Thermo Scientific PerpHecT ROSS, ROSS, ROSS Ultra Triode, Orion ROSS Ultra User Manual
User Guide
Thermo Scientific
Orion ROSS Ultra®,
ROSS Ultra Triode™,
ROSS® and
PerpHecT® ROSS
Electrodes
ROSS and the COIL trade dress are trademarks of Thermo Fisher Scientific Inc.
U.S. patent 6,793,787.
AQUAfast, Cahn, ionplus, KNIpHE, No Cal, ORION, perpHect, PerpHecT,
PerpHecTion, pHISA, pHuture, Pure Water, Sage, Sensing the Future,
SensorLink, ROSS, ROSS Ultra, Sure-Flow, Titrator PLUS and TURBO2 are
registered trademarks of Thermo Fisher.
1-888-pHAX-ION, A+, All in One, Aplus, AQUAsnap, AssuredAccuracy,
AUTO-BAR, AUTO-CAL, AUTO DISPENSER, Auto-ID, AUTO-LOG, AUTO-READ,
AUTO-STIR, Auto-Test, BOD AutoEZ, Cable-Free, CERTI-CAL, CISA, DataCOLLECT,
DataPLUS, digital LogR, DirectCal, DuraProbe, Environmental Product
Authority, Extra Easy/Extra Value, FAST QC, GAP, GLPcal, GLPcheck, GLPdoc,
ISEasy, KAP, LabConnect, LogR, Low Maintenance Triode, Minimum Stir
Requirement, MSR, NISS, One-Touch, One-Touch Calibration, One-Touch
Measurement, Optimum Results, Orion Star, Pentrode, pHuture MMS, pHuture
Pentrode, pHuture Quatrode, pHuture Triode, Quatrode, QuiKcheK, rf link,
ROSS Resolution, SAOB, SMART AVERAGING, Smart CheK, SMART STABILITY,
Stacked, Star Navigator 21, Stat Face, The Enhanced Lab, ThermaSense,
Triode, TRIUMpH, Unbreakable pH, Universal Access are trademarks of
Thermo Fisher.
Guaranteed Success and The Technical Edge are service marks of
Thermo Fisher.
PerpHecT meters are protected by U.S. patent 6,168,707.
PerpHecT ROSS electrodes are protected by U.S. patent 6,168,707.
ORION Series A meters and 900A printer are protected by U.S. patents
5,198,093, D334,208 and D346,753.
ionplus electrodes and Optimum Results solutions are protected by
U.S. patent 5,830,338.
ROSS Ultra electrodes are protected by U.S. patent 6,793,787.
ORP standard is protected by U.S. patent 6,350,367.
No Cal electrodes are protected by U.S. patent 7,276,142.
© 2009 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are
the property of Thermo Fisher Scientific Inc. and its subsidiaries.
The specifications, descriptions, drawings, ordering information and part
numbers within this document are subject to change without notice.
This publication supersedes all previous publications on this subject.
Table of Contents
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
ROSS Electrode Families . . . . . . . . . . . . . . . . . . . . . . . . . . .2
Required Equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
Electrode Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
Preparation for Refillable ROSS Electrodes . . . . . . . . . . . . . . . . . 4
Preparation for ROSS Sure-Flow Electrodes . . . . . . . . . . . . . . . .5
Preparation for Gel-Filled ROSS Ultra Triode Electrodes . . . . . . .6
Sample Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
Measuring Hints . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
Electrode Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
General Calibration Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . 10
One Buffer Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
Two Buffer Calibration. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
pH Measurements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Electrode Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Electrode Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . .12
Electrode Cleaning Procedures . . . . . . . . . . . . . . . . . . . .13
General Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Cleaning Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Electrode Characteristics . . . . . . . . . . . . . . . . . . . . . . . . .14
Temperature Effects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Sure-Flow Electrode Leak Rate . . . . . . . . . . . . . . . . . . . . . . . . . 15
PerpHecT ROSS Micro pH Electrode . . . . . . . . . . . . . . . . . . . . . 15
ROSS Ultra pH/ATC Triode Electrodes . . . . . . . . . . . . . . . . . . . . 15
PerpHecT® ROSS® Electrode Operation with
PerpHecT® pH Meters . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Electrode Interferences . . . . . . . . . . . . . . . . . . . . . . . . . .
17
Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
Assistance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .19
RoHS Statement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .20
Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
21
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
IThermo Scientific Orion ROSS® Electrode User Guide
II Thermo Scientific Orion ROSS® Electrode User Guide
Introduction
This user guide contains information on the preparation,
operation and maintenance of the Thermo Scientific Orion ROSS
Ultra, ROSS and PerpHecT ROSS electrodes.
All ROSS pH electrodes have a pH range of 0 to 14, pH precision
of 0.01 and temperature range of 0 to 100 °C. ROSS electrodes
can be used in samples that contain TRIS, sulfides and proteins
since they do not contain silver or mercury.
ROSS electrodes incorporate the patented ROSS internal
reference system that provides superior measurement stability,
faster response, greater accuracy and more reproducible results
than conventional electrodes. ROSS electrodes have a double
junction reference, so the fill solution can be modified to more
closely match the sample composition in high pH, low pH or
non-aqueous solutions.
ROSS electrodes provide readings stable to 0.01 pH in less
than 30 seconds, even in the extreme case of samples varying
from one another by 50 °C or more. Results are three to five
times more precise than those obtained with conventional
electrodes. Electrode drift is less than 0.002 pH per day, so
recalibration is minimized.
• All electrodes with a waterproof BNC, BNC or U.S. standard
connector have one meter cable lengths, except for the
8107UWMMD and 8157UWMMD electrodes that have a
three meter cable length.
• All electrodes with a screw cap connection require a
separate cable.
• Electrodes with a waterproof BNC connector can be used
on any pH meter with a BNC connection.
• The 800500U ROSS Ultra reference half-cell and 800300
ROSS reference half-cell can be used with the 8101BNWP
and 8101SC ROSS pH half-cell electrodes or 8411BN ROSS
sodium half-cell electrode.
1Thermo Scientific Orion ROSS® Electrode User Guide
ROSS Electrode Families
ROSS Ultra® Electrodes
ROSS Ultra electrodes offer the ultimate measurement stability
and the least long-term drift plus a two year warranty.
ROSS Ultra® pH/ATC Triode® Electrodes
ROSS Ultra pH/ATC Triodes have a built-in temperature probe,
so pH and temperature are measured with one electrode.
Gel-filled ROSS Ultra pH/ATC Triode electrodes have an 18
month warranty and refillable ROSS Ultra pH/ATC Triode
electrodes have a two year warranty.
ROSS® Electrodes
ROSS electrodes provide precise pH measurements and
measurement stability regardless of temperature changes or
sample composition.
ROSS® Sure-Flow® Electrodes
ROSS Sure-Flow electrodes incorporate a free-flowing junction
that is easy to clean and never clogs. These electrodes are ideal
for dirty or viscous samples that clog traditional electrodes.
PerpHecT® ROSS® Electrodes
PerpHecT ROSS electrodes are designed to be used with
PerpHecT meters to provide both pH and temperature
measurements. The PerpHecT meters use patented LogR
technology that allows temperature to be measured using the
resistance of the PerpHecT ROSS electrode.
2 Thermo Scientific Orion ROSS® Electrode User Guide
Required Equipment
1. Thermo Scientific Orion pH meter, such as the 3-Star pH
meter, 4-Star pH/ISE meter, 4-Star pH/DO meter, 4-Star pH/
conductivity meter or 5-Star pH/ISE/DO/conductivity meter.
ROSS electrodes can be used on any pH meter with a BNC
or U.S. standard connection. The electrodes can also be
used on meters with a variety of inputs when an adapter
cable is used. Visit www.thermo.com/water for details.
2. Thermo Scientific Orion ROSS Ultra, ROSS or PerpHecT
ROSS electrode.
• The 8302BNUMD, 8107BNUMD, 8107UWMMD,
8157BNUMD and 8157UWMMD pH/ATC Triodes have
a temperature connector that is compatible with the
Star series pH meters.
• The 8107BNU and 8157BNU pH/ATC Triodes have a
temperature connector that is compatible with the
A+ series meters.
• The 8207BNU and 8257BNU pH/ATC Triodes have a
temperature connector that is compatible with the
PerpHecT meters.
3. ROSS fill solution, Cat. No. 810007.
Note: Do not use any fill solution that contains silver, as
silver will damage ROSS electrodes.
Note: The 8107BNU, 8107BNUMD, 8107UWMMD and
8207BNU electrodes are gel-filled and do not require any
fill solution.
4. ROSS pH electrode storage solution, Cat. No. 810001, or
standard pH electrode storage solution, Cat. No. 910001.
For enhanced electrode performance, use ROSS pH
electrode storage solution.
5. pH buffers – at least two pH buffers are recommended for
precise measurements. One buffer should be near pH 7 and
buffers should be one to four pH units apart.
6. Beakers, plastic or glass.
7. Magnetic stirrer or Thermo Scientific Orion stirrer probe,
Cat. No. 096019. The stirrer probe can be used with 3-Star,
4-Star and 5-Star benchtop meters.
8. Distilled or deionized water.
3Thermo Scientific Orion ROSS® Electrode User Guide
Electrode Preparation
Fill hole
ROSS
internal
reference
Fill
solution
chamber
Reference
junction
pH sensing
bulb
Preparation for Refillable ROSS Electrodes
1. Remove the shipping cap, sleeve or bottle from the sensing
bulb and save it for later storage of the electrode.
2. Clean any salt deposits from the exterior of the electrode
by rinsing it with distilled water. Handle the 8220BNWP
PerpHecT ROSS micro pH electrode with great care.
3. Uncover the fill hole by removing the tape and add ROSS
fill solution, Cat. No. 810007, to the electrode up to the
fill hole. To maintain an adequate flow rate, the level of fill
solution must always be above the reference junction and
at least one inch above the sample level. The fill hole should
remain open whenever the electrode is in use.
4. Place the electrode in an electrode holder and suspend
the electrode in air for 5 minutes to thoroughly wet the
reference junction. The 8220BNWP electrode and ROSS
Ultra Triode electrodes do not need to be suspended in air.
5. Shake the electrode downward (similar to a clinical
thermometer) to remove any air bubbles. Do not shake the
8220BNWP electrode unless there are air bubbles in the fill
solution and then shake gently and with great care.
6. Soak electrode in ROSS pH electrode storage solution,
Cat. No. 810001, or standard pH electrode storage solution,
Cat. No. 910001, for 30 to 60 minutes. ROSS Ultra Triode
electrodes do not need to be soaked prior to use, since
they are shipped in a storage bottle with storage solution.
7. Connect the electrode to the meter.
ROSS Refillable Combination pH Electrode
4 Thermo Scientific Orion ROSS® Electrode User Guide
Preparation for ROSS Sure-Flow Electrodes
Fill hole
ROSS
internal
reference
Fill
solution
chamber
Reference
junction
pH sensing
bulb
Fill hole
ROSS
internal
reference
Fill solution
chamber
Sure-Flow
reference
junction
pH sensing
bulb
1. Remove the shipping cap, sleeve or bottle from the sensing
bulb and save it for later storage of the electrode.
2. Clean any salt deposits from the exterior of the electrode by
rinsing it with distilled water.
3. Uncover the fill hole by removing the tape and add ROSS
fill solution, Cat. No. 810007, to the electrode up to the
fill hole. To maintain an adequate flow rate, the level of fill
solution must always be above the reference junction and
at least one inch above the sample level. The fill hole should
remain open whenever the electrode is in use.
4. Wet the junction by pressing down on the electrode cap
to let a few drops of the ROSS fill solution flow out of the
electrode and then replenish any lost fill solution.
5. Shake the electrode downward (similar to a clinical
thermometer) to remove any air bubbles.
6. Soak electrode in ROSS pH electrode storage solution,
Cat. No. 810001, or standard pH electrode storage solution,
Cat. No. 910001, for 30 to 60 minutes. For enhanced
electrode performance, use ROSS pH electrode
storage solution.
7. Connect the electrode to the meter.
ROSS Sure-Flow Combination pH Electrode
5Thermo Scientific Orion ROSS® Electrode User Guide
pH sensing bulb
Reference junction
ROSS
internal
reference
Gel solution
chamber
Preparation for Gel-Filled ROSS Ultra
Triode Electrodes
1. Remove the electrode storage bottle from the sensing bulb
and save it for later storage of the electrode.
Note: Unscrew the cap on the electrode storage bottle
before removing the electrode.
2. Clean any salt deposits from the exterior of the electrode by
rinsing it with distilled water.
3. Connect the electrode to the meter.
4. If the electrode will not be used immediately, soak it in
ROSS pH electrode storage solution, Cat. No. 810001, or
standard pH electrode storage solution, Cat. No. 910001.
ROSS Gel-filled Combination pH Electrode
6 Thermo Scientific Orion ROSS® Electrode User Guide
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