Thermo Scientific Phadia 100 User Manual

Phadia® 100

English

12-3501-20/12

User Manual

This User Manual is intended to be a convenient source of product informa­tion for the user of the Phadia100 instrument. Further information is found in
the Directions for Use for the various reagent products and in the manuals for
the optional software.

Information in this document is subject to change without notice.
All pictures displayed in this manual should be regarded as examples only.
This User Manual is not country specific and therefore reference may be

made to methods and/or assays that are not registered or cleared for use in a
specific country. Please consult your local sales office for country specific
information regarding the regulatory status of particular assays or methods
on Phadia 100.

The following designations are trademarks belonging to Thermo Fisher
Scientific Inc and its subsidiaries:
Elia, ImmunoCAP, Phadia, Phadiatop, Quality Club, UniCAP.

Trade mark change

Phadia AB has changed the trademarks of the instrument platforms from
"UniCAP

®

" and "ImmunoCAP®" to "Phadia®". The new name has been
applied to the instruments and related items, e.g. Software and User Manu­als. The trademark "ImmunoCAP

®

" has been removed from the System
Reagents. This is a trademark change only; the change has no impact on
performance or safety.

Embedded third party software: The Phadia 100 instrument may contain
third party software, e.g. third party software from Microsoft. The third
party software is subject to end user license terms of the respective software,
which the user must accept before any use of the Phadia 100 instrument. The
third party software is provided only for use in accordance with these end
user license terms. Any other use of the third party software is strictly
prohibited. Furthermore, the user may not install any additional software to
the Phadia 100 instrument without the prior written approval of Phadia AB.

Phadia 100 User Manual, version 12. This version is valid for Phadia 100

Instrument Version 1.0 and Phadia 100 Instrument Software Version 3.0.

© 2012 Thermo Fisher Scientific Inc and its subsidiaries. All rights reserved.

Manufacturer: Phadia AB,

Rapsgatan 7P, P.O. Box 6460, 751 37 Uppsala, Sweden.

Issued October 1995. Revised May 2012.

ArtNo. 12-3501-20/12

1. Instrument Specification and Installation

2. Routine Assay Run

3. Reagent and Sample Handling

4. Assay Run Editing Functions

5. Assay Run Data Evaluation

6. Assay Run Quality Control

7. Monthly Calibration

8. Quality Assurance

9. Methods, Test Panels, Article Management

10. Printouts

11. Maintenance

12. Error Handling

13. Repacking

14. Accessories

15. Index

1

2

3

4

5

6

7

8

9

10

11

13

14

15

16

12

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1

Chapter 1 - Instrument Specification and

Installation

This chapter describes technical

specifications and installation procedure

Instrument 2

Intended Use 2

Principal functionality 2

Instrument Guide 3

Sample Carousel 5

Instrument Lifting Points 5

ImmunoCAP/EliA Well Dispenser 6

USB ports/Diskette Drive 6

TCP/IP connector 6

Washing Solution, Rinse Solution and Waste Bottles 7

ImmunoCAP Carousel 7

ImmunoCAP Waste Container 8

Technical Specification 9

Instrument Specification 9

Solid Phase Specification 13

Operational Precautions and Limitations 14

Installation 16

Contents 17

Installation Instructions 1 8

Parameter Set Up 22

Date/Time 22

Language 23

Default Method 23

Set sIgE Method 2 4

Contrast 24

Set and Change Passwords 2 5

Communication Parameters 2 6

Configure Phadia 100 for TCP/IP 27

Configure computer name 2 8

Instrument Specification and Installation Phadia 100 - User Manual

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Instrument

Intended Use

Phadia 100

Phadia 100 is a fully automated instrument including software to be used
together with dedicated in vitro diagnostic tests. The instrument is designed to
handle processing of samples, reagents and measuring results and it can be
connected to the software Phadia Information Data Manager (IDM). Phadia 100
is intended to be used in clinical laboratories.

Principal functionality

Phadia 100 is designed to be used together with ImmunoCAP and EliA in vitro
diagnostic tests manufactured by Phadia AB. Phadia 100 yields the same
analytical results from samples of the same specimen as Phadia 250, Phadia
1000, Phadia 2500 and Phadia 5000 within stated tolerance limits. The
instrument includes functions for-distribution of samples, ImmunoCAP and EliA
wells, and reagents,-processing of all assay steps for incubation and washing,­reading of measuring values, calculations of analytical results, calculation of
statistics and result reporting.The algorithms included in the instrument
software used for calculation of analytical results and statistics are the same as
the ones included in Phadia Information Data Manager (IDM).Up to five
instruments can be connected to an external computer and communicate with
IDM software. When the instrument is connected to IDM software the measuring
values are electronically transferred and calculations of analytical results,
calculation of statistics and result reporting is done by IDM software. Phadia
100 is designed to handle approximately 100 tests per working day.

Solid Phase

For the measurement of the antibodies Phadia 100 can process ImmunoCAP or
EliA Wells. All procedures for EliA Wells should be performed as described for
ImmunoCAP.

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Instrument Guide

Instrument Front

ImmunoCAP/
EliA Well Dispenser

Printer

Processing Lid/
Processing Chamber

Pipette Arm

Washing Solution,
Rinse Solution and
Waste Bottles

USB ports/
Diskette Drive

Keyboard

Sample Carousel
Cover

Sample
Carousel

Pipette

Display

ImmunoCAP/EliA Well
Waste Container

Processing Lid open

Processing Chamber

ImmunoCAP Carousel

Drainage filter

Wash Rake

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Instrument Rear

Instrument equipped with USB ports:

Instrument equipped with diskette drive:

COM 2 port
(COM 1 is used
internally in
the instrument)

Mains Power Inlet

Washing Solution,
Rinse Solution and
Waste Bottles

Cooling fan

Voltage selector
and Primary fuses

Power on/off

Secondary fuses

VGA connector
(for service only)

TCP/IP connector

USB ports

COM 3 COM 2 COM 1

Mains Power Inlet

Washing Solution,
Rinse Solution and
Waste Bottles

Cooling fan

Voltage selector
and Primary fuses

Power on/off

Secondary fuses

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1

Instrument Lifting Points

Lift the instrument by holding under the instrument. Do not lift the instrument
by the areas indicated in the picture. Too much weight on these spots might
damage the instrument.

Sample Carousel

In the sample carousel you place the reagents
and samples in given positions. The positions
are:

1-48 Positions for calibrators, curve

controls, qualitycontrols, samples,

diluent and dilution tubes
A- D Positions for Conjugate
E Position for Development Solution
F Position for FluoroC
G Position for Diluent
H Position for Stop Solution

Please lift here!

Do not lift here!

Please lift here!

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ImmunoCAP/EliA Well
Dispenser

You load ImmunoCAP/EliA Well carriers in the
ImmunoCAP/EliA Well Dispenser. The
ImmunoCAP/EliA Well Dispenser is placed on
top of the processing chamber. Push the cap
away and push the carrier downwards until you
feel it clicking into place.

You will be prompted which ImmunoCAP/EliA
Well carrier to load when you are in the Load
and Start mode. The instrument will
automatically place ImmunoCAP/EliA Well in
the right position in ImmunoCAP Carousel.

USB ports/Diskette Drive

On instruments equipped with USB ports, you
find two USB 2.0 type A ports to the upper right
of the instrument and two USB 2.0 type A ports
on the instrument rear. You can store data on a
USB memory or an external USB hard drive,
and use it in a computer. You can also import
data from a USB memory or an external USB
hard drive, to be used in the instrument. To
connect a barcode reader of USB type, please
contact your local Phadia representative.

On instruments equipped with a diskette drive,
you find the diskette drive to the upper right of
the instrument. You can store data on a diskette
and use it in a computer. You can also import
data from a diskette to be used in the instru­ment.

TCP/IP connector

On instruments equipped with USB ports, you
find a TCP/IP connector to the upper right of the
instrument rear. The TCP/IP connector can be
used for communication to an external com­puter using the software IDM (Phadia Informa­tion Data Manager).

TCP/IP connector

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1

ImmunoCAP
Holder Disc

Elution Wells
Disc

NOTE! Do not unscrew the cap from the bottles
if the tubings are still connected. This can cause
problems with insufficient distribution of Wash­ing and Rinse Solution due to twisted and stuck
tubings inside the instrument.

Washing Solution, Rinse Solution and
Waste Bottles

You find the Washing Solution, Rinse Solution
and waste bottles to the left of the instrument,
behind the Sample Carousel. When you connect
these bottles to the instrument, please be sure you
connect them in the correct order. The bottles
and tubings from the instrument are marked with
different colours to indicate the correct connec­tion.

There is a quick-release function on the top of the
nozzles. Press the little button on the nozzle and
the tubing will come off immediately. When you
put the tubing back on the bottle, press the nozzle
on until you hear a clicking sound.

Fill the bottles with 1l of Washing Solution and 1l Rinse Solution for one assay
run. When running ImmunoCAP Specific IgA, ImmunoCAP Specific IgG or
ImmunoCAP Specific IgG4 with a full sample carousel, 1 l of Washing Solution
is not enough. Fill up the bottle with Washing Solution.

We recommend that you prepare Washing Solution for a week's consumption
and use this to fill the instrument.

ImmunoCAP Carousel

ImmunoCAP Carousel is placed in the processing chamber. You
find the processing chamber in the middle section of the
instrument. The chamber is opened by pressing the lid open
key. To open the lid, the power must be on. The lid cannot be
opened during processing. ImmunoCAP Carousel consists of
two units, the Elution Wells Disc and ImmunoCAP Holder Disc.

NOTE! ImmunoCAP Holder Disc is fragile and should be
handled as glass. Always replace if dropped on floor.

WARNING! Washing Solution may cause
sensitization by skin contact. Wear protective
gloves!

Instrument Specification and Installation Phadia 100 - User Manual

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ImmunoCAP Waste Container

ImmunoCAP Waste Container is located on the lower left side of
ImmunoCAP Carousel in the Processing Chamber. The instru­ment will not indicate when the container is full.

NOTE! ImmunoCAP Waste Container has to be emptied after
every assay run.

WARNING! Waste bottle, ImmunoCAP waste
container and process chamber may be
contaminated by potentially infectious,
biohazard material - take appropriate actions.
Wear protective gloves!

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Technical Specification

Instrument Specification

Dimensions Width: 680 mm

Depth: 600 mm
Height: 440 mm

Weight Instrument: 46 kg

Total Weight (packaged): 86 kg

Mains Power Supply AC 100 V, 50/60 Hz

AC 120 V, 50/60 Hz
AC 230 V, 50/60 Hz
AC 240 V, 50/60 Hz
Main voltage tolerances +10% / -15%
Main frequency tolerances ±1%
Designed for continuous operation
NOTE! ImmunoCAP 100 must be connected
to a grounded power outlet (protective
ground).

Power 550 VA
Primary Fuses 100/120 V T 5 A L

230/240 V T 2.5 A L
NOTE! At time of delivery no primary fuses
are inserted. The main voltage selector is set
to 230 V.

Secondary Fuses F903 T 1 A L

F904 T 100 mA L
F905 T 63 mA L
F906 T 6.3 A L
F907 T 2.5 A L
F908 T 5 A L
F909 T 63 mA L

Capacity 48 samples loaded at the same time and

processed in each batch

Features Automated dispensing

Automated processing
Automated measuring

Instrument Specification and Installation Phadia 100 - User Manual

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Working Conditions Ambient temperature: 18ºC to 32ºC

Ambient air humidity: 10% to 85% RH
Ambient air pressure: 860 hPa to 1060 hPa
Ambient temperature change: 2ºC/hour
(max.)

Heat Contribution 310 W (average) 1058 Btu/h

Storage Conditions Ambient temperature: -20ºC to +70ºC

Ambient air humidity: 10% to 95% RH

Ambient Sound Level 57 dBA
Warm-Up Time For normal assay: 1 hour
Interface ImmunoCAP 100 equipped with USB ports:

Two USB 2.0 type A ports are located at the
upper right of the instrument and two USB 2.0
type A ports are located at the instrument rear.
The USB ports can be used to connect USB
memorys or external USB harddrives.

One TCP/IP connector is located at the upper
right of the instrument rear. The TCP/IP
connector can be used for connection to IDM
(Phadia Information Data Manager).

One serial port (COM 2) is located at the upper
left of the instrument rear. (COM 1 is used
internally in the instrument.) This connection
will handle serial data communication in
accordance with the RS 232 C standard. The
COM 2 port can be used to connect a bar code
reader.
Pin characteristics (pin no., name, output/ input,
maximum voltage).
Pin 3 Tx output ±12 V
Pin 2 R x input ±12 V
Pin 7 RTS output ±12 V
Pin 8 CTS input ±12 V
Pin 6 DSR input ±12 V
Pin 4 DT R output ±12 V
Pin 5 GND signal ground
Pin 1 DC D input ±12 V
Pin 9 R I input ±12 V
The serial port must be connected only to
external equipment meeting the appropriate
EMC and electrical safety standards
(i.e., IEC 1010-1, IEC 601-1, IEC 950,
EN 61010-1, EN 60601-1, EN 60950 or
equivalent UL-standard)

Phadia 100 - User Manual Instrument Specification and Installation

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Volume

ImmunoCAP 100 equipped with diskette drive:

The serial ports (COM 1, COM 2 and
COM 3) are located at the upper part of the rear
side of the instrument. These connections will
handle serial data communication in accordance
with the RS 232 C standard.
COM 1, COM 2 and COM3 ports can be setup
for three different kinds of usage.

1) Serial communication to an external
computer

2) Serial communication to chain
communication

3) Barcode reader

Pin characteristics (pin no., name, output/ input,
maximum voltage).
Pin 3 Tx output ±12 V
Pin 2 Rx input ±12 V
Pin 7 RTS output ±12 V
Pin 8 CTS input ±12 V
Pin 6 DSR input ±12 V
Pin 4 DTR output ±12 V
Pin 5 GND signal ground
Pin 1 DC D input ±12 V
Pin 9 R I input ±12 V

These serial ports must be connected only to
external equipment meeting the appropriate EMC
and electrical safety standards
(i.e., IEC 1010-1, IEC 601-1, IEC 950,
EN 61010-1, EN 60601-1, EN 60950 or
equivalent UL-standard)

Liquid Containers Washing Solution bottle volume: 1 litre

Rinse Solution bottle volume: 1 litre
Waste bottle: 2 litres

Rinse Solution Purified water. For references, see

Directions for Use for Washing Solution
(10-9422-01, 10-9202-01).

Washing Solution Purified water, Washing Solution Concentrate,

Washing Solution Additive

Accuracy Pipette 40 µl: 50 µl: 90 µl: 200 µl:

±0.5 µl ±0.5 µl ±0.9µl ±2 µl (me an va l ue)

Washing Solution 137.5 µl ±7.5 µl (mean value)
Accuracy

Instrument Specification and Installation Phadia 100 - User Manual

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Electrical Safety Phadia 100 complies with the following

directive and standards:

- Low Voltage Directive 73/23/EEC

- In Vitro Diagnostics Directive 98/79/EC

Electrical Safety UniCAP

®

100

Standards used:
EN 60601-1: 1990, amendments A1, A11
and A12: 1993, pr A2 and pr A13: 1995
(IEC 601-1, second edition, 1988,
amendment 1, 1991, and amendment 2,

1995) and EN 61010-1: 1993 and pr A2:
1995 (IEC 1010-1, first edition, 1990,
amendment 1, 1992, and amendment 2,

1995)

Phadia 100/ImmunoCAP 100E

Standards used:
IEC 61010-1:2001 (EN 61010-1:2001)
IEC 61010-2-081:2001 and A 1
(EN 61010-2-081:2002)
IEC 61010-2-101:2002
(EN 61010-2-101:2002)

Electro Magnetic UniCAP

®

100

EN 61000-6-3 (2006) Generic emmission
standard for residential, commercial and
light industrial environments.
EN 61000-6-2 (2006) Generic immunity
standard for industrial environments.
EN 61326 (1997) with amendment 61326/A1
and 61326/A2

Phadia 100/ImmunoCAP 100E

EN 61326:2004 Generic emmission standard
for residential, commercial and light
industrial environments.
EN 61326:2004 Generic immunity standard
for industrial environments.

Service It is very important to maintain Phadia100

regularly as described in chapter 11,
Maintenance. Negligence of the maintenance
leads to Processing Errors as well as Assay
Result Errors. Problems can occur which have
to be solved by an authorized Phadia AB service
technician. Before handing over Phadia 100 for
service, it must be cleaned with Maintenance
Solution kit. Please follow the Directions for
Use in Maintenance Solution kit.

Classification

Compatibility

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Training All necessary information about installation

and how to operate Phadia 100 safely is included
in the shipment. However, if you feel that
additional training is required please contact
your local Phadia AB representative.

Solid Phase Specification

ImmunoCAP

ImmunoCAP are flexible hydrophilic polymer carriers encased
in a capsule. The carrier consists of an activated cellulose
derivative.

Allergen ImmunoCAP carriers, each containing 16 or 10
ImmunoCAP, or packaged as 6 carriers, are separately avail­able to permit individual selection of the most suitable allergen
panel.

When handling ImmunoCAP, keep the carrier closed to avoid
evaporation of buffer. Do not leave the carrier open for more
than 1 day at room temperature. Otherwise, discard the first
ImmunoCAP.

EliA Well

EliA Wells are Polystyrene Wells coated with antigens or
antibodies.

The wells are packed in carriers, which are additionally stored
in sealed aluminum foilbags with desiccant.

After dispensing EliA Wells, always store the carriers in the
foilbags, which must be properly resealed for stability reasons.
The indicator on the desiccant in the foilbag should always have
a blue color.

NOTE! For stability reasons it is important to
store ImmunoCAP and EliA Wells at 2° C to 8° C.
EliA Wells are moisture sensitive and must be
stored with desiccant.

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Operational Precautions and Limitations

This warning label is located below the Pipette
Arm. There is a risk of skin damage because the
pipette, which carries human body liquids, may
move without warning. Please, keep your hands
out of pipette range.

This warning label is to be placed to the left of
and on the right hand side of the sample carousel
compartment (see Installation Instructions).
When handling human blood, be aware that
there is a biological risk involved.

This warning label is to be placed on the waste
bottle (see Installation Instructions). Phadia 100
waste bottle collects waste liquids containing
human body fluids, which might be infected.
When emptying and cleaning the waste bottle,
take precautions to avoid direct contact with the
liquids. Wear protective gloves.

To ensure the minimizing of disposal of Waste
from Electrical and Electronic Equipment
(WEEE), this label is placed on the Rear Panel
of the instrument, according to the standard for
marking of electrical and electronic equipment
in accordance with Article 11(2) of Directive
2002/96/EC (WEEE), EN 50419. (This applies
within EU countries, Switzerland and Norway
since August 2005.) For disposal of the
instrument, please contact your local Phadia
representative.

This label is a contamination restraint marking
only applicable in the People's Republic of
China. It is placed on the Rear Panel of the
instrument.

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This label guarantees that Phadia 100 complies
with electrical safety standards used in the USA
and Canada. It is placed on the Rear Panel of the
instrument.

Limitations of Up to four conjugates can be placed in the

Sample Carousel. This means that the maximum
number of methods that can be run at the same
time is four. EliA methods can not be used
together with ImmunoCAP methods.

Precaution Phadia 100 is intended for in vitro diagnostic

testing, using reagents of Phadia only. When
operating Phadia 100, reagents manufactured
from human blood components are frequently
used. The source materials have been tested by
immunoassay for hepatitis B surface antigen
and for antibodies to HIV 1, HIV 2 and hepatitis
C and found to be negative. Nevertheless, all
recommended precautions for handling of blood
derivatives should be observed. Please refer to
HHS publication No. (CDC) 93-8395 or local/
national guidelines on laboratory safety
procedures.

the Procedure

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Installation

Accessory box

Spacing collars

Manual box

Save the empty box!
This box is designed and manufactured for this

instrument only and should be used in future
transportation. Save the metallic clips, the
screws and the spacers!

NOTE! Do not unpack until the instrument has reached room temperature. If
major temperature differences exist (~10ºC), condensation will occur in the
instrument.

1. Remove the metallic clips

at the top of the plywood box
with a screwdriver. Save the
metallic clips.

2. Lift the cover of the ply-

wood box and place it beside
the instrument. If wanted lift
the plywood box straight from
the wooden bottom plate and
place it beside the instrument.

3. Remove the four spacing

collars that are placed around
the instrument.

4. For further information see

the Installation Instructions.

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Contents

Phadia 100 is delivered in one box containing the instrument and the accessory
box. The contents in the accessory box should be according to the following list.

Accessory Box

The following accessories are included: Quantity
Mains kit 100/120V 1
Mains kit 230/240V 1
Printer paper (two rolls) 1
ImmunoCAP Holder Disc 1
Elution Wells Disc 1
Sample Carousel 1
ImmunoCAP Waste Container 1
Labels kit 1
Fuse kit 1
Plastic bag 1
Allen key 1
Wrench 1
Feet 4
Media 1

containing
Phadia 100 User Manual
A short introduction fo Phadia 100
How to run an assay in Phadia 100

Instrument Specification and Installation Phadia 100 - User Manual

Page 1.18 12-3501-20/12

Installation Instructions

1. Remove the accessory and the manual

box.
Choose a place with stable ambient tem-

perature for the instrument. Do not place it
in sunlight, draft or direct air stream from
air conditioning. Ensure that there is ap­proximately 30 cm space around all sides of
the instrument.

2. Lift the instrument by the carrying

handles on the wooden bottom plate and
place it on a table.

NOTE! This step requires two persons.

3. Let the instrument rest

on its back on a table with
the wooden bottom plate
hanging outside the table.
For doing this, lift the in­strument as instructed in the
part Instrument Lifting
Points. Remove the wooden

Screw the four feet
onto the instrument

Hexagonal screws
(outside the wooden
bottom plate)

Wooden bottom plate
Spacers (between the

wooden bottom plate
and the instrument)

bottom plate using the Allen
key to unscrew the hexago­nal screws. Then unscrew
the spacers with the 30 mm
wrench. Save the screws and
the spacers in the added plas­tic bag.

Table

4. Take the feet and screw them evenly onto the instrument. Do not screw

the feet too tightly (leave about 8-12 mm of threads visible).

5. Place the instrument on

a smooth even area. A stable
table is recommended. Level
the instrument using the feet,
but do not tighten the feet
too much. The instrument
should be properly levelled a
few millimeters lower on the
side where the Waste Bottle
is placed.

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NOTE! Phadia 100 must
only be connected to a
grounded power outlet
(protective ground).

8. Take out the Sample

Carousel from the acces­sory box. Place it on the
left side in front of the
bottles. The vial holders
and the sample holders
are in their positions.

9. Choose the proper

mains kit to comply with
your mains voltage. You
find the mains kit and the
fuse kit in the accessory box.

10. Select the correct mains kit.

Discard the unwanted kit imme­diately. Remove the warning la­bel covering the fuse/voltage se­lector unit on the rear panel of
the instrument.
Open the fuse/voltage selector
cover using a screwdriver. Insert
the screwdriver at the top centre
and apply pressure.

11. Turn the power on in order to open the processing chamber. The chamber

is opened by pressing the lid open key.

Place the enclosed fuses in the
fuse holders and install the fuse
holders in both the left and right
hand position.

Remove the voltage selector
switch and select the correct
mains voltage by turning the
voltage selector. Place the
switch in the instrument. Close
the cover and make sure that
the selected voltage is visible
through the window. Connect
the main supply using the en­closed cord.

6. Remove the tape from

the Pipette Arm. Gently lift
the arm straight up and move
it to the right, away from the
Sample Carousel.

7. Remove the tape from the Sample Carousel Cover and the tape

around the Rinse Solution, Washing Solution and Waste bottles.

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Cut the end of the paper to
an arrow. Insert the paper
in the printer. Paper should
be directed from the rear to
the front.

ImmunoCAP Holder Disc

Guide posts

NOTE! The ImmunoCAP
Holder Disc is fragile and
should be handled as glass.
Replace if dropped on floor.

NOTE! Before closing the pro­cessing lid, check that the
Wash Rake is not in its top or
bottom position, otherwise
it might break something in­side. The Wash Rake can be
moved manually and should
be set in a middle position.

Elution Wells Disc

Do not exchange Elution Wells Disc between
instruments. Write the serial number of the
instrument on the Elution Wells Disc.

Roll Pin

Paper Roll

12. Take out Elution Wells

Disc and ImmunoCAP Holder
Disc from the accessory box
and place them in the Process­ing Chamber. Place the
ImmunoCAP Holder Disc on
top of the Elution Wells Disc
in the Processing Chamber.
Align the guide posts.

Combined together they are
called ImmunoCAP Carousel.

13. Take out the Immuno-

CAP Waste Container and
place it in the left corner at the
front of the Processing Cham­ber. Make sure that the
ImmunoCAP Waste Con­tainer is hanging loosely un­der the instrument.

14. Load the paper roll as described in chapter 11, Main-

tenance.

15. Fill the Rinse Solution

Bottle with purified water and
the Washing Solution Bottle
with the appropriate Wash­ing Solution.

For preparation of the Wash­ing Solution, please read the
Directions for Use in the
ImmunoCAP Washing Solu­tion kit.

16. Connect the tubings with

the correct bottles. See colours
on bottles and tubings for guid­ance.

Place the two multilingual bio­hazard labels on each side of
the waste bottle.

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18. Place warning labels with appropriate

language to the left of and on the right hand
side of the sample carousel compartment.

19. The instrument should

now be ready to use.

17. A warning label in english

is placed below the Pipette
Arm. If required place a warn­ing label with appropriate lan­guage on top of the english
label.

WARNING! Do not use radio transmitters or
mobile telephones within a radius of 2 metres
from Phadia 100. The use of such devices close to
the instrument may impair the instrument's
performance.

Instrument Specification and Installation Phadia 100 - User Manual

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Parameter Set Up

It is possible to change the default settings for different parameters to adapt
Phadia 100 to your needs.

Date/Time

1. In Prepare Run mode press

6.

2. Press yes.

3. In Install/Service mode

press 3.

4. In Date/Time mode press

yes.

5. If the displayed year is

correct press enter. If you wish
to change year type the year
you want and press enter.

6. If the displayed month is

correct press enter. If you wish
to change month type the
month you want and press
enter.

7. If the displayed day is

correct press enter. If you wish
to change day type the day you
want and press enter.

8. If the displayed hour is

correct press enter. If you wish
to change hour type the hour
you want and press enter.

6.3.Date/Time
Day: 31

6.3.Date/Time
Month: 1

6.3.Date/Time
Year: 09

6.Install/Service

3.Date/Time?

6.Install/Service

1.Login/Logout?

Phadia 100 Ver.3.0 14:10:17

6.Install/Service?

Phadia 100 Ver.3.0 14:10:17

1.Prepare Run?

6.3.Date/Time
Hour: 14

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1

6.Install/Service

1.Login/Logout?

6.Install/Service

4.System parameters?

6.4.System parameters

1.Language?

6.4.1.Language
Language: ENGLISH?

6.4.1.Language
Time format: 24-HOUR?

6.4.1.Language
Time separator: :

9. If the displayed minute is

correct press enter. If you wish
to change minute type the
minute you want and press
enter.

Language

1. In Install/Service mode

press 4.

2. Press yes.

3. Press yes.

4. Press no until the language

you want appears, then press
yes.

5. Press no until the format

you want appears, then press
yes.

6. Press no until the format

you want appears, then press
yes.

7. Type the character you

want for separator between the
hour and minute and press en-
ter. For more characters press
the shift key and key 0-9 at the
same time.

Default Method

It is possible to choose the method that Phadia 100 displays as a default method
in different functions.

1. In Install/Service mode

press 4.

6.3.Date/Time
Minute: 10

6.4.1.Language
Date format: DAY-MONTH-YEAR?

6.Install/Service

1.Login/Logout?

Instrument Specification and Installation Phadia 100 - User Manual

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6.4.System parameters

2.Default Method?

6.4.2.Default Method
Method: ImmunoCAP Specific IgE 0-100?

6.4.System parameters

1.Language?

6.Install/Service

4.System parameters?

6.Install/Service

1.Login/Logout?

6.Install/Service

5.Contrasts?

2. Press yes.

3. In System Parameters

mode press 2.

4. Press yes.

5. Press no until the method

you want as default appears,
then press yes.

Set sIgE Method

The instrument can be set to use either ImmunoCAP Specific IgE 0-100 or
ImmunoCAP Specific IgE as sIgE method. At delivery ImmunoCAP Specific IgE
0-100 is set as default.

1. In Install/Service mode

press 4.

2. Press yes.

3. In System Parameters

mode press 9.

4. Press yes.

5. Press no until the sIgE

method you want appears, then
press yes.

Contrast

1. In Install/Service mode

press 5.

2. Press yes.

6.Install/Service

1.Login/Logout?

6.4.System parameters

9.Set sIgE Method?

6.4.9.Set sIgE Method
Method: ImmunoCAP Specific IgE 0-100?

6.4.System parameters

1.Language?

6.Install/Service

4.System parameters?

6.4.9.Set sIgE Method
Method: ImmunoCAP Specific IgE?

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NOTE! If you change the LCD contrast too much
is it impossible to read the text presented in the
window.

6.Install/Service

1.Login/Logout?

6.1.Login/Logout

3.Change password?

6.1.Login/Logout

1.Login?

3. Press yes.

4. Change the LCD contrast

by pressing the key below
BRIGHTER or DARKER.
Press enter when the contrast
is set.

5. Press no.

6. Press yes.

7. Change the printer con-

trast by pressing the key below
BRIGHTER or DARKER. The
contrast can be set between 0-

9. Press the key below PRINT
if you want to make a printout
to check the choosen contrast.
Press enter when the contrast
is right.

Set and Change Passwords

You find the settings and changing of passwords in the 6.1.Login/Logout menu.
Default Laboratory Password is "unicap". To change this password to a specific
one known only to authorized personnel, go to 6.Install/Service (as described
in the section Date/Time) and follow the description below.

1. In Install/Service mode

press yes.

2. In Login/Logout mode

press 3.

3. Press yes.

SELECT LCD CONTRAST
BRIGHTER DARKER

6.5.Contrasts

1.Set LCD Contrast?

6.5.Contrasts

2.Set Printer Contrast?

SELECT PRINTER CONTRAST
BRIGHTER DARKER PRINT Contrast:5

6.5.Contrasts

1.Set LCD Contrast?

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6.7.Communication parameters COM2

1.Baud rate?

6.7.1.Baud rate COM2
Baud rate: 9600?

6.7.Communication parameters COM2

1.Baud rate?

6.7.Communication parameters COM2

2.Data bits?

6.7.Communication parameters
Select Port: COM2?

4. Type in the new password,

then press enter.

5. Retype the new password,

then press enter.

6. The new password is set.

Communication Parameters

To enter this menu you need access to the laboratory password. In most cases
you do not need to change the settings for the communication parameters, if you
connect a bar code reader you sometimes need to change the settings. Follow the
steps below when you set the parameters for the bar code reader. To connect a
barcode reader of USB type, please contact your local Phadia representative.

1. In Install/Service mode

press 7.

2. Press yes.

3. Press no until the com-

munication port that you want
to change parameters for is dis­played.

Instruments equipped with USB ports:

Choose COM2 to connect a bar code reader to the RS-232 port.

Instruments equipped with a diskette drive:

Choose COM3 to connect a bar code reader to the RS-232 port.
Confirm with yes.

4. Press yes.

5. Press no until the baud

rate you want appears, then
press yes.

6. Press no.

7. Press yes.

6.1.3.Change Password
New password:_

6.1.3.Change Password
Confirm password:_

6.1.3.Change Password
NEW PASSWORD SET

6.Install/Service

7.Communication parameters?

6.Install/Service

1.Login/Logout?

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6.7.5.Port Usage COM2
Select Usage for COMPORT: BARCODE?

8. Press no until the data bits

you want appear, then press
yes.

9. Press no.

10. Press yes.

11. Press no until the stop bits

you want appear, then press
yes.

12. Press no.

13. Press yes.

14. Press no until the parity

you want appears, then press
yes.

15. Press no.

16. Press yes.

17. Press no until BARCODE

appears, then press yes.

Configure Phadia 100 for TCP/IP

When using IDM the IP address and the Port for IDM has to be set. To enter this
menu you need access to the laboratory password. Follow the steps below when
you set the IP address and Port.

1. In Install/Service mode

press 7.

2. Press yes.

3. Press no until 6 is dis-

played.

6.7.Communication parameters COM2

4.Parity?

6.7.Communication parameters COM2

5.Port Usage?

6.7.3.Stop bits COM2
Stop bits: 1?

6.7.Communication parameters COM2

3.Stop bits?

6.7.Communication parameters COM2

3.Stop bits?

6.7.Communication parameters COM2

4.Parity?

6.7.4.Parity COM2
Parity: NONE?

6.7.Communication parameters COM2

2.Data bits?

6.7.2.Data bits COM2
Data bits: 8?

6.Install/Service

1.Login/Logout?

6.Install/Service

7.Communication parameters?

6.7.Communication parameters COM2

6.IDM TCP/IP Address?