Qubit™ Flex Fluorometer
USER GUIDE
Catalog Number A44984, A45924, A45925
Publication Number MAN0018186
Revision A.0
For Research Use Only. Not for use in diagnostic procedures.
Manufacturer: Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
Information in this document is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH
OR ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: MAN0018186
Revision Date Description
A.0 11 October 2019 New user guide.
Important Licensing Information: This product may be covered by one or more Limited Use Label Licenses. By use of this product,
you accept the terms and conditions of all applicable Limited Use Label Licenses.
Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is
a registered trademark of Kimberly-Clark Corporation. Windows is a registered trademark of Microsoft Corporation.
© 2019 Thermo Fisher Scientific Inc. All rights reserved.
ii Qubit
™
Flex Fluorometer User Guide
Contents
About this guide ........................................................................................................... 3
1. Product information ............................................................................................... 5
Product contents .............................................................................................................................................. 5
Product description ......................................................................................................................................... 6
Instrument exterior components ................................................................................................................... 7
Product specifications ..................................................................................................................................... 8
2. Getting started ...................................................................................................... 10
Set up the Qubit™ Flex Fluorometer............................................................................................................ 10
Connect to the network ................................................................................................................................ 12
After instrument setup ................................................................................................................................. 14
Sign in ............................................................................................................................................................. 16
Guidelines for using the Qubit™ Flex Fluorometer ................................................................................... 23
3. Perform assays ..................................................................................................... 24
Before you begin ............................................................................................................................................ 24
(Optional) Use the Assay Range Calculator to determine the assay range ............................................ 26
Use the Reagent Calculator to prepare Qubit™ Working Solution ......................................................... 30
Run standards for assay calibration ............................................................................................................ 32
Read samples ................................................................................................................................................. 36
Results ............................................................................................................................................................. 43
(Optional) Use the Molarity Calculator to determine sample molarity .................................................. 45
(Optional) Use the Normalization Calculator to determine how to dilute the samples to the
same molarity, concentration, or mass ....................................................................................................... 48
4. Manage data ......................................................................................................... 58
View detailed sample data ........................................................................................................................... 58
Edit sample name .......................................................................................................................................... 63
Export data ..................................................................................................................................................... 66
Delete data ...................................................................................................................................................... 70
5. Configure instrument settings .............................................................................. 72
Instrument settings ........................................................................................................................................ 72
Sleep mode ..................................................................................................................................................... 73
Brightness ....................................................................................................................................................... 74
Date and Time ................................................................................................................................................ 75
Network connection ...................................................................................................................................... 78
Reset instrument ............................................................................................................................................ 84
Language ........................................................................................................................................................ 85
Cloud region .................................................................................................................................................. 86
Qubit™ Flex Fluorometer User Guide 1
6. Instrument maintenance ...................................................................................... 87
Maintenance and cleaning............................................................................................................................ 87
Software updates ........................................................................................................................................... 88
System verification ........................................................................................................................................ 90
Replace battery .............................................................................................................................................. 95
Appendix A: Troubleshooting .................................................................................... 97
Troubleshooting ............................................................................................................................................. 97
Critical Qubit™ Assay considerations ......................................................................................................... 99
Appendix B: Ordering information .......................................................................... 101
Qubit™ Flex Fluorometer and accessories ................................................................................................ 101
Appendix C: Safety .................................................................................................. 102
Symbols on instruments ............................................................................................................................. 102
Safety labels on instruments ...................................................................................................................... 103
General instrument safety .......................................................................................................................... 104
Chemical safety ............................................................................................................................................ 105
Chemical waste safety................................................................................................................................. 106
Electrical safety ............................................................................................................................................ 107
Biological hazard safety .............................................................................................................................. 108
Safety, Electromagnetic compatibility (EMC), and Environmental standards ................................... 109
Documentation and support ................................................................................... 111
Obtaining support ....................................................................................................................................... 111
2 Qubit
™
Flex Fluorometer User Guide
Overview
This user guide describes how to operate the Qubit™ Flex Fluorometer.
User
documentation
The guides listed below are available with the Qubit™ Flex Fluorometer.
to access protocols, application notes, and tutorials.
Text and keyboard
Text and keyboard conventions used in the Qubit™ Flex Fluorometer User Guide are
user documentation, see page 4.
User attention
words
Two user attention words appear in Thermo Fisher Scientific user documentation.
About this guide
Guide Pub. No.
Qubit™ Flex Fluorometer User Guide
Qubit™ Flex Fluorometer Quick Reference Card (QRC)
Additional resources are available on the Qubit™ Technical Resources page. Go to
thermofisher.com/qubit
MAN0018186
MAN0018187
conventions
listed below. For safety alert words and symbols used in Thermo Fisher Scientific
Convention Use
Bold
Right arrow symbol () indicates a menu choice, and
Each word implies a particular level of observation or action as described below.
Bold text indicates user action. For example:
Click Run.
separates successive commands you execute or select from a
drop-down or shortcut menu. For example:
Select SettingsInstrument Settings.
Note: Provides information that may be of interest or help but is not
critical to the use of the product.
IMPORTANT! Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
Qubit™ Flex Fluorometer User Guide 3
Safety alert words
Four safety alert words appear in Thermo Fisher Scientific user documentation at
points in the document where you need to be aware of relevant hazards. Each
particular level of observation or action, as defined below:
Except for IMPORTANT! safety alerts, each safety alert word in a Thermo Fisher
to Thermo Fisher Scientific instruments (see “Safety symbols” in Appendix C).
SDSs
The Safety Data Sheets (SDSs) for any chemicals supplied by Thermo Fisher
SDSs, see “Safety Data Sheets (SDS)”.
alert word—IMPORTANT, CAUTION, WARNING, DANGER—implies a
IMPORTANT! – Provides information that is necessary for proper
instrument operation, accurate installation, or safe use of a chemical.
CAUTION! – Indicates a potentially hazardous situation that, if not
avoided, may result in minor or moderate injury. It may also be used to
alert against unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.
Scientific document appears with an open triangle figure that contains a hazard
symbol. These hazard symbols are identical to the hazard symbols that are affixed
Scientific are available to you free 24 hours a day. For instructions on obtaining
IMPORTANT! For the SDSs of chemicals not distributed by Thermo
Fisher Scientific contact the chemical manufacturer.
4 Qubit
™
Flex Fluorometer User Guide
The Qubit™ Flex Fluorometer (Cat. No. A44984) is shipped with the following
components:
The complete user guide is available for download at thermofisher.com/qubit.
See page 6 for the description and specifications of the Qubit™ Flex Fluorometer.
Upon receiving the
Examine the instrument carefully for damage incurred during transit. Ensure that
See page 10 for instructions to set up the Qubit™ Flex Fluorometer.
Register your
Go to thermofisher.com/qubit to register your instrument. You will be asked to
information on new assays for use with the Qubit™ Flex Fluorometer.
Product contents
Qubit™ Flex Fluorometer 1 each
Qubit™ Flex power cord (shipped separately)
USB drive 1 each
Qubit™ Flex LAN cable 1 each
Qubit™ Flex Fluorometer Quick Reference Card (QRC) 1 each
Certificate of Conformity (COC) 1 each
Qubit™ screen cleaning cloth 1 each
Wi-Fi Dongle 1 each
[1]
The power cords for the Qubit™ Flex Fluorometer are not interchangeable with those for
the other Qubit™ Fluorometer models. Powering the instrument with an unapproved power
cord can irreversibly damage the instrument.
1. Product information
Component Quantity
[1]
1 each
instrument
instrument
all parts of the instrument, including the accessories listed above, are included with
the product. Damage claims must be filed with the carrier; the warranty does not
cover in-transit damage.
supply the serial number, your name, and your contact details. Registering your
instrument ensures that you will receive notifications of software upgrades and
Qubit™ Flex Fluorometer User Guide 5
Product description
Qubit™ Flex
The Qubit™ Flex Fluorometer is a benchtop fluorometer for the quantification of
highly sensitive and accurate fluorescence-based Qubit™ assays.
Features
•
Fluorometer
DNA, RNA, microRNA, and protein. With the Qubit™ Flex Fluorometer, you can
directly measure the fluorescence of up to 8 samples simultaneously using the
Fast and highly accurate quantification of DNA, RNA, and protein of up to
8 samples simultaneously in ~3 seconds.
• High levels of accuracy using only 1–20 µL of sample, even with very dilute
samples.
• Use of dyes selective for dsDNA, RNA, or protein minimizes the effects of
contaminants in the sample.
• Stores results from up to 10,000 samples.
• 8-inch, state-of-the-art color touchscreen for easy workflow navigation.
• Instrument indicates samples that are in the extended range or out of range.
• Saves sample data as a CSV (comma separated value) file.
• On-board Reagent and Range Calculators provide instructions to prepare
™
Qubit
working solution using your sample and standard inputs and to select
the most accurate assay for your expected concentration range.
• On-board Molarity and Normalization Calculators allow you to calculate
molarity of your samples based on nucleic acid length and determine how to
dilute the samples to the same concentration, respectively, using the results
from your assays.
• Allows easy definition and saving of assay preferences.
™
• Exports data to a USB drive, to a network drive, or to the Connect
cloud-based
platform.
• Connects to the local area network via the LAN (RJ-45) port using an Ethernet
cable or wirelessly using the supplied Wi-Fi adaptor.
• Instrument user interface can be personalized to display in the language of your
choice including English, French, Spanish, Italian, German, simplified Chinese
and Japanese.
6 Qubit
™
Flex Fluorometer User Guide
Top view
Rear view
Instrument exterior components
Qubit™ Flex Fluorometer User Guide 7
Touchscreen is the user interface containing the controls for all the functions
needed and displays data from the assays.
Sample chamber is used to load the Qubit™ Flex Tube Strip containing your
samples into the fluorometer for analysis.
USB drive ports (Type A) allow you to transfer and save data to your computer
™
using a USB flash drive or wirelessly to a network drive or a Connect
account
using the Wi-Fi dongle (supplied with the instrument).
Power inlet connects the Qubit™ Flex Fluorometer to an electrical outlet using
the supplied power cord and the appropriate plug.
LAN port (RJ-45) allows you to connect to the network using an Ethernet cable.
Product specifications
Physical
Instrument type: Benchtop fluorometer
Electrical input: 48 VDC, 1.87 A
Operating
Installation site: Indoor use only
areas.
Technical
Warm-up time: <35 seconds
characteristics
conditions
Instrument dimensions: 7.3 in (w) × 11.1 in (l) × 4.1 in (h)
(18.6 cm × 28.2 cm × 10.3 cm); rectangular shape
Weight: 60 oz. (1.7 kg)
Operating power: 100–240 ±10% VAC, 1.3 A
Frequency: 50/60 Hz
IMPORTANT! If the supplied power fluctuates ±10% beyond the rated
voltage, a power line regulator may be required. High or low voltages can
adversely affect the electronic components of the instrument.
Altitude: Between sea level and 2000 m (6500 ft.) above
sea level
Operating temperature: 10–30°C
Operating humidity: 15–80% (non-condensing)
Pollution degree: The instrument has a Pollution Degree rating of II.
The instrument may only be installed in an
environment that has nonconductive pollutants.
Typical environment with a Pollution Degree II
rating are laboratories and sales and commercial
Dynamic range: 4 orders of magnitude
specifications
Processing time: ≤3 seconds/sample
Light sources: Blue LED (max 460–480 nm)
Red LED (max 620–640 nm)
Excitation filters: Blue 456–484 nm
Red 612–644 nm
Emission filters: Green 513–563 nm
Far-Red 671–693 nm
Detectors: Photodiodes; measurement capability from
320–1100 nm
Calibration type: 2- or 3-point standard
Sample chamber: Accommodates one Qubit
Tube type: Qubit
polypropylene tubes; Cat. No. Q33252)
™
Flex Tube Strip
™
Flex Tube Strip (8× 0.2-mL thin-wall
8 Qubit
™
Flex Fluorometer User Guide
Hardware
Display: 8-inch capacitive touchscreen with high resolution
USB drive
Capacity: 4 Gigabyte
color display
Output ports: 3× USB ports
Networking capability: Connection via the LAN (RJ-45) port using an
Ethernet cable or wirelessly using the supplied
Wi-Fi adaptor
Power supply: AC adaptor with country‐specific power cords
Qubit™ Flex Fluorometer User Guide 9
2. Getting started
Install the
The Qubit™ Flex Fluorometer is a stand-alone instrument that does not require
plug remains accessible to allow disconnection.
5. The instrument automatically powers on, first displaying the splash screen,
6. Click Accept to accept the terms of the agreement and proceed to “Set
language and date/time options” (page 11).
7. To power down the Qubit™ Flex Fluorometer, unplug it.
Set up the Qubit™ Flex Fluorometer
instrument
connection to a computer.
1. After unpacking the instrument, place the instrument on a flat, level, dry
surface.
2. Plug one end of the supplied power cord into the Qubit
™
Flex Fluorometer.
3. Attach the appropriate plug adaptor to the other end of the power cord.
4. Plug the power cord into the electrical outlet. Ensure that the power adaptor
IMPORTANT! Use the power cord plug adapter supplied with the
instrument that is appropriate for the electrical outlet configuration in
your country. Powering the instrument with an unapproved power
cord can irreversibly damage the instrument. Note that the power
™
cords for the Qubit
those for the other Qubit
Flex Fluorometer are not interchangeable with
™
Fluorometer models.
then the End User License Agreement (EULA) screen.
Note: The End User License Agreement (EULA) screen is displayed on
the first use of the instrument. On subsequent uses, the Home screen
(page 14) is displayed after the splash screen.
Note: You can also view and export the EULA from the About
Instrument screen (page 15).
10 Qubit
™
Flex Fluorometer User Guide
Set language and
After you accept the EULA, the instrument shows the Language displayed and
date/time options
Date/Time screens, which allow you to set language and date/time options. If you
wish, you can later change the language settings from the SettingsInstrument
SettingsLanguage screen (page
85).
1. On the Language displayed screen, select the Language you want your
instrument to display, then press Next.
Available options are English, French, German, Italian, Chinese, Japanese,
and Spanish.
2. On the Date/Time screen, select the Time Zone, set the Date and Time in the
desired format, then press Next.
Note: For detailed instructions on how to configure date/time options
and to set the date and time, see “Set the date and time”, page 75.
Qubit™ Flex Fluorometer User Guide 11
Connect to the network
(Optional)
Connect
After you set language and date/time options, the instrument displays the
to the network
Network Connection screen, which allows you to configure network options. If
you wish, you can skip this step and connect to the network later from the
SettingsInstrument settingsNetwork connection screen (page
1. On the Network Connection screen, select Wireless or Wired connection.
If you wish to use the instrument without joining a network, press Skip. You
can always join a network and configure network settings later.
78).
2. Depending on your choice, the instrument displays the Choose Network or
the IP Configuration screen (for Wireless and Wired connection, respectively).
Choose Network screen
(for Wireless connection)
IP Configuration screen
(for Wired connection)
12 Qubit
™
Flex Fluorometer User Guide
3. For wireless connection, select the network you want to join, then follow the
on-screen instructions to configure the network options. When finished, press
Join.
For wired connection, configure the network connection options, then press
Done.
For detailed instructions on how to join a network (wireless or wired) and
configure network options, see “Network connection”, page 78.
4. On the Network Connection screen, click Network Drive to map the location
on the network where you want to save your Qubit
™
Flex files.
For detailed instructions on how to map a network drive, see “Map a network
drive”, page 81.
Note: You must have an established network connection to map a network
drive. If you wish, you can to map the network drive later.
Qubit™ Flex Fluorometer User Guide 13
After instrument setup
Home screen
After you have set instrument preferences, the instrument automatically displays
Use the Range Calculator to determine the best assay to use for your sample.
the Home screen each time it is powered on.
From the Home screen, you can:
• Sign in to your local instrument profile or your Connect
• Select the assays to perform:
- 1X dsDNA High Sensitivity (HS)
- dsDNA High Sensitivity (HS)
- dsDNA Broad Range (BR)
- RNA High Sensitivity (HS)
- RNA Broad Range (BR)
- Protein
- Oligo (ssDNA)
- microRNA
• Access saved data.
• Filter, delete, or export data.
• Configure instrument settings.
• Use the Reagent Calculator to determine the exact volumes of Qubit
and reagent required to prepare the Qubit
•
™
account.
™
working solution.
™
buffer
14 Qubit
™
Flex Fluorometer User Guide
About Instrument
The About Instrument screen displays information about your Qubit™ Flex
3. Press Close or Back ( ) to return to the Settings screen.
screen
Fluorometer, including the currently installed software version.
To access the About Instrument screen:
1. On the Home screen, press Settings.
2. On the Settings screen, press About Instrument to display the About
Instrument screen.
Qubit™ Flex Fluorometer User Guide 15
Sign in
Create a local
Qubit™ Flex Fluorometer allows you to create a local instrument profile for each
instrument profile
user. A local instrument profile allows you to save to a mapped network location
and it is also required to connect to your Connect
skip this step and create a profile later from the Profile screen.
1. On the Home screen, press the Profile button on the top left corner of
the screen to open the Sign In screen.
™
account. If you wish, you can
2. If you are new to the instrument and have not yet created a profile, press
Get Started to open the Get Started screen, then press Create Profile.
16 Qubit
™
Flex Fluorometer User Guide
3. On the Create Profile screen, press Create.
If you wish to use the instrument without creating a local profile, press Skip.
You can always create an instrument profile later.
4. Press the User name field, enter the desired user name for the profile (1−20
alphanumeric characters, no spaces), then press Done.
5. Press the PIN field, enter a 4-digit PIN, then press Done.
6. Enter the PIN in the Confirm PIN field, then press Done.
7. Press Create to create the local instrument profile.
Qubit™ Flex Fluorometer User Guide 17
Sign in to your
Connect™ account
After you have joined a network, you can also connect to your Connect™ account,
1. Ensure that you are connected to the network on your Qubit™ Flex instrument
Thermo Fisher’s cloud-based platform, to store and access your data files.
Note: To connect to the Thermo Fisher Cloud, you must have a Connect™
account or create one. To create your Connect
to your existing account, go to thermofisher.com/cloud.
(page 12).
2. On the Home screen, press the Profile button on the top left corner of
the screen to open the Sign In dialog.
™
account online or to sign in
3. On the Sign In screen, press Get Started to open the Get Started screen, then
press Connect to open the Connect to the Cloud screen.
18 Qubit
™
Flex Fluorometer User Guide
4. Connect to the Cloud screen offers three methods to sign in to your Thermo
Fisher Connect™ account:
• Instrument Connect App on your
mobile phone (Step 5, page 19)
• Dashboard on PC (Step 6, page 20)
• Instrument (Manually) (Step 7,
page 21)
5. To connect to your Thermo Fisher Connect™ account with the Instrument
Connect App on your mobile phone:
a. Download the Instrument Connect Mobile App from the application
store on your mobile phone.
b. Press Instrument Connect App on the Connect to the Cloud screen, then
follow the steps on the Qubit
Step 8 (page 22).
™
Flex instrument. When finished, go to
Qubit™ Flex Fluorometer User Guide 19
6. To connect to your Thermo Fisher Connect™ account with Dashboard on PC:
a. Go to thermofisher.com/cloud and sign in to your Thermo Fisher
™
Connect
b. On the Connect
account.
™
dashboard, press the Instrument Connect button.
Instrument Connect screen opens.
c. On the Instrument Connect screen, press
Add an instrument.
Add an instrument dialog opens.
d. From the instrument type dropdown, select Qubit_Flex, then press Next.
20 Qubit
™
Flex Fluorometer User Guide
e. Press Dashboard on PC on Connect to the Cloud screen (on the Qubit™
7. To connect to your Thermo Fisher Connect™ account with manually with the
Flex instrument; see page 19) to display the linking code.
f. Enter the linking code displayed on the Qubit
™
Flex instrument into the
Add an instrument dialog, then press Send.
g. When finished, go to Step 8 (page 22).
Qubit™ Flex instrument:
a. Press Instrument (Manually) on the Connect to the Cloud screen (on the
™
Qubit
b. Enter your User name and Password for your Thermo Fisher Connect
Flex instrument; see page 19).
account, then press Connect.
™
Qubit™ Flex Fluorometer User Guide 21
8. When you have signed in to your Thermo Fisher Connect™ Account,
the Profile button on the Home screen becomes blue.
When signed in, you can export your data to your Connect
™
account.
22 Qubit
™
Flex Fluorometer User Guide
Recommendations
To obtain the best results, follow the recommendations below. For more
been tested using the Qubit™ Flex Fluorometer.
Assay tubes for
Fluorometer
Only thin-wall, clear 0.2-mL PCR tube strips are appropriate for use in the Qubit™
Guidelines for using the Qubit™ Flex Fluorometer
information, see “Critical Qubit™ Assay considerations”, page 99.
• Do not operate the instrument in direct sunlight.
• Wear gloves during sample handling.
• Use the instrument at room temperature only (22–28ºC).
• Bring all kit reagents to room temperature and insert all assay tubes into the
instrument only for as much time as it takes for the instrument to measure the
fluorescence.
• Do not hold the assay tubes in your hand before performing a measurement.
• Make sure that you have calibrated the Qubit
appropriate standards.
• The assay volume must be 200 µL for an accurate read.
• Take care not to create air bubbles when mixing the sample or standard with
the working solution.
• Incubate the tubes for the Qubit
mixing the sample or standard with the working solution.
• Incubate the tubes for the Qubit
the sample or standard with the working solution.
• If you are performing multiple readings of a single tube, remove the tube
from the instrument and let it equilibrate to room temperature for 30 seconds
before taking another reading.
Note: Multiple readings of RNA samples are not recommended.
• Visit thermofisher.com/qubit
citations, software updates, and a list of validated Qubit
™
Flex Fluorometer using the
™
DNA and RNA assays for 2 minutes after
™
protein assays for 15 minutes after mixing
for additional application notes, technical notes,
™
assays that have
the Qubit™ Flex
Flex Fluorometer. For best results, we recommend using Qubit™ Flex Tube Strips
(Cat. No. Q33252).
Qubit™ Flex Fluorometer User Guide 23
Before you begin
Materials needed
Qubit™ Flex Reservoir (Cat. No. Q33253) or other suitable sample reservoir
•
with the instrument or available separately
Sign in to your
1. Press the Profile button on the top left corner of the screen to open the
Otherwise, go to Step 3 (page 25).
• A Qubit™ assay kit appropriate for quantifying your samples (see page 101 for
• DNA, RNA, or protein samples in Qubit
• Appropriate standards for your assay in Qubit
• Single channel pipette (1–20 µL), multichannel pipette (200 µL)
•
3. Perform assays
™
available Qubit
Note: For instructions on the preparation of the assay standards, see
the instructions that accompany the assay you are using or the Qubit
Flex Fluorometer Quick Reference Card (QRC) (Pub. No. MAN0018187).
(Optional) USB drive, USB cable, or Ethernet cable for data transfer, supplied
Note: You can also transfer your data to a network location or your
Connect
assay kits and ordering information)
™
Flex Tube Strips
™
Flex Tube Strips
™
account wirelessly, if you have set up a wireless connection.
™
profile
Sign In dialog.
2. If you are new to the instrument and have not yet created a
local instrument profile or signed in to your Thermo Fisher
Connect
™
account, press Get Started.
To create a local instrument profile, see page 16.
To sign in to your Thermo Fisher Connect
™
account, see page 18.
24 Qubit
™
Flex Fluorometer User Guide
3. Press Screen name, then select your instrument
4. Press PIN, enter the PIN for your profile, then
profile from the available options.
Note: The Connect icon next to a screen name indicates that the
profile has an associated Connect
™
account.
When the Connect icon is blue, the profile is signed in to the
™
associated Connect
account.
press Enter.
5. Press Sign in to sign in to your account and return to the Home screen.
The blue profile button indicates that you have signed in to your account.
Qubit™ Flex Fluorometer User Guide 25
(Optional)
The on-board Assay Range Calculator displays the core sample concentration
based on your sample volume and estimated sample concentration.
Use the Assay
1. On the Home screen, press Calculators.
range
Use the Assay Range Calculator to determine the assay
range for which the selected assay is most accurate, as well as the extended low
and high ranges based on your sample volume. Knowing the assay range can help
you determine which Qubit
™
assay provides the most accurate quantification
Range Calculator
2. On the Select Calculator screen, press Assay Range to open the
3. Press Output sample unit, then select the units in
Assay Range Calculator.
which you wish to view the assay range.
26 Qubit
™
Flex Fluorometer User Guide
4. Select the Assay for which you wish to view the assay accuracy range.
5. Enter the sample volume to be used directly in the sample volume text box.
You can also use the + and – buttons or adjust the sample volume wheel.
The Assay Range Calculator displays the Core sample concentration range for
the selected assay and the Extended low and high ranges based on your input.
Qubit™ Flex Fluorometer User Guide 27
Note: Samples with concentrations within the Core range of the assay will
6. Increase or decrease the sample volume to observe how changes in the sample
dsDNA HS Assay range
dsDNA HS Assay range
have <15% relative error for the given sample volume. Samples with
concentrations within the extended range will have <25% relative error for
the given sample volume.
volume affect Core and Extended accuracy ranges for the assay.
for 20 µL sample volume
Note: The sample volume used (1–20 µL) changes the assay accuracy range.
For highest accuracy, use the maximum sample volume that would keep
for 5 µL sample volume
the concentration measurements within the core range.
Note that a different sample volume or assay may be required if the sample
concentration is outside of what the assay can accurately quantify.
28 Qubit
™
Flex Fluorometer User Guide
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