Thermo Scientific HESI-II Probe User Manual

HESI-II Probe

User Guide

70005-97000 Revision C April 2009

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Contents

C

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vii

Precautions for Handling the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . .viii

Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .ix

Chapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1

Chapter 2 Functional Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5

Chapter 3 Removing and Installing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9

Removing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Installing the HESI-II Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Adjusting the Probe Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Chapter 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Flushing the Sample Transfer Line, Sample Tube, and HESI-II Probe . . . . . . . 19

Replacing the Needle Insert. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Chapter 5 Replaceable Parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25

Thermo Scientific HESI-II Probe User Guide v

Preface

This HESI-II Probe User Guide provides you with information on using the

heated-electrospray ionization probe. It also provides procedures for installing and
maintaining the HESI-II probe.

Safety and Special Notices

Make sure you follow the precautionary statements presented in this guide. The safety and
other special notices appear in boxes.

P

Safety and special notices include the following:

CAUTION Highlights hazards to humans, property, or the environment. Each CAUTION
notice is accompanied by an appropriate CAUTION symbol.

IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.

Note Highlights information of general interest.

Tip Highlights helpful information that can make a task easier.

Thermo Scientific HESI-II Probe User Guide vii

Preface

A

S

Hot surface caution

General caution

Hot surface caution

Avoid touching liquid leaking from
the sample inlet while the mass
spectrometer is in operation.

Precautions for Handling the HESI-II Probe

When operating the mass spectrometer in the heated-electrospray mode, do not touch the
heated surfaces of the HESI-II probe or the Ion Max API source housing. Touching the
heated surfaces of the probe or the source housing can cause burns when the probe is
operating at typical temperatures of 350 to 450 °C.

CAUTION AVO ID B URNS . At typical operating temperatures (350 to 450 °C), the
HESI-II probe can severely burn you. Before removing the probe from the API source
housing, allow the probe to cool to room temperature (for approximately 20 minutes)
before you touch it.

When operating the mass spectrometer in the heated-electrospray mode, if you observe liquid
leaking from the sample inlet, place the mass spectrometer in Standby mode before you
tighten the fitting to eliminate the leak.

CAUTION AVO ID E L ECT RIC S HOCK . Do not tighten the probe sample inlet fitting
to eliminate a liquid leak while the mass spectrometer is in operation. If you touch liquid
leaking from the probe sample inlet while the mass spectrometer is in operation, you
might receive an electric shock.

viii HESI-II Probe User Guide Thermo Scientific

Contacting Us

Preface

There are several ways to contact Thermo Fisher Scientific for the information you need.

Y To contact Technical Support

Phone [phone]

Fax [phone]

E-mail [e-mail]

Knowledge base www.thermokb.com

Find software updates and utilities to download at mssupport.thermo.com.

Y To contact Customer Service for ordering information

Phone [phone]

Fax [phone]

E-mail [e-mail]

Web sit e www.thermo.com/ms

Y To copy manuals from the Internet

Go to mssupport.thermo.com and click Customer Manuals in the left margin of the
window.

Y To suggest changes to documentation or to Help

• Complete a brief survey about this document by clicking the link below. Thank you
in advance for your help.

• Send an e-mail message to the Technical Publications Editor at

[e-mail].

Thermo Scientific HESI-II Probe User Guide ix

Introduction

Heated-electrospray ionization (H-ESI) transforms ions in solution into ions in the gas phase
by using electrospray ionization (ESI) in combination with heated auxiliary gas. You can use
H-ESI to analyze any polar compound that makes a preformed ion in solution.

Basic compounds (for example amines) can form a protonated molecule [M + H]
compounds (for example sulphonic acids) can form a deprotonated molecule [M – H]
positive ion polarity mode, the protonated molecule produces a peak at an m/z value of
M + 1, where M equals the mass of the original molecule. In the negative ion polarity mode,
the deprotonated molecule produces a peak at an m/z value of M – 1, where M equals the
mass of the original molecule.

1

+

, and acidic

-

. In the

Because solution chemistry is the primary factor affecting mass spectra in ESI, other common
adducts include sodium ions (Na
yielding an m/z value of [M + 39]
[M + 18]

This chapter describes the principles of the heated-electrospray ionization technique through
use of the HESI-II probe (see Figure 1) and a Thermo Scientific mass spectrometer.

Figure 1. HESI-II probe

+

.

+

), yielding an m/z value of [M + 23]+; potassium ions (K+),

+

; and ammonium ions (NH

+

), yielding an m/z value of

4

Thermo Scientific HESI-II Probe User Guide 1