 Instruction Manual

Thermo Scientific
Sorvall RC-4

Instruction Manual

20057791-f • 02 / 2021

Visit us online to register your warranty:

thermofisher.com/labwarranty

EE Conformity

WE

This product is subject to the regulations of the EU Waste Electrical & Electronic
Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:

T

Table of Contents

Preface ....................................................................................................................................... iii

Scope of Supply .......................................................................................................................iii

Intended Purpose ......................................................................................................................iv

Accident Prevention .................................................................................................................iv

Precautions ................................................................................................................................iv

Chapter 1 Introduction and Description ...............................................................................................1-1

Characteristics of the Sorvall RC-4 .......................................................................... 1-2

Technical Data ........................................................................................................ 1-3

Directives, Standards and Guidelines ...................................................................................... 1-4

Functions and Features ............................................................................................................ 1-4

Mains Supply ........................................................................................................... 1-5

Refrigerants ..............

Rotor Selection .........

Chapter 2 Before Use ...............................................................................................................................2-1

Before Setting up ..................................................................................................... 2-2

Setting up ................................................................................................................ 2-2

Aligning the Centrifuge ........................................................................................... 2-4

Transporting the Centrifuge .................................................................................... 2-4

Location .................................................................................................................. 2-4

Mains Connection ................................................................................................... 2-5

Adjust Voltage and Frequency ................................................................................. 2-6

Storage ..................................................................................................................... 2-6

............................................................................................... 1-6

............................................................................................... 1-6

Chapter 3 Control Panel ..........................................................................................................................3-1

Control Panel .......................................................................................................... 3-2

Keys ......................................................................................................................... 3-2

Chapter 4 Operation .................................................................................................................................4-1

Switch on Centrifuge ............................................................................................... 4-2

Centrifuge Door ..................................................................................................... 4-2

Rotor Installation ..................................................................................................... 4-3

Entering Parameters ................................................................................................. 4-4

Centrifugation ......................................................................................................... 4-8

Temperature Adaptation during Standstill ............................................................... 4-9

Short-term Centrifugation ....................................................................................... 4-9

Removing the Rotor ................................................................................................ 4-9

Turning off the Centrifuge .................................................................................... 4-10

Audible Alarm ....................................................................................................... 4-10

Key Switch ............................................................................................................. 4-11

Thermo Scientific Sorvall RC-4 i

Contents

Chapter 5 Maintenance and Care .........................................................................................................5-1

Cleaning Intervals .................................................................................................... 5-2

Cleaning .................................................................................................................. 5-2

Cleaning the Venting Slots ...................................................................................... 5-3

Disinfection ............................................................................................................. 5-3

Decontamination ..................................................................................................... 5-4

Service of Thermo Fisher Scientific .......................................................................... 5-5

Chapter 6 Troubleshooting .....................................................................................................................6-1

Mechanical Emergency Door Release ...................................................................... 6-2

Troubleshooting by User ......................................................................................... 6-3

When to contact a Service Technician ..................................................................... 6-5

Chemical Compatibility Chart ..............................................................................A-1

ii Sorvall RC-4 Thermo Scientific

P

Preface

Before starting to use the centri
instructions.

The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.

Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the seller's warranty.

Scope of Supply

Article Number Quantity Check

20057791 Instruction Manual 1

50127698 CD with Manual 1

fuge, read through this instruction manual carefully and follow the

Sorvall RC-4 Centrifuge 1

Power Supply Cable 1

If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.

This symbol refers to general hazards.

CAUTION means that material

WARNING means that injuries or material damage or contamination could occur.

This symbol refers to biological hazards.

Observe the information contained in the instruction manual to keep yourself and your
environment safe.

Thermo Scientific Sorvall RC-4 iii

damage could occur.

P

g

cm

3

---------- -

Intended Purpose

Intended Purpose

• This centrifuge is a laboratory product designed to separate components by generation of a

relative centrifugal force. It separates human samples (e.g. blood, urine and other body fluids)
collected in appropriate containers, either alone or after addition of reagents or other additives.

• Laboratory centrifuge for the application in large laboratories, blood banks, and other the sector

of the pharmaceutical and bio-technological industry.

• The centrifuge is to be used for separating materials of different density or particle size

suspended in a liquid.
Maximum sample density at maximum speed: 1.2

Accident Prevention

Prerequisite for the safe operation of the Sorvall RC-4 is a work environment in compliance
with standards, directives and trade association safety regulations and proper instruction of
the user.

The safety regulations contain the following basic recommendations:

Precautions

In order to ensure safe operation of the Sorvall RC-4, the following general safety regulations
must be followed:

• Maintain a radius of at least 30 cm around the centrifuge.

• Implementation of special measures which ensure that no one can approach the

centrifuge for longer than absolutely necessary while it is running.

• The mains plug must be freely accessible at all times. Pull out the power supply plug or

disconnect the power supply in an emergency.

• The centrifuge should be operated by trained specialists only.

• The centrifuge is to be used for its intended use only.

iv Sorvall RC-4 Thermo Scientific

• Plug the centrifuge only into sockets which have been properly grounded.

P

Precautions

• Do not move

the centrifuge while it is running.

• Do not lean on the centrifuge.

• Use only rotors and accessories for this centrifuge which have been approved by Thermo

Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.

• Do not use rotors which show any signs of corrosion and / or cracks.

• Do not touch the mechanical components of the rotor and do not make any changes to

the mechanical components.

• Use only with rotors which have been properly installed. Follow the instructions in

section “Rotor Installation” on page 4-3.

• Use only with rotors which have been loaded properly. Follow the instructions given in

the rotor manual.

• Never overload the rotor. Follow the instructions given in the rotor manual.

• Never open the lid until the rotor has come to a complete stop and this has been

confirmed in the display.

• The lid emergency release may be used in emergencies only to recover the samples from

the centrifuge, e.g. during a power failure (see section “Mechanical Emergency Door

Release” on page 6-2).

• The performance of the gas pistons might diminish after some time. Check the gas

piston as described in “Centrifuge Door” on page 4-2.

• Never use the centrifuge, if parts of its cover panels are damaged or missing.

• Do not touch the electronic components of the centrifuge or alter any electronic or

mechanical components.

• Please observe the safety instructions.

Please pay particular attention to the following aspects:

• Location: Well-ventilated environment, set-up on a level and rigid surface with

adequate

load-bearing capacity.

• Rotor installation: Make sure the rotor is locked properly into place before operating the

centrifuge.

• Especially when working with corrosive samples (salt solutions, acids, bases), the

accessory parts and vessel have to be cleaned carefully.

• Always balance the samples.

Thermo Scientific Sorvall RC-4 v

P

Precautions

Centrifuging hazardous substances:

• Do not centrifuge explosive or flammable materials or substances which could react

violently with one another.

• The centrifuge

is neither inert nor protected against explosion. Never use the centrifuge

in an explosion-prone environment.

• Do not centrifuge inflammable substances.

Remaining risk: Improper use can cause damages, contamination and injuries with
fa-tal consequences.

• Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms

without suitable safety precautions.

When centrifuging microbiological samples from the Risk Group II (according to the

“Laboratory Biosafety Manual” of the World Health Organization (WHO)),

aerosol-tight biological seals have to be used.

For materials in a higher risk group, extra safety measures have to be taken.

• If toxins or pathogenic substances have gotten into the centrifuge or its parts,

appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3).

Remaining risk: Improper use can cause damages, contamination and injuries with
fatal consequences.

• Highly corrosive substances which can cause material damage and impair the mechanical

stability of the rotor, should only be centrifuged in corresponding protective tubes.

WARNUNG

If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the
area immediately.

vi Sorvall RC-4 Thermo Scientific

1

Introduction and Description

Contents

• “Characteristics of the Sorvall RC-4” on page 1-2

• “

Technical Data” on page 1-3

• “Directives, Standards and Guidelines” on page 1-4

• “Functions and Features” on page 1-4

• “Mains Supply” on page 1-5

• “Refrigerants” on page 1-6

• “Rotor Selection” on page 1-6

Thermo Scientific Sorvall RC-4 1-1

1

Introduction and Description

Characteristics of the Sorvall RC-4

Characteristics of the Sorvall RC-4

The Sorvall RC-4 is a large volume, refrigerated universal centrifuge.

The Sorvall RC-4 is designed for large volume, high throughput laboratory needs. The micro test plates
and tubes can be centrifuged with the unique double rectangular bucket.

The Sorvall RC-4 is optimized for the automatic laboratory processes in clinical central labs.

The Diagnostik™ rotor allows the centrifuge commercial sample racks directly.

The Sorvall RC-4 is also a compact centrifuge for the separation of blood components. A special wind
shield rotor allows to process up to eight quintuple blood bag systems.

The Sorvall RC-4 is equipped with various safety features:

• The housing and rotor chamber consist of steel plate, the interior of armor steel, while the front

panel is made of

• The door is equipped with a view port and a lock.

high-impact resistant plastic.

• The centrifuge door can only be opened while the centrifuge is switched on and the rotor has come

to a complete stop. The centrifuge cannot be started until the door has been closed properly.

• The integrated rotor detection systems ensures that no inadmissible speed settings can be

preselected.

• Electronic imbalance detection, SMARTspin.

• Door emergency release: For emergencies only, e.g. during power failures

(see “Mechanical Emergency Door Release” on page 6-2).

1-2 Sorvall RC-4 Thermo Scientific

Technical Data

The technical data of the Sorvall RC-4 is listed in the following table.

Table 1 - 1. Technical Data

Feature Value

1

Introduction and Description

230 V 200 V 120 V

Technical Data

Environmental Conditions

Environmental Conditions
during Storage and Shipping

Permissible Ambient Temperature

Overvoltage Category II II II

Pollution Degree 2 2 2

Heat Dissipation 9200 BTU/h 9200 BTU/h 6300 BTU/h

IP 20 20 20

Run Time Unlimited Unlimited Unlimited

Max Speed n

Min Speed n

Maximum RCF-Value at n

Maximum kinetic Energy <63.4 kJ <63.4 kJ <63.4 kJ

max

min

max

-Use in interior Spaces

-Altitudes of up to 2,000 m above Sea Level

-Max. relative Humidity 80 % up to 31 °C; Decreasing linearly up to 50 %
Relative Humidity at 40 °C.

Temperature:

-10 °C to +55 °C
Humidity: 15

+2 °C

10000 rpm
(depending on rotor)

300 rpm 300 rpm 300 rpm

15317 x g 15317 x g 15317 x g

% to 85 %

to +40 °C

Temperature:

-10 °C to +55 °C
Humidity: 15 % to 85 %

+2 °C

to +40 °C

10000 rpm
(depending on rotor)

Temperature:

-10 °C to +55 °C
Humidity: 15 % to 85 %

+2 °C

to +40 °C

10000 rpm
(depending on rotor)

Noise Level at maximum Speed <61 dB(A) <61 dB(A) <61 dB(A)

Temperature Setting Range

Dimensions

Height
Width
Depth

Weight without Rotor 286 kg 286 kg 286 kg

-9 °C to +40 °C -9 °C to +40 °C -9 °C to +40 °C

960 mm
680 mm
720 mm

960 mm
680 mm
720 mm

960 mm
680 mm
720 mm

Thermo Scientific Sorvall RC-4 1-3

1

Introduction and Description

Directives, Standards and Guidelines

Directives, Standards and Guidelines

Ta

ble 1-2. Directives, Standards and Guidelines

Region

Europe

USA & Canada

Directive

2006/42/EC Machinery

Protective goals of 2014/35/EU Low Voltage

2014/30/EU Electromagnetic Compatibility (EMC)

2011/65/EU RoHS

Directive on the Restriction of the use of certain

Hazardous Substances in electrical and electronic

equipment

cCSAus certified

Functions and Features

The following table gives an overview of the important functional and performance characteristics of
the Sorvall RC-4.

Table 1-3. Functions and Features

Standard

EN 61010-1

EN 61010-2-020

EN 61326-1

EN 61000-6-2

UL 61010-1

CAN/CSA-C22.2 No. 61010-1

IEC 61010-2-020

Component / Function Description / Features

Structure / Housing Galvanized Steel Chassis with armored Plating

Chamber Stainless Steel

Drive Induction Drive without Carbon Brushes

Keys and Display Easy-to-clean Keypad and Display Surface

Control Microprocessor controlled with Easycontrol II

Internal Memory The most recent Data is saved

Functions RCF-selection, Temperature Regulation during Standstill, Pre-temp,

and PULSE

Acceleration / Braking Profiles 9 Acceleration and 9 Braking Curves

Rotor Recognition Automatic

Imbalance Detection SMARTspin Electronic, contingent on Rotor and Speed

Door Lock Motor assisted Door Looking

1-4 Sorvall RC-4 Thermo Scientific

Function Feature

1

Introduction and Description

Mains Supply

Program Display

Acceleration / Braking Profiles 1 = slow, ... 9 = fast Acceleration / Deceleration Curve

Speed Selection Adjustable from 300 rpm to10,000 rpm in 10 rpm increments

RCF Selection After actuating the RCF Switch Key, the RCF Value can be entered.

Time Selection Adjustable in Seconds up to 9 min 59 sec,

Run Time Display in "quick run"
Mode

Temperature Selection Adjustable from -9°C to +40°C, in 1°C Increments.

End of Run The Speed Display will read End.

Door Opening Automatic unlocking via Key

Start Key

Stop Key

Free Program Selection

Operating Memory

1...9

Memory Capacity for set Values
PULSE-Mode

in the Range of 10 min up to 99 min

in Minutes, "hold"-Mode: Continuous Operation

In Seconds Increments

(Unlocking in Case of Power Failure:
see “Mechanical Emergency Door Release” on page 6-2)

PULSE-Mode Pressing the Key activates maximum Acceleration up to the maximum

Diagnostic Messages Alternating Display rotor /maximum Speed or RCF (Speed selected exceeds

Mains Supply

The following table contains an overview on the electrical contact data of the Sorvall RC-4. This data is
to be taken into consideration when selecting the mains connection socket.

Table 1 - 4. Electrical Connection Data

Cat.

75004473 230 V 50 Hz 12 A 2.7 kW 16 AT 16 AT

75004477 120 V 60 Hz 16 A 1.8 kW 16 AT 20 AT

Mains
Voltage

permissible Speed of Rotor; upon Key release Centrifuge stops with maximum
Deceleration Power.

max. Speed of the Rotor)

Door has not been lifted off the Lock during Opening: Display Lift Lid (Manual
Lifting of Door required)

General Centrifuge Malfunction (Error Messages with ERROR Codes,
see“Troubleshooting by User” on page 6-3)

Frequency Rated

Current

Power
Consumption

Equipment fuse Building fuse

75004481 200 V 50 / 60 Hz 14.5 / 16 A 2.7 kW 16 AT 20 AT

75004481 208 V 60 Hz 14.5 A 2.7 kW 16 AT 20 AT

75004481 230 V 60 Hz 14.5 A 2.7 kW 16 AT 20

Thermo Scientific Sorvall RC-4 1-5

AT

1

Introduction and Description

Refrigerants

Refrigerants

Table 1-5. Refrigerants

Article No. Centrifuge Refrigerant Quantity

75004473

7500

4477

75004481

Contains fluorinated greenhouse gases in a hermetically sealed system.

Rotor Selection

The Sorvall RC-4 is supplied without a rotor.

Various Thermo Scientific rotors are available to choose from.

LH-4000

with round buckets 1000

Double rectangular bucket DoubleSpin

LH-4000W

with round buckets 1000

Double rectangular bucket DoubleSpin

Thermo Scientific Sorvall RC-4

Thermo Scientific Sorvall RC-4

Thermo Scientific Sorvall RC-4

TM

TM

R-134A

R-134A

R-134A

2 x 250

2 x 250

Pressure

1

kg 21 bar 1430

1 kg 21 bar 1430 1.43 t

1 kg 21 bar 1430 1.43 t

75006475

75006477

75006478

75006476

75006477

75006478

GWP

CO2e

1.43 t

with double blood bag bucket

BIOshield 4 x 250

HIGHPlate 5 x 5 Platten

Diagnostik

HIGHConic 6 x 50

LAC-250 6 x 250

TM

75006436

75006435

75006444

75006480

75003057

75006483

The technical data of the rotors and the corresponding adapters and reduction sleeves for various

ommercially available containers can be found in the corresponding rotor operating manuals.

c

For more information visit our website at: http://www.thermofisher.com

1-6 Sorvall RC-4 Thermo Scientific

2

Before Use

Contents

• “Before Setting up” on page 2-2

• “Setting up” on page 2-2

• “Location” on page 2-4

• “Mains Connection

• “Storage” on page 2-6

” on page 2-5

Thermo Scientific Sorvall RC-4 2-1

2

Rails

Screws

Before Use

Before Setting up

Before Setting up

1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately, if any damage
is discovered.

2. Remove the packaging.

Setting up

3. Check the or
If the order is incomplete, please contact Thermo Fisher Scientific.

Note The centrifuge is screwed onto the pallet with four screws.

1. Place the pallet with the centrifuge so that you have atleast 2 m room in front of the pallet.

2. Release the rails from the pallet.

3. Screw the rails onto the pallet in front of the centrifuge.

der for completeness (see “Scope of Supply” on page iii).

WARNING The centrifuge operated with a high kinetic energy. Do not operate the
centrifuge wi
Do not operate the centrifuge on its castors.

thout a 30 cm (12 inch) safety zone around the centrifuge.

2-2 Sorvall RC-4 Thermo Scientific

2

Threaded Rod

Lock Nut

Safety Bracket

Locking Stabilizer

Threaded Nut

Threaded Nut

Before Use

Setting up

4. Screw the threaded rods with the threaded nuts as far up as possible. Otherwise they might get
stuck when rolling the centrifuge off the pallet.

5. Remove the locking stabilizers.

6. Check that the steering casters point to the back.

7. Use several people and other means in order to roll the centrifuge off the pallet.

WARNING Due to the centrifuges weight, it must be controlled by several people when
rolling of the pallet. Do not stand in front of the c

entrifuge when rolling down for the

centrifuge might run you over.

8. Once the centrifuge is in its final position mount the enclosed locking stabilizers.

Thermo Scientific Sorvall RC-4 2-3

9. Screw the threaded nuts down.

10. Place the locking stabilizers centrally under the threaded rods and level the centrifuge. All the
locking stabilizers should carry uniformly (check by a tilt test). The casters are some 5-15 mm off
the ground.

2

Before Use

Aligning the Centrifuge

Aligning the Centrifuge

11. Switch on the centrifuge.

12. Open the centrifuge.

13. Put the enclosed water level on the drive spindle.

14. Use an open-end wrench to level the centrifuge and to raise and lower it.

CAUTION If the centrifuge is not level, imbalances can occur and the centrifuge can be
damaged.
The horizontal leveling of the centrifuge must be checked every time after moving it to a
different location.
The supporting structure must be suitable for horizontal setup of the centrifuge.
Do not place anything under the feet to level the centrifuge.

15. Remove the water level when the centrifuge is leveled.

16. Be sure that the four locking stabilizers stand safe and do not jolt.

17. Fasten the lock nuts by screwing them up as far as possible.

18. Screw the safety bracket onto the locking stabilizers.

19. The final assembly of the safety brackets is carried out after leveling.

Transporting the Centrifuge

• Use a forklift to lift the centrifuge.

• The centrifuge can be damaged by impacts.

• Transport the centrifuge upright and if at all possible in packaging.

Note The packaging is an one-way packaging. Contact a shipping company for the transport.

WARNING Always remove the rotor before moving the centrifuge.

Location

The centrifuge should only be operated indoors.

The set-up location must fulfill the following requirements:

• A safety zone of at least 30 cm must be maintained around the centrifuge.

People and hazardous substances must be kept out of the safety zone while centrifuging.

• The supporting structure must be stable and free of resonance.

• The supporting structure must be suitable for horizontal setup of the centrifuge.

2-4 Sorvall RC-4 Thermo Scientific

• The centrifuge should not be exposed to heat and strong sunlight.

Mains Plug

WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

• The set-up location must be well-ventilated at all times.

Mains Connection

2

Before Use

Mains Connection

Figure 2-1. Mains Connection

1. Turn off the power supply switch located on right side (press “0”).

2. Plug the centrifuge into grounded electrical

sockets only.

3. Check whether the cable complies with the safety standards of your country.

4. Make sure that the voltage and frequency correspond to the figures on the rating plate.

5. Establish the connection to the power supply with the connecting cable.

Thermo Scientific Sorvall RC-4 2-5

2

Before Use

Adjust Voltage and Frequency

Adjust Voltage and Frequency

It is necessary that you set the voltage and frequency during setup.

1. Pull out the power supply plug.

CAUTION Remove the power supply plug in order to prevent damage to the centrifuge.

2. Set the switches on the back as shown in the table.

3. Connect the centrifuge to the power supply again.

Storage

• Before storing the centrifuge and the accessories,

and decontaminated.

• Store the centrifuge in a clean, dust-free location.

• Be sure to place the centrifuge on its feet.

• Avoid direct sunlight.

2-6 Sorvall RC-4 Thermo Scientific

it must be cleaned and if necessary, disinfected

3

Control Panel

Contents

• “Control Panel” on page 3-2

• “Keys” on page 3-2

Thermo Scientific Sorvall RC-4 3-1

3

Acceleration Curve

Braking Curve

Speed / RCF Value

Run Time

Temperature

PULSE

OPEN

START

STOP

Optional Accessory

Program

Key Switch

Program Selection

Program Memory

Pre-warming

Bucket Selection

Toggle Key

or Pre-cooling

Control Panel

Control Panel

Control Panel

The control panel contains the keys and displays of the centrifuge (only the power switch is located on
the right side of the device). All parameters can be selected and changed during operation.

Keys

The keys allow user intervention for controlling the operating mode as follows:

Key Display contents

Start

Stop

Open Door

Pulse

UP

Bucket

Pre-Temp

Changing the
Display Mode

Normal start of the centrifuge.

End run manually.

Automatic release (possible only when device is switched on); Emergency
release (see “Mechanical Emergency Door Release” on page 6-2).

By pressing the key the centrifuge starts immediately and accelerates
up to the end speed. Releasing the key initiates a stopping process at the
highest braking curves.

By pressing the key you change the value in the display.

Use the key in order to have all available bucket types displayed in
succession.

By pressing the key you start the pre-temp function of the centrifuge.

Use the key to change the display mode.

3-2 Sorvall RC-4 Thermo Scientific

SAVE

Press the key to save a program.

By pressing the key up and down you change the value in the
display. If you hold the key pressed, the display changes continuously at
first slowly and after a few seconds at an accelerated pace to the higher or
lower values. By pressing the key briefly, you increase or decrease the
speed in one step. When pressing both arrows the cursor moves to the left.

4

Operation

Contents

• “Switch on Centrifuge” on page 4-2

• “Centrifuge Door” on page 4-2

• “Rotor Installatio

• “Entering Parameters” on page 4-4

• “Centrifugation” on page 4-8

• “Temperature Adaptation during Standstill” on page 4-9

• “Short-term Centrifugation” on page 4-9

• “Removing the Rotor” on page 4-10

• “Turning off the Centrifuge” on page 4-10

• “Audible Alarm” on page 4-10

• “Key Switch” on page 4-11

n” on page 4-3

Thermo Scientific Sorvall RC-4 4-1

4

Operation

Switch on Centrifuge

Switch on Centrifuge

1. Turn on the power switch on the right side of the device.
The centrifuge performs a self-check of its software. Subsequently, the following readings will be
displayed. The display shows the values from the last run.

Centrifuge Door

WARNING The performance of the gas pistons might diminish after some time. Do not

place you hand in the

Check the gas pistons as described below:

• Open the centrifuge approximately 10 cm.

door opening when the door is not totally open.

Door Opening

The door can only be opened when the centrifuge is turned on.

1. Press the key.

Close Door

1. Close the door by pressing down on it lightly in the middle or on both sides of it. Two locks close

• If the door remains in this position, the gas

• If the door moves downwards, the gas pistons need to be replaced.

WARNING Do not reach into the crack between the door and the housing. The door is
drawn shut automatically.

Use the emergency release only for malfunctions and power failures
(see “Mechanical Emergency Door Release” on page 6-2).

the door completely.

Note The door should audibly click into place.

pistons are working fine.

WARNING Do not reach into the crack between the door and the housing. The door is
drawn shut automatically.

Use the emergency release only for malfunctions and power failures
(see “Mechanical Emergency Door Release” on page 6-2).

CAUTION Do not slam the door.

4-2 Sorvall RC-4 Thermo Scientific

Rotor Installation

The approved rotors for the Sorvall RC-4 are listed in section “Rotor Selection” on page 1-6. Use only
the rotors

Proceed as follows:

1. Open the door of the centrifuge and if necessary, remove any dust, foreign objects or residue

2. Hold the rotor over the centrifuge spindle.

3. Let the rotor slide slowly down the centrifuge spindle.

and accessories from this list in the centrifuge.

CAUTION Unapproved or incorrectly combined accessories can cause serious damage to
the centrifuge.

the chamber.

from
Thread and O-ring must be clean and undamaged.

4

Operation

Rotor Installation

CAUTION Do not force the rotor onto the centrifuge spindle.

4. Thread the fastening tool into the centrifuge spindle clockwise. Hold the rotor with the other hand
into position.

5. Check if the rotor is properly installed by lifting it slightly on the handle.

WARNING Check for any damage to the rotor. Damaged rotors must not be used.

p the centrifuge spindle area of the rotor clear of objects.

Kee

CAUTION Check that the rotor is properly locked on the centrifuge spindle before each use
by pulling it at its handle.

6. If available, close the rotor with the rotor lid.

Be sure to check all sealings before starting any aerosol-tight applications.
See the information in the rotor instruction manual.

7. Close the centrifuge door.

Thermo Scientific Sorvall RC-4 4-3

4

Operation

Entering Parameters

Entering Parameters

Acceleration Curve

The Sorvall RC-4 offers you a total of 9 acceleration and 9 braking curves with which samples and
gradients can be centrifuged.

After the centrifuge is turned on, the last running profile selected is shown.

1. Press the
Curve number 1 is the slowest and curve number 9 the fastest.

2. The value is stored when the value stops flashing.

below the field acceleration profile to change the acceleration profile.

Braking Curve

1. Press the below the field breaking curve to change the breaking curve.
Curve number 1 is the slowest and curve number 9 the fastest.

2. The value is stored when the value stops flashing.

Preselecting Speed / RCF

1. Press the key below the field speed / RCF value. Next to the display a light shows the actual
unit.

2. The display shows the speed or the RCF value depending on the display setting. Press the to

toggle between the

two modes.

key

3. Enter the desired value by pressing up or down.

The speed can be selected between 300 rpm and 10000 rpm and can be altered by 10 rpm.
The RCF can be set in steps of 1.

4. The value is stored when the value stops flashing.

Note If an extremely low RCF value has been selected, it will be corrected automatically if the
resulting speed is less than 300rpm.

4-4 Sorvall RC-4 Thermo Scientific

4

n

1000

-------------



2

r×

=

t

K

S

20,w

-----------

=

K 253000()n

r

max

r

min

---------





Speed

1000

-------------





2

÷=

Operation

Entering Parameters

Explanation of RCF Value

The relative centrifugal force (RCF) is given as a multiple of the force of gravity g. It is a unitless
numerical value which is used to compare the separation or sedimentation capacity of various
devices, since it is independent of the type of device. Only the centrifuging radius and the speed
come into play in it:

RCF 11.18 × 

r = centrifuging radius in cm

n = rotational speed in rpm

The maximum RCF value is related to the maximum radius of the tube opening.

Remember that this value is reduced depending on the tubes and adapters used.

This can be accounted for in the calculation above if required.

Explanation ofK-Factor

The k-factor is an information on the sedimentation capacity of a rotor. You can calculate the
required sedimentation time of each rotor with this formula:

t = sedimentation time in hours

K = clearing factor of the rotor (K-factor)

S

= Sedimentation factor for sample particles in water at 20 °C in Svedbergs

20,w

The k-factors given in this manual refer to:

r

= bucket bottom, (bottom of cavity of fixed angled rotors)

max

= bucket upper edge, (upper edge of cavity of fixed angled rotors)

r

min

Tubes which have various radii due to adapter or micro plate carriers the k-factor can be calculated
by using this formula:

Thermo Scientific Sorvall RC-4 4-5

Run Time Preselection

5. Press the key below the field run time.

1. Enter the desired value by pressing up or down.
Up to 9 minutes 59 seconds the duration is given exactly to the second.

4

Operation

Entering Parameters

After 10 minutes, the minutes are given.

Continuous Operation

1. Press the key below the field run time.

2. Press the key up or down until the following is shown:

During continuous operation, the centrifuge will continue running until you stop it manually with
the key.

Limited Time Mode

You can limit the run time to 9:59.

1. Press the

key for 1 second.

The display shows:

2. Press the

key below the field speed upwards.

The display shows:

3. Press the

key below the field run time upwards.

You can either choose the limited time mode 0.00 or the standard mode 00.0.

Preselecting the Temperature

Y

ou can preselect temperatures between -9 °C and +40 °C.

1. Press the

key below the field temperature.

2. Enter the desired value by pressing up or down.
The temperature can be set in steps of 1.
The value is stored when the value stops flashing.

4-6 Sorvall RC-4 Thermo Scientific

Pre-warming or Pre-cooling the Centrifuge

For setting the pre-temp value for the centrifuge proceed as follows:

1. Press the key and enter the desired value by pressing up or down.
The display shows:

4

Operation

Entering Parameters

2. Press the

key.

The rotor will be pre-temped for 30 to 60 minutes.

Bucket Selection

Bucket selection is only possible for swing-out rotors.
The bucket code corresponds to the last four digits of the bucket catalog number.
The selected bucket is needed to calculated the RCF-value and control the temperature.

1. Press the key below the field speed / RCF-value until the correct bucket is shown:

2. The value is stored and the display shows the speed or RCF-value.

Setting up a Program

1. Press the key below the field program until the correct number in the program memory is
shown: The display flashes.

2. Press the key.
All displays flash.

1. Enter the program parameters.

2. As described above, please enter braking and acceleration profile, speed / RCF value, run

time,

temperatures, and bucket.

The value that is being changed flashes.

3. Press the key again when all displays are flashing again.
The display with the number of the program memory flashes and all settings are stored.

Thermo Scientific Sorvall RC-4 4-7

4

Operation

Centrifugation

Changing the Settings during a Run

You can change the set values during a run. Press either the key
The value that is being changed flashes. When the new value is
in the display.

Centrifugation

Once the rotor has been properly installed, the main switch has been turned on and the door has been
closed, you can start centrifuging.

Starting Centrifuge Program

Press the key on the control panel. The centrifuge accelerates up to the set value. The time display
shows the remaining run time. If you have selected continuous operation, the time display counts up.

If you have selected a value that is above the maximum speed or RCF-value, the display will flash for
15 seconds between rotor and the maximum value for the installed rotor.

a. Please enter a new value. Within this period, it is possible to accept this value by again pressing

the key. The centrifugation is then continued.

b. After 15 sec. the rotor will be decelerated. Always wait until the rotor has come to a stop

without braking.
By opening and closing the lid you reset the message rotor.
After entering a permissible speed you can start again.

or below the display.

stored the actual value is shown

You cannot open the door as long as the centrifuge is running.

Imbalance Indicator

If a load is imbalanced, this will be indicated at speed higher than approx. 300 rpm. The display shows
the following message:

The run will terminate.

Check the loading and start the centrifuge once again. See the information on proper loading in the
rotor instruction manual. For information on troubleshooting, see section “Troubleshooting by User”
on page 6-3.

4-8 Sorvall RC-4 Thermo Scientific

Stopping the Centrifugation Program

With preset Run Time

Usually the run time is preset and you only have to wait until the centrifuge stops automatically when
the preset time limit expires.

As soon as the speed drops to zero, the message End will appear in the display. By pressing the key,
you can open the door and remove the centrifuge material.

You can also stop the centrifuging program manually at any time by pressing the key.

Continuous Operation

If you selected continuous operation (see “Continuous Operation” on page 4-6), you will have to stop
the centrifuge manually. Press the key on the control panel. The centrifuge will be decelerated at
the designated rate. As soon as the speed drops to zero, the message End will appear in the display. By
pressing the key, you can open the door and remove the sample.

4

Operation

Temperature Adaptation during Standstill

Temperature Adaptation during Standstill

The temperature cannot be adapted until the rotor has been positively identified. For this, the
centrifuge must have

When the rotor is not recognized (door closed and key not yet pressed, speed display 0), the
centrifuge responds by ensuring that the sample cannot freeze regardless of the rotor being used.

accelerated to over 300 rpm. The speed display will then show End.

Short-term Centrifugation

For short-term centrifuging, the Sorvall RC-4 has a PULSE- function.

By holding down the key, spinning will start and continue until the key is let go.

The centrifuge accelerates and brakes at maximum power. Any rpm or RCF entered beforehand is
overridden.

Note The centrifuge accelerates to maximum speed, regardless of which rotor was installed.

Check carefully whether you have to maintain a certain speed for your application.

During the acceleration process, time is counted forwards in seconds. The reading stays displayed until
the centrifuge door is opened.

Thermo Scientific Sorvall RC-4 4-9

4

Operation

Turning off the Centrifuge

Removing the Rotor

To remove the rotor, proceed as follows:

1. Open the centrifuge door.

2. Thread the fastening tool in the centrifuge spindle counter-clockwise. Hold the rotor with the
other hand into position.

3. At the same time, pull the rotor directly upwards with both hands and remove it from the
centrifuge spindle. Make sure not to tilt the rotor while doing this.

Aerosol-tight Rotors

When using an aerosol tight lid, the rotor can only be removed with the lid closed. This is to
protect you and the samples.

Turning off the Centrifuge

o turn off the centrifuge, put the mains switch to “0”.

1. T

Audible Alarm

Error messages are accompanied by an audible alarm. Press any key to silence the audible alarm.

The end of run can also be accompanied by an audible alarm. To switch off this signal proceed as
follows:

1. Press the
The display shows the current setting.

2. Use the key below the field run time until it says ON in the display if the centrifuge should
make an audible alarm after the run.
Use the key below the field run time until it says OFF in the display if the centrifuge should
not make an audible alarm after the run.

key for 1 second.

Note If the message rotor appears in the display the selected speed or RCF-value are to high. If you
press the key the centrifuge accelerates to the shown maximum value.

4-10 Sorvall RC-4 Thermo Scientific

Key Switch

The key can lock some functions of the centrifuge.

Key position Description

4

Operation

Key Switch

A

B

C

All settings can be changed.

The user can change and use all programs in the program memory. PULSE and the pre-temp
function can be used.

The program memory is protected against changes.

The user can use all programs but cannot change them.

PULSE and the pre-temp function can be used.

Program memory and RAM are protected against changes.

All set parameters are blocked, but the actual program can be operated as often as needed
with the control keys ( , , ).

All set values can be seen with the arrow keys.

The selected program cannot be changed.

PULSE and the pre-temp function are locked.

Thermo Scientific Sorvall RC-4 4-11

5

Maintenance and Care

Contents

• “Cleaning Intervals” on page 5-2

• “Cleaning” on page 5-2

• “Disinfection” on page 5-3

• “Decontamination” on page 5-4

• “Service of Thermo Fisher Scientific” on page 5-5

Thermo Scientific Sorvall RC-4 5-1

5

Maintenance and Care

Cleaning Intervals

Cleaning Intervals

For the sake of personal, environmental and material protection, it is your duty to clean and if
necessary, disinfect the centrifuge on a regular basis.

Maintenance Recommended interval

Clean Rotor Chamber Daily or when soiled

Clean Rotor Daily or when soiled

Accessories Daily or when soiled

Cabinet Once per Month

Ventilation holes Every six Months

CAUTION Refrain from using any other cleaning or decontamination procedure than those
recommended here, if you are not entirely sure that the intended procedure is safe for the
equipment.
Use only approved cleansers.
If in doubt, contact Thermo Fisher Scientific.

Cleaning

When cleaning centrifuge mind the following:

• Use warm water with a neutral solvent.

• Never use caustic cleaning agents such as soap suds, phosphoric acid, bleaching solutions or

scrubbing powder.

• Use a soft brush without metal bristles to remove stubborn residue.

• Afterwards rinse with distilled water.

• Use only disinfectants with a pH of 6-8.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.

Clean centrifuge and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Release the rotor.

5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

5-2 Sorvall RC-4 Thermo Scientific

6. Use a neutral cleaning agent with a pH value between 6 and 8 for cleaning.

Screws

CAUTION When cleaning, do not allow liquids, especially organic solvents, to get on the
drive shaft or the bearings of the centrifuge.
Organic solvents break down the grease in the motor bearing. The drive shaft could freeze
up.

After some applications their might be ice in the rotor chamber. Let the ice melt and drain it off. Clean
the rotor chamber as described above.

Cleaning the Venting Slots

For cleaning the venting slots proceed as follows:

1. Turn off the centrifuge.

2. Pull out the power supply plug.

3. Remove the lateral fastening screws and demount the venting grid.

5

Maintenance and Care

Cleaning the Venting Slots

Figure 5-1. Open centrifuge

4. Clean the cooling fins carefully with a brush. Make sure you do not deform the cooling fins.

5. Place the venting grid again and fastened the screws thoroughly.

Disinfection

Disinfect the centrifuge immediately whenever infectious material has spilled during centrifugation.

WARNING Infectious material can get into the centrifuge when a tube breaks or as
result of spills. Keep in mind the risk of infection when touching the rotor and take all
necessary precautions.
In case of contamination, make sure that others are not put at risk.
Decontaminate the affected parts immediately.
Take other precautions if need be.

Thermo Scientific Sorvall RC-4 5-3

a

5

Maintenance and Care

Decontamination

The rotor chamber should be treated preferably with a neutral disinfectant.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.
Observe the safety precautions and handling instructions for the cleaning agents used.

Contact the Service Department of Thermo Fisher Scientific for questions regarding the use of other
disinfectants.

Disinfect the rotor and accessories as follows:

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

4. Release the rotor.

5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

6. Treat the centrifuge according to the instructions for the disinfectant. Adhere strictly to the given
application times.

7. Wipe off the disinfectant.

8. Rinse thoroughly with water.

9. Dispose of the disinfectant according to the applicable guidelines.

Decontamination

Decontaminate the centrifuge immediately whenever contaminating material has spilled during
centrifugation.

WARNING Infectious material can get into the centrifuge when a tube breaks or as a
r
necessary precautions.
In case of contamination, make sure that others are not put at risk.
Decontaminate the affected parts immediately.
Take other precautions if need be.

CAUTION Before using any cleaning or decontamination methods except those
recommended by the manufacturer, users should check with the manufacturer that the
proposed method will not damage the equipment.

esult of spills. Keep in mind the risk of infection when touching the rotor and take all

For general radioactive decontamination use a solution of equal parts of 70 % ethanol, 10 % SDS
and water.

1. Open the centrifuge.

2. Turn off the centrifuge.

3. Pull out the power supply plug.

5-4 Sorvall RC-4 Thermo Scientific

4. Release the rotor.

5. Grasp the rotor with both hands and lift it vertically off the centrifuge spindle.

6. Remove the centrifuge tubes and adapters and dispose of them or disinfect them.

7. Rinse the centrifuge first with ethanol and then with de-ionized water.

• Adhere strictly to the given application times.

8. Wipe off the disinfectant.

9. Rinse thoroughly with water.

10. Dispose of the decontamination solution according to the applicable guidelines.

Service of Thermo Fisher Scientific

Thermo Fisher Scientific recommends having the centrifuge and accessories serviced once a year by an
authorized service technician. The service technician checks the following:

5

Maintenance and Care

Service of Thermo Fisher Scientific

• the electrical equipment;

• the suitability of the set-up site

• the lid lock and the safety system;

• the rotor;

• the fixation of the rotor and the drive shaft.

Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are
performed for free during the warranty period and afterwards for a charge.
This is only valid if the centrifuge has only been maintained by a Thermo Fisher Scientific service
technician.

;

Thermo Scientific Sorvall RC-4 5-5

6

Troubleshooting

Contents

• “Mechanical Emergency Door Release” on page 6-2

• “Troubleshooting by User” on page 6-3

• “When to contact a Service Technician” on page 6-5

Thermo Scientific Sorvall RC-4 6-1

6

Emergency Door Release

Troubleshooting

Mechanical Emergency Door Release

Mechanical Emergency Door Release

During a power failure, you will not be able to open the centrifuge door with the regular electric lid
release. A mechanical override is provided to allow sample recovery in the case of an emergency.
However, this should be used only in emergencies and after the rotor has come to a complete stop.

WARNING The rotor can still be spinning at high speed. If touched, it can cause serious
injuries

Always wait a few minutes until the rotor has come to a stop without braking. The brake does not work
when there is no current. The braking process lasts much longer than usual.

Proceed as follows:

1. Make sure the rotor has stopped (view port in the door).

WARNING Never use your hand or any tools to brake the rotor.

.

2. Pull

3. On both sides of the housing are two white plastic plugs which you can pry out of the back plate

out the power supply plug.

with a screwdriver or a knife.
Pull the release cords attached to it at the same time to trigger the mechanical door release.
The door will open and the samples can be removed.

Figure 6-1. Emergency Door Release

Note You need to pull both release cords in order to unlock both locks.

4. Push the release cords back into the centrifuge and mount the plug.

5. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the
key to have the door locks operative again.

6-2 Sorvall RC-4 Thermo Scientific

Troubleshooting by User

If problems occur other than those listed in this table, the authorized customer service
representative must be contacted.

Error Message Problem with Centrifuge Possible Causes and Cures

6

Troubleshooting

Troubleshooting by User

Display remains
dark.

Display fails briefly. The drive stops.

The centrifuge door
cannot be opened.

- Exceptionally running noise. Check the load placed in the rotor.

Message
appears in display.

bAl

The drive stops.

The run does not start or the centrifuge runs
down without being braked.

The centrifuge door cannot be opened.

Rotor stops with deceleration to standstill.

Message

Centrifuge does not open. Restart the centrifuge.

Rotor stops with deceleration to standstill. Imbalance detected.

E-14 appears in display.

No mains connection.

Check whether the switch is properly pressed.

Check the mains connection.

If the message appears again, inform a service technician.

Mains connection interrupted for some seconds.

Turn off mains switch.

Check whether the mains power cord is connected properly.

Restart the centrifuge.

If the message appears again, inform a service technician.

The emergency door release enables you to retrieve your samples.

If the message appears again, inform a service technician.

Check that the rotor cross bolts are well greased.

Restart the centrifuge.

If the message appears again, inform a service technician.

Check the load placed in the rotor.

Check that the bolts of the rotor are well greased.

Restart the centrifuge.

If the message appears again, inform a service technician.

The message
appears in display.

Thermo Scientific Sorvall RC-4 6-3

rotor

Rotor stops with deceleration. Set speed exceeds permissible maximum speed for the rotor. (The

same holds for RCF setting)

a. For approx. 15 sec. the maximum permissible rotor speed or rcf

for the inserted rotor will be shown alternately by
display.

Within this period, it is possible to accept this value by again
pressing the key. The centrifugation is then continued.

b. After 15 sec. the rotor will be decelerated. Always wait until

the rotor has come to a stop without braking.

By opening and closing the door you reset the message
After entering a permissible speed you can start again.

rotor in the

rotor.

6

Troubleshooting

Troubleshooting by User

Error Message Problem with Centrifuge Possible Causes and Cures

Display OPEN
appears although
door is closed.

Message
appears in display.

Message
appears in display.

Lid

Lift Lid

E-00

E-02

Centrifuge will not start. a. Centrifuge door not properly closed. Open the centrifuge door

and repeat locking procedure.

b. Over-temperature in the motor detected.

Pull out the power supply plug.

Control and clean the venting grid.

Wait some 20 minutes before starting the centrifuge again. If
the message appears again, inform a service technician.

The run does not start or the centrifuge runs
down without being braked.

Door does not open automatically. Door has not been lifted off the lock during opening.

Motor does not start. Motor or rotor are blocked.

The run does not start or the centrifuge runs
down without being braked.

Centrifuge door was opened manually during the run.

Close the door again. The centrifuge decelerates without braking.

Restart the centrifuge.

1. Avoid laying objects onto the centrifuge door.

2. Lift the door slightly.

Restart the centrifuge.

Open the centrifuge door.

Check whether the rotor can turn freely.

If the message appears again, inform a service technician.

Internal program error in the memory

Restart the centrifuge.

If the message appears again, inform a service technician.

E-03

E-04

E-06

E-07

E-08

E-10

The run does not start or the centrifuge runs
down without being braked.

The run does not start or the centrifuge runs
down without being braked.

The run does not start or the centrifuge runs
down without being braked.

The rotor is decelerated to a standstill. The
lid can be opened.

The run does not start or the centrifuge runs
down without being braked.

Self-test after switching on the centrifuge. NV-RAM; error in program memory

Error in speed entry.

Restart the centrifuge.

If the message appears again, inform a service technician.

Temperature measurement malfunction (sensor fracture).

Restart the centrifuge.

If the message appears again, inform a service technician.

Communication error between key panel and CPU.

Restart the centrifuge.

If the message appears again, inform a service technician.

Overheating in chamber.
Display > 51 °C or measured temperature > 70 °C.

Clean the cooling fins in the back. (Cooling machine might be

e.)

defectiv

Over voltage at frequency converter. Mains voltage outside the
tolerance. Deceleration resistance defective.

Restart the centrifuge.

If the message appears again, inform a service technician.

Restart the centrifuge.

If the message appears again, inform a service technician.

6-4 Sorvall RC-4 Thermo Scientific

Error Message Problem with Centrifuge Possible Causes and Cures

6

Troubleshooting

When to contact a Service Technician

E-12

E-14

E-15

E-17

E-19

E-22

E-24

The run does not start or the centrifuge runs
down without being braked.

Centrifuge does not start or decelerates to
standstill.

The run does not start or the centrifuge runs
down without being braked.

Door does not open. Door is blocked or jammed.

The centrifuge cannot be operated. Incorrect NV-RAM or key panel

The centrifuge cannot be operated. NV-RAM parameter does not fit the processor

The centrifuge cannot be operated. NV-RAM 2 missing

Temperature measurement error

Restart the centrifuge.

If the message appears again, inform a service technician.

No rotor present or rotor identification impossible.

a. Check whether a certified rotor is inserted.

b. Following a brief power failure, the rotor could not be

identified.

Restart the centrifuge.

If the message appears again, inform a service technician.

The check sum in the NV-RAM is incorrect.

Press the door down once at the front in the center and then
actuate the key again.

Otherwise see “Mechanical Emergency Door Release” on

page 6-2.

E-25

The run does not start or the centrifuge runs
down without being braked.

Start without rotor.

Open the centrifuge by pressing the key.

Check whether the rotor is installed and loaded properly.

Check whether a broken tube or damaged rotor released the
imbalance switch.

Restart the centrifuge.

If the message appears again, inform a service technician.

When to contact a Service Technician

If you need to contact a service technician, please provide the order no. and the serial no. of your
centrifuge. You will find these information at the top right.

To identify the software version, proceed as follows:

1. Turn on the power switch on the right side of the device.
The centrifuge performs a self-check of its software. Subsequently, the following readings will be
displayed. The order is:

Software version key panel

Software version

NV-RAM-Version 1

NV-RAM-Version 2

Cycle counter

Thermo Scientific Sorvall RC-4 6-5

6

Troubleshooting

When to contact a Service Technician

Note The values given are only examples.

2. Afterwards the different programs are tested as well.

3. Communicate the software version to the service technician.

Program test

6-6 Sorvall RC-4 Thermo Scientific

A

Chemical Compatibility Chart

CHEMICAL

2-mercaptoethanol

Acetaldehyde

Acetone

Acetonitrile

Alconox

Allyl Alcohol

Aluminum Chloride

Formic Acid (100 %)

Ammonium Acetate

Ammonium Carbonate

Ammonium Hydroxide (10 %)

Ammonium Hydroxide (28 %)

Ammonium Hydroxide (conc.)

Ammonium Phosphate

Ammonium Sulfate

Amyl Alcohol

Aniline

Sodium Hydroxide (<1 %)

Sodium Hydroxide (10 %)

Barium Salts

Benzene

Benzyl Alcohol

Boric Acid

Cesium Acetate

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

SSU- SMS- SUSSUSS- SSSSUSSSSSS

S-UU---M-U---MU U U MM- MS U - S - U

MSUUSUMSSUUSUSUUUS SUUSMMSUU

SSU- SMS- SSUSUMUU- SMUUSSSSUU

UUS- SSS- SSSSSSMSSSSSSSSSSSU

---U--S----S-SSMSSS- MS- - S- -

UUSSSSUSSSSMSSSS- SSSSSMUUSS

-SMU--U----U-SMUUSS-US-US-U

SSU- SSS- SSSSSSSU- SSSSSSSSSS

MSUSSSSSSSSSSSUU- SSSSSSMSSS

UUSUSSMSSSSS- SUMSSSSSSSSSMS

UUSUSUMSSSSSUSUMSSSSSSSSSMS

UUUUSUMS- S- SUSUUSSS- MSSSS- U

U- S- SSSSSSSS- SSM- SSSSSSMSSS

UMS- SSUSSSSSSSSS- SSSSSSUSSU

S- MU- - SS- M- S- MSSSSM---U-S-M

SSUUSUSMSUUUUUUU- SMUUSSSSUS

U- MSSS- - SMSS- SMMSSSSSSMSS- U

U- MU- - U- MMSSUSUUSSSSSSMSS- U

MUS- SSSSSSSSSSSM- SSSSSSMSSS

SSUUSUMUSUUSUUUMUMUUUSUUSUS

S- UU- - MM- M- SUUUUUUU- MSM- S- S

USSMSSUSSSSSSSSSUSSSSSSSSSS

M- S- SSS- SSSS- SS- - SSSSSSMSSS

, POLYCLEAR, CLEARCRIMP

1

NYLON

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

VITON

Thermo Scientific Sorvall RC-4 A-1

A

Chemical Compatibility Chart

CHEMICAL

Cesium Bromide

Cesium Chloride

Cesium Formate

Cesium Iodide

Cesium Sulfate

Chloroform

Chromic Acid (10 %)

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSSUSSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

UUUUSSMUSUUMUMUUUMMUUSUUUMS

U- UUSUU- SSSUSSMUMSSUMSMUSSS

VITON

Chromic Acid (50 %)

Cresol Mixture

Cyclohexane

Deoxycholate

Distilled Water

Dextran

Diethyl Ether

Diethyl Ketone

Diethylpyrocarbonate

Dimethylsulfoxide

Dioxane

Ferric Chloride

Acetic Acid (Glacial)

Acetic Acid (5 %)

Acetic Acid (60 %)

Ethyl Acetate

Ethyl Alcohol (50 %)

Ethyl Alcohol (95 %)

Ethylene Dichloride

Ethylene Glycol

Ethylene Oxide Vapor

Ficoll-Hypaque

Hydrofluoric Acid (10 %)

Hydrofluoric Acid (50 %)

U-UU-UU---SUUSMUMSSUMS-UM-S

SSU---S-SUUUUUU- - UU- USSSSUS

SSS- SSSUSUSSUUUMSMUMMSUMMUS

SSS- SSS- SSSSSSS- - SSSSSSSSSS

SSSSSSSSSSSSSSSSSSSSSSSSSSS

MSSSSSS- SSSSSSSSSSSSSSSMSSS

SSUUSSSUSUUSUUUUUUUUUSSSSMU

S- UU- - M- SU- S- MUUUMM- US- - SUU

SSU- SSS- SSUSUSU- - SSSMSSSSSS

SSUUSSS- SUSSUSUU- SSUUSSSSUU

MS U U S S MMS U U S U MU U - MMMU SSSSUU

UUS---MS-M-S-S---SS---MUS-S

SSUUSSUMSUSUUUUUMS UMUSUUS- U

SSMSSSMSSSSSMSSSSSSSMSSMSSM

S S U U S S U - S MS U U MU S MS MS MS MU S MU

MMUUSSMMSSUS UMUU- SSUUSMMSUU

SSSSSSMSSSSSUSUSSSSSSSSMSMU

SSSUSSMSSSSSUSU- SSSMSSSUSMU

S- UU- - SM- UUSUUUUUUU- USU- S- S

SSSSSSSSSSSS- SUSSSSSSSSMSMS

S- U- - U- - SU- S- SM- - SSSUSUSSSU

MSS- SSS- SSSS- SS- SSSSSSSMSSS

UUUM- - U- - UUS- SMUSSSSMSUUU- -

UUUU- - U- - UUUUSUUUSSMMSUUU- M

A-2 Sorvall RC-4 Thermo Scientific

CHEMICAL

Hydrochloric Acid (conc.)

Formaldehyde (40 %)

Glutaraldehyde

Glycerol

Guanidine Hydrochloride

Haemo-Sol

Hexane

A

Chemical Compatibility Chart

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

UUUU- UUM- UMUUMUUU- S- USUUU- -

MMMSSSSMSSSSMSSSUSSMSSSMSMU

SSSS- - S- SSSSSSS- - SSS- - SSS- -

MSS- SSSSSSSSSSSS- SSSSSSSSSS

UUS- SSS- SSSSSSS- - SSSSSSUSSS

SSS---S-SSSSSSS- - SSSSSSSSSS

SSS- SSS- SSUSUMUSSUSSMSUSSUS

VITON

Isobutyl Alcohol

Isopropyl Alcohol

Iodoacetic Acid

Potassium Bromide

Potassium Carbonate

Potassium Chloride

Potassium Hydroxide (5 %)

Potassium Hydroxide (conc.)

Potassium Permanganate

Calcium Chloride

Calcium Hypochlorite

Kerosene

Sodium Chloride (10 %)

Sodium Chloride (sat'd)

Carbon Tetrachloride

Aqua Regia

Solution 555 (20 %)

Magnesium Chloride

Mercaptoacetic Acid

Methyl Alcohol

Methylene Chloride

Methyl Ethyl Ketone

Metrizamide

Lactic Acid (100 %)

- - MU- - SS- U- SUSSMSSS- SSS- S- S

MMMU SSSSSUSSUSUMSSSSSSSMMMS

SSM- SSS- SMSSMSS- MSSSSSMSSMM

USS- SSS- SSSSSSSSSSS- SSSMSSS

MUSSSSS- SSSSSSUSSSSSSSSSSSS

USS- SSSSSSSSSSS- SSSSSSSUSSS

UUSSSSM- SSSS- SUSSSSSSSMUMSU

UUMU- - M- MSS- UMUUUSM- MU- UU- U

SSS- SSS- SSSUSSSM- SMSUSSMSUS

MUSSSSSSSSSSSSMS- SSSSSSMSSS

M- U- SMMS- M- S- SMS- SSSMSMUS- S

SSS- SSSUSMUSUMMS- MMMSSUSSUS

S- SSSSSS- - - SSSSS- SSSS- SSM- S

U-SUSSS----SSSSS- SS- S- SSM- S

U U MS S U MU S U U S U MU S S MMS MMMMU S S

U-UU--U-----UUUUUUU-----S-M

SSS---S-SSSSSSS- - SSS- SSSSSS

MSS- SSSSSSSSSSSSSSSSSSSMSSS

USU- SMS - SMSUUUU- SUUSMSUSSSS

SSSUSSMSSSSSUSUMSSSSSSSMSMU

UUUUMSSUSUUSUUUUUMUUUSSMUSU

SSUUSSMSSUUSUSUUUSSUUSSSSUU

MSS- SSS- SSSS- SS- - SSSSSSMSSS

--S------MSU-SSSMSS- MSMSS- S

Thermo Scientific Sorvall RC-4 A-3

A

Chemical Compatibility Chart

CHEMICAL

Lactic Acid (20 %)

N-Butyl Alcohol

N-Butyl Phthalate

N, N-Dimethylformamide

Sodium Borate

Sodium Bromide

Sodium Carbonate (2 %)

Sodium Dodecyl Sulfate

Sodium Hypochlorite (5 %)

Sodium Iodide

Sodium Nitrate

Sodium Sulfate

Sodium Sulfide

Sodium Sulfite

Nickel Salts

Oils (Petroleum)

Oils (Other)

Oleic Acid

Oxalic Acid

Perchloric Acid (10 %)

Perchloric Acid (70 %)

Phenol (5 %)

Phenol (50 %)

Phosphoric Acid (10 %)

Phosphoric Acid (conc.)

Physiologic Media (Serum, Urine)

Picric Acid

Pyridine (50 %)

Rubidium Bromide

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

--SS-----MSM-SSSSSSSMSMSS- S

S- SU- - S- - SM- USMSSSSMMSM- S- S

SSU- SSS- SUUSUUUM- UUSUSMMSUS

SSSUSMS- SSUSUSUU- SSUUSMSSSU

MSSSSSSSSSSUSSSS- SSSSSSMSSS

USS- SSS- SSSSSSSS- SSSSSSMSSS

MUSSSSSSSSSSSSUSSSSSSSSSSSS

SSS- SSS- SSSSSSS- SSSSSSSSSSS

UUMSSMUSSMSSSMSSSSMSSSMUSMS

MSS- SSS- SSSSSSS- - SSSSSSMSSS

SSS- SSSSSSSSSSSS- SSSSSUSSSS

USS- SSSSSSSSSSSSSSSSSSSMSSS

S-SS---S---SSSUU--S---SSM-S

SSS- SSSSMSSSSSSM- SSSSSSSSSS

USSSSS- SSS- - SSSS- SSSSSSMSSS

SSS---SUSSSSUUMSMUUSSSUSSSS

S-S---SMSSSSUSSSSUSSSS- SSMS

S- USSSUUSUSSMSSSSSSSSSMUSMM

UUMSSSUSSSSSUSUSSSSSSSSUMSS

U- U- SUU- SMM- - MUMSMM- MSU- S- S

UUU- - UU- SUMUUMUUUMMUMSUUSUS

U S U - S MM- S U MU U S U MS MS U U S U MMMS

USU- SUM- SUMUUUUUSUMUUSUUUMS

UUMSSSUSSSSU- SSSSSSSSSUMUSS

UUMM- - US- MSUUMMSSSMSMSUMU- S

MSSS- - S- SSSSSSSSSSSSSSSSSSS

SSU- SMSSSMSUSSSUSSSSUSUMSMS

USUUSUU- USSUUMUU- USMUSSUUUU

MSS- SSS- SSSSSSS- - SSSSSSMSSS

VITON

Rubidium Chloride

Sucrose

MSS- SSS- SSSSSSS- - SSSSSSMSSS

MSS- SSSSSSSSSSSSSSSSSSSSSSS

A-4 Sorvall RC-4 Thermo Scientific

CHEMICAL

Sucrose, Alkaline

Sulfosalicylic Acid

Nitric Acid (10 %)

Nitric Acid (50 %)

Nitric Acid (95 %)

Hydrochloric Acid (10 %)

Hydrochloric Acid (50 %)

Sulfuric Acid (10 %)

Sulfuric Acid (50 %)

Sulfuric Acid (conc.)

Stearic Acid

Tetrahydrofuran

Toluene

Trichloroacetic Acid

Trichloroethane

Trichloroethylene

Trisodium Phosphate

Tris Buffer (neutral pH)

Triton X-100

Urea

Hydrogen Peroxide (10 %)

Hydrogen Peroxide (3 %)

Xylene

Zinc Chloride

Zinc Sulfate

Citric Acid (10 %)

1

Polyethyleneterephthalate

A

Chemical Compatibility Chart

, POLYCLEAR, CLEARCRIMP

MATERIAL

ALUMINUM

ANODIC COATING for ALUMINUM

BUNA N

CELLULOSE ACETATE BUTYRATE

POLYURETHANE ROTOR PAINT

COMPOSITE Carbon Fiber/Epoxy

DELRIN

ETHYLENE PROPYLENE

GLASS

NEOPRENE

NORYL

NYLON

1

PET

POLYALLOMER

POLYCARBONATE

POLYESTER, GLASS THERMOSET

POLYTHERMIDE

POLYRTHYLENE

POLYPROPYLENE

POLYSULFONE

POLYVINYL CHLORIDE

RULON A, TEFLON

SILICONE RUBBER

STAINLESS STEEL

TITANIUM

TYGON

MSS- SSS- SSSSSSUSSSSSSSSMSSS

UUSSSSS- SSSUSSS- SSS- SSSUSSS

USUSSUU- SUSU- SSSSSSSSSMSSSS

USUMSUU- SUSUUMMUMMMSSSUSSMS

U- UU- UU- - UUUUMUUUUMUUSUSS- S

UUMSSSU- SSSUUSUSSSSSSSSUMSS

UUUUSUU- SMS UUMUUSSSSMSMUUMM

MUUSSUU- SSMUSSSSSSSSSSUUUSS

MUUUSUU- SSMUUSUUMSSSSSUUUMS

MUUU- UUM- - MUUSUUUMSUMSUUU- S

S-S---SMSSSS- SSSSSSSSSMMSSS

SSUUSUUMSUUSUUU- MUUUUSUSSUU

SSUUSSMUSUUSUUUSUMUUUSUSUUM

UUU- SSUMSUSUUSM- MSS UUSUUUMU

S-U---MU-U-SUUUUUUUUUSU- S- S

--UU---U-U-SUUUUUUUUUSU- U- S

---S--M------S--SSS--S--S-S

USSSSSS- SSSSSSSSSSSSSSSSSSS

SSS- SSS- SSSSSSSSSSSSSSSSSSS

S- USSSS----SSSMSSSS- SSSMS- S

UUMSSUU- SSSUSSSMUSSSSSSMSUS

SMSSS- S- SSSSSSSSMSSSSSSSSSS

SSUSSSMUSUUUUUUMUMUUUSUMSUS

UUSSSSUSSSSSSSSSSSSSSSSUSSS

USS- SSSSSSSSSSSSSSSSSSSSSSS

MSSMSSMSSSSSSSSSMSSSSSSSSSS

VITON

Thermo Scientific Sorvall RC-4 A-5

A

Chemical Compatibility Chart

Key

S Satisfactory

M Moderate attack, may be satisfactory for use in centrifuge depending on length of exposure, speed involved, etc.

U Unsatisfactory, not recommended.

-- Performance unknown; suggest testing, using sample to avoid loss of valuable material.

Chemical resistance data is included only as a guide to product use. No organized chemical
resistance data exists for materials under the stress of centrifugation. When in doubt, we recommend
pretesting sample lots.

Suggest testing under actual conditions of use.

A-6 Sorvall RC-4 Thermo Scientific

I

Index

A

Acceleration Curve ..........................................4-4

Accident Prevention .......................................... iv

Adjust Voltage and Frequency ......................... 2-6

Aerosol-tight Rotors ......................................4-10

Aligning the Centrifuge ...................................2-4

Audible Alarm ...............................................4-10

B

Before Setting up .............................................2-2

Before Use ....................................................... 2-1

Braking Curve ................................................. 4-4

Bucket Selection .............................................. 4-7

C

Care ................................................................ 5-1

Centrifugation ................................................. 4-8

Centrifuge Door ..............................................4-2

Changing the Settings during a Run ................4-8

Characteristics ................................................. 1-2

Chemical Compatibility Chart ....................... A-1

Cleaning ..........................................................5-2

Cleaning Intervals ...........................................5-2

Cleaning the Venting Slots ..............................5-3

Close Door ...................................................... 4-2

Continuous Operation ....................................4-6

Control Panel ...........................................3-1, 3-2

Error Message ..................................................6-3

Explanation of RCF Value ..............................

Explanation ofK-Factor ...................................4-5

4-5

F

Features ...........................................................1-4

Functions ........................................................ 1-4

G

Guidelines .......................................................1-4

I

Imbalance Indicator ........................................4-8

Intended Purpose .............................................. iv

K

Key Switch .................................................... 4-11

Keys ................................................................3-2

L

Limited Time Mode ........................................ 4-6

Location ..........................................................2-4

M

Mains Connection ...........................................2-5

Mains Supply ..................................................1-5

Maintenance ................................................... 5-1

D

Decontamination ............................................ 5-4

Directives ........................................................1-4

Disinfection ....................................................5-3

Door Opening ................................................ 4-2

E

Emergency Door Release .................................6-2

Entering Parameters ........................................ 4-4

Thermo Scientific Sorvall RC-4 I-1

O

Operation ........................................................ 4-1

P

Precautions ........................................................ iv

Pre-cooling the Centrifuge ..............................4-7

Preface ............................................................... iii

Preselecting Speed / RCF ................................4-4

Preselecting the Temperature ..........................4-6

Index:

Pre-warming the Centrifuge ............................4-7

R

Removing the Rotor ........................................4-9

Rotor Installation ............................................4-3

Rotor Selection ................................................1-6

Run Time Preselection .................................... 4-5

S

Scope of Supply ................................................. iii

Service ......................................................5-5, 6-5

Setting up ........................................................2-2

Setting up a Program ....................................... 4-7

Short-term Centrifugation .............................. 4-9

Standards ........................................................1-4

Starting Centrifuge Program ...........................4-8

Stopping the Centrifugation Program .............4-9

Storage ............................................................ 2-6

Switch on Centrifuge ......................................4-2

T

Technical Data ................................................ 1-3

Temperature Adaptation during Standstill ......4-9

Transporting the Centrifuge ............................ 2-4

Troubleshooting .............................................. 6-1

Troubleshooting by User ................................. 6-3

Turning off the Centrifuge ............................4-10

I-2 Sorvall RC-4 Thermo Scientific

Thermo Electron LED GmbH

Zweigniederlassung Osterode
Am Kalkberg, 37520 Osterode am Harz
Germany

thermofisher.com/centrifuge

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20057791 is the original instr uction manual.

en

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All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated.

Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark
of Hongye CO., Ltd. Hypaque is a registered trademark of Amersham Health As. RULON A and Tygon are registered trademarks of Saint-Gobain
Performance Plastics. Alconox is a registered trademark of Alconox. Ficoll is a registered trademark of GE Healthcare. Haemo-Sol is a registered
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representative for details.
Shown pictures within the manual are examples and may differ considering the set parameters and language. Pictures of the user interface within
the manual are showing the English version as example.