Thermo Scientific invitrogen EVOS FL, invitrogen EVOS FL Color Quick Reference

EVOS

™

FL

EVOS

™

FLColor

Imaging Systems for Fluorescence

and TransmittedLight Applications

Catalog Numbers AMF4300, AMEFC4300

Doc. Part No. ZP-PKGA-0494
Pub. No. MAN0007717
Rev. A.0

For Research Use Only. Not for use in diagnostic procedures.

QUICK REFERENCE

Overview

The EVOS™ FL and EVOS™ FL Color Imaging
Systems have two types of controls: manual

and onscreen. Manual controls include the the
stage X–Y axis knobs, focusing knobs, objective
selection wheel, phase annuli selector, and the
light cube selection lever. Onscreen controls are
located in the control bar at the bottom of the
display screen. The channel bar at the top of the
display screen shows the selected light cube or
transmitted light position.

2

Control bar

Channel bar

Basic Operation

Note: For detailed instructions, refer to the

EVOS™ FL Imaging SystemUser Guide (Pub.No.
MAN0007988), which is provided on the USB
ash drive. You can also dowload it from the
EVOS™ FLImaging System product page at

thermofisher.com.

1. Turn on the instrument using the power

switch  on the right side of the base.

2. Plug a USB ash drive into one of the USB

ports  on the right side of the instrument.

3. Place the sample



on the stage, using a

vessel holder if needed.

4. Set magnication with the objective selection

wheel  on the front of the instrument.

5. Pull the light cube selection lever



all the
way toward the front of the instrument. The
channel bar will highlight the “Transmitted”
position.

CAUTION! UV HAZARD. Avoid exposure
to light beam and use protective shields.
NEVER look directly at UV light!

1

2

3

5

4

6. Turn on illumination with the LIGHT ON

button located on the left side of the

control bar.

7. Focus the sample with focusing knobs



.

8. Optional: To take a picture of the

transmitted light image, click the Capture
button on the control bar.

9. Place the light shield box



on the stage,

over the sample.

10. Move the light cube selection lever



to the
desired uorescence channel. The channel
bar will highlight the selected light cube.

11. With the Find & Focus tab active, turn on

uorescence illumination using the LIGHT

ON button.

12. Adjust the focus as necessary.

13. Adjust the Illumination Intensity slider on

the control bar as needed.

14. Click the Capture button.

15. Repeat steps 10–14 to capture in each

uorescence channel.

16. Click the Overlay tab to show all channels

in color overlay mode.

17. Adjust Brightness and Contrast for

each channel to bring them into desired
balance.

18. Click the Save button to save the color

image (for details, refer to the EVOS™ FL
Imaging SystemUser Guide).

3

6

LIGHT ON button Illumination slider Capture button

7

5

Overlay tab Brightness and Contrast

Save button

Helpful Tips

 In Find & Focus or Actual Mode, the Color

option (for EVOS™ FL, Cat. No. AME4300) can

be turned off to display a grayscale image. This
often shows more details than a color image.

4

Onscreen Controls

1. Active Channel (highlighted)

2. Login Button

3. Control Bar Tabs

• Find & Focus

• Actual

- Exposure Time Slider

• Overlay

- Overlay Color Dialogue Box

4. Light ON/OFF Button

5. Illumination Slider

6. Exposure Time Slider

7. Image Capture Button

8. Color Option

9. Scalebar/Toolbar Options

10. Setting Control Button

11. Save Image Button

12. Info Display Bar

13. Selected Objective

For EVOS™ FL Color (Cat. No. AMEFC4300), use the Color Adjustment button to ne tune your
live image Brightness, Contrast, Saturation, and Hue before capture.

 In Find & Focus Mode, the exposure time is set to 100ms to assist real-time focusing, moving the

stage, etc. The illumination level is approximately 60% of the amount used for image capture to
minimize photobleaching and phototoxicity. Clicking Capture results in brighter illumination and a
longer exposure time during image capture to provide a higher-quality image.

 In Actual Mode, turning on the illumination results in full-powered illumination and actual exposure

times for live viewing of the sample. With longer exposure times (more than 200ms) there will be a
lag between moving the focus knob and seeing the focus change onscreen.

1

4

9

10 11

65

12

13

8

7

3

2

Color option Color Adjustment button

5

Focusing
knobs

Power
switch

Objective
selection
wheel

Condenser
slider slot

Power
input jack

Phase
annulus
selector

Stage
X-axis
knob

USB and
DVI ports

Coarse stage
positioning
knobs

X-axis
stage
brake

Stage
Y-axis
knob

Y-axis
stage
brake

Light cube
selection
lever

Vessel
holder

LCD
screen

Limited Product Warranty

Life Technologies Corporation and/or its afliate(s) warrant their products as set forth in the Life Technologies’ General Terms
and Conditions of Sale found on Life Technologies’ website at www.thermofisher.com/us/en/home/global/terms-and-

conditions.html. If you have any questions, please contact Life Technologies at www.thermofisher.com/support.

Information in this document is subject to change without notice.

DISCLAIMER

TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.

Revision history Pub. No. MAN0007717

Revision Date Description

A.0 02 October 2017

Rebrand document.

1.0 18 March 2013

Basis for this revision.

Important Licensing Information

This product may be covered by one or more Limited Use Label Licenses. By use of this product, you accept the terms and
conditions of all applicable Limited Use Label Licenses.

Manufacturer: Life Technologies Corporation | 22025 20th Ave SE St #100 | Bothell, WA 98021

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02 October 2017